Standard name
Human Ortholog
Description Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.14 0 0 0 0 0 0.05 0 0 0.08 0.11
Bud 0 0 0.09 0.05 0 0.05 0 0 – 0 0.06 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0.05 0 0.06 0.11 0.06 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 – 0 0 0 0 0.19 0.08 0.06 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0 0.09 0.13 0 0.06 0.06 0.08 0.07 0.06 0.06 0.05
Golgi 0.07 0.1 0 0 0 0 0 0 – 0 0 0 0.2 0.29 0.32 0.31 0.41 0.43 0.13 0.15 0.06 0.09 0.13 0.14
Mitochondria 0.88 0.88 0.94 0.93 0.9 0.94 0.95 0.93 – 0.93 0.96 0.97 0.63 0.47 0.45 0.08 0.09 0.07 0.66 0.62 0.68 0.63 0.56 0.52
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0 0.05 0.14 0.09 0.06 0.07 0 0.11 0.07 0.09 0.06
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.07 0 0 0.07 0 0.06 0.07 – 0.07 0.06 0 0.22 0.12 0.16 0.16 0.22 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 3 1 1 2 0 1 – 0 0 3 4 22 5 2 0 2 3 6 8 6 18 31
Bud 2 3 27 18 18 34 22 25 – 24 43 37 2 3 4 7 2 1 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 1 1 4 13 12 – 5 9 12 0 0 0 1 0 0 – – – – – –
Cell Periphery 3 2 4 12 4 6 2 5 – 7 6 5 3 6 4 5 0 3 0 0 1 0 0 0
Cytoplasm 4 3 12 18 33 23 22 22 – 24 18 13 5 4 12 10 2 8 1 0 1 0 0 1
Endoplasmic Reticulum 8 14 11 11 9 18 9 14 – 19 6 9 0 29 16 5 1 5 1 1 1 2 3 6
Endosome 3 0 0 4 13 2 1 6 – 0 2 0 10 3 18 11 1 3 12 10 23 10 13 14
Golgi 13 11 2 3 3 0 0 0 – 3 0 1 20 45 64 27 13 23 26 19 20 15 30 40
Mitochondria 170 100 296 334 529 644 825 851 – 778 731 917 62 72 92 7 3 4 131 80 214 106 135 148
Nucleus 3 0 1 1 3 7 9 14 – 3 4 4 0 1 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 6 0 4 2 14 17 27 33 – 44 17 34 0 3 1 0 2 0 1 0 0 2 1 1
Nucleolus 1 0 0 0 8 21 38 56 – 17 16 22 0 0 0 0 1 0 0 0 0 0 1 0
Peroxisomes 3 0 5 6 6 1 2 9 – 4 5 4 8 4 11 12 3 3 14 4 34 12 21 17
SpindlePole 1 0 2 0 5 7 3 10 – 7 11 4 2 0 2 3 1 2 1 0 1 0 0 1
Vac/Vac Membrane 21 8 11 8 41 30 56 63 – 60 47 39 22 18 32 14 7 8 3 3 2 5 8 11
Unique Cell Count 193 114 315 360 586 688 865 915 833 762 944 98 153 203 87 32 54 201 130 315 170 241 283
Labelled Cell Count 242 145 378 419 688 817 1029 1121 995 915 1104 138 210 261 105 36 63 201 130 315 170 241 283


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.2 4.7 4.7 5.0 4.9 4.5 4.8 – 4.8 4.9 4.8 7.6 7.3 7.3 7.5 7.2 7.5 7.7 7.9 8.2
Std Deviation (1e-4) 0.8 1.4 1.1 1.0 1.2 1.2 1.1 1.2 – 1.2 1.2 1.1 1.6 1.0 0.9 1.7 1.3 1.6 1.6 1.6 1.7
Intensity Change (Log2) – – – -0.02 0.07 0.06 -0.09 0.02 – 0.01 0.04 0.02 0.68 0.62 0.62 0.66 0.59 0.67 0.7 0.74 0.79

