Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.95 0.95 0.89 0.86 0.76 0.73 0.74 0.73 0.63 0.6 0.61 0.97 0.96 0.95 0.82 0.93 0.87 0.9 0.81 0.87 0.82 0.73 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0.09 0.11 0.33 0.34 0.3 0.28 0.42 0.49 0.48 0 0 0 0 0 0 0.07 0 0 0.06 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.09 0.07 0.07 0 0 0 0 0.13 0 0.08 0 0.1 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 2 0 0 1 0 1 0 2 0 0 0 0 1 1 0 1 5 0 5 3
Bud 0 1 1 5 8 8 19 9 16 23 18 30 0 0 0 1 1 0 0 0 0 1 7 7
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 4 5
Bud Site 0 0 0 0 1 1 1 0 1 2 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 4 1 3 3 4 14 13 12 17 0 4 5 7 6 6 0 0 0 0 0 0
Cytoplasm 102 96 342 228 262 291 345 327 302 286 245 311 170 253 266 129 145 108 102 102 324 90 167 157
Endoplasmic Reticulum 2 2 2 4 1 1 8 3 0 0 0 5 2 3 4 8 4 4 0 1 6 0 0 3
Endosome 0 0 0 0 4 1 1 2 2 1 0 2 0 0 3 3 0 1 0 0 3 0 5 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0
Mitochondria 13 2 9 22 35 128 160 132 115 192 201 246 0 0 0 4 1 0 8 3 12 6 20 12
Nucleus 0 1 2 0 0 2 2 4 4 2 7 2 0 4 0 10 5 2 0 1 0 1 2 2
Nuclear Periphery 0 1 0 0 0 2 4 3 5 1 6 11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 1
Peroxisomes 0 0 1 2 3 3 0 0 0 0 0 1 1 0 0 1 1 0 0 0 2 0 0 0
SpindlePole 0 0 2 2 1 3 2 5 2 4 3 3 1 1 0 3 1 0 0 0 4 0 3 4
Vac/Vac Membrane 0 0 5 6 10 8 16 24 37 30 30 21 1 6 8 20 4 10 0 12 2 4 8 2
Unique Cell Count 112 101 361 256 306 383 474 443 416 453 407 514 175 264 279 157 156 124 114 126 372 111 228 208
Labelled Cell Count 118 104 368 273 329 452 562 515 498 555 523 654 175 271 287 188 169 133 114 126 372 111 228 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 7.0 5.5 5.1 5.2 4.7 4.6 4.6 4.9 4.4 4.3 4.3 7.3 7.6 7.6 9.2 8.8 8.7 7.7 8.7 9.8
Std Deviation (1e-4) 0.8 1.2 1.1 1.6 1.5 1.8 1.9 1.4 2.1 1.5 1.6 1.4 1.4 1.2 1.9 2.7 2.4 2.0 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.09 -0.08 -0.23 -0.25 -0.26 -0.17 -0.3 -0.36 -0.34 0.41 0.47 0.48 0.75 0.69 0.67 0.49 0.66 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.6 -4.0 -7.2 -8.2 -7.9 -8.2 -10.7 -11.3 -11.5 1.3 0.6 0.3 -4.6 -0.8 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.4 4.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0.7 1.8 3.1 4.6 3.7 4.0 2.3 0 0 1.3 5.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8774 3.931 3.627 3.2113 2.6141 3.6097 2.1078 2.4589 2.2991 2.6217 2.3233 2.4434 3.0247 3.7948 3.2754 2.719 3.6044 3.2854
Actin 0.006 0.0008 0.0031 0.0023 0.0063 0.0028 0.0664 0.0045 0.0057 0.0033 0.0846 0.0142 0.0129 0.0014 0.0042 0.0002 0.0009 0.0017
Bud 0.0058 0.0006 0.0103 0.0209 0.0011 0.0009 0.0009 0.0028 0.0035 0.0012 0.0002 0.0006 0.0003 0.0024 0.004 0.0001 0.0011 0.0005
