Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.06 0.05 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0 0
Cytoplasm 0.51 0.69 0.89 0.84 0.73 0.58 0.41 0.48 0.38 0.34 0.32 0.39 0.95 0.97 0.98 – 0.52 0.49 0.14 0.12 0.14 0.21 0.23 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.07 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.1 0.06 0.07 0.05 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Mitochondria 0.54 0.22 0 0.15 0.1 0.38 0.6 0.49 0.69 0.68 0.82 0.62 0 0 0 – 0.08 0.12 0.61 0.61 0.65 0.59 0.49 0.51
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.09 0.09 0.08 0.09 0.05 0.09 0.05 0.07 0 0 0 – 0.17 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.09 0.12 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 1 0 0 0 0 1 0 1 0 0 3 2 1 – 0 0 6 4 3 4 11 14
Bud 7 1 3 2 2 2 16 16 15 10 14 13 1 0 0 – 0 3 2 0 0 1 3 10
Bud Neck 4 0 4 5 15 4 10 10 5 9 2 5 12 7 6 – 4 1 1 2 0 1 5 11
Bud Site 1 0 0 0 0 1 3 5 2 4 3 0 0 0 0 – 1 2 – – – – – –
Cell Periphery 1 1 4 1 1 0 4 1 1 1 0 0 2 2 10 – 6 0 1 0 0 0 2 6
Cytoplasm 117 90 99 154 194 132 132 124 115 82 83 122 412 474 587 – 45 33 38 22 21 30 78 91
Endoplasmic Reticulum 4 5 0 1 2 0 1 1 0 1 0 0 3 1 2 – 8 5 7 1 4 2 4 5
Endosome 0 6 2 0 6 1 2 4 0 1 0 0 3 1 5 – 5 7 14 13 7 5 18 12
Golgi 0 1 0 0 2 0 0 0 0 0 0 0 1 0 1 – 1 5 1 3 0 0 3 2
Mitochondria 123 29 4 28 27 85 193 128 209 165 214 192 2 1 0 – 7 8 163 110 102 85 165 217
Nucleus 1 0 0 1 3 0 0 6 10 3 2 1 0 0 0 – 4 0 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 1
Peroxisomes 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 – 2 0 1 0 1 1 1 3
SpindlePole 8 3 1 12 25 20 25 24 16 22 13 23 5 5 7 – 15 9 8 6 5 5 13 15
Vac/Vac Membrane 4 4 3 1 11 6 11 2 2 2 4 10 3 7 4 – 8 8 10 11 2 3 13 20
Unique Cell Count 228 131 111 184 267 226 321 260 301 241 261 310 434 487 602 87 67 270 180 158 145 335 431
Labelled Cell Count 270 145 121 205 288 252 397 322 375 302 335 367 447 500 623 106 81 270 180 158 145 335 431


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.3 4.9 4.3 4.6 3.7 3.3 3.8 3.1 3.3 3.1 3.5 4.8 5.1 4.9 – 6.3 6.0 4.6 4.9 5.4
Std Deviation (1e-4) 0.5 0.8 1.3 1.5 1.6 1.3 1.2 1.1 1.0 1.1 1.6 1.2 1.0 1.2 1.0 – 3.1 2.4 1.4 1.3 2.1
Intensity Change (Log2) – – – -0.17 -0.08 -0.41 -0.54 -0.35 -0.66 -0.55 -0.63 -0.45 -0.02 0.07 -0.0 – 0.38 0.3 -0.08 0.02 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6566 -0.9886 -0.8932 -0.924 -0.618 -0.9568 -0.4312 -0.3584 -0.2834 -0.7875 -0.04 -0.468 -1.2113 -1.2665 -0.9949 -0.4786 -0.9796 -1.089
Actin 0.0506 0.0036 0.0161 0.0519 0.0735 0.0121 0.034 0.0013 0.0052 0.0033 0.0079 0.009 0.0123 0.0073 0.0408 0.0212 0.0073 0.0115
Bud 0.0019 0.0012 0.0017 0.003 0.0008 0.0022 0.0006 0.0011 0.0017 0.0005 0.0008 0.0026 0.0023 0.0018 0.0017 0.0239 0.0026 0.002
