Standard name | |
---|---|
Human Ortholog | |
Description | Widely-conserved NADHX dehydratase; converts (S)-NADHX to NADH in ATP-dependent manner; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; downstream intergenic region drives antisense expression and mediates coordinated regulation of YKL151C and GPM1 phosphoglycerate mutase; protein abundance increases in response to DNA replication stress; homolog of Carkd in mammals and C-terminus of YjeF in E.coli |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 2 |
Bud | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 5 | 0 | 11 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 4 | 0 | 4 | 6 | 4 | 3 | 1 | 3 | 5 | 0 | 0 | 3 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 283 | 129 | 153 | 252 | 376 | 269 | 284 | 307 | 147 | 101 | 167 | 141 | 225 | 277 | 330 | 95 | 76 | 94 | 275 | 124 | 158 | 143 | 265 | 293 |
Endoplasmic Reticulum | 3 | 1 | 3 | 0 | 0 | 1 | 0 | 6 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 10 | 0 | 0 | 10 | 11 | 41 | 80 | 72 | 81 | 42 | 123 | 72 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 3 | 1 | 2 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 3 | 7 | 5 | 8 | 6 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 2 | 1 | 1 | 5 | 12 | 12 | 24 | 43 | 27 | 21 | 25 | 36 | 3 | 8 | 3 | 5 | 2 | 1 | 1 | 1 | 0 | 0 | 2 | 2 |
Unique Cell Count | 292 | 131 | 157 | 259 | 390 | 299 | 354 | 396 | 226 | 153 | 273 | 236 | 228 | 284 | 336 | 100 | 78 | 96 | 296 | 132 | 170 | 153 | 292 | 328 |
Labelled Cell Count | 299 | 131 | 159 | 274 | 401 | 332 | 406 | 444 | 277 | 174 | 339 | 273 | 231 | 287 | 339 | 108 | 83 | 104 | 296 | 132 | 170 | 153 | 292 | 328 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 6.9 | 6.0 | 5.9 | 6.1 | 5.4 | 5.4 | 6.2 | 5.6 | 5.6 | 5.2 | 5.9 | 6.0 | 7.6 | 8.3 | 14.5 | 15.6 | 14.2 | 5.6 | 5.8 | 6.8 |
Std Deviation (1e-4) | 0.7 | 1.5 | 1.8 | 2.2 | 1.7 | 1.2 | 1.3 | 1.5 | 2.5 | 1.5 | 1.4 | 1.6 | 1.6 | 1.9 | 2.5 | 2.9 | 3.6 | 2.5 | 1.3 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.05 | -0.14 | -0.14 | 0.06 | -0.09 | -0.1 | -0.2 | -0.01 | 0.02 | 0.36 | 0.48 | 1.28 | 1.39 | 1.26 | -0.09 | -0.04 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | -0.6 | -2.9 | -5.1 | -5.6 | -7.6 | -7.2 | -8.3 | -8.4 | 0.9 | 0.1 | 0.6 | -1.0 | 0 | 0.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 6.5 | 5.7 | 8.4 | 7.1 | 10.0 | 7.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.0 | 4.0 | 4.2 | 4.5 | 3.6 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7453 | 3.9937 | 3.1652 | 2.8083 | 3.1766 | 3.3752 | 1.4499 | 2.0381 | 1.7655 | 2.0605 | 1.7103 | 1.7959 | 3.0006 | 3.7662 | 3.0731 | 3.5213 | 3.6628 | 3.603 |
