Standard name
Human Ortholog
Description Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves both isoprenylated and non-prenylated oligopeptides; contains multiple transmembrane spans; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and can complement yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.36 0.56 0.19 0.34 0.58 0.63 0.62 0.64 0.69 0.69 0.67 0.72 0.25 0.36 0.25 0.51 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.48 0.41 0.8 0.68 0.4 0.36 0.34 0.31 0.29 0.28 0.29 0.24 0.81 0.66 0.12 0.05 0.84 0.77 0.92 0.86 0.88 0.89
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0 0.06 0 0.08 0 0.09 0.09 0 0.05 0.05 0.07 0 0 0.62 0.49 0.09 0.13 0.06 0.07 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 6 2 2 4 10 5 3 5 8 2 2 0 1 0 0 0 0 2 3
Bud 0 0 0 0 1 0 3 8 2 6 6 6 1 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 2 2 15 16 21 15 16 17 21 9 13 0 0 0 0 0 0 0 0
Cytoplasm 23 23 66 83 191 222 313 378 246 285 267 261 40 62 4 21 3 4 1 3 4 4
Endoplasmic Reticulum 31 17 272 168 132 126 173 182 103 117 114 86 130 114 2 2 95 56 354 121 277 249
Endosome 0 0 1 0 0 0 1 1 0 3 1 2 0 0 0 0 0 0 0 0 1 1
Golgi 0 0 5 1 0 1 1 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 1 0 0 0 2 0 2 1 0 0 0 1 0 0 0 0 0 1 1
Nucleus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 1 7 0 1 2 3 4 4 4 1 0 0 0 0 2 0 1 1 1 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 13 1 19 5 26 15 45 54 15 19 20 25 1 2 10 20 9 9 23 10 23 18
Unique Cell Count 64 41 341 247 328 350 505 587 354 412 397 363 161 172 16 41 114 73 384 141 317 282
Labelled Cell Count 67 42 368 273 355 383 558 660 390 455 436 411 183 193 19 45 114 73 384 141 317 282


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.5 20.8 24.8 23.7 22.6 19.7 18.2 17.5 16.0 14.4 14.3 15.3 19.5 19.6 19.0 20.5 23.7 25.6 27.8
Std Deviation (1e-4) 5.1 4.9 3.9 4.1 4.5 3.5 3.2 3.2 3.2 2.8 2.9 3.4 4.1 3.7 4.3 6.3 4.7 5.2 5.6
Intensity Change (Log2) -0.06 -0.13 -0.33 -0.45 -0.5 -0.63 -0.78 -0.8 -0.7 -0.35 -0.34 -0.38 -0.27 -0.06 0.05 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 1.4 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.5 2.9 3.2 3.5 3.3 3.5 4.2 0 0 0 0
Cytoplasm 3.9 10.3 11.7 12.2 13.2 13.3 13.6 13.0 14.0 1.4 4.1 0 4.6
Endoplasmic Reticulum -3.2 -10.4 -11.6 -13.0 -14.3 -13.4 -14.0 -13.8 -14.9 0.3 -3.3 -6.2 -10.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 1.2 -0.8 1.8 2.0 -0.8 -0.6 -0.3 0.7 -2.6 -2.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.4 23.4777 22.0707 20.2076 22.4162 22.5914 21.5695 27.8523 26.2269 24.9237 23.6328 25.612 22.7345 25.0574 22.645 19.0901 21.0587 25.2146
Actin 0.0041 0.0001 0.0079 0.0002 0.0012 0.0001 0.0202 0 0.0097 0.0001 0.0318 0.0009 0.0091 0.0001 0.0185 0.0698 0.0138 0.0022
Bud 0.0003 0.0001 0.003 0.001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0005 0.0003 0.0004 0.0001
