Standard name
Human Ortholog
Description Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 1.0 0.98 0.96 0.91 – 0.84 0.83 0.75 0.76 0.99 0.99 0.99 0.92 0.94 0.95 0.94 0.87 0.86 0.82 0.74 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.11 0.13 – 0.38 0.33 0.46 0.4 0 0 0 0 0 0 0 0 0.07 0.06 0.1 0.12
Nucleus 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 6 0 1 6 10
Bud 0 3 5 0 0 1 1 – 7 5 6 2 3 2 0 0 0 0 2 2 0 4 11 12
Bud Neck 0 0 0 0 0 0 4 – 0 0 1 0 0 0 0 0 0 0 0 1 0 1 2 7
Bud Site 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 3 1 0 1 1 – 2 1 1 1 0 1 1 22 15 7 0 0 0 1 3 3
Cytoplasm 388 307 116 55 49 205 242 – 181 174 118 76 412 520 573 275 213 102 374 278 122 249 320 305
Endoplasmic Reticulum 3 2 0 0 0 0 1 – 0 0 1 0 2 1 3 11 11 9 2 2 0 6 14 7
Endosome 1 0 0 0 0 0 1 – 0 0 0 0 0 1 0 8 3 3 2 5 1 1 2 5
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 4 1 1 4 0 2 0 1
Mitochondria 5 6 2 1 5 23 35 – 82 70 72 40 5 2 1 7 3 1 4 4 10 18 45 51
Nucleus 0 0 0 0 1 4 7 – 4 11 4 2 0 0 3 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 0 0 0 – 1 0 1 0 0 1 0 1 1 0 1 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 1 0 0 1 4 0 0 2 4
Vac/Vac Membrane 1 1 0 0 0 1 2 – 0 2 0 0 0 0 1 19 5 4 1 3 0 11 16 22
Unique Cell Count 397 314 118 55 50 214 265 215 209 157 100 416 523 580 299 227 107 398 322 142 303 432 441
Labelled Cell Count 402 325 127 57 55 235 295 279 263 206 121 422 529 584 349 255 127 398 322 142 303 432 441


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.2 4.6 4.6 5.1 4.2 4.6 – 3.8 3.8 3.5 3.5 4.5 4.5 5.0 7.5 7.3 7.8 4.3 4.5 4.8
Std Deviation (1e-4) 0.5 0.9 0.6 1.3 1.6 1.6 1.8 – 1.6 1.7 1.2 0.5 1.0 0.8 1.4 1.3 1.4 2.3 1.4 1.7 1.5
Intensity Change (Log2) – – – 0.01 0.16 -0.14 0.01 – -0.28 -0.27 -0.41 -0.38 -0.02 -0.03 0.11 0.7 0.67 0.76 -0.09 -0.04 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 1.9 1.6 0
Cytoplasm 1.0 -0.1 -1.2 -2.6 – -4.0 -4.1 -5.3 -5.1 0.7 1.3 0.4 -2.4 -1.9 -1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 2.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2843 1.7622 2.0031 1.6462 1.9341 2.1142 0.4138 0.3115 0.2973 -0.2785 0.6382 0.1349 -0.2861 -0.264 -0.3328 -0.7793 -0.5281 -0.3826
Actin 0.0655 0.0041 0.0257 0.0329 0.0343 0.0113 0.0349 0.0009 0.0217 0.0399 0.0374 0.0042 0.0528 0.0009 0.0177 0.0001 0.0112 0.0018
Bud 0.0009 0.001 0.0003 0.0006 0.0029 0.0003 0.0017 0.003 0.0034 0.0004 0.0107 0.0001 0.002 0.003 0.0049 0.0002 0.0003 0.0008
Bud Neck 0.0089 0.0001 0.0027 0.0027 0.0053 0.0004 0.0015 0.0002 0.001 0.0007 0.0166 0.0005 0.0055 0.0039 0.0046 0.0001 0.0003 0.0009
Bud Periphery 0.0019 0.0022 0.0005 0.0012 0.0035 0.0014 0.0027 0.0036 0.0086 0.0003 0.0362 0.0002 0.0053 0.0035 0.0028 0.0001 0.0005 0.0029