Bud Neck 0.003 0.0005 0.0016 0.0024 0.0013 0.0057 0.0091 0.0006 0.0047 0.0008 0.0006 0.0041 0.004 0.0011 0.0063 0.0046 0.0013 0.0126
Bud Periphery 0.0024 0.0019 0.0057 0.0038 0.0016 0.0065 0.0015 0.0031 0.0036 0.0035 0.0025 0.006 0.0047 0.0029 0.0042 0.016 0.0071 0.0053
Bud Site 0.0157 0.0145 0.0142 0.0093 0.0012 0.0016 0.0042 0.0079 0.0109 0.0008 0.0012 0.0008 0.0139 0.0185 0.0087 0.0205 0.0031 0.0007
Cell Periphery 0.0011 0.0009 0.001 0.0005 0.0002 0.0005 0.001 0.0012 0.0008 0.0012 0.0004 0.0004 0.0013 0.0014 0.001 0.001 0.0025 0.0005
Cytoplasm 0.0688 0.111 0.0667 0.0829 0.0671 0.1196 0.0525 0.1083 0.109 0.1205 0.0401 0.1566 0.0949 0.207 0.1771 0.173 0.2264 0.2351
Cytoplasmic Foci 0.0955 0.0683 0.0816 0.0638 0.1434 0.0865 0.0542 0.0791 0.0518 0.1198 0.076 0.0942 0.117 0.1343 0.0932 0.1307 0.073 0.1098
Eisosomes 0.0015 0.0009 0.0007 0.0004 0.0004 0.0004 0.0007 0.0006 0.0005 0.0015 0.0006 0.0002 0.0013 0.0011 0.0021 0.0006 0.0016 0.0004
Endoplasmic Reticulum 0.0065 0.0038 0.0023 0.0018 0.0029 0.0019 0.0078 0.003 0.0022 0.0087 0.0012 0.003 0.0032 0.0073 0.0065 0.0039 0.009 0.0034
Endosome 0.0467 0.0315 0.0296 0.0174 0.0659 0.0217 0.0493 0.0381 0.0394 0.0727 0.0508 0.0346 0.0561 0.0789 0.0687 0.0861 0.1201 0.0697
Golgi 0.0111 0.0053 0.0052 0.006 0.0178 0.007 0.0073 0.0049 0.0035 0.0031 0.0069 0.0058 0.0136 0.0117 0.0132 0.016 0.0169 0.0107
Lipid Particles 0.0239 0.0135 0.0116 0.0034 0.0314 0.0083 0.0191 0.0105 0.0062 0.0054 0.0091 0.0058 0.0395 0.016 0.017 0.014 0.0096 0.0068
Mitochondria 0.0328 0.0188 0.0333 0.0496 0.0181 0.0356 0.0247 0.0261 0.0128 0.0815 0.0341 0.0319 0.0399 0.0168 0.0467 0.0611 0.0487 0.0472
None 0.5646 0.6954 0.6839 0.6701 0.4528 0.6581 0.6835 0.6894 0.7279 0.5423 0.7384 0.6134 0.5582 0.4582 0.4777 0.3763 0.4444 0.4531
Nuclear Periphery 0.0134 0.0014 0.0018 0.0013 0.0257 0.0025 0.0139 0.001 0.0024 0.0096 0.0021 0.0044 0.0007 0.0023 0.002 0.0013 0.0043 0.0012
Nucleolus 0.0033 0.001 0.0014 0.0014 0.0009 0.0006 0.0014 0.001 0.0008 0.0005 0.0012 0.0006 0.0031 0.0009 0.0016 0.0036 0.0015 0.0005
Nucleus 0.0046 0.0014 0.0017 0.004 0.0021 0.0014 0.0036 0.0021 0.0021 0.0035 0.0011 0.0031 0.0014 0.0016 0.0016 0.0084 0.003 0.0014
Peroxisomes 0.0311 0.0152 0.0288 0.0135 0.0491 0.0136 0.0121 0.0093 0.0061 0.0038 0.0158 0.0145 0.0195 0.012 0.0186 0.0248 0.0043 0.0173
Punctate Nuclear 0.0143 0.0044 0.0052 0.0098 0.0359 0.0105 0.0125 0.0022 0.0027 0.0023 0.0009 0.0042 0.0025 0.0043 0.0028 0.002 0.002 0.0022
Vacuole 0.0052 0.0046 0.0045 0.0027 0.0054 0.0028 0.005 0.0078 0.0047 0.0123 0.0047 0.0038 0.0093 0.0118 0.0063 0.009 0.008 0.0056
Vacuole Periphery 0.002 0.0011 0.0013 0.001 0.0025 0.0009 0.0023 0.0012 0.0012 0.0026 0.0036 0.0011 0.0013 0.003 0.0022 0.0022 0.0034 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1791 8.3503 13.1671 13.5322 12.8679 22.1726 14.7752 12.8215 14.129 17.009
