| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | G protein gamma subunit; forms dimer with Ste4p to activate mating signaling pathway, heterotrimer with Gpa1p, Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling; N-terminal phosphorylation of Ste18p functions synergistically with Fus3p docking site on Ste5p to restrict Ste5p plasma membrane recruitment and signaling; combinatorial phosphorylation at S3 and S7, by pheromone and pH stress, modulates Ste18p structure, Fus3p activation |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 0 | – | – | – | 0 |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | – | – | – | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | – | – | – | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Vac/Vac Membrane | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – | – | – | 0 |
| Unique Cell Count | 7 | 5 | 1 | |||||||||||||||||||||
| Labelled Cell Count | 7 | 5 | 1 | |||||||||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Std Deviation (1e-4) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 38.1056 | 8.8863 | 21.98 | 43.4629 | 38.6652 | 19.8702 | 47.9195 | 45.4815 | 42.7338 | 47.5642 |
| Translational Efficiency | 2.1211 | 9.6293 | 4.1866 | 1.8136 | 2.4199 | 4.7491 | 2.5254 | 3.3854 | 2.7793 | 2.6585 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 167 | 631 | 1635 | N/A | 872 | 717 | N/A | 39 | 1039 | 1348 | 1635 | 39 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 935.16 | 1002.84 | 1119.37 | N/A | 879.29 | 985.74 | N/A | 404.33 | 888.27 | 993.74 | 1119.37 | 404.33 |
| Standard Deviation | 121.73 | 149.87 | 167.74 | N/A | 160.55 | 138.60 | N/A | 20.69 | 156.32 | 144.24 | 167.74 | 20.69 |
| Intensity Change Log 2 | — | 0.100806 | 0.259402 | N/A | — | 0.164868 | N/A | -1.120806 | — | 0.132206 | — | — |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.011429 | 0.050426 | 0.044762 | N/A | 0.023369 | 0.023143 | N/A | 0.000391 | 0.021450 | 0.035914 | 0.044762 | 0.000391 |
| Bud Neck | 0.018195 | 0.014600 | 0.006890 | N/A | 0.009917 | 0.010280 | N/A | 0.000015 | 0.011248 | 0.012302 | 0.006890 | 0.000015 |
| Bud Site | 0.088556 | 0.129197* | 0.093719 | N/A | 0.048827 | 0.082356 | N/A | 0.000044 | 0.055213 | 0.104282* | 0.093719 | 0.000044 |
| Cell Periphery | 0.002075 | 0.004319 | 0.000829 | N/A | 0.003196 | 0.002699 | N/A | 0.001224 | 0.003016 | 0.003457 | 0.000829 | 0.001224 |
| Cytoplasm | 0.271241* | 0.209878* | 0.325964* | N/A | 0.100693* | 0.201376* | N/A | 0.000217 | 0.128105* | 0.205356* | 0.325964* | 0.000217 |
| Cytoplasmic Foci | 0.361946* | 0.341956* | 0.257027* | N/A | 0.382789* | 0.395542* | N/A | 0.000025 | 0.379439* | 0.370458* | 0.257027* | 0.000025 |
| Eisosomes | 0.002291 | 0.002767 | 0.000296 | N/A | 0.002312 | 0.001529 | N/A | 0.002232 | 0.002308 | 0.002109 | 0.000296 | 0.002232 |
| Endoplasmic Reticulum | 0.003886 | 0.008310 | 0.005408 | N/A | 0.005142 | 0.007976 | N/A | 0.000006 | 0.004940 | 0.008132 | 0.005408 | 0.000006 |
| Endosome | 0.057175 | 0.051009 | 0.038172 | N/A | 0.097547 | 0.054225 | N/A | 0.000001 | 0.091058 | 0.052720 | 0.038172 | 0.000001 |
| Golgi | 0.084019 | 0.099285 | 0.023435 | N/A | 0.250503* | 0.131819* | N/A | 0.000012 | 0.223744* | 0.116590* | 0.023435 | 0.000012 |
| Lipid Particles | 0.015530 | 0.013994 | 0.060301 | N/A | 0.025231 | 0.017966 | N/A | 0.000286 | 0.023671 | 0.016107 | 0.060301 | 0.000286 |
| Mitochondria | 0.001955 | 0.002294 | 0.000195 | N/A | 0.008016 | 0.008544 | N/A | 0.000034 | 0.007042 | 0.005618 | 0.000195 | 0.000034 |
| Mitotic Spindle | 0.004507 | 0.001856 | 0.016783 | N/A | 0.002428 | 0.009117 | N/A | 0.000524 | 0.002762 | 0.005718 | 0.016783 | 0.000524 |
| None | 0.000087 | 0.000729 | 0.000469 | N/A | 0.001800 | 0.000160 | N/A | 0.992757* | 0.001524 | 0.000426 | 0.000469 | 0.992757* |
