Standard name
Human Ortholog
Description Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.16 – 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 – 0.07 0.05 0.07 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 – – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0.12 0 – 0 0 0 0 0 0
Cytoplasm 0 0.14 0.15 0.17 0.19 0.26 0.17 – 0.14 0.14 0.14 0.15 0.07 0.21 0.22 0 0.21 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0
Endosome 0.07 0 0.2 0.11 0.32 0 0.18 – 0.05 0.05 0.09 0.06 0.2 0.2 0.32 0.24 0.05 – 0.12 0.1 0.07 0.11 0.1 0.1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.16 – 0 0 0 0 0.05 0.05
Mitochondria 0.86 0.8 0.38 0.48 0.32 0.7 0.52 – 0.78 0.74 0.76 0.77 0.65 0.45 0.24 0.12 0.16 – 0.68 0.69 0.71 0.65 0.61 0.6
Nucleus 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.16 0.17 0 0.07 – 0 0 0 0 0.16 0.16 0.26 0 0 – 0.06 0 0 0.09 0.09 0.05
SpindlePole 0.05 0 0.12 0.19 0.16 0.09 0.19 – 0.09 0.14 0.05 0.1 0.06 0.09 0 0.35 0.26 – 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.11 0.05 0.08 0.14 0.06 0.13 – 0.13 0.08 0.1 0.06 0.12 0.07 0.11 0.12 0.21 – 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 0 0 0 0 – 0 0 0 0 1 0 0 2 3 – 3 4 2 4 14 12
Bud 3 4 1 7 3 4 5 – 14 11 16 10 7 7 3 1 1 – 0 0 0 0 3 1
Bud Neck 0 0 0 0 0 0 1 – 0 1 0 2 0 2 0 0 0 – 3 0 0 1 5 7
Bud Site 2 0 0 0 0 2 1 – 3 3 4 2 1 1 0 1 0 – – – – – – –
Cell Periphery 3 6 1 6 3 0 2 – 3 0 3 2 8 3 5 2 0 – 1 0 0 0 1 1
Cytoplasm 5 25 6 19 27 33 34 – 28 30 32 34 16 46 59 0 4 – 0 4 2 0 3 2
Endoplasmic Reticulum 1 7 1 0 0 0 0 – 0 0 0 1 3 2 3 0 1 – 0 0 1 1 2 3
Endosome 15 4 8 12 47 3 36 – 11 10 20 15 43 44 86 4 1 – 31 20 6 25 42 30
Golgi 2 3 0 1 0 0 0 – 0 0 0 0 1 0 2 2 3 – 7 6 2 9 19 15
Mitochondria 196 141 15 52 47 88 103 – 158 158 168 179 142 99 64 2 3 – 180 142 67 152 250 179
Nucleus 1 1 0 0 0 4 9 – 6 1 8 6 0 2 0 1 1 – 0 1 0 1 0 1
Nuclear Periphery 2 0 0 1 0 0 1 – 1 1 1 3 0 0 0 0 0 – 6 2 0 3 4 1
Nucleolus 0 0 0 1 0 3 1 – 0 4 3 3 0 0 0 0 0 – 0 1 0 0 4 2
Peroxisomes 4 4 3 17 25 1 13 – 8 5 7 4 35 35 68 0 0 – 15 1 3 21 37 16
SpindlePole 12 2 5 21 23 11 38 – 19 30 10 23 12 20 10 6 5 – 6 2 3 2 8 5
Vac/Vac Membrane 22 19 2 9 20 7 25 – 26 17 22 14 27 15 29 2 4 – 3 13 2 4 9 16
Unique Cell Count 228 176 40 109 145 126 198 203 214 221 231 217 222 265 17 19 265 206 96 233 412 299
Labelled Cell Count 270 217 44 146 195 156 269 277 271 294 298 296 276 329 23 26 265 206 96 233 412 299


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.7 6.6 5.6 6.2 4.3 5.3 – 4.7 5.0 4.7 4.7 5.2 5.1 5.9 6.2 6.9 – 4.9 5.1 5.6
Std Deviation (1e-4) 0.7 1.0 2.6 2.2 2.1 2.5 2.2 – 2.4 2.5 2.0 2.5 1.1 1.5 2.0 1.3 1.4 – 1.2 1.4 2.5
Intensity Change (Log2) – – – -0.23 -0.08 -0.6 -0.32 – -0.47 -0.38 -0.49 -0.47 -0.35 -0.36 -0.17 -0.09 0.06 – -0.43 -0.37 -0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7883 -0.6285 -0.8615 -0.9639 -0.3961 -0.6709 0.9655 1.0263 1.1267 0.7641 1.153 0.8421 3.8145 3.7675 3.5819 3.7568 3.8775 3.6817
