Standard name | |
---|---|
Human Ortholog | |
Description | Highly conserved zinc metalloprotease; functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves both isoprenylated and non-prenylated oligopeptides; contains multiple transmembrane spans; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 6 | 2 | 2 | 4 | 10 | 5 | 3 | 5 | 8 | – | 2 | 2 | 0 | 1 | – | 0 | 0 | 0 | 0 | 2 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 8 | 2 | 6 | 6 | 6 | – | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 2 | 15 | 16 | 21 | 15 | 16 | 17 | 21 | – | 9 | 13 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | 23 | 66 | 83 | 191 | 222 | 313 | 378 | 246 | 285 | 267 | 261 | – | 40 | 62 | 4 | 21 | – | 3 | 4 | 1 | 3 | 4 | 4 |
Endoplasmic Reticulum | 31 | 17 | 272 | 168 | 132 | 126 | 173 | 182 | 103 | 117 | 114 | 86 | – | 130 | 114 | 2 | 2 | – | 95 | 56 | 354 | 121 | 277 | 249 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 2 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 5 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 7 | 0 | 1 | 2 | 3 | 4 | 4 | 4 | 1 | – | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 13 | 1 | 19 | 5 | 26 | 15 | 45 | 54 | 15 | 19 | 20 | 25 | – | 1 | 2 | 10 | 20 | – | 9 | 9 | 23 | 10 | 23 | 18 |
Unique Cell Count | 64 | 41 | 341 | 247 | 328 | 350 | 505 | 587 | 354 | 412 | 397 | 363 | 161 | 172 | 16 | 41 | 114 | 73 | 384 | 141 | 317 | 282 | ||
Labelled Cell Count | 67 | 42 | 368 | 273 | 355 | 383 | 558 | 660 | 390 | 455 | 436 | 411 | 183 | 193 | 19 | 45 | 114 | 73 | 384 | 141 | 317 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.5 | 20.8 | 24.8 | 23.7 | 22.6 | 19.7 | 18.2 | 17.5 | 16.0 | 14.4 | 14.3 | 15.3 | – | 19.5 | 19.6 | 19.0 | 20.5 | – | 23.7 | 25.6 | 27.8 |
Std Deviation (1e-4) | 5.1 | 4.9 | 3.9 | 4.1 | 4.5 | 3.5 | 3.2 | 3.2 | 3.2 | 2.8 | 2.9 | 3.4 | – | 4.1 | 3.7 | 4.3 | 6.3 | – | 4.7 | 5.2 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.13 | -0.33 | -0.45 | -0.5 | -0.63 | -0.78 | -0.8 | -0.7 | – | -0.35 | -0.34 | -0.38 | -0.27 | – | -0.06 | 0.05 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | – | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 3.5 | 2.9 | 3.2 | 3.5 | 3.3 | 3.5 | 4.2 | – | 0 | 0 | 0 | 0 | – |
Cytoplasm | 3.9 | 10.3 | 11.7 | 12.2 | 13.2 | 13.3 | 13.6 | 13.0 | 14.0 | – | 1.4 | 4.1 | 0 | 4.6 | – |
Endoplasmic Reticulum | -3.2 | -10.4 | -11.6 | -13.0 | -14.3 | -13.4 | -14.0 | -13.8 | -14.9 | – | 0.3 | -3.3 | -6.2 | -10.1 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – |
