Standard name | |
---|---|
Human Ortholog | |
Description | Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 2 | 17 | 11 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 4 | 2 | 1 | 4 | 6 | 20 | 19 | 15 | 26 | 25 | 26 | 2 | 1 | 2 | 4 | 10 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 600 | 472 | 242 | 192 | 250 | 243 | 258 | 247 | 213 | 249 | 133 | 215 | 251 | 290 | 328 | 512 | 320 | 255 | 563 | 481 | 217 | 150 | 198 | 187 |
Endoplasmic Reticulum | 5 | 6 | 1 | 4 | 9 | 8 | 29 | 43 | 36 | 31 | 23 | 25 | 2 | 9 | 6 | 33 | 22 | 22 | 8 | 2 | 8 | 6 | 5 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 4 | 5 | 4 | 5 | 4 | 0 | 0 | 2 | 10 | 4 | 0 | 0 | 0 | 2 | 0 | 3 | 5 |
Mitochondria | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 9 | 6 | 1 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 3 | 6 | 0 | 1 | 4 | 1 | 0 | 0 | 3 |
Unique Cell Count | 607 | 480 | 243 | 198 | 261 | 251 | 294 | 302 | 271 | 302 | 178 | 276 | 255 | 300 | 337 | 529 | 332 | 255 | 587 | 498 | 240 | 163 | 214 | 223 |
Labelled Cell Count | 609 | 490 | 246 | 200 | 265 | 257 | 313 | 318 | 295 | 327 | 199 | 313 | 255 | 301 | 338 | 564 | 362 | 281 | 587 | 498 | 240 | 163 | 214 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 134.2 | 153.2 | 132.9 | 135.4 | 119.8 | 84.9 | 73.5 | 60.5 | 53.8 | 47.4 | 46.1 | 40.1 | 161.5 | 149.1 | 136.2 | 116.9 | 137.5 | 137.9 | 132.3 | 121.8 | 113.0 |
Std Deviation (1e-4) | 26.9 | 31.9 | 31.8 | 29.7 | 22.7 | 19.7 | 15.8 | 13.0 | 11.9 | 11.2 | 10.4 | 9.5 | 31.6 | 33.9 | 33.3 | 40.9 | 41.1 | 40.9 | 35.8 | 35.0 | 35.8 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.15 | -0.65 | -0.85 | -1.14 | -1.31 | -1.49 | -1.53 | -1.73 | 0.28 | 0.17 | 0.04 | -0.18 | 0.05 | 0.05 | -0.01 | -0.13 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.5 | 3.3 | 3.0 | 4.1 | 5.5 | 4.3 | 0 | 0 | 0 | 0 | 1.8 | 0 |
Cytoplasm | -2.2 | -2.8 | -2.3 | -5.4 | -6.8 | -7.5 | -6.7 | -8.1 | -7.6 | -1.3 | -2.4 | -2.1 | -2.4 | -2.6 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 4.7 | 5.9 | 5.6 | 4.9 | 5.5 | 4.5 | 0 | 0 | 0 | 3.7 | 3.8 | 4.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 141.6779 | 197.4818 | 180.0105 | 178.2633 | 115.0663 | 148.3772 | 167.1036 | 229.316 | 194.3809 | 177.9171 | 175.6726 | 205.0407 | 157.0559 | 236.9239 | 209.9824 | 203.3693 | 148.4067 | 197.8512 |
Actin | 0.0124 | 0.0004 | 0.0019 | 0.0012 | 0.0105 | 0.0007 | 0.0067 | 0.0003 | 0.0044 | 0.0036 | 0.0017 | 0.0011 | 0.0067 | 0.0004 | 0.0004 | 0.0003 | 0.1987 | 0.0034 |
Bud | 0.0037 | 0.0012 | 0.0032 | 0.0026 | 0.0011 | 0.0055 | 0.0023 | 0.0009 | 0.0047 | 0.0028 | 0.0023 | 0.0018 | 0.0026 | 0.0008 | 0.0022 | 0.0015 | 0.0026 | 0.0019 |
