Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 1 | 1 | 4 | 5 | 1 | 4 | 7 |
Bud | 2 | 1 | 12 | 12 | 12 | 7 | 25 | 26 | 8 | 24 | 0 | 1 | 2 | 2 | 1 | 7 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Site | 3 | 0 | 1 | 2 | 0 | 3 | 7 | 8 | 7 | 9 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 14 | 5 | 3 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 1 | 6 | 5 | 6 | 19 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 1 | 10 | 5 | 11 | 12 | 19 | 23 | 11 | 22 | 5 | 5 | 6 | 7 | 12 | 37 | 30 | 16 | 0 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 11 | 9 | 5 | 3 | 4 | 3 | 3 | 5 | 3 | 0 | 0 | 7 | 2 | 10 | 14 | 6 | 8 | 1 | 0 | 0 | 0 | 2 | 2 |
Endosome | 19 | 1 | 13 | 3 | 9 | 1 | 6 | 4 | 1 | 0 | 0 | 0 | 3 | 4 | 16 | 45 | 37 | 20 | 20 | 14 | 19 | 6 | 7 | 10 |
Golgi | 0 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 | 5 | 21 | 11 | 14 | 9 | 10 | 14 | 7 | 5 | 8 | 9 |
Mitochondria | 212 | 215 | 196 | 167 | 221 | 210 | 386 | 410 | 302 | 378 | 26 | 41 | 93 | 147 | 119 | 24 | 20 | 18 | 197 | 193 | 185 | 82 | 113 | 94 |
Nucleus | 3 | 0 | 1 | 1 | 1 | 4 | 8 | 12 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 2 | 6 | 2 | 1 | 2 | 4 | 12 | 12 | 7 | 12 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 0 | 3 | 1 | 2 | 0 | 0 | 1 |
Nucleolus | 8 | 0 | 0 | 1 | 1 | 2 | 6 | 15 | 2 | 6 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 21 | 6 | 18 | 4 | 7 | 2 | 8 | 6 | 3 | 0 | 0 | 0 | 5 | 13 | 11 | 29 | 19 | 16 | 7 | 9 | 23 | 8 | 12 | 11 |
SpindlePole | 3 | 0 | 17 | 3 | 13 | 8 | 28 | 33 | 5 | 13 | 1 | 2 | 0 | 1 | 2 | 26 | 21 | 9 | 1 | 0 | 1 | 2 | 2 | 2 |
Vac/Vac Membrane | 23 | 28 | 11 | 4 | 16 | 6 | 18 | 10 | 8 | 6 | 0 | 1 | 8 | 15 | 11 | 35 | 36 | 15 | 4 | 9 | 3 | 3 | 4 | 2 |
Unique Cell Count | 242 | 228 | 227 | 180 | 253 | 227 | 422 | 458 | 315 | 402 | 31 | 45 | 116 | 171 | 168 | 218 | 166 | 112 | 255 | 255 | 256 | 112 | 162 | 155 |
Labelled Cell Count | 304 | 289 | 302 | 211 | 297 | 265 | 528 | 565 | 365 | 483 | 33 | 52 | 149 | 204 | 211 | 260 | 205 | 130 | 255 | 255 | 256 | 112 | 162 | 155 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.0 | 4.9 | 4.3 | 4.4 | 4.0 | 3.7 | 4.0 | 3.6 | 3.6 | 4.2 | 3.5 | 5.9 | 5.7 | 5.7 | 5.6 | 6.5 | 7.0 | 4.8 | 5.2 | 6.5 |