Bud Neck 0.0012 0.0006 0.0005 0.0035 0.0008 0.0107 0.0003 0.0003 0.0049 0.0003 0.0008 0.0002 0.0008 0.0008 0.0007 0.001 0.001 0.0004
Bud Periphery 0.0006 0.0003 0.0097 0.0016 0.0003 0.0005 0.0004 0.0002 0.0003 0.0005 0.0006 0.0002 0.0003 0.0005 0.0007 0.0017 0.0008 0.0004
Bud Site 0.0017 0.0003 0.001 0.0057 0.0005 0.0001 0.0012 0.0001 0.002 0.0002 0.0002 0.0001 0.0008 0.0002 0.0032 0.0016 0.0006 0.0001
Cell Periphery 0.0091 0.0261 0.0163 0.0228 0.0069 0.0047 0.0083 0.027 0.0122 0.0106 0.0316 0.0061 0.0056 0.0092 0.0148 0.0398 0.0023 0.0072
Cytoplasm 0.0301 0.06 0.0366 0.0239 0.0322 0.0361 0.0115 0.022 0.0107 0.0135 0.0132 0.0058 0.0084 0.0284 0.0102 0.007 0.0448 0.0088
Cytoplasmic Foci 0.0067 0.001 0.0007 0.0005 0.001 0.0018 0.0084 0.0001 0.0036 0.0022 0.0026 0.0023 0.0099 0.0002 0.0092 0.0191 0.0021 0.0017
Eisosomes 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0003 0 0.0001 0 0.0008 0 0.0002 0.0019 0.001 0
Endoplasmic Reticulum 0.728 0.6632 0.7318 0.5576 0.645 0.7024 0.6903 0.7748 0.8081 0.7185 0.5181 0.7608 0.5352 0.4897 0.6028 0.1996 0.4436 0.6674
Endosome 0.0104 0.0048 0.0043 0.0021 0.0106 0.0071 0.0249 0.0013 0.0052 0.0107 0.0224 0.0116 0.025 0.0029 0.016 0.0267 0.0264 0.009
Golgi 0.0011 0.0019 0.0003 0.0001 0.002 0.0016 0.0044 0 0.0021 0.0004 0.006 0.003 0.031 0 0.0088 0.014 0.0049 0.0046
Lipid Particles 0.0063 0.0085 0.0075 0.0022 0.0062 0.0035 0.0194 0.0064 0.0093 0.005 0.0116 0.0042 0.0074 0.0021 0.0289 0.0557 0.0099 0.0117
Mitochondria 0.0013 0.002 0.0008 0.0001 0.0068 0.0002 0.0012 0 0.0097 0.0021 0.0011 0.0015 0.0024 0.0001 0.0263 0.003 0.0042 0.0031
None 0.0043 0.0003 0.0082 0.0001 0.0022 0 0.0008 0 0.0021 0.0013 0.0003 0.0001 0.0445 0.0001 0.0009 0.021 0.0123 0.0001
Nuclear Periphery 0.0525 0.0363 0.0389 0.0497 0.0627 0.0725 0.0833 0.0241 0.0325 0.0316 0.0876 0.0889 0.1616 0.0953 0.0492 0.0271 0.1886 0.1758
Nucleolus 0.016 0.0112 0.0075 0.0909 0.0016 0.0002 0.001 0.0001 0.0001 0.0005 0.0002 0.0001 0.003 0.0009 0.0009 0.001 0.001 0.0001
Nucleus 0.0206 0.009 0.0069 0.0493 0.0088 0.0109 0.0048 0.0014 0.001 0.0032 0.0098 0.004 0.0165 0.0297 0.0035 0.0028 0.0115 0.0043
Peroxisomes 0.0009 0.0001 0.0001 0 0.0001 0.0072 0.0033 0 0.0021 0.0002 0.0033 0.0006 0.0014 0 0.0161 0.0236 0.0017 0.002
Punctate Nuclear 0.0039 0.0004 0.0022 0.0007 0.0004 0.0001 0.0015 0 0.0004 0.0004 0.0005 0.0015 0.0117 0.0001 0.0005 0.0082 0.0037 0.0003
Vacuole 0.0943 0.1702 0.1103 0.1847 0.1997 0.1333 0.1025 0.1396 0.0796 0.1866 0.2407 0.0977 0.1118 0.3162 0.1685 0.4574 0.2003 0.0817
Vacuole Periphery 0.0065 0.0037 0.0053 0.0034 0.0105 0.0068 0.012 0.0025 0.0041 0.0119 0.0172 0.0102 0.0125 0.0234 0.0195 0.0177 0.0252 0.019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 102.8872 82.5601 84.3712 97.8574 86.656 77.7299 96.1078 100.0794 94.1621 106.2558