Bud Neck | 0.0036 | 0.0012 | 0.002 | 0.0011 | 0.0009 | 0.0017 | 0.0009 | 0.001 | 0.001 | 0.0008 | 0.001 | 0.0044 | 0.0009 | 0.0006 | 0.0006 | 0.0062 | 0.0009 | 0.0023 |
Bud Periphery | 0.0053 | 0.0013 | 0.0027 | 0.0014 | 0.0011 | 0.003 | 0.0045 | 0.0007 | 0.0052 | 0.0032 | 0.0024 | 0.002 | 0.0019 | 0.0004 | 0.0008 | 0.0005 | 0.0029 | 0.0011 |
Bud Site | 0.0085 | 0.0013 | 0.0047 | 0.0006 | 0.0015 | 0.0011 | 0.0016 | 0.0019 | 0.0013 | 0.0018 | 0.0005 | 0.0003 | 0.0027 | 0.0008 | 0.0012 | 0.0002 | 0.0031 | 0.0002 |
Cell Periphery | 0.0743 | 0.0564 | 0.056 | 0.0414 | 0.009 | 0.0059 | 0.0495 | 0.0259 | 0.0192 | 0.0502 | 0.0086 | 0.0131 | 0.0524 | 0.018 | 0.0261 | 0.0022 | 0.001 | 0.0025 |
Cytoplasm | 0.4143 | 0.7194 | 0.5572 | 0.3508 | 0.3307 | 0.6003 | 0.6582 | 0.881 | 0.7682 | 0.5704 | 0.7767 | 0.8039 | 0.5818 | 0.9213 | 0.8185 | 0.7888 | 0.614 | 0.9378 |
Cytoplasmic Foci | 0.0123 | 0.0005 | 0.0075 | 0.0033 | 0.0962 | 0.0118 | 0.0101 | 0.0039 | 0.0125 | 0.0214 | 0.0113 | 0.0031 | 0.0783 | 0.0005 | 0.001 | 0.0023 | 0.011 | 0.0038 |
Eisosomes | 0.0021 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0019 | 0.0007 | 0.0004 | 0.0001 | 0 | 0 | 0.0038 | 0 | 0 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0276 | 0.0088 | 0.0044 | 0.003 | 0.0082 | 0.0026 | 0.0482 | 0.0051 | 0.014 | 0.0043 | 0.0067 | 0.0328 | 0.0116 | 0.0034 | 0.001 | 0.0013 | 0.0056 | 0.0054 |
Endosome | 0.0054 | 0.0013 | 0.0034 | 0.0103 | 0.0072 | 0.0067 | 0.0091 | 0.0009 | 0.0136 | 0.0221 | 0.0308 | 0.0076 | 0.0036 | 0.0006 | 0.0018 | 0.0081 | 0.0165 | 0.0031 |
Golgi | 0.0017 | 0.0001 | 0.0013 | 0.0048 | 0.0072 | 0.0004 | 0.0026 | 0.0001 | 0.0017 | 0.009 | 0.0014 | 0.0007 | 0.0027 | 0.0001 | 0.0002 | 0.0001 | 0.0692 | 0.0006 |
Lipid Particles | 0.0071 | 0.001 | 0.0025 | 0.0007 | 0.0168 | 0.0008 | 0.005 | 0.0013 | 0.004 | 0.0059 | 0.0026 | 0.0009 | 0.0114 | 0.0006 | 0.0013 | 0.0001 | 0.0033 | 0.0006 |
Mitochondria | 0.0233 | 0.0005 | 0.0095 | 0.0027 | 0.0259 | 0.0132 | 0.0073 | 0.001 | 0.0066 | 0.0286 | 0.001 | 0.005 | 0.0347 | 0.0002 | 0.0004 | 0.0002 | 0.0256 | 0.0017 |
None | 0.0087 | 0.0002 | 0.0023 | 0.0006 | 0.0175 | 0.0175 | 0.01 | 0.0008 | 0.0053 | 0.0052 | 0.0074 | 0.0043 | 0.0328 | 0.0004 | 0.0006 | 0.0002 | 0.0009 | 0.001 |
Nuclear Periphery | 0.0501 | 0.0112 | 0.0122 | 0.016 | 0.023 | 0.0084 | 0.0201 | 0.0034 | 0.0094 | 0.0147 | 0.0098 | 0.0174 | 0.0107 | 0.0048 | 0.0055 | 0.0019 | 0.0134 | 0.0066 |
Nucleolus | 0.0155 | 0.0005 | 0.0076 | 0.0021 | 0.0782 | 0.0543 | 0.0071 | 0.0019 | 0.0033 | 0.0025 | 0.0009 | 0.0047 | 0.0418 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0005 |