Vacuole | -2.1 | 1.2 | -0.8 | 1.8 | 2.0 | -0.8 | -0.6 | -0.3 | 0.7 | – | -2.6 | -2.4 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.4 | 23.4777 | 22.0707 | 20.2076 | 22.4162 | 22.5914 | 21.5695 | 27.8523 | 26.2269 | 24.9237 | 23.6328 | 25.612 | 22.7345 | 25.0574 | 22.645 | 19.0901 | 21.0587 | 25.2146 |
Actin | 0.0041 | 0.0001 | 0.0079 | 0.0002 | 0.0012 | 0.0001 | 0.0202 | 0 | 0.0097 | 0.0001 | 0.0318 | 0.0009 | 0.0091 | 0.0001 | 0.0185 | 0.0698 | 0.0138 | 0.0022 |
Bud | 0.0003 | 0.0001 | 0.003 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0001 |
Bud Neck | 0.0012 | 0.0006 | 0.0005 | 0.0035 | 0.0008 | 0.0107 | 0.0003 | 0.0003 | 0.0049 | 0.0003 | 0.0008 | 0.0002 | 0.0008 | 0.0008 | 0.0007 | 0.001 | 0.001 | 0.0004 |
Bud Periphery | 0.0006 | 0.0003 | 0.0097 | 0.0016 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0007 | 0.0017 | 0.0008 | 0.0004 |
Bud Site | 0.0017 | 0.0003 | 0.001 | 0.0057 | 0.0005 | 0.0001 | 0.0012 | 0.0001 | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0032 | 0.0016 | 0.0006 | 0.0001 |
Cell Periphery | 0.0091 | 0.0261 | 0.0163 | 0.0228 | 0.0069 | 0.0047 | 0.0083 | 0.027 | 0.0122 | 0.0106 | 0.0316 | 0.0061 | 0.0056 | 0.0092 | 0.0148 | 0.0398 | 0.0023 | 0.0072 |
Cytoplasm | 0.0301 | 0.06 | 0.0366 | 0.0239 | 0.0322 | 0.0361 | 0.0115 | 0.022 | 0.0107 | 0.0135 | 0.0132 | 0.0058 | 0.0084 | 0.0284 | 0.0102 | 0.007 | 0.0448 | 0.0088 |
Cytoplasmic Foci | 0.0067 | 0.001 | 0.0007 | 0.0005 | 0.001 | 0.0018 | 0.0084 | 0.0001 | 0.0036 | 0.0022 | 0.0026 | 0.0023 | 0.0099 | 0.0002 | 0.0092 | 0.0191 | 0.0021 | 0.0017 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0.0019 | 0.001 | 0 |
Endoplasmic Reticulum | 0.728 | 0.6632 | 0.7318 | 0.5576 | 0.645 | 0.7024 | 0.6903 | 0.7748 | 0.8081 | 0.7185 | 0.5181 | 0.7608 | 0.5352 | 0.4897 | 0.6028 | 0.1996 | 0.4436 | 0.6674 |
Endosome | 0.0104 | 0.0048 | 0.0043 | 0.0021 | 0.0106 | 0.0071 | 0.0249 | 0.0013 | 0.0052 | 0.0107 | 0.0224 | 0.0116 | 0.025 | 0.0029 | 0.016 | 0.0267 | 0.0264 | 0.009 |
Golgi | 0.0011 | 0.0019 | 0.0003 | 0.0001 | 0.002 | 0.0016 | 0.0044 | 0 | 0.0021 | 0.0004 | 0.006 | 0.003 | 0.031 | 0 | 0.0088 | 0.014 | 0.0049 | 0.0046 |
Lipid Particles | 0.0063 | 0.0085 | 0.0075 | 0.0022 | 0.0062 | 0.0035 | 0.0194 | 0.0064 | 0.0093 | 0.005 | 0.0116 | 0.0042 | 0.0074 | 0.0021 | 0.0289 | 0.0557 | 0.0099 | 0.0117 |
Mitochondria | 0.0013 | 0.002 | 0.0008 | 0.0001 | 0.0068 | 0.0002 | 0.0012 | 0 | 0.0097 | 0.0021 | 0.0011 | 0.0015 | 0.0024 | 0.0001 | 0.0263 | 0.003 | 0.0042 | 0.0031 |
None | 0.0043 | 0.0003 | 0.0082 | 0.0001 | 0.0022 | 0 | 0.0008 | 0 | 0.0021 | 0.0013 | 0.0003 | 0.0001 | 0.0445 | 0.0001 | 0.0009 | 0.021 | 0.0123 | 0.0001 |
Nuclear Periphery | 0.0525 | 0.0363 | 0.0389 | 0.0497 | 0.0627 | 0.0725 | 0.0833 | 0.0241 | 0.0325 | 0.0316 | 0.0876 | 0.0889 | 0.1616 | 0.0953 | 0.0492 | 0.0271 | 0.1886 | 0.1758 |