Translational Efficiency 0.3074 0.6652 0.3118 0.429 0.4681 0.2411 0.3668 0.4872 0.4213 0.3696

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
198 450 1431 1568 1795 1648 140 1508 1993 2098 1571 3076

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.84 675.18 833.61 793.59 651.47 702.28 889.04 832.44 656.08 696.47 838.55 812.64
Standard Deviation 70.81 99.15 98.94 113.61 72.34 84.06 93.11 105.46 73.51 88.22 99.69 111.40
Intensity Change Log 2 — -0.047624 0.256476 0.185498 — 0.108348 0.448550 0.353648 — 0.029789 0.352408 0.269132

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000664 0.001684 0.000357 0.006287 0.000569 0.001293 0.000422 0.003156 0.000578 0.001377 0.000363 0.004752
Bud Neck 0.059345 0.058118 0.009311 0.037853 0.041175 0.049076 0.007242 0.039501 0.042980 0.051016 0.009127 0.038661
Bud Site 0.015203 0.037461 0.006675 0.082788 0.013479 0.026616 0.007133 0.065542 0.013650 0.028942 0.006716 0.074333
Cell Periphery 0.000217 0.000388 0.000135 0.000252 0.000261 0.000318 0.000308 0.000183 0.000257 0.000333 0.000150 0.000218
Cytoplasm 0.170494* 0.085848 0.332893* 0.123498* 0.170856* 0.096430 0.242037* 0.117540* 0.170820* 0.094160 0.324797* 0.120577*
Cytoplasmic Foci 0.382913* 0.272479* 0.047130 0.164595 0.374683* 0.287239* 0.156430 0.181921* 0.375500* 0.284073* 0.056871 0.173089
Eisosomes 0.000472 0.000234 0.000057 0.000171 0.000408 0.000290 0.000237 0.000144 0.000415 0.000278 0.000073 0.000158
Endoplasmic Reticulum 0.000956 0.001141 0.004380 0.004157 0.002680 0.000697 0.003453 0.003358 0.002509 0.000792 0.004297 0.003766
Endosome 0.070417 0.095033 0.008698 0.058489 0.048292 0.084068 0.027814 0.083664 0.050490 0.086420 0.010402 0.070831
Golgi 0.024109 0.036772 0.000427 0.027468 0.036009 0.059763 0.000699 0.027894 0.034827 0.054832 0.000452 0.027676
Lipid Particles 0.019168 0.024645 0.005900 0.009985 0.033691 0.013720 0.016329 0.009335 0.032248 0.016063 0.006829 0.009666
Mitochondria 0.069278 0.131601* 0.002120 0.077453 0.129524* 0.197971* 0.002868 0.078671 0.123539* 0.183736* 0.002187 0.078050
Mitotic Spindle 0.005755 0.018579 0.012135 0.087263 0.002801 0.010405 0.002684 0.079555 0.003094 0.012158 0.011293 0.083484
None 0.000561 0.002697 0.005089 0.001989 0.001298 0.001043 0.004167 0.001185 0.001225 0.001398 0.005007 0.001595
Nuclear Periphery 0.000335 0.000865 0.004148 0.001791 0.000585 0.000280 0.002120 0.001762 0.000560 0.000405 0.003967 0.001777
Nuclear Periphery Foci 0.000297 0.001011 0.001644 0.001587 0.000247 0.000327 0.003072 0.001204 0.000252 0.000474 0.001771 0.001399
Nucleolus 0.001126 0.006575 0.003660 0.003567 0.001283 0.002495 0.007183 0.003762 0.001268 0.003370 0.003974 0.003663
Nucleus 0.043295 0.039338 0.487387* 0.050909 0.046622 0.022959 0.381188* 0.042304 0.046292 0.026472 0.477923* 0.046690
