Standard name
Human Ortholog
Description Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.97 0.86 0.94 0.87 0.84 0.84 0.83 0.82 0.79 0.98 0.99 1.0 0.95 0.96 0.97 0.92 0.93 0.96 0.95 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.17 0 0.06 0.09 0.09 0.07 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 1 0 2 1 0 0 0 0 0 0 1 2 0 1 2 1
Bud 1 3 0 0 0 3 1 0 2 4 4 1 1 1 1 1 0 0 0 1 1 5 6 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 5 11 5 6 1 8 13 16 15 13 13 9 16 20 16 9 4 0 0 0 2 1 1
Cytoplasm 353 396 317 276 238 201 282 316 279 336 169 174 555 606 676 255 165 168 351 377 310 442 483 487
Endoplasmic Reticulum 1 4 2 0 3 2 18 14 8 20 4 8 5 5 3 12 3 10 2 2 1 4 6 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 1 2 1 0
Golgi 3 0 1 0 1 0 0 0 0 0 0 0 3 1 0 0 0 0 6 0 1 0 0 0
Mitochondria 3 1 0 0 0 7 3 6 15 10 16 19 0 0 0 3 1 0 2 1 1 2 1 0
Nucleus 0 4 4 3 0 0 3 7 4 15 3 5 1 0 0 7 4 2 0 2 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Vac/Vac Membrane 8 11 1 11 48 6 20 34 31 30 13 15 1 0 0 6 4 3 2 6 0 0 2 2
Unique Cell Count 368 411 319 285 276 213 326 378 333 407 205 221 564 612 679 268 172 174 380 404 322 467 514 516
Labelled Cell Count 372 424 336 295 296 220 337 391 356 430 225 236 575 629 700 300 186 187 380 404 322 467 514 516


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.3 14.7 14.0 13.8 14.9 9.7 12.2 11.9 10.9 11.1 10.5 10.3 15.1 15.8 16.7 21.5 21.7 19.8 16.1 20.3 24.3
Std Deviation (1e-4) 2.7 2.7 1.9 2.0 2.1 2.2 1.9 2.0 1.9 1.8 1.9 1.9 2.6 2.9 2.9 5.3 5.5 5.6 4.4 5.9 6.6
Intensity Change (Log2) – – – -0.01 0.09 -0.53 -0.2 -0.23 -0.35 -0.34 -0.41 -0.43 0.12 0.18 0.26 0.62 0.64 0.5 0.21 0.54 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -0.9 0 -0.7 0 0.9 0.2 1.5 1.3 -1.8 -0.7 -0.4 1.5 1.0 -0.7
Cytoplasm -2.3 -6.4 -3.5 -6.3 -7.2 -7.1 -7.5 -7.3 -8.2 -1.2 -0.6 0.4 -3.2 -2.7 -2.4
Endoplasmic Reticulum 0 0 0 3.6 2.7 0 3.3 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.8 0 5.1 5.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0.6 0 2.0 0 0 0 0 0 1.2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 7.6 0 4.2 5.2 5.3 4.7 4.2 4.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6772 10.5098 9.8755 9.0016 9.8071 9.6815 6.0255 9.492 7.7381 10.6034 7.1013 7.992 8.5829 11.6525 11.2171 11.4659 9.7385 10.8335
Actin 0.0575 0.001 0.0232 0.043 0.0778 0.0102 0.0427 0.0087 0.0488 0.0051 0.0053 0.0034 0.0611 0.0032 0.0158 0.0092 0.0331 0.0038
Bud 0.0014 0.0034 0.0007 0.0004 0.0002 0.0001 0.0021 0.0008 0.0008 0.0001 0.0018 0 0.0006 0.0037 0.0082 0.0006 0.0021 0.0009
Bud Neck 0.0074 0.0002 0.0013 0.0007 0.001 0.0015 0.0009 0.0009 0.001 0.0009 0.0007 0.0017 0.0038 0.0011 0.0006 0.0009 0.001 0.0016
