Standard name | |
---|---|
Human Ortholog | |
Description | Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; homologous to human NUP210 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 3 | 2 | 2 | 3 | 5 |
Bud | 1 | 0 | 0 | 3 | 1 | 10 | 8 | 13 | 15 | 17 | 14 | 9 | 0 | 0 | 0 | 1 | 3 | 0 | 6 | 2 | 1 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 0 | 1 | 0 | 6 | 10 | 9 | 14 | 10 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 21 | 24 | 25 | 20 | 26 | 29 | 46 | 22 | 17 | 22 | 7 | 30 | 30 | 29 | 62 | 51 | 25 | 4 | 6 | 4 | 3 | 3 | 2 |
Endoplasmic Reticulum | 1 | 6 | 1 | 2 | 0 | 4 | 2 | 0 | 2 | 5 | 2 | 3 | 16 | 10 | 10 | 14 | 8 | 6 | 6 | 5 | 4 | 5 | 3 | 6 |
Endosome | 93 | 15 | 139 | 34 | 70 | 19 | 13 | 20 | 3 | 5 | 2 | 1 | 12 | 10 | 11 | 63 | 61 | 47 | 50 | 44 | 59 | 48 | 75 | 101 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 1 | 7 | 3 | 13 | 10 |
Mitochondria | 140 | 104 | 159 | 156 | 164 | 453 | 565 | 517 | 520 | 516 | 479 | 432 | 3 | 3 | 1 | 24 | 22 | 19 | 42 | 30 | 33 | 52 | 61 | 62 |
Nucleus | 24 | 4 | 4 | 10 | 16 | 25 | 32 | 53 | 42 | 38 | 43 | 46 | 6 | 6 | 8 | 3 | 6 | 1 | 5 | 6 | 0 | 2 | 1 | 1 |
Nuclear Periphery | 68 | 54 | 56 | 73 | 59 | 73 | 149 | 84 | 107 | 108 | 113 | 87 | 33 | 32 | 21 | 11 | 13 | 5 | 378 | 153 | 268 | 97 | 119 | 101 |
Nucleolus | 13 | 1 | 8 | 8 | 9 | 22 | 31 | 35 | 27 | 43 | 50 | 27 | 0 | 0 | 0 | 3 | 2 | 1 | 6 | 5 | 2 | 2 | 2 | 1 |
Peroxisomes | 1 | 0 | 7 | 2 | 5 | 2 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 | 12 | 16 | 17 |
SpindlePole | 5 | 3 | 14 | 7 | 8 | 8 | 18 | 12 | 9 | 8 | 9 | 4 | 1 | 0 | 3 | 22 | 17 | 11 | 28 | 12 | 15 | 11 | 10 | 9 |
Vac/Vac Membrane | 287 | 152 | 104 | 49 | 99 | 68 | 56 | 53 | 68 | 48 | 60 | 44 | 62 | 69 | 70 | 50 | 69 | 48 | 28 | 23 | 5 | 10 | 10 | 11 |
Unique Cell Count | 472 | 256 | 352 | 269 | 326 | 543 | 650 | 639 | 586 | 569 | 549 | 472 | 133 | 137 | 132 | 198 | 192 | 130 | 580 | 303 | 419 | 264 | 331 | 342 |
Labelled Cell Count | 654 | 361 | 516 | 371 | 451 | 717 | 917 | 844 | 830 | 815 | 801 | 670 | 163 | 160 | 154 | 254 | 256 | 168 | 580 | 303 | 419 | 264 | 331 | 342 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.1 | 4.7 | 4.7 | 4.6 | 3.9 | 3.5 | 3.9 | 3.6 | 3.4 | 3.5 | 3.6 | 6.0 | 6.0 | 6.1 | 5.1 | 5.5 | 6.0 | 6.0 | 6.6 | 7.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.3 | 1.6 | 2.3 | 1.4 | 1.0 | 1.3 | 1.1 | 0.9 | 0.8 | 0.7 | 0.9 | 0.9 | 0.9 | 1.7 | 1.7 | 2.1 | 1.3 | 1.8 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.01 | -0.28 | -0.42 | -0.27 | -0.38 | -0.45 | -0.43 | -0.36 | 0.35 | 0.35 | 0.37 | 0.12 | 0.24 | 0.37 | 0.36 | 0.51 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | -0.4 | -1.3 | -1.6 | 0.2 | -2.1 | -2.7 | -1.9 | -4.0 | 4.9 | 4.8 | 4.8 | 7.6 | 6.4 | 4.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.5 | -6.9 | -6.6 | -1.8 | -1.8 | -0.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -8.9 | -9.1 | -9.3 | -7.9 | -8.0 | -6.2 |
