Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 2 | 18 | 30 | 17 | 11 | 12 | 13 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | 3 | 2 | 5 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 2 | 2 | 2 | 5 | 18 | 38 | 24 | 22 | 18 | 15 | 5 | 3 | 3 | 19 | 11 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 121 | 166 | 254 | 180 | 237 | 326 | 449 | 487 | 274 | 245 | 198 | 174 | 326 | 367 | 448 | 365 | 268 | 189 | 114 | 170 | 261 | 169 | 276 | 331 |
Endoplasmic Reticulum | 0 | 3 | 12 | 4 | 9 | 25 | 41 | 89 | 34 | 35 | 15 | 55 | 25 | 14 | 13 | 13 | 11 | 6 | 2 | 1 | 5 | 26 | 49 | 53 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 18 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Mitochondria | 1 | 0 | 0 | 0 | 1 | 9 | 31 | 23 | 70 | 46 | 85 | 38 | 1 | 0 | 0 | 1 | 3 | 0 | 3 | 0 | 2 | 2 | 1 | 4 |
Nucleus | 1 | 1 | 1 | 1 | 3 | 0 | 0 | 6 | 2 | 3 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 0 | 5 | 3 | 15 | 42 | 8 | 20 | 8 | 6 | 1 | 2 | 2 | 15 | 9 | 16 | 2 | 1 | 6 | 10 | 16 | 15 |
Unique Cell Count | 122 | 170 | 263 | 183 | 249 | 357 | 539 | 658 | 375 | 337 | 281 | 263 | 350 | 380 | 463 | 382 | 284 | 209 | 127 | 176 | 280 | 214 | 355 | 417 |
Labelled Cell Count | 123 | 175 | 272 | 188 | 259 | 371 | 585 | 740 | 432 | 386 | 343 | 307 | 359 | 389 | 467 | 415 | 303 | 224 | 127 | 176 | 280 | 214 | 355 | 417 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.2 | 20.5 | 16.7 | 15.9 | 14.9 | 12.4 | 12.2 | 12.3 | 10.2 | 9.9 | 8.6 | 9.5 | 22.3 | 22.7 | 21.9 | 25.2 | 24.6 | 23.7 | 19.4 | 19.9 | 21.7 |
Std Deviation (1e-4) | 4.8 | 3.4 | 2.6 | 2.8 | 2.5 | 1.9 | 1.9 | 2.0 | 2.1 | 1.8 | 1.6 | 1.8 | 3.2 | 3.7 | 3.6 | 8.0 | 8.0 | 8.2 | 2.7 | 3.7 | 4.0 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.17 | -0.43 | -0.46 | -0.45 | -0.71 | -0.76 | -0.97 | -0.81 | 0.41 | 0.44 | 0.38 | 0.59 | 0.56 | 0.5 | 0.21 | 0.25 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.0 | 3.5 | 3.5 | 0 | 3.4 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 2.2 | 3.4 | 3.5 | 3.6 | 3.5 | 3.2 | 0 | 0 | 0 | 3.0 | 2.4 | 3.0 |
Cytoplasm | 1.1 | -0.8 | -2.6 | -5.4 | -7.8 | -7.7 | -7.8 | -8.1 | -9.0 | -1.9 | 0 | 0.1 | -0.7 | -1.2 | -2.8 |
Endoplasmic Reticulum | -1.3 | -0.5 | 1.3 | 1.6 | 3.9 | 2.2 | 2.6 | 0.4 | 5.6 | 1.3 | -0.6 | -1.2 | -0.7 | -0.4 | -1.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.0 | 3.1 | 7.4 | 6.2 | 9.7 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.5 | 3.3 | 0 | 3.0 | 1.4 | 0 | 0 | 0 | 0 | 2.1 | 1.6 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.0184 | 21.0674 | 18.7755 | 17.758 | 13.6344 | 18.8167 | 16.3364 | 22.3955 | 20.8474 | 19.5396 | 18.6088 | 20.4347 | 17.0733 | 23.0115 | 21.4769 | 18.7621 | 19.9496 | 20.7261 |
