Standard name | |
---|---|
Human Ortholog | |
Description | Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 4 | 10 | 2 | 0 | 4 | 3 |
Bud | 1 | 5 | 5 | 5 | 4 | 1 | 11 | 6 | 6 | 8 | 6 | 6 | 2 | 0 | 1 | 9 | 4 | 3 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 3 | 0 | 0 | 0 | 3 | 1 | 3 | – | – | – | – | – | – |
Cell Periphery | 6 | 31 | 6 | 3 | 1 | 6 | 4 | 2 | 1 | 5 | 2 | 2 | 5 | 7 | 5 | 13 | 9 | 10 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 11 | 4 | 5 | 2 | 12 | 19 | 20 | 8 | 6 | 3 | 2 | 3 | 6 | 11 | 12 | 13 | 10 | 0 | 1 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 4 | 2 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 2 | 2 | 1 | 4 | 10 | 4 | 3 | 13 | 0 | 1 | 0 | 0 | 0 | 2 |
Endosome | 5 | 21 | 12 | 7 | 1 | 14 | 22 | 29 | 2 | 6 | 4 | 6 | 1 | 10 | 12 | 12 | 15 | 13 | 11 | 31 | 6 | 5 | 6 | 13 |
Golgi | 11 | 17 | 23 | 18 | 0 | 4 | 3 | 8 | 0 | 2 | 4 | 1 | 46 | 76 | 69 | 11 | 49 | 16 | 20 | 50 | 10 | 14 | 19 | 19 |
Mitochondria | 350 | 567 | 144 | 151 | 72 | 204 | 220 | 218 | 279 | 275 | 345 | 325 | 115 | 163 | 184 | 7 | 10 | 11 | 310 | 484 | 158 | 130 | 184 | 180 |
Nucleus | 2 | 3 | 1 | 0 | 2 | 5 | 8 | 6 | 1 | 5 | 6 | 5 | 0 | 0 | 1 | 0 | 4 | 7 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 16 | 14 | 4 | 3 | 5 | 19 | 27 | 25 | 34 | 22 | 24 | 24 | 10 | 2 | 10 | 5 | 5 | 10 | 4 | 5 | 1 | 0 | 3 | 3 |
Nucleolus | 10 | 14 | 3 | 3 | 7 | 20 | 25 | 22 | 26 | 18 | 25 | 21 | 1 | 2 | 2 | 4 | 0 | 5 | 1 | 2 | 0 | 1 | 1 | 1 |
Peroxisomes | 5 | 21 | 10 | 1 | 0 | 2 | 7 | 4 | 4 | 3 | 4 | 6 | 7 | 13 | 9 | 35 | 18 | 15 | 15 | 11 | 17 | 9 | 18 | 8 |
SpindlePole | 2 | 5 | 11 | 1 | 4 | 3 | 15 | 8 | 7 | 12 | 5 | 7 | 2 | 3 | 6 | 13 | 7 | 14 | 2 | 0 | 1 | 2 | 1 | 1 |
Vac/Vac Membrane | 51 | 136 | 13 | 8 | 5 | 27 | 31 | 29 | 12 | 19 | 18 | 12 | 19 | 17 | 39 | 12 | 16 | 28 | 9 | 37 | 3 | 5 | 10 | 9 |
Unique Cell Count | 375 | 633 | 179 | 168 | 87 | 257 | 302 | 297 | 302 | 312 | 373 | 348 | 144 | 222 | 261 | 113 | 127 | 132 | 382 | 646 | 206 | 173 | 255 | 251 |
Labelled Cell Count | 464 | 857 | 240 | 208 | 105 | 319 | 395 | 378 | 384 | 388 | 451 | 422 | 212 | 306 | 360 | 140 | 155 | 160 | 382 | 646 | 206 | 173 | 255 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 7.7 | 7.9 | 7.7 | 5.6 | 7.6 | 7.3 | 7.7 | 6.0 | 6.7 | 6.4 | 6.1 | 8.6 | 8.4 | 7.8 | 6.6 | 8.0 | 7.6 | 9.8 | 10.0 | 10.0 |
Std Deviation (1e-4) | 1.6 | 1.3 | 1.3 | 1.3 | 1.2 | 1.7 | 1.7 | 1.6 | 1.3 | 1.4 | 1.5 | 1.3 | 1.5 | 1.5 | 1.4 | 1.7 | 2.2 | 2.0 | 2.3 | 2.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.49 | -0.06 | -0.12 | -0.04 | -0.4 | -0.24 | -0.3 | -0.37 | 0.12 | 0.09 | -0.02 | -0.26 | 0.02 | -0.04 | 0.31 | 0.34 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0 | 2.8 | 1.5 | 1.7 |