Bud Site 0.0033 0.0034 0.0058 0.0011 0.0183 0.0001 0.0061 0.0064 0.0123 0.0017 0.0069 0.0002 0.0091 0.0095 0.0058 0.0008 0.0012 0.0022
Cell Periphery 0.0028 0.0006 0.0009 0.0002 0.0006 0.0002 0.0005 0.0004 0.0004 0.0001 0.0016 0.0001 0.0007 0.0002 0.0003 0 0.0001 0.0001
Cytoplasm 0.0817 0.1916 0.1296 0.1927 0.1933 0.2141 0.1886 0.4226 0.2565 0.4645 0.3367 0.4877 0.1849 0.2664 0.2723 0.2635 0.3148 0.379
Cytoplasmic Foci 0.0362 0.0085 0.0124 0.0372 0.0501 0.005 0.0516 0.0081 0.0134 0.0187 0.0078 0.0294 0.0175 0.0041 0.0098 0.0304 0.0296 0.0168
Eisosomes 0.0009 0.0002 0.0009 0.0003 0.0003 0.0019 0.0004 0.0002 0.0003 0.0002 0.0006 0 0.0007 0.0002 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0077 0.0017 0.001 0.0019 0.0017 0.0019 0.0082 0.0023 0.0065 0.0011 0.0049 0.003 0.0053 0.001 0.0038 0.0011 0.0017 0.0018
Endosome 0.011 0.0042 0.0023 0.0184 0.0232 0.0064 0.0413 0.0026 0.0188 0.0126 0.0367 0.0099 0.0183 0.001 0.0102 0.0125 0.0201 0.0057
Golgi 0.004 0.0003 0.003 0.006 0.008 0.0022 0.0173 0.0005 0.0066 0.0083 0.0155 0.0074 0.0067 0.0002 0.003 0.0005 0.0031 0.0006
Lipid Particles 0.0095 0.0006 0.0066 0.0163 0.0204 0.0037 0.0238 0.0003 0.0061 0.0038 0.0188 0.0024 0.0067 0.0002 0.0034 0.0003 0.0031 0.0011
Mitochondria 0.0052 0.0004 0.0031 0.0016 0.0022 0.0052 0.0175 0.0007 0.0113 0.0017 0.0459 0.0012 0.011 0.0002 0.0017 0.0003 0.0019 0.0007
None 0.7245 0.7771 0.7742 0.616 0.5869 0.7281 0.5369 0.5438 0.6145 0.4405 0.3405 0.4292 0.6422 0.7034 0.644 0.6817 0.5968 0.5757
Nuclear Periphery 0.0106 0.0004 0.0005 0.0067 0.0022 0.0071 0.0075 0.0005 0.0044 0.0003 0.0565 0.0168 0.0061 0.0002 0.0047 0.0007 0.0046 0.0033
Nucleolus 0.0011 0.0001 0.0009 0.0008 0.001 0.0022 0.001 0.0002 0.0003 0.0001 0.0019 0.0001 0.003 0.0001 0.0005 0.0004 0.0005 0.0002
Nucleus 0.0044 0.0004 0.0006 0.0012 0.0011 0.0023 0.0155 0.0009 0.0014 0.0005 0.0035 0.0047 0.0037 0.0005 0.0021 0.001 0.0013 0.0015
Peroxisomes 0.0058 0.0011 0.0152 0.0275 0.0296 0.0008 0.0294 0.0002 0.0062 0.0025 0.0044 0.0007 0.0055 0.0001 0.0017 0.0002 0.002 0.0005
Punctate Nuclear 0.0113 0.0006 0.0133 0.0316 0.0037 0.0041 0.0051 0.0008 0.0025 0.0004 0.0035 0.001 0.008 0.0004 0.0044 0.0022 0.0041 0.0024
Vacuole 0.002 0.0013 0.0005 0.0026 0.0104 0.0006 0.0064 0.0017 0.0031 0.0014 0.0066 0.001 0.0038 0.0006 0.0013 0.0033 0.002 0.0013
Vacuole Periphery 0.0007 0.0001 0.0001 0.0006 0.0008 0.0006 0.0022 0.0002 0.0008 0.0003 0.0068 0.0004 0.0014 0.0001 0.0005 0.0007 0.0007 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.2454 3.2116 3.5748 5.8099 5.5315 5.8349 6.799 7.8079 8.3659 9.4786
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1070 1375 259 1760 1479 2120 2162 392 2549 3495 2421 2152

