Standard name | |
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Human Ortholog | |
Description | Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 3 | 9 | 1 | 2 | 6 | 5 |
Bud | 7 | 4 | 8 | 9 | 5 | 2 | – | 3 | 6 | 5 | 8 | 6 | 2 | 2 | 0 | 2 | 9 | 5 | 4 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 2 |
Bud Site | 2 | 1 | 2 | 0 | 2 | 0 | – | 4 | 3 | 3 | 4 | 5 | 0 | 0 | 0 | 1 | 2 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 11 | 0 | 0 | 1 | 2 | – | 0 | 0 | 2 | 1 | 2 | 5 | 3 | 1 | 12 | 11 | 20 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 27 | 21 | 8 | 6 | 2 | 6 | – | 8 | 5 | 7 | 3 | 4 | 24 | 12 | 12 | 18 | 19 | 22 | 5 | 2 | 1 | 4 | 3 | 3 |
Endoplasmic Reticulum | 3 | 4 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 2 | 1 | 7 | 2 | 5 | 5 | 4 | 10 | 3 | 2 | 0 | 2 | 1 | 1 |
Endosome | 5 | 30 | 1 | 0 | 11 | 0 | – | 0 | 0 | 1 | 1 | 0 | 14 | 17 | 14 | 30 | 15 | 18 | 18 | 27 | 18 | 8 | 15 | 15 |
Golgi | 0 | 13 | 0 | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 28 | 17 | 13 | 26 | 13 | 11 | 4 | 14 | 2 | 8 | 10 | 10 |
Mitochondria | 237 | 198 | 130 | 120 | 145 | 115 | – | 176 | 198 | 185 | 221 | 249 | 138 | 97 | 102 | 17 | 11 | 16 | 208 | 246 | 99 | 116 | 106 | 111 |
Nucleus | 5 | 2 | 3 | 0 | 1 | 0 | – | 2 | 3 | 1 | 2 | 1 | 0 | 1 | 1 | 6 | 7 | 8 | 0 | 0 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 1 | 4 | 4 | 2 | 4 | 13 | – | 17 | 13 | 12 | 16 | 23 | 1 | 2 | 2 | 2 | 1 | 1 | 2 | 1 | 0 | 3 | 0 | 2 |
Nucleolus | 2 | 2 | 1 | 2 | 7 | 6 | – | 18 | 14 | 14 | 14 | 27 | 1 | 0 | 0 | 3 | 5 | 5 | 1 | 0 | 0 | 1 | 0 | 3 |
Peroxisomes | 7 | 9 | 1 | 1 | 5 | 0 | – | 1 | 0 | 1 | 2 | 2 | 7 | 7 | 8 | 49 | 24 | 44 | 8 | 14 | 11 | 11 | 14 | 10 |
SpindlePole | 6 | 2 | 10 | 0 | 8 | 1 | – | 5 | 1 | 2 | 5 | 4 | 1 | 2 | 2 | 15 | 20 | 15 | 3 | 2 | 2 | 2 | 4 | 6 |
Vac/Vac Membrane | 49 | 63 | 5 | 2 | 10 | 3 | – | 4 | 5 | 6 | 3 | 4 | 18 | 9 | 21 | 37 | 28 | 37 | 18 | 18 | 2 | 3 | 8 | 15 |
Unique Cell Count | 270 | 279 | 138 | 126 | 155 | 128 | 191 | 206 | 201 | 231 | 262 | 197 | 135 | 150 | 177 | 137 | 166 | 290 | 351 | 149 | 170 | 179 | 198 | |
Labelled Cell Count | 354 | 364 | 173 | 143 | 203 | 149 | 238 | 248 | 242 | 282 | 328 | 248 | 172 | 181 | 223 | 171 | 214 | 290 | 351 | 149 | 170 | 179 | 198 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.8 | 3.8 | 4.3 | 5.7 | 5.6 | – | 5.4 | 5.2 | 5.3 | 5.2 | 5.1 | 5.7 | 6.1 | 6.2 | 7.6 | 7.9 | 7.3 | 5.8 | 6.2 | 6.2 |
Std Deviation (1e-4) | 1.0 | 1.1 | 0.8 | 1.1 | 1.7 | 1.9 | – | 2.3 | 1.8 | 1.8 | 1.7 | 1.7 | 1.1 | 1.3 | 1.3 | 1.9 | 2.6 | 1.9 | 1.6 | 2.2 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.21 | 0.61 | 0.58 | – | 0.53 | 0.46 | 0.5 | 0.47 | 0.45 | 0.61 | 0.71 | 0.72 | 1.02 | 1.06 | 0.97 | 0.63 | 0.73 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -1.1 | 0 | – | 0 | -1.3 | -1.6 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0.3 | -1.2 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.4 | 4.2 |
