Standard name
Human Ortholog
Description Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0.05
Bud 0 0 0.06 0.05 0.06 0 0 0.06 0 0 0.06 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Cytoplasm 0.07 0.25 0.18 0.06 0.07 0.05 0 0 0 0 0 0 0.16 0.21 0.27 0.11 0.18 0.08 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Endosome 0 0.11 0 0 0.24 0 0 0 0 0 0 0 0.09 0.15 0.2 0.07 0.05 0.12 0.08 0.07 0 0.08 0.1 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0.43 0.35 0 0 0 0 0.05 0
Mitochondria 0.94 0.59 0.82 0.93 0.73 0.9 0.89 0.84 0.94 0.91 0.95 0.89 0.69 0.6 0.48 0.05 0 0.07 0.66 0.69 0.7 0.7 0.66 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.09 0.06 0.07 0.07 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.07 0.08 0.05 0.11 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.14 0 0 0 0 0 0 0 0.05 0.1 0.16 0.12 0 0.05 0 0 0 0 0 0.06
SpindlePole 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.11 0 0 0.05 0 0 0.05 0 0 0 0 0.11 0.09 0.07 0.12 0.14 0.17 0.08 0 0.07 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 3 0 0 1 5 0 2 5 0 2 8 20
Bud 1 6 3 6 12 9 10 26 12 17 13 9 8 16 4 3 4 1 0 1 0 0 4 9
Bud Neck 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 8
Bud Site 1 0 1 0 0 1 3 7 9 7 1 9 1 0 0 1 0 0
Cell Periphery 0 11 1 2 2 3 0 1 2 3 2 2 5 11 13 5 1 4 0 1 0 0 0 0
Cytoplasm 7 47 9 7 14 12 13 9 9 9 8 9 49 80 93 6 8 3 2 10 2 2 4 4
Endoplasmic Reticulum 5 2 1 1 1 3 3 1 0 1 1 1 11 8 4 2 3 2 1 3 2 1 2 3
Endosome 0 21 0 0 45 3 5 11 1 3 0 3 27 58 70 4 2 5 9 15 2 8 31 37
Golgi 0 1 0 0 1 0 1 0 0 0 0 0 2 4 5 23 19 14 3 4 1 3 15 16
Mitochondria 96 112 41 115 139 211 329 381 357 363 207 211 205 232 169 3 1 3 77 163 50 73 200 243
Nucleus 2 0 1 1 1 1 6 13 6 4 2 2 1 0 2 3 2 0 0 1 2 0 0 0
Nuclear Periphery 1 0 0 0 1 7 32 26 28 29 13 23 0 0 0 0 0 0 1 2 0 0 2 1
Nucleolus 0 0 1 2 0 6 25 37 20 42 15 22 1 1 4 2 1 1 0 0 0 0 1 0
Peroxisomes 0 5 0 6 26 3 1 11 2 2 0 3 14 40 56 7 0 2 1 4 3 2 10 26
SpindlePole 0 4 2 3 14 9 11 21 7 16 8 4 7 8 9 3 0 1 3 5 0 1 3 4
Vac/Vac Membrane 10 21 0 2 9 9 16 22 16 10 5 3 34 36 25 7 6 7 9 10 4 6 12 22
Unique Cell Count 102 189 50 124 191 234 368 451 380 397 219 236 297 384 350 57 44 40 118 236 72 105 302 406
Labelled Cell Count 123 231 61 145 265 278 455 566 470 506 275 301 368 494 454 70 52 43 118 236 72 105 302 406


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.0 4.6 4.8 5.4 4.7 4.6 5.1 4.3 4.8 4.6 4.6 5.0 5.5 5.4 8.5 8.2 8.4 5.4 5.7 6.0
Std Deviation (1e-4) 0.6 1.4 2.6 3.2 2.9 2.3 2.0 2.0 1.8 1.7 2.0 1.9 1.0 1.7 1.6 2.2 1.9 2.3 3.4 2.0 3.2
Intensity Change (Log2) 0.04 0.21 0.01 -0.01 0.14 -0.1 0.05 -0.02 -0.01 0.12 0.25 0.21 0.88 0.82 0.85 0.21 0.29 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0
Endosome 3.8 0 0 3.0 3.5 0 0 0
Golgi 0 0 0 0 0 5.1 5.2 4.6
Mitochondria -1.3 0.5 -1.9 -3.0 -4.5 -8.0 -7.8 -7.0
Nuclear Periphery 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0
Peroxisomes 2.8 0 0 0 3.0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0
