Standard name | |
---|---|
Human Ortholog | |
Description | Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 2 | 2 | 7 | 8 | 0 | 1 | 8 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 6 | 9 | 4 | 2 | 9 | 4 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 5 | 15 | 9 | 4 | 20 | 11 | 7 | 9 | 12 | 11 | 12 | 23 | 23 | 26 | 18 | 11 | 0 | 0 | 0 | 3 | 6 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 9 | 3 | 0 | 0 | 0 | 2 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 10 | 16 | 2 | 1 | 1 | 1 | 0 | 3 | 5 | 7 | 10 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 0 | 1 | 0 | 2 | 0 | 2 | 3 | 12 | 18 |
Mitochondria | 32 | 13 | 10 | 50 | 73 | 172 | 375 | 282 | 252 | 385 | 412 | 455 | 19 | 15 | 28 | 5 | 8 | 4 | 11 | 1 | 29 | 25 | 31 | 43 |
Nucleus | 144 | 93 | 197 | 272 | 242 | 344 | 442 | 414 | 276 | 313 | 276 | 309 | 242 | 363 | 381 | 258 | 234 | 209 | 120 | 81 | 110 | 136 | 127 | 104 |
Nuclear Periphery | 2 | 0 | 2 | 1 | 5 | 13 | 23 | 35 | 14 | 15 | 18 | 19 | 1 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 4 |
Nucleolus | 2 | 2 | 1 | 1 | 1 | 10 | 19 | 18 | 8 | 10 | 19 | 11 | 1 | 0 | 1 | 3 | 0 | 4 | 0 | 0 | 1 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 4 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 0 |
Vac/Vac Membrane | 38 | 28 | 48 | 29 | 56 | 65 | 73 | 99 | 61 | 63 | 66 | 110 | 82 | 145 | 123 | 21 | 27 | 20 | 73 | 43 | 140 | 172 | 279 | 333 |
Unique Cell Count | 194 | 125 | 251 | 342 | 349 | 504 | 747 | 706 | 484 | 604 | 584 | 693 | 348 | 537 | 564 | 311 | 282 | 240 | 213 | 131 | 293 | 358 | 478 | 536 |
Labelled Cell Count | 219 | 137 | 263 | 369 | 392 | 616 | 968 | 875 | 621 | 806 | 815 | 934 | 366 | 572 | 600 | 327 | 300 | 252 | 213 | 131 | 293 | 358 | 478 | 536 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 8.8 | 8.4 | 7.8 | 7.1 | 5.9 | 5.3 | 5.5 | 5.0 | 4.7 | 4.5 | 4.6 | 9.9 | 9.3 | 9.0 | 12.1 | 12.1 | 11.2 | 10.9 | 11.2 | 11.5 |
Std Deviation (1e-4) | 1.5 | 2.1 | 1.7 | 1.9 | 1.8 | 1.4 | 1.3 | 1.3 | 1.2 | 1.1 | 1.3 | 1.1 | 6.4 | 1.8 | 2.0 | 3.4 | 3.6 | 3.2 | 2.2 | 2.3 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.25 | -0.51 | -0.68 | -0.62 | -0.76 | -0.83 | -0.91 | -0.88 | 0.23 | 0.14 | 0.09 | 0.52 | 0.52 | 0.41 | 0.36 | 0.41 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 0.5 | 0 | 0.6 | 0 | 0 | 0 | 0.1 | 0 | 1.1 | 1.6 | 1.5 | 3.3 | 2.5 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 5.9 | 9.1 | 13.0 | 10.6 | 12.9 | 0 | 0 | 0 | 0.8 | -0.9 | 0.6 | -1.7 | -0.7 | -1.5 |
Nucleus | 0.3 | -2.5 | -2.9 | -5.5 | -5.6 | -5.8 | -7.2 | -8.3 | -9.2 | -2.4 | -3.1 | -3.2 | 1.3 | 1.3 | 2.5 |
