Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0.1 0.16 0.21 0.13 0.1 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0 0.12 0.19 0.22 0.5 0.52 0.52 0.57 0.67 0.62 0.61 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.92 0.9 0.9 0.89 0.81 0.76 0.72 0.74 0.76 0.71 0.76 0.76 0.86 0.8 0.76 0.77 0.78 0.76 0.88 0.85 0.81 0.81 0.75 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.11 0.05 0.07 0.13 0.07 0.1 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0
Bud 0 0 0 2 1 0 4 5 3 4 5 4 0 0 0 1 0 0 1 4 2 0 2 7
Bud Neck 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 1 0 0 0 0 3 11 9 5 7 12 25 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 19 8 6 15 16 26 24 9 17 10 16 20 36 70 16 13 17 0 4 1 3 1 5
Endoplasmic Reticulum 1 1 1 0 0 2 0 0 0 0 0 0 0 0 0 5 6 5 0 1 0 0 0 0
Endosome 0 4 2 1 2 0 0 0 0 0 0 0 0 1 2 3 2 4 1 5 0 0 6 5
Golgi 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 2 2 0 0 0
Mitochondria 62 13 31 42 68 174 233 237 189 288 191 227 7 3 3 7 0 2 0 3 3 1 1 7
Nucleus 173 432 232 202 250 262 321 341 253 305 232 283 166 185 248 92 100 101 174 432 222 88 186 146
Nuclear Periphery 3 4 0 0 0 2 8 3 3 4 2 4 0 1 1 0 0 1 0 0 0 0 0 1
Nucleolus 3 5 1 0 1 8 19 16 15 29 19 26 0 0 2 1 0 1 0 4 3 2 16 18
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 1 0 0 0 0 0 0 0 1
SpindlePole 1 1 0 0 1 0 0 1 1 2 0 1 1 2 1 0 0 1 5 4 1 0 3 8
Vac/Vac Membrane 1 39 8 5 19 7 11 5 2 4 4 5 3 5 11 11 15 14 9 36 34 7 24 56
Unique Cell Count 189 480 258 226 309 347 444 458 331 428 306 374 193 230 326 120 129 133 198 508 276 109 249 270
Labelled Cell Count 249 520 284 258 358 474 633 642 481 660 475 591 197 235 339 137 137 150 198 508 276 109 249 270


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.9 5.1 4.6 4.6 4.3 3.8 4.0 3.9 3.7 3.8 3.8 5.4 5.3 5.3 7.4 8.3 8.6 4.8 5.6 6.6
Std Deviation (1e-4) 0.8 1.3 1.3 1.1 1.3 1.2 1.1 1.2 0.8 1.2 0.9 1.3 1.0 1.3 1.4 1.8 1.9 1.9 1.2 1.6 1.8
Intensity Change (Log2) – – – -0.13 -0.16 -0.25 -0.41 -0.34 -0.37 -0.45 -0.4 -0.44 0.09 0.06 0.05 0.54 0.7 0.76 -0.07 0.15 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 1.1 0.9 1.6 1.3 -0.3 0.6 0.1 0.8 3.2 4.8 6.5 3.8 2.9 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.2 -4.6 -5.7 -1.9 -4.1 -3.5
Nucleus -0.2 -3.0 -4.5 -5.5 -5.0 -4.3 -5.7 -4.4 -4.5 -1.3 -3.0 -4.3 -3.4 -3.3 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 1.7 -0.8 -0.5 0 0 0 -1.5 -1.5 0 -0.6 0.2 2.5 3.3 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2285 1.2896 0.1366 -0.2878 0.1337 0.7351 3.3378 3.3946 3.3175 2.9582 3.5595 3.4437 4.2386 4.6659 4.3565 4.6145 4.2344 4.7111
Actin 0.0045 0 0.0003 0.0002 0.0135 0.001 0.01 0 0.0074 0.0234 0.002 0.0005 0.001 0 0.0053 0.0006 0 0.0062