Nucleolus | 0.016 | 0.0112 | 0.0075 | 0.0909 | 0.0016 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.003 | 0.0009 | 0.0009 | 0.001 | 0.001 | 0.0001 |
Nucleus | 0.0206 | 0.009 | 0.0069 | 0.0493 | 0.0088 | 0.0109 | 0.0048 | 0.0014 | 0.001 | 0.0032 | 0.0098 | 0.004 | 0.0165 | 0.0297 | 0.0035 | 0.0028 | 0.0115 | 0.0043 |
Peroxisomes | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0072 | 0.0033 | 0 | 0.0021 | 0.0002 | 0.0033 | 0.0006 | 0.0014 | 0 | 0.0161 | 0.0236 | 0.0017 | 0.002 |
Punctate Nuclear | 0.0039 | 0.0004 | 0.0022 | 0.0007 | 0.0004 | 0.0001 | 0.0015 | 0 | 0.0004 | 0.0004 | 0.0005 | 0.0015 | 0.0117 | 0.0001 | 0.0005 | 0.0082 | 0.0037 | 0.0003 |
Vacuole | 0.0943 | 0.1702 | 0.1103 | 0.1847 | 0.1997 | 0.1333 | 0.1025 | 0.1396 | 0.0796 | 0.1866 | 0.2407 | 0.0977 | 0.1118 | 0.3162 | 0.1685 | 0.4574 | 0.2003 | 0.0817 |
Vacuole Periphery | 0.0065 | 0.0037 | 0.0053 | 0.0034 | 0.0105 | 0.0068 | 0.012 | 0.0025 | 0.0041 | 0.0119 | 0.0172 | 0.0102 | 0.0125 | 0.0234 | 0.0195 | 0.0177 | 0.0252 | 0.019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 102.8872 | 82.5601 | 84.3712 | 97.8574 | 86.656 | 77.7299 | 96.1078 | 100.0794 | 94.1621 | 106.2558 |
Translational Efficiency | 1.0435 | 1.1021 | 1.0483 | 0.9181 | 1.0949 | 1.3199 | 1.006 | 0.8854 | 1.0975 | 0.9902 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
130 | 523 | 106 | 914 | 2372 | 1273 | 173 | 1396 | 2502 | 1796 | 279 | 2310 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2077.97 | 1782.49 | 2004.34 | 2856.47 | 1625.72 | 1766.28 | 2342.27 | 2618.68 | 1649.22 | 1771.00 | 2213.88 | 2712.77 |
Standard Deviation | 389.46 | 345.38 | 243.84 | 618.43 | 256.52 | 320.19 | 385.14 | 506.86 | 283.44 | 327.81 | 376.13 | 565.78 |
Intensity Change Log 2 | — | -0.221281 | -0.052048 | 0.459059 | — | 0.119635 | 0.526829 | 0.687761 | — | -0.061644 | 0.230927 | 0.563935 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000202 | 0.000179 | 0.000077 | 0.000594 | 0.000175 | 0.000086 | 0.000152 | 0.000506 | 0.000176 | 0.000113 | 0.000123 | 0.000541 |
Bud Neck | 0.002035 | 0.000600 | 0.007221 | 0.003061 | 0.000070 | 0.000175 | 0.004330 | 0.001903 | 0.000172 | 0.000298 | 0.005428 | 0.002361 |
Bud Site | 0.003731 | 0.001498 | 0.001708 | 0.006225 | 0.000268 | 0.001361 | 0.000947 | 0.004403 | 0.000448 | 0.001401 | 0.001236 | 0.005124 |
Cell Periphery | 0.013783 | 0.003655 | 0.002843 | 0.067842 | 0.003590 | 0.004732 | 0.020511 | 0.035482 | 0.004120 | 0.004418 | 0.013798 | 0.048286 |
Cytoplasm | 0.003457 | 0.013616 | 0.004442 | 0.006152 | 0.007622 | 0.005176 | 0.001726 | 0.010673 | 0.007406 | 0.007634 | 0.002758 | 0.008884 |