Cytoplasm | -0.4 | 0 | -0.4 | – | -0.7 | -1.6 | -1.0 | 0 | 0 | 2.0 | 1.0 | 0.7 | 1.4 | 2.2 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.8 | 4.0 | 3.3 | 4.8 | 3.6 | 3.6 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.6 | 4.3 | 3.5 | 4.7 | 3.7 | 0 |
Mitochondria | 0 | -0.2 | -1.3 | – | -0.7 | 0.8 | -0.8 | 0.6 | 0.4 | -5.4 | -4.9 | -5.6 | -14.9 | -14.3 | -14.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.4 | – | 2.2 | 1.4 | 1.3 | 1.7 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 3.3 | 2.7 | 2.7 | 2.5 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.5 | 4.8 | 6.3 |
SpindlePole | 0 | -0.7 | -2.6 | – | -2.0 | 0 | 0 | -2.4 | 0 | 0 | -2.3 | -2.5 | 0.4 | 2.0 | 0.6 |
Vacuole | 0 | 1.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.1 | 3.1 | 4.5 | 4.3 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9287 | 3.0509 | 2.9169 | 2.4119 | 3.1558 | 2.9544 | 1.6829 | 1.7584 | 1.5431 | 1.7037 | 1.5848 | 1.698 | 4.5124 | 4.8271 | 4.3566 | 4.1468 | 4.3036 | 4.4292 |
Actin | 0.0027 | 0.0018 | 0.0011 | 0.0002 | 0.0004 | 0.0008 | 0.0313 | 0.012 | 0.0175 | 0.0087 | 0.0073 | 0.0085 | 0.0023 | 0.0098 | 0.004 | 0.0014 | 0.0018 | 0.0021 |
Bud | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0019 | 0.0015 | 0.0045 | 0.0017 | 0.0007 | 0.0003 | 0.0009 | 0.0005 | 0.0002 | 0.0001 | 0.0004 |
Bud Neck | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0006 | 0.0006 | 0.002 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0013 | 0.0018 | 0.0031 | 0.0056 | 0.0047 | 0.0016 | 0.0009 | 0.0024 | 0.0026 | 0.0012 | 0.0003 | 0.0022 |
Bud Site | 0.0006 | 0.0012 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0024 | 0.0171 | 0.0085 | 0.0268 | 0.0007 | 0.001 | 0.0007 | 0.0017 | 0.0004 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0005 |
Cytoplasm | 0.0009 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0042 | 0.0016 | 0.0025 | 0.0014 | 0.0014 | 0.0032 | 0.0045 | 0.0006 | 0.0005 | 0.0002 | 0.0004 | 0.0006 |
Cytoplasmic Foci | 0.0078 | 0.0125 | 0.0075 | 0.0021 | 0.0033 | 0.0066 | 0.0219 | 0.0288 | 0.0192 | 0.0242 | 0.0107 | 0.0266 | 0.012 | 0.0106 | 0.0073 | 0.0117 | 0.0177 | 0.0091 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0016 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0029 | 0.0007 | 0.0007 | 0.0004 | 0.0007 | 0.0025 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 |
Endosome | 0.0273 | 0.0248 | 0.0179 | 0.0088 | 0.022 | 0.0126 | 0.054 | 0.0498 | 0.031 | 0.0226 | 0.0481 | 0.0183 | 0.0264 | 0.0322 | 0.0194 | 0.0254 | 0.0186 | 0.0193 |
Golgi | 0.0276 | 0.0661 | 0.0158 | 0.0123 | 0.0263 | 0.0218 | 0.0472 | 0.0702 | 0.031 | 0.0427 | 0.0643 | 0.0338 | 0.0353 | 0.0462 | 0.0288 | 0.0279 | 0.0471 | 0.0293 |
