Standard name
Human Ortholog
Description Ribosome-associated protein; proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0.09 0 0 0.05 0 0 0 0 0 0.06 0.06 0 0.07 0.18 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.11 0.2 0.2 0.24 0.33 0 0 0 0 0 0
Cytoplasm 0 0.06 0.11 0.16 0.12 0.09 0.1 0.12 0.13 0.13 0.15 0.13 0 0 0.11 0.31 0.24 0.28 0 0 0 0 0 0
Endoplasmic Reticulum 0.89 0.9 0.74 0.67 0.8 0.78 0.56 0.56 0.47 0.41 0.39 0.43 0.95 0.88 0.75 0 0 0 0.95 0.94 0.9 0.89 0.83 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.06 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0.19 0.21 0.25 0 0 0 0 0 0
Mitochondria 0 0 0.21 0.16 0.07 0.18 0.29 0.2 0.3 0.25 0.37 0.39 0 0 0 0.07 0.13 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0.07 0.07 0 0 0.07 0.06 0.08 0.09 0.06 0.06 0 0 0 0 0.08 0.08 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0.06 0.1 0.08 0.09 0.25 0.22 0.19 0.29 0.19 0.17 0 0.06 0.12 0.18 0.05 0.08 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 10 18 11 12 26 21 14 17 10 15 6 14 8 7 7 3 0 1 3 0 1 2
Bud 1 0 0 1 2 5 13 8 9 8 13 13 0 0 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 5 2 4 14 12 17 19 21 24 19 25 4 25 55 20 9 12 0 0 1 0 1 2
Cytoplasm 9 11 13 31 42 25 52 65 58 79 53 86 4 8 30 31 9 10 4 3 2 1 4 5
Endoplasmic Reticulum 186 157 86 132 291 223 305 311 213 244 139 275 103 209 211 3 0 0 204 179 110 81 140 192
Endosome 0 0 0 1 0 0 2 2 4 4 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 12 3 4 18 5 0 0 1 0 0 0 2 0 6 5 19 8 9 1 0 0 0 2 1
Mitochondria 2 0 24 32 27 51 159 113 136 150 133 250 1 1 1 7 5 2 0 0 1 1 2 2
Nucleus 1 0 0 0 0 1 2 4 3 5 1 2 0 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 14 1 8 13 10 11 38 35 34 51 20 40 2 4 5 3 3 3 0 0 0 0 2 1
Nucleolus 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 23 8 7 19 29 27 136 121 86 168 70 108 2 14 33 18 2 3 1 3 1 2 7 13
Unique Cell Count 209 175 117 198 363 287 547 556 450 589 359 640 108 237 282 99 38 36 215 191 123 91 169 224
Labelled Cell Count 255 189 155 269 431 367 751 701 579 751 458 816 122 281 350 109 43 42 215 191 123 91 169 224


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.0 18.9 12.8 13.0 11.5 9.8 8.8 9.2 8.8 8.4 8.4 8.4 14.9 14.8 14.7 18.1 19.9 20.0 16.5 16.7 17.7
Std Deviation (1e-4) 3.2 3.1 2.2 2.7 2.0 1.7 1.7 1.7 1.6 1.6 1.6 1.5 2.4 2.7 2.4 4.5 4.0 4.5 2.5 2.8 3.2
Intensity Change (Log2) – – – 0.03 -0.15 -0.38 -0.53 -0.47 -0.54 -0.61 -0.6 -0.6 0.22 0.21 0.2 0.5 0.64 0.65 0.37 0.39 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -2.5 -1.8 -1.6 -2.2 0 0 0 0 -0.9 -0.9 -2.5 -0.4 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1.6 0 0 2.9 4.6 4.5 0 0
Cytoplasm 1.1 0.1 -0.7 -0.5 0.2 0.5 0.7 1.0 0.7 -2.1 -2.9 -0.1 3.7 1.9 2.4