Actin | 0.0255 | 0.0016 | 0.0112 | 0.001 | 0.0493 | 0.0012 | 0.0095 | 0.0015 | 0.007 | 0.0004 | 0.0001 | 0.001 | 0.0123 | 0.0003 | 0.0081 | 0.0269 | 0.0002 | 0.0013 |
Bud | 0.0018 | 0.001 | 0.0018 | 0.0025 | 0.001 | 0.0013 | 0.0007 | 0.0005 | 0.0009 | 0.0008 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0009 | 0.0007 | 0.0001 | 0.0013 |
Bud Neck | 0.0016 | 0.0006 | 0.0009 | 0.0013 | 0.0012 | 0.0017 | 0.0006 | 0.0002 | 0.0006 | 0.0006 | 0.0003 | 0.0008 | 0.0007 | 0.0002 | 0.0005 | 0.0009 | 0.0004 | 0.0009 |
Bud Periphery | 0.0034 | 0.0007 | 0.0014 | 0.0015 | 0.0012 | 0.0019 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.002 |
Bud Site | 0.0046 | 0.0043 | 0.0014 | 0.0008 | 0.0014 | 0.0004 | 0.0009 | 0.0077 | 0.0028 | 0.0008 | 0.0001 | 0.0001 | 0.0009 | 0.001 | 0.0004 | 0.0019 | 0 | 0.0001 |
Cell Periphery | 0.0017 | 0.0017 | 0.0013 | 0.0019 | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 |
Cytoplasm | 0.6041 | 0.8953 | 0.8389 | 0.7126 | 0.5669 | 0.8102 | 0.8066 | 0.9643 | 0.9149 | 0.8417 | 0.915 | 0.8848 | 0.8086 | 0.9634 | 0.8865 | 0.7407 | 0.8698 | 0.8368 |
Cytoplasmic Foci | 0.0194 | 0.0046 | 0.0078 | 0.0091 | 0.0475 | 0.0122 | 0.0144 | 0.0049 | 0.0083 | 0.0169 | 0.0116 | 0.0116 | 0.0093 | 0.0058 | 0.0094 | 0.0134 | 0.0115 | 0.011 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0887 | 0.0349 | 0.0282 | 0.0414 | 0.0298 | 0.0486 | 0.0223 | 0.0034 | 0.0037 | 0.0089 | 0.0046 | 0.0116 | 0.069 | 0.0062 | 0.0204 | 0.0117 | 0.0258 | 0.067 |
Endosome | 0.0622 | 0.0158 | 0.035 | 0.0912 | 0.0938 | 0.0487 | 0.0774 | 0.0093 | 0.0275 | 0.0699 | 0.0453 | 0.058 | 0.038 | 0.0105 | 0.0342 | 0.0602 | 0.0722 | 0.0461 |
Golgi | 0.0139 | 0.0022 | 0.0047 | 0.0062 | 0.0199 | 0.0098 | 0.0183 | 0.0008 | 0.0029 | 0.0034 | 0.0008 | 0.009 | 0.0063 | 0.0009 | 0.0032 | 0.0062 | 0.0017 | 0.0059 |
Lipid Particles | 0.0175 | 0.0003 | 0.0007 | 0.0006 | 0.0311 | 0.0004 | 0.0017 | 0 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0024 | 0.0001 | 0.0013 | 0.0045 | 0 | 0.0001 |
Mitochondria | 0.0377 | 0.0124 | 0.014 | 0.0025 | 0.0673 | 0.024 | 0.0045 | 0.0003 | 0.0085 | 0.0009 | 0.0004 | 0.0079 | 0.002 | 0.0002 | 0.0017 | 0.0031 | 0.0007 | 0.0096 |
None | 0.0021 | 0.0024 | 0.0007 | 0.0004 | 0.0026 | 0.0009 | 0.0017 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.002 | 0.0003 | 0.0004 | 0.0019 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0182 | 0.003 | 0.0036 | 0.0046 | 0.0075 | 0.0061 | 0.0074 | 0.0008 | 0.0009 | 0.0033 | 0.0017 | 0.0013 | 0.0095 | 0.0007 | 0.0027 | 0.0093 | 0.0017 | 0.0033 |
