Standard name
Human Ortholog
Description Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.99 0.99 1.0 0.97 0.93 0.9 0.82 – 0.76 0.83 0.99 0.96 0.97 0.97 0.93 0.92 0.83 0.86 0.83 0.73 0.73 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.06 0.11 0.06 0.3 – 0.39 0.18 0 0 0 0 0 0 0.07 0.06 0.07 0.11 0.12 0.07
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 5 0 – 0 7 0 0 1 0 0 0 2 1 3 2 3 9
Bud 0 2 2 0 1 4 6 3 7 – 6 8 2 0 1 0 0 0 0 2 1 5 8 20
Bud Neck 0 0 6 1 5 1 1 3 3 – 0 1 0 0 0 0 0 0 0 1 0 1 2 7
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 2 2 6 7 8 – 5 8 1 3 6 15 5 11 0 0 2 2 5 5
Cytoplasm 90 198 316 314 264 395 508 426 283 – 244 297 459 411 475 216 153 155 91 184 253 173 259 339
Endoplasmic Reticulum 2 0 1 0 0 3 6 8 2 – 0 2 0 4 5 11 7 9 0 0 1 6 2 5
Endosome 0 0 0 0 0 1 0 0 1 – 0 1 0 3 1 1 1 0 1 2 2 0 1 2
Golgi 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 1 3 5 0 0 0 1 1 1
Mitochondria 6 5 2 3 0 25 59 28 103 – 125 65 0 0 3 6 3 1 7 13 20 25 41 30
Nucleus 1 0 0 0 1 2 4 4 4 – 2 3 0 0 1 1 1 1 0 0 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 – 0 0 1 1 1 0 2 1 0 0 0 0 1 1
SpindlePole 0 0 2 1 0 2 0 0 0 – 1 3 2 4 8 0 0 0 1 1 4 2 1 6
Vac/Vac Membrane 0 1 0 0 0 2 11 20 13 – 7 13 3 8 1 4 5 9 1 4 3 6 16 15
Unique Cell Count 95 204 320 318 265 408 548 471 346 319 358 465 429 489 222 164 169 110 215 305 236 355 459
Labelled Cell Count 99 208 331 319 273 438 602 505 425 390 408 469 434 505 255 180 194 110 215 305 236 355 459


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.0 4.7 4.4 5.1 4.7 5.0 5.6 4.6 – 4.4 5.0 4.8 6.7 8.9 6.9 7.3 7.1 4.0 3.9 4.4
Std Deviation (1e-4) 1.3 0.9 1.0 1.8 1.7 1.9 1.6 1.8 1.6 – 1.4 1.3 2.1 4.3 6.2 2.4 2.4 2.3 1.8 1.5 1.7
Intensity Change (Log2) – – – -0.08 0.14 0.01 0.1 0.27 -0.01 – -0.08 0.12 0.03 0.52 0.94 0.57 0.65 0.61 -0.23 -0.24 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9479 1.9075 1.0848 0.7688 1.3495 1.3663 -0.5616 -0.1647 -0.3142 -0.3352 -0.354 -0.4584 0.1396 0.6355 0.6666 1.1552 0.5422 0.5129
Actin 0.0159 0.0002 0.0049 0.0002 0.0247 0.0058 0.0473 0.0067 0.0077 0.0064 0.0019 0.0037 0.0093 0.0021 0.0055 0.0028 0.0031 0.0146
Bud 0.0001 0.0001 0 0 0.0013 0 0.0037 0.0014 0.0026 0.0055 0.0123 0.0018 0.0205 0.0311 0.0304 0.0367 0.0315 0.0124
Bud Neck 0.0023 0.0001 0.0005 0.0001 0.0004 0.0005 0.0019 0.0012 0.001 0.0021 0.0008 0.0006 0.0018 0.0018 0.0046 0.001 0.0047 0.0053
Bud Periphery 0.0002 0.0002 0.0001 0 0.0026 0.0001 0.0068 0.0033 0.0038 0.0125 0.0259 0.0012 0.0221 0.0229 0.0225 0.034 0.0253 0.0176
Bud Site 0.0014 0.0009 0.0007 0 0.0009 0.0003 0.0126 0.0304 0.0038 0.0051 0.0015 0.0003 0.0212 0.0949 0.0345 0.0042 0.0042 0.0012
