Standard name
Human Ortholog
Description (SGD) RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.13 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.07 0.13 0.07 0.09 0.06 0 0.05 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.06 0.08 0 0.22 0.16 0.15 0.08 0.19 0 0 0.19 0.06 0.12 0 0 0 0 0 0
Nucleus 0.11 0.14 0.13 0.05 0.06 0 0.08 0.05 0.08 0.09 0.07 0.06 0.05 0 0.05 0.08 0.07 0.06 0.07 0.13 0.08 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.88 0.88 0.92 0.88 0.92 0.86 0.86 0.83 0.86 0.87 0.85 0.92 0.86 0.8 0.9 0.91 0.9 0.84 0.74 0.78 0.81 0.68 0.58
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.24 0.29 0.33 0.26 0.35 0.29 0.31 0.31 0.45 0 0.07 0 0 0 0 0 0 0.1 0.09 0.15 0.19
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 8 4 4 6 3 2 0 0 0 0 0 0 1 3 1 0 1 4
Bud Neck 0 1 12 3 4 6 9 6 5 10 5 2 4 0 2 0 0 0 0 0 3 2 5 9
Bud Site 0 0 1 0 1 0 17 6 25 15 8 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 2 2 4 8 6 7 10 10 2 3 3 1 10 20 32 13 11 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 3 1 2 1 0 0 0 0 0
Endosome 1 2 5 12 28 46 35 32 22 6 18 33 2 6 12 1 1 4 1 5 3 1 5 9
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0 3 0
Mitochondria 23 10 5 0 5 22 41 15 82 62 50 30 38 4 9 93 26 35 0 2 1 0 3 2
Nucleus 18 38 52 12 25 12 40 18 31 36 22 22 11 10 13 40 27 18 12 38 32 5 6 6
Nuclear Periphery 0 0 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 4
Nucleolus 145 245 359 230 377 316 446 302 307 334 284 322 188 192 201 439 375 260 146 219 331 125 169 156
Peroxisomes 1 0 1 2 2 6 5 3 1 1 2 0 2 2 1 2 1 2 0 0 0 0 2 2
SpindlePole 1 11 42 60 126 112 134 125 107 120 102 171 4 15 11 1 4 3 6 12 44 14 36 50
Vac/Vac Membrane 1 6 1 0 13 1 5 3 3 3 1 2 0 3 5 3 8 5 1 10 4 2 7 11
Unique Cell Count 158 277 406 250 428 344 520 353 371 388 328 377 204 223 251 490 413 290 174 296 425 155 248 268
Labelled Cell Count 192 316 482 329 591 529 750 524 590 598 499 604 259 253 289 598 455 336 174 296 425 155 248 268


