Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 31 | 13 | 34 | 51 | 28 | 27 | 51 | 18 | 41 | 31 | 34 | 77 | 58 | 48 | 9 | 7 | 4 | 10 | 20 | 4 | 23 | 37 | 51 |
Bud | 3 | 2 | 1 | 2 | 4 | 7 | 5 | 12 | 14 | 11 | 26 | 14 | 11 | 9 | 9 | 2 | 1 | 1 | 0 | 3 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 0 | 1 | 4 | 0 | 3 | 4 | 2 | 0 | 4 | 5 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 13 | 3 | 16 | 19 | 19 | 23 | 20 | 11 | 19 | 16 | 20 | 8 | 29 | 28 | 1 | 4 | 2 | 0 | 1 | 0 | 1 | 2 | 8 |
Endoplasmic Reticulum | 1 | 13 | 2 | 10 | 6 | 7 | 8 | 11 | 8 | 10 | 2 | 3 | 16 | 28 | 15 | 2 | 7 | 1 | 0 | 1 | 0 | 0 | 4 | 5 |
Endosome | 29 | 35 | 25 | 70 | 95 | 74 | 151 | 136 | 84 | 83 | 108 | 81 | 91 | 116 | 252 | 10 | 34 | 10 | 39 | 65 | 31 | 99 | 165 | 109 |
Golgi | 39 | 80 | 37 | 76 | 144 | 42 | 93 | 82 | 49 | 73 | 38 | 52 | 135 | 240 | 261 | 23 | 32 | 20 | 55 | 86 | 41 | 165 | 273 | 263 |
Mitochondria | 35 | 55 | 2 | 19 | 15 | 124 | 47 | 94 | 137 | 107 | 150 | 127 | 51 | 86 | 108 | 6 | 12 | 3 | 2 | 11 | 3 | 5 | 7 | 10 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 0 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 2 | 2 | 3 | 4 | 9 | 21 | 23 | 19 | 20 | 18 | 17 | 5 | 7 | 17 | 5 | 5 | 1 | 0 | 0 | 0 | 1 | 1 | 2 |
Peroxisomes | 1 | 1 | 1 | 3 | 0 | 4 | 11 | 13 | 7 | 4 | 26 | 15 | 22 | 13 | 16 | 0 | 0 | 0 | 16 | 24 | 14 | 46 | 60 | 39 |
SpindlePole | 3 | 3 | 6 | 18 | 39 | 10 | 61 | 53 | 30 | 44 | 33 | 39 | 17 | 9 | 22 | 4 | 14 | 3 | 3 | 6 | 1 | 7 | 10 | 2 |
Vac/Vac Membrane | 8 | 22 | 6 | 13 | 27 | 13 | 40 | 26 | 19 | 19 | 21 | 10 | 9 | 15 | 28 | 4 | 10 | 1 | 1 | 3 | 0 | 2 | 2 | 2 |
Unique Cell Count | 94 | 178 | 69 | 201 | 315 | 240 | 367 | 394 | 300 | 320 | 355 | 325 | 305 | 454 | 613 | 43 | 84 | 34 | 130 | 229 | 98 | 357 | 574 | 506 |
Labelled Cell Count | 135 | 261 | 98 | 266 | 409 | 339 | 493 | 529 | 401 | 435 | 476 | 422 | 445 | 618 | 804 | 68 | 129 | 47 | 130 | 229 | 98 | 357 | 574 | 506 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 9.6 | 9.8 | 9.5 | 10.2 | 7.6 | 9.4 | 8.9 | 8.3 | 8.8 | 8.8 | 8.9 | 9.9 | 9.9 | 9.8 | 11.2 | 10.1 | 11.0 | 8.9 | 8.8 | 9.0 |
Std Deviation (1e-4) | 1.3 | 1.2 | 1.2 | 1.2 | 1.5 | 1.8 | 1.6 | 1.4 | 1.5 | 1.3 | 1.5 | 1.5 | 1.5 | 1.4 | 1.4 | 1.5 | 2.1 | 1.7 | 1.7 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.06 | -0.37 | -0.06 | -0.13 | -0.23 | -0.16 | -0.16 | -0.14 | 0.01 | 0.02 | 0.01 | 0.2 | 0.05 | 0.18 | -0.13 | -0.15 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | -0.5 | -1.5 | -3.0 | -1.3 | -3.5 | -1.3 | -2.5 | -2.0 | 1.1 | -1.4 | -3.0 | 0.3 | -1.9 | -0.9 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.2 | -1.0 | -0.8 | 0.8 | -0.3 | -1.4 | -1.7 | -1.0 | -1.9 | -1.0 | -1.9 | 0.8 | -1.4 | 0.5 | -0.7 |