Nucleus | 0.019 | 0.0053 | 0.0109 | 0.0129 | 0.0167 | 0.0281 | 0.0109 | 0.0033 | 0.0065 | 0.0114 | 0.0067 | 0.0199 | 0.0113 | 0.0029 | 0.0078 | 0.0033 | 0.0068 | 0.0057 |
Peroxisomes | 0.0033 | 0 | 0.002 | 0.0002 | 0.0273 | 0.0006 | 0.0005 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0028 | 0 | 0.0001 | 0.0001 | 0.0056 | 0.0002 |
Punctate Nuclear | 0.0044 | 0 | 0.0003 | 0.0001 | 0.027 | 0.001 | 0.0007 | 0.0001 | 0.0005 | 0.0007 | 0.0012 | 0.0002 | 0.0063 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 |
Vacuole | 0.2348 | 0.1666 | 0.2529 | 0.3976 | 0.1947 | 0.1671 | 0.1265 | 0.0623 | 0.1033 | 0.2058 | 0.1057 | 0.0609 | 0.0881 | 0.0418 | 0.1106 | 0.1386 | 0.0137 | 0.0157 |
Vacuole Periphery | 0.0624 | 0.0225 | 0.0551 | 0.1468 | 0.0988 | 0.0692 | 0.0163 | 0.0034 | 0.0144 | 0.0347 | 0.0213 | 0.0159 | 0.0112 | 0.0022 | 0.0183 | 0.0441 | 0.0038 | 0.0058 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1762.8645 | 1951.2575 | 2140.8617 | 1740.5958 | 1816.2654 | 1656.4156 | 1673.4757 | 1381.8378 | 1461.4749 | 1255.9642 |
Translational Efficiency | 1.1824 | 1.2211 | 1.1678 | 1.4613 | 1.2893 | 1.2056 | 1.2487 | 1.6007 | 1.2427 | 1.386 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
101 | 1267 | 335 | 754 | 1992 | 254 | 578 | 119 | 2093 | 1521 | 913 | 873 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 8889.84 | 9752.16 | 14409.00 | 13099.53 | 8746.80 | 9888.49 | 13052.82 | 12536.44 | 8753.70 | 9774.93 | 13550.43 | 13022.77 |
Standard Deviation | 1506.53 | 2450.97 | 2919.75 | 4019.48 | 1854.65 | 2310.30 | 2809.10 | 3525.93 | 1839.62 | 2428.58 | 2924.18 | 3960.55 |
Intensity Change Log 2 | — | 0.133564 | 0.696741 | 0.559286 | — | 0.176995 | 0.577534 | 0.519301 | — | 0.155267 | 0.638852 | 0.539594 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000118 | 0.000114 | 0.000194 | 0.000969 | 0.000052 | 0.000205 | 0.000200 | 0.000277 | 0.000055 | 0.000129 | 0.000197 | 0.000875 |
Bud Neck | 0.001803 | 0.000713 | 0.005561 | 0.002523 | 0.000376 | 0.002435 | 0.004368 | 0.005888 | 0.000445 | 0.001001 | 0.004806 | 0.002982 |
Bud Site | 0.001534 | 0.003769 | 0.003864 | 0.015179 | 0.001838 | 0.008105 | 0.004842 | 0.010113 | 0.001823 | 0.004493 | 0.004483 | 0.014489 |
Cell Periphery | 0.061188 | 0.025029 | 0.075637 | 0.070775 | 0.009666 | 0.038631 | 0.045964 | 0.035593 | 0.012152 | 0.027300 | 0.056852 | 0.065979 |
Cytoplasm | 0.290691* | 0.702656* | 0.216058* | 0.422898* | 0.803664* | 0.590337* | 0.347839* | 0.243430* | 0.778910* | 0.683899* | 0.299485* | 0.398434* |