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 6.1 0 0 0 0
Bud 0 -3.6 -2.2 0 0 – 0 -1.8 0 -2.2 -2.7 -3.1 -0.2 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 1.2 -0.4 -1.2 -1.3 – -0.8 -1.3 -2.7 0 -0.7 1.1 0 0 0
Endoplasmic Reticulum 0 -1.9 -0.8 0 0 – 0 0 0 0 5.6 2.2 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 5.4 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 7.6 9.7 10.3 9.7 0 0
Mitochondria 0 -1.9 -0.2 0 0 – 0 1.4 0 -7.8 -11.6 -12.5 -16.5 -13.2 -15.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.1 1.2 0 0 – 0 1.0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 2.5 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.0 2.2 0.6 2.0 2.2 – 2.3 1.8 0.5 6.0 3.5 4.9 4.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7445 1.0998 1.0615 0.8986 0.5764 0.9894 2.7758 3.2722 3.3231 3.2799 2.3701 3.4259 0.8996 1.1005 0.9475 1.0469 0.8425 0.9481
Actin 0.0294 0.0349 0.0213 0.0065 0.0018 0.0126 0.0256 0.0138 0.0201 0.0502 0.0191 0.0222 0.0232 0.0259 0.0274 0.011 0.0085 0.0162
Bud 0.0007 0.0028 0.0011 0.0033 0.0039 0.0004 0.0006 0.0009 0.0012 0.0018 0.0005 0.0008 0.0006 0.0011 0.0007 0.0008 0.0047 0.0004
Bud Neck 0.0017 0.0012 0.0028 0.0006 0.0008 0.0008 0.0007 0.001 0.0014 0.0006 0.0024 0.0043 0.0018 0.0028 0.0016 0.0212 0.0071 0.0016
Bud Periphery 0.0015 0.0053 0.0038 0.0057 0.0052 0.0007 0.0009 0.0014 0.0027 0.0033 0.0011 0.002 0.0011 0.0013 0.0011 0.0024 0.0074 0.0008
Bud Site 0.005 0.0121 0.0085 0.0075 0.0172 0.0005 0.0018 0.0122 0.0042 0.0011 0.0015 0.0017 0.0037 0.0124 0.0059 0.0018 0.0197 0.0005
Cell Periphery 0.0015 0.0005 0.0017 0.0004 0.0012 0.0002 0.0003 0.0004 0.0015 0.0002 0.0003 0.0007 0.0007 0.0003 0.0007 0.0009 0.0013 0.0002
Cytoplasm 0.0094 0.0042 0.0054 0.0016 0.0018 0.0046 0.0034 0.0096 0.01 0.0031 0.0021 0.0069 0.0053 0.0081 0.0038 0.0017 0.0045 0.0015
Cytoplasmic Foci 0.0587 0.062 0.0426 0.0196 0.0562 0.0273 0.0467 0.0534 0.0374 0.0536 0.0382 0.0431 0.0636 0.0631 0.0329 0.0411 0.0454 0.0251
Eisosomes 0.0043 0.0005 0.0017 0.0002 0.0004 0.0003 0.0005 0.0004 0.0027 0.0003 0.0005 0.0006 0.001 0.0002 0.001 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.0013 0.0006 0.0007 0.0004 0.0002 0.0009 0.0012 0.0012 0.0008 0.0004 0.0003 0.001 0.0017 0.0014 0.0018 0.0001 0.0055 0.0009
Endosome 0.1031 0.1177 0.051 0.0278 0.0654 0.0566 0.1282 0.0943 0.0639 0.085 0.0856 0.0785 0.094 0.1065 0.0677 0.0485 0.126 0.0658
Golgi 0.095 0.147 0.08 0.0351 0.0722 0.075 0.1672 0.1459 0.0989 0.0895 0.1811 0.1116 0.1129 0.1299 0.1006 0.0702 0.1142 0.1048
Lipid Particles 0.0442 0.0122 0.0187 0.004 0.0357 0.0165 0.0286 0.0153 0.0093 0.0107 0.043 0.0081 0.0297 0.0197 0.0096 0.0034 0.0082 0.0165
Mitochondria 0.4955 0.5087 0.6553 0.7703 0.6535 0.7306 0.537 0.5711 0.6628 0.6066 0.5895 0.6268 0.5561 0.544 0.6669 0.7276 0.5852 0.701
None 0.0265 0.0012 0.0219 0.0003 0.0021 0.0067 0.0132 0.0188 0.0171 0.0028 0.0007 0.015 0.0179 0.0124 0.0079 0.0003 0.0019 0.0042
Nuclear Periphery 0.0057 0.0002 0.0013 0.0003 0.0002 0.0003 0.0004 0.0007 0.0002 0.0001 0.0003 0.0017 0.0042 0.001 0.001 0 0.0066 0.0005
Nucleolus 0.0032 0.0004 0.0011 0.0001 0.0015 0.0004 0.0003 0.0002 0.0013 0.0002 0.0004 0.0003 0.0007 0.0006 0.0006 0.0001 0.0006 0.0001
Nucleus 0.0016 0.0005 0.0008 0.0003 0.0005 0.0012 0.0003 0.0005 0.001 0.0002 0.0002 0.0005 0.0007 0.0008 0.0003 0.0001 0.001 0.0002
Peroxisomes 0.0947 0.0769 0.0729 0.1099 0.0708 0.0596 0.0341 0.0532 0.0551 0.0845 0.029 0.0659 0.0722 0.0584 0.0604 0.0661 0.0375 0.054
Punctate Nuclear 0.0022 0.0002 0.0023 0.0001 0.004 0.0005 0.0003 0.0002 0.0006 0.0008 0.0013 0.0019 0.0015 0.0003 0.0001 0.0001 0.0023 0.0002
Vacuole 0.0102 0.0066 0.0028 0.0044 0.003 0.0021 0.0049 0.0034 0.0059 0.0025 0.0013 0.0034 0.0037 0.0057 0.0045 0.0011 0.0065 0.002
Vacuole Periphery 0.0044 0.004 0.002 0.0016 0.0022 0.0023 0.0038 0.0021 0.002 0.0025 0.0015 0.0029 0.0035 0.0042 0.0036 0.0012 0.0059 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1323 11.7749 13.4423 16.2033 13.1369 14.1773 21.7506 19.1615 17.2618 21.3857
Translational Efficiency 1.382 0.974 0.9489 0.9139 0.9551 1.3719 0.8612 0.9105 1.356 0.696

