Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0.05 0.05 0.06 0 0.05 0.06
Bud 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.65 0.59 0.49 0.44 0.32 0.22 – 0.15 0.11 0.08 0.08 0.06 0.97 0.97 0.98 – – 0.83 0.12 0.13 0.06 0.11 0.12 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.08 0 0 0 0 0 0
Endosome 0.1 0.07 0.16 0.13 0.25 0 – 0 0 0 0 0 0 0 0 – – 0.06 0.11 0.11 0.1 0.07 0.07 0.08
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.17 0.25 0.26 0.36 0.33 0.79 – 0.8 0.92 0.89 0.96 0.92 0 0 0 – – 0 0.57 0.55 0.59 0.55 0.56 0.5
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.16 0.12 0 – 0.05 0 0.05 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.08 0.09 0.05 – 0.08 0.05 0.06 0 0.06 0 0 0 – – 0.06 0 0 0.05 0.05 0.05 0
Vac/Vac Membrane 0.08 0 0.06 0 0.07 0.05 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 0 0 0 – 0 0 0 0 0 0 0 2 – – 0 16 20 24 11 25 35
Bud 1 6 4 6 3 12 – 20 24 30 23 19 0 0 1 – – 0 1 5 3 3 8 16
Bud Neck 1 0 2 0 1 0 – 0 0 0 0 0 6 8 5 – – 3 3 4 4 7 16 15
Bud Site 0 0 0 0 2 2 – 8 5 10 6 7 0 0 0 – – 0 – – – – – –
Cell Periphery 2 1 2 6 1 1 – 1 0 3 0 2 3 3 1 – – 1 2 3 3 1 4 12
Cytoplasm 137 171 147 97 96 100 – 110 63 47 43 31 280 280 380 – – 171 37 53 23 28 65 74
Endoplasmic Reticulum 2 11 1 1 2 4 – 1 4 6 7 1 3 2 2 – – 17 4 5 10 3 5 11
Endosome 21 19 48 29 75 15 – 25 7 19 0 3 1 1 0 – – 13 33 44 40 19 36 45
Golgi 0 1 2 0 0 0 – 0 0 0 0 0 1 0 0 – – 0 2 6 6 3 3 3
Mitochondria 35 73 78 78 99 364 – 573 529 508 531 464 0 0 1 – – 8 177 225 242 143 308 290
Nucleus 1 0 2 0 2 0 – 8 6 9 4 4 0 0 1 – – 0 0 0 0 1 0 1
Nuclear Periphery 1 0 0 0 0 2 – 1 0 1 1 2 0 0 0 – – 1 0 0 1 0 0 3
Nucleolus 0 0 0 0 0 1 – 2 1 3 1 1 0 0 0 – – 0 0 0 1 0 3 1
Peroxisomes 8 2 31 34 37 6 – 39 1 26 2 19 0 0 0 – – 6 2 3 11 5 4 4
SpindlePole 7 7 29 17 26 23 – 59 27 34 8 32 0 0 2 – – 13 8 11 19 14 27 23
Vac/Vac Membrane 16 13 17 4 22 24 – 21 14 10 13 11 1 1 2 – – 5 13 14 4 14 27 32
Unique Cell Count 212 289 303 219 296 461 715 578 572 554 506 290 288 389 206 311 406 413 263 554 586
Labelled Cell Count 232 307 363 272 366 554 868 681 706 639 596 295 295 397 238 311 406 413 263 554 586


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 3.9 3.9 3.8 3.3 – 3.3 3.0 2.8 2.8 3.0 5.4 5.4 5.5 4.0 4.6 5.0 5.4 5.4 5.6
Std Deviation (1e-4) 0.7 0.6 1.4 1.6 1.0 1.3 – 1.0 0.8 0.7 0.8 0.7 1.5 1.5 1.2 1.0 1.1 1.4 1.7 1.5 1.6
Intensity Change (Log2) – – – -0.01 -0.06 -0.28 – -0.28 -0.39 -0.47 -0.47 -0.42 0.45 0.45 0.48 0.02 0.21 0.35 0.44 0.46 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1133 0.5711 0.7274 0.121 -0.1371 0.0815 2.1204 2.3795 2.5323 2.2194 1.7677 2.1566 1.9583 2.2535 1.8766 2.4431 2.3217 2.0584
Actin 0.0054 0.0141 0.0159 0.0338 0.0477 0.0037 0.0093 0.0034 0.0256 0.0004 0.0114 0.0073 0.0382 0.0109 0.027 0.0152 0.0227 0.0128