Bud Periphery 0.0019 0.0007 0.0009 0.001 0.0002 0.0002 0.0021 0.0004 0.0015 0 0.0042 0.0002 0.0004 0.0016 0.003 0.0003 0.0075 0.0003
Bud Site 0.0029 0.0009 0.01 0.0004 0.0005 0.0001 0.0099 0.0126 0.0041 0.0002 0.0006 0.0001 0.0045 0.0162 0.0034 0.0013 0.0018 0.0001
Cell Periphery 0.0005 0.0001 0.0003 0.0001 0.0002 0.0001 0.0004 0.0002 0.0014 0.0001 0.0002 0.0005 0.0003 0.0002 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.7337 0.982 0.9203 0.8422 0.848 0.9448 0.6086 0.9579 0.8253 0.9865 0.7951 0.9087 0.7959 0.9602 0.9545 0.9131 0.8573 0.9583
Cytoplasmic Foci 0.0271 0.0016 0.0038 0.0091 0.0088 0.0028 0.0538 0.0047 0.0056 0.0017 0.0047 0.0093 0.039 0.0036 0.0021 0.0071 0.0102 0.0038
Eisosomes 0.0006 0 0.0002 0.0001 0.0005 0.0001 0.0004 0 0.0004 0 0.0001 0 0.0055 0 0.0001 0 0.0005 0
Endoplasmic Reticulum 0.0109 0.0014 0.0032 0.0033 0.0049 0.0021 0.0106 0.001 0.0387 0.0011 0.0087 0.0286 0.008 0.0007 0.0006 0.0131 0.0064 0.0008
Endosome 0.0453 0.0002 0.0022 0.0026 0.0121 0.0161 0.0894 0.0021 0.0103 0.0003 0.0845 0.0088 0.0201 0.0003 0.0005 0.0067 0.0291 0.0019
Golgi 0.0141 0.0001 0.0011 0.0019 0.0085 0.0029 0.046 0.0014 0.0033 0.0001 0.0291 0.0002 0.0064 0.0002 0.0013 0.011 0.0047 0.0024
Lipid Particles 0.0198 0 0.0004 0.0013 0.0068 0.0014 0.0529 0.0002 0.0018 0 0.0007 0.0012 0.0034 0.0001 0.0002 0.0025 0.002 0.0056
Mitochondria 0.0075 0.0001 0.0012 0.056 0.0012 0.0015 0.012 0.0013 0.0032 0 0.0481 0.0001 0.0015 0.0006 0.0013 0.0199 0.0078 0.0084
None 0.0165 0.0049 0.0045 0.0299 0.0144 0.0026 0.0205 0.0018 0.039 0.0015 0.0009 0.0126 0.0163 0.0039 0.0039 0.0038 0.003 0.0023
Nuclear Periphery 0.0098 0.0005 0.0113 0.0011 0.0025 0.0029 0.0062 0.0006 0.0036 0.0003 0.0031 0.0039 0.0054 0.0004 0.0005 0.0014 0.0177 0.0005
Nucleolus 0.0007 0 0.0001 0 0.0001 0.0001 0.0012 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0 0.0005 0
Nucleus 0.0175 0.0022 0.0109 0.0036 0.0066 0.0056 0.0019 0.0024 0.0017 0.0016 0.0019 0.0019 0.0083 0.0022 0.0027 0.0044 0.0044 0.0027
Peroxisomes 0.0106 0.0001 0.002 0.0016 0.0025 0.0003 0.0198 0.0019 0.0003 0.0001 0.0002 0.0003 0.0089 0.0006 0.0003 0.0029 0.0006 0.0053
Punctate Nuclear 0.0078 0.0005 0.0009 0.0011 0.0022 0.0004 0.0124 0.0007 0.0004 0.0002 0.0002 0.0024 0.0097 0.0008 0.0004 0.0005 0.0018 0.0008
Vacuole 0.0051 0.0002 0.0006 0.0003 0.0006 0.0023 0.0043 0.0002 0.0081 0.0001 0.0047 0.0156 0.0006 0.0002 0.0002 0.0007 0.005 0.0003
Vacuole Periphery 0.0017 0 0.0008 0.0001 0.0003 0.0019 0.0018 0.0001 0.0007 0 0.0051 0.0005 0.0003 0 0.0001 0.0002 0.003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 105.0861 69.6456 63.6987 67.9594 75.1717 94.163 54.0422 71.2917 76.4467 83.4182
Translational Efficiency 2.5904 2.5206 1.9762 2.1103 2.5591 3.245 2.7952 2.2411 2.1087 2.244