Actin 0.0424 0.0128 0.015 0.0063 0.0404 0.015 0.053 0.0178 0.0283 0.0435 0.0412 0.0095 0.0665 0.0126 0.0419 0.0653 0.0586 0.0302
Bud 0.0013 0.0065 0.0039 0.0008 0.0018 0.0007 0.0011 0.002 0.0014 0.0025 0.0017 0.0006 0.0011 0.0012 0.0016 0.003 0.0016 0.0008
Bud Neck 0.0023 0.0038 0.0017 0.0011 0.001 0.0043 0.0049 0.0012 0.0013 0.0015 0.0007 0.0034 0.0021 0.0013 0.0032 0.001 0.0007 0.0043
Bud Periphery 0.0022 0.0104 0.0035 0.0018 0.0027 0.0017 0.0021 0.004 0.0026 0.0061 0.0034 0.0012 0.0024 0.0024 0.0055 0.0144 0.0063 0.002
Bud Site 0.0101 0.0267 0.0154 0.0011 0.0045 0.0009 0.0118 0.026 0.0062 0.0017 0.0015 0.0013 0.0073 0.0071 0.0099 0.0032 0.0024 0.0018
Cell Periphery 0.001 0.0023 0.0009 0.0008 0.0003 0.0008 0.0024 0.0038 0.0008 0.0009 0.0006 0.0009 0.0013 0.0011 0.0032 0.001 0.0011 0.0012
Cytoplasm 0.0049 0.0446 0.0079 0.0102 0.0093 0.0136 0.026 0.0231 0.0204 0.0363 0.023 0.008 0.0142 0.0124 0.0161 0.0064 0.0071 0.0189
Cytoplasmic Foci 0.0885 0.0929 0.0656 0.0566 0.0798 0.0576 0.0801 0.0893 0.0654 0.0588 0.0499 0.0652 0.0725 0.112 0.0608 0.0468 0.0687 0.0555
Eisosomes 0.0024 0.0013 0.0009 0.0003 0.0003 0.0009 0.002 0.0006 0.001 0.0007 0.0007 0.0005 0.0024 0.0011 0.0018 0.0009 0.0011 0.0006
Endoplasmic Reticulum 0.0096 0.0071 0.0011 0.0027 0.0015 0.0028 0.0055 0.0018 0.0063 0.0031 0.0049 0.0022 0.0062 0.0046 0.0026 0.0004 0.0019 0.0011
Endosome 0.1143 0.1034 0.0964 0.1055 0.1812 0.0866 0.1862 0.1484 0.114 0.1281 0.1731 0.1299 0.1433 0.1416 0.0822 0.0408 0.1399 0.0754
Golgi 0.0926 0.0833 0.0539 0.0702 0.1206 0.0708 0.1118 0.1155 0.0844 0.078 0.0748 0.1301 0.0947 0.0992 0.0769 0.0499 0.0734 0.076
Lipid Particles 0.0631 0.038 0.0279 0.0359 0.0708 0.0344 0.0711 0.0119 0.0187 0.007 0.0461 0.0421 0.0361 0.0121 0.0143 0.0146 0.0226 0.0146
Mitochondria 0.4123 0.4294 0.6058 0.6527 0.4009 0.6298 0.3376 0.4614 0.5439 0.4848 0.4917 0.5066 0.4385 0.4777 0.5425 0.6796 0.4492 0.5973
None 0.0409 0.0611 0.0283 0.0068 0.0014 0.0153 0.0139 0.0311 0.0269 0.0462 0.0028 0.0083 0.0272 0.0281 0.0493 0.0159 0.0721 0.0295
Nuclear Periphery 0.0132 0.0023 0.0021 0.0051 0.0197 0.0008 0.0092 0.0007 0.0046 0.0006 0.0066 0.0015 0.0036 0.0021 0.0013 0.0002 0.0019 0.0002
Nucleolus 0.0027 0.0013 0.0022 0.0011 0.0009 0.0009 0.0012 0.0014 0.0005 0.0003 0.0003 0.0004 0.001 0.0004 0.0007 0.0004 0.0007 0.0003
Nucleus 0.0017 0.0015 0.001 0.0017 0.0016 0.0008 0.0035 0.0018 0.0009 0.0005 0.0017 0.0007 0.0014 0.0005 0.0007 0.0003 0.0007 0.0003
Peroxisomes 0.0737 0.0568 0.0565 0.0254 0.047 0.0515 0.0588 0.0427 0.0601 0.0899 0.0623 0.079 0.0609 0.0731 0.0723 0.0507 0.0822 0.0819
Punctate Nuclear 0.0074 0.0007 0.0017 0.0046 0.0027 0.0019 0.0049 0.0011 0.0019 0.0004 0.0009 0.0008 0.0078 0.0003 0.001 0.0005 0.0011 0.0003
Vacuole 0.0072 0.009 0.0052 0.0048 0.0056 0.0047 0.0085 0.0098 0.0059 0.0051 0.0073 0.0041 0.0057 0.0049 0.0092 0.0032 0.0038 0.0046
