Standard name | |
---|---|
Human Ortholog | |
Description | Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 2 |
Bud | 0 | 0 | 0 | 1 | 2 | 3 | 9 | 8 | 7 | 10 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 11 | 8 | 2 | 10 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 5 |
Bud Site | 0 | 0 | 1 | 0 | 3 | 9 | 7 | 2 | 14 | 14 | 18 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 30 | 30 | 20 | 35 | 13 | 26 | 28 | 29 | 29 | 18 | 27 | 24 | 42 | 64 | 15 | 12 | 20 | 3 | 6 | 6 | 15 | 17 | 14 |
Endoplasmic Reticulum | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 4 | 3 | 1 | 1 | 1 | 0 | 2 | 4 |
Endosome | 0 | 6 | 0 | 1 | 5 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 5 | 0 | 2 | 5 | 2 | 3 | 8 | 12 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 6 | 1 | 0 | 1 | 1 |
Mitochondria | 92 | 6 | 11 | 19 | 33 | 86 | 226 | 134 | 296 | 186 | 252 | 170 | 1 | 1 | 0 | 13 | 13 | 12 | 7 | 3 | 13 | 27 | 33 | 34 |
Nucleus | 469 | 578 | 332 | 302 | 497 | 384 | 561 | 555 | 524 | 486 | 322 | 391 | 175 | 222 | 278 | 260 | 304 | 257 | 454 | 545 | 310 | 167 | 223 | 210 |
Nuclear Periphery | 1 | 1 | 0 | 2 | 3 | 1 | 3 | 0 | 15 | 1 | 4 | 3 | 0 | 0 | 0 | 7 | 1 | 1 | 0 | 2 | 1 | 1 | 3 | 3 |
Nucleolus | 1 | 0 | 0 | 0 | 5 | 1 | 30 | 15 | 18 | 25 | 10 | 3 | 0 | 0 | 0 | 2 | 4 | 0 | 1 | 3 | 1 | 8 | 14 | 16 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 3 | 3 | 3 | 4 | 3 | 9 | 3 | 10 | 3 | 3 | 3 | 2 | 7 | 0 | 4 | 1 | 8 | 11 | 5 | 8 | 16 | 18 |
Vac/Vac Membrane | 21 | 35 | 4 | 6 | 24 | 6 | 9 | 18 | 13 | 27 | 14 | 18 | 4 | 8 | 11 | 14 | 27 | 5 | 44 | 56 | 57 | 85 | 152 | 146 |
Unique Cell Count | 518 | 636 | 374 | 333 | 567 | 436 | 696 | 673 | 698 | 634 | 451 | 506 | 202 | 269 | 352 | 294 | 353 | 285 | 534 | 663 | 416 | 335 | 502 | 500 |
Labelled Cell Count | 595 | 660 | 383 | 354 | 611 | 507 | 874 | 772 | 921 | 789 | 649 | 638 | 209 | 278 | 364 | 326 | 376 | 301 | 534 | 663 | 416 | 335 | 502 | 500 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 6.9 | 5.8 | 5.6 | 6.0 | 5.2 | 4.9 | 5.6 | 4.5 | 5.0 | 4.5 | 4.9 | 6.0 | 6.2 | 6.1 | 7.9 | 8.5 | 8.3 | 5.8 | 6.0 | 6.3 |
Std Deviation (1e-4) | 0.7 | 1.4 | 1.2 | 1.5 | 1.6 | 1.4 | 1.2 | 1.3 | 1.1 | 1.3 | 1.3 | 1.3 | 1.1 | 1.3 | 1.2 | 1.7 | 1.9 | 1.9 | 1.6 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.05 | -0.17 | -0.25 | -0.05 | -0.38 | -0.23 | -0.38 | -0.24 | 0.05 | 0.1 | 0.07 | 0.44 | 0.55 | 0.52 | -0.01 | 0.04 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -1.1 | -3.2 | -3.0 | -2.6 | -2.6 | -2.3 | -2.5 | -1.6 | 1.5 | 3.0 | 4.1 | -1.5 | -2.7 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.8 | 2.0 | 7.3 | 0 | 7.6 | 0 | 10.2 | 0 | 11.1 | 0 | -2.4 | -3.2 | 1.0 | 0.6 | 0.9 |
