Standard name
Human Ortholog
Description RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0 0.1 0 0.06 0.06 0.05 0.05 – 0 0 0 0.07 0.08 0.07 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0.05 0 0.06 0.07 0 0.09 – 0.08 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.72 0.71 0.77 0.75 0.81 0.68 0.66 0.66 0.63 – 0.62 0.61 0.8 0.78 0.72 0.64 0.88 0.58 0.6 0.66 0.58 0.53 0.52 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.53 0.59 0.53 0.36 0.36 0.4 0.51 0.51 0.59 – 0.63 0.62 0.67 0.48 0.52 0.4 0.31 0.37 0.34 0.28 0.35 0.38 0.35 0.41
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0.07 0 0 0 0.06 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 2 0 – 0 2 0 0 1 0 0 0 2 0 1 0 1 5
Bud Neck 0 1 0 1 0 2 1 0 1 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 4 4 5 – 7 15 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 19 13 5 22 11 22 32 37 29 – 16 26 6 16 21 7 0 7 2 0 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Endosome 0 0 0 1 0 2 0 1 0 – 0 1 0 0 1 1 0 0 0 0 3 2 0 0
Golgi 1 1 1 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6 12 11 11 2 23 35 22 50 – 31 48 0 1 4 4 0 3 0 0 2 0 2 2
Nucleus 262 125 221 157 247 247 352 533 364 – 248 369 209 186 194 68 14 34 226 123 175 92 134 141
Nuclear Periphery 0 2 0 0 0 1 2 0 2 – 5 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 194 104 152 75 109 145 269 417 340 – 250 380 176 115 141 43 5 22 128 52 107 66 91 128
Peroxisomes 0 0 1 1 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 0 1 0 1 3
Vac/Vac Membrane 7 1 1 1 2 4 2 11 5 – 2 6 1 2 7 8 0 4 12 7 9 10 18 21
Unique Cell Count 366 177 286 210 306 363 532 812 578 398 609 262 240 270 107 16 59 377 187 304 175 257 309
Labelled Cell Count 489 259 394 269 372 448 699 1029 796 559 851 393 320 372 132 19 70 377 187 304 175 257 309


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.5 40.0 22.9 21.0 23.9 21.5 21.3 20.9 18.9 – 17.5 17.1 32.6 30.1 25.8 27.6 46.8 29.8 32.7 28.3 32.9
Std Deviation (1e-4) 17.8 17.4 6.7 7.5 6.8 7.0 6.5 6.2 5.9 – 5.7 5.5 9.1 9.6 9.4 11.8 12.4 13.9 11.4 10.7 11.9
Intensity Change (Log2) – – – -0.12 0.06 -0.09 -0.1 -0.13 -0.27 – -0.39 -0.42 0.51 0.4 0.18 0.27 1.03 0.38 0.52 0.31 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 4.2 1.4 2.7 2.8 2.1 2.3 – 1.7 1.9 0.5 2.9 3.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.7 -2.6 1.4 1.6 -1.0 2.6 – 2.1 2.3 -3.2 -2.6 -1.7 0 0 0
Nucleus -0.6 1.0 -2.6 -3.3 -3.6 -4.2 – -4.2 -4.9 0.7 0.1 -1.5 -2.7 1.0 -3.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -3.9 -4.3 -3.4 -0.7 -0.5 1.6 – 2.5 2.6 3.3 -1.2 -0.2 -2.3 -1.7 -2.2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.2082 44.0258 38.261 37.4065 36.7598 38.9349 26.4047 38.6643 34.8595 33.4076 31.6701 35.0102 25.2761 29.5336 30.7096 30.3242 26.44 30.7779
