Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate; human ortholog, PPA2, functionally complements the null mutant; mutations in human PPA2 cause a mitochondrial disease resulting in sudden unexpected cardiac arrest in infants |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 8 | 3 | 3 | 2 | 0 | 2 | 6 | 11 | 15 |
Bud | 7 | – | 0 | 3 | 3 | 2 | 22 | 15 | 11 | 10 | 17 | 10 | 2 | 1 | 0 | 8 | 5 | 10 | 0 | 0 | 0 | 2 | 6 | 12 |
Bud Neck | 0 | – | 2 | 9 | 20 | 9 | 15 | 25 | 9 | 12 | 4 | 9 | 7 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 6 | 12 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 4 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 6 | 5 | 1 | 3 | 3 | 2 | 1 | 1 | 2 | 3 | 8 | 4 | 9 | 21 | 13 | 0 | 0 | 0 | 0 | 2 | 5 |
Cytoplasm | 57 | – | 25 | 240 | 447 | 278 | 502 | 338 | 169 | 241 | 171 | 243 | 257 | 366 | 446 | 25 | 34 | 28 | 35 | 22 | 16 | 77 | 101 | 156 |
Endoplasmic Reticulum | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 15 | 13 | 8 | 2 | 0 | 0 | 0 | 1 | 6 |
Endosome | 0 | – | 1 | 3 | 9 | 7 | 5 | 4 | 2 | 1 | 0 | 3 | 5 | 18 | 11 | 26 | 34 | 38 | 5 | 0 | 4 | 7 | 14 | 14 |
Golgi | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 30 | 20 | 22 | 0 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | 77 | – | 2 | 14 | 32 | 87 | 243 | 160 | 148 | 162 | 194 | 170 | 10 | 9 | 3 | 28 | 31 | 26 | 62 | 14 | 26 | 69 | 99 | 116 |
Nucleus | 0 | – | 0 | 0 | 1 | 3 | 10 | 9 | 9 | 6 | 4 | 3 | 0 | 5 | 3 | 4 | 3 | 2 | 0 | 1 | 0 | 0 | 3 | 3 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | – | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 1 | 4 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Peroxisomes | 0 | – | 0 | 1 | 0 | 2 | 3 | 4 | 1 | 1 | 0 | 2 | 3 | 9 | 10 | 19 | 35 | 32 | 1 | 0 | 0 | 4 | 5 | 3 |
SpindlePole | 4 | – | 0 | 7 | 21 | 25 | 62 | 48 | 15 | 29 | 20 | 35 | 7 | 7 | 12 | 24 | 31 | 25 | 9 | 0 | 2 | 5 | 17 | 21 |
Vac/Vac Membrane | 0 | – | 1 | 1 | 19 | 8 | 8 | 16 | 3 | 2 | 4 | 10 | 11 | 16 | 16 | 52 | 41 | 44 | 6 | 2 | 1 | 10 | 9 | 26 |
Unique Cell Count | 126 | 28 | 268 | 524 | 374 | 739 | 530 | 296 | 400 | 347 | 418 | 291 | 419 | 494 | 202 | 221 | 202 | 136 | 45 | 61 | 197 | 302 | 417 | |