Golgi | -2.3 | -1.2 | -6.1 | -4.7 | -5.8 | -6.6 | -5.2 | -8.5 | -6.8 | -1.4 | -0.1 | -1.8 | 0 | -1.9 | 0.5 |
Mitochondria | 1.8 | 0 | 7.3 | 2.4 | 4.0 | 6.6 | 5.1 | 6.2 | 5.8 | 3.0 | 3.3 | 3.2 | 0 | 2.4 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.1 | 0.9 | 0 | 1.7 | 1.1 | 0.3 | 1.1 | 0.2 | 0.8 | 0 | 0 | 0 | 0 | 1.5 | 0 |
Vacuole | 0 | 0 | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1956 | -1.5821 | -1.1946 | -2.1595 | 0.365 | -1.1678 | 0.1955 | -0.5658 | -0.3668 | -0.4637 | -0.411 | -0.7501 | 2.1589 | 1.2406 | 1.4271 | 0.4715 | 0.6198 | 0.8326 |
Actin | 0.1071 | 0.0005 | 0.0244 | 0.0003 | 0.0084 | 0.0106 | 0.0601 | 0.0001 | 0.0087 | 0.0012 | 0.0206 | 0.0003 | 0.0738 | 0.0017 | 0.0366 | 0.0001 | 0 | 0.0203 |
Bud | 0.0045 | 0.0046 | 0.0015 | 0.0056 | 0.0008 | 0.002 | 0.0005 | 0.0007 | 0.0047 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0065 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0048 | 0.0002 | 0.0017 | 0.0001 | 0.0006 | 0.0033 | 0.0057 | 0 | 0.0029 | 0.0001 | 0.0001 | 0.0003 | 0.006 | 0.0007 | 0.0005 | 0 | 0 | 0 |
Bud Periphery | 0.0147 | 0.0051 | 0.0031 | 0.0064 | 0.0007 | 0.0029 | 0.0008 | 0.0005 | 0.0044 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.01 | 0.0001 | 0 | 0.0007 |
Bud Site | 0.0431 | 0.0053 | 0.0313 | 0.0004 | 0.0031 | 0.0135 | 0.0023 | 0.0003 | 0.0251 | 0.0002 | 0.0005 | 0 | 0.0058 | 0.0011 | 0.0229 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0028 | 0.0003 | 0.0022 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0009 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0179 | 0.0153 | 0.0247 | 0.0106 | 0.0109 | 0.0145 | 0.0233 | 0.0574 | 0.0602 | 0.1217 | 0.1282 | 0.1174 | 0.0058 | 0.0255 | 0.0249 | 0.0792 | 0.0237 | 0.0275 |
Cytoplasmic Foci | 0.0748 | 0.0058 | 0.0099 | 0.0069 | 0.1491 | 0.0036 | 0.0411 | 0.0051 | 0.0186 | 0.0292 | 0.0241 | 0.008 | 0.0125 | 0.0066 | 0.0035 | 0.0045 | 0.0015 | 0.0025 |
Eisosomes | 0.0012 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.001 | 0.0005 | 0 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0051 | 0.0004 | 0.0006 | 0.0004 | 0.004 | 0.0008 | 0.0085 | 0.0013 | 0.001 | 0.0019 | 0.001 | 0.0014 | 0.0011 | 0.0022 | 0.0012 | 0.0009 | 0.0006 | 0.0002 |
Endosome | 0.0521 | 0.0002 | 0.0045 | 0.0002 | 0.1203 | 0.0064 | 0.0255 | 0.0014 | 0.0043 | 0.0081 | 0.0014 | 0.0024 | 0.0111 | 0.0069 | 0.001 | 0.0016 | 0.0004 | 0.0004 |
Golgi | 0.0218 | 0.0001 | 0.0036 | 0.0001 | 0.0287 | 0.0049 | 0.0088 | 0.0001 | 0.0046 | 0.0099 | 0.0055 | 0.0002 | 0.0117 | 0.0067 | 0.0004 | 0.0001 | 0 | 0.0008 |
Lipid Particles | 0.0342 | 0.0005 | 0.022 | 0.0005 | 0.073 | 0.002 | 0.0178 | 0.0015 | 0.0056 | 0.0712 | 0.0084 | 0.0006 | 0.0257 | 0.0014 | 0.0013 | 0.0002 | 0.0002 | 0.001 |