Bud 0 0 0.0003 0.0041 0.0001 0.002 0.0007 0 0.004 0.0005 0.0005 0.0001 0.0001 0.0003 0.0001 0.0001 0 0
Bud Neck 0.0003 0.0001 0.0003 0.0008 0.0016 0.001 0.0018 0.0001 0.0065 0.0075 0.0009 0.0012 0.0009 0.0001 0.0009 0.0009 0.0005 0.0007
Bud Periphery 0 0 0.0001 0.0011 0.0002 0.0006 0.0027 0 0.002 0.0009 0.0026 0.0004 0.0001 0.0001 0.0003 0.0003 0 0
Bud Site 0.0002 0 0.0006 0.0016 0.0003 0 0.0013 0 0.0059 0.0037 0.0008 0.0001 0.0002 0.0002 0.0004 0.0004 0 0
Cell Periphery 0 0 0 0.0003 0 0 0.0004 0 0.0009 0.0006 0.0003 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.004 0.0008 0.0059 0.0215 0.0029 0.0039 0.0058 0.0005 0.0116 0.0016 0.0033 0.0006 0.0021 0.0015 0.0054 0.0005 0.0005 0.0028
Cytoplasmic Foci 0.0023 0 0.0005 0.0199 0.0007 0.0003 0.003 0 0.008 0.0048 0.0076 0.0002 0.0002 0.0025 0.0018 0.0001 0 0.0021
Eisosomes 0.0003 0 0.0002 0 0.0005 0.0001 0.0004 0 0.0001 0.0001 0 0 0 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0004 0 0.0004 0.0006 0.0004 0.0003 0.0045 0.0001 0.0019 0.0003 0.0042 0.0004 0.0024 0.0001 0.0013 0.0005 0 0.0001
Endosome 0.0029 0 0.0005 0.0011 0.0014 0.0004 0.0119 0.0001 0.0068 0.0022 0.0335 0.0009 0.0036 0.0001 0.0038 0.0011 0 0.0003
Golgi 0.0011 0 0 0.0001 0.0011 0.0001 0.0041 0 0.0018 0.0011 0.0145 0.0002 0.0002 0 0.0006 0.0001 0 0.0002
Lipid Particles 0.0033 0 0.0002 0.0006 0.0026 0.0001 0.0071 0 0.0029 0.0025 0.0085 0.0009 0.0004 0 0.0013 0.0006 0 0.0001
Mitochondria 0.0007 0.0001 0.0002 0.0032 0.0015 0.0004 0.0195 0.0001 0.0105 0.0006 0.0229 0.001 0.0016 0.0016 0.0004 0.0021 0.0001 0.0001
None 0.0532 0.0288 0.0356 0.043 0.0461 0.0112 0.0051 0.0001 0.0115 0.0003 0.0199 0.0048 0.0014 0.0134 0.0109 0.0014 0 0.0032
Nuclear Periphery 0.0122 0.0052 0.0119 0.0056 0.0033 0.0066 0.0161 0.0042 0.0064 0.0018 0.0159 0.0035 0.0241 0.0047 0.0059 0.0066 0.0009 0.003
Nucleolus 0.0195 0.0087 0.0208 0.0132 0.0198 0.0217 0.0098 0.0086 0.019 0.0379 0.0148 0.0163 0.007 0.0103 0.0046 0.015 0.017 0.0125
Nucleus 0.8502 0.9516 0.88 0.8427 0.8843 0.9355 0.8794 0.9845 0.875 0.8349 0.8207 0.9575 0.9449 0.9605 0.9469 0.9638 0.9772 0.9521
Peroxisomes 0.0015 0 0 0.0002 0.0029 0 0.0054 0 0.0054 0.0198 0.0034 0.0002 0.0001 0.0001 0.0007 0.0002 0 0
Punctate Nuclear 0.0424 0.0045 0.0417 0.0385 0.0165 0.0144 0.0071 0.0012 0.007 0.0508 0.0041 0.0104 0.0074 0.0041 0.0085 0.0039 0.0034 0.0162
Vacuole 0.0005 0.0001 0.0003 0.0013 0.0002 0.0002 0.0026 0.0004 0.0043 0.0042 0.0108 0.0005 0.0013 0.0002 0.0008 0.0009 0.0001 0.0001
Vacuole Periphery 0.0004 0 0.0002 0.0003 0.0002 0.0002 0.0015 0.0001 0.001 0.0004 0.0088 0.0003 0.001 0.0001 0.0002 0.0006 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8607 12.6203 12.3221 12.7836 8.6704 6.6836 16.4542 15.185 13.0136 14.6162