Labelled Cell Count | 146 | 31 | 286 | 560 | 423 | 877 | 627 | 373 | 473 | 422 | 490 | 313 | 443 | 511 | 250 | 274 | 255 | 136 | 45 | 61 | 197 | 302 | 417 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | – | 5.3 | 4.6 | 4.9 | 4.1 | 3.5 | 4.1 | 3.5 | 3.6 | 3.3 | 3.6 | 5.0 | 5.4 | 5.7 | 6.6 | 6.9 | 6.8 | 5.0 | 6.8 | 6.0 |
Std Deviation (1e-4) | 0.5 | – | 2.1 | 1.6 | 2.0 | 2.5 | 1.4 | 1.5 | 1.7 | 0.9 | 1.5 | 1.1 | 1.2 | 1.7 | 4.9 | 1.4 | 1.8 | 1.9 | 2.2 | 10.3 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.11 | -0.37 | -0.6 | -0.36 | -0.59 | -0.58 | -0.68 | -0.56 | -0.08 | 0.04 | 0.1 | 0.32 | 0.37 | 0.35 | -0.09 | 0.35 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.6 | -1.8 | -2.4 | -2.8 | -3.3 | -3.1 | -4.1 | -3.3 | -0.2 | -0.3 | 0.2 | -9.2 | -8.7 | -8.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7741 | -0.0367 | -0.5378 | -0.2496 | -0.3701 | 0.2535 | -0.425 | 0.2108 | 0.0145 | -0.051 | 0.2097 | 0.1759 | -0.9722 | -0.5766 | -0.7197 | -0.6414 | -0.6056 | -0.9698 |
Actin | 0.0395 | 0.016 | 0.003 | 0.0033 | 0.0026 | 0.019 | 0.0192 | 0.0047 | 0.0175 | 0.0103 | 0.0036 | 0.0096 | 0.0157 | 0.0159 | 0.0262 | 0.0045 | 0.0146 | 0.0133 |
Bud | 0.0013 | 0.0047 | 0.0014 | 0.0047 | 0.0008 | 0.0013 | 0.0013 | 0.0027 | 0.0019 | 0.0014 | 0.0036 | 0.0005 | 0.0012 | 0.0036 | 0.0029 | 0.0022 | 0.006 | 0.0037 |
Bud Neck | 0.0029 | 0.0017 | 0.0074 | 0.0003 | 0.0015 | 0.0035 | 0.0074 | 0.0014 | 0.0023 | 0.0005 | 0.0006 | 0.0096 | 0.0008 | 0.0014 | 0.0121 | 0.0026 | 0.0033 | 0.0032 |
Bud Periphery | 0.0038 | 0.0098 | 0.0034 | 0.0108 | 0.0049 | 0.0015 | 0.0083 | 0.0055 | 0.0044 | 0.0035 | 0.0127 | 0.0017 | 0.005 | 0.0095 | 0.0075 | 0.0064 | 0.0082 | 0.0119 |
Bud Site | 0.0042 | 0.0248 | 0.0164 | 0.0007 | 0.0038 | 0.001 | 0.0075 | 0.0235 | 0.0097 | 0.0011 | 0.0013 | 0.0006 | 0.0055 | 0.0199 | 0.0176 | 0.0034 | 0.0158 | 0.0014 |
Cell Periphery | 0.0023 | 0.0021 | 0.0015 | 0.0004 | 0.0011 | 0.0007 | 0.0009 | 0.0029 | 0.0019 | 0.0006 | 0.0004 | 0.0006 | 0.0009 | 0.0023 | 0.0015 | 0.0032 | 0.001 | 0.0008 |
Cytoplasm | 0.0452 | 0.0747 | 0.0552 | 0.0226 | 0.0198 | 0.0968 | 0.0657 | 0.0882 | 0.0989 | 0.0661 | 0.1348 | 0.1056 | 0.047 | 0.0793 | 0.0895 | 0.0676 | 0.1236 | 0.0825 |
Cytoplasmic Foci | 0.0777 | 0.0781 | 0.1021 | 0.0375 | 0.0776 | 0.0872 | 0.0695 | 0.0786 | 0.0691 | 0.0675 | 0.0662 | 0.101 | 0.0933 | 0.0733 | 0.08 | 0.1087 | 0.0601 | 0.1304 |
