Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 1 | 5 | 8 | 3 | 9 | 6 | 8 | 10 | 12 | 13 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 4 |
Bud Neck | 3 | 1 | 26 | 22 | 34 | 28 | 51 | 46 | 24 | 34 | 36 | 37 | 14 | 11 | 13 | 3 | 5 | 1 | 2 | 2 | 5 | 1 | 7 | 11 |
Bud Site | 2 | 2 | 2 | 4 | 10 | 19 | 17 | 21 | 15 | 26 | 24 | 19 | 3 | 1 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 3 | 8 | 6 | 13 | 4 | 11 | 11 | 3 | 7 | 7 | 9 | 21 | 30 | 38 | 15 | 12 | 12 | 1 | 0 | 1 | 0 | 1 | 1 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 2 | 3 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 9 | 7 | 13 | 10 | 18 | 9 | 18 | 23 | 10 | 20 | 19 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 4 |
Mitochondria | 13 | 40 | 7 | 8 | 1 | 22 | 29 | 11 | 41 | 34 | 44 | 25 | 13 | 3 | 3 | 5 | 3 | 7 | 1 | 3 | 1 | 0 | 2 | 4 |
Nucleus | 31 | 64 | 64 | 26 | 27 | 25 | 32 | 40 | 30 | 22 | 17 | 25 | 24 | 13 | 24 | 20 | 18 | 19 | 8 | 26 | 10 | 4 | 6 | 4 |
Nuclear Periphery | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 |
Nucleolus | 84 | 119 | 88 | 78 | 80 | 108 | 115 | 135 | 131 | 143 | 112 | 124 | 160 | 119 | 120 | 148 | 101 | 92 | 62 | 122 | 107 | 37 | 40 | 51 |
Peroxisomes | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 7 | 4 | 12 | 1 | 10 | 2 | 1 | 1 | 1 | 1 | 3 | 4 |
SpindlePole | 50 | 86 | 186 | 147 | 218 | 196 | 239 | 265 | 201 | 208 | 159 | 214 | 122 | 131 | 117 | 137 | 104 | 92 | 50 | 71 | 113 | 107 | 118 | 123 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 5 | 8 | 1 | 2 | 5 | 3 | 5 | 5 |
Unique Cell Count | 125 | 225 | 258 | 190 | 270 | 252 | 332 | 340 | 270 | 289 | 241 | 285 | 247 | 218 | 229 | 213 | 171 | 153 | 142 | 264 | 283 | 174 | 218 | 247 |
Labelled Cell Count | 190 | 322 | 389 | 297 | 396 | 408 | 505 | 538 | 453 | 485 | 412 | 467 | 374 | 324 | 336 | 355 | 273 | 253 | 142 | 264 | 283 | 174 | 218 | 247 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.1 | 5.5 | 5.0 | 5.5 | 4.2 | 4.4 | 4.7 | 3.9 | 3.7 | 3.5 | 4.1 | 5.1 | 5.6 | 5.1 | 8.9 | 9.6 | 8.7 | 5.2 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.0 | 1.3 | 1.4 | 0.7 | 1.0 | 1.1 | 0.8 | 0.7 | 0.8 | 1.0 | 1.1 | 1.5 | 1.4 | 1.9 | 2.6 | 2.3 | 1.6 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.0 | -0.38 | -0.32 | -0.22 | -0.51 | -0.59 | -0.67 | -0.43 | -0.12 | 0.0 | -0.11 | 0.69 | 0.79 | 0.65 | -0.1 | -0.03 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.5 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | -2.0 | -1.8 | -3.9 | -2.8 | -3.7 |
Bud Site | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.0 | -1.1 | 0.1 | 0.1 | -1.6 | -0.5 | -0.1 | 0 | 2.6 | 4.3 | 5.1 | 2.0 | 1.9 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.7 | 1.1 | 2.6 | 2.5 | 4.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 0 | -0.3 | 0 | 1.0 |
