Standard name
Human Ortholog
Description Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.1 0.09 0.11 0.15 0.16 0.13 0.12 0.06 0.15 0.18 0.11 0.11 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.06 0 0 0.1 0.09 0.1 0 0.12 0 0.09 0.05 0 0.07 0 0.16 0.18 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.8 0.74 0.8 0.73 0.52 0.46 0.39 0.48 0.42 0.46 0.47 0.33 0.55 0.55 0.43 0.58 0.56 0.42 0.82 0.8 0.79 0.74 0.65 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0.06 0.05 0.07 0 0.07 0 0.13 0.05 0.12 0.12 0.08 0 0 0.05 0 0 0.05 0.06 0.09 0.08 0.11 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.23 0.13 0.2 0.36 0.39 0.46 0.36 0.32 0.29 0.29 0.38 0.31 0.28 0.34 0.23 0.21 0.36 0.08 0.1 0.08 0.12 0.2 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 0 2 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 0 1 1 1 1 1 0 0 0 0 0 0 0 9 3 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 2 6 5 17 24 33 19 37 29 22 7 21 30 39 34 34 3 2 1 1 1 3
Endoplasmic Reticulum 0 0 0 0 1 2 0 1 0 0 0 1 0 1 0 13 13 13 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0 2
Golgi 3 5 10 2 2 17 23 30 1 29 9 16 6 5 12 15 50 45 0 0 0 0 0 3
Mitochondria 0 2 0 0 0 0 2 0 4 0 0 1 0 0 0 6 3 2 0 1 0 1 1 0
Nucleus 277 239 131 114 60 81 104 144 53 108 103 59 61 77 70 197 173 106 346 352 160 89 98 85
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2 1 0 3
Nucleolus 10 16 9 8 8 4 18 12 17 12 26 21 9 2 7 17 11 6 21 27 19 10 17 19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Vac/Vac Membrane 60 75 21 31 41 69 125 108 40 69 62 68 34 39 55 80 65 91 33 44 15 14 29 44
Unique Cell Count 345 325 163 157 115 177 269 302 126 237 217 177 111 139 163 341 307 250 421 443 203 121 151 165
Labelled Cell Count 354 342 173 161 118 190 299 329 136 258 233 189 117 145 174 367 350 297 421 443 203 121 151 165


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 46.4 34.4 40.1 37.3 26.4 39.3 36.6 36.8 31.4 34.0 32.3 31.0 46.8 45.3 43.2 37.6 46.8 47.0 41.8 41.6 41.0
Std Deviation (1e-4) 12.1 8.5 7.3 8.0 10.3 7.7 8.0 8.4 8.2 8.6 8.8 8.6 12.0 14.4 13.1 12.6 15.1 18.1 12.8 13.3 13.2
Intensity Change (Log2) – – – -0.1 -0.6 -0.03 -0.13 -0.13 -0.36 -0.24 -0.31 -0.37 0.22 0.18 0.11 -0.09 0.22 0.23 0.06 0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.4 3.3 3.8 4.5 4.8 4.3 4.0 0 4.5 5.2 3.9 3.8 4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.3 0 1.2 0.9 1.4 0 2.0 -0.9 1.0 -0.3 -1.0 0.4 -0.8 3.1 3.5
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.6 -5.0 -6.6 -8.4 -6.8 -6.7 -7.0 -6.5 -8.7 -4.5 -4.7 -6.9 -5.0 -5.2 -7.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 0.5 -1.6 0.5 -0.8 2.3 -0.2 2.2 2.1 0.8 -1.9 -0.5 -0.3 -1.0 -1.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.7 4.5 5.4 7.2 5.3 3.9 3.8 3.7 5.4 3.6 3.3 4.5 2.8 2.2 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.1222 40.7754 36.1333 36.1814 37.216 37.1078 41.9296 44.1209 43.7532 38.3112 44.1519 45.0415 42.7339 46.0173 44.3191 44.6845 42.7783 42.1799