Actin 0 0 0 0.0001 0.0001 0 0.0048 0.0013 0.0057 0.0007 0.0036 0.0013 0.0001 0 0.0138 0 0.0001 0
Bud 0 0 0 0 0.0001 0.0001 0.0017 0.0001 0.0004 0.0003 0.0004 0.0002 0.0002 0 0.0002 0 0.0001 0
Bud Neck 0 0 0 0 0.0002 0.001 0.0003 0 0.0001 0.0001 0.0004 0.0002 0.0003 0.0002 0.0049 0 0.0001 0.0001
Bud Periphery 0 0 0 0.0001 0.0002 0.0001 0.001 0.0002 0.0004 0.0005 0.0006 0.0003 0.0001 0 0.0001 0 0.0002 0
Bud Site 0 0 0 0 0.0002 0.0001 0.0018 0.0001 0.0007 0.0004 0.0008 0.0001 0.0014 0.0001 0.0121 0 0.0002 0
Cell Periphery 0 0.0001 0 0.0001 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0003 0
Cytoplasm 0 0 0.0001 0.0001 0 0 0.004 0 0.002 0.0191 0.0006 0.001 0.0018 0 0.0004 0 0.0001 0
Cytoplasmic Foci 0 0 0.0016 0 0 0 0.0116 0.0001 0.0046 0.001 0.0008 0.0005 0.0205 0 0.0066 0 0.0088 0
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.001 0 0.0006 0 0.0004 0.0002 0.0004 0 0.0002 0 0 0
Endosome 0 0 0 0 0 0 0.0079 0.0001 0.0021 0.0006 0.0014 0.0011 0.0262 0 0.0026 0 0.0001 0
Golgi 0 0 0 0 0 0 0.0012 0.0001 0.0008 0.0004 0.0004 0.0003 0.0022 0 0.0028 0 0.0001 0
Lipid Particles 0 0 0.0008 0 0.0001 0 0.0033 0.0001 0.0023 0.0005 0.0004 0.0003 0.0154 0 0.0031 0 0.0035 0
Mitochondria 0.0012 0.0005 0.001 0.001 0.0128 0.0004 0.0047 0.0062 0.0034 0.0117 0.0049 0.0053 0.0015 0.0004 0.0007 0.0004 0.0069 0.0002
None 0 0 0.0003 0 0.0001 0 0.0029 0 0.0015 0.0002 0.0005 0.0001 0.0012 0 0.0003 0 0.0009 0
Nuclear Periphery 0 0.0002 0.0002 0.0011 0.0007 0.0001 0.0363 0.0004 0.0067 0.0012 0.015 0.0058 0.0024 0.0003 0.0003 0.0004 0.0008 0.0001
Nucleolus 0.191 0.1236 0.1203 0.0759 0.5121 0.302 0.1526 0.0824 0.078 0.0907 0.3888 0.2538 0.165 0.0906 0.0532 0.0411 0.4899 0.2847
Nucleus 0.8075 0.8748 0.8745 0.9208 0.4699 0.6956 0.6768 0.9068 0.8765 0.8691 0.5565 0.7239 0.7364 0.9078 0.8926 0.9576 0.4821 0.7146
Peroxisomes 0 0 0.0001 0 0.0001 0 0.0015 0.0001 0.0005 0.0003 0.0003 0.0001 0.0011 0 0.0046 0 0.0003 0
Punctate Nuclear 0 0.0001 0.0006 0.0001 0.0001 0.0002 0.0838 0.0017 0.0122 0.0015 0.0222 0.0036 0.021 0.0002 0.0007 0.0001 0.0023 0.0001
Vacuole 0 0.0002 0.0001 0.0002 0.0006 0.0003 0.0012 0.0001 0.0006 0.0008 0.0007 0.0005 0.0018 0.0001 0.0004 0.0001 0.0008 0
Vacuole Periphery 0.0001 0.0003 0.0002 0.0006 0.0021 0.0001 0.0015 0.0001 0.0008 0.0007 0.0011 0.001 0.0008 0.0001 0.0001 0.0001 0.0023 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 127.7547 123.4648 107.5494 138.2441 107.8312 93.0248 118.322 120.888 113.25 135.3627
Translational Efficiency 0.7599 0.7421 0.7822 0.555 0.6375 0.8399 0.7209 0.4721 0.5338 0.4794