Nucleus | 2.1 | 2.9 | 2.9 | 3.1 | 4.6 | 4.1 | 3.9 | 4.4 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 1.9 | 0 | -3.6 | -3.1 | -3.5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 2.3 | 2.0 |
Vacuole | -3.2 | 0.2 | -6.3 | -8.6 | -8.8 | -6.9 | -8.4 | -7.1 | -7.5 | 3.5 | 4.3 | 4.8 | -1.1 | 1.5 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0317 | 0.0005 | 0.0133 | 0.0015 | 0.0152 | 0.0019 | 0.0059 | 0.0071 | 0.0048 | 0.0107 | 0.0061 | 0.0003 | 0.0125 | 0.003 | 0.0036 | 0.0054 | 0.0115 | 0.0021 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0012 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.005 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.0008 | 0.0001 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0 | 0.0022 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0003 |
Bud Site | 0.0023 | 0.0012 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.0027 | 0.0001 | 0.0006 | 0.0004 | 0 | 0.003 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0006 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0033 | 0.0039 | 0.0006 | 0.0021 | 0.0007 | 0.0006 | 0.0027 | 0.0006 | 0.0014 | 0.0074 | 0.0005 | 0.0005 | 0.0047 | 0.0023 | 0.0063 | 0.0008 | 0.0002 | 0.0008 |
Cytoplasmic Foci | 0.0172 | 0.0011 | 0.0015 | 0.0004 | 0.0068 | 0.0011 | 0.0196 | 0.0009 | 0.0026 | 0.0007 | 0.0021 | 0.0001 | 0.0063 | 0.0002 | 0.0007 | 0.0353 | 0.0007 | 0.0003 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0068 | 0.0043 | 0.0034 | 0.0038 | 0.0035 | 0.0018 | 0.0068 | 0.0168 | 0.0068 | 0.005 | 0.0076 | 0.0026 | 0.0136 | 0.0177 | 0.0076 | 0.0081 | 0.0073 | 0.004 |
Endosome | 0.0294 | 0.0068 | 0.0061 | 0.0036 | 0.0216 | 0.006 | 0.0255 | 0.0035 | 0.0113 | 0.0037 | 0.0153 | 0.0015 | 0.0917 | 0.005 | 0.0043 | 0.018 | 0.0108 | 0.0047 |
Golgi | 0.0088 | 0.0005 | 0.0017 | 0.0012 | 0.0127 | 0.0012 | 0.007 | 0.0013 | 0.0037 | 0.0016 | 0.0055 | 0.0002 | 0.0112 | 0.0006 | 0.0004 | 0.0296 | 0.0076 | 0.0008 |
Lipid Particles | 0.0409 | 0.0055 | 0.0101 | 0.0265 | 0.0308 | 0.0051 | 0.0415 | 0.0087 | 0.0177 | 0.0039 | 0.0346 | 0.0018 | 0.0607 | 0.0022 | 0.0011 | 0.0144 | 0.0261 | 0.0036 |
Mitochondria | 0.0132 | 0.0017 | 0.0026 | 0.0033 | 0.0058 | 0.0025 | 0.0043 | 0.0206 | 0.004 | 0.0455 | 0.0031 | 0.0008 | 0.0112 | 0.0035 | 0.0026 | 0.0033 | 0.0133 | 0.0026 |
None | 0.0043 | 0.0022 | 0.0008 | 0.0014 | 0.0016 | 0.0006 | 0.0042 | 0.0009 | 0.0151 | 0.0034 | 0.0011 | 0.0047 | 0.0037 | 0.0014 | 0.0047 | 0.0008 | 0.0013 | 0.0008 |