Nucleolus | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0025 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0005 | 0 | 0 |
Nucleus | 0.0042 | 0.0013 | 0.0017 | 0.0021 | 0.0021 | 0.0049 | 0.0023 | 0.0008 | 0.0009 | 0.0015 | 0.0008 | 0.0007 | 0.0038 | 0.0007 | 0.0013 | 0.0039 | 0.0006 | 0.0011 |
Peroxisomes | 0.0138 | 0.0001 | 0.0008 | 0.0001 | 0.0345 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.001 | 0.0008 | 0 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0035 | 0.0008 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0054 | 0 | 0.0002 | 0.0062 | 0.0001 | 0.0001 |
Vacuole | 0.0584 | 0.015 | 0.0371 | 0.0957 | 0.0283 | 0.0159 | 0.025 | 0.0041 | 0.016 | 0.0383 | 0.0138 | 0.0066 | 0.0215 | 0.0079 | 0.0216 | 0.0839 | 0.0097 | 0.0082 |
Vacuole Periphery | 0.019 | 0.0027 | 0.0083 | 0.0243 | 0.0096 | 0.0078 | 0.0048 | 0.0009 | 0.0038 | 0.0115 | 0.0048 | 0.0054 | 0.0054 | 0.0012 | 0.0052 | 0.0224 | 0.0049 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 97.8653 | 84.2169 | 80.1845 | 86.0489 | 103.7403 | 81.2585 | 81.7495 | 73.6109 | 85.0677 | 98.8686 |
Translational Efficiency | 1.0868 | 1.064 | 0.9492 | 1.0125 | 0.9179 | 1.2725 | 0.9369 | 0.9666 | 0.8937 | 0.9438 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1924 | 1143 | 419 | 1035 | 1346 | 993 | 1674 | 287 | 3270 | 2136 | 2093 | 1322 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1473.56 | 1700.22 | 2693.42 | 2054.69 | 1579.01 | 1736.03 | 1954.72 | 2008.67 | 1516.97 | 1716.87 | 2102.60 | 2044.70 |
Standard Deviation | 237.60 | 332.97 | 382.19 | 358.62 | 270.78 | 321.83 | 330.88 | 409.92 | 257.08 | 328.33 | 451.86 | 370.84 |
Intensity Change Log 2 | — | 0.206416 | 0.870133 | 0.479615 | — | 0.136772 | 0.307942 | 0.347220 | — | 0.170811 | 0.606629 | 0.412649 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000081 | 0.000123 | 0.000464 | 0.000486 | 0.000154 | 0.000442 | 0.000177 | 0.000568 | 0.000111 | 0.000271 | 0.000235 | 0.000504 |
Bud Neck | 0.000127 | 0.000169 | 0.007593 | 0.001819 | 0.000229 | 0.000537 | 0.002588 | 0.002496 | 0.000169 | 0.000340 | 0.003590 | 0.001966 |
Bud Site | 0.008468 | 0.009654 | 0.014468 | 0.023748 | 0.009126 | 0.017168 | 0.012834 | 0.053126 | 0.008739 | 0.013147 | 0.013161 | 0.030126 |
Cell Periphery | 0.000303 | 0.000707 | 0.007327 | 0.011604 | 0.000944 | 0.000891 | 0.000475 | 0.007969 | 0.000567 | 0.000792 | 0.001847 | 0.010815 |
Cytoplasm | 0.923382* | 0.888316* | 0.499623* | 0.790929* | 0.878227* | 0.852011* | 0.623128* | 0.654618* | 0.904795* | 0.871438* | 0.598403* | 0.761336* |