Actin | 0.0037 | 0.0003 | 0.0041 | 0.0012 | 0.001 | 0.0017 | 0.0132 | 0.0005 | 0.0026 | 0.0007 | 0.0113 | 0.0023 | 0.0359 | 0.0015 | 0.0143 | 0.0631 | 0.0992 | 0.0043 |
Bud | 0.0022 | 0.0006 | 0.0036 | 0.0038 | 0.0002 | 0.0069 | 0.0013 | 0.0044 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0006 | 0.0039 | 0.001 | 0.0036 | 0.0013 | 0.0004 |
Bud Neck | 0.0003 | 0.0003 | 0.0097 | 0.0006 | 0.0006 | 0.0014 | 0.0053 | 0.0002 | 0.0004 | 0.0006 | 0.0011 | 0.0012 | 0.0183 | 0.0002 | 0.0039 | 0.0281 | 0.002 | 0.0029 |
Bud Periphery | 0.0009 | 0.0002 | 0.0013 | 0.0006 | 0.0001 | 0.0027 | 0.0018 | 0.0012 | 0.0004 | 0.0002 | 0.0004 | 0.0007 | 0.0006 | 0.0022 | 0.0008 | 0.0023 | 0.0021 | 0.0002 |
Bud Site | 0.0032 | 0.0044 | 0.005 | 0.0038 | 0.0002 | 0.0006 | 0.0104 | 0.01 | 0.0049 | 0.0002 | 0.0015 | 0.0003 | 0.004 | 0.0089 | 0.0028 | 0.0106 | 0.0019 | 0.0004 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 |
Cytoplasm | 0.8159 | 0.9213 | 0.8261 | 0.8393 | 0.9122 | 0.8664 | 0.6219 | 0.8392 | 0.7681 | 0.8092 | 0.7015 | 0.7389 | 0.7837 | 0.9257 | 0.8913 | 0.72 | 0.6802 | 0.9308 |
Cytoplasmic Foci | 0.0122 | 0.0082 | 0.0122 | 0.0134 | 0.0113 | 0.0207 | 0.0372 | 0.0071 | 0.0125 | 0.0102 | 0.0346 | 0.0201 | 0.0243 | 0.0082 | 0.0099 | 0.047 | 0.0239 | 0.013 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0031 | 0.0025 | 0.0042 | 0.0047 | 0.0035 | 0.0056 | 0.0019 | 0.0031 | 0.0017 | 0.0012 | 0.0044 | 0.0042 | 0.0022 | 0.0039 | 0.0069 | 0.0104 | 0.0023 |
Endosome | 0.0199 | 0.0079 | 0.0056 | 0.0142 | 0.0075 | 0.0114 | 0.0372 | 0.003 | 0.0123 | 0.0028 | 0.0661 | 0.0153 | 0.0165 | 0.0039 | 0.0132 | 0.0451 | 0.0584 | 0.0065 |
Golgi | 0.003 | 0.0005 | 0.0004 | 0.0012 | 0.0007 | 0.0011 | 0.0074 | 0.0002 | 0.0022 | 0.0002 | 0.0256 | 0.0023 | 0.0074 | 0.0004 | 0.0041 | 0.0125 | 0.0156 | 0.0011 |
Lipid Particles | 0.004 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0006 | 0.0085 | 0.0001 | 0.0032 | 0 | 0.0466 | 0.0065 | 0.0073 | 0.0001 | 0.0073 | 0.0045 | 0.0155 | 0.0016 |
Mitochondria | 0.0019 | 0.0005 | 0.0005 | 0.0362 | 0.0004 | 0.0012 | 0.0038 | 0.0002 | 0.0015 | 0.0003 | 0.0292 | 0.0044 | 0.0019 | 0.0006 | 0.0075 | 0.0067 | 0.0105 | 0.0019 |
None | 0.1085 | 0.0405 | 0.1056 | 0.0542 | 0.051 | 0.0558 | 0.2016 | 0.125 | 0.176 | 0.1663 | 0.034 | 0.1821 | 0.0663 | 0.0347 | 0.0195 | 0.0075 | 0.0069 | 0.0217 |
Nuclear Periphery | 0.0041 | 0.0021 | 0.0029 | 0.0023 | 0.0017 | 0.0026 | 0.0137 | 0.0007 | 0.0024 | 0.0011 | 0.0009 | 0.0027 | 0.0028 | 0.0008 | 0.0057 | 0.0097 | 0.0372 | 0.0019 |