Cytoplasm 0 0 -0.3 0.5 1.3 -1.1 0 -1.5
Nucleus 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9131 1.5407 1.3071 0.5021 1.0067 1.2239 1.3549 1.4205 1.3755 1.0181 1.2662 1.2559 2.7749 3.1992 2.7479 2.641 2.9336 3.1615
Actin 0.0177 0.0159 0.0155 0.001 0.0013 0.0656 0.0147 0.0156 0.0148 0.0044 0.0157 0.004 0.0253 0.0162 0.0151 0.0096 0.0019 0.0173
Bud 0.002 0.0048 0.0035 0.0005 0.0023 0.0004 0.0006 0.0054 0.0008 0.0004 0.0023 0.0003 0.0005 0.0014 0.0008 0.0016 0.0004 0.0003
Bud Neck 0.002 0.0015 0.0065 0.0001 0.0006 0.0035 0.0006 0.0011 0.0025 0.0001 0.0004 0.0006 0.0007 0.001 0.0018 0.0011 0.0002 0.0037
Bud Periphery 0.0052 0.0062 0.0077 0.0017 0.0246 0.0008 0.0009 0.0058 0.0016 0.0021 0.0065 0.0008 0.0014 0.0023 0.0022 0.0055 0.0004 0.0005
Bud Site 0.0068 0.0443 0.0309 0.0004 0.0008 0.0006 0.0018 0.0376 0.0122 0.0003 0.0013 0.0003 0.0021 0.0169 0.0093 0.0055 0.0008 0.0012
Cell Periphery 0.0016 0.0015 0.0015 0.0004 0.0005 0.0003 0.0003 0.0019 0.0007 0.0007 0.0004 0.0002 0.001 0.0009 0.0079 0.0018 0.0001 0.0002
Cytoplasm 0.0048 0.0141 0.0079 0.0006 0.0004 0.0005 0.0082 0.0239 0.0005 0.0001 0.0091 0.0005 0.0087 0.0097 0.0069 0.0134 0.0011 0.0051
Cytoplasmic Foci 0.046 0.04 0.0506 0.0054 0.0125 0.0127 0.0339 0.0426 0.0085 0.006 0.026 0.0201 0.0232 0.0322 0.022 0.0223 0.0853 0.0199
Eisosomes 0.0005 0.0004 0.0006 0.0003 0.0001 0.0006 0.0003 0.0006 0.002 0.0001 0.0004 0.0001 0.0006 0.0006 0.0023 0.0003 0.0001 0.0003
Endoplasmic Reticulum 0.0088 0.0037 0.0012 0.0004 0.0001 0.0005 0.0043 0.0068 0.0003 0.0001 0.0016 0.0003 0.0081 0.0016 0.0022 0.0005 0.0021 0.0014
Endosome 0.1045 0.0538 0.0641 0.0312 0.0227 0.0323 0.1165 0.0786 0.0385 0.0036 0.0609 0.043 0.0638 0.0855 0.0328 0.0358 0.0934 0.052
Golgi 0.0584 0.0666 0.0458 0.0469 0.0287 0.0436 0.0931 0.0616 0.0397 0.0111 0.0832 0.0614 0.0272 0.0445 0.0422 0.0297 0.1015 0.0654
Lipid Particles 0.0614 0.0158 0.0078 0.0007 0.0196 0.0233 0.0533 0.0077 0.0054 0.0007 0.0426 0.0037 0.0731 0.009 0.0209 0.0057 0.014 0.0043
Mitochondria 0.5456 0.6517 0.7034 0.902 0.8444 0.7962 0.6074 0.6329 0.8521 0.9323 0.6767 0.8418 0.6595 0.7139 0.7805 0.8084 0.63 0.7438
None 0.0046 0.0101 0.014 0.0002 0.0003 0.0003 0.0034 0.009 0.0002 0.0001 0.0031 0.0001 0.0223 0.0029 0.0108 0.0026 0.0003 0.0008
Nuclear Periphery 0.0081 0.0011 0.0003 0.0001 0 0.0007 0.0087 0.001 0.0001 0 0.0236 0.001 0.0132 0.0006 0.0005 0.0006 0.0017 0.0099
Nucleolus 0.0017 0.0005 0.0011 0 0.0002 0.0001 0.0011 0.0016 0.0002 0.0001 0.0006 0.0002 0.0013 0.0003 0.0003 0.0004 0.0001 0.0002
Nucleus 0.0023 0.0006 0.0007 0.0001 0.0002 0.0002 0.0031 0.0057 0.0001 0.0001 0.0019 0.0004 0.0021 0.0005 0.0003 0.0004 0.0003 0.0088
Peroxisomes 0.0729 0.0469 0.0276 0.0043 0.037 0.0126 0.0345 0.0474 0.0163 0.0334 0.0274 0.0173 0.0434 0.0419 0.0355 0.0276 0.0525 0.058
Punctate Nuclear 0.0034 0.0004 0.0003 0 0 0.0003 0.0034 0.0007 0.0001 0 0.0018 0.0003 0.008 0.0002 0.0002 0.0222 0.0002 0.0004
