Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.16 0.23 0.21 0.06 0.05 0.06 0 0 0
Golgi 0 0.1 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.17 0.11 0 0.13 0.05 0.08 0.05 0.09 0.11 0.14
Mitochondria 0.86 0.85 0.95 0.95 0.88 0.91 0.93 0.9 0.94 0.94 0.95 0.96 0.9 0.91 0.78 0.14 0.06 0.15 0.75 0.74 0.75 0.69 0.66 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.06 0.06 0.16 0.15 0.07 0.14 0.11 0.13 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0.06 0.08 0.09 0.08 0.11 0.12 0.07 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.14 0.19 0.14 0 0 0.07 0.09 0.09 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.21 0.08 0 0 0 0 0.05 0.05 0 0 0 0.1 0.06 0.1 0.29 0.28 0.2 0.05 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 1 4 0 2 0 0 0 0 0 0 0 2 4 0 0 1 0 11 5 0 3 15 15
Bud 12 2 3 14 7 2 5 13 10 5 13 14 0 2 10 2 4 5 3 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 3 0 1 1 3 7 1 3 0 4 0 0 0 0 1 0 – – – – – –
Cell Periphery 18 13 4 3 3 1 1 2 3 4 2 4 9 11 10 5 5 7 1 0 0 0 2 1
Cytoplasm 6 4 2 7 16 10 7 18 5 4 6 4 3 6 9 6 3 8 0 1 0 0 0 0
Endoplasmic Reticulum 7 4 4 2 2 3 4 1 0 1 5 3 2 5 4 2 1 5 2 0 0 0 0 2
Endosome 20 9 0 0 12 2 2 11 2 2 4 0 15 15 28 9 11 15 36 14 10 8 15 17
Golgi 16 30 0 0 3 0 0 0 2 1 0 0 29 40 70 6 2 9 35 26 7 18 44 53
Mitochondria 517 253 169 239 264 265 386 482 208 327 294 433 320 372 321 8 3 11 490 234 128 144 263 245
Nucleus 1 1 1 0 0 3 3 2 4 1 0 2 0 1 1 4 2 2 1 0 0 0 0 0
Nuclear Periphery 11 8 7 14 18 46 62 36 30 39 40 44 10 11 5 2 0 2 4 3 1 3 4 2
Nucleolus 19 6 2 12 18 23 37 40 25 41 22 28 16 7 8 0 1 1 2 0 0 0 2 4
Peroxisomes 14 2 1 7 9 2 1 12 1 5 2 3 27 15 14 8 9 10 27 8 12 19 35 30
SpindlePole 3 3 3 2 2 3 6 14 1 5 2 4 4 4 5 6 5 5 4 1 0 3 0 0
Vac/Vac Membrane 110 63 14 9 13 10 9 24 10 7 6 3 37 25 39 16 13 14 31 14 5 5 11 9
Unique Cell Count 603 297 177 252 300 290 415 533 222 347 311 452 355 407 409 56 47 71 656 317 171 209 401 388
Labelled Cell Count 762 399 217 309 370 371 526 662 302 445 397 546 474 518 524 74 61 94 656 317 171 209 401 388


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.2 5.2 6.3 7.2 6.1 5.7 6.3 5.6 6.1 5.7 5.8 8.2 8.0 7.7 8.8 8.1 8.2 8.1 9.1 9.0
Std Deviation (1e-4) 1.4 1.5 1.4 1.8 1.9 1.6 1.7 1.7 1.4 1.6 1.7 1.5 1.4 1.4 1.3 2.2 2.1 2.1 1.6 1.7 1.7
Intensity Change (Log2) – – – 0.29 0.48 0.24 0.14 0.28 0.12 0.23 0.15 0.17 0.67 0.63 0.58 0.76 0.65 0.67 0.65 0.81 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2.0 0 0 0 0 1.6 0 1.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.9 4.3 5.9 0 0 0
Mitochondria -0.3 -2.7 -1.7 -1.1 -2.1 -0.8 -0.6 -0.5 0.2 -2.1 -1.7 -5.1 -12.4 -12.9 -12.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.8 1.0 3.9 3.8 1.4 3.3 2.8 3.2 2.4 -0.7 -0.8 0 0 0 0