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1911 92 236 963 1361 1690 240 97 3272 1782 476 1060

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2074.60 2736.43 3708.17 3325.48 1652.31 2275.69 3344.24 4095.31 1898.95 2299.48 3524.68 3395.93
Standard Deviation 524.61 677.83 574.42 826.07 538.74 556.82 549.53 927.84 569.90 572.85 590.73 864.87
Intensity Change Log 2 — 0.399462 0.837874 0.680729 — 0.461820 1.017194 1.309488 — 0.427441 0.920136 0.993593

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000430 0.000715 0.000933 0.000971 0.000507 0.000579 0.001046 0.000681 0.000462 0.000586 0.000990 0.000944
Bud Neck 0.002342 0.004913 0.006607 0.023749 0.001571 0.005702 0.013586 0.016630 0.002021 0.005661 0.010126 0.023097
Bud Site 0.000228 0.001132 0.001310 0.005036 0.000274 0.000782 0.001945 0.001683 0.000247 0.000800 0.001630 0.004729
Cell Periphery 0.000033 0.000026 0.000033 0.000291 0.000051 0.000055 0.000038 0.000079 0.000041 0.000054 0.000035 0.000271
Cytoplasm 0.000199 0.000295 0.000046 0.005651 0.001480 0.000150 0.000052 0.000506 0.000732 0.000157 0.000049 0.005180
Cytoplasmic Foci 0.000176 0.005494 0.003006 0.012231 0.000410 0.000739 0.001927 0.001765 0.000274 0.000984 0.002462 0.011274
Eisosomes 0.000168 0.000156 0.000299 0.000095 0.000109 0.000197 0.000273 0.000105 0.000144 0.000195 0.000286 0.000096
Endoplasmic Reticulum 0.000161 0.000163 0.000275 0.000737 0.000252 0.000277 0.000269 0.000211 0.000199 0.000271 0.000272 0.000689
Endosome 0.000004 0.000018 0.000008 0.006307 0.000012 0.000053 0.000010 0.000012 0.000008 0.000051 0.000009 0.005731
Golgi 0.000021 0.000153 0.000095 0.006150 0.000038 0.000083 0.000141 0.000114 0.000028 0.000087 0.000118 0.005597
Lipid Particles 0.000002 0.000027 0.000013 0.002567 0.000003 0.000035 0.000011 0.000009 0.000003 0.000034 0.000012 0.002333
Mitochondria 0.000744 0.001320 0.001945 0.007082 0.000953 0.001067 0.002600 0.002229 0.000831 0.001080 0.002275 0.006638
Mitotic Spindle 0.000017 0.000458 0.000060 0.028554 0.000109 0.005720 0.004265 0.000425 0.000055 0.005448 0.002180 0.025980
None 0.000769 0.000644 0.000546 0.004494 0.003075 0.000789 0.000718 0.001152 0.001728 0.000781 0.000633 0.004189
Nuclear Periphery 0.000015 0.000021 0.000033 0.000630 0.000020 0.000063 0.000057 0.000134 0.000017 0.000061 0.000045 0.000584
Nuclear Periphery Foci 0.000012 0.000080 0.000004 0.001100 0.000047 0.000329 0.000020 0.000007 0.000027 0.000316 0.000012 0.001000
Nucleolus 0.076796 0.193081 0.086624 0.032725 0.099865 0.149802 0.117682 0.036952 0.086392 0.152036 0.102283 0.033112
Nucleus 0.913743* 0.760239* 0.862231* 0.853786* 0.883763* 0.822624* 0.825072* 0.931302* 0.901273* 0.819403* 0.843495* 0.860879*
