Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0.06 0.06
Bud 0 0 0 0 0 0 0 0.05 0.07 0.05 0.1 0.06 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0.06 0.06 0 0 0.06 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.71 0.83 0.86 0.9 0.69 0.56 0.53 0.42 0.49 0.3 0.28 0.95 0.97 0.98 0.55 0.54 0.54 0.2 0.22 0.24 0.42 0.34 0.39
Endoplasmic Reticulum 0.08 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.17 0.12 0.08 0.1 0 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.66 0.21 0 0.05 0 0.26 0.47 0.36 0.57 0.51 0.74 0.69 0 0 0 0.15 0.07 0.06 0.54 0.48 0.51 0.36 0.35 0.28
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.1 0.05 0.09 0.09 0.15 0.1 0.08 0.06 0.16 0 0 0 0.15 0.15 0.1 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 3 0 0 0 0 2 0 0 0 0 3 1 1 2 2 2 1 3 4 4 18 18
Bud 0 2 1 0 3 8 18 14 20 14 12 13 2 0 0 1 7 2 0 0 0 2 4 10
Bud Neck 0 0 6 9 14 8 16 18 9 15 4 9 8 3 2 1 3 0 0 3 1 0 4 7
Bud Site 0 0 0 0 0 0 6 4 5 1 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 1 0 0 0 0 2 0 0 0 3 1 2 2 3 0 0 0 1 4 5
Cytoplasm 20 50 49 134 200 172 224 148 129 138 38 59 264 301 378 11 44 44 13 24 27 64 108 120
Endoplasmic Reticulum 4 7 4 0 0 0 0 0 1 1 0 1 0 3 1 1 1 1 0 1 1 0 0 4
Endosome 0 0 0 0 0 4 1 2 2 1 0 0 1 4 3 0 9 14 8 8 11 5 19 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 1 0 1 1 1
Mitochondria 33 15 2 8 8 65 187 100 174 144 93 148 0 1 1 3 6 5 35 53 59 54 110 85
Nucleus 0 1 0 0 1 1 2 4 4 4 3 3 1 0 0 1 0 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0
Peroxisomes 0 0 0 1 0 4 1 0 2 2 1 1 0 0 3 0 1 3 0 0 0 1 2 1
SpindlePole 1 1 2 15 11 22 38 41 31 22 7 34 6 3 6 3 12 8 1 3 3 6 20 18
Vac/Vac Membrane 0 0 0 0 2 2 4 4 1 1 0 6 1 0 1 0 5 7 2 8 1 4 8 8
Unique Cell Count 50 70 59 156 223 250 401 280 305 283 125 213 278 311 384 20 81 82 67 111 117 152 316 310
Labelled Cell Count 59 79 67 168 240 286 497 338 378 345 162 279 287 319 397 25 93 92 67 111 117 152 316 310


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.9 4.3 4.4 4.3 3.9 3.3 3.8 3.1 3.3 3.2 3.2 4.7 5.0 5.1 5.7 5.6 5.7 4.6 5.0 5.5
Std Deviation (1e-4) 0.5 0.5 0.7 1.7 1.4 2.0 1.4 1.5 1.4 1.1 1.5 0.6 1.0 1.1 1.1 1.3 1.6 1.9 1.1 1.8 1.8
Intensity Change (Log2) – – – 0.03 0.02 -0.15 -0.37 -0.18 -0.45 -0.39 -0.41 -0.43 0.12 0.21 0.24 0.41 0.38 0.41 0.09 0.22 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.346 -1.0374 -1.0506 -1.0975 -1.468 -1.017 -1.0056 -0.555 -0.4587 -0.7905 -0.9219 -0.3872 -1.4285 -1.1286 -1.2417 -1.4321 -1.754 -1.2841
Actin 0.0079 0.0057 0.0096 0.0119 0.0017 0.0049 0.004 0.0048 0.0023 0.0004 0.0024 0.0076 0.0301 0.003 0.0267 0.0053 0.0463 0.0106
Bud 0.0005 0.0018 0.0028 0.0012 0.0046 0.0014 0.0009 0.0006 0.0023 0.0008 0.0045 0.0007 0.0028 0.0088 0.0121 0.0017 0.0017 0.0028