Translational Efficiency 0.691 0.8303 0.83 0.7357 1.1406 1.5668 0.6654 0.8362 0.779 0.8494

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
451 1386 604 1324 1904 2232 112 1083 2355 3618 716 2407

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.50 773.27 941.87 912.11 715.74 776.62 1059.33 926.32 703.63 775.34 960.24 918.50
Standard Deviation 69.96 106.25 119.79 126.02 94.61 107.89 106.19 143.05 93.77 107.28 125.26 134.14
Intensity Change Log 2 — 0.244994 0.529550 0.483230 — 0.117773 0.565645 0.372075 — 0.179845 0.548544 0.426153

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000042 0.000359 0.000779 0.000867 0.000085 0.000378 0.001231 0.000826 0.000076 0.000371 0.000850 0.000849
Bud Neck 0.000571 0.007504 0.012271 0.029997 0.001084 0.007306 0.004095 0.023081 0.000986 0.007382 0.010992 0.026885
Bud Site 0.000092 0.001631 0.001243 0.009800 0.000248 0.002511 0.001214 0.008760 0.000218 0.002174 0.001238 0.009332
Cell Periphery 0.000067 0.000117 0.000057 0.000124 0.000076 0.000125 0.000095 0.000132 0.000074 0.000122 0.000063 0.000128
Cytoplasm 0.015107 0.022856 0.001702 0.012868 0.046124 0.017733 0.001385 0.026037 0.040184 0.019696 0.001652 0.018794
Cytoplasmic Foci 0.002260 0.001943 0.000027 0.002023 0.001767 0.004875 0.000150 0.003976 0.001861 0.003752 0.000046 0.002902
Eisosomes 0.000011 0.000015 0.000027 0.000025 0.000009 0.000020 0.000051 0.000023 0.000010 0.000018 0.000031 0.000024
Endoplasmic Reticulum 0.000614 0.004960 0.005430 0.008023 0.001060 0.004330 0.010527 0.007181 0.000974 0.004571 0.006227 0.007644
Endosome 0.000154 0.000493 0.000161 0.002821 0.000187 0.001849 0.000398 0.005216 0.000181 0.001329 0.000198 0.003899
Golgi 0.000047 0.000516 0.000051 0.001966 0.000038 0.001180 0.000075 0.002317 0.000040 0.000926 0.000055 0.002124
Lipid Particles 0.000486 0.000383 0.000103 0.000373 0.000333 0.001861 0.000368 0.000290 0.000362 0.001295 0.000145 0.000336
Mitochondria 0.002166 0.001189 0.000806 0.003439 0.000358 0.007091 0.000903 0.006560 0.000705 0.004830 0.000821 0.004843
Mitotic Spindle 0.001814 0.001387 0.000154 0.025009 0.000265 0.005980 0.000196 0.040766 0.000562 0.004221 0.000160 0.032098
None 0.010291 0.007694 0.001384 0.003873 0.014811 0.006101 0.001053 0.010722 0.013945 0.006711 0.001333 0.006955
Nuclear Periphery 0.000373 0.001392 0.000278 0.004461 0.000457 0.002468 0.000516 0.002638 0.000441 0.002056 0.000315 0.003641
Nuclear Periphery Foci 0.000084 0.000368 0.000063 0.002266 0.000212 0.000771 0.000324 0.001129 0.000188 0.000617 0.000104 0.001754
Nucleolus 0.008930 0.005499 0.004779 0.003075 0.003715 0.006868 0.005190 0.004147 0.004714 0.006343 0.004843 0.003557