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1038 1227 1859 1469 1338 2498 1723 1646 2376 3725 3582 3115

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1016.09 1249.01 1518.72 1461.28 1013.07 1240.34 1386.25 1626.74 1014.39 1243.20 1455.00 1548.71
Standard Deviation 151.44 214.96 223.52 251.61 172.86 181.93 203.98 294.43 163.85 193.48 224.33 287.20
Intensity Change Log 2 — 0.297757 0.579828 0.524204 — 0.292002 0.452454 0.683250 — 0.294886 0.517641 0.605799

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000354 0.000276 0.000559 0.000492 0.000251 0.000148 0.000523 0.001029 0.000296 0.000190 0.000542 0.000776
Bud Neck 0.002055 0.000664 0.001223 0.004991 0.000626 0.001649 0.000637 0.006976 0.001250 0.001325 0.000941 0.006040
Bud Site 0.013688 0.006878 0.004732 0.014028 0.005904 0.006404 0.004645 0.025541 0.009305 0.006560 0.004690 0.020112
Cell Periphery 0.000298 0.000107 0.000154 0.000187 0.000113 0.000049 0.000095 0.000143 0.000194 0.000068 0.000126 0.000164
Cytoplasm 0.757210* 0.860915* 0.765242* 0.686363* 0.852774* 0.868719* 0.844533* 0.747963* 0.811025* 0.866149* 0.803382* 0.718913*
Cytoplasmic Foci 0.131319 0.028637 0.009431 0.019814 0.043864 0.028755 0.011233 0.017779 0.082071 0.028716 0.010298 0.018738
Eisosomes 0.000036 0.000007 0.000004 0.000017 0.000012 0.000007 0.000004 0.000010 0.000023 0.000007 0.000004 0.000013
Endoplasmic Reticulum 0.001316 0.000673 0.000203 0.001110 0.001320 0.000176 0.000112 0.000837 0.001319 0.000340 0.000159 0.000966
Endosome 0.003740 0.001504 0.000234 0.011058 0.001699 0.001216 0.000217 0.006120 0.002591 0.001311 0.000226 0.008449
Golgi 0.001716 0.001402 0.000149 0.006295 0.000411 0.000512 0.000148 0.007409 0.000981 0.000805 0.000148 0.006884
Lipid Particles 0.000947 0.000432 0.000534 0.001611 0.000440 0.000216 0.000372 0.001518 0.000662 0.000287 0.000456 0.001562
Mitochondria 0.000217 0.000298 0.000117 0.004680 0.000243 0.000496 0.000060 0.000552 0.000232 0.000431 0.000090 0.002499
Mitotic Spindle 0.002134 0.001859 0.002960 0.054972 0.000534 0.003958 0.003246 0.062546 0.001233 0.003267 0.003097 0.058974
None 0.002852 0.008019 0.007837 0.002751 0.007222 0.008483 0.009924 0.009603 0.005313 0.008331 0.008841 0.006372
Nuclear Periphery 0.000316 0.000089 0.000147 0.002123 0.000056 0.000065 0.000085 0.000270 0.000170 0.000073 0.000117 0.001144
Nuclear Periphery Foci 0.003822 0.004726 0.001959 0.008696 0.005334 0.001007 0.001904 0.002661 0.004673 0.002232 0.001933 0.005507
Nucleolus 0.000309 0.000132 0.000077 0.000833 0.000125 0.000180 0.000061 0.000227 0.000206 0.000164 0.000069 0.000513
Nucleus 0.032876 0.058745 0.186078* 0.058661 0.047542 0.051734 0.100980 0.062589 0.041135 0.054044 0.145145* 0.060737