Bud Neck 0.0009 0.0008 0.0046 0.0014 0.0029 0.0053 0.0009 0.0021 0.0048 0.0008 0.0004 0.0062 0.0027 0.0006 0.0054 0.0344 0.0012 0.0096
Bud Periphery 0.0011 0.0013 0.0036 0.0027 0.0025 0.0032 0.002 0.0013 0.0026 0.0013 0.0073 0.0012 0.0036 0.0035 0.0104 0.0039 0.0034 0.0065
Bud Site 0.003 0.0074 0.005 0.0038 0.0073 0.0004 0.003 0.0241 0.0092 0.0012 0.0061 0.0033 0.0087 0.0321 0.0132 0.0021 0.001 0.0012
Cell Periphery 0.0002 0.0005 0.0004 0.0002 0.0004 0.0001 0.0007 0.0006 0.0006 0.0002 0.0021 0.0002 0.0008 0.0011 0.0014 0.0008 0.0004 0.0004
Cytoplasm 0.0463 0.0501 0.0422 0.0382 0.0487 0.0552 0.0489 0.0786 0.0655 0.0663 0.0593 0.0909 0.0788 0.0903 0.0965 0.0965 0.0669 0.1445
Cytoplasmic Foci 0.1348 0.0984 0.1335 0.1198 0.1161 0.1475 0.1198 0.152 0.1157 0.1574 0.0838 0.1012 0.1987 0.1226 0.1521 0.1159 0.2455 0.1558
Eisosomes 0.0005 0.0005 0.0007 0.0003 0.0002 0.0003 0.0006 0.0004 0.0005 0.0003 0.0003 0.0002 0.0018 0.0005 0.0015 0.0009 0.0007 0.0004
Endoplasmic Reticulum 0.0015 0.0013 0.001 0.0009 0.0011 0.001 0.0016 0.0007 0.0011 0.0008 0.001 0.0009 0.0045 0.0014 0.0027 0.0024 0.0032 0.0024
Endosome 0.0193 0.0226 0.0178 0.0198 0.014 0.0192 0.0222 0.0276 0.0204 0.0098 0.004 0.011 0.0559 0.0477 0.0381 0.0149 0.094 0.0352
Golgi 0.006 0.0046 0.0054 0.0056 0.0051 0.005 0.0034 0.0064 0.0045 0.0025 0.002 0.0023 0.0133 0.0102 0.0116 0.0043 0.0295 0.0064
Lipid Particles 0.0144 0.0076 0.0174 0.0083 0.0082 0.0091 0.0126 0.0069 0.006 0.0057 0.0051 0.0069 0.0254 0.007 0.0091 0.0036 0.0126 0.0084
Mitochondria 0.0061 0.005 0.0098 0.0128 0.0061 0.0079 0.0145 0.02 0.0307 0.0051 0.0207 0.0118 0.0143 0.0098 0.0266 0.0235 0.0209 0.0112
None 0.7177 0.7525 0.6856 0.6615 0.7407 0.6909 0.7301 0.6443 0.6811 0.7035 0.7349 0.7151 0.4995 0.6239 0.5176 0.6498 0.382 0.5638
Nuclear Periphery 0.0014 0.0007 0.0016 0.0013 0.0012 0.0007 0.0007 0.0004 0.0009 0.0004 0.0006 0.0009 0.0051 0.0008 0.0014 0.0011 0.0019 0.0012
Nucleolus 0.0011 0.0011 0.0019 0.0015 0.0007 0.0007 0.0017 0.0011 0.0019 0.0013 0.0008 0.0006 0.0011 0.0024 0.0058 0.0012 0.0008 0.0006
Nucleus 0.0015 0.0018 0.0055 0.0022 0.0016 0.0017 0.0015 0.0018 0.0034 0.0012 0.0018 0.002 0.0021 0.0068 0.0105 0.002 0.0015 0.0026
Peroxisomes 0.0288 0.0247 0.0374 0.0917 0.0243 0.0341 0.0238 0.0225 0.0358 0.0377 0.0459 0.0276 0.0393 0.015 0.0457 0.0258 0.069 0.0273
Punctate Nuclear 0.0056 0.0099 0.0125 0.012 0.0105 0.01 0.0026 0.0021 0.008 0.002 0.0159 0.0079 0.0068 0.0092 0.0061 0.0075 0.0047 0.0056
Vacuole 0.0012 0.0017 0.0013 0.002 0.0017 0.0012 0.0037 0.0015 0.0023 0.0011 0.0009 0.0011 0.0036 0.0029 0.0043 0.0019 0.0097 0.0025
Vacuole Periphery 0.0004 0.0003 0.0004 0.0007 0.0005 0.0004 0.0007 0.0003 0.0005 0.0002 0.0002 0.0004 0.0011 0.0005 0.0012 0.0005 0.0031 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4219 8.3503 6.9708 12.116 17.4096 11.6698 10.8175 8.1915 13.0136 14.4639