Lipid Particles | 0.0114 | 0.0071 | 0.0046 | 0.0011 | 0.0015 | 0.01 | 0.0351 | 0.0155 | 0.0045 | 0.0176 | 0.0305 | 0.0075 | 0.0297 | 0.0063 | 0.0025 | 0.0148 | 0.0034 | 0.0101 |
Mitochondria | 0.8856 | 0.8582 | 0.926 | 0.9658 | 0.9325 | 0.9215 | 0.7207 | 0.7544 | 0.8233 | 0.7985 | 0.8085 | 0.8531 | 0.845 | 0.8554 | 0.9038 | 0.8601 | 0.8431 | 0.9008 |
None | 0.001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0051 | 0.0003 | 0.0092 | 0.0008 | 0.001 | 0.0017 | 0.0018 | 0.0012 | 0.0004 | 0.0002 | 0.0001 | 0.0013 |
Nuclear Periphery | 0.001 | 0.0016 | 0.0001 | 0 | 0 | 0.0007 | 0.0169 | 0.0003 | 0.0035 | 0.0003 | 0.0004 | 0.0029 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0009 |
Nucleolus | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0012 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 |
Nucleus | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.006 | 0.0002 | 0.0005 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 |
Peroxisomes | 0.0261 | 0.0207 | 0.0167 | 0.0076 | 0.0101 | 0.0216 | 0.0366 | 0.0339 | 0.0332 | 0.0336 | 0.0132 | 0.0317 | 0.0303 | 0.024 | 0.0247 | 0.0508 | 0.0658 | 0.0198 |
Punctate Nuclear | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0017 | 0.0003 | 0.0033 | 0.0025 | 0.0003 | 0.0013 | 0.0002 | 0.0021 | 0 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0028 | 0.0013 | 0.004 | 0.0005 | 0.0012 | 0.0009 | 0.0057 | 0.0048 | 0.0028 | 0.0025 | 0.0022 | 0.0014 | 0.0028 | 0.0028 | 0.0019 | 0.0024 | 0.0003 | 0.0009 |
Vacuole Periphery | 0.0027 | 0.0027 | 0.0026 | 0.0011 | 0.0018 | 0.0019 | 0.0042 | 0.0039 | 0.0019 | 0.0026 | 0.0031 | 0.0024 | 0.0052 | 0.0024 | 0.0019 | 0.0022 | 0.0006 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.154 | 4.0343 | 10.4776 | 13.2783 | 5.8512 | 27.0624 | 17.8458 | 21.3363 | 26.586 | 20.8738 |
Translational Efficiency | 3.0612 | 4.9308 | 2.4482 | 2.9355 | 4.9703 | 1.946 | 2.2933 | 2.4158 | 2.0914 | 2.3417 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1491 | 1330 | 293 | 1081 | 1736 | 2214 | 1870 | 838 | 3227 | 3544 | 2163 | 1919 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 592.02 | 751.89 | 1157.70 | 917.95 | 713.00 | 777.18 | 911.16 | 926.20 | 657.10 | 767.69 | 944.56 | 921.55 |
Standard Deviation | 86.69 | 108.54 | 173.25 | 153.04 | 81.60 | 104.27 | 121.08 | 170.37 | 103.40 | 106.60 | 154.46 | 160.89 |
Intensity Change Log 2 | — | 0.344876 | 0.967544 | 0.632770 | — | 0.124347 | 0.353802 | 0.377422 | — | 0.228583 | 0.664764 | 0.498884 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000438 | 0.002993 | 0.005612 | 0.007248 | 0.000979 | 0.000490 | 0.005480 | 0.031793 | 0.000729 | 0.001429 | 0.005497 | 0.017967 |
Bud Neck | 0.046495 | 0.007928 | 0.007417 | 0.005884 | 0.005218 | 0.007732 | 0.008123 | 0.004060 | 0.024290 | 0.007806 | 0.008027 | 0.005088 |