Endoplasmic Reticulum -1.3 1.5 0.9 -3.5 -3.5 -5.1 -6.4 -6.5 -6.1 4.5 3.5 0.3 -10.5 -7.9 -7.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.9 0 0 0 0 0 0 0 0 0 0 0 3.7 0 0
Mitochondria -1.0 -4.0 -0.6 1.9 0 2.1 1.1 3.3 3.8 -4.7 -6.9 -7.6 -2.8 -1.0 -2.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.1 0 -1.3 0 -0.2 0.3 0.7 -0.5 -0.2 0 0 0 -1.3 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 0.7 1.1 4.5 4.0 3.4 5.2 3.4 3.0 0 0 1.7 2.8 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0009 0.0003 0.0001 0 0.0001 0.0004 0.0638 0.0002 0.0008 0.0002 0.0003 0.0001 – – – – – –
Bud 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 – – – – – –
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0003 0.0004 0.0002 0.0001 0.0001 0.0008 0 0.0001 – – – – – –
Bud Periphery 0 0 0 0 0 0.0001 0.0002 0 0.0003 0 0 0 – – – – – –
Bud Site 0 0.0001 0 0 0 0 0.0006 0.0001 0.0003 0 0 0 – – – – – –
Cell Periphery 0.0007 0.001 0.0009 0.0004 0.0007 0.0003 0.0003 0.0024 0.0009 0.0004 0.0003 0.0001 – – – – – –
Cytoplasm 0.01 0.0293 0.0189 0.0037 0.004 0.0104 0.0095 0.0192 0.0033 0.0279 0.0015 0.0017 – – – – – –
Cytoplasmic Foci 0.0114 0.0003 0.0003 0 0.0001 0.0011 0.0149 0.0006 0.0002 0.0007 0.0104 0.0027 – – – – – –
Eisosomes 0 0 0 0 0 0 0.0003 0 0.0001 0 0 0 – – – – – –
Endoplasmic Reticulum 0.9246 0.9518 0.9661 0.9872 0.9832 0.9738 0.8256 0.9518 0.9741 0.9634 0.9388 0.9792 – – – – – –
Endosome 0.0087 0.0013 0.0033 0.0008 0.0013 0.0032 0.038 0.0015 0.0032 0.0014 0.0031 0.0039 – – – – – –
Golgi 0.001 0.0002 0.0013 0 0.0001 0.0004 0.0099 0.0006 0.001 0.0002 0.0011 0.0053 – – – – – –
Lipid Particles 0.0048 0.0014 0.0028 0.0001 0.0006 0.0011 0.0103 0.0142 0.001 0.0003 0.0047 0.0002 – – – – – –
Mitochondria 0.0004 0 0.0001 0.0001 0.0001 0.0002 0.0013 0.0001 0.003 0 0.0002 0.0005 – – – – – –
None 0.0012 0 0 0 0 0.0002 0.0036 0.0001 0.0001 0.0001 0.0001 0 – – – – – –
Nuclear Periphery 0.0249 0.011 0.0045 0.0058 0.0067 0.0055 0.0097 0.0051 0.0082 0.0033 0.0023 0.004 – – – – – –
Nucleolus 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0 – – – – – –
Nucleus 0.0026 0.001 0.0002 0.0003 0.0006 0.0008 0.0004 0.0002 0.0003 0.0002 0.0001 0.0002 – – – – – –
Peroxisomes 0.0029 0 0 0 0 0.0001 0.0054 0 0 0 0.0359 0.0009 – – – – – –
Punctate Nuclear 0.0024 0 0 0 0 0.0006 0.0008 0 0 0 0.0002 0 – – – – – –
Vacuole 0.0025 0.002 0.0011 0.0013 0.002 0.0014 0.0038 0.0036 0.0022 0.0009 0.0009 0.0008 – – – – – –
Vacuole Periphery 0.0008 0.0002 0.0002 0.0002 0.0003 0.0002 0.0012 0.0002 0.0008 0.0001 0.0002 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.1246 84.253 64.0442 59.3754 58.1186 68.9246 58.6967 58.8986 73.9334 54.385