Nucleolus | 0.0002 | 0.0001 | 0.0009 | 0.0006 | 0 | 0.0004 | 0.0008 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0029 | 0.0001 | 0.0003 | 0.0004 | 0.0011 | 0.0001 |
Nucleus | 0.0047 | 0.0062 | 0.0104 | 0.0085 | 0.0054 | 0.0092 | 0.0103 | 0.0027 | 0.0032 | 0.0038 | 0.003 | 0.0031 | 0.006 | 0.0035 | 0.0038 | 0.0085 | 0.0127 | 0.0058 |
Peroxisomes | 0.0038 | 0.0001 | 0.0002 | 0.0011 | 0.0009 | 0.0043 | 0.0083 | 0 | 0.0026 | 0.0002 | 0.0357 | 0.0072 | 0.0097 | 0.001 | 0.0037 | 0.0128 | 0.0026 | 0.0011 |
Punctate Nuclear | 0.0015 | 0.0013 | 0.0065 | 0.0115 | 0.0006 | 0.0069 | 0.0057 | 0.0007 | 0.0014 | 0.0006 | 0.0016 | 0.006 | 0.004 | 0.0006 | 0.0042 | 0.0052 | 0.0097 | 0.0027 |
Vacuole | 0.0018 | 0.002 | 0.0017 | 0.0022 | 0.0013 | 0.0018 | 0.0041 | 0.0021 | 0.0014 | 0.0011 | 0.0034 | 0.001 | 0.0025 | 0.0012 | 0.0014 | 0.0029 | 0.005 | 0.001 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0005 | 0.0016 | 0.0002 | 0.0004 | 0.0002 | 0.0014 | 0.0004 | 0.0005 | 0.0002 | 0.001 | 0.0017 | 0.0034 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.6752 | 18.4824 | 17.3239 | 27.6179 | 19.0466 | 39.1524 | 24.343 | 29.2086 | 33.2655 | 27.7114 |
Translational Efficiency | 2.0597 | 1.5772 | 1.7458 | 1.7921 | 1.6074 | 1.3183 | 1.6375 | 1.6047 | 1.7052 | 1.4557 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2382 | 703 | 1561 | 111 | 2292 | 2032 | 447 | 1560 | 4674 | 2735 | 2008 | 1671 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 765.61 | 868.36 | 827.35 | 1164.09 | 743.73 | 812.54 | 1042.98 | 1108.90 | 754.88 | 826.89 | 875.35 | 1112.57 |
Standard Deviation | 91.42 | 111.15 | 179.85 | 179.14 | 95.30 | 100.08 | 120.04 | 191.46 | 93.98 | 105.88 | 190.79 | 191.16 |
Intensity Change Log 2 | — | 0.181684 | 0.111888 | 0.604521 | — | 0.127660 | 0.487861 | 0.576278 | — | 0.155316 | 0.309375 | 0.590674 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000162 | 0.000285 | 0.000292 | 0.002660 | 0.000159 | 0.000297 | 0.000218 | 0.000478 | 0.000161 | 0.000294 | 0.000276 | 0.000623 |
Bud Neck | 0.016021 | 0.017711 | 0.003602 | 0.002495 | 0.009061 | 0.021060 | 0.002415 | 0.004068 | 0.012608 | 0.020199 | 0.003338 | 0.003963 |
Bud Site | 0.005830 | 0.014189 | 0.008491 | 0.028969 | 0.004758 | 0.015754 | 0.007184 | 0.019550 | 0.005305 | 0.015352 | 0.008200 | 0.020175 |
Cell Periphery | 0.000098 | 0.000114 | 0.000128 | 0.000225 | 0.000076 | 0.000091 | 0.000213 | 0.000081 | 0.000087 | 0.000097 | 0.000147 | 0.000091 |
Cytoplasm | 0.556987* | 0.502936* | 0.610565* | 0.521840* | 0.627772* | 0.546740* | 0.474136* | 0.655034* | 0.591698* | 0.535480* | 0.580194* | 0.646186* |