Cytoplasmic Foci | 0.031920 | 0.009226 | 0.023694 | 0.009380 | 0.007097 | 0.022741 | 0.009742 | 0.017428 | 0.008295 | 0.011483 | 0.014861 | 0.010477 |
Eisosomes | 0.000007 | 0.000003 | 0.000023 | 0.000013 | 0.000001 | 0.000011 | 0.000010 | 0.000014 | 0.000001 | 0.000005 | 0.000015 | 0.000013 |
Endoplasmic Reticulum | 0.000266 | 0.000646 | 0.000375 | 0.000406 | 0.000578 | 0.000346 | 0.000227 | 0.000196 | 0.000563 | 0.000596 | 0.000281 | 0.000377 |
Endosome | 0.000752 | 0.000528 | 0.000819 | 0.001604 | 0.000447 | 0.000933 | 0.000675 | 0.001645 | 0.000462 | 0.000595 | 0.000728 | 0.001609 |
Golgi | 0.095490 | 0.054121 | 0.052553 | 0.019278 | 0.035103 | 0.059711 | 0.025921 | 0.016870 | 0.038017 | 0.055054 | 0.035693 | 0.018950 |
Lipid Particles | 0.053744 | 0.017212 | 0.040063 | 0.039086 | 0.010670 | 0.028268 | 0.021128 | 0.023248 | 0.012749 | 0.019059 | 0.028075 | 0.036927 |
Mitochondria | 0.000092 | 0.000249 | 0.000161 | 0.001748 | 0.000131 | 0.000855 | 0.000166 | 0.000293 | 0.000129 | 0.000350 | 0.000164 | 0.001550 |
Mitotic Spindle | 0.000623 | 0.000927 | 0.002069 | 0.001059 | 0.000782 | 0.002563 | 0.001636 | 0.000639 | 0.000774 | 0.001200 | 0.001795 | 0.001002 |
None | 0.001754 | 0.003232 | 0.001154 | 0.002238 | 0.001744 | 0.003009 | 0.001836 | 0.000637 | 0.001745 | 0.003195 | 0.001585 | 0.002020 |
Nuclear Periphery | 0.002522 | 0.000859 | 0.004716 | 0.000709 | 0.000580 | 0.001563 | 0.002109 | 0.000797 | 0.000674 | 0.000977 | 0.003066 | 0.000721 |
Nuclear Periphery Foci | 0.026091 | 0.012037 | 0.016004 | 0.005584 | 0.011045 | 0.017017 | 0.010776 | 0.006714 | 0.011771 | 0.012869 | 0.012694 | 0.005738 |
Nucleolus | 0.000097 | 0.000103 | 0.000160 | 0.000278 | 0.000054 | 0.001534 | 0.000211 | 0.000484 | 0.000056 | 0.000342 | 0.000192 | 0.000306 |
Nucleus | 0.023939 | 0.012564 | 0.019013 | 0.012328 | 0.009740 | 0.024065 | 0.022526 | 0.019466 | 0.010425 | 0.014485 | 0.021237 | 0.013301 |
Peroxisomes | 0.000008 | 0.000030 | 0.000021 | 0.000287 | 0.000013 | 0.000875 | 0.000023 | 0.000066 | 0.000012 | 0.000171 | 0.000022 | 0.000256 |
Vacuole | 0.319183* | 0.090323 | 0.433877* | 0.351695* | 0.060332 | 0.155357 | 0.443896* | 0.556551* | 0.072823 | 0.101183 | 0.440220* | 0.379619* |
Vacuole Periphery | 0.088179 | 0.065659 | 0.103985* | 0.041964 | 0.046087 | 0.041437 | 0.055904 | 0.059652 | 0.048118 | 0.061614 | 0.073546 | 0.044375 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.12 | -3.86 | -2.32 | -2.31 | -2.08 | -3.32 | -7.45 | -3.11 | -1.13 | -1.23 | -4.69 | -9.14 | -2.59 | -2.35 | -2.09 |
Bud Neck | 3.38 | -6.84 | -2.33 | -5.48 | 4.57 | -5.14 | -9.89 | -5.47 | -3.80 | -1.23 | -5.95 | -13.40 | -8.00 | -6.42 | 4.34 |