Translational Efficiency 1.0515 0.7328 0.6455 0.5137 0.3421 0.6871 0.5466 0.9753 0.586 0.3738

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
555 1004 142 1444 1808 1774 183 1401 2363 2778 325 2845

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.32 650.63 848.33 755.58 633.83 690.21 886.59 814.84 645.69 675.91 869.87 784.76
Standard Deviation 71.03 98.87 84.01 105.06 68.93 77.20 89.25 105.10 72.65 87.75 89.05 109.17
Intensity Change Log 2 — -0.072834 0.309954 0.142913 — 0.122939 0.484171 0.362421 — 0.024623 0.396355 0.252636

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000351 0.001369 0.000723 0.001000 0.000342 0.001439 0.000296 0.000434 0.000344 0.001413 0.000482 0.000721
Bud Neck 0.035959 0.056107 0.002479 0.009376 0.037558 0.053472 0.002776 0.007291 0.037182 0.054424 0.002646 0.008350
Bud Site 0.004922 0.044896 0.007511 0.024807 0.006766 0.022618 0.002031 0.020031 0.006333 0.030670 0.004425 0.022455
Cell Periphery 0.000296 0.000347 0.000298 0.000207 0.000221 0.000235 0.000316 0.000191 0.000238 0.000275 0.000308 0.000199
Cytoplasm 0.218388* 0.143596* 0.303031* 0.449042* 0.260424* 0.163795* 0.354436* 0.481531* 0.250551* 0.156495* 0.331976* 0.465041*
Cytoplasmic Foci 0.427076* 0.304371* 0.096718 0.065995 0.388930* 0.376987* 0.101111 0.053777 0.397889* 0.350743* 0.099191 0.059978
Eisosomes 0.000494 0.000265 0.000160 0.000084 0.000416 0.000362 0.000149 0.000074 0.000435 0.000327 0.000154 0.000079
Endoplasmic Reticulum 0.000573 0.000778 0.003695 0.002534 0.001883 0.000682 0.004308 0.001882 0.001575 0.000717 0.004040 0.002213
Endosome 0.045376 0.093112 0.011357 0.007662 0.028489 0.050775 0.011492 0.006661 0.032455 0.066076 0.011433 0.007169
Golgi 0.012798 0.042008 0.000372 0.002616 0.016646 0.038225 0.000232 0.002475 0.015742 0.039592 0.000293 0.002546
Lipid Particles 0.034234 0.018238 0.008640 0.003347 0.035364 0.015776 0.008433 0.002255 0.035098 0.016666 0.008523 0.002809
Mitochondria 0.031479 0.107471* 0.001019 0.003322 0.039343 0.082308 0.001539 0.001829 0.037496 0.091402 0.001312 0.002587
Mitotic Spindle 0.000372 0.007534 0.000934 0.033217 0.005189 0.010071 0.000397 0.019337 0.004057 0.009154 0.000631 0.026382
None 0.001082 0.004049 0.004215 0.005006 0.002075 0.001183 0.004804 0.002322 0.001841 0.002219 0.004546 0.003684
Nuclear Periphery 0.000413 0.000503 0.001904 0.001715 0.000569 0.000246 0.001727 0.001237 0.000532 0.000339 0.001804 0.001479
Nuclear Periphery Foci 0.000293 0.000715 0.002489 0.001548 0.000269 0.000210 0.004463 0.001073 0.000275 0.000393 0.003600 0.001314
Nucleolus 0.001940 0.004916 0.002495 0.001010 0.001576 0.001238 0.002055 0.000417 0.001661 0.002567 0.002247 0.000718
Nucleus 0.086812 0.034435 0.460564* 0.240170* 0.101100 0.043194 0.394262* 0.280539* 0.097744 0.040029 0.423231* 0.260050*