Bud Neck 0.0006 0.0015 0.0014 0.001 0.001 0.01 0.0188 0.0006 0.0006 0.0009 0.0015 0.0032 0.0015 0.0002 0.0062 0.0005 0.0018 0.0035
Bud Periphery 0.0075 0.0003 0.0209 0.012 0.0016 0.0011 0.0009 0.0015 0.0024 0.0006 0.0002 0.0004 0.0002 0.0006 0.0029 0 0.0005 0.0004
Bud Site 0.0046 0.0168 0.0114 0.0015 0.0003 0.0003 0.0083 0.0148 0.0025 0.0144 0.0004 0.0003 0.001 0.0072 0.0036 0.0001 0.0022 0.0003
Cell Periphery 0.0003 0.0003 0.0004 0.0003 0.0002 0.0002 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0001
Cytoplasm 0.8858 0.8678 0.8429 0.8715 0.8587 0.9055 0.6333 0.8423 0.818 0.8139 0.8212 0.8215 0.8567 0.9377 0.9135 0.903 0.8075 0.9445
Cytoplasmic Foci 0.0068 0.0073 0.0143 0.0098 0.0177 0.012 0.0635 0.0083 0.0176 0.0383 0.0074 0.0185 0.0132 0.0031 0.0164 0.0022 0.0162 0.0053
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0051 0.0059 0.0051 0.0052 0.0038 0.0044 0.0039 0.0033 0.0053 0.0141 0.0043 0.006 0.0093 0.0018 0.0032 0.0029 0.0011 0.0031
Endosome 0.0042 0.0044 0.0078 0.0055 0.0071 0.006 0.0214 0.0016 0.0304 0.0299 0.0044 0.0328 0.0167 0.0007 0.0049 0.0016 0.0346 0.0016
Golgi 0.0007 0.0005 0.0007 0.0004 0.0027 0.0006 0.0108 0.0003 0.0101 0.0064 0.0099 0.0151 0.0028 0.0001 0.0014 0.0001 0.0224 0.0002
Lipid Particles 0.0004 0.0003 0.0007 0.0001 0.0075 0.0002 0.0156 0.0001 0.0014 0.0018 0.0003 0.0036 0.0039 0.0001 0.0008 0 0.0002 0.0002
Mitochondria 0.0002 0.0002 0.0006 0.0003 0.0008 0.0004 0.0048 0.0003 0.015 0.0012 0.0011 0.0015 0.001 0.0002 0.0022 0.0001 0.094 0.0019
None 0.0657 0.0713 0.0598 0.0568 0.0775 0.0453 0.0869 0.1126 0.0705 0.0482 0.0581 0.0601 0.0562 0.0392 0.0204 0.0803 0.0026 0.0302
Nuclear Periphery 0.0012 0.0023 0.0025 0.0017 0.0013 0.0011 0.0056 0.0009 0.0017 0.0092 0.0012 0.007 0.0078 0.0007 0.001 0.002 0.0006 0.001
Nucleolus 0.0001 0.0015 0.0012 0.0001 0 0.0001 0.0012 0.0001 0.0001 0.0002 0 0.0001 0.0005 0.0001 0.0001 0 0 0.0002
Nucleus 0.0022 0.013 0.0089 0.0075 0.0035 0.0056 0.008 0.0032 0.0039 0.0068 0.0031 0.0031 0.0054 0.0024 0.0021 0.0059 0.0057 0.0038
Peroxisomes 0.0002 0.0002 0.0005 0.0002 0.0069 0.0004 0.0197 0.0004 0.0049 0.0037 0.0004 0.001 0.001 0.0001 0.0012 0 0.0003 0.0002
Punctate Nuclear 0.0007 0.0029 0.004 0.0011 0.0011 0.0019 0.0242 0.001 0.0013 0.0027 0.0003 0.0093 0.0076 0.0013 0.0105 0.0004 0.0004 0.0007
Vacuole 0.0017 0.002 0.0029 0.0016 0.001 0.001 0.0042 0.001 0.0038 0.0026 0.0007 0.0012 0.0012 0.0005 0.0011 0.0006 0.0026 0.0005
Vacuole Periphery 0.0002 0.0002 0.0003 0.0003 0.0001 0.0002 0.0007 0.0001 0.0011 0.0005 0.0002 0.0005 0.0005 0.0001 0.0002 0.0001 0.0052 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0197 8.063 4.6535 10.8045 10.395 12.0195 6.5673 7.6422 10.691 9.9636