Cytoplasmic Foci | 0.202171* | 0.228811* | 0.008363 | 0.045215 | 0.158442 | 0.180290* | 0.041592 | 0.026155 | 0.180728* | 0.192762* | 0.015760 | 0.027421 |
Eisosomes | 0.000129 | 0.000082 | 0.000018 | 0.000055 | 0.000058 | 0.000073 | 0.000107 | 0.000020 | 0.000094 | 0.000076 | 0.000038 | 0.000022 |
Endoplasmic Reticulum | 0.000888 | 0.000764 | 0.001914 | 0.002895 | 0.000950 | 0.000607 | 0.002319 | 0.000945 | 0.000919 | 0.000648 | 0.002004 | 0.001075 |
Endosome | 0.012263 | 0.025755 | 0.001359 | 0.008385 | 0.006386 | 0.018763 | 0.006980 | 0.009493 | 0.009381 | 0.020560 | 0.002610 | 0.009419 |
Golgi | 0.002944 | 0.004045 | 0.000143 | 0.000533 | 0.001578 | 0.004738 | 0.000118 | 0.009138 | 0.002274 | 0.004560 | 0.000137 | 0.008566 |
Lipid Particles | 0.005175 | 0.003560 | 0.000601 | 0.002598 | 0.002791 | 0.002511 | 0.003009 | 0.000872 | 0.004006 | 0.002780 | 0.001137 | 0.000987 |
Mitochondria | 0.003004 | 0.003220 | 0.000381 | 0.000213 | 0.001401 | 0.005633 | 0.000248 | 0.003508 | 0.002218 | 0.005013 | 0.000352 | 0.003289 |
Mitotic Spindle | 0.002380 | 0.005706 | 0.006603 | 0.005492 | 0.001046 | 0.013186 | 0.000895 | 0.054192 | 0.001726 | 0.011263 | 0.005332 | 0.050957 |
None | 0.002973 | 0.003265 | 0.017999 | 0.001043 | 0.003017 | 0.001471 | 0.006140 | 0.005890 | 0.002995 | 0.001932 | 0.015359 | 0.005568 |
Nuclear Periphery | 0.000237 | 0.000319 | 0.000672 | 0.000655 | 0.000263 | 0.000327 | 0.001113 | 0.000917 | 0.000250 | 0.000325 | 0.000770 | 0.000900 |
Nuclear Periphery Foci | 0.000421 | 0.000885 | 0.002011 | 0.014384 | 0.000425 | 0.000318 | 0.004086 | 0.002046 | 0.000423 | 0.000464 | 0.002473 | 0.002866 |
Nucleolus | 0.000919 | 0.001334 | 0.000469 | 0.000136 | 0.000534 | 0.001144 | 0.000727 | 0.000570 | 0.000730 | 0.001193 | 0.000526 | 0.000541 |
Nucleus | 0.118781 | 0.072204 | 0.297931* | 0.056207 | 0.131389* | 0.087962 | 0.236429* | 0.058350 | 0.124964 | 0.083911 | 0.284240* | 0.058207 |
Peroxisomes | 0.003047 | 0.008747 | 0.000223 | 0.000733 | 0.001807 | 0.005755 | 0.000399 | 0.001357 | 0.002439 | 0.006524 | 0.000262 | 0.001316 |
Vacuole | 0.064576 | 0.104392 | 0.037981 | 0.304765* | 0.047212 | 0.091069 | 0.211394* | 0.142325 | 0.056061 | 0.094494 | 0.076584 | 0.153116 |
Vacuole Periphery | 0.000993 | 0.001674 | 0.000253 | 0.000503 | 0.000874 | 0.002213 | 0.000280 | 0.005012 | 0.000935 | 0.002074 | 0.000259 | 0.004712 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.40 | -4.40 | -1.55 | -1.41 | -1.30 | -1.92 | -2.11 | -1.83 | -0.06 | 0.11 | -3.18 | -4.25 | -2.79 | -1.15 | -0.24 |
Bud Neck | -0.79 | 11.00 | 13.13 | 8.34 | 1.41 | -8.02 | 7.78 | 9.04 | 13.63 | -0.11 | -6.08 | 12.11 | 15.90 | 15.81 | 1.40 |