Peroxisomes 0.003776 0.030261 0.035284 0.003194 0.006962 0.010279 0.029583 0.005396 0.005102 0.011310 0.032409 0.003396
Vacuole 0.000146 0.000472 0.000442 0.002839 0.000236 0.000349 0.000412 0.000429 0.000184 0.000355 0.000427 0.002618
Vacuole Periphery 0.000218 0.000332 0.000207 0.001814 0.000261 0.000327 0.000293 0.000176 0.000236 0.000327 0.000251 0.001664

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.23 -13.59 -19.60 -6.21 -3.33 -2.92 -12.26 -4.94 -3.90 1.40 -5.27 -18.20 -19.63 -12.75 -1.72
Bud Neck -3.11 -11.68 -21.46 -16.44 -18.20 -15.53 -13.48 -7.44 -6.31 -3.78 -14.09 -15.79 -22.76 -20.04 -15.88
Bud Site -1.84 -11.47 -12.31 -6.60 -10.27 -5.97 -6.90 -4.95 -4.46 -2.73 -6.43 -9.83 -12.97 -11.79 -9.45
Cell Periphery -0.17 -0.56 -10.88 -10.11 -10.99 -0.40 0.04 -4.46 -4.20 -4.51 -1.03 -0.57 -11.36 -9.57 -11.41
Cytoplasm -0.80 1.23 -7.85 -7.27 -8.14 2.00 2.17 -0.82 -4.48 -4.75 1.93 2.41 -6.88 -8.18 -8.38
Cytoplasmic Foci -1.36 -6.87 -5.22 -0.07 -2.42 -1.69 -6.97 -3.92 -3.34 -1.33 -2.20 -9.34 -5.50 -4.67 -2.98
Eisosomes 0.55 -6.71 4.91 2.85 10.97 -5.22 -11.28 -0.71 3.98 9.15 -3.01 -11.67 4.24 5.20 15.32
Endoplasmic Reticulum -0.41 -8.36 -11.78 -11.32 -9.00 -0.65 -1.74 -2.72 -2.19 -2.04 -1.82 -5.51 -10.93 -7.13 -8.99
Endosome -1.42 -2.47 -2.51 -2.48 -2.50 -2.34 -0.70 -3.18 0.02 -3.07 -2.59 -2.22 -2.51 -2.43 -2.50
Golgi -1.65 -8.35 -2.99 -2.74 -2.85 -3.71 -9.39 -5.68 -4.63 -2.90 -4.53 -12.21 -3.03 -2.92 -2.82
Lipid Particles -1.55 -6.29 -1.93 -1.85 -1.90 -2.63 -6.23 -2.75 -1.33 -2.38 -2.76 -8.67 -1.95 -1.85 -1.92
Mitochondria -4.85 -11.14 -4.88 -4.16 -3.44 -2.52 -11.88 -7.08 -6.64 -1.71 -4.86 -15.92 -5.08 -4.74 -3.11
Mitotic Spindle -1.42 -3.07 -7.19 -6.98 -7.17 -3.97 -1.23 -1.64 -0.99 -1.25 -4.03 -1.26 -7.35 -5.05 -6.29
None 0.37 0.92 -7.64 -8.06 -8.30 3.55 3.59 0.00 -4.03 -4.11 2.86 3.56 -5.86 -7.95 -8.42
Nuclear Periphery -1.50 -6.09 -13.29 -13.16 -13.03 -3.37 -5.59 -4.27 -4.00 -3.99 -3.59 -7.38 -13.87 -13.09 -13.39
Nuclear Periphery Foci -0.91 1.13 -2.93 -2.25 -3.04 -2.32 1.47 -0.77 2.10 -1.77 -2.46 1.69 -2.89 -0.43 -3.04
Nucleolus -6.16 -1.87 9.11 8.07 6.46 -8.58 -2.24 7.22 12.43 7.03 -13.32 -3.23 13.22 21.96 10.24
Nucleus 6.59 5.49 9.46 -3.63 0.70 9.55 5.41 -0.12 -4.06 -3.66 14.84 7.97 7.95 -3.83 -1.40
Peroxisomes -3.75 -8.27 2.88 3.89 8.53 -4.14 -7.09 1.67 4.00 7.34 -7.91 -11.00 6.19 10.67 11.83
Vacuole -1.59 -7.26 -5.65 -4.91 -5.25 -1.70 -5.04 -3.90 -3.67 -3.42 -2.54 -8.55 -5.90 -5.64 -5.47
Vacuole Periphery -2.07 -0.44 -2.63 -1.62 -2.59 -1.46 -1.95 0.19 1.68 2.37 -2.45 -2.23 -2.55 -1.94 -2.31
Standard name
Human Ortholog
Description RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpb2

Rpb2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available