Std Deviation (1e-4) | 1.2 | 0.8 | 1.2 | 1.0 | 1.1 | 1.2 | 1.3 | 1.3 | 0.9 | 1.3 | 1.7 | 1.1 | 1.1 | 1.6 | 1.2 | 1.4 | 1.9 | 1.9 | 1.0 | 0.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.15 | -0.29 | -0.41 | -0.28 | -0.43 | -0.46 | -0.21 | -0.5 | 0.27 | 0.22 | 0.22 | 0.2 | 0.4 | 0.51 | -0.02 | 0.1 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | -2.2 | -2.6 | -1.1 | -0.8 | -0.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 3.0 | 1.1 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0.9 | 0 | 0.9 | 1.2 | -0.2 | 1.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.3 | -1.7 | 1.4 | 4.7 | 4.9 | 3.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 5.1 | 0 | 4.9 | 2.6 | 3.7 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.5 | -0.1 | -3.8 | -15.9 | -14.6 | -12.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -1.3 | -0.1 | -0.5 | 1.8 | 1.2 | 1.8 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | -3.0 | -3.3 | -2.9 | 1.6 | 1.7 | 0.2 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 0 | 0.4 | 0.1 | 0.4 | -0.5 | 0.6 | – | – | 0.3 | -0.2 | 1.2 | 4.3 | 4.4 | 3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 0.7 | -1.2 | -0.3 | -1.9 | -1.4 | -2.5 | – | – | 0.8 | 1.6 | 0.7 | 3.9 | 5.1 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7201 | 1.873 | 1.7628 | 1.656 | 1.6578 | 1.7154 | 2.0532 | 2.3848 | 2.2021 | 2.0251 | 1.6629 | 2.405 | 2.9098 | 2.5196 | 2.5593 | 3.5282 | 2.9461 | 2.3059 |
Actin | 0.0334 | 0.0166 | 0.0166 | 0.0425 | 0.0162 | 0.0125 | 0.0978 | 0.022 | 0.0641 | 0.0451 | 0.0131 | 0.0613 | 0.0344 | 0.0358 | 0.0243 | 0.0839 | 0.0183 | 0.0197 |
Bud | 0.0029 | 0.0041 | 0.0033 | 0.0026 | 0.006 | 0.0009 | 0.0021 | 0.0023 | 0.0043 | 0.0049 | 0.0019 | 0.0041 | 0.0006 | 0.0031 | 0.0017 | 0.0083 | 0.0007 | 0.0009 |
Bud Neck | 0.0046 | 0.0016 | 0.0195 | 0.0059 | 0.0018 | 0.0101 | 0.0028 | 0.0026 | 0.0098 | 0.0023 | 0.0018 | 0.0089 | 0.0011 | 0.0026 | 0.0041 | 0.0041 | 0.0008 | 0.0057 |
Bud Periphery | 0.0045 | 0.0092 | 0.0093 | 0.0132 | 0.0335 | 0.0047 | 0.0038 | 0.0042 | 0.0131 | 0.0169 | 0.0037 | 0.0059 | 0.002 | 0.0049 | 0.0059 | 0.0245 | 0.0012 | 0.003 |
Bud Site | 0.0239 | 0.064 | 0.0214 | 0.0281 | 0.0068 | 0.0021 | 0.0171 | 0.0332 | 0.0277 | 0.0137 | 0.0026 | 0.0039 | 0.0024 | 0.0536 | 0.0228 | 0.0067 | 0.0009 | 0.0028 |
Cell Periphery | 0.0038 | 0.0095 | 0.018 | 0.023 | 0.007 | 0.0027 | 0.0013 | 0.0014 | 0.0117 | 0.0025 | 0.0004 | 0.0028 | 0.0022 | 0.0025 | 0.0063 | 0.0013 | 0.0004 | 0.004 |