Vacuole Periphery 0.0063 0.0048 0.0032 0.0046 0.0058 0.0042 0.0046 0.0046 0.0046 0.004 0.0049 0.0037 0.0038 0.004 0.0029 0.0015 0.003 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2642 18.3512 12.4826 14.0628 19.962 10.2585 22.0339 17.2195 17.9769 17.7352
Translational Efficiency 1.2458 0.5648 0.7433 0.9336 0.394 1.1926 0.4547 0.8535 0.8415 0.5802

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
128 1117 277 1961 1265 2956 1879 92 1393 4073 2156 2053

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.41 736.81 937.15 856.29 637.69 790.27 825.84 977.10 642.07 775.61 840.14 861.70
Standard Deviation 97.52 103.84 99.27 123.88 74.15 96.92 108.22 109.49 77.82 101.70 113.40 125.78
Intensity Change Log 2 — 0.104325 0.451313 0.321132 — 0.309490 0.373007 0.615651 — 0.206854 0.414102 0.470592

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002249 0.003176 0.009703 0.010985 0.000969 0.001324 0.010807 0.019607 0.001087 0.001832 0.010665 0.011371
Bud Neck 0.014835 0.011866 0.016816 0.008827 0.016584 0.005110 0.012254 0.018096 0.016423 0.006963 0.012840 0.009242
Bud Site 0.010457 0.021684 0.053550 0.062881 0.008330 0.007814 0.073040 0.067625 0.008525 0.011618 0.070536 0.063094
Cell Periphery 0.001440 0.000451 0.000194 0.000206 0.000334 0.000144 0.000250 0.000231 0.000435 0.000228 0.000243 0.000207
Cytoplasm 0.016684 0.008699 0.023646 0.028475 0.012710 0.004505 0.028573 0.014165 0.013076 0.005655 0.027940 0.027834
Cytoplasmic Foci 0.065804 0.088419 0.184487* 0.137459 0.099094 0.065771 0.129334 0.162236 0.096035 0.071982 0.136420 0.138569
Eisosomes 0.000386 0.000162 0.000153 0.000127 0.000146 0.000092 0.000142 0.000194 0.000168 0.000111 0.000143 0.000130
Endoplasmic Reticulum 0.001650 0.000681 0.007693 0.001087 0.000755 0.000153 0.001658 0.001353 0.000837 0.000298 0.002433 0.001099
Endosome 0.028270 0.112060 0.099025 0.101074 0.099167 0.087613 0.115723 0.098183 0.092653 0.094318 0.113578 0.100945
Golgi 0.091907 0.189938* 0.073187 0.129704* 0.130251* 0.177267* 0.119613* 0.142540* 0.126728* 0.180742* 0.113648* 0.130279*
Lipid Particles 0.009967 0.003991 0.009341 0.001712 0.012344 0.002474 0.002204 0.006521 0.012126 0.002890 0.003121 0.001927
Mitochondria 0.613149* 0.435155* 0.037726 0.273682* 0.512238* 0.570721* 0.255742* 0.112940* 0.521510* 0.533543* 0.227731* 0.266479*
Mitotic Spindle 0.001530 0.013846 0.200707* 0.081089 0.009839 0.014597 0.068021 0.105279* 0.009075 0.014391 0.085068 0.082173
None 0.000447 0.000366 0.000373 0.000308 0.000899 0.000228 0.000253 0.000180 0.000857 0.000266 0.000268 0.000302
Nuclear Periphery 0.001028 0.000101 0.002125 0.000202 0.000713 0.000064 0.000221 0.000855 0.000742 0.000074 0.000466 0.000232
Nuclear Periphery Foci 0.000791 0.000209 0.004030 0.000417 0.000323 0.000113 0.000677 0.001292 0.000366 0.000139 0.001107 0.000456