Bud Site | -5.75 | -1.65 | -2.10 | -0.94 | -1.76 | -7.94 | -0.97 | -5.61 | 2.52 | -2.17 | -9.22 | -2.19 | -5.71 | 2.12 | -2.74 |
Cell Periphery | -1.39 | -3.79 | -4.38 | -3.10 | -1.76 | -2.64 | -10.50 | 3.45 | 4.79 | 11.48 | -1.67 | -8.18 | 4.61 | 5.05 | 10.36 |
Cytoplasm | 4.77 | -0.04 | 5.72 | 3.32 | 5.65 | 10.00 | 14.88 | 5.58 | -3.44 | -10.65 | 8.71 | 8.77 | 3.15 | -3.75 | -4.18 |
Cytoplasmic Foci | -2.57 | 46.67 | 33.34 | 23.01 | -5.31 | -3.67 | 29.03 | 36.42 | 37.04 | 5.49 | -2.22 | 57.84 | 55.16 | 44.34 | -4.74 |
Eisosomes | 5.89 | 16.60 | 10.60 | 4.93 | -6.02 | -3.89 | -6.95 | 19.12 | 15.96 | 13.88 | 4.34 | 13.99 | 21.48 | 18.88 | 10.24 |
Endoplasmic Reticulum | 0.74 | -9.50 | -4.51 | -5.01 | 1.65 | 2.59 | -9.71 | 2.45 | -0.52 | 11.84 | 2.70 | -12.51 | 1.73 | -1.17 | 14.22 |
Endosome | -5.84 | 15.38 | 7.94 | 9.33 | -4.84 | -11.01 | 0.38 | 5.16 | 13.54 | 4.31 | -10.66 | 16.01 | 10.36 | 16.41 | -2.34 |
Golgi | -1.16 | 13.21 | 12.35 | 4.68 | -2.38 | -4.87 | 7.84 | -1.24 | 2.07 | -3.14 | -4.26 | 15.09 | -0.20 | 2.28 | -3.14 |
Lipid Particles | 3.32 | 13.66 | 6.90 | 2.43 | -5.71 | 1.37 | -0.16 | 11.56 | 9.00 | 9.28 | 5.28 | 14.47 | 16.21 | 10.33 | 3.21 |
Mitochondria | -0.14 | 5.44 | 5.73 | 3.59 | 1.75 | -5.35 | 8.90 | 0.64 | 5.03 | -1.99 | -4.12 | 7.33 | 2.43 | 5.30 | -1.69 |
Mitotic Spindle | -1.53 | -2.61 | -1.57 | -0.02 | 0.12 | -6.28 | -0.83 | -9.70 | -4.65 | -9.54 | -6.10 | -3.23 | -9.50 | -5.31 | -7.93 |
None | -0.20 | -6.92 | 2.12 | 1.29 | 7.66 | 4.60 | -1.16 | -2.11 | -5.40 | 0.06 | 2.63 | -7.07 | -2.02 | -3.83 | 5.94 |
Nuclear Periphery | -3.81 | -17.69 | -7.36 | -5.96 | 0.17 | -1.77 | -16.36 | -9.06 | -7.19 | 6.30 | -2.90 | -22.89 | -10.07 | -7.98 | 2.31 |
Nuclear Periphery Foci | -3.16 | -11.43 | -6.74 | -6.38 | -5.31 | 1.58 | -10.30 | -7.77 | -8.72 | 6.93 | -0.88 | -15.10 | -10.02 | -9.69 | 2.67 |
Nucleolus | -1.45 | 4.39 | 7.03 | 4.08 | 4.31 | -3.08 | 0.29 | 2.63 | 4.09 | 2.08 | -2.82 | 4.19 | 4.64 | 5.12 | 1.63 |
Nucleus | 9.68 | -29.17 | 7.10 | 1.02 | 26.83 | 11.06 | -9.49 | 12.01 | 0.60 | 14.01 | 13.81 | -29.99 | 13.29 | -0.06 | 35.92 |
Peroxisomes | -5.37 | 13.31 | 10.36 | 7.99 | -1.28 | -7.51 | 6.44 | 5.18 | 11.28 | -1.12 | -8.82 | 13.83 | 9.93 | 13.61 | -1.96 |
Vacuole | -8.24 | -1.26 | -13.62 | -11.74 | -13.38 | -13.58 | -18.86 | -30.61 | -22.38 | 1.78 | -14.56 | -13.55 | -31.90 | -24.14 | -19.48 |
Vacuole Periphery | -3.22 | 9.34 | 8.20 | 7.15 | -0.27 | -4.35 | 5.05 | -1.41 | 1.43 | -3.05 | -4.99 | 8.97 | -1.18 | 1.48 | -3.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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