Peroxisomes 0.056470 0.082939 0.003314 0.003030 0.032800 0.089937 0.003029 0.002892 0.038360 0.087408 0.003154 0.002962
Vacuole 0.037658 0.045234 0.087595 0.141525 0.037439 0.043993 0.101636 0.112675 0.037490 0.044441 0.095501 0.127318
Vacuole Periphery 0.003016 0.007117 0.000490 0.002786 0.002604 0.003254 0.000509 0.001079 0.002701 0.004650 0.000501 0.001945

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.92 -1.35 -2.03 1.39 -0.06 -3.48 -0.59 -0.59 3.34 -0.02 -4.51 -1.47 -2.16 3.14 -0.22
Bud Neck -4.09 9.69 8.03 13.07 -5.47 -4.89 16.29 13.84 17.69 -4.23 -6.33 18.97 15.99 21.98 -6.92
Bud Site -9.70 -0.52 -5.84 6.10 -2.36 -10.38 5.97 -4.00 3.60 -6.68 -13.49 1.11 -6.55 6.77 -5.32
Cell Periphery -0.89 -0.41 3.35 4.12 5.08 -0.30 -2.14 1.74 2.37 5.90 -0.97 -2.23 2.63 4.34 7.78
Cytoplasm 6.45 -2.49 -14.95 -25.44 -6.09 12.89 -3.86 -16.09 -27.51 -4.49 14.99 -3.90 -21.40 -37.42 -7.27
Cytoplasmic Foci 10.11 23.71 36.63 33.78 3.25 1.51 29.46 58.13 55.79 6.21 7.17 37.78 68.27 64.09 6.61
Eisosomes 4.84 6.75 9.33 9.57 5.79 1.16 5.92 8.19 12.22 7.16 2.93 7.66 10.63 15.02 9.09
Endoplasmic Reticulum -1.14 -6.80 -10.21 -9.59 3.34 3.60 -3.26 -2.39 -12.06 2.45 3.32 -5.84 -4.81 -14.86 3.89
Endosome -8.33 8.11 10.46 20.23 3.83 -8.69 6.73 15.27 20.99 2.66 -13.27 10.44 18.52 28.64 4.15
Golgi -9.32 9.78 8.15 13.98 -1.98 -9.42 15.75 12.81 17.52 -1.79 -12.73 18.12 15.19 22.46 -2.69
Lipid Particles 4.60 8.08 10.74 8.42 5.27 9.45 13.12 18.31 13.73 5.99 10.19 15.43 21.00 15.42 7.89
Mitochondria -10.19 7.68 7.30 16.70 -2.24 -9.26 15.82 16.75 20.22 -0.02 -13.53 17.96 17.98 26.09 -1.23
Mitotic Spindle -5.46 -1.12 -7.95 -5.08 -7.61 -2.63 4.32 -4.22 -2.25 -6.69 -3.75 3.86 -7.71 -5.10 -10.16
None -3.83 -2.91 -3.54 0.08 -0.46 2.50 -1.96 -1.07 -4.79 1.63 -1.13 -2.90 -2.93 -1.85 0.62
Nuclear Periphery -0.67 -9.42 -15.42 -11.45 -0.51 4.76 -8.10 -14.87 -20.99 -0.62 2.78 -11.99 -20.23 -21.97 -1.03
Nuclear Periphery Foci -2.37 -5.89 -8.31 -3.55 3.14 1.60 -3.10 -10.41 -11.30 2.25 -1.79 -4.67 -12.94 -9.52 3.06
Nucleolus -3.60 -0.67 2.68 5.10 3.52 1.29 -0.53 6.40 6.05 3.22 -2.97 -1.14 5.66 6.28 4.50
Nucleus 7.66 -15.06 -20.16 -32.85 7.91 13.90 -16.20 -26.03 -35.60 5.30 16.41 -21.83 -33.28 -48.17 9.44
Peroxisomes -4.16 11.11 11.50 18.58 0.54 -14.40 14.54 16.56 24.88 1.02 -14.98 18.42 20.09 31.05 1.09
Vacuole -1.98 -5.84 -24.85 -24.21 -5.65 -2.74 -8.27 -25.04 -23.53 -4.62 -3.46 -10.08 -36.06 -34.01 -7.30
Vacuole Periphery -5.05 4.34 1.78 7.04 -2.09 -2.47 10.46 9.51 10.37 -0.85 -6.33 11.36 4.79 9.44 -2.25
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene
Localization
Cell Percentages mitochondrion (20%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Put7

Put7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Put7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available