Nucleus | 0.8 | -0.5 | -0.3 | -3.4 | -2.7 | -5.3 | -4.8 | -6.1 | -4.4 | -0.8 | -2.3 | -3.6 | -0.1 | -1.1 | 0.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.9 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.8 | 0 | 0 | 1.7 | 1.0 | 2.8 | 2.0 | 2.3 | 0 | 1.8 | 1.9 | 2.9 | 4.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8157 | 2.6661 | 2.7495 | 2.5638 | 1.6364 | 2.5605 | 1.5343 | 2.2582 | 2.4298 | 1.6963 | 1.267 | 1.8419 | 2.3618 | 2.1179 | 1.9282 | 2.1485 | 3.284 | 1.8049 |
Actin | 0.0365 | 0.0001 | 0.0324 | 0.0214 | 0.0002 | 0.0057 | 0.0082 | 0 | 0.0034 | 0 | 0.0005 | 0.0006 | 0.0127 | 0.0008 | 0.0001 | 0.033 | 0.0129 | 0.0025 |
Bud | 0.0006 | 0.0001 | 0.0014 | 0.0032 | 0 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0009 | 0 | 0 | 0.0004 | 0.0034 | 0.0001 |
Bud Neck | 0.0026 | 0.0004 | 0.0023 | 0.0005 | 0.0004 | 0.0016 | 0.0008 | 0.0002 | 0.0009 | 0.0001 | 0.0018 | 0.0016 | 0.007 | 0.0003 | 0.0001 | 0.0017 | 0.0018 | 0.0013 |
Bud Periphery | 0.0012 | 0.0002 | 0.0028 | 0.0058 | 0 | 0.0007 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0004 | 0.0057 | 0.0001 |
Bud Site | 0.003 | 0.0018 | 0.0026 | 0.002 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0012 | 0 | 0.0004 | 0.0002 | 0.0114 | 0.0004 | 0.0002 | 0.0018 | 0.0345 | 0.0002 |
Cell Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 |
Cytoplasm | 0.0227 | 0.0122 | 0.0027 | 0.0191 | 0.0068 | 0.0061 | 0.004 | 0.0036 | 0.0017 | 0.0003 | 0.0028 | 0.0013 | 0.0162 | 0.0075 | 0.0036 | 0.0047 | 0.0452 | 0.0022 |
Cytoplasmic Foci | 0.0129 | 0.0007 | 0.0064 | 0.0034 | 0.0001 | 0.0025 | 0.0007 | 0.0001 | 0.002 | 0 | 0.0002 | 0.0003 | 0.0138 | 0.0012 | 0.0059 | 0.0163 | 0.0242 | 0.004 |
Eisosomes | 0.0003 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0004 | 0.0006 | 0.0048 | 0.0002 | 0.0006 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0103 | 0.0009 | 0.0002 | 0.0004 | 0.0016 | 0.0001 |
Endosome | 0.03 | 0.0008 | 0.0118 | 0.023 | 0.0004 | 0.0051 | 0.0036 | 0.0009 | 0.0011 | 0 | 0.0006 | 0.0005 | 0.0306 | 0.0007 | 0.0014 | 0.0136 | 0.0377 | 0.0029 |
Golgi | 0.0101 | 0.0002 | 0.0045 | 0.0132 | 0 | 0.0055 | 0.0009 | 0 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.007 | 0.0002 | 0.0005 | 0.007 | 0.0141 | 0.003 |
Lipid Particles | 0.0134 | 0.0009 | 0.0032 | 0.018 | 0 | 0.0089 | 0.0034 | 0 | 0.0005 | 0 | 0.0001 | 0.0013 | 0.0225 | 0.0004 | 0.0013 | 0.0026 | 0.031 | 0.0114 |
Mitochondria | 0.0111 | 0.0016 | 0.0117 | 0.0393 | 0.0003 | 0.0035 | 0.0016 | 0.0001 | 0.0012 | 0.0001 | 0.0003 | 0.0022 | 0.022 | 0.0002 | 0.0055 | 0.0014 | 0.0175 | 0.0038 |
None | 0.0083 | 0.0197 | 0.0003 | 0.0033 | 0.0022 | 0.0066 | 0.0107 | 0.0031 | 0.0005 | 0.0001 | 0.0669 | 0.0002 | 0.0185 | 0.0064 | 0.0084 | 0.0005 | 0.0027 | 0.0045 |