Nuclear Periphery | 0 | 0 | 0 | 2.0 | 2.9 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.8 | -1.0 | -2.3 | -3.9 | -1.9 | -2.4 | -3.4 | -3.0 | -1.2 | 1.3 | 2.4 | 0.9 | -4.4 | -3.2 | -3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1019 | 6.5086 | 5.9579 | 5.9946 | 4.905 | 5.9779 | 3.6082 | 4.7401 | 4.1081 | 3.8326 | 3.1493 | 3.8243 | 2.4595 | 3.3271 | 2.67 | 2.0956 | 1.6465 | 2.5604 |
Actin | 0.0105 | 0.0004 | 0.0146 | 0.0068 | 0.0023 | 0.0008 | 0.0112 | 0.0004 | 0.0064 | 0.0144 | 0.0148 | 0.0025 | 0.0081 | 0 | 0.0156 | 0.0108 | 0.0022 | 0 |
Bud | 0.0005 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 |
Bud Neck | 0.0018 | 0.0002 | 0.0004 | 0.0027 | 0.001 | 0.0023 | 0.0017 | 0.0001 | 0.0007 | 0.0003 | 0.0006 | 0.003 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0007 |
Bud Periphery | 0.0012 | 0.0005 | 0.0002 | 0.0022 | 0.0004 | 0.0009 | 0.0019 | 0.0001 | 0.0008 | 0.0005 | 0.0029 | 0.0012 | 0.0009 | 0.0002 | 0.0003 | 0.0008 | 0.001 | 0.0003 |
Bud Site | 0.0013 | 0.0005 | 0.0005 | 0.0008 | 0.0003 | 0.0001 | 0.0037 | 0.0001 | 0.0007 | 0.0007 | 0.0007 | 0.0005 | 0.001 | 0.0001 | 0.0007 | 0.0011 | 0.0004 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0009 | 0 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0022 | 0.0011 | 0.0004 | 0.0015 | 0.0105 | 0.0012 | 0.0044 | 0.0016 | 0.0019 | 0.0097 | 0.0005 | 0.0009 | 0.0075 | 0.0008 | 0.001 | 0.0009 | 0.0009 | 0.0014 |
Cytoplasmic Foci | 0.0021 | 0.0015 | 0.0004 | 0.001 | 0.004 | 0.0009 | 0.0146 | 0 | 0.0059 | 0.0086 | 0.0007 | 0.001 | 0.01 | 0 | 0.0066 | 0.0117 | 0.0003 | 0.0001 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.005 | 0.0005 | 0.0009 | 0.0024 | 0.0067 | 0.0005 | 0.0036 | 0.0004 | 0.001 | 0.0032 | 0.0008 | 0.002 | 0.0033 | 0.0002 | 0.0004 | 0.0016 | 0.0024 | 0.0002 |
Endosome | 0.0128 | 0.0028 | 0.0015 | 0.0073 | 0.0046 | 0.001 | 0.0199 | 0.0003 | 0.013 | 0.0221 | 0.0047 | 0.0058 | 0.0333 | 0.0001 | 0.0061 | 0.0317 | 0.008 | 0.0021 |
Golgi | 0.0035 | 0.0004 | 0.0011 | 0.0023 | 0.0013 | 0.0002 | 0.0045 | 0 | 0.0053 | 0.0092 | 0.0072 | 0.0027 | 0.0034 | 0 | 0.003 | 0.0173 | 0.0008 | 0 |
Lipid Particles | 0.0054 | 0.0043 | 0.001 | 0.011 | 0.0135 | 0.0002 | 0.0091 | 0 | 0.0051 | 0.0104 | 0.0034 | 0.0108 | 0.0098 | 0 | 0.0084 | 0.0054 | 0.0007 | 0 |
Mitochondria | 0.0205 | 0.0084 | 0.0107 | 0.0508 | 0.0213 | 0.0038 | 0.021 | 0.0072 | 0.0236 | 0.0364 | 0.0802 | 0.0119 | 0.0189 | 0.0016 | 0.0072 | 0.0538 | 0.0459 | 0.0012 |
None | 0.0014 | 0.0002 | 0.0003 | 0.0018 | 0.0007 | 0.0002 | 0.0052 | 0.0001 | 0.0005 | 0.0077 | 0.0005 | 0.0003 | 0.0012 | 0 | 0.0005 | 0.0003 | 0.0006 | 0 |
Nuclear Periphery | 0.0275 | 0.0215 | 0.0247 | 0.0233 | 0.0312 | 0.0151 | 0.0353 | 0.0155 | 0.0197 | 0.0066 | 0.0107 | 0.0173 | 0.0167 | 0.0135 | 0.0128 | 0.0152 | 0.0517 | 0.0022 |