Nucleus | -2.9 | -4.5 | -4.4 | -5.0 | -4.2 | -4.1 | -5.5 | -5.4 | -5.0 | -4.5 | -5.6 | -4.1 | -4.4 | -3.7 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.5 | -1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.9 | 4.5 | 4.1 | 7.6 | 5.1 | 5.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 |
SpindlePole | 1.3 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.2 | -2.8 | -4.8 | -1.8 | -2.4 | -2.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1017 | 0.3342 | 0.2302 | 0.2358 | -0.3234 | 0.4562 | 1.3231 | 1.4171 | 1.3757 | 1.5035 | 1.1863 | 1.0768 | 0.8522 | 0.7876 | 0.9153 | 1.5796 | 0.5098 | 0.6434 |
Actin | 0.0493 | 0 | 0.0197 | 0.0004 | 0.0001 | 0.0235 | 0.0081 | 0 | 0.0027 | 0.0002 | 0 | 0.0007 | 0.016 | 0 | 0.0189 | 0.0677 | 0 | 0.0013 |
Bud | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Bud Neck | 0.0053 | 0.0021 | 0.0017 | 0.0017 | 0.0147 | 0.0019 | 0.0023 | 0.0017 | 0.0015 | 0.0056 | 0.0025 | 0.0008 | 0.0021 | 0.0016 | 0.0017 | 0.0013 | 0.0025 | 0.0011 |
Bud Periphery | 0.0017 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | 0.003 | 0.0019 | 0.0022 | 0.0002 | 0.0018 | 0.0004 | 0.0009 | 0.0006 | 0.0013 | 0.0008 | 0.0003 | 0.0001 | 0.0021 | 0.0013 | 0.0008 | 0.0011 | 0.0002 | 0.0002 |
Cell Periphery | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 |
Cytoplasm | 0.0046 | 0.0002 | 0.001 | 0.0012 | 0.0028 | 0.0004 | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0032 | 0.0001 | 0.003 | 0.0011 | 0 | 0 |
Cytoplasmic Foci | 0.0508 | 0.0236 | 0.0226 | 0.0166 | 0.0097 | 0.0158 | 0.0355 | 0.0144 | 0.0205 | 0.0278 | 0.0128 | 0.0136 | 0.0242 | 0.0164 | 0.0099 | 0.0175 | 0.0033 | 0.0088 |
Eisosomes | 0.001 | 0 | 0.0005 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0007 | 0 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0001 | 0.0009 | 0.0002 | 0.0004 | 0.0024 | 0.0011 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0027 | 0 | 0.0038 | 0.0036 | 0 | 0.0002 |
Endosome | 0.0266 | 0.0052 | 0.004 | 0.0036 | 0.0053 | 0.0089 | 0.0156 | 0.0006 | 0.0041 | 0.0031 | 0.0006 | 0.0054 | 0.0127 | 0.0023 | 0.0064 | 0.0224 | 0.0002 | 0.0015 |
Golgi | 0.0085 | 0.0017 | 0.0059 | 0.0024 | 0.0014 | 0.0088 | 0.0082 | 0.0007 | 0.005 | 0.0036 | 0.0013 | 0.0067 | 0.0049 | 0.0013 | 0.005 | 0.0062 | 0.0005 | 0.0016 |
Lipid Particles | 0.0829 | 0.0756 | 0.065 | 0.1542 | 0.0436 | 0.0696 | 0.0928 | 0.0379 | 0.0693 | 0.1123 | 0.0708 | 0.0394 | 0.0773 | 0.0366 | 0.0367 | 0.0374 | 0.0065 | 0.0327 |
Mitochondria | 0.0056 | 0.0003 | 0.0022 | 0.0005 | 0.0009 | 0.022 | 0.0045 | 0.0001 | 0.0094 | 0.0007 | 0.0001 | 0.0028 | 0.019 | 0.0002 | 0.0009 | 0.0076 | 0.0001 | 0.0002 |