Nuclear Periphery | 0.0255 | 0.0061 | 0.0078 | 0.0228 | 0.0109 | 0.0045 | 0.0145 | 0.0067 | 0.0041 | 0.0013 | 0.0051 | 0.0034 | 0.0207 | 0.0069 | 0.0033 | 0.001 | 0.0194 | 0.0031 |
Nucleolus | 0.0118 | 0.0102 | 0.0044 | 0.0046 | 0.0105 | 0.0119 | 0.0129 | 0.0066 | 0.0053 | 0.0031 | 0.0206 | 0.0139 | 0.014 | 0.007 | 0.0039 | 0.0039 | 0.0206 | 0.0126 |
Nucleus | 0.7798 | 0.932 | 0.891 | 0.801 | 0.9469 | 0.93 | 0.8967 | 0.9551 | 0.9655 | 0.9942 | 0.8834 | 0.9662 | 0.753 | 0.9581 | 0.9581 | 0.9012 | 0.6879 | 0.9297 |
Peroxisomes | 0.0034 | 0.0003 | 0.0035 | 0.0024 | 0 | 0.0037 | 0.0009 | 0 | 0.001 | 0 | 0 | 0.0031 | 0.0114 | 0.0018 | 0.0018 | 0.0058 | 0.0104 | 0.0051 |
Punctate Nuclear | 0.0089 | 0.0111 | 0.0007 | 0.0055 | 0.0208 | 0.001 | 0.0389 | 0.0219 | 0.0092 | 0.0008 | 0.0163 | 0.0043 | 0.008 | 0.006 | 0.0043 | 0.0027 | 0.0179 | 0.0131 |
Vacuole | 0.0102 | 0.0008 | 0.0071 | 0.003 | 0.0001 | 0.0008 | 0.0005 | 0.0009 | 0.0005 | 0 | 0.0003 | 0.0003 | 0.0116 | 0.0009 | 0.0007 | 0.0013 | 0.0075 | 0.0003 |
Vacuole Periphery | 0.004 | 0.0002 | 0.0022 | 0.0033 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0049 | 0.0002 | 0.0002 | 0.0002 | 0.0032 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.356 | 21.9195 | 15.5875 | 18.1282 | 27.1551 | 25.5276 | 15.9295 | 13.7118 | 19.683 | 31.0694 |
Translational Efficiency | 1.1162 | 0.8758 | 0.7812 | 0.8216 | 1.2499 | 2.0212 | 1.5095 | 1.1263 | 0.9861 | 1.3543 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1897 | 1354 | 2645 | 921 | 1467 | 1952 | 209 | 1496 | 3364 | 3306 | 2854 | 2417 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.93 | 812.08 | 895.60 | 943.66 | 691.07 | 764.42 | 967.39 | 950.92 | 710.16 | 783.94 | 900.86 | 948.15 |
Standard Deviation | 101.35 | 121.46 | 143.01 | 146.72 | 93.34 | 103.56 | 132.91 | 147.47 | 99.37 | 113.68 | 143.51 | 147.23 |
Intensity Change Log 2 | — | 0.163780 | 0.305013 | 0.380425 | — | 0.145534 | 0.485266 | 0.460492 | — | 0.154904 | 0.395799 | 0.420057 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000105 | 0.000469 | 0.000479 | 0.000872 | 0.000195 | 0.000154 | 0.000585 | 0.004295 | 0.000144 | 0.000283 | 0.000487 | 0.002991 |
Bud Neck | 0.003904 | 0.011769 | 0.019434 | 0.015768 | 0.003598 | 0.004267 | 0.003615 | 0.030796 | 0.003771 | 0.007339 | 0.018275 | 0.025070 |
Bud Site | 0.000958 | 0.002974 | 0.002593 | 0.004360 | 0.001184 | 0.000674 | 0.000601 | 0.021878 | 0.001056 | 0.001616 | 0.002447 | 0.015202 |
Cell Periphery | 0.000183 | 0.000219 | 0.000184 | 0.000181 | 0.000235 | 0.000131 | 0.000065 | 0.000236 | 0.000205 | 0.000167 | 0.000175 | 0.000215 |