Mitochondria | 0.0203 | 0.0009 | 0.0074 | 0.0009 | 0.0171 | 0.0047 | 0.0033 | 0.0006 | 0.0014 | 0.0037 | 0.0027 | 0.0003 | 0.0143 | 0.0013 | 0.0005 | 0.0003 | 0.0001 | 0.0023 |
None | 0.4977 | 0.9577 | 0.8568 | 0.9559 | 0.4893 | 0.9179 | 0.7054 | 0.9281 | 0.8391 | 0.6205 | 0.5342 | 0.8651 | 0.7958 | 0.9407 | 0.8837 | 0.9107 | 0.9726 | 0.9406 |
Nuclear Periphery | 0.0044 | 0.0001 | 0.0003 | 0.0009 | 0.0268 | 0.0016 | 0.0268 | 0.0003 | 0.001 | 0.0009 | 0.0001 | 0.0003 | 0.0011 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Nucleolus | 0.0118 | 0.0004 | 0.0003 | 0.0009 | 0.0029 | 0.002 | 0.0019 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0003 | 0.0007 | 0.0004 | 0.0009 | 0.0001 | 0 | 0 |
Nucleus | 0.0034 | 0.0006 | 0.0004 | 0.0013 | 0.0117 | 0.0032 | 0.0216 | 0.0004 | 0.0025 | 0.0005 | 0.0001 | 0.0009 | 0.0003 | 0.0008 | 0.0008 | 0.0003 | 0.0001 | 0 |
Peroxisomes | 0.0417 | 0.0006 | 0.0015 | 0.0008 | 0.0186 | 0.0011 | 0.0245 | 0.0001 | 0.0096 | 0.021 | 0.2717 | 0.0001 | 0.0279 | 0.0004 | 0.001 | 0.0001 | 0 | 0.0026 |
Punctate Nuclear | 0.0246 | 0.0005 | 0.0005 | 0.0068 | 0.0262 | 0.0022 | 0.0169 | 0.0002 | 0.0037 | 0.108 | 0.0002 | 0.001 | 0.0025 | 0.0008 | 0.001 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0102 | 0.0004 | 0.0027 | 0.0006 | 0.0044 | 0.0016 | 0.002 | 0.0011 | 0.0009 | 0.0009 | 0.0006 | 0.001 | 0.0009 | 0.0006 | 0.0015 | 0.0008 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0019 | 0 | 0.0003 | 0.0002 | 0.0033 | 0.0005 | 0.0017 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.2693 | 24.6594 | 29.1418 | 21.3866 | 21.8566 | 27.5698 | 25.8565 | 22.1259 | 22.2857 | 24.3884 |
Translational Efficiency | 0.8112 | 1.161 | 0.8026 | 1.09 | 1.0806 | 0.7733 | 0.8303 | 0.7609 | 0.667 | 0.9031 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
404 | 639 | 1822 | 490 | 187 | 1759 | 1461 | 209 | 591 | 2398 | 3283 | 699 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 569.59 | 679.64 | 796.54 | 829.23 | 597.14 | 713.11 | 785.02 | 913.70 | 578.31 | 704.19 | 791.41 | 854.49 |
Standard Deviation | 67.80 | 80.91 | 116.58 | 106.93 | 63.54 | 93.24 | 101.38 | 124.10 | 67.70 | 91.33 | 110.22 | 118.81 |
Intensity Change Log 2 | — | 0.254847 | 0.483823 | 0.541848 | — | 0.256055 | 0.394660 | 0.613651 | — | 0.255466 | 0.438878 | 0.579044 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000177 | 0.000604 | 0.000443 | 0.000505 | 0.000298 | 0.000488 | 0.000297 | 0.004106 | 0.000215 | 0.000519 | 0.000378 | 0.001582 |
Bud Neck | 0.026065 | 0.049584 | 0.014478 | 0.008362 | 0.028218 | 0.058093 | 0.006817 | 0.011434 | 0.026746 | 0.055826 | 0.011069 | 0.009281 |
Bud Site | 0.003520 | 0.021037 | 0.018095 | 0.021891 | 0.006029 | 0.029293 | 0.012731 | 0.035192 | 0.004314 | 0.027093 | 0.015708 | 0.025868 |