Eisosomes | 0.001 | 0.001 | 0.0007 | 0.0004 | 0.0007 | 0.0005 | 0.0011 | 0.0011 | 0.001 | 0.0013 | 0.0003 | 0.0003 | 0.0011 | 0.0017 | 0.0007 | 0.0025 | 0.0008 | 0.0004 |
Endoplasmic Reticulum | 0.0327 | 0.0213 | 0.0022 | 0.0028 | 0.0021 | 0.0262 | 0.0044 | 0.0096 | 0.005 | 0.0056 | 0.003 | 0.0031 | 0.0079 | 0.0059 | 0.0052 | 0.0046 | 0.0072 | 0.0034 |
Endosome | 0.0495 | 0.0674 | 0.0406 | 0.0512 | 0.0674 | 0.1108 | 0.076 | 0.0831 | 0.0573 | 0.102 | 0.0745 | 0.0814 | 0.107 | 0.0819 | 0.0742 | 0.0566 | 0.1142 | 0.0895 |
Golgi | 0.0212 | 0.0211 | 0.0141 | 0.0129 | 0.0216 | 0.0371 | 0.0226 | 0.0228 | 0.0211 | 0.0276 | 0.0125 | 0.018 | 0.0311 | 0.024 | 0.0338 | 0.0175 | 0.0282 | 0.0581 |
Lipid Particles | 0.0466 | 0.0208 | 0.0178 | 0.0689 | 0.0715 | 0.0374 | 0.0626 | 0.0171 | 0.0076 | 0.0113 | 0.0109 | 0.014 | 0.0506 | 0.0214 | 0.0232 | 0.0285 | 0.0129 | 0.0342 |
Mitochondria | 0.1544 | 0.1552 | 0.2667 | 0.3658 | 0.1888 | 0.1684 | 0.2017 | 0.1845 | 0.1895 | 0.2127 | 0.2899 | 0.2791 | 0.276 | 0.1679 | 0.2935 | 0.2638 | 0.4884 | 0.3496 |
None | 0.4183 | 0.4321 | 0.4169 | 0.3814 | 0.3877 | 0.3299 | 0.3889 | 0.4155 | 0.4584 | 0.4411 | 0.3462 | 0.3264 | 0.2858 | 0.4221 | 0.2731 | 0.3612 | 0.0563 | 0.1648 |
Nuclear Periphery | 0.0242 | 0.0033 | 0.0008 | 0.0036 | 0.0011 | 0.024 | 0.0025 | 0.0021 | 0.0021 | 0.0017 | 0.001 | 0.0011 | 0.0054 | 0.0052 | 0.0038 | 0.0016 | 0.0151 | 0.0011 |
Nucleolus | 0.0021 | 0.002 | 0.0015 | 0.0009 | 0.0014 | 0.0009 | 0.0046 | 0.0034 | 0.0007 | 0.0004 | 0.0006 | 0.0003 | 0.0007 | 0.0014 | 0.0011 | 0.0011 | 0.0011 | 0.0006 |
Nucleus | 0.0222 | 0.0026 | 0.0013 | 0.0018 | 0.0009 | 0.0045 | 0.0026 | 0.0042 | 0.0015 | 0.0011 | 0.0011 | 0.0009 | 0.001 | 0.0028 | 0.0024 | 0.0014 | 0.0033 | 0.001 |
Peroxisomes | 0.0296 | 0.0433 | 0.0317 | 0.0154 | 0.1323 | 0.0331 | 0.0341 | 0.0267 | 0.0395 | 0.0354 | 0.0252 | 0.0332 | 0.0478 | 0.0327 | 0.0328 | 0.053 | 0.0122 | 0.0396 |
Punctate Nuclear | 0.0076 | 0.0035 | 0.0018 | 0.0032 | 0.0008 | 0.0048 | 0.0056 | 0.0041 | 0.0013 | 0.0011 | 0.0014 | 0.0012 | 0.0028 | 0.0011 | 0.008 | 0.0012 | 0.0025 | 0.0016 |
Vacuole | 0.0102 | 0.0117 | 0.0109 | 0.0069 | 0.0077 | 0.0071 | 0.0104 | 0.0143 | 0.0066 | 0.0051 | 0.0076 | 0.0078 | 0.0095 | 0.0225 | 0.0075 | 0.0059 | 0.0179 | 0.0051 |