Cytoplasmic Foci | 0.033909 | 0.036778 | 0.042236 | 0.013906 | 0.044251 | 0.056169 | 0.018698 | 0.035209 | 0.038166 | 0.045793 | 0.023410 | 0.018531 |
Eisosomes | 0.000001 | 0.000001 | 0.000005 | 0.000005 | 0.000001 | 0.000003 | 0.000002 | 0.000005 | 0.000001 | 0.000002 | 0.000003 | 0.000005 |
Endoplasmic Reticulum | 0.000987 | 0.002062 | 0.000647 | 0.000572 | 0.003581 | 0.002609 | 0.002712 | 0.001119 | 0.002055 | 0.002316 | 0.002298 | 0.000691 |
Endosome | 0.001185 | 0.002312 | 0.006529 | 0.002748 | 0.002754 | 0.006385 | 0.007736 | 0.008155 | 0.001831 | 0.004206 | 0.007494 | 0.003922 |
Golgi | 0.010593 | 0.024744 | 0.087616 | 0.026727 | 0.026368 | 0.032643 | 0.013491 | 0.046120 | 0.017086 | 0.028416 | 0.028330 | 0.030937 |
Lipid Particles | 0.000511 | 0.001071 | 0.014474 | 0.021522 | 0.001449 | 0.002354 | 0.002498 | 0.016772 | 0.000897 | 0.001667 | 0.004896 | 0.020491 |
Mitochondria | 0.000416 | 0.000161 | 0.000137 | 0.000428 | 0.000087 | 0.000205 | 0.000166 | 0.000111 | 0.000280 | 0.000181 | 0.000161 | 0.000359 |
Mitotic Spindle | 0.000375 | 0.002807 | 0.016130 | 0.001054 | 0.000784 | 0.001804 | 0.004164 | 0.004480 | 0.000544 | 0.002341 | 0.006559 | 0.001798 |
None | 0.000538 | 0.000723 | 0.000737 | 0.000441 | 0.000311 | 0.000376 | 0.000723 | 0.000329 | 0.000445 | 0.000562 | 0.000726 | 0.000416 |
Nuclear Periphery | 0.000012 | 0.000030 | 0.001319 | 0.000086 | 0.000055 | 0.000048 | 0.000519 | 0.000082 | 0.000029 | 0.000038 | 0.000679 | 0.000085 |
Nuclear Periphery Foci | 0.000661 | 0.001593 | 0.023561 | 0.004115 | 0.002193 | 0.002404 | 0.006500 | 0.004436 | 0.001292 | 0.001970 | 0.009915 | 0.004185 |
Nucleolus | 0.000026 | 0.000019 | 0.000177 | 0.000099 | 0.000016 | 0.000099 | 0.000216 | 0.000098 | 0.000022 | 0.000056 | 0.000208 | 0.000099 |
Nucleus | 0.001303 | 0.001501 | 0.010183 | 0.005277 | 0.001629 | 0.001673 | 0.014357 | 0.003200 | 0.001437 | 0.001581 | 0.013521 | 0.004826 |
Peroxisomes | 0.000068 | 0.000063 | 0.000071 | 0.000279 | 0.000056 | 0.000201 | 0.000104 | 0.000436 | 0.000063 | 0.000127 | 0.000098 | 0.000313 |
Vacuole | 0.006094 | 0.009880 | 0.191433 | 0.079735 | 0.010134 | 0.011039 | 0.247012* | 0.128963 | 0.007757 | 0.010419 | 0.235885* | 0.090422 |
Vacuole Periphery | 0.010961 | 0.017288 | 0.075270 | 0.014420 | 0.017650 | 0.010940 | 0.041900 | 0.031707 | 0.013714 | 0.014337 | 0.048580 | 0.018173 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.65 | -4.50 | -11.40 | -9.85 | -0.04 | -4.05 | -1.92 | -4.63 | -1.06 | -4.13 | -4.69 | -6.16 | -11.57 | -5.19 | -6.30 |
Bud Neck | -1.13 | -10.81 | -12.97 | -12.95 | 8.59 | -3.51 | -10.55 | -8.78 | -7.32 | 1.39 | -3.73 | -14.81 | -15.51 | -13.57 | 7.53 |