Nuclear Periphery | 0.7713 | 0.8768 | 0.879 | 0.8603 | 0.8121 | 0.9196 | 0.7878 | 0.8797 | 0.8749 | 0.8456 | 0.8721 | 0.9378 | 0.5135 | 0.8198 | 0.8539 | 0.8276 | 0.8693 | 0.898 |
Nucleolus | 0.0034 | 0.0029 | 0.0051 | 0.0104 | 0.0121 | 0.0027 | 0.0026 | 0.0007 | 0.0006 | 0.0008 | 0.0034 | 0.0015 | 0.0421 | 0.0007 | 0.0012 | 0.0002 | 0.0019 | 0.0049 |
Nucleus | 0.015 | 0.0561 | 0.0275 | 0.0452 | 0.0315 | 0.0328 | 0.0164 | 0.022 | 0.0142 | 0.0306 | 0.014 | 0.0266 | 0.1028 | 0.1095 | 0.0732 | 0.0113 | 0.0104 | 0.051 |
Peroxisomes | 0.0138 | 0.0002 | 0.0006 | 0.0003 | 0.0054 | 0.0004 | 0.0246 | 0.0018 | 0.0014 | 0.0014 | 0.0014 | 0.0001 | 0.0034 | 0.0001 | 0.0002 | 0.0238 | 0.0006 | 0.0002 |
Punctate Nuclear | 0.0085 | 0.006 | 0.0096 | 0.0123 | 0.0175 | 0.0083 | 0.0255 | 0.002 | 0.0042 | 0.0106 | 0.0123 | 0.0048 | 0.068 | 0.0022 | 0.0062 | 0.0031 | 0.0037 | 0.0088 |
Vacuole | 0.006 | 0.0027 | 0.0058 | 0.0011 | 0.0019 | 0.0008 | 0.0015 | 0.0014 | 0.0016 | 0.0009 | 0.0025 | 0.0023 | 0.0227 | 0.0018 | 0.0012 | 0.0006 | 0.002 | 0.0013 |
Vacuole Periphery | 0.0212 | 0.027 | 0.0304 | 0.0259 | 0.0189 | 0.0139 | 0.0217 | 0.0279 | 0.0349 | 0.0258 | 0.017 | 0.0143 | 0.0198 | 0.0294 | 0.0314 | 0.0171 | 0.0319 | 0.0146 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.2616 | 30.471 | 30.634 | 34.6366 | 30.2945 | 18.4074 | 36.5238 | 41.5883 | 30.6373 | 41.052 |
Translational Efficiency | 0.6112 | 0.6741 | 0.6327 | 0.429 | 0.5033 | 0.9875 | 0.6321 | 0.4236 | 0.5907 | 0.4896 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
456 | 1390 | 326 | 765 | 2036 | 1790 | 611 | 1933 | 2492 | 3180 | 937 | 2698 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 796.68 | 743.95 | 932.19 | 903.19 | 696.08 | 783.11 | 913.83 | 860.07 | 714.49 | 765.99 | 920.22 | 872.30 |
Standard Deviation | 109.92 | 105.73 | 103.91 | 135.42 | 90.03 | 104.01 | 107.76 | 112.89 | 101.71 | 106.55 | 106.80 | 121.28 |
Intensity Change Log 2 | — | -0.098795 | 0.226624 | 0.181029 | — | 0.169962 | 0.392673 | 0.305201 | — | 0.032775 | 0.306436 | 0.240263 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000255 | 0.000171 | 0.000890 | 0.001771 | 0.000113 | 0.000168 | 0.000215 | 0.000314 | 0.000139 | 0.000169 | 0.000450 | 0.000727 |
Bud Neck | 0.001368 | 0.000748 | 0.001789 | 0.010142 | 0.000232 | 0.000753 | 0.000807 | 0.004946 | 0.000440 | 0.000751 | 0.001148 | 0.006419 |
Bud Site | 0.000881 | 0.000295 | 0.000488 | 0.039618 | 0.000165 | 0.000424 | 0.000146 | 0.007912 | 0.000296 | 0.000368 | 0.000265 | 0.016902 |
Cell Periphery | 0.000060 | 0.000056 | 0.000064 | 0.000126 | 0.000063 | 0.000073 | 0.000019 | 0.000076 | 0.000062 | 0.000066 | 0.000035 | 0.000090 |