Bud 0.0003 0.0011 0.002 0.0014 0.0007 0.0012 0.0008 0.0019 0.0095 0.0009 0.0111 0.0013 0.0047 0.0088 0.0035 0.0012 0.0022 0.0004
Bud Neck 0.0004 0.0022 0.0037 0.0008 0.0008 0.0098 0.0009 0.001 0.0024 0.0002 0.0039 0.0028 0.0104 0.0013 0.0047 0.0155 0.0062 0.0081
Bud Periphery 0.0005 0.0013 0.0029 0.0037 0.0019 0.0022 0.002 0.0035 0.0181 0.0025 0.0234 0.0054 0.0164 0.0114 0.0091 0.0062 0.0063 0.0011
Bud Site 0.002 0.0311 0.0468 0.0031 0.0014 0.0036 0.0027 0.0145 0.0318 0.0002 0.003 0.0005 0.0111 0.0464 0.0219 0.0027 0.0059 0.0005
Cell Periphery 0.0014 0.0014 0.0014 0.0014 0.001 0.0016 0.0016 0.0015 0.0015 0.0005 0.0042 0.0019 0.0021 0.003 0.0014 0.0023 0.0014 0.0006
Cytoplasm 0.1854 0.2532 0.2213 0.0979 0.3861 0.2668 0.1033 0.1491 0.1561 0.1487 0.2414 0.2234 0.1023 0.2027 0.1879 0.1983 0.2137 0.2296
Cytoplasmic Foci 0.1156 0.1008 0.1138 0.0816 0.06 0.0928 0.1277 0.0849 0.0905 0.0198 0.0805 0.1025 0.158 0.0732 0.0964 0.0421 0.1146 0.0945
Eisosomes 0.0004 0.0009 0.0007 0.0009 0.0005 0.0007 0.0007 0.0007 0.0008 0.0004 0.0006 0.0006 0.0029 0.002 0.001 0.0009 0.0015 0.0004
Endoplasmic Reticulum 0.0074 0.013 0.0117 0.0059 0.0079 0.0075 0.0056 0.006 0.0042 0.0062 0.0029 0.0048 0.0038 0.0066 0.0084 0.0186 0.0027 0.0034
Endosome 0.1093 0.078 0.1604 0.1313 0.0722 0.0404 0.0648 0.047 0.0571 0.1066 0.0345 0.0408 0.0983 0.0559 0.0663 0.0773 0.0638 0.0647
Golgi 0.0139 0.0285 0.0402 0.0245 0.0217 0.0059 0.0101 0.0092 0.0182 0.0077 0.011 0.0125 0.0184 0.012 0.0106 0.0094 0.0263 0.0143
Lipid Particles 0.0369 0.0112 0.0253 0.0677 0.0036 0.0102 0.0455 0.0114 0.0106 0.001 0.0015 0.0097 0.0301 0.003 0.0124 0.0148 0.0141 0.0043
Mitochondria 0.0156 0.0132 0.0646 0.0676 0.0399 0.0102 0.0103 0.0273 0.0323 0.0663 0.0182 0.0519 0.0578 0.0348 0.0408 0.0446 0.0599 0.0479
None 0.4285 0.4312 0.2442 0.4425 0.3357 0.5265 0.5462 0.6121 0.5031 0.5983 0.5317 0.4902 0.3803 0.5036 0.4571 0.5104 0.4163 0.4897
Nuclear Periphery 0.007 0.0013 0.0032 0.0021 0.0011 0.0011 0.0141 0.0032 0.0035 0.002 0.0019 0.0012 0.0021 0.0013 0.0026 0.0059 0.001 0.0005
Nucleolus 0.001 0.0004 0.0005 0.0012 0.0003 0.0007 0.0015 0.0013 0.0019 0.0002 0.0012 0.0003 0.0048 0.0012 0.0019 0.001 0.0015 0.0003
Nucleus 0.0059 0.0009 0.0012 0.0014 0.0012 0.0012 0.0055 0.0029 0.005 0.001 0.002 0.0026 0.004 0.0022 0.0033 0.0025 0.0014 0.0007
Peroxisomes 0.0269 0.0099 0.0277 0.0133 0.0064 0.0076 0.0317 0.0054 0.0132 0.0007 0.0041 0.0321 0.032 0.0107 0.0248 0.0169 0.0203 0.0195
Punctate Nuclear 0.0193 0.0012 0.0017 0.01 0.0004 0.001 0.0045 0.007 0.0032 0.0003 0.006 0.0008 0.0122 0.0011 0.0076 0.0025 0.0013 0.001
Vacuole 0.0149 0.0038 0.0075 0.0051 0.0069 0.0041 0.008 0.0055 0.0091 0.0266 0.0042 0.0054 0.0081 0.0063 0.009 0.0084 0.0138 0.0042
