Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0.06 0.07 0.09 0.13 0.09 – 0.05 0 0.08 0.1 0.11 0.13 0.17 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.22 0.26 0.16 0.31 0.39 0.33 0.42 – 0.42 0.5 0 0 0.07 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0.89 0.93 0.89 0.9 0.88 0.78 0.77 0.65 0.65 – 0.73 0.7 0.86 0.85 0.8 0.77 0.72 0.72 0.79 0.89 0.85 0.81 0.69 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0.16 0.16 0.08 0.14 0.16 0.2 0.18 – 0.29 0.33 0.13 0.14 0.15 0.07 0.06 0.08 0.12 0.05 0.07 0.1 0.23 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.05 0 0 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 2 0 0
Bud 0 0 2 0 0 0 1 2 1 – 1 2 0 1 1 0 0 1 4 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 2 1 0 4 4 3 8 – 7 24 1 1 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 16 4 3 3 10 9 16 16 17 – 10 7 26 41 49 36 30 39 0 0 0 0 2 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 7 4 2 0 0 2 2 1 0
Endosome 1 1 0 0 1 0 0 0 0 – 0 0 0 0 0 4 4 3 2 2 0 3 3 9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 1 0 2 0 1 1 2 1 0 0 0 3
Mitochondria 2 35 52 21 26 38 73 39 75 – 85 156 7 6 31 14 2 11 4 2 1 1 1 5
Nucleus 287 402 210 73 146 95 142 77 117 – 148 216 284 364 374 209 125 159 280 403 204 264 259 215
Nuclear Periphery 1 6 1 1 2 1 6 0 4 – 2 4 1 3 4 0 0 0 0 0 1 1 0 0
Nucleolus 38 40 37 13 14 17 30 24 32 – 60 101 43 59 69 20 10 17 41 22 16 32 84 79
Peroxisomes 1 0 1 0 0 0 0 0 0 – 0 0 1 2 3 0 0 1 0 0 0 1 0 0
SpindlePole 2 1 1 0 0 0 0 0 0 – 1 1 4 6 3 4 2 0 4 2 1 0 3 5
Vac/Vac Membrane 3 2 2 1 1 3 0 1 2 – 1 2 1 1 3 14 8 11 9 14 10 13 15 25
Unique Cell Count 321 434 235 81 166 122 185 119 180 204 310 330 428 465 271 173 220 353 452 240 329 377 355
Labelled Cell Count 351 491 312 113 200 167 272 162 256 315 513 370 484 543 308 186 245 353 452 240 329 377 355


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 6.3 5.2 5.1 5.9 5.0 4.8 4.7 4.8 – 4.4 4.1 6.8 6.9 6.0 8.9 9.8 10.7 7.2 7.5 7.7
Std Deviation (1e-4) 2.4 1.2 1.2 1.6 2.1 1.7 1.6 1.7 2.4 – 1.5 1.4 1.7 1.7 1.6 2.3 2.7 2.8 1.4 2.0 1.9
Intensity Change (Log2) – – – -0.03 0.18 -0.05 -0.11 -0.12 -0.11 – -0.22 -0.35 0.39 0.42 0.22 0.79 0.93 1.05 0.47 0.53 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 2.6 0 3.6 4.8 3.9 – 2.2 0 3.5 4.1 4.4 5.1 5.9 6.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.7 -1.6 1.9 0 0 0 – 0 0 -7.7 -9.0 -6.0 -5.7 -6.2 -5.3
Nucleus 0.2 -0.4 -2.9 -3.5 -5.6 -6.0 – -4.5 -5.5 -1.2 -1.6 -3.0 -3.6 -4.5 -4.7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.1 -2.2 -0.5 0 0 0 – 0 0 -0.9 -0.7 -0.3 -3.0 -3.1 -2.6