Vacuole Periphery | 0.0034 | 0.003 | 0.0024 | 0.0047 | 0.0036 | 0.0044 | 0.0031 | 0.0038 | 0.0029 | 0.0023 | 0.0025 | 0.0038 | 0.0041 | 0.0042 | 0.0034 | 0.0025 | 0.0072 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.9747 | 16.9153 | 17.2589 | 25.9781 | 24.5335 | 21.2758 | 34.2062 | 28.4979 | 32.7641 | 29.6771 |
Translational Efficiency | 0.7986 | 1.5 | 0.9594 | 0.8297 | 0.843 | 1.312 | 0.793 | 0.9762 | 1.1026 | 1.0175 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
593 | 1284 | 2571 | 1672 | 1513 | 2017 | 226 | 152 | 2106 | 3301 | 2797 | 1824 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 641.59 | 722.05 | 761.31 | 813.63 | 612.07 | 718.18 | 875.67 | 891.63 | 620.38 | 719.69 | 770.55 | 820.13 |
Standard Deviation | 85.36 | 93.04 | 99.85 | 118.24 | 74.80 | 87.06 | 103.83 | 141.39 | 79.05 | 89.45 | 104.91 | 122.25 |
Intensity Change Log 2 | — | 0.170447 | 0.246832 | 0.342721 | — | 0.230649 | 0.516691 | 0.542749 | — | 0.200153 | 0.384891 | 0.443846 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000394 | 0.001028 | 0.008043 | 0.006886 | 0.000817 | 0.000988 | 0.007320 | 0.008702 | 0.000698 | 0.001004 | 0.007985 | 0.007037 |
Bud Neck | 0.036178 | 0.072765 | 0.050133 | 0.029475 | 0.048060 | 0.073076 | 0.014680 | 0.014284 | 0.044714 | 0.072955 | 0.047268 | 0.028209 |
Bud Site | 0.012425 | 0.048635 | 0.075676 | 0.089582 | 0.011303 | 0.027863 | 0.057216 | 0.061662 | 0.011619 | 0.035943 | 0.074184 | 0.087255 |
Cell Periphery | 0.000273 | 0.000328 | 0.000393 | 0.000532 | 0.000255 | 0.000177 | 0.000592 | 0.000264 | 0.000260 | 0.000236 | 0.000409 | 0.000509 |
Cytoplasm | 0.309533* | 0.217730* | 0.146519* | 0.162335* | 0.295640* | 0.300541* | 0.166192* | 0.208096* | 0.299552* | 0.268330* | 0.148108* | 0.166148* |
Cytoplasmic Foci | 0.279107* | 0.257318* | 0.088924 | 0.088962 | 0.298513* | 0.204601* | 0.157434 | 0.104379 | 0.293049* | 0.225106* | 0.094460 | 0.090247 |
Eisosomes | 0.000237 | 0.000181 | 0.000146 | 0.000151 | 0.000278 | 0.000150 | 0.000216 | 0.000151 | 0.000266 | 0.000162 | 0.000152 | 0.000151 |
Endoplasmic Reticulum | 0.001259 | 0.000746 | 0.002966 | 0.004621 | 0.003261 | 0.000830 | 0.004432 | 0.003659 | 0.002697 | 0.000797 | 0.003085 | 0.004541 |
Endosome | 0.032695 | 0.052188 | 0.055514 | 0.048369 | 0.033179 | 0.038112 | 0.061818 | 0.052967 | 0.033043 | 0.043587 | 0.056023 | 0.048752 |
