Standard name
Human Ortholog
Description ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.74 0.62 0.66 0.55 0.33 0.28 0.3 0.29 0.25 0.22 0.96 0.95 0.97 0.8 0.85 0.71 0.32 0.33 0.21 0.36 0.34 0.34
Endoplasmic Reticulum 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Endosome 0.07 0 0.16 0.05 0.17 0.05 0 0 0 0 0 0 0 0 0.1 0.06 0.11 0.14 0.11 0.1 0.05 0.06 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.21 0.19 0.43 0.36 0.73 0.78 0.75 0.83 0.85 0.9 0 0 0 0.09 0.09 0.08 0.34 0.34 0.49 0.4 0.34 0.29
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.06 0.08 0 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0.07 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 5 0 0 0 0 0 0 0 0 0 2 1 2 0 0 1 6 16 20 5 18 17
Bud 0 5 7 5 3 11 11 19 26 14 14 1 2 1 0 0 2 5 4 4 2 10 12
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 4 11 4 5 5 13
Bud Site 0 0 0 0 0 0 1 8 5 8 4 0 0 0 0 0 2
Cell Periphery 3 1 3 2 1 0 1 2 2 3 1 1 5 0 3 2 0 0 2 2 0 3 3
Cytoplasm 159 297 203 163 151 122 125 193 141 104 105 360 419 321 102 120 93 90 168 84 110 194 175
Endoplasmic Reticulum 10 21 2 1 0 3 2 6 3 2 3 4 11 3 3 12 5 4 7 9 6 11 8
Endosome 14 13 52 12 48 18 11 9 1 1 4 4 2 3 13 8 15 38 56 39 14 35 46
Golgi 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 5 0 20 4
Mitochondria 13 84 61 107 99 271 342 484 406 358 428 1 1 0 11 12 11 96 177 199 124 196 150
Nucleus 1 1 0 0 2 5 1 3 7 3 2 0 0 2 0 2 2 1 1 0 1 1 0
Nuclear Periphery 0 0 0 1 0 0 1 0 2 0 1 0 0 0 0 1 0 0 1 0 0 0 1
Nucleolus 0 1 1 0 0 1 3 1 1 2 0 0 0 0 3 2 3 0 0 0 0 0 0
Peroxisomes 0 0 2 1 4 1 0 3 1 0 0 1 0 0 0 0 0 0 0 2 0 5 4
SpindlePole 1 0 9 3 3 6 4 22 14 23 10 0 0 0 10 5 10 11 11 10 6 20 18
Vac/Vac Membrane 10 25 25 9 23 22 31 18 18 10 17 9 8 3 3 6 7 9 36 9 22 31 37
Unique Cell Count 210 402 329 246 276 373 441 648 491 423 475 374 442 330 128 141 131 281 515 409 307 569 513
Labelled Cell Count 220 453 366 304 334 461 533 768 627 528 589 383 449 335 149 171 154 281 515 409 307 569 513


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.0 4.6 4.2 4.2 3.4 3.2 3.4 2.9 2.9 3.0 6.2 6.3 6.5 5.3 5.0 6.3 5.9 6.2 6.5
Std Deviation (1e-4) 0.5 0.7 1.0 1.2 1.4 1.0 1.0 0.9 1.1 0.8 0.6 1.1 0.8 0.9 1.5 1.2 1.8 1.4 1.3 1.6
Intensity Change (Log2) -0.11 -0.13 -0.45 -0.53 -0.41 -0.63 -0.64 -0.6 0.44 0.48 0.5 0.23 0.14 0.47 0.38 0.43 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 -1.7 -7.7 -9.3 -9.6 -9.4 -10.3 -11.4 11.4 11.5 11.3 3.7 5.0 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 -7.2 -8.3 -6.9 -1.6 -3.0 -1.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -8.5 -9.2 -8.2 -2.6 -2.8 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.4 0 2.4
Vacuole -2.0 0.3 -0.9 -0.3 -3.5 -2.5 -3.4 -2.5 -3.2 -3.9 -4.3 -2.1 -1.3 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7751 2.037 2.1098 1.613 1.3998 2.123 3.2794 3.6515 3.6611 3.2535 3.1238 3.9761 3.3772 3.6359 3.5783 3.7056 3.8467 4.0215