Cell Periphery 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0.0007 0.0006 0.0007 0.0007 0.0003 0.0001 0.0016 0.0017 0.0017 0.001 0.0006 0.0006
Cytoplasm 0.0582 0.1398 0.1088 0.1522 0.2272 0.1851 0.1647 0.2352 0.2295 0.3352 0.2449 0.3201 0.0661 0.0814 0.0774 0.0769 0.0619 0.1468
Cytoplasmic Foci 0.0152 0.0053 0.0045 0.0021 0.0234 0.0135 0.0445 0.0105 0.0257 0.0268 0.0116 0.0187 0.0315 0.0111 0.0088 0.0075 0.0159 0.021
Eisosomes 0.001 0.0004 0.0003 0.0001 0.0004 0.0002 0.0005 0.0002 0.0002 0.0004 0.0001 0.0001 0.0004 0.0003 0.0004 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0.001 0.0006 0.001 0.0004 0.0034 0.0006 0.006 0.0012 0.0033 0.0028 0.0017 0.0008 0.0037 0.0012 0.0021 0.0019 0.0014 0.002
Endosome 0.0047 0.0019 0.0007 0.0002 0.0077 0.0016 0.017 0.0026 0.0222 0.0181 0.0566 0.0046 0.0117 0.0015 0.0011 0.0013 0.0079 0.0041
Golgi 0.002 0.0003 0.0002 0 0.0065 0.0008 0.0102 0.0004 0.0057 0.004 0.0234 0.0036 0.0024 0.0003 0.0005 0.0002 0.002 0.0016
Lipid Particles 0.0069 0.0002 0.0004 0.0001 0.017 0.0016 0.0201 0.0003 0.0052 0.0061 0.0013 0.0016 0.0088 0.0004 0.0016 0.0003 0.0323 0.0082
Mitochondria 0.0011 0.0016 0.0003 0.0001 0.0043 0.0002 0.0151 0.0006 0.0025 0.0343 0.0093 0.0007 0.0098 0.0019 0.0167 0.0043 0.006 0.0024
None 0.8539 0.846 0.8713 0.8436 0.5895 0.7783 0.6098 0.702 0.6709 0.5133 0.597 0.627 0.7194 0.6674 0.6493 0.669 0.7063 0.6692
Nuclear Periphery 0.0018 0.0002 0.0002 0.0001 0.0006 0.0001 0.0015 0.0003 0.0012 0.0016 0.0011 0.0005 0.0073 0.0034 0.0069 0.0086 0.0077 0.0054
Nucleolus 0.0033 0.0002 0.0001 0.0001 0.0002 0 0.0017 0.0002 0.0006 0.0063 0.0001 0.0002 0.0072 0.0041 0.0102 0.0084 0.0056 0.0028
Nucleus 0.0027 0.0004 0.0002 0.0003 0.0005 0.0004 0.0019 0.0008 0.0018 0.0046 0.002 0.0034 0.0202 0.0363 0.071 0.0684 0.0523 0.0389
Peroxisomes 0.0065 0.0002 0.0049 0 0.0867 0.0094 0.0266 0.0005 0.0047 0.0041 0.0004 0.0052 0.0031 0.0014 0.0014 0.0005 0.0089 0.0027
Punctate Nuclear 0.0204 0.0003 0.0003 0.0002 0.0015 0.001 0.0028 0.0006 0.0024 0.0024 0.0006 0.0047 0.025 0.0321 0.0492 0.0681 0.0177 0.041
Vacuole 0.0007 0.0008 0.0004 0.0001 0.0008 0.0003 0.0038 0.001 0.0039 0.0062 0.0046 0.0009 0.0056 0.0026 0.0035 0.0034 0.0031 0.0016
Vacuole Periphery 0.0002 0.0001 0 0 0.0003 0.0001 0.0008 0.0001 0.0006 0.0016 0.0025 0.0002 0.0012 0.0003 0.0007 0.0012 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0242 13.2273 7.0109 5.5832 14.2513 12.2533 12.5143 8.7687 5.3176 17.5987
Translational Efficiency 0.9796 1.1156 0.5631 0.767 1.0125 1.315 0.7633 0.611 0.7495 0.8185

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
181 984 586 1183 116 1619 151 147 297 2603 737 1330