Golgi | 0.010070 | 0.025288 | 0.034668 | 0.036092 | 0.013308 | 0.015988 | 0.056252 | 0.033429 | 0.012397 | 0.019605 | 0.036412 | 0.035870 |
Lipid Particles | 0.021054 | 0.007803 | 0.006893 | 0.007521 | 0.029097 | 0.007467 | 0.007244 | 0.003254 | 0.026832 | 0.007598 | 0.006921 | 0.007165 |
Mitochondria | 0.036642 | 0.091639 | 0.200972* | 0.152336* | 0.055319 | 0.057706 | 0.058680 | 0.041930 | 0.050060 | 0.070905 | 0.189475* | 0.143135* |
Mitotic Spindle | 0.001378 | 0.018126 | 0.063722 | 0.084620 | 0.001489 | 0.014898 | 0.030333 | 0.055876 | 0.001458 | 0.016154 | 0.061024 | 0.082225 |
None | 0.005428 | 0.002096 | 0.001294 | 0.003931 | 0.002966 | 0.002208 | 0.001846 | 0.001236 | 0.003659 | 0.002164 | 0.001338 | 0.003706 |
Nuclear Periphery | 0.000763 | 0.000275 | 0.001271 | 0.001353 | 0.000805 | 0.000639 | 0.000794 | 0.000919 | 0.000794 | 0.000497 | 0.001233 | 0.001317 |
Nuclear Periphery Foci | 0.000434 | 0.000300 | 0.000582 | 0.001361 | 0.000412 | 0.000295 | 0.001902 | 0.000938 | 0.000419 | 0.000297 | 0.000688 | 0.001326 |
Nucleolus | 0.002636 | 0.002267 | 0.002241 | 0.002406 | 0.001853 | 0.003140 | 0.001415 | 0.000653 | 0.002074 | 0.002801 | 0.002174 | 0.002260 |
Nucleus | 0.157472* | 0.060972 | 0.076465 | 0.100168 | 0.132071* | 0.121272 | 0.176372* | 0.114239 | 0.139223* | 0.097817 | 0.084537 | 0.101340 |
Peroxisomes | 0.042714 | 0.059124 | 0.073791 | 0.045859 | 0.023104 | 0.038414 | 0.079878 | 0.097067 | 0.028625 | 0.046470 | 0.074283 | 0.050126 |
Vacuole | 0.046626 | 0.075425 | 0.101239 | 0.124207 | 0.044043 | 0.086781 | 0.104867 | 0.180936 | 0.044771 | 0.082364 | 0.101532 | 0.128934 |
Vacuole Periphery | 0.002681 | 0.005768 | 0.008549 | 0.009235 | 0.004225 | 0.004855 | 0.010518 | 0.015360 | 0.003790 | 0.005210 | 0.008709 | 0.009745 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.04 | -9.13 | -8.59 | -7.52 | 1.79 | -0.59 | -2.80 | -2.60 | -2.50 | 0.86 | -1.54 | -9.05 | -8.31 | -7.89 | 1.94 |
Bud Neck | -6.72 | -3.33 | 1.90 | 10.77 | 8.25 | -5.65 | 8.85 | 7.17 | 12.59 | -0.38 | -8.09 | -0.92 | 6.05 | 15.95 | 8.12 |
Bud Site | -9.94 | -16.32 | -15.03 | -6.89 | -1.31 | -9.01 | -5.21 | -3.43 | -1.32 | 1.28 | -13.98 | -20.78 | -17.58 | -10.73 | -1.00 |
Cell Periphery | -0.69 | -1.96 | -2.68 | -1.90 | -1.41 | 2.84 | -2.81 | 1.38 | -0.42 | 3.27 | 0.87 | -4.02 | -3.47 | -4.00 | -0.81 |