Translational Efficiency 1.0435 1.1021 1.0483 0.9181 1.0949 1.3199 1.006 0.8854 1.0975 0.9902

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
130 523 106 914 2372 1273 173 1396 2502 1796 279 2310

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2077.97 1782.49 2004.34 2856.47 1625.72 1766.28 2342.27 2618.68 1649.22 1771.00 2213.88 2712.77
Standard Deviation 389.46 345.38 243.84 618.43 256.52 320.19 385.14 506.86 283.44 327.81 376.13 565.78
Intensity Change Log 2 -0.221281 -0.052048 0.459059 0.119635 0.526829 0.687761 -0.061644 0.230927 0.563935

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000202 0.000179 0.000077 0.000594 0.000175 0.000086 0.000152 0.000506 0.000176 0.000113 0.000123 0.000541
Bud Neck 0.002035 0.000600 0.007221 0.003061 0.000070 0.000175 0.004330 0.001903 0.000172 0.000298 0.005428 0.002361
Bud Site 0.003731 0.001498 0.001708 0.006225 0.000268 0.001361 0.000947 0.004403 0.000448 0.001401 0.001236 0.005124
Cell Periphery 0.013783 0.003655 0.002843 0.067842 0.003590 0.004732 0.020511 0.035482 0.004120 0.004418 0.013798 0.048286
Cytoplasm 0.003457 0.013616 0.004442 0.006152 0.007622 0.005176 0.001726 0.010673 0.007406 0.007634 0.002758 0.008884
Cytoplasmic Foci 0.001973 0.002164 0.005435 0.001014 0.000820 0.001054 0.000328 0.001502 0.000880 0.001377 0.002269 0.001309
Eisosomes 0.000065 0.000011 0.000010 0.000319 0.000005 0.000009 0.000046 0.000099 0.000008 0.000009 0.000033 0.000186
Endoplasmic Reticulum 0.789513* 0.867297* 0.180842* 0.698911* 0.938381* 0.922190* 0.782953* 0.740238* 0.930646* 0.906205* 0.554194* 0.723886*
Endosome 0.017849 0.014986 0.036284 0.011283 0.002441 0.006925 0.014466 0.015619 0.003241 0.009272 0.022756 0.013903
Golgi 0.010222 0.001971 0.015701 0.012236 0.000511 0.001128 0.006852 0.011249 0.001016 0.001374 0.010214 0.011639
Lipid Particles 0.021686 0.005168 0.009303 0.074608 0.000564 0.002360 0.017811 0.064448 0.001661 0.003178 0.014579 0.068468
Mitochondria 0.000528 0.000519 0.000112 0.000579 0.000092 0.000256 0.000120 0.000621 0.000115 0.000333 0.000117 0.000604
Mitotic Spindle 0.005416 0.000868 0.000654 0.001211 0.000099 0.000142 0.000314 0.000452 0.000375 0.000353 0.000443 0.000752
None 0.000034 0.000605 0.000031 0.000679 0.000278 0.000577 0.000031 0.000855 0.000266 0.000585 0.000031 0.000785
Nuclear Periphery 0.002993 0.001587 0.018799 0.005498 0.000760 0.001285 0.006206 0.002725 0.000876 0.001373 0.010990 0.003822
Nuclear Periphery Foci 0.024371 0.020145 0.113257 0.011824 0.001605 0.004577 0.026874 0.015185 0.002788 0.009111 0.059693 0.013855
Nucleolus 0.000042 0.000075 0.000077 0.000027 0.000013 0.000019 0.000021 0.000033 0.000015 0.000035 0.000042 0.000031
Nucleus 0.000216 0.000818 0.000250 0.000537 0.000124 0.000167 0.000158 0.000434 0.000129 0.000357 0.000193 0.000475
Peroxisomes 0.000076 0.000064 0.000021 0.000086 0.000068 0.000025 0.000014 0.000069 0.000068 0.000037 0.000017 0.000076
