Standard name | |
---|---|
Human Ortholog | |
Description | 1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 13 | 25 | 22 | 19 | 32 | 32 | 39 | – | 19 | 21 | 28 | 17 | 22 | 14 | 5 | 14 | 9 | 2 | 5 | 8 | 3 | 10 | 16 |
Bud | 0 | 1 | 4 | 0 | 1 | 4 | 5 | 11 | – | 6 | 7 | 7 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 11 | 5 | 4 | 8 | 6 | 3 | 0 | – | 1 | 1 | 2 | 9 | 33 | 19 | 3 | 3 | 5 | 0 | 0 | 2 | 1 | 2 | 3 |
Cytoplasm | 1 | 12 | 8 | 6 | 11 | 11 | 9 | 11 | – | 13 | 9 | 5 | 21 | 13 | 18 | 17 | 12 | 14 | 1 | 3 | 0 | 3 | 5 | 3 |
Endoplasmic Reticulum | 9 | 241 | 246 | 129 | 205 | 240 | 215 | 144 | – | 43 | 53 | 71 | 241 | 242 | 231 | 5 | 5 | 4 | 42 | 244 | 276 | 203 | 247 | 305 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | – | 3 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 4 | 3 |
Golgi | 5 | 21 | 60 | 54 | 29 | 29 | 60 | 68 | – | 50 | 26 | 51 | 97 | 73 | 73 | 169 | 197 | 153 | 13 | 2 | 3 | 11 | 8 | 16 |
Mitochondria | 8 | 0 | 8 | 8 | 7 | 44 | 92 | 113 | – | 134 | 207 | 281 | 0 | 0 | 0 | 22 | 39 | 18 | 0 | 0 | 1 | 3 | 2 | 8 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 3 | 4 | 4 | 11 | 3 | – | 9 | 11 | 11 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 3 | 2 | 3 | 0 | 0 | 5 | 15 | 34 | – | 40 | 55 | 86 | 4 | 4 | 3 | 7 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 2 | 6 | 2 | 5 | 11 | 11 | 23 | 47 | – | 47 | 37 | 39 | 5 | 8 | 12 | 1 | 2 | 11 | 0 | 2 | 0 | 6 | 4 | 6 |
Unique Cell Count | 21 | 263 | 274 | 161 | 243 | 291 | 341 | 352 | 274 | 325 | 437 | 302 | 289 | 294 | 198 | 220 | 183 | 70 | 264 | 295 | 243 | 293 | 374 | |
Labelled Cell Count | 31 | 307 | 362 | 232 | 295 | 387 | 467 | 472 | 366 | 427 | 588 | 398 | 397 | 371 | 229 | 278 | 219 | 70 | 264 | 295 | 243 | 293 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.8 | 35.4 | 23.8 | 21.8 | 20.8 | 18.1 | 17.8 | 18.4 | – | 16.4 | 17.0 | 17.4 | 28.9 | 27.3 | 27.6 | 39.2 | 41.2 | 42.2 | 29.1 | 30.2 | 30.2 |
Std Deviation (1e-4) | 10.6 | 5.9 | 3.9 | 3.4 | 3.6 | 3.2 | 3.1 | 3.1 | – | 3.6 | 3.3 | 3.9 | 6.2 | 5.2 | 5.4 | 10.7 | 10.0 | 10.8 | 6.1 | 6.3 | 6.3 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.19 | -0.39 | -0.42 | -0.37 | – | -0.54 | -0.48 | -0.45 | 0.28 | 0.2 | 0.21 | 0.72 | 0.79 | 0.83 | 0.29 | 0.34 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | -0.5 | 0.7 | 0.1 | 0.8 | – | -0.9 | -1.2 | -1.3 | -1.6 | -0.6 | -2.1 | -2.9 | -1.1 | -1.7 |
Bud | 0 | 0 | 0 | 0 | 1.4 | – | 0.6 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.1 | 0.2 | 0 | 0 | – | 0 | 0 | 0 | 0.9 | 4.5 | 2.7 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 1.0 | 0.6 | -0.2 | 0.1 | – | 1.1 | -0.1 | -1.7 | 2.2 | 1.0 | 1.8 | 2.7 | 1.4 | 2.3 |