Nucleolus | 0.0119 | 0.0069 | 0.0054 | 0.0162 | 0.023 | 0.0195 | 0.008 | 0.0055 | 0.007 | 0.004 | 0.0164 | 0.0119 | 0.0073 | 0.0049 | 0.0083 | 0.0073 | 0.0267 | 0.0139 |
Nucleus | 0.8723 | 0.9435 | 0.934 | 0.8522 | 0.867 | 0.9465 | 0.8222 | 0.9666 | 0.894 | 0.8566 | 0.8426 | 0.9155 | 0.8553 | 0.9758 | 0.9111 | 0.807 | 0.8467 | 0.9691 |
Peroxisomes | 0.003 | 0.0002 | 0.0007 | 0.001 | 0.0018 | 0.0001 | 0.011 | 0 | 0.0015 | 0.0024 | 0.0012 | 0.001 | 0.0008 | 0 | 0.0088 | 0.0237 | 0.0002 | 0 |
Punctate Nuclear | 0.0057 | 0.0016 | 0.0005 | 0.0055 | 0.0053 | 0.0027 | 0.0057 | 0.0002 | 0.0025 | 0.0026 | 0.0004 | 0.0027 | 0.0107 | 0.0001 | 0.002 | 0.0014 | 0.0022 | 0.0002 |
Vacuole | 0.0054 | 0.0016 | 0.0009 | 0.0038 | 0.0022 | 0.0016 | 0.008 | 0.0007 | 0.0043 | 0.0014 | 0.0034 | 0.0045 | 0.0062 | 0.0012 | 0.0034 | 0.0024 | 0.0027 | 0.0044 |
Vacuole Periphery | 0.0052 | 0.0035 | 0.0014 | 0.0059 | 0.0025 | 0.0021 | 0.0073 | 0.0011 | 0.0056 | 0.0024 | 0.0066 | 0.0037 | 0.0042 | 0.0011 | 0.0033 | 0.007 | 0.0057 | 0.0036 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.7218 | 52.0943 | 40.5574 | 32.0996 | 27.2498 | 30.5536 | 33.2441 | 35.259 | 29.2045 | 31.2867 |
Translational Efficiency | 0.8526 | 0.7466 | 0.8644 | 0.9014 | 1.1285 | 0.9632 | 0.8278 | 0.8444 | 0.8942 | 0.8499 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1564 | 109 | 262 | 649 | 1756 | 1690 | 1258 | 1283 | 3320 | 1799 | 1520 | 1932 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 944.00 | 1144.60 | 1222.73 | 1188.11 | 906.79 | 1001.16 | 1117.33 | 1152.77 | 924.32 | 1009.85 | 1135.50 | 1164.64 |
Standard Deviation | 136.15 | 204.49 | 174.48 | 184.37 | 143.30 | 163.82 | 163.82 | 204.04 | 141.20 | 170.05 | 170.42 | 198.35 |
Intensity Change Log 2 | — | 0.277985 | 0.373247 | 0.331810 | — | 0.142832 | 0.301215 | 0.346264 | — | 0.213348 | 0.338404 | 0.338910 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003706 | 0.006811 | 0.000964 | 0.021662 | 0.003368 | 0.003770 | 0.000892 | 0.023047 | 0.003528 | 0.003955 | 0.000904 | 0.022582 |
Bud Neck | 0.027942 | 0.048137 | 0.011944 | 0.130790 | 0.035962 | 0.047124 | 0.011741 | 0.141940 | 0.032184 | 0.047186 | 0.011776 | 0.138194 |
Bud Site | 0.007965 | 0.013482 | 0.001070 | 0.049648 | 0.010528 | 0.010364 | 0.001326 | 0.079053 | 0.009321 | 0.010553 | 0.001282 | 0.069175 |
Cell Periphery | 0.000652 | 0.000299 | 0.000059 | 0.000595 | 0.000780 | 0.000885 | 0.000196 | 0.001016 | 0.000720 | 0.000850 | 0.000172 | 0.000875 |
Cytoplasm | 0.000813 | 0.009671 | 0.000230 | 0.005057 | 0.002167 | 0.001241 | 0.001353 | 0.008137 | 0.001530 | 0.001752 | 0.001160 | 0.007102 |