Actin 0.0017 0.0019 0.0025 0.0008 0.0024 0.0015 0.0025 0.0047 0.0022 0.0057 0.002 0.002 0.0062 0.0013 0.0041 0.0027 0.0113 0.0054
Bud 0.0011 0.0015 0.0061 0.001 0.0024 0.0007 0.0027 0.0056 0.0015 0.002 0.0019 0.0006 0.0015 0.0033 0.0024 0.0057 0.0025 0.0007
Bud Neck 0.0019 0.0009 0.0008 0.0103 0.001 0.0024 0.0013 0.0073 0.0024 0.0017 0.0016 0.0064 0.0016 0.0011 0.0013 0.0011 0.0022 0.0027
Bud Periphery 0.0017 0.0017 0.0035 0.0015 0.0038 0.0012 0.002 0.0051 0.0021 0.0027 0.0023 0.0008 0.0021 0.0026 0.0047 0.0068 0.006 0.001
Bud Site 0.0057 0.0092 0.0048 0.0011 0.0018 0.0005 0.0048 0.0253 0.006 0.0012 0.0028 0.0009 0.0067 0.0114 0.0066 0.0034 0.0021 0.0006
Cell Periphery 0.0004 0.0007 0.0004 0.0003 0.0003 0.0002 0.001 0.0011 0.0007 0.0002 0.0004 0.0002 0.0008 0.0006 0.0007 0.0003 0.0006 0.0002
Cytoplasm 0.3919 0.5103 0.397 0.4325 0.4209 0.4438 0.5093 0.5557 0.5535 0.5772 0.4945 0.4978 0.5573 0.6236 0.559 0.6469 0.5427 0.534
Cytoplasmic Foci 0.0732 0.0554 0.0653 0.0728 0.1207 0.0993 0.09 0.0853 0.0731 0.088 0.1371 0.1013 0.0879 0.0967 0.0967 0.0855 0.0808 0.1056
Eisosomes 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0004 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0089 0.0085 0.0101 0.0079 0.0053 0.0064 0.006 0.0034 0.0048 0.0038 0.0043 0.0032 0.0075 0.0052 0.0039 0.0047 0.0035 0.0036
Endosome 0.0706 0.0718 0.0594 0.0837 0.109 0.0745 0.0597 0.0552 0.0393 0.0571 0.0912 0.0601 0.0588 0.0535 0.0628 0.0816 0.0493 0.0616
Golgi 0.0045 0.0057 0.0059 0.005 0.0179 0.005 0.0079 0.0096 0.0074 0.0077 0.0109 0.0069 0.0098 0.0099 0.0105 0.0089 0.0101 0.0088
Lipid Particles 0.0037 0.0022 0.0018 0.0039 0.0007 0.0015 0.002 0.0012 0.0013 0.0004 0.0024 0.0006 0.0013 0.0005 0.0009 0.0003 0.0011 0.0005
Mitochondria 0.0072 0.0143 0.0167 0.0122 0.0506 0.0197 0.0116 0.0161 0.019 0.0126 0.0145 0.0145 0.0143 0.0106 0.0329 0.0151 0.0188 0.0161
None 0.4023 0.2916 0.3963 0.3457 0.2349 0.3194 0.2806 0.2007 0.2705 0.2238 0.2075 0.2891 0.2265 0.1633 0.1945 0.1228 0.2511 0.2458
Nuclear Periphery 0.003 0.0027 0.0036 0.0045 0.0035 0.0023 0.0018 0.0011 0.0012 0.0014 0.0013 0.0008 0.0014 0.0009 0.001 0.0011 0.001 0.0009
Nucleolus 0.0008 0.0007 0.0008 0.0003 0.0005 0.0004 0.0005 0.0009 0.0004 0.0001 0.0004 0.0002 0.0004 0.0002 0.0003 0.0002 0.0006 0.0001
Nucleus 0.0051 0.0058 0.008 0.0036 0.0086 0.0058 0.0034 0.0068 0.0035 0.0033 0.0032 0.0023 0.004 0.0034 0.0028 0.0018 0.0045 0.002
Peroxisomes 0.0024 0.0022 0.0031 0.003 0.0041 0.0042 0.0034 0.0047 0.0037 0.0047 0.0077 0.0059 0.0039 0.0033 0.0055 0.0034 0.0061 0.0062
Punctate Nuclear 0.0033 0.0022 0.0039 0.0013 0.0022 0.0035 0.0016 0.0022 0.0016 0.0013 0.004 0.0022 0.0017 0.0015 0.0024 0.0009 0.0016 0.001
Vacuole 0.0087 0.0088 0.0078 0.0063 0.0065 0.006 0.0065 0.0065 0.0044 0.004 0.0082 0.0032 0.0052 0.0057 0.0054 0.0046 0.0032 0.0024