Nucleolus 2.1 2.6 3.2 3.5 3.1 4.0 4.2 2.9 2.7 2.0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 -1.6 -2.1 -3.3 -1.7 -1.4 -3.3 -3.2 0 0.9 -0.8 0.6 4.0 3.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5985 4.3828 4.1726 4.0148 4.0427 4.2116 2.8772 3.1256 3.1013 2.772 3.0719 2.8521 4.6099 4.8996 4.4686 4.1514 4.0319 4.465
Actin 0.0158 0.0055 0.0073 0.0011 0.0008 0.0036 0.056 0.0104 0.018 0.0103 0.0055 0.0133 0.0423 0.025 0.0181 0.0437 0.01 0.002
Bud 0.0002 0.0024 0.0015 0.0001 0.0001 0.0002 0.0007 0.0038 0.0008 0.0009 0.0003 0.0008 0.0015 0.0011 0.0006 0.0002 0.0003 0.0001
Bud Neck 0.0005 0.0002 0.0004 0 0 0.006 0.0018 0.0003 0.001 0.0025 0 0.0006 0.0012 0.0003 0.0004 0.0001 0.0001 0.0001
Bud Periphery 0.0005 0.0042 0.004 0.0002 0.0001 0.0008 0.0013 0.0082 0.0017 0.0013 0.0008 0.0007 0.0037 0.0019 0.0012 0.0005 0.0009 0.0002
Bud Site 0.0005 0.0041 0.002 0.0001 0.0002 0.001 0.0082 0.0022 0.0026 0.0036 0.0002 0.0014 0.0046 0.0028 0.0029 0.0004 0.0002 0.0001
Cell Periphery 0.0002 0.0003 0.0003 0.0001 0 0.0005 0.0016 0.0003 0.0004 0.0002 0.0001 0.0001 0.0007 0.0005 0.0006 0.0004 0.0001 0.0001
Cytoplasm 0.005 0.0005 0.0014 0 0.0001 0.0011 0.0027 0.0008 0.001 0.0013 0 0.0037 0.0037 0.0005 0.0008 0.0001 0.0001 0.0007
Cytoplasmic Foci 0.0117 0.0103 0.0111 0.0032 0.0076 0.0077 0.0189 0.0148 0.004 0.0432 0.0008 0.0133 0.0165 0.0113 0.0179 0.0061 0.005 0.009
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0001 0.0002 0.0009 0.0002 0.0011 0.0002 0.0003 0.0002 0.001 0.0008 0.0006 0.0011 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0003 0.0014 0 0.0001 0.0004 0.0014 0.0004 0.0007 0.0004 0.0001 0.0003 0.0017 0.0001 0.0005 0.0001 0.0001 0.0003
Endosome 0.0366 0.0301 0.0258 0.0036 0.007 0.0106 0.0339 0.0403 0.0202 0.023 0.003 0.0382 0.0327 0.0167 0.0281 0.0036 0.0048 0.0265
Golgi 0.0252 0.0406 0.0351 0.0103 0.0269 0.0248 0.0429 0.0956 0.0311 0.0442 0.0063 0.0499 0.0738 0.0337 0.0209 0.0126 0.0055 0.04
Lipid Particles 0.0438 0.0096 0.0166 0.0068 0.0089 0.0106 0.0558 0.0119 0.0038 0.0023 0.0022 0.0025 0.0434 0.0163 0.0106 0.0025 0.0012 0.0218
Mitochondria 0.8086 0.8316 0.8517 0.9381 0.8948 0.9101 0.7178 0.7766 0.8913 0.8463 0.9601 0.83 0.6899 0.835 0.8722 0.8846 0.8614 0.8808
None 0.0007 0.0003 0.0007 0 0.0001 0.0025 0.0013 0.0121 0.0127 0.0001 0.0001 0.0001 0.0149 0.0003 0.0003 0.0003 0.0001 0.0003
Nuclear Periphery 0.0048 0.0003 0.0062 0 0.0013 0.0008 0.0102 0.0002 0.0004 0.0001 0 0.0001 0.0051 0 0.0002 0 0.0001 0.0018
Nucleolus 0.0004 0.0003 0.0003 0 0.0003 0.0004 0.0027 0.0003 0.0001 0.0001 0 0 0.003 0.0002 0.0013 0 0 0.0001
Nucleus 0.001 0.0003 0.002 0 0.0002 0.0003 0.0055 0.0002 0.0002 0.0001 0 0.0001 0.0024 0.0001 0.0004 0 0.0001 0.0002
Peroxisomes 0.0275 0.0503 0.0227 0.0344 0.0352 0.0099 0.0243 0.0142 0.0046 0.0169 0.0187 0.0412 0.0331 0.0502 0.0125 0.043 0.1075 0.0106
Punctate Nuclear 0.0015 0.0002 0.0004 0 0.0152 0.0014 0.0034 0.0001 0.0005 0.0002 0 0.0001 0.0135 0.0001 0.0004 0 0 0.0016