Cytoplasm | 0.0451 | 0.0467 | 0.0388 | 0.0488 | 0.0102 | 0.0206 | 0.0475 | 0.0237 | 0.027 | 0.0368 | 0.007 | 0.0213 | 0.0161 | 0.012 | 0.0215 | 0.0075 | 0.0238 | 0.033 |
Cytoplasmic Foci | 0.1458 | 0.0286 | 0.0468 | 0.0464 | 0.0476 | 0.0575 | 0.1142 | 0.1105 | 0.0786 | 0.0747 | 0.0675 | 0.0768 | 0.1099 | 0.0783 | 0.0614 | 0.0331 | 0.0734 | 0.0463 |
Eisosomes | 0.0009 | 0.0025 | 0.0043 | 0.0051 | 0.0008 | 0.0006 | 0.0023 | 0.0013 | 0.0076 | 0.0007 | 0.0004 | 0.0012 | 0.0012 | 0.0014 | 0.0037 | 0.0026 | 0.003 | 0.0027 |
Endoplasmic Reticulum | 0.0112 | 0.0092 | 0.0066 | 0.0038 | 0.0035 | 0.0023 | 0.0098 | 0.0012 | 0.0042 | 0.0024 | 0.001 | 0.0021 | 0.0036 | 0.0044 | 0.0021 | 0.0009 | 0.0036 | 0.0034 |
Endosome | 0.1297 | 0.0505 | 0.0777 | 0.0905 | 0.0678 | 0.0673 | 0.1 | 0.0825 | 0.0723 | 0.1021 | 0.0859 | 0.0624 | 0.1367 | 0.0961 | 0.0462 | 0.0921 | 0.165 | 0.0618 |
Golgi | 0.1111 | 0.0436 | 0.042 | 0.0477 | 0.0562 | 0.0517 | 0.0563 | 0.0756 | 0.0503 | 0.058 | 0.0812 | 0.0523 | 0.0792 | 0.0708 | 0.0325 | 0.0154 | 0.0881 | 0.0576 |
Lipid Particles | 0.1217 | 0.0314 | 0.0245 | 0.0107 | 0.0449 | 0.0149 | 0.053 | 0.0249 | 0.0338 | 0.0426 | 0.0241 | 0.0205 | 0.0452 | 0.0226 | 0.0345 | 0.0016 | 0.0326 | 0.0301 |
Mitochondria | 0.1522 | 0.577 | 0.5386 | 0.5255 | 0.5856 | 0.6526 | 0.2142 | 0.4809 | 0.4011 | 0.4371 | 0.4987 | 0.5477 | 0.4063 | 0.4809 | 0.6093 | 0.5723 | 0.4085 | 0.5955 |
None | 0.0765 | 0.0522 | 0.0567 | 0.0729 | 0.005 | 0.0653 | 0.1239 | 0.0467 | 0.0848 | 0.0397 | 0.0608 | 0.0587 | 0.0406 | 0.0721 | 0.0644 | 0.1155 | 0.1268 | 0.0591 |
Nuclear Periphery | 0.0065 | 0.0023 | 0.0013 | 0.0004 | 0.0011 | 0.0006 | 0.0131 | 0.0004 | 0.0035 | 0.0013 | 0.0012 | 0.0007 | 0.002 | 0.004 | 0.0004 | 0.0005 | 0.0008 | 0.0006 |
Nucleolus | 0.0161 | 0.0008 | 0.0023 | 0.0007 | 0.0012 | 0.0006 | 0.0029 | 0.001 | 0.0014 | 0.0004 | 0.0004 | 0.001 | 0.0011 | 0.0015 | 0.0013 | 0.0002 | 0.0005 | 0.0004 |
Nucleus | 0.0085 | 0.0012 | 0.0017 | 0.0008 | 0.0016 | 0.0006 | 0.0084 | 0.0007 | 0.0015 | 0.0011 | 0.0006 | 0.0014 | 0.0008 | 0.0016 | 0.0009 | 0.0003 | 0.0006 | 0.0004 |
Peroxisomes | 0.0683 | 0.0295 | 0.0521 | 0.0164 | 0.0843 | 0.0216 | 0.1025 | 0.0772 | 0.0864 | 0.1042 | 0.1387 | 0.0591 | 0.0908 | 0.0365 | 0.0395 | 0.008 | 0.0418 | 0.0651 |
Punctate Nuclear | 0.0051 | 0.0006 | 0.0008 | 0.0002 | 0.001 | 0.0006 | 0.0155 | 0.0007 | 0.0009 | 0.0012 | 0.0009 | 0.0017 | 0.0053 | 0.0006 | 0.001 | 0.0007 | 0.0013 | 0.0013 |