Cytoplasmic Foci | 0.001973 | 0.002164 | 0.005435 | 0.001014 | 0.000820 | 0.001054 | 0.000328 | 0.001502 | 0.000880 | 0.001377 | 0.002269 | 0.001309 |
Eisosomes | 0.000065 | 0.000011 | 0.000010 | 0.000319 | 0.000005 | 0.000009 | 0.000046 | 0.000099 | 0.000008 | 0.000009 | 0.000033 | 0.000186 |
Endoplasmic Reticulum | 0.789513* | 0.867297* | 0.180842* | 0.698911* | 0.938381* | 0.922190* | 0.782953* | 0.740238* | 0.930646* | 0.906205* | 0.554194* | 0.723886* |
Endosome | 0.017849 | 0.014986 | 0.036284 | 0.011283 | 0.002441 | 0.006925 | 0.014466 | 0.015619 | 0.003241 | 0.009272 | 0.022756 | 0.013903 |
Golgi | 0.010222 | 0.001971 | 0.015701 | 0.012236 | 0.000511 | 0.001128 | 0.006852 | 0.011249 | 0.001016 | 0.001374 | 0.010214 | 0.011639 |
Lipid Particles | 0.021686 | 0.005168 | 0.009303 | 0.074608 | 0.000564 | 0.002360 | 0.017811 | 0.064448 | 0.001661 | 0.003178 | 0.014579 | 0.068468 |
Mitochondria | 0.000528 | 0.000519 | 0.000112 | 0.000579 | 0.000092 | 0.000256 | 0.000120 | 0.000621 | 0.000115 | 0.000333 | 0.000117 | 0.000604 |
Mitotic Spindle | 0.005416 | 0.000868 | 0.000654 | 0.001211 | 0.000099 | 0.000142 | 0.000314 | 0.000452 | 0.000375 | 0.000353 | 0.000443 | 0.000752 |
None | 0.000034 | 0.000605 | 0.000031 | 0.000679 | 0.000278 | 0.000577 | 0.000031 | 0.000855 | 0.000266 | 0.000585 | 0.000031 | 0.000785 |
Nuclear Periphery | 0.002993 | 0.001587 | 0.018799 | 0.005498 | 0.000760 | 0.001285 | 0.006206 | 0.002725 | 0.000876 | 0.001373 | 0.010990 | 0.003822 |
Nuclear Periphery Foci | 0.024371 | 0.020145 | 0.113257 | 0.011824 | 0.001605 | 0.004577 | 0.026874 | 0.015185 | 0.002788 | 0.009111 | 0.059693 | 0.013855 |
Nucleolus | 0.000042 | 0.000075 | 0.000077 | 0.000027 | 0.000013 | 0.000019 | 0.000021 | 0.000033 | 0.000015 | 0.000035 | 0.000042 | 0.000031 |
Nucleus | 0.000216 | 0.000818 | 0.000250 | 0.000537 | 0.000124 | 0.000167 | 0.000158 | 0.000434 | 0.000129 | 0.000357 | 0.000193 | 0.000475 |
Peroxisomes | 0.000076 | 0.000064 | 0.000021 | 0.000086 | 0.000068 | 0.000025 | 0.000014 | 0.000069 | 0.000068 | 0.000037 | 0.000017 | 0.000076 |
Vacuole | 0.093739 | 0.060226 | 0.547004* | 0.092020 | 0.041079 | 0.043264 | 0.111650 | 0.088342 | 0.043815 | 0.048203 | 0.277053* | 0.089797 |
Vacuole Periphery | 0.008068 | 0.003951 | 0.055930 | 0.005294 | 0.001435 | 0.004492 | 0.004489 | 0.005161 | 0.001780 | 0.004335 | 0.024033 | 0.005214 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.22 | 2.03 | -2.50 | -2.56 | -3.26 | 0.58 | -0.01 | -1.85 | -3.17 | -2.00 | 0.43 | 0.24 | -2.43 | -4.17 | -3.45 |
Bud Neck | 2.41 | -2.87 | -1.41 | -3.40 | 2.05 | -4.06 | -2.18 | -6.91 | -6.54 | 1.18 | -2.04 | -3.82 | -6.57 | -6.21 | 2.06 |