Nucleus 0.956332* 0.939426* 0.968901* 0.878478* 0.928466* 0.921242* 0.965830* 0.838086* 0.933802* 0.928208* 0.968420* 0.860304*
Peroxisomes 0.000123 0.000732 0.000204 0.000425 0.000120 0.002249 0.000284 0.001095 0.000121 0.001668 0.000217 0.000727
Vacuole 0.000273 0.001328 0.001484 0.008455 0.000511 0.004102 0.006007 0.012934 0.000466 0.003039 0.002192 0.010471
Vacuole Periphery 0.000162 0.000207 0.000097 0.001633 0.000073 0.000960 0.000108 0.004082 0.000090 0.000672 0.000099 0.002735

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.68 -8.39 -14.77 -8.51 -1.33 -4.53 -8.04 -18.79 -7.87 2.38 -6.57 -10.09 -22.04 -10.73 -0.73
Bud Neck -8.30 -6.38 -14.96 -10.92 -6.67 -9.83 -8.26 -13.40 -9.43 -10.40 -12.64 -6.59 -19.77 -14.47 -7.85
Bud Site -4.67 -5.14 -6.12 -4.70 -4.90 -6.24 -4.37 -6.61 -4.12 -5.04 -7.58 -5.47 -8.72 -6.01 -6.93
Cell Periphery -2.55 0.42 -3.03 -0.07 -5.06 -2.82 -1.36 -4.43 -0.11 -0.69 -3.68 0.60 -5.19 0.03 -5.66
Cytoplasm -2.39 5.05 1.14 5.00 -8.84 9.37 16.08 6.15 -2.46 -9.99 8.06 16.69 8.85 1.58 -12.91
Cytoplasmic Foci 0.29 2.46 1.32 1.34 -2.08 -4.03 4.76 0.46 3.82 -2.46 -3.16 5.72 1.56 4.40 -3.36
Eisosomes -3.01 -6.94 -13.09 -10.73 -1.59 -7.81 -6.85 -17.19 -7.30 3.37 -8.56 -9.95 -21.77 -11.22 -0.16
Endoplasmic Reticulum -17.44 -11.56 -18.56 -9.16 -6.16 -12.06 -10.78 -19.58 -9.92 2.33 -18.45 -13.82 -25.82 -13.29 -5.43
Endosome -4.12 -0.76 -3.91 -2.97 -3.85 -6.05 -3.48 -4.24 -1.12 -3.64 -6.62 -1.37 -5.70 -1.92 -5.58
Golgi -1.59 -0.55 -1.76 -0.70 -1.73 -3.59 -4.10 -3.04 0.44 -2.83 -3.91 -3.59 -2.96 -0.05 -2.88
Lipid Particles 0.94 3.33 0.93 0.07 -3.87 -4.27 -1.02 -1.67 4.03 0.34 -4.15 4.34 -0.52 4.03 -4.83
Mitochondria 0.50 0.70 -0.69 -5.83 -6.86 -6.93 -6.55 -5.08 2.00 -4.02 -5.95 -0.60 -5.88 1.25 -7.05
Mitotic Spindle 0.28 0.79 -4.71 -5.82 -6.28 -5.01 -0.94 -7.79 -6.06 -7.39 -4.36 0.13 -9.76 -7.94 -9.86
None 1.50 5.46 3.86 3.64 -6.36 5.34 9.98 3.70 -0.94 -4.73 5.53 11.05 6.41 1.20 -6.47
Nuclear Periphery -7.92 1.17 -6.08 -4.43 -6.18 -7.33 -2.75 -12.00 -3.34 -10.31 -9.09 1.10 -9.60 -4.82 -9.77
Nuclear Periphery Foci -3.17 0.07 -2.85 -2.13 -2.86 -3.59 -1.02 -3.93 -0.71 -1.04 -4.05 0.63 -3.81 -1.90 -3.94
Nucleolus 2.21 2.32 3.72 3.26 2.20 -4.84 -1.02 -1.24 3.39 0.43 -3.07 -0.45 2.00 4.97 1.81
Nucleus 2.55 -1.59 9.08 8.30 12.97 1.61 -4.70 9.71 8.41 12.33 1.52 -7.70 11.79 10.93 18.57
Peroxisomes -2.49 -2.31 -2.56 1.79 -0.58 -4.78 -2.62 -1.29 3.32 -0.66 -5.30 -2.72 -1.85 4.05 -0.96
Vacuole -5.56 -5.99 -9.74 -8.76 -8.31 -6.59 -3.71 -9.61 -7.81 -3.96 -7.29 -5.41 -13.21 -10.74 -10.61
Vacuole Periphery -0.47 0.60 -2.24 -2.18 -2.57 -4.92 -3.38 -3.03 -1.36 -2.93 -5.12 -0.76 -3.92 -1.82 -3.87
Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp31

Prp31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available