Vacuole | 0.0234 | 0.014 | 0.0131 | 0.0113 | 0.0122 | 0.0061 | 0.0086 | 0.0055 | 0.0096 | 0.0071 | 0.0041 | 0.0034 | 0.0103 | 0.0109 | 0.0125 | 0.0102 | 0.0046 | 0.0035 |
Vacuole Periphery | 0.0047 | 0.005 | 0.0046 | 0.0036 | 0.0057 | 0.004 | 0.003 | 0.0018 | 0.0063 | 0.0053 | 0.004 | 0.0026 | 0.0084 | 0.0038 | 0.0037 | 0.0103 | 0.0031 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2738 | 6.1228 | 5.452 | 8.584 | 8.8803 | 8.2145 | 7.7384 | 10.8636 | 10.1419 | 12.3078 |
Translational Efficiency | 0.4772 | 0.4627 | 0.7626 | 0.5793 | 0.5408 | 0.8039 | 0.6633 | 0.5804 | 0.7214 | 0.5558 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2383 | 1637 | 230 | 1572 | 76 | 1775 | 226 | 1079 | 2459 | 3412 | 456 | 2651 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 695.81 | 775.15 | 996.24 | 881.02 | 776.33 | 775.22 | 980.29 | 873.74 | 698.30 | 775.19 | 988.33 | 878.06 |
Standard Deviation | 81.74 | 102.14 | 110.33 | 115.33 | 85.29 | 103.65 | 106.84 | 117.58 | 83.03 | 102.92 | 108.91 | 116.31 |
Intensity Change Log 2 | — | 0.155782 | 0.517800 | 0.340481 | — | -0.002064 | 0.336539 | 0.170534 | — | 0.074698 | 0.425055 | 0.253359 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000714 | 0.001942 | 0.005715 | 0.060324 | 0.001354 | 0.001730 | 0.007042 | 0.010808 | 0.000734 | 0.001832 | 0.006373 | 0.040170 |
Bud Neck | 0.014711 | 0.003695 | 0.005064 | 0.017688 | 0.008643 | 0.002659 | 0.013919 | 0.020776 | 0.014523 | 0.003156 | 0.009453 | 0.018945 |
Bud Site | 0.017614 | 0.017132 | 0.020543 | 0.163097* | 0.011924 | 0.008592 | 0.052960 | 0.084535 | 0.017438 | 0.012689 | 0.036609 | 0.131121* |
Cell Periphery | 0.000391 | 0.000289 | 0.000261 | 0.000527 | 0.000722 | 0.000200 | 0.000294 | 0.000262 | 0.000401 | 0.000242 | 0.000278 | 0.000419 |
Cytoplasm | 0.021483 | 0.007435 | 0.004961 | 0.076294 | 0.003163 | 0.006023 | 0.023247 | 0.069256 | 0.020917 | 0.006701 | 0.014024 | 0.073429 |
Cytoplasmic Foci | 0.075418 | 0.047859 | 0.159223 | 0.134270 | 0.053757 | 0.047911 | 0.134380 | 0.145404 | 0.074748 | 0.047886 | 0.146910 | 0.138801 |
Eisosomes | 0.000117 | 0.000091 | 0.000103 | 0.000330 | 0.000126 | 0.000078 | 0.000137 | 0.000138 | 0.000117 | 0.000084 | 0.000120 | 0.000252 |
Endoplasmic Reticulum | 0.001622 | 0.000374 | 0.004227 | 0.005313 | 0.003072 | 0.000205 | 0.004229 | 0.003096 | 0.001667 | 0.000286 | 0.004228 | 0.004411 |