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 665.47 731.11 915.61 832.00 651.09 750.43 820.65 814.66 657.13 742.83 830.81 828.84
Standard Deviation 69.16 113.65 98.51 102.62 108.75 99.04 93.36 111.22 94.44 105.45 98.41 104.45
Intensity Change Log 2 — 0.135715 0.460360 0.322210 — 0.204861 0.333910 0.323341 — 0.170324 0.399210 0.322769

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000055 0.000433 0.000552 0.000352 0.000103 0.000503 0.000071 0.000862 0.000083 0.000475 0.000123 0.000445
Bud Neck 0.023759 0.033577 0.002429 0.007188 0.015747 0.040058 0.002602 0.005938 0.019110 0.037508 0.002584 0.006960
Bud Site 0.002850 0.022185 0.005591 0.036425 0.005310 0.031367 0.005062 0.031248 0.004277 0.027755 0.005119 0.035482
Cell Periphery 0.000253 0.000105 0.000244 0.000155 0.000326 0.000118 0.000084 0.000125 0.000295 0.000113 0.000101 0.000150
Cytoplasm 0.501168* 0.453974* 0.337869* 0.551003* 0.524755* 0.432542* 0.622930* 0.536719* 0.514854* 0.440974* 0.592434* 0.548401*
Cytoplasmic Foci 0.208409* 0.263110* 0.046045 0.016871 0.226463* 0.258622* 0.020549 0.028051 0.218885* 0.260388* 0.023277 0.018908
Eisosomes 0.000283 0.000133 0.000109 0.000038 0.000407 0.000167 0.000030 0.000059 0.000355 0.000154 0.000038 0.000041
Endoplasmic Reticulum 0.000999 0.000693 0.006204 0.001551 0.001496 0.000515 0.000625 0.001779 0.001287 0.000585 0.001222 0.001593
Endosome 0.026282 0.033624 0.010126 0.003746 0.016025 0.038546 0.001847 0.007004 0.020331 0.036610 0.002733 0.004340
Golgi 0.002725 0.006352 0.000184 0.001642 0.002915 0.011597 0.000065 0.002802 0.002835 0.009534 0.000078 0.001854
Lipid Particles 0.012092 0.005934 0.005207 0.000854 0.010652 0.005280 0.000703 0.001353 0.011257 0.005537 0.001185 0.000945
Mitochondria 0.004973 0.006532 0.001287 0.003120 0.003303 0.012125 0.000896 0.005284 0.004004 0.009925 0.000937 0.003514
Mitotic Spindle 0.000904 0.003539 0.001384 0.032324 0.001383 0.009137 0.005129 0.019132 0.001182 0.006935 0.004729 0.029921
None 0.002625 0.002638 0.008659 0.003964 0.019069 0.000922 0.003189 0.004394 0.012166 0.001597 0.003774 0.004042
Nuclear Periphery 0.000398 0.000249 0.002291 0.001202 0.000382 0.000164 0.000922 0.000886 0.000389 0.000198 0.001068 0.001145
Nuclear Periphery Foci 0.000221 0.000472 0.004290 0.000860 0.000851 0.000246 0.000398 0.001356 0.000586 0.000335 0.000815 0.000950
Nucleolus 0.000706 0.001140 0.000776 0.000289 0.001778 0.000680 0.000256 0.000256 0.001328 0.000861 0.000312 0.000283
Nucleus 0.098537 0.048074 0.402371* 0.215374* 0.096737 0.032698 0.262588* 0.189887* 0.097493 0.038747 0.277542* 0.210732*
Peroxisomes 0.002960 0.011739 0.000872 0.000541 0.002764 0.010927 0.000367 0.003163 0.002846 0.011247 0.000421 0.001018