Vacuole Periphery 0.0018 0.0017 0.002 0.002 0.0025 0.0016 0.0012 0.0011 0.001 0.001 0.0016 0.001 0.0011 0.0011 0.0015 0.002 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2012 9.9634 8.379 9.9465 8.1887 5.941 9.7382 9.519 12.422 10.3499
Translational Efficiency 1.2173 0.6536 0.6991 0.6078 0.7709 1.186 0.9758 0.7389 0.6028 0.787

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
167 1534 284 1099 1832 906 122 2136 1999 2440 406 3235

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 840.23 814.40 952.45 884.42 693.97 788.44 943.81 914.97 706.19 804.76 949.85 904.59
Standard Deviation 99.84 108.71 102.13 110.65 81.53 101.59 101.63 121.71 92.53 106.86 102.06 118.95
Intensity Change Log 2 -0.045047 0.180859 0.073947 0.184128 0.443623 0.398851 0.063144 0.305670 0.230021

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001355 0.000603 0.000843 0.001891 0.000554 0.000886 0.003090 0.002662 0.000621 0.000708 0.001518 0.002400
Bud Neck 0.013955 0.024840 0.004935 0.025084 0.048561 0.056636 0.005251 0.032945 0.045670 0.036646 0.005030 0.030275
Bud Site 0.026529 0.028504 0.006801 0.062750 0.021828 0.031377 0.017821 0.056267 0.022221 0.029571 0.010112 0.058469
Cell Periphery 0.000206 0.000121 0.000313 0.000154 0.000162 0.000780 0.000406 0.000153 0.000166 0.000366 0.000341 0.000153
Cytoplasm 0.241791* 0.391188* 0.336291* 0.248603* 0.217652* 0.178436* 0.355950* 0.362536* 0.219669* 0.312191* 0.342198* 0.323830*
Cytoplasmic Foci 0.141913 0.257426* 0.076715 0.123807 0.366532* 0.331052* 0.054752 0.101293 0.347767* 0.284764* 0.070115 0.108942
Eisosomes 0.000132 0.000082 0.000148 0.000086 0.000327 0.000236 0.000133 0.000076 0.000311 0.000139 0.000143 0.000079
Endoplasmic Reticulum 0.002087 0.002065 0.007447 0.005670 0.002597 0.000919 0.011413 0.003229 0.002555 0.001640 0.008639 0.004059
Endosome 0.049356 0.030187 0.025939 0.043309 0.058926 0.099613 0.010477 0.022080 0.058127 0.055966 0.021293 0.029292
Golgi 0.010921 0.014377 0.000511 0.006853 0.033693 0.038993 0.001082 0.007845 0.031791 0.023517 0.000683 0.007508
Lipid Particles 0.003654 0.004559 0.011400 0.006208 0.017319 0.011812 0.005097 0.003720 0.016177 0.007252 0.009506 0.004565
Mitochondria 0.005212 0.022301 0.000564 0.011427 0.092887 0.083408 0.000496 0.015718 0.085563 0.044991 0.000544 0.014260
Mitotic Spindle 0.022137 0.012605 0.003244 0.107107* 0.006027 0.006760 0.002675 0.073420 0.007373 0.010434 0.003073 0.084864
None 0.000441 0.002545 0.003009 0.002965 0.001377 0.000802 0.002558 0.003626 0.001299 0.001897 0.002873 0.003402
Nuclear Periphery 0.000681 0.000569 0.003138 0.002462 0.000499 0.000242 0.001745 0.001854 0.000514 0.000447 0.002719 0.002060
Nuclear Periphery Foci 0.001025 0.000636 0.008346 0.003575 0.000252 0.000343 0.005736 0.001328 0.000316 0.000527 0.007562 0.002091
Nucleolus 0.001155 0.002330 0.003114 0.002694 0.001510 0.001729 0.000544 0.002838 0.001480 0.002107 0.002342 0.002789