Translational Efficiency 1.3244 1.0133 1.2607 1.6335 1.2877 0.934 1.2176 1.2537 0.9308 1.2082

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1138 1140 2643 1265 1456 1936 1813 1275 2594 3076 4456 2540

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1058.02 1403.87 1653.27 1589.43 1148.27 1387.42 1693.69 1571.39 1108.68 1393.52 1669.72 1580.37
Standard Deviation 182.04 281.79 266.89 270.36 217.55 241.88 247.21 272.66 207.62 257.52 259.83 271.66
Intensity Change Log 2 — 0.408042 0.643955 0.587143 — 0.272943 0.560708 0.452579 — 0.339310 0.601229 0.518676

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002255 0.000406 0.000249 0.002133 0.000832 0.000336 0.000183 0.001035 0.001456 0.000362 0.000222 0.001581
Bud Neck 0.000272 0.000273 0.000405 0.005986 0.000239 0.000083 0.000217 0.001709 0.000254 0.000154 0.000329 0.003839
Bud Site 0.003030 0.004746 0.001572 0.021436 0.006819 0.002027 0.001042 0.010532 0.005157 0.003035 0.001356 0.015963
Cell Periphery 0.002703 0.001774 0.001852 0.009640 0.002083 0.001079 0.001704 0.012384 0.002355 0.001336 0.001792 0.011017
Cytoplasm 0.004140 0.008843 0.010348 0.033279 0.011082 0.003751 0.002744 0.017977 0.008036 0.005638 0.007254 0.025598
Cytoplasmic Foci 0.002203 0.005377 0.000585 0.005654 0.009846 0.003100 0.000423 0.003246 0.006493 0.003944 0.000519 0.004445
Eisosomes 0.000076 0.000016 0.000016 0.000105 0.000026 0.000020 0.000033 0.000080 0.000048 0.000018 0.000023 0.000092
Endoplasmic Reticulum 0.965780* 0.937561* 0.966909* 0.783034* 0.931360* 0.973963* 0.983934* 0.887514* 0.946460* 0.960472* 0.973836* 0.835480*
Endosome 0.004697 0.009377 0.003531 0.018070 0.008318 0.004301 0.002226 0.011003 0.006729 0.006182 0.003000 0.014523
Golgi 0.001537 0.003592 0.000795 0.009193 0.005064 0.002563 0.000603 0.004937 0.003517 0.002944 0.000717 0.007057
Lipid Particles 0.001662 0.001967 0.002285 0.027526 0.002694 0.001400 0.001385 0.020326 0.002242 0.001610 0.001919 0.023912
Mitochondria 0.001450 0.001145 0.000311 0.001044 0.001372 0.000760 0.000135 0.000593 0.001406 0.000902 0.000239 0.000818
Mitotic Spindle 0.000081 0.000096 0.000518 0.006400 0.000199 0.000077 0.000056 0.000686 0.000147 0.000084 0.000330 0.003531
None 0.000472 0.001033 0.001511 0.001515 0.002377 0.001110 0.000603 0.001791 0.001541 0.001081 0.001142 0.001654
Nuclear Periphery 0.000776 0.002421 0.001119 0.003268 0.001434 0.000223 0.000549 0.000759 0.001145 0.001038 0.000887 0.002008
Nuclear Periphery Foci 0.003182 0.009208 0.004548 0.036615 0.008061 0.000996 0.002114 0.010021 0.005921 0.004040 0.003558 0.023265
Nucleolus 0.000106 0.000118 0.000005 0.000076 0.000096 0.000005 0.000002 0.000021 0.000100 0.000047 0.000004 0.000049
Nucleus 0.001610 0.000479 0.000294 0.002281 0.001087 0.000086 0.000097 0.000619 0.001316 0.000232 0.000214 0.001446