Cytoplasm | 0.019647 | 0.022058 | 0.007758 | 0.041761 | 0.003172 | 0.033305 | 0.005980 | 0.034580 | 0.006187 | 0.028389 | 0.006599 | 0.036616 |
Cytoplasmic Foci | 0.018567 | 0.009772 | 0.001874 | 0.040758 | 0.004308 | 0.015394 | 0.000275 | 0.017098 | 0.006918 | 0.012936 | 0.000831 | 0.023807 |
Eisosomes | 0.000043 | 0.000021 | 0.000052 | 0.000040 | 0.000011 | 0.000025 | 0.000009 | 0.000025 | 0.000017 | 0.000023 | 0.000024 | 0.000030 |
Endoplasmic Reticulum | 0.015954 | 0.012199 | 0.023703 | 0.007165 | 0.007966 | 0.017165 | 0.008911 | 0.009034 | 0.009427 | 0.014994 | 0.014058 | 0.008504 |
Endosome | 0.022254 | 0.009094 | 0.003409 | 0.063961 | 0.006525 | 0.011113 | 0.001346 | 0.033322 | 0.009403 | 0.010230 | 0.002064 | 0.042010 |
Golgi | 0.001963 | 0.002217 | 0.000134 | 0.011421 | 0.002088 | 0.002650 | 0.000064 | 0.001870 | 0.002065 | 0.002461 | 0.000088 | 0.004578 |
Lipid Particles | 0.056601 | 0.071215 | 0.006531 | 0.019289 | 0.124716* | 0.133552* | 0.001652 | 0.034859 | 0.112252* | 0.106304* | 0.003350 | 0.030444 |
Mitochondria | 0.004966 | 0.009813 | 0.002951 | 0.006068 | 0.009224 | 0.017337 | 0.001295 | 0.004739 | 0.008445 | 0.014048 | 0.001871 | 0.005116 |
Mitotic Spindle | 0.065730 | 0.035371 | 0.010345 | 0.511617* | 0.007639 | 0.019953 | 0.020924 | 0.278705* | 0.018268 | 0.026693 | 0.017244 | 0.344746* |
None | 0.001953 | 0.007729 | 0.001701 | 0.001140 | 0.004686 | 0.010648 | 0.001433 | 0.002486 | 0.004186 | 0.009372 | 0.001526 | 0.002105 |
Nuclear Periphery | 0.205771* | 0.136902* | 0.299139* | 0.027386 | 0.095795 | 0.226925* | 0.360782* | 0.134104* | 0.115919* | 0.187575* | 0.339335* | 0.103845* |
Nuclear Periphery Foci | 0.475726* | 0.614107* | 0.322723* | 0.111742 | 0.710051* | 0.436941* | 0.506280* | 0.361316* | 0.667173* | 0.514382* | 0.442417* | 0.290551* |
Nucleolus | 0.029674 | 0.023609 | 0.056835 | 0.004341 | 0.010640 | 0.017111 | 0.023953 | 0.014460 | 0.014123 | 0.019951 | 0.035393 | 0.011591 |
Nucleus | 0.056274 | 0.038322 | 0.242482* | 0.010724 | 0.009233 | 0.044734 | 0.062394 | 0.030149 | 0.017841 | 0.041931 | 0.125050 | 0.024641 |
Peroxisomes | 0.003814 | 0.001298 | 0.000959 | 0.009438 | 0.000976 | 0.002039 | 0.000163 | 0.002078 | 0.001495 | 0.001716 | 0.000440 | 0.004165 |
Vacuole | 0.016531 | 0.003080 | 0.015175 | 0.076028 | 0.001091 | 0.005998 | 0.002968 | 0.024037 | 0.003916 | 0.004723 | 0.007215 | 0.038779 |
Vacuole Periphery | 0.001968 | 0.001921 | 0.000999 | 0.005465 | 0.001307 | 0.003691 | 0.000386 | 0.003891 | 0.001428 | 0.002917 | 0.000599 | 0.004337 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.37 | -6.84 | -4.88 | -5.32 | -1.97 | -1.41 | -4.07 | -3.78 | -2.85 | -1.59 | -0.86 | -8.36 | -6.29 | -6.00 | -1.93 |
Bud Neck | 3.76 | -2.12 | -9.03 | -10.17 | -8.13 | -5.77 | -3.86 | -13.16 | -11.75 | -10.43 | -3.01 | -5.88 | -16.03 | -15.43 | -12.82 |