Nucleolus 0.000492 0.000719 0.003245 0.000415 0.002280 0.000378 0.000666 0.004066 0.002116 0.000472 0.000997 0.000578
Nucleus 0.010705 0.001385 0.026655 0.001787 0.005324 0.000606 0.002819 0.002347 0.005818 0.000819 0.005881 0.001812
Peroxisomes 0.108361* 0.083067 0.147929* 0.082241 0.059102 0.041642 0.095404 0.188429* 0.063628 0.053003 0.102153* 0.087000
Vacuole 0.009608 0.013164 0.086976 0.062638 0.014754 0.011669 0.065310 0.044266 0.014281 0.012079 0.068093 0.061814
Vacuole Periphery 0.010239 0.010863 0.012443 0.014685 0.013845 0.007714 0.017289 0.009594 0.013514 0.008577 0.016667 0.014457

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.16 -5.90 -7.50 -6.79 -0.76 -1.00 -8.88 -3.20 -3.14 -1.42 -2.16 -9.77 -9.90 -9.33 -0.44
Bud Neck 0.48 -0.45 0.96 1.51 2.97 5.93 1.97 -0.41 -1.89 -0.93 5.06 1.64 3.56 -2.70 3.18
Bud Site -3.52 -6.62 -14.73 -11.79 -1.32 0.54 -20.87 -4.42 -4.46 0.11 -2.90 -21.90 -19.68 -18.52 1.87
Cell Periphery 2.22 2.82 2.82 3.24 -0.09 3.67 1.85 1.84 -1.80 0.54 3.24 3.30 3.98 0.76 2.31
Cytoplasm 1.87 -1.47 -2.49 -10.86 -0.87 5.43 -7.47 -0.86 -3.80 4.17 5.17 -7.60 -7.53 -14.69 0.10
Cytoplasmic Foci -2.58 -8.96 -7.89 -10.50 4.13 8.50 -6.18 -3.76 -6.12 -1.85 6.63 -8.62 -8.76 -18.33 -0.54
Eisosomes 2.30 2.29 2.68 2.63 2.86 4.89 0.24 -1.83 -4.11 -2.01 4.15 1.55 2.81 -2.81 1.90
Endoplasmic Reticulum 1.47 -4.53 0.82 -1.95 5.59 3.55 -4.48 -1.93 -3.29 0.10 3.09 -6.49 -1.74 -7.09 5.86
Endosome -13.16 -7.48 -13.28 2.27 -0.64 2.29 -2.82 0.29 -0.59 1.48 -0.40 -3.85 -1.46 -1.49 2.90
Golgi -5.80 1.40 -2.18 8.80 -7.49 -7.59 2.08 -0.24 2.19 -0.89 -9.51 2.72 -0.15 10.87 -3.35
Lipid Particles 1.36 -0.02 1.86 3.05 5.63 6.74 6.89 1.84 -1.53 -1.58 6.70 6.36 7.37 1.76 2.58
Mitochondria 5.41 18.32 10.81 13.36 -25.98* -5.13 21.61 16.49 19.93 6.17 -1.23 26.26 22.27 31.38 -4.09
Mitotic Spindle -7.62 -12.36 -20.35 -16.17 7.08 -3.03 -15.67 -4.09 -3.88 -1.53 -3.84 -19.15 -18.31 -17.21 0.28
None 0.65 0.08 0.94 0.51 1.01 4.09 3.99 3.93 0.34 0.66 3.98 3.93 3.36 -0.41 -0.27
Nuclear Periphery 1.06 -0.86 0.92 -3.28 1.90 1.31 0.96 -0.19 -1.17 -0.92 1.46 0.54 1.07 -4.20 1.60
Nuclear Periphery Foci 0.84 -2.57 0.53 -2.93 3.75 3.39 -2.57 -1.83 -2.15 -1.08 2.47 -3.92 -1.12 -4.55 3.39
Nucleolus -0.32 -4.09 0.29 1.22 5.11 3.06 2.48 -0.64 -1.35 -1.22 2.96 1.86 2.72 -0.64 2.03
Nucleus 1.78 -2.53 1.66 -1.27 5.58 4.26 1.66 0.77 -2.47 -0.44 4.47 -0.76 3.22 -3.86 5.61
Peroxisomes 1.28 -1.62 1.37 0.20 4.80 4.16 -6.68 -4.81 -5.52 -3.39 2.50 -7.07 -4.22 -7.60 2.86
Vacuole -0.63 -8.68 -11.11 -16.70 3.10 2.00 -16.50 -2.73 -2.92 0.45 1.56 -18.54 -16.68 -18.62 1.25
Vacuole Periphery -0.23 -0.40 -1.53 -3.04 -1.12 5.35 -2.24 0.85 -0.27 1.45 4.57 -2.13 -0.58 -6.46 1.78
Standard name
Human Ortholog
Description Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Rrg9

Rrg9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrg9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available