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1757 1629 744 1271 1938 1762 2474 1885 3695 3391 3218 3156

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 737.15 841.10 1055.72 970.19 757.21 756.47 979.91 1028.75 747.67 797.13 997.44 1005.17
Standard Deviation 94.95 117.51 147.28 148.65 95.53 115.43 119.64 150.56 95.78 123.87 130.54 152.52
Intensity Change Log 2 — 0.190319 0.518197 0.396309 — -0.001411 0.371956 0.442127 — 0.096351 0.445948 0.419707

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001851 0.005679 0.017569 0.136583 0.006730 0.006078 0.009636 0.069240 0.004410 0.005886 0.011470 0.096361
Bud Neck 0.001637 0.001913 0.001917 0.002567 0.001444 0.003175 0.000657 0.001960 0.001536 0.002569 0.000948 0.002205
Bud Site 0.005894 0.010800 0.014962 0.086680 0.007137 0.009375 0.015740 0.067726 0.006546 0.010060 0.015560 0.075360
Cell Periphery 0.000570 0.000625 0.000791 0.004832 0.000704 0.000594 0.000387 0.003146 0.000640 0.000609 0.000480 0.003825
Cytoplasm 0.003101 0.003075 0.001871 0.013997 0.002373 0.003736 0.003548 0.015746 0.002719 0.003419 0.003160 0.015041
Cytoplasmic Foci 0.056388 0.063156 0.105775 0.067641 0.043846 0.057266 0.045034 0.066642 0.049810 0.060095 0.059078 0.067045
Eisosomes 0.000135 0.000177 0.000179 0.000661 0.000242 0.000222 0.000138 0.000579 0.000191 0.000200 0.000148 0.000612
Endoplasmic Reticulum 0.001325 0.000810 0.004515 0.006691 0.000721 0.000647 0.000929 0.003324 0.001008 0.000725 0.001758 0.004680
Endosome 0.086005 0.098494 0.099693 0.070974 0.082648 0.091429 0.067108 0.074180 0.084244 0.094823 0.074641 0.072889
Golgi 0.219313 0.227789 0.352799 0.211840 0.195806 0.220963 0.188200 0.246887 0.206984 0.224242 0.226255 0.232773
Lipid Particles 0.006982 0.004087 0.003023 0.000978 0.002879 0.006049 0.000283 0.000504 0.004830 0.005107 0.000916 0.000695
Mitochondria 0.561954 0.526281 0.294068 0.308692 0.612608 0.538327 0.595588 0.375695 0.588522 0.532540 0.525877 0.348711
Mitotic Spindle 0.002424 0.008381 0.019254 0.021932 0.002503 0.004248 0.016422 0.011017 0.002465 0.006234 0.017077 0.015413
None 0.000333 0.000229 0.000334 0.000244 0.000173 0.000214 0.000200 0.000193 0.000249 0.000221 0.000231 0.000213
Nuclear Periphery 0.000140 0.000082 0.000786 0.000189 0.000037 0.000064 0.000126 0.000043 0.000086 0.000073 0.000279 0.000102
Nuclear Periphery Foci 0.000233 0.000233 0.001756 0.000756 0.000139 0.000218 0.000160 0.000131 0.000184 0.000225 0.000529 0.000383
Nucleolus 0.000780 0.000261 0.000101 0.000163 0.000168 0.001113 0.000023 0.000020 0.000459 0.000704 0.000041 0.000078
Nucleus 0.000587 0.000351 0.000141 0.000192 0.000163 0.000677 0.000061 0.000187 0.000365 0.000520 0.000079 0.000189
Peroxisomes 0.018996 0.021083 0.010084 0.027772 0.018500 0.037117 0.012629 0.032145 0.018736 0.029414 0.012041 0.030384
Vacuole 0.009818 0.010505 0.030863 0.019851 0.006563 0.007782 0.019047 0.016129 0.008111 0.009090 0.021779 0.017628
Vacuole Periphery 0.021533 0.015985 0.039517 0.016765 0.014616 0.010708 0.024084 0.014504 0.017905 0.013243 0.027653 0.015414