Bud Site | 0.91 | 0.71 | -1.10 | -2.99 | -2.66 | -2.08 | -1.67 | -5.88 | -3.72 | -4.21 | -2.27 | -2.34 | -6.25 | -4.63 | -4.67 |
Cell Periphery | 3.70 | 4.09 | -11.79 | -17.01 | -17.42 | -1.69 | -3.21 | -15.11 | -14.24 | -2.71 | -0.51 | -2.91 | -22.15 | -21.80 | -9.06 |
Cytoplasm | -3.13 | -0.31 | -1.53 | 2.24 | -0.89 | 2.39 | 6.51 | -2.54 | -4.07 | -6.48 | -0.38 | 3.87 | -1.97 | -1.44 | -5.02 |
Cytoplasmic Foci | -0.18 | -1.31 | 1.12 | 1.34 | 1.57 | -0.51 | 1.34 | -1.75 | -1.20 | -5.11 | -1.10 | -1.35 | -1.26 | 0.14 | 1.07 |
Eisosomes | 4.02 | 4.07 | -5.04 | -6.37 | -6.38 | -3.22 | -5.46 | -10.61 | -10.12 | -4.66 | -1.02 | -5.01 | -8.95 | -8.88 | -7.51 |
Endoplasmic Reticulum | -2.70 | 15.92 | 3.01 | 11.17 | -18.48 | 2.94 | 7.11 | 22.77 | 19.13 | 1.98 | 4.44 | 15.53 | 28.18 | 22.69 | -6.52 |
Endosome | 0.75 | -3.42 | 1.79 | 2.18 | 6.33 | -7.71 | -7.19 | -17.81 | -9.56 | -0.58 | -9.41 | -10.27 | -17.82 | -5.81 | 4.45 |
Golgi | 1.34 | -0.73 | -0.36 | -6.67 | 0.71 | -2.31 | -3.61 | -9.92 | -9.20 | -2.13 | -0.97 | -4.63 | -11.40 | -11.47 | -0.72 |
Lipid Particles | 2.58 | 1.78 | -6.54 | -12.53 | -10.45 | -2.53 | -3.11 | -16.91 | -16.19 | -6.76 | -1.87 | -3.48 | -21.57 | -20.65 | -10.98 |
Mitochondria | 0.01 | 1.47 | -0.51 | -0.52 | -2.08 | -2.71 | -0.52 | -3.67 | -2.41 | -2.99 | -2.28 | -0.22 | -3.79 | -1.99 | -3.54 |
Mitotic Spindle | 0.99 | 1.02 | 0.93 | -0.39 | -0.66 | -0.36 | -0.82 | -2.13 | -2.00 | -0.49 | 0.15 | -0.26 | -1.04 | -1.45 | -0.84 |
None | -2.38 | 0.14 | -2.75 | -0.38 | -2.76 | -1.12 | 1.89 | -1.47 | -0.65 | -2.21 | -1.49 | 1.90 | -2.00 | -0.75 | -3.16 |
Nuclear Periphery | 1.95 | -4.43 | -2.67 | -9.23 | 3.85 | -1.50 | -4.14 | -7.91 | -4.15 | 2.64 | -1.71 | -6.40 | -11.90 | -8.56 | 4.52 |
Nuclear Periphery Foci | 0.72 | -4.97 | 1.87 | 1.98 | 6.32 | -4.19 | -4.04 | -10.24 | -7.43 | 1.73 | -4.95 | -7.49 | -10.63 | -3.47 | 5.89 |
Nucleolus | -0.52 | -1.13 | 0.38 | 0.77 | 1.76 | -1.33 | -0.71 | -3.80 | -2.83 | -0.69 | -1.24 | -2.16 | -3.89 | 0.10 | 0.99 |
Nucleus | -1.75 | -0.40 | -1.58 | 0.29 | -1.50 | -1.04 | -0.57 | -4.12 | -3.45 | -2.90 | -2.18 | -1.28 | -3.46 | -1.35 | -2.78 |
Peroxisomes | 0.19 | 0.97 | -0.21 | -0.42 | -1.67 | 2.41 | 2.92 | -0.04 | -5.02 | -5.43 | 1.48 | 2.52 | -0.36 | -2.25 | -3.89 |
Vacuole | 1.88 | -14.11 | 0.05 | -3.99 | 16.45 | -0.68 | -4.63 | -10.04 | -8.62 | 1.31 | -1.25 | -12.33 | -11.75 | -9.66 | 9.63 |
Vacuole Periphery | 1.12 | -5.87 | 0.72 | -1.46 | 6.91 | -3.07 | -2.94 | -5.96 | -0.62 | -0.62 | -3.34 | -6.95 | -6.55 | -1.10 | 5.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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