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 2.8 0 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.4406 6.245 6.0815 5.6602 5.3227 6.1704 3.1947 2.9196 2.8131 2.4728 2.5867 2.5088 7.2993 6.5458 6.378 6.4367 6.3761 6.7788
Actin 0 0 0 0 0 0 0.0179 0 0 0 0 0 0.0005 0 0.0007 0.0001 0.0002 0.0002
Bud 0 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0.0005 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0 0.0001 0 0.0001 0.001 0.0006 0.0011 0 0 0 0.0002 0.0003 0.0015 0 0.0001 0.0003 0.0002 0.0007
Bud Periphery 0 0 0 0 0.0002 0 0.0003 0 0 0 0 0.0001 0.0013 0 0.0002 0.0002 0.0001 0.0002
Bud Site 0 0.0001 0 0 0.0003 0.0001 0.0007 0 0 0 0 0 0.0026 0 0.0002 0 0.0001 0.0003
Cell Periphery 0 0 0 0 0.0002 0 0.0002 0 0 0 0 0 0.0005 0 0.0001 0 0 0.0001
Cytoplasm 0 0 0 0 0 0 0.0007 0 0 0 0 0 0.0017 0 0.0003 0.0001 0.0014 0
Cytoplasmic Foci 0 0 0 0 0 0 0.0023 0 0 0 0 0 0.0132 0 0.0121 0 0.0116 0.0018
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0011 0 0 0 0 0 0.0019 0 0.0002 0 0.0015 0
Endosome 0 0 0 0 0 0 0.0067 0 0 0 0 0 0.0122 0 0.0097 0.0001 0.0411 0.0012
Golgi 0 0 0 0 0 0 0.0028 0 0 0 0 0 0.0023 0 0.0033 0 0.0033 0.0009
Lipid Particles 0 0 0 0 0 0 0.0043 0 0 0 0 0 0.0399 0 0.01 0 0.0065 0.0086
Mitochondria 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0111 0 0 0 0.0002 0.0001 0.0026 0.0001 0.0333 0.0003 0.0011 0.0008
None 0 0 0 0 0 0 0.0008 0 0 0 0 0 0.0029 0 0.0001 0.0001 0.0005 0.0001
Nuclear Periphery 0.0001 0.0004 0.0003 0.0001 0.0002 0.0001 0.0106 0.0002 0.0001 0.0001 0.0002 0.0001 0.0161 0.001 0.0008 0.0052 0.0013 0.0004
Nucleolus 0.0331 0.0335 0.0239 0.0287 0.2131 0.0836 0.0228 0.0194 0.0132 0.0147 0.0838 0.0712 0.0674 0.0207 0.0203 0.0465 0.1008 0.1065
Nucleus 0.9662 0.9642 0.9754 0.9705 0.7815 0.9153 0.9077 0.9802 0.9866 0.985 0.9151 0.9277 0.7764 0.9776 0.9044 0.9435 0.8264 0.8722
Peroxisomes 0 0 0 0 0 0 0.0011 0 0 0 0 0 0.0023 0 0.0017 0 0.0008 0.004
Punctate Nuclear 0.0001 0.0015 0.0001 0.0003 0.0022 0.0001 0.0015 0.0002 0 0.0001 0.0002 0.0002 0.0467 0.0001 0.0018 0.0031 0.0009 0.0016
Vacuole 0.0001 0.0001 0 0.0001 0.0007 0.0001 0.0026 0 0 0 0.0001 0.0001 0.0057 0.0001 0.0005 0.0002 0.0014 0.0002
Vacuole Periphery 0 0 0 0 0.0001 0 0.0036 0 0 0 0 0 0.0019 0.0001 0.0004 0.0002 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4515 15.5393 16.1066 19.5957 14.8463 15.1672 18.0187 18.8523 21.3948 18.9234
Translational Efficiency 0.9507 0.9665 0.8576 0.8125 0.7763 0.6375 0.8006 0.5862 0.5816 0.6495

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2253 1000 437 1143 1134 1270 1025 723 3387 2270 1462 1866