Vacuole 0.107860 0.103110 0.163148 0.120343 0.068350 0.110192 0.071076 0.157693 0.084935 0.107406 0.080926 0.127147
Vacuole Periphery 0.001942 0.002388 0.000361 0.002156 0.001184 0.003592 0.000610 0.002008 0.001502 0.003118 0.000583 0.002129

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.95 -7.18 -7.69 1.11 3.77 -3.41 1.25 -3.23 -0.22 -3.40 -5.08 -3.68 -7.65 1.15 -5.81
Bud Neck -3.42 11.45 9.65 12.45 -4.53 -11.52 10.94 8.23 18.58 -2.11 -10.80 15.80 12.20 22.10 -5.64
Bud Site -11.96 -1.78 -8.51 0.96 -5.94 -13.86 1.18 -3.00 4.15 -3.28 -17.98 -0.16 -8.37 4.14 -7.92
Cell Periphery 8.26 -0.10 7.19 -1.58 6.72 4.70 5.64 4.71 0.21 -4.93 6.81 7.43 6.52 -0.72 -3.11
Cytoplasm 4.58 13.80 6.90 1.89 -9.81 10.24 -3.44 9.35 3.74 11.37 10.84 -1.32 11.84 2.34 12.16
Cytoplasmic Foci -6.86 27.23 37.77 42.00 5.96 -4.70 41.00 37.09 45.17 -1.43 -7.98 53.40 55.43 65.62 4.87
Eisosomes 9.45 10.34 18.50 8.19 9.51 4.24 6.63 5.84 9.11 -9.04 6.03 9.40 9.12 14.83 -4.30
Endoplasmic Reticulum 1.86 -9.40 -7.41 -12.49 7.80 2.80 1.36 -4.16 -10.66 -8.71 3.27 -2.46 -5.43 -17.19 -4.38
Endosome -2.88 8.33 13.60 17.04 7.19 -12.19 12.84 7.79 18.57 -1.46 -10.73 17.84 17.47 28.82 0.33
Golgi -3.98 3.79 2.67 8.04 -1.08 -9.77 10.41 3.58 10.95 -1.73 -10.25 8.52 4.54 13.36 -1.74
Lipid Particles 5.82 6.14 11.64 9.17 7.15 5.74 11.35 10.03 8.52 -5.62 8.16 15.50 15.58 13.83 0.17
Mitochondria -0.90 2.68 2.49 4.36 -0.46 -6.95 4.70 1.15 6.14 -0.93 -5.80 4.95 3.09 8.65 -1.47
Mitotic Spindle -3.06 -0.70 -8.54 -7.15 -8.40 -4.78 -2.95 -2.56 -0.59 -1.56 -5.57 -3.41 -8.72 -5.52 -6.43
None -0.01 -0.89 -1.64 -1.58 0.50 6.66 5.94 5.60 -4.92 -1.30 6.48 5.52 5.21 -5.94 -1.56
Nuclear Periphery 3.12 -15.96 -22.38 -25.31 2.34 5.13 -18.16 -11.52 -13.72 -2.93 6.04 -23.28 -25.48 -29.15 -8.93
Nuclear Periphery Foci -4.43 -8.94 -11.87 -7.14 7.16 3.21 1.72 -4.43 -8.60 -7.35 2.23 -2.84 -5.18 -11.03 -2.58
Nucleolus -3.03 -0.00 6.88 5.51 5.50 2.88 4.04 3.89 3.32 -0.76 1.93 4.60 4.58 6.45 -0.25
Nucleus 12.88 -17.30 -26.40 -36.64 7.88 18.82 -31.86 -11.94 -17.79 3.74 22.94 -37.11 -30.00 -43.53 5.65
Peroxisomes -8.61 4.72 6.12 12.45 2.83 -10.95 9.22 2.37 10.44 -1.45 -13.87 10.08 9.14 18.88 -0.87
Vacuole 0.21 -7.95 -15.59 -16.51 0.00 -10.68 -10.56 -16.12 -13.22 -12.79 -7.29 -9.73 -25.07 -21.12 -16.53
Vacuole Periphery -1.32 6.64 2.47 3.58 -1.44 -7.94 3.62 1.10 5.41 -0.52 -7.17 5.35 2.02 6.20 -1.20
Standard name
Human Ortholog
Description Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (71%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sap4

Sap4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sap4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available