Cytoplasm | 6.88 | 12.92 | 10.30 | 4.37 | -3.69 | -0.58 | 8.07 | 4.19 | 4.47 | -1.53 | 4.13 | 20.33 | 14.89 | 12.08 | -3.98 |
Cytoplasmic Foci | 1.95 | 20.76 | 21.04 | 25.62 | 1.48 | 12.65 | 11.92 | 21.01 | 12.99 | 5.72 | 11.04 | 36.43 | 37.27 | 30.18 | 3.12 |
Eisosomes | 2.97 | 5.07 | 4.99 | 3.36 | -0.13 | 6.82 | 1.74 | 6.31 | 0.83 | 3.43 | 7.31 | 8.25 | 8.70 | 1.97 | 0.97 |
Endoplasmic Reticulum | 1.75 | -4.76 | -5.26 | -6.54 | -2.66 | 4.59 | -2.24 | -0.81 | -3.95 | 1.03 | 4.88 | -1.21 | -3.05 | -6.88 | -2.51 |
Endosome | -4.77 | -5.53 | -2.36 | 3.29 | 4.28 | -1.81 | -2.74 | 1.24 | 2.08 | 3.06 | -4.71 | -8.00 | -2.84 | 1.35 | 4.96 |
Golgi | -5.78 | -9.25 | -6.80 | -1.55 | 1.34 | -1.55 | -3.80 | -0.05 | 0.35 | 3.12 | -5.10 | -11.12 | -6.89 | -3.74 | 2.26 |
Lipid Particles | 5.58 | 5.97 | 5.81 | 0.73 | -0.16 | 11.06 | 9.80 | 13.37 | 7.21 | 4.21 | 12.35 | 12.41 | 12.20 | 0.99 | 0.33 |
Mitochondria | -7.37 | -20.77 | -10.88 | -4.25 | 8.18 | -0.32 | 0.67 | 4.20 | 4.47 | 2.33 | -4.78 | -21.78 | -9.95 | -7.06 | 8.40 |
Mitotic Spindle | -6.98 | -20.15* | -17.29 | -11.84 | -2.60 | -7.54 | -4.27 | -2.68 | -1.23 | -0.04 | -10.26 | -20.59 | -17.45 | -13.11 | -2.53 |
None | 2.48 | 3.10 | 1.29 | -1.99 | -3.30 | 1.67 | 1.85 | 3.12 | 2.26 | 0.93 | 2.97 | 4.59 | 0.31 | -1.98 | -3.23 |
Nuclear Periphery | 4.16 | -3.68 | -5.73 | -11.67 | -2.07 | 1.17 | -1.21 | -4.63 | -5.60 | -3.69 | 3.01 | -4.06 | -6.77 | -9.89 | -2.47 |
Nuclear Periphery Foci | 1.10 | -1.52 | -5.46 | -6.92 | -5.07 | 1.52 | -5.95 | -4.89 | -5.37 | 2.02 | 1.87 | -4.57 | -6.80 | -7.70 | -4.55 |
Nucleolus | 0.52 | 0.72 | 0.53 | -0.01 | -0.22 | -2.46 | 1.04 | 1.64 | 3.32 | 0.75 | -2.10 | -0.52 | -0.55 | 1.61 | -0.10 |
Nucleus | 10.33 | 8.19 | 3.69 | -9.61 | -6.38 | 1.57 | -3.54 | -2.07 | -2.57 | 0.84 | 8.19 | 9.02 | 2.15 | -4.69 | -5.71 |
Peroxisomes | -2.87 | -5.26 | 0.53 | 4.61 | 7.95 | -5.15 | -4.36 | -1.63 | -0.41 | 1.71 | -6.65 | -12.34 | -3.58 | 1.90 | 8.05 |
Vacuole | -6.70 | -13.03 | -18.73 | -13.89 | -8.85 | -11.97 | -7.79 | -12.25 | -10.42 | -7.68 | -13.76 | -17.83 | -23.62 | -16.22 | -10.87 |
Vacuole Periphery | -5.77 | -8.63 | -7.01 | -2.70 | 0.71 | -1.08 | -1.70 | -0.68 | -0.50 | 0.58 | -3.39 | -7.24 | -5.42 | -3.56 | 0.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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