Cytoplasmic Foci | 0.000999 | 0.001225 | 0.000034 | 0.002057 | 0.000959 | 0.002732 | 0.000059 | 0.003934 | 0.000978 | 0.002641 | 0.000055 | 0.003303 |
Eisosomes | 0.000121 | 0.000230 | 0.000056 | 0.000185 | 0.000104 | 0.000145 | 0.000047 | 0.000215 | 0.000112 | 0.000151 | 0.000048 | 0.000205 |
Endoplasmic Reticulum | 0.065452 | 0.040384 | 0.005749 | 0.050984 | 0.063435 | 0.056654 | 0.008953 | 0.058706 | 0.064385 | 0.055668 | 0.008400 | 0.056112 |
Endosome | 0.001344 | 0.001085 | 0.000107 | 0.004253 | 0.002024 | 0.003852 | 0.000219 | 0.006045 | 0.001704 | 0.003685 | 0.000200 | 0.005443 |
Golgi | 0.000694 | 0.000547 | 0.000041 | 0.001756 | 0.000668 | 0.001411 | 0.000048 | 0.005178 | 0.000681 | 0.001359 | 0.000047 | 0.004029 |
Lipid Particles | 0.001832 | 0.001516 | 0.000100 | 0.002619 | 0.001896 | 0.002369 | 0.000095 | 0.002720 | 0.001866 | 0.002317 | 0.000096 | 0.002686 |
Mitochondria | 0.035969 | 0.020089 | 0.001468 | 0.080997 | 0.032283 | 0.047542 | 0.001351 | 0.099255 | 0.034020 | 0.045879 | 0.001371 | 0.093122 |
Mitotic Spindle | 0.001909 | 0.019308 | 0.001545 | 0.060697 | 0.005903 | 0.004027 | 0.000590 | 0.055250 | 0.004022 | 0.004953 | 0.000754 | 0.057080 |
None | 0.002742 | 0.001870 | 0.000436 | 0.001865 | 0.003400 | 0.002766 | 0.002214 | 0.003097 | 0.003090 | 0.002712 | 0.001908 | 0.002683 |
Nuclear Periphery | 0.002822 | 0.000603 | 0.000223 | 0.007829 | 0.004955 | 0.005211 | 0.000476 | 0.005984 | 0.003950 | 0.004931 | 0.000432 | 0.006604 |
Nuclear Periphery Foci | 0.001789 | 0.001606 | 0.000059 | 0.005729 | 0.002187 | 0.001696 | 0.000251 | 0.003817 | 0.001999 | 0.001690 | 0.000217 | 0.004459 |
Nucleolus | 0.021334 | 0.032218 | 0.003185 | 0.016291 | 0.020817 | 0.027261 | 0.004483 | 0.008555 | 0.021061 | 0.027562 | 0.004260 | 0.011154 |
Nucleus | 0.801796* | 0.768846* | 0.970642* | 0.506812* | 0.787645* | 0.747622* | 0.963916* | 0.429089* | 0.794311* | 0.748908* | 0.965075* | 0.455198* |
Peroxisomes | 0.008004 | 0.013037 | 0.000410 | 0.007603 | 0.006821 | 0.005920 | 0.000243 | 0.011340 | 0.007378 | 0.006351 | 0.000272 | 0.010085 |
Vacuole | 0.009482 | 0.010425 | 0.001579 | 0.035804 | 0.011139 | 0.020586 | 0.001448 | 0.046652 | 0.010358 | 0.019971 | 0.001470 | 0.043008 |
Vacuole Periphery | 0.002631 | 0.008614 | 0.000099 | 0.006767 | 0.002958 | 0.006819 | 0.000099 | 0.006968 | 0.002804 | 0.006927 | 0.000099 | 0.006901 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.55 | 11.10 | -8.20 | -5.84 | -9.43 | -1.10 | 9.46 | -13.34 | -13.12 | -15.14 | -1.48 | 14.40 | -15.58 | -15.11 | -17.76 |
Bud Neck | -2.13 | 7.39 | -17.38 | -7.81 | -20.18 | -3.88 | 11.21 | -21.73 | -19.07 | -27.71* | -6.03 | 13.12 | -28.12 | -22.39 | -34.17* |