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.8 20.5 17.4 14.5 13.1 10.6 9.4 9.5 7.8 7.6 7.4 8.1 18.0 18.4 16.0 36.5 38.7 35.2 20.9 19.3 19.8
Std Deviation (1e-4) 6.1 6.4 4.4 3.7 2.9 2.3 2.1 2.2 2.0 2.0 1.9 1.9 5.7 5.7 5.2 13.9 16.3 15.6 4.7 5.2 5.3
Intensity Change (Log2) – – – -0.26 -0.42 -0.72 -0.89 -0.87 -1.15 -1.2 -1.23 -1.1 0.05 0.08 -0.12 1.07 1.15 1.01 0.27 0.15 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -1.5 -2.1 -1.1 -1.2 -1.1 -1.5 -0.3 -1.3 -2.6 -0.7 0 -1.9 -3.8 -3.5 -3.0
Bud Site 0 0 0 3.3 0 5.0 3.6 0 4.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.2 1.2 1.9 0 0 0 0 0 5.0 6.4 1.8 1.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2.8 3.9 6.6 4.1 5.0 3.6 0.4 3.3 4.9 0 0 2.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 4.6 2.6 9.2 7.5 7.2 4.6 7.9 0 2.0 8.5 3.8 6.1
Nucleus -3.4 -3.5 -4.6 -2.6 -3.7 -2.0 -1.6 -2.7 -3.3 -2.8 -3.4 -3.2 -2.3 -3.0 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 -0.2 1.6 -1.2 -1.2 -2.3 -1.0 -0.8 -1.3 1.4 -0.8 -2.9 0.6 1.1 0.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.7 6.9 7.5 5.9 8.3 6.5 7.2 7.0 11.0 -3.7 -1.5 -2.7 -7.1 -5.8 -4.9
Vacuole 0 3.1 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.1882 15.7235 12.905 14.7151 12.2415 13.0957 17.2055 21.1285 17.63 16.8607 15.2181 17.3646 16.0099 18.5141 16.6381 15.5279 15.8251 15.7277
Actin 0.0453 0 0.0076 0.0076 0 0.0107 0.0066 0 0.0062 0.0015 0.0013 0.0008 0.0003 0 0.001 0.0001 0 0.0093
Bud 0.0008 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0004 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0022 0.0001 0.0003 0.0024 0 0.0003 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002
Bud Periphery 0.001 0 0.0001 0.0002 0 0.0004 0.0006 0 0.0009 0.0001 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0036 0.0002 0.0012 0.0002 0 0.0003 0.0013 0 0.0016 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001
Cell Periphery 0.0004 0 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.0068 0 0.0011 0.0004 0.0001 0.0002 0.0004 0 0.0013 0 0 0 0.0004 0 0.0005 0.0006 0 0.0001
Cytoplasmic Foci 0.0118 0.0015 0.0129 0.0039 0.019 0.0114 0.009 0.0001 0.004 0.0008 0.0042 0.004 0.0065 0.0001 0.0005 0.0008 0.0012 0.0006
Eisosomes 0.0007 0 0.0002 0 0 0.0014 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0152 0 0.001 0.0004 0 0.0012 0.0016 0 0.0006 0.0002 0.0006 0.0001 0.0001 0 0.0004 0.0001 0 0.0001
Endosome 0.0463 0 0.0121 0.0142 0.0001 0.0035 0.0137 0 0.0084 0.0035 0.006 0.0019 0.0039 0 0.0017 0.0001 0 0.0009
Golgi 0.0136 0 0.004 0.0067 0.0001 0.0031 0.0062 0 0.0028 0.0033 0.0051 0.0027 0.0005 0 0.0002 0 0.0002 0.0007
Lipid Particles 0.0217 0.0002 0.0048 0.003 0.0135 0.0147 0.0187 0.0001 0.018 0.0204 0.0451 0.0073 0.0018 0.0001 0.0003 0.0003 0.0183 0.0011
Mitochondria 0.016 0.0001 0.0164 0.0012 0.0019 0.0049 0.0118 0.0002 0.0134 0.0211 0.0302 0.0023 0.0007 0 0.0001 0.0002 0.0004 0.0002
None 0.0102 0 0.0021 0.0002 0.0002 0.0004 0.0011 0 0.002 0.0001 0.0001 0 0.0004 0 0.0002 0.0004 0 0.0002
Nuclear Periphery 0.0509 0 0.0025 0.0007 0 0.0005 0.0123 0 0.0007 0.0018 0.0046 0.0017 0.0002 0 0.0025 0.0013 0.0003 0.0011
Nucleolus 0.6245 0.8584 0.8558 0.8695 0.9053 0.8851 0.8363 0.9008 0.8855 0.874 0.8405 0.9147 0.9099 0.9068 0.9107 0.8767 0.9257 0.9008
Nucleus 0.0968 0.1321 0.0667 0.0847 0.0571 0.0527 0.0497 0.098 0.0448 0.0693 0.0432 0.0597 0.069 0.0894 0.0781 0.1006 0.0506 0.0666
Peroxisomes 0.0053 0 0.0037 0.0025 0.0004 0.0032 0.003 0 0.0014 0.0013 0.0005 0.0002 0.0005 0 0.0001 0 0.0001 0.0002
Punctate Nuclear 0.0121 0.0073 0.005 0.0014 0.001 0.0053 0.0217 0.0004 0.0016 0.0007 0.0126 0.0034 0.0052 0.0031 0.0032 0.0182 0.0026 0.0178
Vacuole 0.0064 0.0001 0.0009 0.0004 0.0003 0.0002 0.002 0.0001 0.0037 0.0004 0.0011 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001
Vacuole Periphery 0.0083 0 0.0013 0.0003 0.0007 0.0002 0.0028 0 0.0019 0.0011 0.0039 0.0006 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 100.5707 100.6069 68.6188 62.6454 70.7855 56.4799 59.3027 45.2841 40.4895 71.8004
Translational Efficiency 1.231 1.2071 1.248 1.0933 1.2881 1.3035 1.1946 1.181 1.0775 1.0704