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 740.72 919.01 1220.05 1043.84 809.75 911.63 996.67 1080.89 763.83 914.88 1063.44 1058.20
Standard Deviation 102.28 140.13 155.37 171.78 124.07 135.48 143.48 176.43 114.77 137.59 179.18 174.53
Intensity Change Log 2 — 0.311152 0.719940 0.494900 — 0.170972 0.299639 0.416671 — 0.239642 0.515721 0.454574

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000213 0.000613 0.001005 0.001203 0.000498 0.000557 0.000813 0.000716 0.000308 0.000582 0.000870 0.001014
Bud Neck 0.003995 0.008813 0.018892 0.030043 0.006820 0.010307 0.012547 0.020595 0.004941 0.009649 0.014443 0.026382
Bud Site 0.000551 0.001111 0.001208 0.005219 0.001407 0.001975 0.001038 0.001542 0.000838 0.001594 0.001089 0.003794
Cell Periphery 0.000142 0.000040 0.000039 0.000129 0.000066 0.000065 0.000053 0.000074 0.000117 0.000054 0.000049 0.000108
Cytoplasm 0.001561 0.000388 0.000196 0.001660 0.001905 0.000558 0.001084 0.000473 0.001676 0.000483 0.000818 0.001200
Cytoplasmic Foci 0.000204 0.000334 0.000023 0.001395 0.001157 0.001236 0.000034 0.000019 0.000523 0.000839 0.000031 0.000862
Eisosomes 0.000025 0.000038 0.000053 0.000038 0.000034 0.000046 0.000051 0.000033 0.000028 0.000042 0.000051 0.000036
Endoplasmic Reticulum 0.002825 0.002716 0.002035 0.003026 0.002666 0.003810 0.003174 0.002108 0.002772 0.003328 0.002833 0.002670
Endosome 0.000185 0.000515 0.000069 0.004031 0.000173 0.001319 0.000094 0.000120 0.000181 0.000965 0.000086 0.002516
Golgi 0.000057 0.000422 0.000113 0.001830 0.000437 0.000623 0.000081 0.000260 0.000185 0.000535 0.000090 0.001222
Lipid Particles 0.000084 0.000149 0.000046 0.000236 0.000249 0.000437 0.000051 0.000021 0.000140 0.000310 0.000049 0.000153
Mitochondria 0.000611 0.002123 0.001337 0.003403 0.000783 0.003427 0.001011 0.002183 0.000669 0.002852 0.001108 0.002930
Mitotic Spindle 0.000120 0.003784 0.001626 0.013357 0.001614 0.005034 0.000897 0.001304 0.000620 0.004483 0.001115 0.008687
None 0.005397 0.001461 0.001958 0.005334 0.002666 0.001543 0.002697 0.001685 0.004482 0.001507 0.002476 0.003920
Nuclear Periphery 0.000190 0.000235 0.000134 0.001933 0.000116 0.000708 0.000144 0.000358 0.000165 0.000500 0.000141 0.001323
Nuclear Periphery Foci 0.000285 0.000465 0.000033 0.001059 0.000411 0.000640 0.000067 0.000060 0.000327 0.000563 0.000057 0.000672
Nucleolus 0.037985 0.045060 0.041668 0.015065 0.051837 0.056854 0.036316 0.013661 0.042622 0.051658 0.037915 0.014521
Nucleus 0.944694* 0.929476* 0.927754* 0.899569* 0.925809* 0.905857* 0.938618* 0.953066* 0.938371* 0.916262* 0.935371* 0.920297*