None | 0.0064 | 0.0002 | 0.013 | 0.0064 | 0.0016 | 0.0005 | 0.0016 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0074 | 0.0001 | 0.0021 | 0.0017 | 0 | 0.0001 |
Nuclear Periphery | 0.0145 | 0.0015 | 0.0096 | 0.0063 | 0.0039 | 0.006 | 0.0054 | 0.0002 | 0.0013 | 0.001 | 0.0019 | 0.0067 | 0.0099 | 0.0003 | 0.012 | 0.0164 | 0.001 | 0.0044 |
Nucleolus | 0.3333 | 0.5331 | 0.5951 | 0.3946 | 0.549 | 0.3378 | 0.4987 | 0.657 | 0.6209 | 0.6886 | 0.5114 | 0.399 | 0.4743 | 0.5701 | 0.6772 | 0.7063 | 0.4177 | 0.4126 |
Nucleus | 0.0222 | 0.0363 | 0.0483 | 0.1181 | 0.0721 | 0.0288 | 0.0228 | 0.0545 | 0.0263 | 0.0461 | 0.0196 | 0.0191 | 0.0319 | 0.0733 | 0.053 | 0.0476 | 0.0245 | 0.0309 |
Peroxisomes | 0.0167 | 0.0026 | 0.005 | 0.0024 | 0.0045 | 0.0055 | 0.0058 | 0.0012 | 0.0034 | 0.003 | 0.0014 | 0.0008 | 0.0081 | 0.002 | 0.0113 | 0.0066 | 0.0005 | 0.001 |
Punctate Nuclear | 0.3457 | 0.3021 | 0.1996 | 0.2899 | 0.2817 | 0.4664 | 0.2922 | 0.2302 | 0.2315 | 0.1046 | 0.376 | 0.5022 | 0.3006 | 0.2919 | 0.1556 | 0.0506 | 0.5426 | 0.5029 |
Vacuole | 0.0131 | 0.0117 | 0.0021 | 0.0006 | 0.0043 | 0.0004 | 0.0018 | 0.0005 | 0.0013 | 0.0013 | 0.0007 | 0.0008 | 0.0016 | 0.0019 | 0.0007 | 0.0017 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0026 | 0.001 | 0.0009 | 0.0003 | 0.0006 | 0.0004 | 0.0011 | 0.0001 | 0.0008 | 0.0004 | 0.0001 | 0.0012 | 0.0007 | 0.0002 | 0.0005 | 0.0015 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.005 | 14.0546 | 4.4245 | 5.5216 | 8.6479 | 7.6186 | 14.6412 | 10.7537 | 7.3581 | 13.0699 |
Translational Efficiency | 1.3409 | 1.1636 | 1.1427 | 1.2861 | 1.4062 | 1.3123 | 0.907 | 0.8326 | 1.0516 | 0.8279 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1795 | 155 | 459 | 1362 | 1490 | 1638 | 128 | 98 | 3285 | 1793 | 587 | 1460 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 694.97 | 842.58 | 1002.49 | 982.70 | 679.45 | 768.71 | 1018.05 | 1069.44 | 687.93 | 775.10 | 1005.88 | 988.52 |
Standard Deviation | 80.36 | 119.98 | 139.89 | 136.12 | 91.87 | 106.55 | 138.46 | 120.17 | 86.12 | 109.75 | 139.73 | 136.84 |
Intensity Change Log 2 | — | 0.277863 | 0.528565 | 0.499800 | — | 0.178072 | 0.583369 | 0.654416 | — | 0.229393 | 0.555918 | 0.578306 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000234 | 0.000751 | 0.000497 | 0.000449 | 0.000493 | 0.000545 | 0.000599 | 0.000475 | 0.000352 | 0.000563 | 0.000519 | 0.000451 |
Bud Neck | 0.010416 | 0.010371 | 0.006612 | 0.014860 | 0.006289 | 0.010052 | 0.008489 | 0.012714 | 0.008544 | 0.010080 | 0.007021 | 0.014716 |
Bud Site | 0.003798 | 0.002740 | 0.001942 | 0.005859 | 0.002368 | 0.002773 | 0.002790 | 0.004776 | 0.003149 | 0.002770 | 0.002127 | 0.005786 |