Bud Site | -1.09 | -4.72 | -7.60 | -6.92 | -3.71 | -5.60 | -3.73 | -6.49 | -5.17 | -5.70 | -4.92 | -5.24 | -9.93 | -7.61 | -6.93 |
Cell Periphery | -4.28 | -9.67 | -11.98 | -11.48 | -3.54 | 0.35 | 3.63 | -5.73 | -5.80 | -6.17 | -2.85 | -7.60 | -12.99 | -12.68 | -11.14 |
Cytoplasm | 5.31 | 25.45 | 16.20 | 10.50 | -16.43 | 2.94 | 27.52 | 11.74 | 10.09 | -1.91 | 6.16 | 40.58 | 18.34 | 13.03 | -16.64 |
Cytoplasmic Foci | -1.02 | -3.14 | 10.13 | 9.59 | 9.06 | -3.09 | 9.84 | 2.10 | 4.48 | -4.32 | -3.27 | 7.89 | 10.92 | 12.74 | 4.04 |
Eisosomes | -0.54 | -6.35 | -8.79 | -8.65 | 1.59 | -3.87 | -7.46 | -5.54 | -2.57 | -3.58 | -3.95 | -10.75 | -10.14 | -6.71 | -3.51 |
Endoplasmic Reticulum | -2.17 | 2.06 | 2.66 | 3.16 | 0.61 | 1.06 | 1.09 | 3.17 | 2.90 | 2.93 | -0.55 | -0.29 | 4.12 | 4.75 | 3.91 |
Endosome | -4.98 | -11.66 | -3.18 | -0.78 | 6.23 | -4.95 | -9.93 | -5.21 | -1.31 | -0.15 | -6.55 | -14.66 | -4.58 | 0.64 | 6.59 |
Golgi | -7.29 | -16.76 | -7.33 | -0.48 | 12.15 | -2.43 | 7.66 | -3.56 | -2.16 | -6.61 | -7.23 | -6.88 | -6.33 | -0.61 | -1.16 |
Lipid Particles | -3.77 | -10.70 | -12.38 | -12.03 | -2.92 | -3.10 | -5.86 | -5.42 | -5.08 | -4.92 | -4.91 | -13.03 | -13.38 | -12.80 | -10.12 |
Mitochondria | 1.01 | 1.06 | 0.02 | -0.85 | -0.89 | -2.00 | -4.38 | -0.65 | 1.59 | 2.19 | 0.65 | 0.78 | -0.23 | -0.70 | -0.78 |
Mitotic Spindle | -2.64 | -7.84 | -3.27 | 1.85 | 7.51 | -3.43 | -6.64 | -3.43 | -2.41 | 0.18 | -3.52 | -10.34 | -4.37 | 0.91 | 7.78 |
None | -0.93 | -2.84 | 1.34 | 1.43 | 4.10 | -0.94 | -2.33 | -0.07 | 0.63 | 2.13 | -1.05 | -1.91 | 0.59 | 1.29 | 2.09 |
Nuclear Periphery | -1.90 | -12.28 | -6.82 | -4.08 | 11.59 | 0.22 | -5.88 | -0.98 | -2.13 | 5.88 | -0.54 | -10.19 | -3.76 | -3.92 | 9.44 |
Nuclear Periphery Foci | -4.21 | -13.88 | -13.01 | -8.13 | 11.70 | -0.59 | -8.15 | -4.25 | -3.54 | 3.55 | -3.25 | -15.77 | -11.84 | -7.92 | 10.14 |
Nucleolus | 1.26 | -8.20 | -8.98 | -10.34 | 4.61 | -1.40 | -13.37 | -8.75 | 0.04 | 7.29 | -1.25 | -14.84 | -12.11 | -1.41 | 8.72 |
Nucleus | -1.43 | -9.00 | -7.74 | -7.37 | 4.31 | -0.11 | -11.60 | -2.82 | -3.34 | 9.25 | -0.64 | -14.30 | -7.71 | -7.89 | 8.88 |
Peroxisomes | 0.45 | -1.05 | -6.34 | -6.50 | -5.97 | -3.05 | -6.04 | -7.15 | -3.26 | -6.08 | -2.81 | -5.27 | -8.88 | -5.07 | -7.29 |
Vacuole | -2.86 | -17.98 | -19.08 | -17.59 | 10.43 | -0.61 | -37.13 | -10.51 | -10.42 | 8.56 | -2.65 | -41.64 | -20.92 | -20.04 | 21.50 |
Vacuole Periphery | -3.13 | -11.04 | -1.83 | 1.59 | 10.43 | 3.40 | -8.24 | -2.78 | -4.57 | 2.04 | -0.44 | -14.90 | -2.28 | -1.85 | 12.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|