Actin 0.0071 0 0.0078 0.0328 0 0.0001 0.005 0.0001 0.0004 0.0027 0.0011 0.0001 0.0042 0 0.0023 0 0.0001 0.0001
Bud 0.0011 0 0.0001 0.0001 0 0 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0 0 0.0001
Bud Neck 0.0003 0.0001 0.0001 0.0001 0.0003 0.0008 0.0005 0.0001 0.0001 0.0001 0.0006 0.0006 0.0001 0.0001 0.0001 0 0.0001 0.001
Bud Periphery 0.0011 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0002
Bud Site 0.0012 0 0.0002 0.0003 0 0 0.0008 0.0001 0.0002 0.0004 0.0001 0 0.0002 0.0001 0.0013 0 0.0001 0
Cell Periphery 0.0006 0 0.0002 0 0.0006 0.0003 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0.0003
Cytoplasm 0.0054 0.0028 0.0011 0.0003 0.0003 0.0003 0.0056 0.0016 0.0024 0.0016 0.0039 0.0003 0.0011 0.0007 0.0005 0.0003 0.0021 0.0006
Cytoplasmic Foci 0.0126 0 0.0059 0.0007 0 0 0.0135 0.0001 0.0004 0.0054 0.001 0.0001 0.0047 0.0038 0.0002 0 0.0058 0.0023
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0015 0 0.0003 0 0 0.0001 0.0008 0.0001 0.0001 0.0014 0.0007 0.0003 0.0003 0 0 0 0.0001 0.0001
Endosome 0.0075 0 0.0023 0.0002 0 0 0.0089 0 0.0004 0.0062 0.001 0.0041 0.0045 0.0001 0.0001 0 0.0003 0.0003
Golgi 0.0039 0 0.001 0.001 0 0 0.0027 0 0 0.0024 0.0002 0.0003 0.0012 0 0.0002 0 0.0001 0.0001
Lipid Particles 0.0131 0 0.0041 0.0011 0 0 0.0054 0 0.0002 0.0053 0.0002 0.0002 0.0042 0.0013 0.0003 0 0.0037 0.0007
Mitochondria 0.0108 0.0002 0.0061 0.0013 0.0003 0.0003 0.0019 0.0005 0.0019 0.0019 0.0009 0.0005 0.0019 0.0009 0.0011 0.0002 0.0005 0.0003
None 0.0022 0 0.0007 0 0 0 0.0019 0.0001 0.0004 0.0004 0.0003 0 0.0008 0.0002 0.0001 0 0.0017 0.0006
Nuclear Periphery 0.0225 0.0256 0.0187 0.018 0.0089 0.0214 0.0254 0.0244 0.0278 0.0432 0.0348 0.0085 0.0092 0.008 0.0048 0.0029 0.0086 0.0074
Nucleolus 0.0391 0.0097 0.0189 0.003 0.0597 0.0382 0.0331 0.0082 0.0206 0.0213 0.0573 0.0176 0.0245 0.0073 0.0055 0.0024 0.0179 0.0212
Nucleus 0.8554 0.9602 0.9232 0.9379 0.926 0.9361 0.8828 0.9574 0.9389 0.892 0.8853 0.9638 0.9369 0.9764 0.9818 0.994 0.9521 0.9574
Peroxisomes 0.002 0 0.0046 0.0027 0 0 0.0026 0 0.0001 0.0007 0 0 0.0018 0.0003 0.0003 0 0.0001 0
Punctate Nuclear 0.0042 0.0001 0.001 0.0001 0.0002 0.0002 0.0051 0.0064 0.0033 0.0084 0.0086 0.0002 0.001 0.0003 0.0002 0 0.0056 0.005
Vacuole 0.0041 0.0009 0.0014 0.0001 0.0023 0.0011 0.0013 0.0003 0.0009 0.0018 0.0014 0.0016 0.0015 0.0002 0.0004 0 0.0005 0.0018
Vacuole Periphery 0.0041 0.0004 0.0018 0.0002 0.0012 0.0009 0.0019 0.0006 0.0014 0.0041 0.0022 0.0016 0.0015 0.0003 0.0003 0 0.0008 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 155.5524 109.1642 163.9377 158.8188 131.9405 132.219 166.5492 168.5033 158.0918 144.5308