| Nuclear Periphery | 0.000037 | 0.000024 | 0.000088 | N/A | 0.000150 | 0.000109 | N/A | 0.000001 | 0.000132 | 0.000070 | 0.000088 | 0.000001 |
| Nuclear Periphery Foci | 0.000894 | 0.000831 | 0.010907 | N/A | 0.001340 | 0.001149 | N/A | 0.000001 | 0.001268 | 0.001000 | 0.010907 | 0.000001 |
| Nucleolus | 0.000116 | 0.000114 | 0.000093 | N/A | 0.000758 | 0.000791 | N/A | 0.000002 | 0.000655 | 0.000474 | 0.000093 | 0.000002 |
| Nucleus | 0.005731 | 0.004537 | 0.006301 | N/A | 0.002807 | 0.003975 | N/A | 0.000011 | 0.003277 | 0.004238 | 0.006301 | 0.000011 |
| Peroxisomes | 0.017320 | 0.018782 | 0.020986 | N/A | 0.014789 | 0.015816 | N/A | 0.002216 | 0.015196 | 0.017205 | 0.020986 | 0.002216 |
| Vacuole | 0.050089 | 0.042305 | 0.086562 | N/A | 0.014426 | 0.027423 | N/A | 0.000000 | 0.020158 | 0.034390 | 0.086562 | 0.000000 |
| Vacuole Periphery | 0.002921 | 0.002789 | 0.000812 | N/A | 0.003961 | 0.004004 | N/A | 0.000000 | 0.003794 | 0.003435 | 0.000812 | 0.000000 |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -4.40 | -6.02 | 10.78 | 13.47 | 16.16 |
| Bud Neck | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -0.92 | 5.02 | 13.37 | 17.09 | 28.88* |
| Bud Site | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -9.71 | -7.74 | 17.48 | 26.51* | 30.52* |
| Cell Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -0.50 | 3.02 | 1.84 | 2.73 | -0.70 |
| Cytoplasm | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -9.67 | -23.59 | 23.41 | 35.06 | 48.65* |
| Cytoplasmic Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 1.06 | 17.29 | 62.49 | 60.55 | 53.00* |
| Eisosomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 0.60 | 7.23 | 0.08 | -0.16 | -2.42 |
| Endoplasmic Reticulum | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -2.64 | -0.03 | 6.68 | 8.58 | 11.12 |
| Endosome | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 8.53 | 13.63 | 23.36 | 23.14 | 23.26 |
| Golgi | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 14.34 | 31.15 | 34.68 | 30.26 | 18.40 |
| Lipid Particles | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 3.67 | -10.63 | 12.87 | 14.44 | 22.00 |
| Mitochondria | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 0.94 | 6.12 | 6.26 | 5.22 | 4.63 |
| Mitotic Spindle | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -1.98 | -7.64 | 2.95 | 4.00 | 9.82 |
| None | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 1.05 | 1.05 | -320.97* | -340.79* | -341.18* |
| Nuclear Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 1.29 | 0.68 | 3.20 | 2.59 | 19.20 |
| Nuclear Periphery Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 0.76 | -11.30 | 4.55 | 3.59 | 13.34 |
| Nucleolus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 0.54 | 2.62 | 3.09 | 1.57 | 18.14 |
| Nucleus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -0.85 | -4.69 | 3.62 | 5.06 | 19.61 |
| Peroxisomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -1.79 | -2.44 | 9.85 | 11.53 | 10.18 |
| Vacuole | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | -5.35 | -22.89 | 12.40 | 15.61 | 31.45 |
| Vacuole Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | 0.69 | 9.10 | 11.44 | 8.23 | 10.55 |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | G protein gamma subunit; forms dimer with Ste4p to activate mating signaling pathway, heterotrimer with Gpa1p, Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling; N-terminal phosphorylation of Ste18p functions synergistically with Fus3p docking site on Ste5p to restrict Ste5p plasma membrane recruitment and signaling; combinatorial phosphorylation at S3 and S7, by pheromone and pH stress, modulates Ste18p structure, Fus3p activation |
| Localization | |
| Cell Percentages | cytoplasm (17%), ER (5%), mixed (38%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Ste18
Ste18
Nearest neighbours ( genes)
correlation threshold
0.80