Vacuole 0.0042 0.0028 0.003 0.0005 0.0003 0.0024 0.0049 0.003 0.0011 0.0013 0.0004 0.0016 0.0063 0.0015 0.007 0.0001 0.0008 0.001
Vacuole Periphery 0.0089 0.0058 0.006 0.0011 0.0008 0.0046 0.004 0.0041 0.0026 0.0013 0.0011 0.0019 0.0049 0.0018 0.0022 0.0003 0.0018 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.5082 29.6209 15.386 36.3183 32.221 56.2988 41.1808 42.4494 52.2302 43.8647
Translational Efficiency 2.7414 2.3034 4.0285 2.2823 2.3582 1.5934 2.2281 2.4905 1.8989 2.0257

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
181 1450 233 1131 1307 1993 1346 1508 1488 3443 1579 2639

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.56 856.03 1064.75 1051.92 807.41 890.60 1060.99 1089.41 793.80 876.04 1061.54 1073.34
Standard Deviation 142.04 159.85 160.22 163.32 114.66 137.15 167.53 170.64 123.85 148.13 166.48 168.57
Intensity Change Log 2 — 0.299486 0.614268 0.596778 — 0.141476 0.394038 0.432174 — 0.216758 0.500149 0.510691

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005304 0.002978 0.014513 0.005626 0.001569 0.001799 0.002183 0.009981 0.002023 0.002296 0.004002 0.008115
Bud Neck 0.005066 0.003081 0.011656 0.002981 0.001810 0.003901 0.013072 0.001316 0.002206 0.003555 0.012863 0.002030
Bud Site 0.009899 0.006650 0.049311 0.020084 0.003839 0.006885 0.028040 0.010288 0.004576 0.006786 0.031179 0.014486
Cell Periphery 0.001194 0.000537 0.000560 0.000326 0.000318 0.000490 0.000463 0.000506 0.000424 0.000510 0.000478 0.000429
Cytoplasm 0.002348 0.001828 0.012664 0.003992 0.000969 0.002823 0.006282 0.002247 0.001137 0.002404 0.007224 0.002995
Cytoplasmic Foci 0.029682 0.022147 0.070643 0.040274 0.021980 0.023154 0.018442 0.018521 0.022917 0.022730 0.026145 0.027844
Eisosomes 0.000124 0.000077 0.000145 0.000079 0.000051 0.000061 0.000076 0.000108 0.000060 0.000068 0.000086 0.000096
Endoplasmic Reticulum 0.000534 0.000385 0.003676 0.001341 0.000198 0.000278 0.000635 0.000458 0.000239 0.000323 0.001084 0.000836
Endosome 0.070204 0.047580 0.076867 0.046604 0.050469 0.052408 0.050174 0.023874 0.052870 0.050375 0.054113 0.033615
Golgi 0.046166 0.036255 0.067670 0.079372 0.031923 0.034182 0.020654 0.041618 0.033655 0.035055 0.027591 0.057798
Lipid Particles 0.019189 0.005896 0.004986 0.003872 0.005641 0.005799 0.002403 0.000616 0.007289 0.005840 0.002784 0.002011
Mitochondria 0.698931* 0.803199* 0.423980* 0.681190* 0.825258* 0.802617* 0.702587* 0.833826* 0.809892* 0.802862* 0.661475* 0.768411*
Mitotic Spindle 0.010325 0.010271 0.114290* 0.046910 0.004213 0.009011 0.067500 0.010496 0.004957 0.009542 0.074405 0.026102
None 0.008293 0.000722 0.000418 0.000387 0.000140 0.000880 0.000689 0.000232 0.001131 0.000813 0.000649 0.000299
Nuclear Periphery 0.000130 0.000079 0.001499 0.001754 0.000083 0.000264 0.001361 0.000145 0.000089 0.000186 0.001381 0.000835
Nuclear Periphery Foci 0.001061 0.000284 0.002073 0.001833 0.000198 0.000413 0.001241 0.000519 0.000303 0.000359 0.001364 0.001082