Bud Site | 0.024848 | 0.014092 | 0.017164 | 0.044405 | 0.008282 | 0.008086 | 0.034313 | 0.062117 | 0.015936 | 0.010340 | 0.031990 | 0.052140 |
Cell Periphery | 0.000507 | 0.000510 | 0.000365 | 0.000455 | 0.000269 | 0.000281 | 0.000244 | 0.002383 | 0.000379 | 0.000367 | 0.000261 | 0.001297 |
Cytoplasm | 0.093570 | 0.008501 | 0.002051 | 0.007615 | 0.008268 | 0.005250 | 0.010448 | 0.011910 | 0.047681 | 0.006470 | 0.009311 | 0.009491 |
Cytoplasmic Foci | 0.197011* | 0.066271 | 0.091235 | 0.047748 | 0.060872 | 0.046921 | 0.061890 | 0.039926 | 0.123773 | 0.054183 | 0.065865 | 0.044332 |
Eisosomes | 0.000312 | 0.000121 | 0.000097 | 0.000101 | 0.000104 | 0.000066 | 0.000089 | 0.000208 | 0.000200 | 0.000087 | 0.000090 | 0.000148 |
Endoplasmic Reticulum | 0.001855 | 0.000340 | 0.004163 | 0.001267 | 0.000411 | 0.000260 | 0.001210 | 0.001267 | 0.001078 | 0.000290 | 0.001610 | 0.001267 |
Endosome | 0.076705 | 0.090305 | 0.095013 | 0.079817 | 0.073931 | 0.088062 | 0.097928 | 0.052340 | 0.075213 | 0.088903 | 0.097533 | 0.067818 |
Golgi | 0.060681 | 0.129479* | 0.078345 | 0.099502 | 0.111930* | 0.116448* | 0.080747 | 0.075190 | 0.088251 | 0.121338* | 0.080422 | 0.088885 |
Lipid Particles | 0.028416 | 0.004538 | 0.006585 | 0.001968 | 0.005256 | 0.003838 | 0.001778 | 0.000763 | 0.015957 | 0.004101 | 0.002429 | 0.001442 |
Mitochondria | 0.327477* | 0.554902* | 0.502358* | 0.537942* | 0.654117* | 0.634622* | 0.484298* | 0.568024* | 0.503197* | 0.604705* | 0.486745* | 0.551078* |
Mitotic Spindle | 0.008063 | 0.012578 | 0.068931 | 0.055570 | 0.007475 | 0.016618 | 0.089620 | 0.028517 | 0.007746 | 0.015102 | 0.086817 | 0.043756 |
None | 0.010955 | 0.000614 | 0.000366 | 0.000326 | 0.000369 | 0.000519 | 0.000297 | 0.000287 | 0.005260 | 0.000554 | 0.000307 | 0.000309 |
Nuclear Periphery | 0.000545 | 0.000101 | 0.001862 | 0.000484 | 0.000100 | 0.000200 | 0.000318 | 0.000061 | 0.000306 | 0.000163 | 0.000527 | 0.000299 |
Nuclear Periphery Foci | 0.000615 | 0.000457 | 0.002060 | 0.000551 | 0.000237 | 0.000288 | 0.000480 | 0.000224 | 0.000412 | 0.000352 | 0.000694 | 0.000408 |
Nucleolus | 0.005574 | 0.001118 | 0.000699 | 0.000312 | 0.000466 | 0.001342 | 0.000727 | 0.000322 | 0.002826 | 0.001258 | 0.000723 | 0.000317 |
Nucleus | 0.026143 | 0.001379 | 0.001479 | 0.000852 | 0.000912 | 0.002089 | 0.002619 | 0.000649 | 0.012570 | 0.001822 | 0.002465 | 0.000763 |
Peroxisomes | 0.036921 | 0.083690 | 0.044019 | 0.038064 | 0.034369 | 0.034876 | 0.059589 | 0.101010* | 0.035548 | 0.053195 | 0.057480 | 0.065552 |
Vacuole | 0.037118 | 0.011326 | 0.036747 | 0.043151 | 0.013392 | 0.018356 | 0.041681 | 0.010952 | 0.024355 | 0.015718 | 0.041012 | 0.029090 |
Vacuole Periphery | 0.015751 | 0.008757 | 0.033433 | 0.026738 | 0.013042 | 0.013657 | 0.018120 | 0.007997 | 0.014293 | 0.011818 | 0.020195 | 0.018554 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.37 | -4.31 | -6.03 | -3.16 | -1.00 | 2.26 | -6.46 | -8.15 | -8.30 | -6.86 | -2.25 | -7.84 | -9.63 | -9.14 | -6.61 |