Translational Efficiency 1.8956 1.3765 2.1885 1.5939 2.0735 2.5718 2.1152 2.3222 2.2343 2.0594

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
602 423 1274 1533 1839 2264 1404 1590 2441 2687 2678 3123

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 701.66 830.17 1033.52 1022.51 759.79 828.24 1074.88 1081.77 745.45 828.54 1055.20 1052.68
Standard Deviation 87.60 105.47 137.80 164.59 103.21 116.17 152.76 173.58 102.69 114.55 147.29 171.80
Intensity Change Log 2 — 0.242635 0.558722 0.543271 — 0.124448 0.500503 0.509721 — 0.182400 0.528748 0.525926

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000224 0.000500 0.000376 0.015358 0.000576 0.000314 0.002509 0.029266 0.000489 0.000344 0.001494 0.022439
Bud Neck 0.019128 0.009854 0.004369 0.017815 0.002954 0.011841 0.005616 0.020435 0.006943 0.011528 0.005023 0.019149
Bud Site 0.006962 0.017112 0.011301 0.199364* 0.003197 0.011086 0.022604 0.258965* 0.004126 0.012034 0.017227 0.229708*
Cell Periphery 0.000191 0.000106 0.000164 0.002655 0.000108 0.000137 0.000589 0.006946 0.000128 0.000132 0.000387 0.004839
Cytoplasm 0.464690* 0.565062* 0.575304* 0.462268* 0.675573* 0.632655* 0.540573* 0.431068* 0.623565* 0.622014* 0.557096* 0.446383*
Cytoplasmic Foci 0.304333* 0.166882 0.012940 0.021653 0.090522 0.118858 0.019245 0.021963 0.143252 0.126418 0.016245 0.021811
Eisosomes 0.000199 0.000078 0.000031 0.000402 0.000039 0.000074 0.000087 0.001068 0.000078 0.000074 0.000060 0.000741
Endoplasmic Reticulum 0.001218 0.001130 0.002485 0.001829 0.001178 0.000588 0.004049 0.002514 0.001188 0.000673 0.003305 0.002178
Endosome 0.020329 0.014494 0.001847 0.005175 0.002380 0.010082 0.002525 0.003298 0.006807 0.010776 0.002202 0.004219
Golgi 0.003563 0.001722 0.000116 0.002131 0.000461 0.002112 0.000938 0.004252 0.001226 0.002050 0.000547 0.003211
Lipid Particles 0.007405 0.001945 0.000934 0.001247 0.001391 0.001780 0.001518 0.001443 0.002874 0.001806 0.001240 0.001347
Mitochondria 0.003346 0.001558 0.000335 0.001173 0.000571 0.002716 0.000349 0.002027 0.001255 0.002533 0.000342 0.001608
Mitotic Spindle 0.000179 0.012090 0.003510 0.021045 0.000098 0.006068 0.004312 0.014367 0.000118 0.007016 0.003931 0.017645
None 0.002448 0.001655 0.003792 0.002002 0.006893 0.002459 0.003412 0.001401 0.005797 0.002332 0.003593 0.001696
Nuclear Periphery 0.000395 0.000440 0.000936 0.000273 0.000237 0.000240 0.000851 0.000172 0.000276 0.000272 0.000891 0.000222
Nuclear Periphery Foci 0.000695 0.001200 0.002311 0.001777 0.000598 0.000535 0.004160 0.001286 0.000622 0.000640 0.003281 0.001527
Nucleolus 0.000657 0.005375 0.000151 0.000101 0.000376 0.000739 0.000133 0.000077 0.000445 0.001469 0.000142 0.000089
Nucleus 0.088848 0.079219 0.286322* 0.080475 0.180595* 0.099940 0.247021* 0.060087 0.157968* 0.096678 0.265718* 0.070095