Cytoplasm | 0.028695 | 0.026641 | 0.009763 | 0.025121 | 0.038495 | 0.008231 | 0.004770 | 0.063071 | 0.032969 | 0.015771 | 0.009397 | 0.048610 |
Cytoplasmic Foci | 0.002582 | 0.002512 | 0.000266 | 0.000318 | 0.004014 | 0.000344 | 0.001117 | 0.002112 | 0.003207 | 0.001232 | 0.000329 | 0.001428 |
Eisosomes | 0.000014 | 0.000021 | 0.000022 | 0.000029 | 0.000020 | 0.000013 | 0.000041 | 0.000031 | 0.000017 | 0.000016 | 0.000023 | 0.000030 |
Endoplasmic Reticulum | 0.003108 | 0.007944 | 0.004896 | 0.009114 | 0.002410 | 0.004017 | 0.005985 | 0.008274 | 0.002804 | 0.005625 | 0.004976 | 0.008594 |
Endosome | 0.000463 | 0.001437 | 0.000611 | 0.000695 | 0.000780 | 0.000825 | 0.000269 | 0.004964 | 0.000602 | 0.001076 | 0.000586 | 0.003337 |
Golgi | 0.000056 | 0.000436 | 0.000117 | 0.000124 | 0.000146 | 0.000036 | 0.000044 | 0.001940 | 0.000095 | 0.000200 | 0.000112 | 0.001248 |
Lipid Particles | 0.000653 | 0.001165 | 0.000079 | 0.000250 | 0.001026 | 0.000276 | 0.000595 | 0.000461 | 0.000815 | 0.000640 | 0.000117 | 0.000381 |
Mitochondria | 0.001126 | 0.001834 | 0.001920 | 0.002033 | 0.001142 | 0.000925 | 0.000644 | 0.008658 | 0.001133 | 0.001297 | 0.001827 | 0.006133 |
Mitotic Spindle | 0.001026 | 0.004294 | 0.003467 | 0.009268 | 0.002035 | 0.000614 | 0.000239 | 0.039775 | 0.001466 | 0.002121 | 0.003231 | 0.028150 |
None | 0.008831 | 0.007540 | 0.003090 | 0.006089 | 0.013489 | 0.004699 | 0.003631 | 0.007387 | 0.010862 | 0.005863 | 0.003129 | 0.006892 |
Nuclear Periphery | 0.001570 | 0.001547 | 0.001518 | 0.001546 | 0.000731 | 0.001025 | 0.000556 | 0.004402 | 0.001204 | 0.001239 | 0.001448 | 0.003314 |
Nuclear Periphery Foci | 0.000187 | 0.000677 | 0.000131 | 0.000249 | 0.000378 | 0.000231 | 0.000083 | 0.000812 | 0.000270 | 0.000414 | 0.000127 | 0.000597 |
Nucleolus | 0.003670 | 0.006810 | 0.001358 | 0.001358 | 0.007244 | 0.004606 | 0.003490 | 0.001557 | 0.005228 | 0.005509 | 0.001514 | 0.001481 |
Nucleus | 0.940617* | 0.915421* | 0.947260* | 0.913649* | 0.920097* | 0.966496* | 0.970330* | 0.768837* | 0.931669* | 0.945578* | 0.948949* | 0.824017* |
Peroxisomes | 0.000472 | 0.000656 | 0.000148 | 0.000168 | 0.000760 | 0.000146 | 0.000205 | 0.000769 | 0.000598 | 0.000355 | 0.000152 | 0.000540 |
Vacuole | 0.001491 | 0.004862 | 0.002444 | 0.008653 | 0.001742 | 0.001897 | 0.003061 | 0.025636 | 0.001601 | 0.003112 | 0.002489 | 0.019164 |
Vacuole Periphery | 0.000289 | 0.000770 | 0.000221 | 0.000158 | 0.000280 | 0.000392 | 0.000074 | 0.004112 | 0.000285 | 0.000547 | 0.000211 | 0.002605 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.01 | -13.28 | -14.72 | -8.28 | -8.80 | 1.16 | -6.78 | -2.89 | -2.96 | -1.87 | -4.94 | -12.13 | -3.85 | -3.55 | -2.98 |