Endoplasmic Reticulum | -2.8 | -1.8 | -2.5 | -7.6 | -12.5 | – | -17.4 | -17.9 | -19.2 | -3.3 | -2.1 | -3.6 | -18.7 | -19.3 | -18.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.7 | -3.0 | -3.9 | -1.3 | -0.8 | – | -1.1 | -4.8 | -3.7 | 2.8 | 0.9 | 0.8 | 13.6 | 15.0 | 13.0 |
Mitochondria | 1.1 | 0 | 5.0 | 8.0 | 9.2 | – | 12.3 | 15.4 | 16.2 | 0 | 0 | 0 | 3.6 | 5.6 | 3.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.5 | 0 | – | 2.6 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.4 | 4.5 | – | 5.9 | 6.5 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.8 | 2.4 | 3.8 | 5.8 | – | 6.7 | 5.3 | 4.6 | 0 | 0 | 2.6 | 0 | 0 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.2031 | 27.4104 | 23.6389 | 23.4212 | 22.7053 | 24.4401 | 27.7275 | 29.8772 | 27.9439 | 26.4931 | 25.4833 | 28.0729 | 26.4722 | 27.4494 | 26.8512 | 26.2375 | 23.2648 | 26.1766 |
Actin | 0.0024 | 0.0002 | 0.0008 | 0.0123 | 0.0141 | 0.0032 | 0.0069 | 0.0003 | 0.0006 | 0.0127 | 0.0058 | 0.0011 | 0.0011 | 0.0005 | 0.0012 | 0.0002 | 0.0009 | 0.0004 |
Bud | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0007 | 0.0009 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Periphery | 0.0002 | 0 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0006 | 0 | 0 | 0.0006 | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0151 | 0.0228 | 0.017 | 0.011 | 0.0077 | 0.0066 | 0.0117 | 0.0116 | 0.0109 | 0.0077 | 0.0035 | 0.0045 | 0.0144 | 0.0206 | 0.0171 | 0.0122 | 0.0053 | 0.0055 |
Cytoplasm | 0.0059 | 0.0078 | 0.0021 | 0.0018 | 0.0017 | 0.0019 | 0.0042 | 0.001 | 0.0016 | 0.0006 | 0.0019 | 0.0006 | 0.004 | 0.0144 | 0.0011 | 0.0008 | 0.0076 | 0.0011 |
Cytoplasmic Foci | 0.0069 | 0.0014 | 0.0018 | 0.005 | 0.0033 | 0.0025 | 0.0052 | 0.0016 | 0.002 | 0.0061 | 0.0055 | 0.0007 | 0.0038 | 0.0022 | 0.0019 | 0.0011 | 0.0122 | 0.0011 |
Eisosomes | 0.0007 | 0.0021 | 0.0004 | 0.0026 | 0.0012 | 0.0003 | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0037 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.4791 | 0.5815 | 0.6855 | 0.6175 | 0.7123 | 0.6877 | 0.614 | 0.7388 | 0.8038 | 0.7129 | 0.7079 | 0.798 | 0.5535 | 0.6218 | 0.5858 | 0.7412 | 0.7293 | 0.7505 |
Endosome | 0.0225 | 0.0037 | 0.0103 | 0.0215 | 0.0104 | 0.0075 | 0.0136 | 0.0051 | 0.007 | 0.0041 | 0.0095 | 0.0053 | 0.0142 | 0.006 | 0.0071 | 0.0051 | 0.0155 | 0.0059 |
Golgi | 0.025 | 0.0095 | 0.0203 | 0.0392 | 0.0236 | 0.0206 | 0.0204 | 0.0163 | 0.0187 | 0.0143 | 0.0465 | 0.0153 | 0.0254 | 0.0069 | 0.0346 | 0.0279 | 0.0284 | 0.0281 |
Lipid Particles | 0.3292 | 0.3283 | 0.2347 | 0.2408 | 0.1894 | 0.2255 | 0.2533 | 0.2148 | 0.1318 | 0.2028 | 0.1685 | 0.1489 | 0.3196 | 0.2984 | 0.3376 | 0.1901 | 0.1715 | 0.181 |
Mitochondria | 0.0065 | 0.0001 | 0.0005 | 0.0174 | 0.0061 | 0.001 | 0.0041 | 0.0004 | 0.0086 | 0.0015 | 0.0014 | 0.0006 | 0.0014 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0002 |