Translational Efficiency 1.3612 1.9754 1.2777 1.4902 1.4095 1.7867 1.4321 1.4164 1.3701 1.3628

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
439 564 125 1736 1677 1534 1324 1810 2116 2098 1449 3546

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2063.66 2805.45 3590.92 3124.40 2495.24 2938.92 3406.32 3169.33 2405.70 2903.04 3422.24 3147.33
Standard Deviation 463.21 671.65 570.68 751.68 529.57 602.69 696.77 679.01 545.35 624.79 688.76 715.86
Intensity Change Log 2 — 0.443027 0.799148 0.598374 — 0.236108 0.449035 0.344999 — 0.333463 0.618100 0.465230

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001379 0.000882 0.001658 0.000771 0.000951 0.000788 0.001226 0.000820 0.001040 0.000813 0.001263 0.000796
Bud Neck 0.028730 0.014726 0.027010 0.022435 0.017979 0.015854 0.027853 0.020520 0.020210 0.015551 0.027780 0.021458
Bud Site 0.002020 0.000740 0.001793 0.002850 0.000684 0.000721 0.001142 0.001183 0.000961 0.000726 0.001198 0.001999
Cell Periphery 0.000296 0.000111 0.000112 0.000225 0.000147 0.000139 0.000100 0.000105 0.000178 0.000132 0.000101 0.000164
Cytoplasm 0.002740 0.001384 0.000386 0.003693 0.001822 0.000822 0.000757 0.002095 0.002012 0.000973 0.000725 0.002877
Cytoplasmic Foci 0.000722 0.000308 0.001541 0.000841 0.000298 0.000268 0.000585 0.000462 0.000386 0.000279 0.000667 0.000647
Eisosomes 0.000039 0.000038 0.000094 0.000026 0.000031 0.000031 0.000053 0.000028 0.000033 0.000033 0.000057 0.000027
Endoplasmic Reticulum 0.001658 0.000563 0.000499 0.000672 0.001598 0.001459 0.000624 0.000750 0.001610 0.001218 0.000614 0.000712
Endosome 0.000201 0.000063 0.000055 0.000821 0.000086 0.000163 0.000152 0.000341 0.000110 0.000136 0.000144 0.000576
Golgi 0.000104 0.000120 0.000253 0.000656 0.000080 0.000114 0.000148 0.000367 0.000085 0.000115 0.000157 0.000508
Lipid Particles 0.000038 0.000025 0.000039 0.000071 0.000016 0.000278 0.000762 0.000022 0.000021 0.000210 0.000700 0.000046
Mitochondria 0.003860 0.002327 0.005715 0.002985 0.002700 0.002030 0.004616 0.002742 0.002941 0.002110 0.004711 0.002861
Mitotic Spindle 0.000041 0.000362 0.005281 0.006075 0.000023 0.000992 0.000465 0.001798 0.000027 0.000823 0.000881 0.003892
None 0.002321 0.001301 0.000892 0.003878 0.001319 0.001190 0.001050 0.001405 0.001527 0.001220 0.001037 0.002616
Nuclear Periphery 0.001817 0.000425 0.006257 0.001493 0.001530 0.001264 0.001325 0.001043 0.001590 0.001039 0.001750 0.001263
Nuclear Periphery Foci 0.000519 0.000259 0.000494 0.000806 0.000220 0.000161 0.000080 0.000368 0.000282 0.000187 0.000116 0.000582
Nucleolus 0.032314 0.031239 0.080866 0.016867 0.045059 0.037536 0.046512 0.025883 0.042415 0.035843 0.049475 0.021469