Endosome | 0.077648 | 0.090094 | 0.150666 | 0.039495 | 0.073148 | 0.069399 | 0.109371 | 0.092411 | 0.077509 | 0.079328 | 0.130199 | 0.061033 |
Golgi | 0.100659 | 0.124761* | 0.149395* | 0.076323 | 0.075568 | 0.140929* | 0.103368 | 0.069581 | 0.099883 | 0.133172* | 0.126583* | 0.073579 |
Lipid Particles | 0.009455 | 0.001690 | 0.007651 | 0.002834 | 0.009436 | 0.001891 | 0.008506 | 0.005361 | 0.009455 | 0.001795 | 0.008074 | 0.003863 |
Mitochondria | 0.592884* | 0.618239* | 0.203052* | 0.117878* | 0.598083* | 0.642338* | 0.108181* | 0.153414* | 0.593044* | 0.630776* | 0.156032* | 0.132342* |
Mitotic Spindle | 0.006422 | 0.015646 | 0.100629* | 0.075278 | 0.007241 | 0.013345 | 0.149362* | 0.102404* | 0.006447 | 0.014449 | 0.124782* | 0.086319 |
None | 0.001105 | 0.000270 | 0.000155 | 0.000253 | 0.000420 | 0.000404 | 0.000365 | 0.000670 | 0.001084 | 0.000340 | 0.000259 | 0.000423 |
Nuclear Periphery | 0.000231 | 0.000097 | 0.001038 | 0.000224 | 0.000856 | 0.000094 | 0.001242 | 0.001650 | 0.000251 | 0.000096 | 0.001139 | 0.000805 |
Nuclear Periphery Foci | 0.000344 | 0.000530 | 0.001716 | 0.000590 | 0.001245 | 0.000147 | 0.002378 | 0.001381 | 0.000372 | 0.000331 | 0.002044 | 0.000912 |
Nucleolus | 0.000938 | 0.000264 | 0.001817 | 0.000430 | 0.002992 | 0.000562 | 0.003064 | 0.001924 | 0.001002 | 0.000419 | 0.002435 | 0.001038 |
Nucleus | 0.002685 | 0.000511 | 0.005604 | 0.011722 | 0.002857 | 0.001991 | 0.013629 | 0.012351 | 0.002690 | 0.001281 | 0.009581 | 0.011978 |
Peroxisomes | 0.023332 | 0.034404 | 0.113001* | 0.145859* | 0.117667* | 0.041619 | 0.156738* | 0.085832 | 0.026248 | 0.038157 | 0.134678* | 0.121427* |
Vacuole | 0.027348 | 0.017654 | 0.037551 | 0.064558 | 0.005480 | 0.008815 | 0.086230 | 0.121654 | 0.026672 | 0.013056 | 0.061677 | 0.087797 |
Vacuole Periphery | 0.024878 | 0.017023 | 0.027632 | 0.006712 | 0.022246 | 0.011067 | 0.021360 | 0.017092 | 0.024797 | 0.013925 | 0.024524 | 0.010937 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.82 | -4.85 | -16.49 | -16.08 | -14.33 | -0.53 | -3.57 | -5.91 | -5.92 | -1.72 | -3.85 | -6.25 | -17.33 | -16.75 | -13.58 |
Bud Neck | 8.38 | 6.42 | -2.66 | -12.20 | -9.71 | 2.07 | -1.73 | -3.76 | -11.28 | -2.87 | 9.25 | 3.13 | -3.56 | -16.81 | -7.59 |
Bud Site | 0.23 | -0.81 | -26.94* | -26.56* | -21.62 | 0.50 | -4.33 | -8.81 | -15.88 | -3.42 | 3.26 | -4.67 | -29.00 | -30.83 | -16.55 |
Cell Periphery | 3.12 | 3.03 | -2.59 | -5.49 | -5.04 | 3.29 | 2.49 | 2.84 | -2.01 | 0.28 | 5.52 | 2.63 | -0.11 | -6.21 | -2.79 |