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Select an organelle or subcellular structure:


Penetrance Data

Penetrance values (percentage of cells with morphologically abnormal structure) at each time point for each of the subcellular structures monitored.

TP0 TP3 TP6 TP9 TP12 TP15 TP18 TP21 TP24
Autophagosome (ATG8) 26.554622 52.302026 69.208633 65.034169 76.000000 62.148760 65.774059 47.280000 47.030498
Septin (CDC11) 21.207430 32.429100 57.939914 64.682927 85.630499 94.528875 87.537994 82.020202 66.485310
Spindle & SPB (DAD2) 23.362832 28.205128 43.201133 52.673797 38.382353 42.562592 51.200000 46.106870 44.821092
P-bodies (DCP2) 36.666667 70.400000 93.500000 95.277778 97.986577 100.000000 94.285714 98.305085 92.428198
Inner Nuclear Membrane (HEH2) 19.307359 41.785150 68.267067 75.934738 85.866477 89.700231 85.358005 84.505788 73.390970
Metacaspase (MCA1) 9.200283 34.574468 66.573971 78.171828 82.284506 83.094714 79.921919 68.061142 54.384411
Plasma Membrane (MID2) 47.176080 30.791789 45.623342 68.526786 79.744136 88.256228 86.242300 87.443946 86.127168
Necrosis Factor (NHP6A) 20.973783 30.477759 54.308943 71.994342 93.852459 99.411765 96.366279 79.090909 76.131687
Nucleolus (NOP10) 27.220077 41.821743 54.221388 71.177945 85.117493 93.041927 93.046033 87.448133 90.845070
Kinetochore (NUF2) 21.043771 17.197452 38.196721 41.243863 40.544218 52.047556 62.622036 68.766404 62.341326
Mitochondria (OM45) 25.742574 36.252189 52.950558 70.114943 83.979328 94.694960 91.309670 89.921466 85.294118
Eisosomes (PIL1) 16.865672 29.331514 51.873536 61.742827 76.825706 95.569620 97.874494 95.104895 92.723264
Proteasome (PRE2) 27.180068 31.727380 68.463303 71.549894 92.224409 91.046278 92.192192 89.445629 78.398314
DNA Damage Foci (RAD52) 16.565164 33.294931 50.457782 50.330396 66.738895 74.777070 79.662921 77.534247 87.773723
Actin Patches (SAC6) 24.031008 28.131868 62.598425 80.000000 90.000000 91.190865 95.379538 97.727273 91.228070
Golgi, Early (SEC21) 29.911504 31.685166 56.187291 59.645233 70.286707 83.622184 92.790698 90.674847 85.330347
Golgi, Late (SEC7) 27.598566 36.063709 54.848801 70.020534 80.997877 92.205128 86.804798 81.911263 76.651481
Endosomes (SNF7) 26.123128 26.576577 36.860215 61.628883 74.371652 76.526403 72.444808 68.166526 59.882747
Endoplasmic Reticulum (SPF1) 36.893204 41.078306 73.913043 74.141414 68.945312 66.579407 66.079892 40.747493 47.759104
Lipid Droplets (TGL3) 9.255178 8.851224 18.342735 28.078299 39.398562 50.940376 52.960449 46.133333 47.661958
Vacuole (VPH1) 54.149378 31.015038 52.889945 72.727273 91.785472 92.034139 85.208776 87.392170 81.674208

Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1298 760 136 1266 1627 1926 178 2588 2925 2686 314 3854