Peroxisomes 0.000767 0.000105 0.000043 0.000211 0.000470 0.000127 0.000020 0.000294 0.000600 0.000119 0.000034 0.000252
Vacuole 0.002295 0.008021 0.002811 0.029845 0.003994 0.002785 0.001795 0.012170 0.003249 0.004725 0.002397 0.020972
Vacuole Periphery 0.000906 0.003445 0.000293 0.002689 0.002546 0.001206 0.000134 0.002306 0.001827 0.002036 0.000228 0.002497

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.06 2.20 -0.00 -3.63 -3.91 1.75 2.41 -0.79 -2.68 -3.36 2.57 2.87 -0.46 -4.50 -5.00
Bud Neck -0.05 -1.13 -7.31 -7.35 -7.12 4.26 -0.03 -5.79 -6.37 -5.66 1.59 -1.28 -8.65 -8.91 -8.38
Bud Site -1.36 1.74 -7.96 -6.85 -8.59 3.71 4.84 -2.16 -5.04 -5.78 2.37 4.92 -7.30 -8.82 -10.26
Cell Periphery 1.35 1.19 -5.60 -7.11 -6.99 2.62 0.88 -8.39 -9.62 -8.99 2.79 1.37 -10.03 -12.07 -11.27
Cytoplasm -3.16 -4.90 -10.39 -8.35 -7.96 4.55 5.10 -3.06 -7.23 -7.68 2.11 0.32 -9.62 -11.46 -10.28
Cytoplasmic Foci -2.57 2.43 -3.43 -0.28 -6.22 4.77 7.40 4.84 -0.22 -5.07 2.68 7.73 2.20 -0.76 -7.99
Eisosomes 2.64 2.58 -1.09 -5.63 -5.51 1.27 -0.54 -4.70 -5.31 -2.93 2.85 2.15 -3.24 -7.58 -6.37
Endoplasmic Reticulum 4.69 0.11 20.11 16.33 21.03 -8.02 -10.32 6.06 13.25 15.11 -3.48 -7.54 18.68 21.78 25.01
Endosome -5.15 2.46 -12.61 -6.89 -14.07 5.70 9.99 -3.03 -8.03 -11.48 0.99 9.28 -11.05 -11.39 -17.79
Golgi -2.10 1.74 -4.42 -2.88 -4.93 2.36 5.01 0.07 -2.70 -6.31 0.79 5.22 -3.39 -3.95 -6.96
Lipid Particles -0.48 -1.16 -10.41 -10.24 -10.10 2.62 2.34 -8.42 -9.08 -9.02 1.59 0.55 -13.34 -13.75 -13.44
Mitochondria 0.56 2.61 0.81 0.22 -4.42 1.80 4.61 2.77 0.69 -4.76 1.68 4.79 2.21 0.34 -6.01
Mitotic Spindle -0.36 -1.52 -4.90 -4.89 -4.54 1.29 1.34 -1.75 -2.23 -2.25 1.12 -1.10 -5.11 -5.19 -4.76
None -1.40 -2.64 -1.81 -0.71 0.04 1.78 2.40 0.67 -1.05 -1.74 1.06 0.89 -0.17 -1.23 -1.06
Nuclear Periphery -2.23 -1.81 -5.02 -1.10 -4.35 3.40 2.36 1.77 -5.21 -1.47 0.31 1.07 -2.83 -2.80 -4.34
Nuclear Periphery Foci -4.38 -1.66 -11.29 -9.02 -10.92 6.05 4.60 -1.30 -6.83 -5.59 2.19 2.64 -10.07 -11.68 -11.94
Nucleolus -0.11 1.76 0.12 0.20 -2.41 2.16 2.23 1.76 -3.59 -4.29 1.08 2.80 1.03 -0.31 -2.89
Nucleus 1.26 1.47 -1.34 -5.99 -6.87 1.82 1.74 0.77 -6.18 -5.45 2.19 2.17 -0.83 -7.74 -7.86
Peroxisomes 2.40 2.62 2.00 -2.73 -4.74 3.16 4.24 1.35 -2.09 -3.57 3.55 4.20 2.46 -2.99 -5.16
Vacuole -3.92 -0.89 -11.40 -8.49 -11.50 1.32 2.56 -6.01 -6.61 -7.51 -1.99 1.14 -12.74 -11.19 -13.54
Vacuole Periphery -2.92 2.85 -4.57 0.81 -6.76 2.56 5.56 0.29 -1.48 -3.17 -0.49 6.05 -1.49 -0.88 -5.86
Standard name
Human Ortholog
Description Ribosome-associated protein; proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Snl1

Snl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available