None | 0.0008 | 0.0002 | 0.0001 | 0.0016 | 0.0008 | 0.0003 | 0.0038 | 0.0001 | 0.0001 | 0.0001 | 0.0029 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0082 | 0.0001 |
Nuclear Periphery | 0.0792 | 0.0255 | 0.0147 | 0.0075 | 0.0213 | 0.0326 | 0.0395 | 0.004 | 0.0077 | 0.0134 | 0.0181 | 0.0189 | 0.0452 | 0.0166 | 0.0061 | 0.0117 | 0.0141 | 0.0205 |
Nucleolus | 0.0014 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0008 | 0 | 0 | 0 | 0.0021 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0034 | 0.0022 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0.0025 | 0 | 0.0001 | 0 | 0.0111 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Peroxisomes | 0.0004 | 0 | 0 | 0.0004 | 0.0004 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0178 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Punctate Nuclear | 0.0023 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0021 | 0.0016 | 0 | 0.0001 | 0.0002 | 0.0095 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0015 | 0.0001 |
Vacuole | 0.0129 | 0.0126 | 0.0085 | 0.0135 | 0.0037 | 0.0045 | 0.0105 | 0.0047 | 0.0045 | 0.004 | 0.0026 | 0.0033 | 0.0113 | 0.0099 | 0.0055 | 0.0038 | 0.0032 | 0.0034 |
Vacuole Periphery | 0.0052 | 0.0016 | 0.002 | 0.0034 | 0.0027 | 0.002 | 0.0048 | 0.0007 | 0.0017 | 0.001 | 0.0022 | 0.0019 | 0.0034 | 0.0009 | 0.0008 | 0.0011 | 0.0013 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 74.4072 | 69.2194 | 47.351 | 55.3265 | 72.9424 | 64.0878 | 70.5344 | 74.9148 | 72.0455 | 82.269 |
Translational Efficiency | 1.2205 | 1.0525 | 1.2332 | 1.1156 | 0.9118 | 1.3561 | 1.0798 | 0.9977 | 0.9333 | 0.8784 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1768 | 1507 | 332 | 1857 | 1964 | 1305 | 2612 | 1313 | 3732 | 2812 | 2944 | 3170 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1853.25 | 2295.47 | 2708.85 | 3105.73 | 1870.32 | 1891.13 | 2725.55 | 2935.78 | 1862.23 | 2107.82 | 2723.67 | 3035.34 |
Standard Deviation | 354.20 | 422.99 | 362.84 | 591.37 | 472.11 | 343.26 | 417.73 | 623.29 | 420.48 | 437.30 | 411.94 | 610.56 |
Intensity Change Log 2 | — | 0.308732 | 0.547623 | 0.744875 | — | 0.015963 | 0.543262 | 0.650459 | — | 0.169093 | 0.545434 | 0.698223 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000408 | 0.002499 | 0.000197 | 0.006499 | 0.003203 | 0.001954 | 0.003091 | 0.010025 | 0.001879 | 0.002246 | 0.002765 | 0.007960 |
Bud Neck | 0.000225 | 0.001416 | 0.003647 | 0.005214 | 0.001143 | 0.000682 | 0.001610 | 0.004949 | 0.000708 | 0.001076 | 0.001840 | 0.005104 |
Bud Site | 0.001422 | 0.002256 | 0.000610 | 0.005919 | 0.004322 | 0.001838 | 0.000899 | 0.006345 | 0.002948 | 0.002062 | 0.000866 | 0.006095 |