Cytoplasm | 0 | 0 | 1.3 | 2.0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 3.1 | 3.0 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 |
Endosome | -1.0 | 0 | -0.5 | 0.2 | 1.2 | -3.8 | -2.7 | -3.7 | -3.0 | -2.7 | -1.0 | -1.0 | 1.2 | 1.6 | 1.0 |
Golgi | -0.6 | -3.5 | -4.8 | -5.6 | -4.3 | -6.4 | -5.9 | -6.0 | -6.6 | 4.2 | 4.9 | 3.4 | -0.8 | 5.2 | -0.2 |
Mitochondria | 2.5 | 0.5 | -0.3 | -1.9 | -1.7 | 3.9 | 2.3 | 4.2 | 4.5 | -0.1 | -1.7 | -2.4 | -12.4 | -12.5 | -12.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.4 | 2.9 | 2.7 | 3.5 | 2.3 | 2.1 | 2.3 | 2.1 | 0 | 0.9 | 0 | 0 | 2.2 |
Nucleolus | 0 | 0 | 2.8 | 3.0 | 2.7 | 3.1 | 2.2 | 2.5 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.7 | 0 | 0 | -1.9 | -2.7 | -2.7 | 0 | 0 | -2.4 | -0.3 | 0.1 | -1.1 | 5.9 | 2.6 | 1.9 |
SpindlePole | -2.8 | 0 | -2.9 | -0.6 | -1.9 | -2.1 | -1.2 | -3.1 | -2.5 | -2.2 | -2.6 | -2.1 | 1.6 | -0.2 | 1.4 |
Vacuole | -1.0 | -0.5 | 1.2 | 1.1 | 0.9 | -1.6 | -0.5 | -1.2 | -2.0 | 1.8 | 0.1 | 2.5 | 1.0 | 1.6 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6081 | 4.2481 | 4.2884 | 3.9325 | 3.7558 | 4.6545 | 9.5179 | 9.9732 | 9.6153 | 9.147 | 9.1517 | 10.3324 | 7.312 | 7.1276 | 7.1251 | 6.6852 | 6.5865 | 7.3326 |
Actin | 0.0237 | 0.0039 | 0.0093 | 0.0278 | 0.0069 | 0.0022 | 0.0177 | 0.0076 | 0.0038 | 0.0017 | 0.056 | 0.0127 | 0.0214 | 0.0189 | 0.0123 | 0.0158 | 0.0007 | 0.0021 |
Bud | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0011 | 0.0002 | 0.0009 | 0.0012 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0002 | 0.0008 | 0 | 0.0001 |
Bud Periphery | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0032 | 0.0001 | 0.0006 | 0.0009 | 0.0006 | 0.0006 | 0.0003 | 0.0003 | 0.0002 |
Bud Site | 0.0008 | 0.0008 | 0.0016 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0.0008 | 0.0003 | 0.0008 | 0.0009 | 0.0026 | 0.0006 | 0.0016 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0037 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0014 | 0.0001 | 0.0015 | 0 | 0.0002 | 0.0002 |
Cytoplasmic Foci | 0.0062 | 0.0069 | 0.0029 | 0.0037 | 0.0039 | 0.0029 | 0.0268 | 0.0067 | 0.0012 | 0.0131 | 0.0043 | 0.001 | 0.008 | 0.0056 | 0.0099 | 0.0008 | 0.002 | 0.0009 |
Eisosomes | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0003 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0011 | 0 | 0.0003 | 0.0007 | 0 | 0.0002 | 0.0023 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0008 |