Peroxisomes 0.000631 0.000590 0.000099 0.001927 0.000169 0.000711 0.000182 0.001219 0.000371 0.000671 0.000139 0.001553
Vacuole 0.042254 0.022727 0.018216 0.112676 0.031122 0.025265 0.021006 0.043854 0.035986 0.024429 0.019558 0.076310
Vacuole Periphery 0.001908 0.001319 0.000044 0.006714 0.000238 0.000249 0.000034 0.001152 0.000967 0.000601 0.000039 0.003775

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.64 -1.38 -1.37 -2.75 0.69 2.80 -1.31 -1.46 -2.37 0.47 2.54 -1.95 -2.03 -3.58 0.78
Bud Neck 6.10 -0.14 -1.80 -6.38 -1.66 -3.55 -3.02 -4.41 -2.27 -3.60 -0.31 -2.14 -4.84 -4.39 -3.71
Bud Site 4.81 6.23 2.34 -3.09 -4.62 0.07 -0.01 -4.21 -4.55 -4.94 3.86 4.49 -1.81 -5.81 -6.65
Cell Periphery 3.56 1.66 2.75 -3.37 3.69 7.84 -2.16 -0.77 -9.77 1.46 5.31 0.82 2.41 -9.92 4.14
Cytoplasm -11.60 6.65 13.27 26.09 8.64 -1.70 11.58 19.45 22.36 9.81 -9.99 13.69 23.09 34.72 12.24
Cytoplasmic Foci 21.15 26.28 25.84 8.78 -1.63 6.43 13.81 16.16 12.84 3.68 20.09 27.85 28.44 15.22 1.78
Eisosomes 10.03 10.04 7.73 -7.91 -8.63 6.23 5.81 4.06 -2.76 -2.15 11.58 11.33 8.46 -8.11 -7.53
Endoplasmic Reticulum 2.37 3.95 3.65 2.58 -1.25 2.01 2.04 1.82 -1.28 -1.56 2.90 3.22 2.94 0.31 -1.81
Endosome 5.50 10.87 2.75 -1.41 -3.99 1.96 7.41 1.33 -0.42 -3.65 5.40 12.96 2.81 -1.52 -5.32
Golgi 0.87 11.36 1.79 0.36 -2.32 -0.42 3.09 -1.66 -1.53 -2.23 1.28 10.57 -0.48 -1.13 -3.13
Lipid Particles 4.63 -2.22 -5.69 -10.33 -3.81 4.25 -5.42 -6.58 -8.80 -3.38 6.91 -5.38 -8.92 -14.03 -4.84
Mitochondria -0.33 1.57 -2.30 -1.92 -2.57 -0.73 1.48 0.51 1.00 -4.15 -0.81 1.76 -1.85 -0.47 -2.96
Mitotic Spindle 0.08 -1.99 -4.89 -5.09 -4.02 -3.87 -3.74 -7.41 -5.54 -5.19 -2.83 -4.30 -8.83 -7.25 -6.60
None -5.87 -8.02 0.34 6.83 13.61 -0.77 0.29 1.67 3.14 1.83 -3.70 -2.60 1.87 6.76 6.83
Nuclear Periphery 1.26 -0.12 -1.61 -3.55 -2.17 -2.47 -11.02 -14.11 -12.64 -7.27 1.14 -1.25 -3.21 -5.40 -3.19
Nuclear Periphery Foci -2.15 2.15 0.92 3.10 -0.83 10.93 7.29 7.92 -4.60 1.68 7.10 6.67 5.47 -0.58 0.06
Nucleolus 1.83 2.10 0.35 -2.85 -3.50 -0.60 1.60 0.16 1.33 -6.25 0.77 2.59 0.31 -0.81 -5.18
Nucleus -10.59 -39.56 -14.14 -4.00 28.25 -3.55 -21.79 -10.97 -8.86 10.78 -9.75 -42.94 -17.32 -9.60 28.36
Peroxisomes 0.36 7.45 1.66 0.60 -2.53 -3.21 0.73 -1.26 2.46 -1.68 -1.98 5.63 0.60 2.14 -3.10
Vacuole 3.80 -4.44 -29.19 -30.92 -26.60 0.12 -9.43 -22.78 -23.65 -15.69 2.43 -10.03 -36.47 -38.32 -29.90
Vacuole Periphery 0.81 2.68 1.10 0.11 -3.21 0.04 1.98 -0.10 -0.20 -3.35 1.16 2.98 0.96 -0.39 -3.83
Standard name
Human Ortholog
Description Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rdi1

Rdi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rdi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available