Bud Neck | 12.15 | 12.60 | 13.47 | 1.18 | 0.96 | -2.21 | -3.12 | 1.58 | 3.37 | 4.63 | 10.11 | 10.19 | 12.72 | 2.79 | 3.43 |
Bud Site | 4.52 | 2.01 | -5.23 | -8.37 | -6.02 | 0.16 | -12.24 | -11.73 | -11.74 | -5.48 | 4.49 | -8.08 | -12.43 | -14.66 | -6.06 |
Cell Periphery | -0.06 | 1.94 | 0.75 | 0.65 | -1.44 | -0.21 | 0.75 | -4.20 | -4.17 | -4.26 | 0.21 | 3.43 | -4.10 | -4.12 | -4.66 |
Cytoplasm | 18.88 | 21.01 | 19.22 | 0.23 | -6.21 | 2.76 | -2.00 | -2.64 | -5.03 | -0.74 | 18.12 | 15.98 | 16.31 | -3.57 | -0.16 |
Cytoplasmic Foci | 22.59 | 11.11 | 26.16 | 5.04 | 5.08 | 4.72 | -0.34 | 6.09 | 2.35 | 6.57 | 21.11 | 15.69 | 23.09 | 4.27 | 7.51 |
Eisosomes | 3.09 | 3.47 | 3.42 | 2.57 | -0.44 | 5.82 | 2.03 | -7.32 | -10.88 | -8.91 | 3.95 | 3.79 | 1.77 | -8.78 | -8.01 |
Endoplasmic Reticulum | 5.16 | -0.84 | 1.69 | -5.59 | 1.05 | 2.56 | -4.53 | -3.41 | -4.00 | -0.53 | 5.67 | -1.32 | -1.29 | -6.69 | 0.68 |
Endosome | -2.77 | -2.44 | -0.63 | 2.01 | 1.98 | -3.31 | -5.35 | 4.86 | 8.04 | 9.83 | -4.27 | -6.01 | 2.21 | 6.11 | 7.58 |
Golgi | -12.44 | -2.33 | -6.50 | 4.46 | -2.33 | -0.84 | 6.34 | 6.20 | 7.16 | 0.95 | -8.57 | 2.12 | -0.07 | 7.29 | -1.89 |
Lipid Particles | 10.61 | 7.12 | 12.00 | 2.70 | 1.96 | 1.44 | 4.04 | 5.18 | 5.21 | 2.77 | 10.10 | 11.75 | 12.59 | 4.72 | 1.92 |
Mitochondria | -17.39 | -7.21 | -14.57 | 1.37 | -1.23 | 1.83 | 14.91 | 5.72 | 4.52 | -5.54 | -11.65 | 1.77 | -4.29 | 5.38 | -5.48 |
Mitotic Spindle | -3.11 | -7.05 | -11.14 | -9.96 | 1.32 | -5.50 | -19.63* | -7.57 | -4.01 | 12.61 | -6.33 | -20.95 | -13.28 | -10.25 | 9.35 |
None | 6.49 | 6.72 | 6.74 | 1.68 | 0.69 | -1.57 | 0.84 | 0.93 | 3.23 | 0.24 | 6.34 | 6.73 | 6.73 | 3.02 | -0.03 |
Nuclear Periphery | 6.02 | -2.05 | 0.57 | -3.98 | 2.14 | -1.36 | -5.43 | 1.74 | 1.96 | 6.99 | 2.58 | -2.26 | 0.13 | -1.99 | 2.17 |
Nuclear Periphery Foci | 0.52 | -3.35 | 0.51 | -0.28 | 3.54 | -0.43 | -2.21 | 0.07 | 0.59 | 2.71 | 0.43 | -2.75 | 0.03 | -0.44 | 3.05 |
Nucleolus | 5.27 | 6.89 | 7.72 | 1.74 | 1.44 | -2.04 | -1.04 | 0.55 | 2.17 | 1.30 | 3.64 | 5.70 | 7.26 | 2.92 | 1.93 |
Nucleus | 13.33 | 12.58 | 13.82 | 1.04 | 0.66 | -2.19 | -3.44 | 0.62 | 2.45 | 3.63 | 11.45 | 10.13 | 13.22 | 2.61 | 3.69 |
Peroxisomes | -8.81 | -1.12 | -0.36 | 7.75 | 0.83 | -0.13 | -6.29 | -8.85 | -8.88 | -5.26 | -6.22 | -6.61 | -7.46 | -2.86 | -1.71 |
Vacuole | 11.15 | 0.05 | -2.14 | -10.45 | -1.57 | -2.98 | -12.12 | 1.30 | 3.92 | 12.46 | 6.00 | -7.80 | -2.73 | -7.09 | 4.40 |
Vacuole Periphery | 5.15 | -4.34 | -5.04 | -8.71 | 1.57 | -0.57 | -3.79 | 4.35 | 4.88 | 7.30 | 2.81 | -4.70 | -3.20 | -5.18 | 1.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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