Endosome | 0.0206 | 0.0172 | 0.0045 | 0.0031 | 0.0216 | 0.0135 | 0.0147 | 0.0113 | 0.0144 | 0.0566 | 0.0044 | 0.0038 | 0.0188 | 0.0155 | 0.0165 | 0.0007 | 0.0054 | 0.0079 |
Golgi | 0.0298 | 0.0462 | 0.0105 | 0.0089 | 0.0353 | 0.0293 | 0.0102 | 0.0167 | 0.0099 | 0.0123 | 0.0113 | 0.0109 | 0.0195 | 0.0502 | 0.0212 | 0.0016 | 0.0086 | 0.023 |
Lipid Particles | 0.0177 | 0.0157 | 0.0104 | 0.0033 | 0.0052 | 0.0054 | 0.0157 | 0.0081 | 0.0121 | 0.0502 | 0.0113 | 0.0037 | 0.0114 | 0.0104 | 0.0043 | 0.0033 | 0.0311 | 0.0024 |
Mitochondria | 0.8725 | 0.8939 | 0.9266 | 0.9251 | 0.9197 | 0.9333 | 0.8848 | 0.931 | 0.9473 | 0.8247 | 0.8595 | 0.9463 | 0.8802 | 0.8696 | 0.9049 | 0.9667 | 0.9012 | 0.954 |
None | 0.0013 | 0 | 0.0044 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0019 | 0 | 0.0014 | 0.002 | 0 | 0.0007 | 0.0016 | 0.0001 | 0.0019 | 0 | 0.0132 | 0.0013 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0036 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0 |
Nucleus | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0009 | 0.0004 | 0.0001 | 0.0009 | 0 | 0.0161 | 0.0002 |
Peroxisomes | 0.0152 | 0.0105 | 0.0225 | 0.0257 | 0.0029 | 0.0114 | 0.0171 | 0.0146 | 0.006 | 0.0263 | 0.051 | 0.0061 | 0.0165 | 0.0199 | 0.0146 | 0.0069 | 0.0133 | 0.0035 |
Punctate Nuclear | 0.0004 | 0 | 0.0006 | 0 | 0 | 0 | 0.0029 | 0 | 0.0001 | 0.0012 | 0 | 0.002 | 0.0064 | 0.0001 | 0.0019 | 0.0001 | 0.0002 | 0.0001 |
Vacuole | 0.0021 | 0.0013 | 0.0012 | 0.0001 | 0.001 | 0.0004 | 0.0008 | 0.0009 | 0.0009 | 0.0022 | 0.0001 | 0.0016 | 0.0048 | 0.0025 | 0.0012 | 0.0001 | 0.0019 | 0.0007 |
Vacuole Periphery | 0.0041 | 0.0016 | 0.0031 | 0.0003 | 0.0013 | 0.0009 | 0.0016 | 0.0007 | 0.0017 | 0.0022 | 0.0002 | 0.003 | 0.0029 | 0.0023 | 0.0019 | 0.0002 | 0.0047 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.6944 | 48.9965 | 49.7524 | 28.0548 | 41.1418 | 46.1299 | 32.9182 | 21.1177 | 25.4913 | 37.5747 |
Translational Efficiency | 2.3101 | 2.6646 | 1.5724 | 2.2072 | 2.112 | 1.6478 | 1.8013 | 2.4491 | 1.4079 | 1.8123 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
389 | 557 | 1611 | 2188 | 1206 | 2022 | 1061 | 1904 | 1595 | 2579 | 2672 | 4092 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 726.67 | 991.51 | 1108.87 | 1097.92 | 876.62 | 1033.94 | 1080.52 | 1136.30 | 840.05 | 1024.78 | 1097.61 | 1115.78 |
Standard Deviation | 127.27 | 147.15 | 187.10 | 169.48 | 139.65 | 144.37 | 159.73 | 190.27 | 151.14 | 146.02 | 177.28 | 180.47 |