Nucleus 0.919351* 0.941028* 0.858742* 0.932883* 0.923276* 0.934084* 0.910004* 0.938919* 0.922462* 0.935950* 0.905582* 0.935964*
Peroxisomes 0.000490 0.000548 0.006635 0.000418 0.000537 0.000412 0.001592 0.000446 0.000527 0.000449 0.002027 0.000432
Vacuole 0.000901 0.001933 0.000966 0.001133 0.000702 0.000771 0.000545 0.000410 0.000743 0.001083 0.000582 0.000764
Vacuole Periphery 0.000459 0.001620 0.000711 0.000403 0.000943 0.000922 0.000408 0.000293 0.000843 0.001110 0.000434 0.000347

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.62 -2.82 7.23 0.69 6.86 3.70 -8.74 1.06 -2.74 9.73 5.70 -7.91 4.67 -1.89 12.62
Bud Neck 5.43 -0.45 -0.09 -8.35 0.46 1.97 -11.74 -6.51 -8.51 5.51 4.74 -10.07 -6.90 -12.52 4.42
Bud Site 4.31 -1.24 -2.31 -5.36 -1.01 -0.42 -9.71 -3.30 -2.98 -0.31 2.11 -6.38 -5.56 -6.27 -3.07
Cell Periphery 4.07 3.53 1.81 -2.98 -2.20 0.25 1.15 1.33 0.90 0.43 1.91 2.85 0.68 -1.31 -2.17
Cytoplasm 0.69 3.58 -2.17 -2.58 -6.66 1.89 2.04 -0.55 -2.48 -2.64 1.80 2.89 -2.26 -4.68 -6.23
Cytoplasmic Foci 2.10 -2.73 -1.01 -1.99 1.55 0.35 -3.04 -1.10 -1.02 1.35 0.85 -3.34 -1.97 -2.20 0.18
Eisosomes 0.20 -7.23 3.66 2.73 8.58 0.32 -12.43 0.49 0.04 12.98 0.12 -13.65 2.40 1.65 15.78
Endoplasmic Reticulum 5.69 5.43 5.05 -2.55 -1.66 0.51 3.74 3.41 5.92 -1.46 1.85 4.75 4.42 5.54 -1.85
Endosome 3.63 4.31 -1.42 -2.02 -2.02 -1.32 -0.56 -0.94 -0.56 -0.71 -0.60 -0.15 -1.90 -1.68 -1.70
Golgi -0.54 -3.87 -1.51 -1.33 -0.63 -0.58 -3.08 -1.13 -0.96 -0.72 -0.78 -3.63 -1.93 -1.66 -1.32
Lipid Particles 0.16 -1.42 -1.96 -1.93 -1.10 -0.98 -1.03 -0.98 0.94 1.01 -0.98 -1.03 -2.49 0.73 0.92
Mitochondria 5.50 -3.52 1.57 -5.94 4.37 5.06 -13.04 -2.93 -7.60 10.66 7.00 -12.55 -2.91 -10.46 10.97
Mitotic Spindle -1.34 -1.28 -5.07 -4.53 0.37 -1.47 -1.54 -3.14 -1.86 -2.20 -1.72 -1.90 -5.94 -4.31 -3.03
None 1.38 1.68 -1.82 -5.63 -6.16 0.65 1.34 -1.99 -2.11 -2.96 1.46 2.03 -4.29 -5.83 -6.62
Nuclear Periphery 2.64 -0.90 -0.01 -7.02 0.91 0.47 0.41 1.06 0.42 0.63 1.55 -0.23 0.36 -1.40 0.48
Nuclear Periphery Foci 0.82 0.43 -0.62 -1.30 -0.95 0.85 2.41 -0.86 -1.12 -1.44 1.03 2.74 -1.53 -1.98 -2.56
Nucleolus 0.19 -3.94 3.39 3.67 5.40 2.13 -0.65 5.31 3.12 5.70 2.23 -2.20 7.65 5.50 8.90
Nucleus -2.95 3.82 -0.27 3.41 -4.05 -2.48 4.91 -1.11 1.37 -5.95 -3.59 5.85 -0.49 3.35 -6.53
Peroxisomes -0.77 -4.98 0.49 1.09 5.00 1.40 -7.80 0.22 -1.23 7.89 0.96 -9.05 0.76 -0.49 9.37
Vacuole -1.30 -2.34 -2.16 0.45 -0.66 -0.70 -1.09 2.25 2.07 1.90 -1.51 -1.16 -1.94 0.20 -0.41
Vacuole Periphery -1.58 -1.53 1.26 1.75 2.25 -0.00 3.77 4.53 2.43 2.73 -0.92 3.60 4.42 2.91 2.24
Standard name
Human Ortholog
Description Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
Localization
Cell Percentages nucleus (71%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Scl1

Scl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available