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.55 -6.57 -19.41 -18.76 -16.24 0.57 -2.17 -15.91 -16.14 -14.98 -2.04 -6.52 -24.93 -24.45 -22.41
Bud Neck -0.72 -0.74 -2.56 -2.30 -2.00 -3.42 3.08 -1.86 2.62 -9.09 -3.22 2.73 -2.98 1.30 -9.60
Bud Site -3.52 -4.50 -19.09 -17.70 -15.81 -1.83 -7.07 -18.95 -18.00 -16.02 -3.81 -8.88 -26.84 -25.11 -22.67
Cell Periphery -0.37 -1.45 -7.69 -7.55 -7.24 0.68 2.13 -5.72 -6.27 -6.87 0.28 1.67 -9.44 -9.69 -10.23
Cytoplasm 0.03 2.30 -9.80 -10.20 -11.64 -2.68 -2.74 -13.49 -11.42 -12.00 -1.82 -1.17 -16.47 -15.35 -15.80
Cytoplasmic Foci -2.49 -10.47 -3.57 -1.37 7.50 -5.83 -0.55 -9.19 -3.52 -8.78 -5.79 -4.73 -8.79 -3.36 -3.65
Eisosomes -2.73 -3.20 -16.22 -14.04 -14.34 0.87 4.20 -9.74 -11.61 -13.76 -0.67 2.72 -17.62 -17.85 -19.19
Endoplasmic Reticulum 1.53 -4.64 -6.47 -7.61 -2.21 0.41 -1.87 -4.64 -4.64 -4.28 1.52 -3.41 -7.65 -8.53 -6.09
Endosome -2.83 -2.90 3.77 6.38 6.19 -2.20 4.79 2.52 4.63 -2.41 -3.56 3.67 4.42 7.78 0.73
Golgi -1.03 -11.31 0.86 1.81 11.44 -3.33 1.13 -6.62 -3.22 -8.03 -3.10 -3.30 -4.36 -1.39 -1.02
Lipid Particles 2.53 3.70 6.35 4.66 3.64 -2.67 4.92 4.46 5.20 -2.90 -0.35 7.29 7.82 6.90 1.41
Mitochondria 3.01 18.53 20.59 17.33 -0.95 6.63 1.66 22.00 14.55 21.36 6.86 7.36 29.53 22.02 20.38
Mitotic Spindle -5.95 -6.60 -8.44 -5.49 -0.85 -2.43 -11.17 -7.59 -5.60 3.40 -6.18 -13.16 -11.27 -7.37 1.00
None 1.66 0.01 1.71 -0.29 3.44 -1.23 -1.41 -0.94 0.71 0.58 0.80 0.74 1.34 0.37 1.55
Nuclear Periphery 1.93 -2.40 -0.76 -1.80 2.18 -1.53 -3.06 -0.92 1.18 2.84 0.79 -2.87 -0.58 -1.12 2.52
Nuclear Periphery Foci -0.02 -2.32 -1.49 -1.49 1.35 -1.04 -0.51 0.18 1.24 1.05 -0.88 -2.23 -1.40 -1.07 0.71
Nucleolus 1.24 1.61 1.47 0.69 -0.32 -1.76 1.38 1.41 2.07 0.40 -0.73 2.06 1.86 2.23 -0.67
Nucleus 0.96 2.04 1.83 1.22 -0.67 -1.58 1.75 -0.40 1.46 -1.91 -0.75 2.67 1.49 1.79 -2.35
Peroxisomes -0.89 4.11 -3.03 -2.29 -6.41 -6.22 3.00 -4.89 1.45 -7.64 -5.54 4.53 -5.75 -0.43 -9.60
Vacuole -0.63 -8.72 -5.42 -4.96 3.80 -1.33 -11.57 -7.37 -5.88 1.87 -1.38 -13.54 -8.79 -7.52 3.03
Vacuole Periphery 3.68 -5.98 2.66 -0.52 7.57 3.54 -6.76 0.10 -3.48 6.91 5.04 -7.47 2.39 -2.43 9.55
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
Localization
Cell Percentages mitochondrion (72%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Msp1

Msp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available