| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Plasma membrane (PM) P2-type H+-ATPase; pumps protons out of the cell; major regulator of cytoplasmic pH and PM potential; long-lived hexameric protein asymmetrically distributed between mother cells and buds; buds emerge with very low levels of Pma1p, while newborn cells contain low levels, and mothers accumulate high levels during aging; has several calcium-binding sites in the nucleotide binding domain; regulated by Hsp30p; atypical yeast prion [GAR+] is derived from the Pma1p-Std1p complex |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 3 | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | – |
| Vac/Vac Membrane | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 3 | – | – | – | – |
| Unique Cell Count | 1 | 17 | ||||||||||||||||||||||
| Labelled Cell Count | 1 | 17 | ||||||||||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Std Deviation (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 1029.9642 | 2080.0838 | 4353.3727 | 3392.8598 | 1893.213 | 1199.593 | 2570.6323 | 3240.2602 | 3870.8942 | 1980.2259 |
| Translational Efficiency | 1.0124 | 1.0524 | 1.007 | 1.2528 | 0.9673 | 0.8571 | 1.0163 | 0.9506 | 0.8955 | 0.901 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 112 | 11 | 109 | N/A | 19 | 5 | N/A | N/A | 131 | 16 | 109 | 0 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 6058.75 | 276.67 | 11179.41 | N/A | 288.20 | 288.72 | N/A | N/A | 5221.80 | 280.44 | 11179.41 | — |
| Standard Deviation | 1767.71 | 3.71 | 3831.04 | N/A | 5.25 | 5.13 | N/A | N/A | 2607.83 | 6.99 | 3831.04 | — |
| Intensity Change Log 2 | — | -4.452782 | 0.883752 | N/A | — | 0.002601 | N/A | N/A | — | -3.488745 | — | — |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.004983 | 0.000014 | 0.002443 | N/A | 0.000103 | 0.000017 | N/A | N/A | 0.004275 | 0.000015 | 0.002443 | N/A |
| Bud Neck | 0.010209 | 0.000004 | 0.009378 | N/A | 0.000057 | 0.000007 | N/A | N/A | 0.008736 | 0.000005 | 0.009378 | N/A |
| Bud Site | 0.020531 | 0.000008 | 0.016939 | N/A | 0.000091 | 0.000012 | N/A | N/A | 0.017567 | 0.000009 | 0.016939 | N/A |
| Cell Periphery | 0.485654* | 0.000035 | 0.246455* | N/A | 0.000186 | 0.000042 | N/A | N/A | 0.415243* | 0.000037 | 0.246455* | N/A |
| Cytoplasm | 0.001678 | 0.000019 | 0.002645 | N/A | 0.000094 | 0.000023 | N/A | N/A | 0.001449 | 0.000020 | 0.002645 | N/A |
| Cytoplasmic Foci | 0.004390 | 0.000008 | 0.004443 | N/A | 0.000032 | 0.000010 | N/A | N/A | 0.003758 | 0.000009 | 0.004443 | N/A |
| Eisosomes | 0.019743 | 0.000042 | 0.002509 | N/A | 0.000366 | 0.000020 | N/A | N/A | 0.016932 | 0.000035 | 0.002509 | N/A |
| Endoplasmic Reticulum | 0.019966 | 0.000000 | 0.113538* | N/A | 0.000002 | 0.000001 | N/A | N/A | 0.017071 | 0.000001 | 0.113538* | N/A |
| Endosome | 0.007865 | 0.000001 | 0.016144 | N/A | 0.000003 | 0.000001 | N/A | N/A | 0.006725 | 0.000001 | 0.016144 | N/A |
| Golgi | 0.011210 | 0.000003 | 0.020947 | N/A | 0.000012 | 0.000004 | N/A | N/A | 0.009586 | 0.000004 | 0.020947 | N/A |
| Lipid Particles | 0.074268 | 0.000014 | 0.149767* | N/A | 0.000066 | 0.000029 | N/A | N/A | 0.063506 | 0.000018 | 0.149767* | N/A |
| Mitochondria | 0.000834 | 0.000007 | 0.001086 | N/A | 0.000024 | 0.000010 | N/A | N/A | 0.000716 | 0.000008 | 0.001086 | N/A |
| Mitotic Spindle | 0.001418 | 0.000202 | 0.000426 | N/A | 0.000369 | 0.000295 | N/A | N/A | 0.001266 | 0.000231 | 0.000426 | N/A |
| None | 0.000069 | 0.999497* | 0.000248 | N/A | 0.998250* | 0.999404* | N/A | N/A | 0.144843* | 0.999468* | 0.000248 | N/A |
| Nuclear Periphery | 0.007691 | 0.000000 | 0.005513 | N/A | 0.000001 | 0.000000 | N/A | N/A | 0.006576 | 0.000000 | 0.005513 | N/A |
| Nuclear Periphery Foci | 0.000642 | 0.000065 | 0.004065 | N/A | 0.000041 | 0.000038 | N/A | N/A | 0.000555 | 0.000056 | 0.004065 | N/A |
| Nucleolus | 0.000084 | 0.000025 | 0.000154 | N/A | 0.000052 | 0.000038 | N/A | N/A | 0.000079 | 0.000029 | 0.000154 | N/A |
| Nucleus | 0.000189 | 0.000007 | 0.001338 | N/A | 0.000025 | 0.000012 | N/A | N/A | 0.000166 | 0.000009 | 0.001338 | N/A |
| Peroxisomes | 0.000457 | 0.000046 | 0.000522 | N/A | 0.000225 | 0.000036 | N/A | N/A | 0.000423 | 0.000043 | 0.000522 | N/A |
| Vacuole | 0.321980* | 0.000000 | 0.382750* | N/A | 0.000003 | 0.000001 | N/A | N/A | 0.275281* | 0.000001 | 0.382750* | N/A |
| Vacuole Periphery | 0.006138 | 0.000000 | 0.018690 | N/A | 0.000000 | 0.000000 | N/A | N/A | 0.005248 | 0.000000 | 0.018690 | N/A |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Neck | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Site | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cell Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasm | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasmic Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Eisosomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endoplasmic Reticulum | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endosome | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Golgi | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Lipid Particles | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitochondria | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitotic Spindle | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| None | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleolus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Peroxisomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Plasma membrane (PM) P2-type H+-ATPase; pumps protons out of the cell; major regulator of cytoplasmic pH and PM potential; long-lived hexameric protein asymmetrically distributed between mother cells and buds; buds emerge with very low levels of Pma1p, while newborn cells contain low levels, and mothers accumulate high levels during aging; has several calcium-binding sites in the nucleotide binding domain; regulated by Hsp30p; atypical yeast prion [GAR+] is derived from the Pma1p-Std1p complex |
| Localization | |
| Cell Percentages | — |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Pma1
Pma1
Nearest neighbours ( genes)
correlation threshold
0.80