Cell Periphery | 0.000181 | 0.000096 | 0.000042 | 0.000071 | 0.000201 | 0.000170 | 0.000052 | 0.000061 | 0.000190 | 0.000163 | 0.000044 | 0.000071 |
Cytoplasm | 0.009899 | 0.002088 | 0.000149 | 0.000819 | 0.007906 | 0.004044 | 0.000227 | 0.000494 | 0.008995 | 0.003875 | 0.000166 | 0.000797 |
Cytoplasmic Foci | 0.016321 | 0.004539 | 0.000977 | 0.003443 | 0.019434 | 0.011876 | 0.001678 | 0.001983 | 0.017733 | 0.011242 | 0.001130 | 0.003345 |
Eisosomes | 0.000167 | 0.000163 | 0.000080 | 0.000068 | 0.000207 | 0.000176 | 0.000110 | 0.000073 | 0.000185 | 0.000175 | 0.000087 | 0.000068 |
Endoplasmic Reticulum | 0.002649 | 0.004495 | 0.001537 | 0.000934 | 0.004748 | 0.004413 | 0.002167 | 0.000918 | 0.003601 | 0.004421 | 0.001675 | 0.000933 |
Endosome | 0.010692 | 0.010021 | 0.000613 | 0.003474 | 0.010353 | 0.011424 | 0.000791 | 0.001091 | 0.010538 | 0.011302 | 0.000652 | 0.003314 |
Golgi | 0.009195 | 0.005261 | 0.001389 | 0.003775 | 0.012836 | 0.008572 | 0.001658 | 0.001401 | 0.010846 | 0.008285 | 0.001447 | 0.003616 |
Lipid Particles | 0.005580 | 0.006125 | 0.000275 | 0.000474 | 0.014904 | 0.009290 | 0.000423 | 0.000290 | 0.009809 | 0.009016 | 0.000307 | 0.000462 |
Mitochondria | 0.003483 | 0.003179 | 0.000898 | 0.003171 | 0.005159 | 0.010254 | 0.000627 | 0.001489 | 0.004243 | 0.009643 | 0.000839 | 0.003058 |
Mitotic Spindle | 0.081095 | 0.296540* | 0.279785* | 0.310237* | 0.096093 | 0.167032* | 0.275976* | 0.390571* | 0.087898 | 0.178228* | 0.278955* | 0.315630* |
None | 0.009381 | 0.003072 | 0.000434 | 0.000901 | 0.010667 | 0.004468 | 0.000465 | 0.000713 | 0.009965 | 0.004347 | 0.000441 | 0.000888 |
Nuclear Periphery | 0.000896 | 0.001849 | 0.001018 | 0.002967 | 0.001438 | 0.002250 | 0.001063 | 0.001055 | 0.001142 | 0.002216 | 0.001028 | 0.002838 |
Nuclear Periphery Foci | 0.088804 | 0.121829 | 0.011049 | 0.010154 | 0.180652 | 0.122374 | 0.017386 | 0.008506 | 0.130465 | 0.122327 | 0.012431 | 0.010044 |
Nucleolus | 0.611303* | 0.323145* | 0.507117* | 0.500492* | 0.481172* | 0.446542* | 0.501653* | 0.400718* | 0.552279* | 0.435875* | 0.505926* | 0.493795* |
Nucleus | 0.118848 | 0.180011* | 0.165001* | 0.115481 | 0.122178 | 0.163189* | 0.156905* | 0.145585* | 0.120359 | 0.164643* | 0.163235* | 0.117502 |
Peroxisomes | 0.015142 | 0.020991 | 0.018530 | 0.013902 | 0.020894 | 0.015301 | 0.024064 | 0.020689 | 0.017751 | 0.015793 | 0.019737 | 0.014358 |
Vacuole | 0.001346 | 0.001925 | 0.001583 | 0.005026 | 0.001365 | 0.003413 | 0.002265 | 0.004607 | 0.001355 | 0.003285 | 0.001731 | 0.004998 |
Vacuole Periphery | 0.000569 | 0.000810 | 0.000471 | 0.003440 | 0.000642 | 0.001841 | 0.000613 | 0.001790 | 0.000602 | 0.001752 | 0.000502 | 0.003330 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.20 | -6.86 | -4.70 | 1.96 | 1.95 | -1.83 | -2.68 | -1.62 | -0.60 | 0.96 | -6.25 | -6.26 | -3.16 | 1.00 | 2.27 |