Peroxisomes 0.005941 0.007523 0.000575 0.002494 0.000563 0.002726 0.000863 0.002992 0.001889 0.003481 0.000726 0.002748
Vacuole 0.068194 0.109494 0.092085 0.158181 0.031344 0.093564 0.138525 0.135970 0.040432 0.096072 0.116432 0.146873
Vacuole Periphery 0.001053 0.002562 0.000117 0.002580 0.000346 0.001488 0.000122 0.000403 0.000521 0.001657 0.000119 0.001472

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.93 -6.05 -7.65 -7.11 -7.34 0.46 -1.30 -8.79 -9.36 -7.80 0.29 -1.19 -10.69 -11.66 -10.11
Bud Neck 3.84 6.78 4.14 -0.19 -5.45 -9.52 -4.05 -9.00 -0.55 -6.67 -4.82 2.41 -5.00 0.18 -8.60
Bud Site -3.29 -2.15 -14.94 -10.56 -13.93 -8.39 -7.42 -21.03 -19.60 -17.98 -8.47 -8.09 -25.85 -23.33 -22.55
Cell Periphery 4.81 1.20 -3.51 -3.91 -3.62 -1.97 -5.08 -4.01 -3.97 -3.55 -0.29 -5.17 -4.86 -4.85 -4.38
Cytoplasm -5.23 -1.11 3.46 8.72 5.74 6.09 26.76 27.96 22.23 1.05 0.85 22.63 27.81 26.72 4.70
Cytoplasmic Foci 10.95 31.54 30.87 16.65 -5.07 -6.65 25.14 22.88 29.43 -3.33 3.74 35.11 33.47 34.69 -5.64
Eisosomes 8.19 12.84 0.38 -3.47 -4.81 -9.78 -8.09 -4.72 -4.38 -4.23 0.87 2.52 -5.13 -5.20 -5.35
Endoplasmic Reticulum 0.31 -8.47 -2.16 -3.22 9.29 2.30 -11.43 -3.31 -10.75 11.10 2.53 -12.91 -3.45 -13.07 14.42
Endosome 2.09 7.76 7.56 6.31 0.02 -10.13 -0.08 1.47 10.14 1.58 -4.15 7.30 6.99 11.65 0.96
Golgi 2.73 5.62 4.46 2.42 -2.25 -4.14 -0.03 -1.60 0.42 -1.44 -2.12 3.77 0.11 1.55 -1.94
Lipid Particles 6.37 7.38 6.86 1.04 -4.63 -1.83 -2.82 -1.48 0.03 0.59 4.04 5.23 4.40 0.44 -1.13
Mitochondria 1.29 2.20 2.03 1.58 -1.09 -3.33 1.48 -0.81 2.20 -1.29 -1.96 2.52 1.07 2.90 -1.60
Mitotic Spindle -2.62 -5.13 -6.17 -0.28 -3.86 -5.17 -4.51 -5.60 -1.69 -2.83 -5.74 -6.77 -8.34 -2.36 -4.76
None 1.10 -1.12 0.24 -2.23 4.30 5.17 5.09 6.52 2.21 5.33 5.07 4.28 6.01 1.06 6.69
Nuclear Periphery -0.16 -6.72 -1.36 -0.95 14.77 -0.18 -14.06 -2.78 -6.55 20.61 -0.02 -15.87 -3.75 -6.58 24.09
Nuclear Periphery Foci -2.94 -8.58 -6.21 -2.20 4.54 0.32 -14.38 -7.76 -6.22 10.56 -0.32 -16.79 -10.33 -7.21 11.07
Nucleolus -2.06 6.86 7.69 2.32 3.17 -2.53 5.02 6.23 4.74 5.87 -2.75 7.24 8.67 3.74 5.50
Nucleus 0.94 -29.52 -6.88 -6.94 26.49 16.66 -13.87 13.74 -1.96 25.89 14.77 -25.75 9.84 -6.01 36.81
Peroxisomes -0.68 9.46 8.21 3.40 -2.75 -6.88 0.23 -2.53 2.87 -2.20 -3.79 6.11 2.47 5.19 -3.13
Vacuole -6.13 -11.53 -29.83 -19.65 -18.28 -20.09 -29.00 -34.63 -22.87 -4.21 -19.36 -31.73 -48.55 -33.53 -15.43
Vacuole Periphery -1.33 8.69 0.79 1.54 -1.55 -2.59 4.99 2.31 2.94 -1.67 -2.74 9.18 0.32 2.62 -1.76
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pal2

Pal2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pal2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available