Cell Periphery | 0.003142 | 0.002510 | 0.006590 | 0.004656 | 0.002112 | 0.002084 | 0.002512 | 0.007384 | 0.002600 | 0.002312 | 0.002972 | 0.005786 |
Cytoplasm | 0.001448 | 0.000479 | 0.000120 | 0.000717 | 0.001777 | 0.001116 | 0.000263 | 0.000773 | 0.001621 | 0.000775 | 0.000247 | 0.000740 |
Cytoplasmic Foci | 0.002661 | 0.001078 | 0.000075 | 0.004169 | 0.003843 | 0.001801 | 0.001343 | 0.003118 | 0.003283 | 0.001413 | 0.001200 | 0.003734 |
Eisosomes | 0.000095 | 0.000364 | 0.000237 | 0.000863 | 0.000477 | 0.000166 | 0.000982 | 0.001457 | 0.000296 | 0.000272 | 0.000898 | 0.001109 |
Endoplasmic Reticulum | 0.937597* | 0.795092* | 0.894678* | 0.371870* | 0.745693* | 0.907372* | 0.567231* | 0.442436* | 0.836606* | 0.847199* | 0.604158* | 0.401098* |
Endosome | 0.014479 | 0.038288 | 0.012357 | 0.062534 | 0.037955 | 0.020688 | 0.047387 | 0.046710 | 0.026833 | 0.030120 | 0.043437 | 0.055980 |
Golgi | 0.010319 | 0.033656 | 0.016101 | 0.081711 | 0.042978 | 0.016893 | 0.071997 | 0.090485 | 0.027506 | 0.025876 | 0.065694 | 0.085345 |
Lipid Particles | 0.013539 | 0.110336* | 0.028039 | 0.404525* | 0.109554* | 0.032315 | 0.283539* | 0.338026* | 0.064068 | 0.074128 | 0.254726* | 0.376981* |
Mitochondria | 0.000584 | 0.000285 | 0.000155 | 0.001583 | 0.001040 | 0.000401 | 0.000307 | 0.001982 | 0.000824 | 0.000339 | 0.000289 | 0.001749 |
Mitotic Spindle | 0.000092 | 0.000113 | 0.000050 | 0.002989 | 0.000368 | 0.001448 | 0.000060 | 0.001824 | 0.000237 | 0.000732 | 0.000058 | 0.002506 |
None | 0.001155 | 0.001592 | 0.000066 | 0.000775 | 0.004980 | 0.000924 | 0.001789 | 0.000417 | 0.003168 | 0.001282 | 0.001594 | 0.000627 |
Nuclear Periphery | 0.000694 | 0.001119 | 0.007031 | 0.002785 | 0.001906 | 0.000698 | 0.001618 | 0.002235 | 0.001332 | 0.000924 | 0.002229 | 0.002557 |
Nuclear Periphery Foci | 0.004471 | 0.003382 | 0.008739 | 0.011849 | 0.021424 | 0.004006 | 0.003651 | 0.006604 | 0.013393 | 0.003672 | 0.004225 | 0.009677 |
Nucleolus | 0.000008 | 0.000007 | 0.000011 | 0.000033 | 0.000214 | 0.000010 | 0.000009 | 0.000299 | 0.000116 | 0.000008 | 0.000010 | 0.000143 |
Nucleus | 0.000078 | 0.000040 | 0.000050 | 0.000124 | 0.001009 | 0.000213 | 0.000032 | 0.000315 | 0.000568 | 0.000120 | 0.000034 | 0.000203 |
Peroxisomes | 0.000301 | 0.000126 | 0.000003 | 0.000106 | 0.000192 | 0.000057 | 0.000205 | 0.000165 | 0.000243 | 0.000094 | 0.000182 | 0.000131 |
Vacuole | 0.005006 | 0.004197 | 0.017577 | 0.024967 | 0.011609 | 0.003645 | 0.008779 | 0.027471 | 0.008481 | 0.003941 | 0.009771 | 0.026004 |
Vacuole Periphery | 0.002275 | 0.001168 | 0.003668 | 0.006111 | 0.004201 | 0.001688 | 0.002695 | 0.006980 | 0.003289 | 0.001409 | 0.002805 | 0.006471 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.08 | 1.16 | -5.86 | -3.33 | -6.03 | 1.26 | 0.13 | -3.80 | -4.37 | -3.97 | -0.62 | -1.68 | -6.22 | -5.49 | -5.18 |