Bud Site | -1.82 | 9.16 | -12.77 | -8.31 | -15.05 | 0.17 | 11.96 | -21.74 | -21.84 | -25.26* | -1.46 | 14.51 | -25.24 | -24.29 | -29.14* |
Cell Periphery | 4.42 | 9.02 | 0.60 | -5.14 | -13.95 | -0.43 | 9.08 | -2.67 | -0.42 | -13.56 | -0.57 | 11.08 | -2.44 | -0.04 | -15.78 |
Cytoplasm | -0.99 | 2.04 | -4.75 | 0.48 | -5.65 | 1.40 | 1.39 | -6.46 | -7.11 | -7.40 | -0.31 | 0.80 | -8.21 | -5.99 | -8.57 |
Cytoplasmic Foci | -0.37 | 3.79 | -2.53 | -1.54 | -6.74 | -2.53 | 5.96 | -4.57 | -0.82 | -6.58 | -2.51 | 6.36 | -5.08 | -0.44 | -8.20 |
Eisosomes | -2.85 | 10.75 | -5.58 | 1.12 | -11.27 | -6.61 | 12.60 | -12.21 | -7.16 | -20.14 | -6.48 | 17.77 | -12.94 | -6.43 | -23.19 |
Endoplasmic Reticulum | 4.66 | 24.01 | 3.88 | -2.00 | -15.31 | 2.44 | 25.06 | 1.38 | -0.84 | -21.08* | 3.79 | 35.19 | 3.29 | -0.39 | -25.93 |
Endosome | 0.62 | 11.22 | -4.86 | -4.47 | -7.19 | -3.03 | 9.07 | -5.92 | -2.03 | -9.80 | -3.66 | 12.72 | -7.81 | -1.95 | -11.97 |
Golgi | 0.37 | 2.01 | -1.73 | -2.40 | -4.15 | -1.83 | 4.68 | -3.97 | -2.97 | -4.76 | -1.73 | 3.80 | -4.24 | -2.87 | -5.51 |
Lipid Particles | 0.56 | 6.47 | -2.89 | -2.61 | -9.51 | -0.71 | 5.94 | -2.49 | -0.91 | -12.03 | -0.78 | 8.74 | -3.76 | -1.16 | -15.34 |
Mitochondria | 3.03 | 12.10 | -5.64 | -7.22 | -12.57 | -3.85 | 12.52 | -10.87 | -7.39 | -19.38* | -3.41 | 17.41 | -12.11 | -7.94 | -23.08* |
Mitotic Spindle | -1.81 | -0.37 | -8.43 | -3.12 | -7.54 | 1.35 | 4.09 | -10.03 | -10.66 | -11.52 | -0.78 | 3.19 | -13.31 | -12.84 | -14.13 |
None | 0.78 | 3.14 | 1.28 | 0.24 | -5.22 | 0.84 | 1.57 | 0.73 | -0.02 | -0.81 | 0.61 | 1.98 | 1.07 | 0.50 | -1.02 |
Nuclear Periphery | 8.40 | 10.36 | -4.86 | -7.38 | -7.80 | -0.35 | 9.10 | -1.34 | -1.00 | -7.70 | -1.68 | 12.22 | -4.11 | -2.13 | -10.61 |
Nuclear Periphery Foci | 0.12 | 3.95 | -3.99 | -3.24 | -6.21 | 1.11 | 4.23 | -2.74 | -4.51 | -7.93 | 0.90 | 5.40 | -4.87 | -5.98 | -9.78 |
Nucleolus | -1.38 | 14.67 | 2.81 | 2.02 | -9.10 | -3.42 | 11.86 | 8.67 | 12.11 | -3.94 | -3.98 | 17.25 | 9.29 | 10.79 | -8.09 |
Nucleus | 1.35 | -22.39 | 20.60 | 9.09 | 33.56 | 4.41 | -27.97 | 31.38 | 26.88 | 54.47* | 5.85 | -36.87 | 38.06 | 28.80 | 63.78 |
Peroxisomes | -1.51 | 7.92 | 0.55 | 1.73 | -8.04 | 0.88 | 7.26 | -2.01 | -2.94 | -8.18 | 1.17 | 10.79 | -1.50 | -2.53 | -10.49 |
Vacuole | -0.34 | 10.10 | -9.90 | -7.38 | -12.79 | -5.67 | 11.28 | -16.22 | -10.93 | -21.64 | -6.55 | 15.66 | -19.27 | -11.57 | -25.11 |
Vacuole Periphery | -0.84 | 6.45 | -2.80 | 0.40 | -5.55 | -4.56 | 7.31 | -3.24 | 0.79 | -7.04 | -4.77 | 9.74 | -4.31 | 1.04 | -8.90 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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