Vacuole 0.0268 0.0128 0.0063 0.0011 0.0018 0.0023 0.0047 0.0095 0.0017 0.0025 0.0075 0.0017 0.009 0.0119 0.0033 0.0032 0.0061 0.0022
Vacuole Periphery 0.0148 0.0073 0.0027 0.0024 0.0017 0.0027 0.0055 0.003 0.0019 0.0018 0.0073 0.002 0.0053 0.006 0.0023 0.0018 0.0074 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.1017 48.8051 36.2155 44.6606 26.0789 54.5653 33.7636 40.3175 38.8882 42.7747
Translational Efficiency 1.8996 1.2614 1.7861 1.9138 2.401 1.8503 1.974 1.639 1.9962 1.7476

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1812 1571 627 99 1391 1404 1628 1261 3203 2975 2255 1360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.06 740.24 958.72 1060.06 638.17 724.46 924.65 912.75 665.83 732.79 934.12 923.47
Standard Deviation 76.89 105.22 123.99 171.98 84.79 102.64 127.30 130.48 83.99 104.31 127.31 139.29
Intensity Change Log 2 0.107557 0.480673 0.625638 0.182965 0.534967 0.516279 0.144362 0.507074 0.574009

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000808 0.002183 0.006311 0.011888 0.001096 0.001363 0.009447 0.008806 0.000933 0.001796 0.008575 0.009030
Bud Neck 0.031799 0.013954 0.010183 0.003998 0.030541 0.026527 0.010444 0.004544 0.031253 0.019888 0.010371 0.004504
Bud Site 0.020308 0.037742 0.045535 0.049329 0.017172 0.031571 0.045114 0.037531 0.018946 0.034829 0.045231 0.038390
Cell Periphery 0.000253 0.000357 0.000320 0.001110 0.000693 0.000409 0.000451 0.000891 0.000444 0.000381 0.000414 0.000907
Cytoplasm 0.081698 0.026656 0.015089 0.005236 0.042227 0.028310 0.011042 0.008173 0.064557 0.027437 0.012167 0.007959
Cytoplasmic Foci 0.235066* 0.112505 0.123537 0.054642 0.138957 0.125465 0.059676 0.041839 0.193328* 0.118621 0.077432 0.042771
Eisosomes 0.000203 0.000145 0.000127 0.000130 0.000318 0.000165 0.000110 0.000104 0.000253 0.000154 0.000115 0.000106
Endoplasmic Reticulum 0.001124 0.000766 0.003167 0.001525 0.001309 0.000444 0.001401 0.000565 0.001204 0.000614 0.001892 0.000635
Endosome 0.073744 0.084706 0.121187 0.078289 0.068461 0.090477 0.093164 0.083035 0.071449 0.087429 0.100956 0.082690
Golgi 0.072164 0.147708* 0.114314* 0.091565 0.088983 0.118300* 0.078743 0.091373 0.079468 0.133830* 0.088633 0.091387
Lipid Particles 0.012636 0.003373 0.004889 0.003525 0.018411 0.007953 0.002587 0.000867 0.015144 0.005534 0.003227 0.001060
Mitochondria 0.342186* 0.442872* 0.261245* 0.429986* 0.479913* 0.439818* 0.468231* 0.589434* 0.401998* 0.441431* 0.410679* 0.577827*
Mitotic Spindle 0.011276 0.023357 0.107986* 0.083552 0.005091 0.013473 0.090456 0.032341 0.008590 0.018692 0.095330 0.036069
None 0.000907 0.000738 0.000323 0.000264 0.001753 0.000818 0.000299 0.000298 0.001275 0.000776 0.000306 0.000296
Nuclear Periphery 0.000570 0.000094 0.000807 0.000143 0.000604 0.000121 0.000437 0.000172 0.000585 0.000107 0.000540 0.000170
Nuclear Periphery Foci 0.000250 0.000203 0.001491 0.000346 0.000361 0.000164 0.000540 0.000210 0.000298 0.000184 0.000804 0.000220