Vacuole 0.093739 0.060226 0.547004* 0.092020 0.041079 0.043264 0.111650 0.088342 0.043815 0.048203 0.277053* 0.089797
Vacuole Periphery 0.008068 0.003951 0.055930 0.005294 0.001435 0.004492 0.004489 0.005161 0.001780 0.004335 0.024033 0.005214

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.22 2.03 -2.50 -2.56 -3.26 0.58 -0.01 -1.85 -3.17 -2.00 0.43 0.24 -2.43 -4.17 -3.45
Bud Neck 2.41 -2.87 -1.41 -3.40 2.05 -4.06 -2.18 -6.91 -6.54 1.18 -2.04 -3.82 -6.57 -6.21 2.06
Bud Site 0.91 0.71 -1.10 -2.99 -2.66 -2.08 -1.67 -5.88 -3.72 -4.21 -2.27 -2.34 -6.25 -4.63 -4.67
Cell Periphery 3.70 4.09 -11.79 -17.01 -17.42 -1.69 -3.21 -15.11 -14.24 -2.71 -0.51 -2.91 -22.15 -21.80 -9.06
Cytoplasm -3.13 -0.31 -1.53 2.24 -0.89 2.39 6.51 -2.54 -4.07 -6.48 -0.38 3.87 -1.97 -1.44 -5.02
Cytoplasmic Foci -0.18 -1.31 1.12 1.34 1.57 -0.51 1.34 -1.75 -1.20 -5.11 -1.10 -1.35 -1.26 0.14 1.07
Eisosomes 4.02 4.07 -5.04 -6.37 -6.38 -3.22 -5.46 -10.61 -10.12 -4.66 -1.02 -5.01 -8.95 -8.88 -7.51
Endoplasmic Reticulum -2.70 15.92 3.01 11.17 -18.48 2.94 7.11 22.77 19.13 1.98 4.44 15.53 28.18 22.69 -6.52
Endosome 0.75 -3.42 1.79 2.18 6.33 -7.71 -7.19 -17.81 -9.56 -0.58 -9.41 -10.27 -17.82 -5.81 4.45
Golgi 1.34 -0.73 -0.36 -6.67 0.71 -2.31 -3.61 -9.92 -9.20 -2.13 -0.97 -4.63 -11.40 -11.47 -0.72
Lipid Particles 2.58 1.78 -6.54 -12.53 -10.45 -2.53 -3.11 -16.91 -16.19 -6.76 -1.87 -3.48 -21.57 -20.65 -10.98
Mitochondria 0.01 1.47 -0.51 -0.52 -2.08 -2.71 -0.52 -3.67 -2.41 -2.99 -2.28 -0.22 -3.79 -1.99 -3.54
Mitotic Spindle 0.99 1.02 0.93 -0.39 -0.66 -0.36 -0.82 -2.13 -2.00 -0.49 0.15 -0.26 -1.04 -1.45 -0.84
None -2.38 0.14 -2.75 -0.38 -2.76 -1.12 1.89 -1.47 -0.65 -2.21 -1.49 1.90 -2.00 -0.75 -3.16
Nuclear Periphery 1.95 -4.43 -2.67 -9.23 3.85 -1.50 -4.14 -7.91 -4.15 2.64 -1.71 -6.40 -11.90 -8.56 4.52
Nuclear Periphery Foci 0.72 -4.97 1.87 1.98 6.32 -4.19 -4.04 -10.24 -7.43 1.73 -4.95 -7.49 -10.63 -3.47 5.89
Nucleolus -0.52 -1.13 0.38 0.77 1.76 -1.33 -0.71 -3.80 -2.83 -0.69 -1.24 -2.16 -3.89 0.10 0.99
Nucleus -1.75 -0.40 -1.58 0.29 -1.50 -1.04 -0.57 -4.12 -3.45 -2.90 -2.18 -1.28 -3.46 -1.35 -2.78
Peroxisomes 0.19 0.97 -0.21 -0.42 -1.67 2.41 2.92 -0.04 -5.02 -5.43 1.48 2.52 -0.36 -2.25 -3.89
Vacuole 1.88 -14.11 0.05 -3.99 16.45 -0.68 -4.63 -10.04 -8.62 1.31 -1.25 -12.33 -11.75 -9.66 9.63
Vacuole Periphery 1.12 -5.87 0.72 -1.46 6.91 -3.07 -2.94 -5.96 -0.62 -0.62 -3.34 -6.95 -6.55 -1.10 5.78
Standard name
Human Ortholog
Description Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves both isoprenylated and non-prenylated oligopeptides; contains multiple transmembrane spans; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and can complement yeast null mutant
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ste24

Ste24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available