Bud Site | 3.51 | 1.09 | -9.14 | -9.38 | -9.22 | -2.61 | -0.49 | -7.69 | -7.42 | -7.65 | -0.24 | -0.99 | -11.75 | -11.74 | -11.69 |
Cell Periphery | 1.17 | -1.77 | -3.21 | -3.89 | -1.83 | -1.48 | 7.75 | -1.93 | -0.54 | -7.44 | -0.61 | 3.63 | -3.48 | -3.02 | -6.01 |
Cytoplasm | 0.60 | 4.02 | -4.94 | -6.13 | -9.21 | -12.51 | -2.49 | -15.35 | -3.20 | -12.69 | -11.98 | -0.71 | -16.54 | -6.56 | -15.66 |
Cytoplasmic Foci | 3.17 | 5.33 | -3.54 | -9.31 | -12.20 | -8.25 | 5.70 | -10.78 | -2.31 | -16.34 | -4.93 | 6.97 | -11.71 | -7.96 | -19.79 |
Eisosomes | 6.14 | -2.11 | 0.42 | -8.63 | 2.62 | -9.02 | -0.18 | -11.23 | -3.41 | -9.07 | -3.34 | -4.18 | -9.85 | -8.00 | -1.59 |
Endoplasmic Reticulum | 1.58 | -3.78 | 3.58 | 3.91 | 9.89 | -7.98 | -1.76 | -1.55 | 7.05 | 0.52 | -6.17 | -5.87 | 0.90 | 7.77 | 7.17 |
Endosome | 4.81 | 6.47 | -6.68 | -11.87 | -13.27 | -4.19 | 7.87 | -14.70 | -11.14 | -18.14 | -0.76 | 9.05 | -16.72 | -16.35 | -22.12 |
Golgi | -0.57 | 2.92 | -3.11 | -2.94 | -4.31 | -0.92 | 6.67 | 0.96 | 1.67 | -3.66 | -0.88 | 7.22 | -1.79 | -1.21 | -5.59 |
Lipid Particles | -2.25 | 8.08 | 6.06 | 12.49 | -5.22 | -1.36 | 25.51 | 17.58 | 19.43 | -16.72 | 1.03 | 26.21 | 18.94 | 21.83 | -16.92 |
Mitochondria | -3.34 | 1.30 | 0.39 | 5.20 | -1.35 | -5.60 | 8.99 | 4.47 | 9.94 | -6.85 | -5.46 | 8.53 | 4.40 | 11.27 | -6.15 |
Mitotic Spindle | 3.89 | 5.35 | -22.97 | -29.23* | -29.72* | -5.59 | -4.02 | -34.57* | -32.23* | -29.52* | -2.82 | -0.69 | -42.61 | -41.73* | -40.43* |
None | -5.79 | 0.84 | 2.99 | 7.29 | 2.45 | -4.14 | 3.71 | 2.21 | 5.80 | -2.45 | -5.24 | 3.86 | 2.90 | 8.14 | -1.75 |
Nuclear Periphery | 4.83 | -3.76 | 14.95 | 19.98* | 15.83 | -19.44* | -22.11 | -5.25 | 13.83 | 19.06 | -13.92 | -21.60 | 3.95 | 17.32 | 23.45 |
Nuclear Periphery Foci | -8.97 | 6.78 | 20.18* | 43.62* | 11.25 | 27.13* | 15.36 | 39.57* | 9.99 | 12.47 | 17.51 | 19.54 | 46.22 | 28.81* | 14.41 |
Nucleolus | 1.44 | -4.17 | 6.96 | 9.44 | 10.58 | -3.75 | -4.59 | -4.64 | -1.11 | 1.61 | -3.84 | -7.60 | -0.76 | 3.21 | 7.26 |
Nucleus | 3.44 | -11.52 | 6.91 | 6.39 | 15.37 | -11.89 | -8.99 | -11.53 | 1.52 | 4.15 | -8.99 | -15.06 | -5.86 | 3.47 | 13.18 |
Peroxisomes | 1.49 | 1.65 | -1.92 | -6.17 | -6.53 | -2.24 | 4.04 | -3.25 | -0.73 | -7.45 | -0.19 | 3.06 | -4.34 | -5.23 | -9.34 |
Vacuole | 5.71 | -0.34 | -12.61 | -16.69 | -11.96 | -7.12 | -3.05 | -14.73 | -11.75 | -11.85 | 0.67 | -3.59 | -19.08 | -19.65 | -14.82 |
Vacuole Periphery | -0.16 | 1.65 | -2.20 | -2.39 | -3.75 | -4.73 | 4.06 | -3.01 | 0.41 | -4.50 | -4.47 | 3.91 | -3.80 | -0.94 | -5.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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