Vacuole Periphery 0.002 0.0011 0.0032 0.0027 0.0024 0.001 0.0033 0.0013 0.0024 0.0097 0.0013 0.0018 0.0018 0.0015 0.0022 0.0033 0.0029 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2805 5.3362 7.3102 8.5188 7.1441 6.4249 11.6209 9.6641 9.7967 11.2052
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
263 769 572 1373 1719 1183 850 1413 1982 1952 1422 2786

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 885.39 687.98 858.27 859.70 697.73 755.27 853.10 838.02 722.63 728.76 855.18 848.70
Standard Deviation 172.76 103.14 109.82 131.52 90.98 168.06 95.65 125.20 123.25 149.63 101.62 128.81
Intensity Change Log 2 — -0.363946 -0.044882 -0.042480 — 0.114324 0.290046 0.264316 — -0.133449 0.112381 0.100828

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008485 0.002199 0.000345 0.003501 0.002176 0.000832 0.000328 0.003201 0.003013 0.001370 0.000335 0.003349
Bud Neck 0.025262 0.062352 0.003050 0.026874 0.055875 0.070121 0.005511 0.023369 0.051813 0.067060 0.004521 0.025096
Bud Site 0.067129 0.039325 0.004759 0.064453 0.028522 0.020497 0.010884 0.068452 0.033645 0.027915 0.008420 0.066482
Cell Periphery 0.002463 0.000203 0.000255 0.000302 0.000248 0.000235 0.000143 0.000244 0.000542 0.000223 0.000188 0.000272
Cytoplasm 0.251086* 0.170519* 0.321966* 0.360427* 0.202987* 0.109482* 0.268955* 0.390263* 0.209369* 0.133528* 0.290279* 0.375559*
Cytoplasmic Foci 0.081422 0.277242* 0.037057 0.037973 0.343738* 0.272525* 0.013245 0.072917 0.308930* 0.274384* 0.022823 0.055696
Eisosomes 0.000242 0.000246 0.000102 0.000086 0.000539 0.000281 0.000049 0.000103 0.000500 0.000267 0.000070 0.000095
Endoplasmic Reticulum 0.005538 0.001530 0.003935 0.003913 0.001708 0.000953 0.004476 0.002989 0.002216 0.001180 0.004259 0.003445
Endosome 0.021463 0.068032 0.004858 0.009802 0.045443 0.104252 0.002745 0.011789 0.042261 0.089983 0.003595 0.010810
Golgi 0.005807 0.024453 0.000159 0.005741 0.022505 0.031976 0.000077 0.005576 0.020289 0.029012 0.000110 0.005657
Lipid Particles 0.003349 0.014669 0.004878 0.002511 0.022015 0.025832 0.001800 0.002619 0.019538 0.021434 0.003038 0.002566
Mitochondria 0.007283 0.065132 0.000497 0.011416 0.070983 0.126109* 0.000989 0.009619 0.062530 0.102087* 0.000791 0.010505
Mitotic Spindle 0.014422 0.024975 0.005663 0.082858 0.007017 0.012345 0.002330 0.051047 0.008000 0.017320 0.003671 0.066724
None 0.002006 0.003034 0.009742 0.008563 0.001154 0.001946 0.005729 0.003358 0.001267 0.002374 0.007343 0.005923
Nuclear Periphery 0.002658 0.000624 0.001880 0.003492 0.000464 0.000809 0.002759 0.001386 0.000756 0.000736 0.002405 0.002423
Nuclear Periphery Foci 0.002076 0.000757 0.001869 0.002569 0.000816 0.000555 0.001189 0.001054 0.000983 0.000634 0.001463 0.001801
Nucleolus 0.001029 0.002949 0.001780 0.001350 0.001269 0.003872 0.000925 0.001029 0.001237 0.003509 0.001269 0.001187
Nucleus 0.158213* 0.061868 0.522612* 0.205448* 0.061372 0.048402 0.619203* 0.192419* 0.074222 0.053707 0.580350* 0.198840*