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 675.57 727.62 956.09 877.64 739.07 696.29 929.82 909.91 700.37 708.13 950.71 881.21
Standard Deviation 801.54 90.41 110.70 199.68 66.88 486.67 118.44 145.13 627.88 388.12 112.83 194.66
Intensity Change Log 2 — 0.107080 0.501041 0.377524 — -0.086023 0.331240 0.300013 — 0.009423 0.414826 0.337549

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000499 0.001209 0.000719 0.000458 0.000059 0.000562 0.000170 0.000614 0.000327 0.000807 0.000606 0.000475
Bud Neck 0.001423 0.038611 0.004211 0.006192 0.005751 0.044131 0.001045 0.002227 0.003114 0.042044 0.003563 0.005754
Bud Site 0.000371 0.017242 0.012893 0.051110 0.001428 0.017810 0.001403 0.059605 0.000784 0.017595 0.010539 0.052049
Cell Periphery 0.000305 0.000432 0.000169 0.000288 0.000227 0.000190 0.000241 0.000142 0.000274 0.000281 0.000184 0.000272
Cytoplasm 0.503719* 0.322077* 0.223180* 0.541780* 0.434652* 0.358798* 0.259489* 0.468662* 0.476743* 0.344916* 0.230619* 0.533699*
Cytoplasmic Foci 0.156019 0.350601* 0.009885 0.032082 0.308220* 0.305688* 0.042307 0.067641 0.215464* 0.322666* 0.016527 0.036013
Eisosomes 0.000137 0.000241 0.000071 0.000041 0.000223 0.000220 0.000133 0.000070 0.000171 0.000228 0.000083 0.000044
Endoplasmic Reticulum 0.000192 0.000504 0.008746 0.001551 0.000371 0.000419 0.003370 0.001141 0.000262 0.000451 0.007645 0.001506
Endosome 0.002583 0.042224 0.003464 0.007432 0.019326 0.038532 0.006026 0.007337 0.009122 0.039928 0.003989 0.007421
Golgi 0.001210 0.011976 0.000121 0.005721 0.000970 0.011180 0.000086 0.000212 0.001116 0.011481 0.000113 0.005112
Lipid Particles 0.005891 0.010692 0.002432 0.001338 0.008117 0.010338 0.003692 0.002141 0.006761 0.010472 0.002691 0.001426
Mitochondria 0.000854 0.025732 0.000758 0.007990 0.000460 0.020116 0.000221 0.000133 0.000700 0.022239 0.000648 0.007122
Mitotic Spindle 0.000061 0.007811 0.001514 0.042432 0.000035 0.011364 0.000084 0.006947 0.000051 0.010021 0.001221 0.038510
None 0.003864 0.001591 0.004055 0.004382 0.001995 0.002855 0.002975 0.016924 0.003134 0.002377 0.003834 0.005768
Nuclear Periphery 0.000297 0.000250 0.002846 0.001093 0.000355 0.000301 0.001650 0.000719 0.000320 0.000281 0.002601 0.001052
Nuclear Periphery Foci 0.000143 0.000450 0.002601 0.001707 0.000620 0.000358 0.002045 0.003628 0.000329 0.000393 0.002487 0.001919
Nucleolus 0.000497 0.001751 0.000627 0.000351 0.000764 0.002016 0.000714 0.000440 0.000601 0.001916 0.000645 0.000361
Nucleus 0.299668* 0.061129 0.615714* 0.139555* 0.151297* 0.078965 0.555249* 0.101164 0.241718* 0.072223 0.603325* 0.135312*
Peroxisomes 0.000997 0.032589 0.001611 0.001303 0.003741 0.024074 0.000427 0.003730 0.002068 0.027293 0.001369 0.001571