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1303.51 1751.73 2293.94 2767.25 1443.19 1707.18 2473.70 2887.30 1381.21 1719.79 2395.84 2847.86
Standard Deviation 268.23 400.07 462.96 726.86 328.10 377.42 448.44 751.46 310.84 384.49 463.43 745.60
Intensity Change Log 2 — 0.426379 0.815426 1.086051 — 0.242354 0.777409 1.000460 — 0.332618 0.795576 1.041713

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000160 0.000253 0.000322 0.000473 0.000209 0.000256 0.000256 0.000416 0.000187 0.000255 0.000285 0.000435
Bud Neck 0.001148 0.001428 0.003770 0.005528 0.001050 0.001620 0.002968 0.003067 0.001093 0.001566 0.003315 0.003876
Bud Site 0.000498 0.000394 0.001272 0.001537 0.000333 0.000373 0.000984 0.000655 0.000406 0.000379 0.001109 0.000945
Cell Periphery 0.000029 0.000016 0.000023 0.000076 0.000021 0.000026 0.000020 0.000055 0.000024 0.000023 0.000021 0.000062
Cytoplasm 0.000149 0.000482 0.000047 0.000450 0.000229 0.000165 0.000062 0.000239 0.000194 0.000254 0.000055 0.000308
Cytoplasmic Foci 0.005311 0.003847 0.010746 0.009037 0.004839 0.002562 0.008566 0.002935 0.005048 0.002926 0.009510 0.004939
Eisosomes 0.000253 0.000221 0.000196 0.000195 0.000339 0.000350 0.000157 0.000378 0.000301 0.000313 0.000174 0.000318
Endoplasmic Reticulum 0.000240 0.000277 0.000124 0.000122 0.000220 0.000167 0.000070 0.000109 0.000229 0.000198 0.000093 0.000113
Endosome 0.000437 0.000118 0.000036 0.000391 0.000056 0.000119 0.000025 0.000100 0.000225 0.000119 0.000030 0.000196
Golgi 0.000305 0.000256 0.000625 0.001381 0.000265 0.000299 0.000345 0.000257 0.000283 0.000287 0.000466 0.000626
Lipid Particles 0.000090 0.000265 0.000033 0.000065 0.000048 0.000092 0.000014 0.000016 0.000067 0.000141 0.000022 0.000032
Mitochondria 0.001019 0.000731 0.001289 0.003777 0.000825 0.001254 0.001057 0.001936 0.000911 0.001106 0.001158 0.002541
Mitotic Spindle 0.004091 0.017076 0.016646 0.022305 0.003892 0.006109 0.016578 0.004258 0.003980 0.009212 0.016607 0.010186
None 0.000596 0.000325 0.000314 0.000636 0.000696 0.000430 0.000297 0.000506 0.000652 0.000400 0.000304 0.000549
Nuclear Periphery 0.000063 0.000113 0.000131 0.000838 0.000068 0.000297 0.000100 0.000221 0.000066 0.000245 0.000113 0.000424
Nuclear Periphery Foci 0.001557 0.000541 0.000157 0.000283 0.000685 0.000313 0.000054 0.000118 0.001072 0.000377 0.000099 0.000172
Nucleolus 0.700641* 0.670865* 0.659401* 0.719514* 0.698767* 0.693797* 0.705949* 0.700009* 0.699599* 0.687308* 0.685788* 0.706417*
Nucleus 0.167861* 0.180055* 0.110184 0.159533* 0.182121* 0.212096* 0.135542* 0.235626* 0.175793* 0.203030* 0.124559 0.210630*
Peroxisomes 0.114546* 0.121335* 0.193396* 0.069754 0.104403* 0.078456 0.125997* 0.046045 0.108904* 0.090589 0.155189* 0.053833
Vacuole 0.000651 0.001013 0.000664 0.001484 0.000550 0.000641 0.000390 0.001051 0.000595 0.000747 0.000509 0.001193
Vacuole Periphery 0.000356 0.000390 0.000623 0.002621 0.000384 0.000578 0.000570 0.002002 0.000372 0.000525 0.000593 0.002205