Bud Neck | -7.25 | -16.60 | -12.38 | -5.07 | 0.71 | -1.04 | -2.59 | -15.65 | -15.67 | -13.98 | -6.15 | -17.42 | -19.40 | -15.76 | -4.79 |
Bud Site | -4.53 | -3.83 | -6.01 | -2.91 | -3.51 | 1.50 | 0.11 | -7.60 | -8.09 | -7.76 | -2.07 | -3.58 | -8.95 | -8.48 | -7.45 |
Cell Periphery | -1.43 | 0.77 | 0.50 | 2.01 | -0.34 | 1.99 | 3.01 | 1.05 | -2.82 | -6.61 | 1.49 | 1.66 | 1.11 | -0.76 | -1.06 |
Cytoplasm | 0.33 | 8.61 | 1.67 | 1.23 | -5.09 | 10.06 | 10.83 | -3.74 | -14.95 | -15.52 | 8.24 | 12.60 | -3.49 | -11.08 | -14.88 |
Cytoplasmic Foci | 0.09 | 4.54 | 4.39 | 4.10 | -0.88 | 6.03 | 4.11 | 4.54 | -3.42 | -0.41 | 4.29 | 7.31 | 5.81 | 1.65 | -3.46 |
Eisosomes | -4.30 | -7.55 | -14.11 | -9.34 | -10.15 | 5.22 | -6.92 | -8.94 | -14.36 | 2.72 | 0.66 | -7.54 | -16.19 | -16.74 | -10.49 |
Endoplasmic Reticulum | -11.41 | -8.93 | -15.60 | -5.53 | -11.45 | -5.37 | -8.29 | -17.17 | -11.94 | -1.43 | -11.17 | -13.27 | -22.55 | -12.37 | -13.45 |
Endosome | -3.44 | -0.32 | -2.80 | 2.34 | -1.39 | -0.12 | 3.11 | -3.59 | -3.31 | -4.40 | -2.54 | 0.43 | -4.13 | -2.39 | -3.96 |
Golgi | -1.47 | -0.90 | -3.19 | 1.16 | -0.55 | 2.84 | 2.05 | -2.56 | -3.03 | -2.91 | -0.94 | -0.07 | -2.85 | -1.89 | -2.74 |
Lipid Particles | -1.23 | 6.33 | 2.54 | 1.84 | -8.79 | 4.52 | 2.39 | 2.73 | -4.68 | -0.03 | 1.06 | 7.89 | 3.30 | 0.60 | -12.79 |
Mitochondria | -2.21 | -3.13 | -4.23 | -2.32 | -1.52 | 0.74 | 1.12 | -4.67 | -4.89 | -5.03 | -0.71 | -3.82 | -5.35 | -5.13 | -4.03 |
Mitotic Spindle | -2.74 | -3.17 | -4.38 | -2.70 | -3.21 | 1.72 | 1.15 | -8.26 | -8.95 | -8.71 | -1.04 | -2.45 | -9.50 | -9.09 | -8.38 |
None | 0.93 | 5.80 | 3.39 | 2.35 | -2.52 | 6.45 | 7.11 | 5.39 | -1.77 | -3.14 | 5.33 | 9.82 | 5.86 | 0.32 | -5.34 |
Nuclear Periphery | -0.06 | -0.81 | -4.94 | -5.40 | -4.03 | -1.98 | -1.56 | -6.97 | -6.23 | -6.56 | -0.46 | -2.88 | -7.33 | -7.08 | -5.81 |
Nuclear Periphery Foci | -3.49 | 0.80 | -4.03 | 1.69 | -4.58 | 1.15 | 1.60 | -2.04 | -4.73 | -5.79 | -1.50 | 1.79 | -3.99 | -2.19 | -7.14 |
Nucleolus | -3.63 | 5.74 | 3.51 | 5.68 | -1.29 | 3.40 | 4.78 | 9.64 | 6.52 | 2.63 | -0.74 | 10.37 | 9.78 | 8.55 | -0.38 |
Nucleus | 4.82 | -0.89 | 8.04 | 3.37 | 9.22 | -9.31 | -7.26 | 16.48 | 25.13 | 22.86 | -3.67 | -4.10 | 19.31 | 22.23 | 22.93 |
Peroxisomes | -0.90 | 1.64 | 1.48 | 3.33 | -0.62 | 2.51 | 2.17 | 1.34 | -2.22 | -1.48 | 1.38 | 2.78 | 1.67 | 0.56 | -1.88 |
Vacuole | -4.92 | -3.55 | -9.66 | -7.82 | -8.95 | -0.47 | -3.99 | -14.67 | -14.42 | -12.42 | -3.48 | -4.40 | -17.30 | -16.24 | -16.40 |
Vacuole Periphery | -2.45 | 0.69 | 1.10 | 3.67 | 0.58 | -0.83 | 4.24 | -3.06 | -2.77 | -3.45 | -2.19 | 1.05 | -2.86 | -1.97 | -3.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|