Bud Neck | -0.86 | 0.74 | -9.03 | -4.39 | -9.22 | -5.02 | -3.23 | -3.95 | -2.98 | -2.29 | -2.55 | -2.15 | -11.99 | -9.28 | -9.13 |
Bud Site | -0.76 | 1.10 | -5.28 | -1.37 | -5.86 | -1.23 | -2.50 | -3.24 | -3.06 | -1.72 | 0.31 | -1.52 | -6.76 | -6.69 | -5.47 |
Cell Periphery | 3.75 | 6.93 | 5.04 | 1.12 | -5.20 | 1.62 | 9.97 | 6.52 | 4.92 | -1.50 | 1.36 | 11.51 | 8.53 | 6.64 | -5.24 |
Cytoplasm | 4.77 | 7.42 | 6.07 | 0.80 | -2.98 | 2.69 | 4.29 | 5.10 | 3.77 | -0.02 | 4.63 | 9.22 | 7.51 | 3.49 | -2.36 |
Cytoplasmic Foci | 8.27 | 11.54 | 9.09 | 0.09 | -6.05 | 4.04 | 9.43 | 7.54 | 4.60 | -0.85 | 5.33 | 15.57 | 12.38 | 6.08 | -5.54 |
Eisosomes | 0.53 | 14.80 | 24.57 | 7.70 | 3.55 | 5.51 | 11.06 | 16.54 | 12.42 | 3.58 | 2.60 | 20.32 | 35.26 | 23.44 | 5.15 |
Endoplasmic Reticulum | -4.96 | 3.50 | 13.74 | 8.91 | 4.88 | 0.93 | 8.11 | 16.32 | 15.25 | 4.54 | -5.60 | 9.21 | 23.15 | 21.54 | 6.28 |
Endosome | 0.29 | 9.96 | 7.08 | 1.30 | -4.35 | -0.47 | 7.16 | 8.53 | 8.71 | 0.15 | -0.42 | 13.30 | 9.49 | 7.40 | -3.89 |
Golgi | 4.36 | 12.21 | 6.91 | 2.69 | -2.21 | 3.86 | 12.26 | 12.26 | 9.30 | 0.62 | 3.45 | 17.35 | 9.65 | 5.71 | -2.15 |
Lipid Particles | -0.34 | 7.07 | 6.91 | 3.59 | -1.14 | 3.17 | 10.19 | 10.23 | 10.00 | 0.53 | 0.37 | 12.32 | 12.19 | 10.35 | -1.03 |
Mitochondria | 0.16 | 6.33 | 0.59 | 0.10 | -4.01 | -3.57 | 6.51 | 4.05 | 6.16 | -3.24 | -4.49 | 8.66 | 2.15 | 5.36 | -4.30 |
Mitotic Spindle | -7.16 | -12.35 | -21.66 | -1.05 | -1.72 | -8.08 | -6.33 | -7.70 | -5.85 | -2.51 | -11.44 | -13.79 | -23.30 | -12.58 | -2.34 |
None | 3.93 | 8.78 | 8.17 | 1.57 | -2.13 | 4.38 | 8.09 | 7.76 | 6.33 | -1.38 | 5.52 | 11.94 | 11.13 | 6.57 | -2.14 |
Nuclear Periphery | -3.66 | -1.89 | -3.48 | -1.58 | -2.87 | -6.05 | 1.06 | 0.60 | 3.75 | -0.15 | -8.79 | -0.41 | -3.03 | -0.72 | -2.87 |
Nuclear Periphery Foci | -1.06 | 19.11 | 20.88* | 6.60 | 1.31 | 7.33 | 24.96* | 25.12* | 19.79* | 1.97 | 2.04 | 31.28 | 33.72 | 24.19* | 2.01 |
Nucleolus | 11.91 | 7.86 | 11.04 | -6.29 | 0.49 | 3.41 | -0.14 | 3.48 | 2.35 | 2.90 | 13.21 | 4.76 | 7.40 | -3.77 | 0.96 |
Nucleus | -4.26 | -7.73 | -1.52 | 3.74 | 6.57 | -8.74 | -3.41 | -1.54 | 1.04 | 1.00 | -11.17 | -8.33 | -1.49 | 8.13 | 6.84 |
Peroxisomes | -1.18 | -2.17 | 0.37 | 1.30 | 2.60 | 3.99 | -1.12 | -0.34 | -2.02 | 0.62 | 1.82 | -1.52 | 2.63 | 0.46 | 3.28 |
Vacuole | -1.80 | -3.45 | -5.45 | -4.09 | -3.63 | -3.51 | -1.82 | -2.16 | -1.31 | -0.65 | -3.75 | -3.62 | -5.75 | -3.18 | -3.25 |
Vacuole Periphery | -2.04 | -0.87 | -4.26 | -3.55 | -4.18 | -4.51 | -1.16 | -1.70 | -0.03 | -1.53 | -4.89 | -0.88 | -4.34 | -2.00 | -4.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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