Cell Periphery | 0.000451 | 0.000182 | 0.000205 | 0.000280 | 0.000274 | 0.000436 | 0.000188 | 0.000313 | 0.000395 | 0.000368 | 0.000197 | 0.000290 |
Cytoplasm | 0.342522* | 0.378641* | 0.293081* | 0.289822* | 0.332438* | 0.378650* | 0.362471* | 0.233828* | 0.339332* | 0.378648* | 0.323961* | 0.273080* |
Cytoplasmic Foci | 0.300162* | 0.257992* | 0.004196 | 0.025214 | 0.314692* | 0.201899* | 0.016628 | 0.042913 | 0.304759* | 0.216846* | 0.009728 | 0.030506 |
Eisosomes | 0.000582 | 0.000182 | 0.000027 | 0.000110 | 0.000423 | 0.000219 | 0.000050 | 0.000097 | 0.000532 | 0.000209 | 0.000037 | 0.000106 |
Endoplasmic Reticulum | 0.000864 | 0.000761 | 0.003828 | 0.008952 | 0.000458 | 0.000741 | 0.003510 | 0.010413 | 0.000735 | 0.000747 | 0.003686 | 0.009389 |
Endosome | 0.010531 | 0.018542 | 0.001595 | 0.007828 | 0.007979 | 0.019616 | 0.002929 | 0.021065 | 0.009723 | 0.019330 | 0.002189 | 0.011786 |
Golgi | 0.005216 | 0.004562 | 0.000789 | 0.008181 | 0.001832 | 0.007253 | 0.000659 | 0.006105 | 0.004145 | 0.006536 | 0.000731 | 0.007560 |
Lipid Particles | 0.026341 | 0.005962 | 0.000734 | 0.003899 | 0.014353 | 0.008015 | 0.001658 | 0.004009 | 0.022548 | 0.007468 | 0.001145 | 0.003932 |
Mitochondria | 0.025425 | 0.012800 | 0.001961 | 0.001432 | 0.004275 | 0.021564 | 0.000957 | 0.021765 | 0.018732 | 0.019229 | 0.001514 | 0.007512 |
Mitotic Spindle | 0.000312 | 0.002485 | 0.013922 | 0.037547 | 0.001060 | 0.007213 | 0.009927 | 0.051634 | 0.000549 | 0.005953 | 0.012144 | 0.041759 |
None | 0.004625 | 0.003780 | 0.008989 | 0.005603 | 0.001466 | 0.007319 | 0.004578 | 0.003005 | 0.003626 | 0.006376 | 0.007026 | 0.004826 |
Nuclear Periphery | 0.000453 | 0.000347 | 0.002333 | 0.003218 | 0.000356 | 0.000428 | 0.002302 | 0.002746 | 0.000422 | 0.000407 | 0.002319 | 0.003077 |
Nuclear Periphery Foci | 0.000199 | 0.000311 | 0.000648 | 0.002865 | 0.000143 | 0.000196 | 0.000984 | 0.003954 | 0.000181 | 0.000227 | 0.000798 | 0.003190 |
Nucleolus | 0.002187 | 0.001938 | 0.000521 | 0.001465 | 0.000996 | 0.002749 | 0.000525 | 0.000757 | 0.001810 | 0.002533 | 0.000523 | 0.001253 |
Nucleus | 0.205816* | 0.148299* | 0.599380* | 0.366028* | 0.222164* | 0.173206* | 0.503994* | 0.321593* | 0.210989* | 0.166569* | 0.556931* | 0.352742* |
Peroxisomes | 0.022141 | 0.026255 | 0.000274 | 0.000721 | 0.026124 | 0.014805 | 0.000446 | 0.002193 | 0.023401 | 0.017856 | 0.000350 | 0.001161 |
Vacuole | 0.021275 | 0.063942 | 0.034016 | 0.195547 | 0.035925 | 0.064686 | 0.067006 | 0.203404* | 0.025910 | 0.064487 | 0.048697 | 0.197896 |
Vacuole Periphery | 0.001137 | 0.001794 | 0.000486 | 0.010530 | 0.000500 | 0.003131 | 0.001345 | 0.019476 | 0.000935 | 0.002775 | 0.000868 | 0.013205 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.61 | -6.73 | -8.32 | -1.11 | -2.28 | -1.21 | -0.56 | -2.15 | -1.95 | -2.10 | -3.02 | -5.39 | -3.49 | -2.35 | -2.61 |