Bud Site | -4.89 | -4.41 | -7.70 | -6.24 | -6.16 | -2.73 | -5.69 | -4.38 | -0.93 | -2.28 | -4.91 | -6.55 | -8.15 | -6.17 | -5.90 |
Cell Periphery | 4.74 | -1.26 | -0.67 | -9.63 | 0.88 | -7.44 | -8.10 | -2.78 | 1.67 | 1.62 | -9.57 | -12.27 | -13.43 | -8.68 | -2.15 |
Cytoplasm | -12.52 | 2.47 | -3.57 | 17.94 | -9.98 | 9.51 | 29.06 | 19.74 | 9.08 | 2.93 | 8.91 | 37.20 | 28.16 | 19.45 | -6.07 |
Cytoplasmic Foci | 3.60 | 0.57 | 3.10 | -1.11 | 4.33 | -6.09 | -4.64 | -2.71 | -0.09 | -1.79 | -3.57 | -6.64 | -2.49 | -0.78 | 2.15 |
Eisosomes | 2.19 | -5.63 | -2.84 | -3.99 | 2.82 | -2.67 | -10.56 | -3.02 | -0.78 | -1.19 | -3.45 | -11.42 | -6.01 | -3.81 | 0.75 |
Endoplasmic Reticulum | -3.84 | -1.16 | -2.09 | 2.75 | -0.79 | 1.79 | 3.43 | 3.82 | 2.38 | 1.53 | -0.21 | 2.98 | 2.01 | 3.02 | -2.60 |
Endosome | 2.22 | -0.71 | -1.68 | -2.20 | -1.53 | -3.06 | -4.35 | -2.27 | -0.97 | -1.58 | -2.41 | -5.46 | -2.64 | -2.29 | -1.86 |
Golgi | 3.61 | 3.94 | 7.16 | 12.58 | 8.67 | -4.30 | 5.66 | 9.40 | 8.18 | 4.02 | -6.13 | 2.35 | 10.32 | 14.55 | 7.45 |
Lipid Particles | 4.78 | 1.65 | 1.92 | -6.82 | 0.55 | -5.20 | -5.44 | -3.51 | 1.68 | -0.37 | -4.06 | -8.71 | -9.04 | -6.08 | -2.92 |
Mitochondria | -4.70 | -3.35 | -2.43 | -2.21 | -2.32 | -1.46 | -3.57 | -1.33 | 0.98 | -0.79 | -2.56 | -3.82 | -2.43 | -2.04 | -2.31 |
Mitotic Spindle | -2.66 | -5.76 | -1.12 | -0.28 | 2.43 | -3.17 | -2.55 | 0.20 | 3.13 | 2.52 | -2.89 | -3.76 | -0.66 | 0.67 | 2.45 |
None | -3.46 | 2.19 | -0.97 | 1.16 | -2.04 | -3.53 | 0.16 | 5.52 | 5.81 | 1.33 | -4.98 | 0.67 | -0.83 | 1.69 | -1.09 |
Nuclear Periphery | 5.92 | -5.08 | 6.30 | 1.32 | 10.40 | -5.26 | -7.03 | -1.91 | 3.07 | 5.02 | -4.06 | -11.85 | -0.79 | 2.48 | 11.09 |
Nuclear Periphery Foci | 4.68 | 3.20 | 7.22 | 7.32 | 8.48 | -3.10 | 0.45 | 3.55 | 4.99 | 2.86 | -1.08 | -0.76 | 8.26 | 8.80 | 8.62 |
Nucleolus | -0.20 | -5.08 | -3.25 | -3.12 | -2.26 | -1.32 | -6.93 | -2.46 | 1.11 | -1.48 | -1.42 | -8.73 | -4.49 | 0.63 | -2.09 |
Nucleus | 3.05 | 0.39 | 2.21 | -1.10 | 2.21 | -4.37 | -7.09 | -3.57 | -0.59 | 0.94 | -3.31 | -7.84 | -3.30 | -1.05 | 4.44 |
Peroxisomes | -5.32 | -5.21 | -2.49 | -2.28 | -2.35 | -1.12 | -3.93 | -3.05 | 1.05 | -2.43 | -1.23 | -4.86 | -2.54 | -0.34 | -2.41 |
Vacuole | 8.62 | -4.02 | -1.44 | -23.29 | 4.38 | -6.98 | -28.65 | -17.13 | -12.27 | -3.41 | -5.39 | -35.30 | -29.13 | -25.43 | 3.92 |
Vacuole Periphery | 1.29 | -0.88 | 3.17 | 6.12 | 8.45 | 1.10 | -0.43 | 0.34 | -0.33 | 0.54 | -3.70 | -4.58 | 2.29 | 5.58 | 6.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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