Bud Neck | -3.18 | -2.55 | -8.88 | -5.73 | -0.91 | 2.44 | -1.98 | -6.78 | -7.57 | -5.82 | -1.69 | -4.65 | -10.87 | -9.02 | -7.13 |
Bud Site | -1.13 | 1.24 | -5.23 | -3.91 | -6.36 | 2.83 | 4.86 | -2.12 | -4.87 | -6.86 | 1.43 | 4.76 | -4.82 | -6.04 | -9.30 |
Cell Periphery | 0.86 | -2.45 | -3.00 | -3.60 | 1.33 | 0.13 | -1.87 | -8.97 | -8.63 | -8.33 | 0.72 | -1.24 | -8.71 | -8.22 | -8.12 |
Cytoplasm | 2.40 | 3.39 | 1.52 | -1.40 | -3.36 | 1.03 | 5.26 | 2.46 | 0.74 | -2.27 | 2.45 | 5.78 | 2.88 | 0.09 | -3.63 |
Cytoplasmic Foci | 2.34 | 4.42 | -2.24 | -6.27 | -11.02 | 3.09 | 4.95 | 1.10 | -2.11 | -3.88 | 4.01 | 5.24 | -1.00 | -5.94 | -8.19 |
Eisosomes | -3.10 | -2.89 | -7.69 | -3.83 | -5.75 | 2.55 | -2.28 | -3.90 | -5.86 | -1.62 | 0.30 | -3.40 | -6.47 | -7.13 | -1.04 |
Endoplasmic Reticulum | 18.11 | 4.03 | 66.30* | 40.14* | 38.27* | -17.54* | 17.43 | 24.90 | 42.25* | 10.66 | -1.61 | 29.21 | 57.29 | 58.16* | 22.82 |
Endosome | -13.92 | 1.16 | -23.02 | -9.79 | -20.57 | 8.90 | -4.55 | -3.60 | -11.76 | 0.25 | -2.42 | -10.81 | -17.57 | -15.15 | -6.78 |
Golgi | -11.84 | -2.78 | -24.26 | -14.74 | -19.75* | 10.62 | -9.57 | -11.78 | -20.12 | -4.55 | 0.99 | -16.49 | -23.25 | -24.53 | -6.71 |
Lipid Particles | -17.77* | -2.90 | -51.68* | -32.01* | -40.85* | 13.81 | -22.55 | -23.21 | -33.79* | -5.14 | -2.42 | -30.71 | -49.81 | -46.38* | -15.23 |
Mitochondria | 1.94 | 2.85 | -2.56 | -3.52 | -3.88 | 1.76 | 2.13 | -1.52 | -2.82 | -3.04 | 2.41 | 2.73 | -2.60 | -4.44 | -4.64 |
Mitotic Spindle | -0.33 | 1.20 | -3.52 | -3.48 | -3.56 | -1.53 | 2.78 | -1.92 | -0.38 | -2.33 | -1.50 | 2.95 | -3.93 | -2.70 | -4.26 |
None | -0.89 | 4.01 | 0.96 | 1.66 | -3.82 | 3.92 | 3.10 | 4.49 | 1.31 | 3.65 | 3.16 | 2.64 | 4.49 | 2.04 | 2.96 |
Nuclear Periphery | -3.07 | -5.48 | -8.43 | -6.25 | 3.62 | 3.19 | 0.69 | -0.96 | -7.07 | -2.87 | 1.93 | -3.66 | -5.01 | -9.13 | -1.42 |
Nuclear Periphery Foci | 1.10 | -2.65 | -6.10 | -7.93 | -1.58 | 6.67 | 7.23 | 5.76 | -2.35 | -3.88 | 6.75 | 6.54 | 2.39 | -7.50 | -7.50 |
Nucleolus | 0.68 | -0.67 | -2.72 | -2.84 | -2.37 | 2.92 | 2.92 | -0.37 | -1.26 | -1.26 | 2.94 | 2.90 | -0.29 | -1.43 | -1.41 |
Nucleus | 2.66 | 1.47 | -2.22 | -4.00 | -3.23 | 1.46 | 1.89 | 1.27 | -0.42 | -1.66 | 1.58 | 1.96 | 1.25 | -0.90 | -2.47 |
Peroxisomes | 1.46 | 2.61 | 1.69 | 0.47 | -6.45 | 1.71 | -0.14 | 0.24 | -1.16 | 0.40 | 2.12 | 0.79 | 1.46 | -0.83 | 0.93 |
Vacuole | 1.12 | -5.22 | -11.84 | -12.43 | -2.37 | 5.85 | 2.00 | -6.90 | -11.58 | -8.97 | 5.63 | -1.36 | -12.44 | -17.01 | -11.57 |
Vacuole Periphery | 2.92 | -1.57 | -5.89 | -8.84 | -2.42 | 4.06 | 3.25 | -4.09 | -7.44 | -7.35 | 5.27 | 1.42 | -6.67 | -11.27 | -8.44 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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