Nucleolus 0.001967 0.000415 0.002384 0.000214 0.000123 0.000655 0.002053 0.000164 0.000347 0.000554 0.002102 0.000185
Nucleus 0.001258 0.000538 0.012642 0.001164 0.000114 0.001065 0.004367 0.000432 0.000253 0.000843 0.005588 0.000746
Peroxisomes 0.074476 0.046624 0.066181 0.014341 0.037488 0.035843 0.027689 0.035033 0.041987 0.040383 0.033369 0.026165
Vacuole 0.007096 0.006456 0.043484 0.024826 0.005918 0.010126 0.031938 0.004886 0.006061 0.008580 0.033642 0.013432
Vacuole Periphery 0.006754 0.003997 0.020358 0.022831 0.007701 0.007346 0.018150 0.004734 0.007586 0.005936 0.018476 0.012490

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.09 -2.62 -0.16 -2.91 2.97 -0.48 -1.08 -6.34 -6.35 -5.83 -0.56 -2.80 -6.65 -6.97 -4.11
Bud Neck 1.43 -3.22 1.53 0.02 4.89 -2.60 -7.41 0.87 4.03 8.14 -2.13 -8.02 0.21 3.19 8.57
Bud Site 0.62 -4.37 -2.02 -5.70 3.62 -2.47 -9.46 -4.45 -2.25 6.60 -1.88 -10.50 -6.80 -5.83 6.24
Cell Periphery 1.96 1.82 2.70 2.04 1.62 -1.30 -1.61 -1.50 -0.10 -0.37 -0.79 -0.59 -0.05 0.85 0.66
Cytoplasm 0.33 -2.27 -1.60 -2.53 1.72 -3.04 -5.22 -3.06 0.85 3.98 -2.79 -5.58 -3.91 -1.56 3.43
Cytoplasmic Foci 1.31 -4.42 -1.67 -6.00 3.92 -0.54 1.38 1.46 2.28 0.07 0.04 -1.37 -2.17 -2.83 -0.68
Eisosomes 2.31 -0.83 2.20 -0.35 3.39 -2.17 -4.02 -6.69 -5.72 -3.33 -1.67 -4.06 -5.69 -5.16 -1.17
Endoplasmic Reticulum 1.02 -4.65 -3.74 -4.64 3.37 -2.19 -5.93 -2.60 -1.75 1.69 -2.08 -6.97 -5.83 -4.87 1.72
Endosome 2.04 -0.49 2.16 0.28 3.87 -0.49 0.02 7.74 9.36 7.74 0.70 -0.33 5.86 6.95 6.57
Golgi 1.23 -1.92 -3.63 -8.26 -1.44 -0.77 4.14 -2.89 -2.41 -7.20 -0.53 2.24 -7.25 -7.90 -10.03
Lipid Particles 2.23 2.32 2.58 1.47 0.94 -0.14 2.68 4.36 6.78 5.96 1.04 3.46 4.09 5.25 1.83
Mitochondria -3.70 7.62 0.74 9.41 -9.48 2.22 10.09 -0.75 -3.15 -11.09 0.80 12.50 4.34 4.43 -9.60
Mitotic Spindle 0.01 -7.50 -6.97 -8.92 4.82 -4.10 -14.27 -4.88 -1.09 12.52 -4.32 -16.12 -10.84 -8.35 10.44
None 1.45 1.51 1.52 0.87 0.79 -1.49 -5.31 -4.82 1.30 4.42 0.44 0.73 1.31 1.56 3.97
Nuclear Periphery 1.35 -3.82 -4.93 -5.11 -0.47 -3.28 -4.03 -1.02 1.47 3.77 -2.91 -4.70 -5.12 -4.37 1.78
Nuclear Periphery Foci 1.74 -1.98 -1.39 -4.16 0.76 -2.49 -3.71 -0.83 -0.26 1.58 -0.68 -4.29 -2.85 -2.65 0.90
Nucleolus 1.77 -0.40 1.99 1.47 2.12 -2.73 -3.66 -0.47 2.04 3.47 -1.20 -3.64 1.07 2.45 3.99
Nucleus 0.85 -2.37 -0.14 -1.17 2.35 -2.95 -3.91 -1.08 1.25 3.41 -2.82 -4.53 -1.85 0.08 3.92
Peroxisomes 2.18 0.49 4.82 7.94 5.43 0.39 2.44 0.55 0.21 -1.87 0.41 2.09 4.22 4.93 2.33
Vacuole 0.19 -6.60 -6.56 -8.83 3.23 -3.85 -12.30 1.00 4.77 12.76 -2.90 -13.95 -6.77 -4.76 9.34
Vacuole Periphery 1.13 -3.25 -5.43 -10.92 -0.67 0.31 -5.78 2.46 3.22 8.34 1.64 -6.55 -3.87 -7.34 3.77
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rsm27

Rsm27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsm27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available