Vacuole 0.021142 0.069531 0.104170 0.148068 0.060726 0.069397 0.118497 0.256371* 0.036602 0.069448 0.107106 0.160038
Vacuole Periphery 0.000129 0.003357 0.000213 0.005125 0.000663 0.002686 0.000177 0.000151 0.000338 0.002940 0.000206 0.004575

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 -0.79 0.08 1.29 2.73 -3.25 -6.34 -3.84 -0.38 -2.33 -1.29 -1.42 -0.51 1.31 2.15
Bud Neck -12.74 -8.20 -8.14 10.97 -1.90 -13.60 2.72 1.92 17.64 -3.70 -20.14 -1.59 -3.33 19.28 -2.79
Bud Site -10.01 -4.53 -8.91 -2.48 -4.60 -11.01 -1.74 -3.32 -1.36 -3.23 -15.03 -4.48 -9.39 -2.85 -5.73
Cell Periphery -0.19 0.81 0.89 1.15 0.45 1.28 0.17 5.33 2.90 4.36 0.21 0.88 1.09 1.27 0.83
Cytoplasm 8.99 16.64 3.41 -9.42 -22.68 3.39 6.90 2.27 -0.10 -3.97 8.79 18.39 3.06 -8.53 -22.09
Cytoplasmic Foci -14.31 13.35 11.88 44.31 -9.17 0.06 14.05 12.56 27.05 -2.21 -8.99 18.56 17.24 61.83 -6.86
Eisosomes -4.48 2.54 5.26 12.41 8.46 0.20 3.93 7.20 11.99 4.23 -3.60 5.07 9.13 20.02 9.94
Endoplasmic Reticulum -4.33 -20.00 -13.75 -11.56 16.72 -0.69 -7.79 -5.78 -5.71 4.48 -3.85 -20.82 -14.73 -13.58 17.00
Endosome -15.58 -2.80 -1.94 15.04 0.37 -3.88 2.87 2.44 13.79 -1.19 -12.84 2.44 2.47 22.85 0.27
Golgi -6.93 0.99 -0.39 7.43 -1.99 -8.64 2.86 2.13 9.49 -1.78 -9.75 1.47 -0.48 9.05 -2.02
Lipid Particles -1.96 1.26 1.87 11.09 6.47 -1.67 3.39 4.71 10.79 3.00 -2.38 2.40 3.44 16.77 7.60
Mitochondria -8.87 0.08 -1.49 7.80 -1.76 -11.12 2.08 2.80 11.31 2.76 -13.84 0.04 -1.67 11.00 -1.80
Mitotic Spindle -3.84 -3.22 -8.95 -5.86 -7.98 -5.87 -2.97 -4.23 2.21 -4.10 -7.01 -3.28 -9.13 -5.47 -8.27
None 1.91 0.57 -0.42 -7.19 -2.94 -1.01 -0.57 -1.55 -1.25 -1.40 0.82 0.08 -1.71 -4.00 -3.27
Nuclear Periphery 1.20 -24.04 -18.01 -18.81 9.75 0.98 -10.36 -6.15 -7.28 4.21 1.14 -25.12 -18.24 -19.36 9.59
Nuclear Periphery Foci -1.74 -10.28 -9.06 -5.80 3.94 1.71 -4.65 -4.30 -4.55 -2.13 -0.85 -10.81 -9.11 -8.49 2.89
Nucleolus -5.38 -1.12 -1.37 3.74 -0.84 -6.53 1.39 3.40 8.88 2.01 -9.03 0.15 -0.59 6.35 -0.68
Nucleus 13.38 -13.55 6.30 -18.50 30.40 5.55 -12.55 2.74 -1.50 14.12 13.35 -19.85 4.97 -17.29 32.61
Peroxisomes -13.70 0.45 0.26 13.82 -0.31 -12.05 3.62 0.31 11.89 -2.83 -19.33 2.47 1.95 20.80 -1.15
Vacuole -11.39 -16.63 -29.85 -22.98 -7.44 -0.62 -6.26 -13.00 -13.34 -7.17 -7.59 -15.98 -29.06 -27.08 -9.29
Vacuole Periphery -10.11 -4.46 -2.60 2.79 -2.42 -8.40 3.43 3.63 12.86 1.12 -14.38 2.05 -2.16 2.93 -2.42
Standard name
Human Ortholog
Description Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (31%), nucleus (1%), mixed (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rnr3

Rnr3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnr3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available