Nucleolus 0.001526 0.000775 0.001489 0.000069 0.001510 0.001738 0.000993 0.000085 0.001519 0.001230 0.001131 0.000084
Nucleus 0.012221 0.002409 0.015591 0.000141 0.009684 0.003906 0.002976 0.000400 0.011119 0.003115 0.006483 0.000381
Peroxisomes 0.038011 0.055666 0.072661 0.151500* 0.057924 0.078219 0.069237 0.057330 0.046659 0.066309 0.070189 0.064185
Vacuole 0.046332 0.026506 0.068276 0.014524 0.019475 0.019357 0.036540 0.027343 0.034669 0.023132 0.045364 0.026410
Vacuole Periphery 0.016918 0.017284 0.025471 0.018238 0.015517 0.011404 0.018115 0.014659 0.016309 0.014509 0.020160 0.014919

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.08 -7.22 -3.31 -2.89 -1.62 -0.74 -6.89 -5.20 -5.06 0.37 -3.53 -8.58 -5.87 -5.24 -0.25
Bud Neck 7.88 9.91 13.78 6.27 4.49 1.12 6.74 9.32 9.35 4.76 5.58 11.49 16.13 11.01 5.88
Bud Site -6.36 -6.44 -2.37 -0.94 -0.28 -5.20 -9.47 -6.25 -1.71 2.04 -8.17 -11.47 -6.59 -1.18 2.00
Cell Periphery -2.54 -1.36 -2.04 -1.79 -1.87 3.10 2.38 -0.92 -2.41 -2.15 1.35 0.52 -2.44 -2.81 -2.59
Cytoplasm 13.46 15.68 20.44 8.89 4.22 3.73 9.70 10.83 8.17 2.05 13.03 19.91 22.19 11.09 3.40
Cytoplasmic Foci 19.99 15.47 20.91 7.40 7.72 2.25 16.01 19.93 18.17 5.65 16.86 27.61 36.86 22.50 11.09
Eisosomes 3.20 4.67 2.69 0.58 -0.00 2.54 3.49 3.60 4.98 0.84 3.50 5.02 5.34 4.97 1.41
Endoplasmic Reticulum 1.56 -5.42 -0.77 -1.44 2.94 4.17 -0.42 3.68 -1.27 3.36 3.82 -3.18 3.95 -0.24 6.06
Endosome -2.66 -7.33 -0.33 0.49 2.95 -4.55 -5.26 -2.93 1.42 1.97 -5.08 -8.13 -2.68 1.09 3.81
Golgi -13.68 -5.75 -1.22 3.42 1.21 -5.06 2.11 -0.42 4.43 -2.45 -13.64 -2.26 -2.53 8.12 -0.71
Lipid Particles 8.38 5.98 3.22 -0.04 0.60 5.44 9.53 10.83 6.60 3.70 9.06 12.19 15.00 7.22 4.91
Mitochondria -8.68 5.84 -2.17 0.32 -4.15 2.98 1.00 -7.76 -10.66 -8.84 -4.48 -0.66 -14.83 -11.36 -13.29
Mitotic Spindle -5.07 -12.28 -3.86 -3.19 1.25 -5.71 -19.42* -10.64 -6.71 11.56 -6.92 -22.28 -9.81 -5.86 12.66
None 1.01 4.24 4.89 3.83 0.81 1.58 2.61 2.61 3.00 0.03 1.82 3.84 3.86 4.45 0.24
Nuclear Periphery 1.97 -0.81 1.76 -1.18 3.42 2.12 0.66 1.86 -0.69 2.07 2.83 0.21 2.34 -1.05 3.31
Nuclear Periphery Foci 0.59 -3.98 -0.58 -0.87 3.41 2.16 -1.57 1.52 -1.09 3.60 1.95 -4.45 1.17 -0.81 5.24
Nucleolus 2.06 -0.13 4.91 4.27 4.08 -0.51 1.45 5.17 3.84 4.23 0.82 1.28 6.95 5.22 5.76
Nucleus 9.35 -1.42 12.64 5.84 4.82 3.99 4.47 7.11 5.27 3.87 9.14 2.92 13.53 7.05 5.95
Peroxisomes -4.58 -5.78 -4.35 -3.66 -2.96 -4.06 -2.22 0.16 3.72 2.10 -6.33 -6.36 -3.62 0.47 1.14
Vacuole 7.27 -4.94 7.54 3.05 9.62 0.05 -7.19 -3.12 -3.18 3.06 6.45 -5.13 2.92 -1.58 6.77
Vacuole Periphery -0.28 -3.38 -0.19 -0.13 0.94 2.99 -1.55 0.50 -2.19 1.92 1.78 -2.86 0.94 -0.29 3.04
Standard name
Human Ortholog
Description Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
Localization
Cell Percentages mitochondrion (85%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Trx3

Trx3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trx3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available