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.06 -6.67 -15.16 -6.05 -5.06 -2.86 -1.94 -12.65 -9.53 -5.43 -4.86 -5.46 -19.83 -11.33 -7.11
Bud Neck -1.50 -6.89 -10.47 -9.26 -3.78 -3.06 -5.21 -8.90 -5.58 -0.37 -3.22 -8.37 -13.48 -10.20 -2.21
Bud Site 0.46 -4.75 -6.23 -7.66 -1.45 -0.09 -3.75 -3.40 -3.36 1.71 0.37 -5.92 -6.39 -7.49 1.01
Cell Periphery 1.43 0.44 -4.65 -6.47 -5.71 -0.86 -0.06 -4.19 -2.92 -3.47 -0.00 0.24 -5.77 -5.30 -5.78
Cytoplasm -0.90 2.82 -3.31 0.17 -4.17 0.59 2.32 -0.13 -0.76 -3.47 -0.57 3.02 -2.19 -0.28 -5.50
Cytoplasmic Foci 2.02 -3.51 -3.82 -4.95 1.17 3.16 -1.47 2.44 -1.06 2.42 4.12 -3.15 -0.17 -4.48 3.09
Eisosomes 1.30 2.94 3.53 1.12 0.48 -0.33 8.58 -0.82 -0.52 -5.32 -0.52 8.75 -0.46 -0.03 -4.92
Endoplasmic Reticulum -0.51 3.20 3.02 1.73 -0.34 3.15 10.71 7.59 4.17 -4.63 0.85 7.15 6.11 2.89 -2.27
Endosome 0.80 1.08 0.14 -1.11 -1.68 -1.00 1.90 -0.69 0.27 -1.29 0.58 1.20 0.19 -0.69 -2.09
Golgi 0.71 -4.55 -1.35 -1.42 -0.82 -0.20 -2.00 0.17 0.27 1.34 0.05 -4.73 -1.25 -1.19 -0.37
Lipid Particles -0.94 1.45 0.78 1.21 -1.25 -0.90 2.56 2.48 1.94 -0.52 -1.10 2.33 1.90 1.99 -1.02
Mitochondria 0.59 -0.58 -3.03 -3.89 -3.16 -1.88 -1.72 -7.72 -2.73 -4.51 -0.76 -1.17 -4.80 -4.60 -4.83
Mitotic Spindle -3.48 -3.18 -6.94 -1.65 -1.01 -1.60 -2.08 -0.82 0.97 1.95 -3.63 -3.36 -6.08 -1.30 1.61
None 3.33 3.53 -0.92 -5.79 -6.17 2.02 3.23 1.46 -1.23 -4.41 2.96 4.52 0.98 -3.26 -7.27
Nuclear Periphery -1.95 -3.65 -3.24 -3.00 -2.92 -1.40 -1.77 -6.52 0.39 -4.09 -1.53 -3.61 -4.54 -1.31 -3.83
Nuclear Periphery Foci 1.96 3.03 2.78 1.74 -2.06 2.05 3.58 3.20 3.83 -5.12 2.90 4.26 3.95 3.43 -3.16
Nucleolus 3.36 3.37 -0.96 -3.89 -3.77 0.69 -0.05 0.11 -0.58 0.10 2.36 2.16 -0.37 -2.62 -2.33
Nucleus -1.45 4.98 0.46 1.73 -4.53 -4.73 4.06 -8.41 -3.82 -8.81 -5.48 6.25 -7.57 -1.88 -10.48
Peroxisomes -1.24 -6.61 10.95 10.67 10.35 7.58 -3.03 19.37 13.63 10.33 6.27 -6.75 22.58 16.46 14.43
Vacuole -0.66 -0.74 -3.36 -0.47 -2.90 -0.66 2.01 -2.32 -1.74 -3.23 -0.81 0.53 -3.65 -1.48 -3.98
Vacuole Periphery -1.21 -6.05 -4.10 -4.02 -3.53 -3.17 -3.60 -5.90 -5.07 -5.13 -3.49 -6.42 -7.11 -6.41 -6.13
Standard name
Human Ortholog
Description (SGD) RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
Localization
Cell Percentages nucleolus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Prp43

Prp43


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp43-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available