Peroxisomes 0.020455 0.090825 0.001064 0.009719 0.062377 0.066241 0.000329 0.019585 0.056814 0.075926 0.000624 0.014723
Vacuole 0.303367* 0.083850 0.073209 0.150003 0.064723 0.094213 0.057699 0.135596 0.096390 0.090130 0.063938 0.142696
Vacuole Periphery 0.016245 0.005217 0.000319 0.008998 0.004067 0.008521 0.000636 0.003386 0.005683 0.007220 0.000509 0.006152

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.73 2.69 1.83 0.50 -4.18 3.23 4.29 0.13 -3.92 -5.40 2.74 5.03 1.12 -2.49 -6.70
Bud Neck -7.91 6.06 0.67 9.52 -10.85 -3.08 18.89 13.01 12.65 -9.21 -4.20 20.10 13.31 15.85 -13.68
Bud Site 1.29 5.57 1.99 1.38 -8.86 2.96 6.63 -3.43 -5.68 -8.34 1.29 9.47 -2.18 -3.66 -11.66
Cell Periphery 1.29 1.25 1.31 0.77 2.51 0.53 5.49 3.42 2.62 -2.10 1.41 1.70 1.72 2.49 0.24
Cytoplasm 3.87 -2.33 -2.39 -10.05 0.24 12.55 -1.53 -14.30 -25.53 -11.04 10.69 -4.42 -13.67 -25.66 -7.84
Cytoplasmic Foci -19.22 5.04 6.46 31.14 2.73 8.31 59.91 47.54 31.42 -15.20 4.69 53.29 48.83 44.79 -9.76
Eisosomes -0.99 2.02 2.56 7.93 2.76 4.40 8.47 7.84 10.46 -6.60 4.47 8.41 8.22 13.25 -2.14
Endoplasmic Reticulum 5.41 0.40 1.53 -11.73 1.83 2.37 -11.28 -5.22 -10.07 7.42 3.66 -10.63 -6.17 -15.95 6.71
Endosome -9.88 3.72 3.86 13.97 0.43 -12.16 19.94 16.88 22.07 -5.95 -12.94 19.72 18.13 26.02 -4.66
Golgi -6.92 1.84 0.85 9.10 -2.58 -4.21 18.77 14.13 14.51 -3.66 -4.76 18.52 15.03 17.16 -4.47
Lipid Particles -7.54 -1.51 1.77 8.26 3.08 -1.81 16.99 16.36 12.52 -4.21 -1.26 15.70 16.28 14.84 1.01
Mitochondria -10.87 1.99 -0.17 11.43 -4.72 -7.79 20.70 18.44 19.29 -4.19 -7.43 20.54 18.55 22.16 -6.17
Mitotic Spindle -1.14 1.96 -5.84 -5.46 -9.78 -2.35 1.27 -8.77 -6.67 -9.07 -4.00 1.63 -12.96 -8.94 -13.52
None -1.32 -3.80 -3.70 -1.82 1.23 -1.95 -6.38 -5.73 -2.75 1.73 -2.51 -6.71 -6.67 -3.14 1.61
Nuclear Periphery 2.39 0.21 -2.41 -12.61 -6.87 -1.90 -20.28 -16.27 -8.51 5.16 -0.14 -16.15 -15.18 -14.52 -1.85
Nuclear Periphery Foci 2.79 -0.27 0.37 -3.09 0.83 0.83 -1.77 -1.09 -4.01 1.34 1.04 -2.70 -2.14 -4.96 0.96
Nucleolus -3.80 -1.80 -0.80 3.63 1.40 -4.78 3.10 -0.09 4.63 -2.68 -5.80 1.32 0.15 5.97 -1.30
Nucleus 6.95 -18.69 -6.13 -23.65 18.09 2.97 -56.26 -25.87 -26.93 32.38 4.79 -60.72 -31.69 -36.50 36.09
Peroxisomes -11.07 3.19 2.41 15.28 -3.48 -0.81 23.03 16.20 14.70 -7.45 -4.96 22.77 18.54 21.61 -8.32
Vacuole 14.40 12.49 4.14 -20.69 -15.29 -5.76 -6.17 -22.27 -15.83 -13.17 2.15 -0.77 -22.53 -25.76 -20.47
Vacuole Periphery 1.03 2.20 1.56 2.36 -3.31 -6.37 10.36 4.85 9.05 -2.89 -3.17 7.22 3.43 7.92 -4.27
Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
Localization
Cell Percentages mitochondrion (40%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pet111

Pet111


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet111-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available