Bud Neck | -3.78 | 2.75 | 3.85 | 8.23 | 2.70 | -5.20 | 4.00 | 3.01 | 14.57 | -3.29 | -7.36 | 4.70 | 4.86 | 17.55 | 0.40 |
Bud Site | -7.91 | -6.27 | -3.75 | 2.08 | 0.34 | -7.49 | -1.29 | -3.49 | 1.26 | -2.93 | -11.63 | -5.75 | -5.06 | 3.64 | -1.61 |
Cell Periphery | 5.50 | 5.39 | 4.43 | -3.04 | -2.74 | -2.43 | 3.51 | 0.71 | 2.91 | -3.31 | 0.48 | 6.18 | 4.42 | 2.86 | -4.35 |
Cytoplasm | -2.02 | 7.34 | 9.35 | 12.29 | 4.39 | -2.47 | 2.03 | 6.87 | 13.16 | 7.51 | -3.33 | 6.69 | 11.54 | 18.95 | 8.28 |
Cytoplasmic Foci | 2.96 | 25.71 | 24.33 | 26.99 | -7.92 | 6.05 | 16.51 | 15.76 | 29.67 | -2.45 | 8.31 | 30.36 | 28.96 | 43.24 | -6.36 |
Eisosomes | 3.42 | 4.67 | 3.84 | 3.87 | -16.73 | 3.95 | 7.11 | 5.91 | 6.70 | -7.44 | 3.96 | 5.95 | 4.94 | 7.38 | -17.31 |
Endoplasmic Reticulum | 0.45 | -17.68 | -17.40 | -17.63 | -8.95 | -1.80 | -19.58 | -9.67 | -9.43 | -5.38 | -0.12 | -24.91 | -19.58 | -19.62 | -10.47 |
Endosome | -3.20 | 4.97 | 2.47 | 6.86 | -8.13 | -6.42 | 3.74 | -0.90 | 4.12 | -3.68 | -5.91 | 5.81 | 1.72 | 10.01 | -7.14 |
Golgi | 0.72 | 5.03 | 1.29 | 1.00 | -0.97 | -5.71 | 3.96 | 0.05 | 3.52 | -1.09 | -2.56 | 5.70 | 1.22 | 2.69 | -1.24 |
Lipid Particles | 7.34 | 9.35 | 8.31 | 3.78 | -12.63 | 2.06 | 4.08 | 3.34 | 4.92 | -5.09 | 6.98 | 10.07 | 8.86 | 6.53 | -12.23 |
Mitochondria | 2.73 | 5.34 | 5.54 | 6.33 | 3.97 | -7.82 | 2.76 | -0.44 | 3.20 | -1.23 | -0.07 | 5.64 | 4.86 | 8.52 | -0.84 |
Mitotic Spindle | -3.99 | -6.89 | -4.65 | -4.24 | -2.36 | -4.19 | -4.70 | -4.45 | -3.95 | -3.74 | -5.63 | -8.52 | -6.41 | -5.35 | -4.35 |
None | 0.79 | -2.08 | 1.77 | 0.70 | 5.46 | -4.33 | -3.94 | -1.81 | 3.01 | 1.19 | -2.40 | -2.68 | 0.91 | 3.30 | 5.05 |
Nuclear Periphery | 1.99 | -23.34 | -15.69 | -16.11 | -4.84 | -1.01 | -23.62 | -11.43 | -11.08 | -1.68 | 0.16 | -32.96 | -19.16 | -18.91 | -5.00 |
Nuclear Periphery Foci | -3.22 | -9.51 | -9.25 | -8.77 | -6.60 | -1.71 | -7.36 | -5.69 | -5.61 | -3.67 | -2.09 | -10.71 | -10.44 | -10.25 | -6.75 |
Nucleolus | 0.33 | 2.74 | 1.97 | 3.03 | -2.39 | -3.41 | 1.89 | 0.54 | 3.74 | -1.94 | -1.55 | 3.00 | 2.02 | 4.08 | -2.91 |
Nucleus | 4.30 | -32.73 | -10.51 | -15.42 | 15.68 | 2.69 | -16.11 | -3.85 | -7.21 | 9.36 | 4.35 | -35.04 | -10.86 | -16.73 | 17.07 |
Peroxisomes | -1.03 | 7.35 | 7.25 | 10.10 | -4.26 | 1.97 | 4.56 | 4.40 | 13.62 | -3.39 | 1.93 | 8.53 | 8.37 | 17.29 | -4.96 |
Vacuole | -9.19 | -10.96 | -22.09 | -18.21 | -18.51 | -3.92 | -10.32 | -13.84 | -13.10 | -10.07 | -10.94 | -16.32 | -26.00 | -22.90 | -20.24 |
Vacuole Periphery | -2.45 | 4.68 | -1.29 | -0.89 | -1.80 | -5.22 | -0.60 | -1.65 | -0.75 | -1.54 | -4.84 | 1.80 | -2.04 | -0.73 | -2.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|