Cytoplasm | 6.99 | 6.77 | -15.63 | -20.41 | -19.79 | -2.04 | -4.62 | -15.86 | -15.33 | -7.16 | 7.75 | 1.37 | -18.39 | -25.56 | -16.37 |
Cytoplasmic Foci | 9.03 | -8.27 | -12.15 | -20.12 | 2.77 | 0.67 | -6.70 | -9.30 | -18.85 | -1.06 | 9.85 | -10.29 | -15.84 | -27.59 | 1.50 |
Eisosomes | 3.85 | 1.25 | -9.90 | -11.22 | -9.97 | 2.57 | -0.76 | -0.68 | -8.14 | 0.26 | 5.62 | -0.72 | -9.85 | -12.87 | -8.60 |
Endoplasmic Reticulum | 3.14 | -1.83 | -6.08 | -9.79 | -0.92 | 1.14 | -0.52 | -0.03 | -8.15 | 1.42 | 3.52 | -2.98 | -5.69 | -12.33 | -0.31 |
Endosome | -2.96 | -7.11 | 12.78 | 14.39 | 11.30 | 0.26 | -1.97 | -0.91 | -3.65 | 2.16 | -0.55 | -7.37 | 5.97 | 7.42 | 10.54 |
Golgi | -4.49 | -4.04 | 6.80 | 10.49 | 6.72 | -4.15 | -1.22 | 0.75 | 13.01 | 3.06 | -7.43 | -2.79 | 8.01 | 16.74 | 6.89 |
Lipid Particles | 7.18 | 0.58 | 6.00 | -3.20 | 1.52 | 2.02 | 0.16 | 0.96 | -4.48 | 1.11 | 7.41 | 0.61 | 5.02 | -5.34 | 2.00 |
Mitochondria | -2.38 | 19.20 | 55.96* | 51.90* | 4.75 | -1.06 | 11.73 | 11.11 | 45.82* | -2.74 | -4.23 | 32.58 | 58.12* | 70.47* | 2.50 |
Mitotic Spindle | -5.80 | -7.95 | -15.90 | -13.22 | 2.10 | -2.72 | -8.95 | -14.52 | -13.81 | 2.76 | -6.63 | -11.94 | -21.69 | -19.17 | 3.70 |
None | 3.75 | 4.30 | 3.60 | -0.64 | -3.81 | 0.00 | -0.41 | -1.29 | -1.29 | -0.84 | 3.29 | 3.28 | 2.66 | -1.17 | -1.21 |
Nuclear Periphery | 2.10 | -2.54 | -1.44 | -4.77 | 2.23 | 1.19 | -0.55 | -0.88 | -2.48 | -0.53 | 2.30 | -3.64 | -2.36 | -3.01 | 0.80 |
Nuclear Periphery Foci | -0.56 | -3.43 | -3.83 | -0.74 | 2.31 | 1.05 | -1.15 | -0.21 | -3.41 | 1.50 | 0.04 | -4.48 | -4.10 | -3.06 | 2.62 |
Nucleolus | 3.35 | -0.87 | 1.78 | -2.30 | 1.24 | 1.09 | -0.21 | 0.45 | -1.92 | 1.22 | 1.89 | -2.18 | -0.51 | -2.18 | 1.94 |
Nucleus | 5.51 | -1.51 | -9.85 | -11.61 | -4.29 | 0.35 | -3.32 | -4.85 | -8.15 | 0.48 | 2.61 | -3.99 | -12.52 | -13.71 | -2.06 |
Peroxisomes | -3.67 | -6.93 | -21.15 | -17.79 | -1.71 | 3.36 | -1.34 | 1.51 | -7.33 | 4.27 | -4.66 | -10.30 | -22.06 | -18.50 | 1.69 |
Vacuole | 5.07 | -2.16 | -14.10 | -16.58 | -7.87 | -2.12 | -8.77 | -21.54 | -21.48 | -3.99 | 8.69 | -6.68 | -21.94 | -27.05 | -6.62 |
Vacuole Periphery | 4.88 | -0.59 | 14.11 | 7.94 | 4.80 | 1.42 | 0.22 | 0.81 | -2.99 | 1.22 | 8.39 | 0.27 | 11.18 | 3.87 | 4.80 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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