Peroxisomes 0.072177 0.120890* 0.002653 0.093463 0.037333 0.085489 0.010218 0.072221 0.040795 0.093083 0.003328 0.083049
Vacuole 0.057720 0.055065 0.064194 0.155140 0.049782 0.051106 0.123366 0.174256 0.050571 0.051955 0.069467 0.164511
Vacuole Periphery 0.005497 0.009574 0.001007 0.011295 0.008422 0.008413 0.001029 0.012842 0.008131 0.008662 0.001009 0.012054

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.82 1.49 -6.94 -5.33 -7.37 -3.42 0.75 -7.73 -4.73 -7.89 -4.34 2.06 -9.44 -7.10 -9.85
Bud Neck 0.15 5.94 2.45 3.50 -13.57 -2.32 12.56 0.88 3.15 -12.23 -2.51 15.47 1.88 4.58 -18.81
Bud Site -4.68 2.73 -13.53 -7.68 -18.71 -6.27 2.36 -14.46 -9.85 -13.72 -7.92 4.63 -21.32 -14.20 -24.06
Cell Periphery -1.33 2.77 -0.40 1.24 -4.69 -1.15 -1.56 4.56 3.13 5.25 -1.58 6.35 3.04 2.87 -3.77
Cytoplasm 4.79 -8.31 2.50 -4.86 21.75 12.00 -3.41 6.63 -4.83 5.67 13.30 -16.93 7.59 -6.92 24.01
Cytoplasmic Foci 6.01 21.26 13.94 11.12 -22.52 11.59 16.28 28.68 16.20 -1.63 13.28 56.13 35.51 21.28 -26.97
Eisosomes 3.03 5.30 3.93 3.83 -15.70 3.31 5.49 10.19 5.61 5.35 4.37 13.89 10.65 5.90 -13.49
Endoplasmic Reticulum -0.56 -11.57 -7.14 -7.41 2.29 5.87 -2.05 -1.34 -7.20 0.87 5.63 -7.07 -3.34 -11.02 3.92
Endosome -2.13 6.88 1.90 5.90 -17.88 -9.42 3.48 -7.32 1.81 -8.03 -10.07 17.54 -4.76 6.36 -25.19
Golgi -2.00 4.64 0.09 3.28 -13.52 -7.65 19.50 4.73 11.62 -12.95 -7.27 20.13 5.32 12.70 -18.80
Lipid Particles -1.17 4.49 3.14 3.82 -3.86 10.12 6.06 12.83 3.86 2.98 8.31 14.82 13.43 5.44 -3.26
Mitochondria -4.06 6.09 0.12 5.76 -16.57 -8.40 24.49 9.55 17.15 -15.27 -8.25 26.26 10.33 18.75 -23.25*
Mitotic Spindle -2.38 -1.42 -12.58 -11.58 -14.34 -5.32 0.16 -16.56 -14.53 -15.92 -6.33 -4.21 -23.67 -20.11 -18.81
None -2.90 -7.61 -1.84 1.07 3.47 1.05 -2.28 0.49 -0.40 2.39 -0.89 -6.57 -0.66 -0.06 5.22
Nuclear Periphery -2.51 -14.28 -5.60 -3.58 5.81 2.64 -5.34 -4.26 -5.46 0.79 1.40 -13.89 -6.31 -7.40 6.91
Nuclear Periphery Foci -2.28 -8.32 -5.42 -1.70 0.95 -1.26 -5.65 -8.26 -7.18 3.71 -2.66 -10.46 -8.99 -6.55 2.63
Nucleolus -3.29 -4.94 -4.75 1.85 -0.20 -3.17 -2.30 -5.34 -2.53 1.16 -4.50 -5.57 -7.07 -0.65 -0.06
Nucleus 0.39 -44.76 -1.91 -2.71 51.15 7.86 -12.82 -0.38 -7.27 12.73 6.80 -54.70 -2.04 -9.18 54.10
Peroxisomes -3.93 7.29 -1.49 3.70 -20.58* -12.20 9.11 -7.87 3.50 -13.98 -13.78 18.82 -11.17 3.62 -27.42*
Vacuole 0.45 -2.82 -14.71 -17.99 -16.47 -0.54 -6.42 -23.80 -23.09 -2.94 -0.55 -8.88 -31.14 -30.40 -20.28
Vacuole Periphery -2.19 3.96 -2.89 -0.22 -9.23 0.05 12.45 -2.61 -2.75 -11.77 -0.63 13.06 -2.72 -2.23 -14.60
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
Localization
Cell Percentages mitochondrion (52%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mtq1

Mtq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available