Nucleus 0.066744 0.095436 0.275061* 0.075959 0.034492 0.024428 0.329198* 0.123189 0.037186 0.069070 0.291329* 0.107144
Peroxisomes 0.012635 0.015067 0.002242 0.028923 0.019275 0.045624 0.007835 0.020769 0.018720 0.026413 0.003922 0.023540
Vacuole 0.392918* 0.086790 0.227157* 0.235297* 0.066491 0.077673 0.183226 0.160617 0.093761 0.083404 0.213956* 0.185987
Vacuole Periphery 0.005155 0.007771 0.002784 0.005177 0.009038 0.008249 0.000515 0.003835 0.008713 0.007949 0.002102 0.004291

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.68 0.41 -2.60 -6.04 -3.51 -1.59 -2.54 -5.57 -4.17 0.59 -0.89 -2.80 -6.75 -6.74 -1.83
Bud Neck -5.10 3.28 -4.73 0.59 -11.68 -1.85 18.77 5.80 5.80 -16.66 3.42 19.70 6.79 3.17 -20.72
Bud Site -1.49 2.84 -4.92 -5.62 -12.22 -3.75 1.61 -9.51 -4.82 -7.40 -4.20 6.90 -11.50 -7.82 -16.69
Cell Periphery 1.10 -3.01 0.80 -1.21 8.19 -1.18 -3.41 2.76 1.26 3.94 -1.04 -6.69 3.18 1.22 8.05
Cytoplasm -6.97 -2.79 0.43 14.59 4.67 4.48 -4.48 -15.70 -17.75 -1.16 -11.96 -7.23 -12.44 0.57 0.59
Cytoplasmic Foci -11.73 4.77 1.77 22.34 -5.31 3.90 40.69 46.44 31.14 -7.04 9.10 43.80 44.89 37.74 -7.18
Eisosomes 3.64 -1.74 3.02 -1.59 6.25 2.05 4.22 6.23 9.13 3.98 4.51 4.23 6.20 8.06 8.09
Endoplasmic Reticulum 0.16 -8.63 -6.30 -6.75 3.49 4.14 -6.13 -1.86 -10.07 5.75 2.43 -9.34 -3.87 -9.86 7.81
Endosome 0.92 1.66 0.37 -1.47 -2.81 -7.95 18.91 16.30 17.52 -5.67 0.38 13.01 14.23 14.69 -1.56
Golgi -1.65 1.57 0.61 7.65 -7.51 -1.50 21.08 16.88 11.27 -5.60 4.32 21.44 17.32 12.92 -8.53
Lipid Particles -2.33 -6.19 -4.00 -2.15 3.91 3.37 10.25 12.80 6.20 2.11 8.27 5.54 11.98 4.72 5.33
Mitochondria -8.48 2.11 -2.36 7.04 -6.36 1.56 23.65 20.10 12.82 -13.00 9.55 23.43 20.22 13.78 -14.33
Mitotic Spindle 0.51 1.85 -9.13 -13.05 -14.57 -0.40 0.69 -16.54 -16.08 -16.41 -2.31 1.94 -21.00 -20.06 -22.25
None -5.77 -3.10 -2.20 0.36 0.37 1.27 -1.66 -3.01 -4.23 -0.58 -1.78 -2.28 -3.01 -1.83 -0.25
Nuclear Periphery 3.01 -7.02 -6.42 -8.49 1.18 1.95 -6.16 -6.68 -8.47 -0.26 0.68 -9.58 -9.08 -10.92 1.69
Nuclear Periphery Foci 2.65 -7.89 -6.03 -6.83 3.98 -1.03 -6.96 -7.79 -6.82 5.09 -3.18 -10.17 -9.91 -9.04 6.95
Nucleolus -3.83 -3.56 -3.96 -1.28 -0.34 -0.76 2.10 -3.61 -3.14 -10.50 -1.63 -1.29 -3.79 -3.41 -3.07
Nucleus -1.23 -11.14 -0.42 1.45 12.66 3.37 -13.03 -24.27 -25.10 8.36 -9.75 -20.83 -23.78 -13.03 13.61
Peroxisomes -2.39 3.08 -4.42 -3.42 -9.77 -8.58 5.44 1.00 8.94 -4.75 -5.07 12.67 -0.76 4.24 -12.78
Vacuole 15.42 6.68 5.54 -24.42 -2.68 -2.74 -7.97 -26.05 -20.40 1.36 2.54 -13.22 -24.97 -30.87 1.37
Vacuole Periphery -2.59 0.70 -0.02 6.54 -1.78 1.10 19.64 12.18 7.50 -8.52 1.30 11.93 11.82 10.20 -2.88
Standard name
Human Ortholog
Description ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
Localization
Cell Percentages mitochondrion (25%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Sal1

Sal1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sal1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available