Peroxisomes 0.000278 0.001367 0.001039 0.000465 0.000877 0.000875 0.000594 0.000243 0.000478 0.001091 0.000727 0.000379
Vacuole 0.000428 0.000612 0.000627 0.006828 0.000265 0.002295 0.000519 0.000808 0.000373 0.001554 0.000551 0.004496
Vacuole Periphery 0.000170 0.000277 0.000146 0.004177 0.000211 0.001835 0.000119 0.000672 0.000184 0.001149 0.000127 0.002819

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.65 -14.55 -8.02 -4.82 -0.89 -0.16 -3.57 -3.58 -4.23 -0.33 -4.74 -15.52 -9.09 -5.95 -2.16
Bud Neck -5.40 -14.06 -15.38 -11.66 -4.84 -3.77 -6.03 -9.54 -7.10 -5.23 -7.58 -13.61 -18.21 -13.51 -8.61
Bud Site -2.17 -6.71 -4.87 -3.94 -3.35 -0.80 0.77 -1.08 -0.35 -1.68 -2.10 -2.39 -4.76 -2.97 -4.04
Cell Periphery 2.93 2.88 0.91 -5.20 -4.90 0.12 1.26 -0.38 -0.44 -1.04 2.61 2.87 0.81 -3.19 -3.76
Cytoplasm 3.45 4.61 -0.39 -4.33 -5.80 1.91 1.09 1.91 -0.09 0.90 3.61 2.02 1.21 -3.68 -1.33
Cytoplasmic Foci -1.01 2.25 -1.90 -1.04 -2.74 0.18 1.95 1.95 2.58 0.43 -0.73 2.45 0.42 1.21 -2.71
Eisosomes -5.50 -10.84 -7.73 -1.13 5.78 -3.50 -6.44 -0.55 3.48 7.17 -7.37 -14.17 -6.58 1.76 8.69
Endoplasmic Reticulum 0.38 2.23 -2.70 -3.24 -5.56 -2.34 -2.36 1.30 2.98 3.78 -1.82 -0.91 -1.73 0.71 -0.94
Endosome -1.00 3.55 -2.96 -2.26 -3.10 -3.70 2.66 1.07 3.87 -1.01 -3.42 4.22 -2.91 -1.06 -3.12
Golgi -2.24 -6.86 -2.86 -1.40 -2.61 -0.18 2.35 1.31 1.37 -1.04 -1.99 1.77 -2.25 -0.69 -2.86
Lipid Particles -1.30 0.93 -1.15 0.13 -1.58 -0.52 2.01 2.16 2.03 2.28 -1.35 2.26 0.51 1.62 -1.47
Mitochondria -2.25 -7.40 -8.47 -1.65 -5.77 -3.66 -2.25 -3.19 1.30 -2.66 -4.35 -5.92 -8.41 -0.21 -6.64
Mitotic Spindle -2.55 -1.99 -5.12 -2.93 -4.25 -1.81 0.24 -1.52 -0.03 -1.67 -3.48 -1.15 -5.27 -2.49 -4.45
None 5.19 3.93 1.07 -3.09 -2.40 1.85 -0.01 1.34 -0.72 1.01 5.35 3.06 1.59 -3.06 -1.33
Nuclear Periphery -0.45 0.59 -4.26 -3.98 -4.32 -2.38 -1.92 -4.46 0.65 -3.88 -2.27 -0.10 -4.90 -2.85 -4.90
Nuclear Periphery Foci -0.71 1.66 -1.50 -0.76 -2.64 -0.46 2.66 2.27 1.49 -1.26 -0.89 2.56 -0.82 0.19 -2.69
Nucleolus -2.59 -3.38 10.98 11.42 8.41 -1.39 4.33 11.15 12.80 6.52 -4.13 0.33 15.40 17.49 10.76
Nucleus 3.68 6.27 6.99 3.63 0.89 3.24 -2.29 -2.65 -5.54 -0.71 6.22 2.94 4.80 -0.54 2.27
Peroxisomes -2.15 -5.58 -1.37 1.92 5.23 0.51 1.50 2.29 1.93 6.15 -1.87 -1.60 1.31 2.59 6.52
Vacuole -0.86 -2.65 -4.39 -4.14 -4.02 -3.52 -2.11 -2.35 1.91 -1.39 -3.46 -2.20 -4.74 -2.61 -4.32
Vacuole Periphery -1.67 0.08 -2.68 -2.55 -2.69 -3.16 3.05 -0.82 2.01 -1.05 -3.33 1.66 -2.81 -0.92 -2.90
Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt7

Spt7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available