Intensity Change Log 2 | — | 0.448327 | 0.609718 | 0.595401 | — | 0.238129 | 0.301702 | 0.374320 | — | 0.337207 | 0.449494 | 0.478736 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002685 | 0.003513 | 0.004380 | 0.001966 | 0.001472 | 0.001223 | 0.003130 | 0.004458 | 0.001768 | 0.001718 | 0.003884 | 0.003125 |
Bud Neck | 0.005185 | 0.002539 | 0.001652 | 0.001648 | 0.001965 | 0.001324 | 0.003278 | 0.001305 | 0.002750 | 0.001586 | 0.002297 | 0.001488 |
Bud Site | 0.004870 | 0.003140 | 0.003263 | 0.011267 | 0.003477 | 0.003508 | 0.009011 | 0.008431 | 0.003817 | 0.003429 | 0.005546 | 0.009947 |
Cell Periphery | 0.001996 | 0.000158 | 0.000155 | 0.000566 | 0.000398 | 0.000219 | 0.000265 | 0.000643 | 0.000788 | 0.000206 | 0.000199 | 0.000602 |
Cytoplasm | 0.000427 | 0.000571 | 0.000710 | 0.001826 | 0.000620 | 0.000473 | 0.001028 | 0.001547 | 0.000573 | 0.000494 | 0.000836 | 0.001696 |
Cytoplasmic Foci | 0.010286 | 0.029959 | 0.032034 | 0.013734 | 0.026530 | 0.019908 | 0.035131 | 0.009624 | 0.022568 | 0.022079 | 0.033264 | 0.011821 |
Eisosomes | 0.000067 | 0.000102 | 0.000079 | 0.000058 | 0.000056 | 0.000051 | 0.000071 | 0.000144 | 0.000059 | 0.000062 | 0.000076 | 0.000098 |
Endoplasmic Reticulum | 0.000358 | 0.000185 | 0.000392 | 0.000989 | 0.000322 | 0.000138 | 0.000930 | 0.000388 | 0.000331 | 0.000148 | 0.000605 | 0.000709 |
Endosome | 0.020187 | 0.034529 | 0.024565 | 0.030713 | 0.025600 | 0.033643 | 0.033597 | 0.015153 | 0.024280 | 0.033835 | 0.028152 | 0.023473 |
Golgi | 0.009301 | 0.048130 | 0.056095 | 0.062769 | 0.029392 | 0.027520 | 0.049358 | 0.039379 | 0.024492 | 0.031971 | 0.053420 | 0.051886 |
Lipid Particles | 0.008094 | 0.005700 | 0.005558 | 0.003754 | 0.011281 | 0.005749 | 0.003549 | 0.002136 | 0.010504 | 0.005738 | 0.004760 | 0.003002 |
Mitochondria | 0.897358* | 0.824016* | 0.813385* | 0.792195* | 0.860443* | 0.877766* | 0.780907* | 0.879010* | 0.869446* | 0.866157* | 0.800489* | 0.832590* |
Mitotic Spindle | 0.005498 | 0.002771 | 0.012398 | 0.010183 | 0.003146 | 0.001345 | 0.026027 | 0.004740 | 0.003720 | 0.001653 | 0.017810 | 0.007650 |
None | 0.000366 | 0.000224 | 0.000534 | 0.000574 | 0.000285 | 0.000206 | 0.000343 | 0.000578 | 0.000305 | 0.000210 | 0.000458 | 0.000576 |
Nuclear Periphery | 0.000048 | 0.000081 | 0.001268 | 0.001157 | 0.000240 | 0.000119 | 0.001425 | 0.000465 | 0.000193 | 0.000111 | 0.001330 | 0.000835 |
Nuclear Periphery Foci | 0.000176 | 0.000207 | 0.000876 | 0.000842 | 0.000396 | 0.000181 | 0.001115 | 0.000451 | 0.000342 | 0.000187 | 0.000971 | 0.000661 |
Nucleolus | 0.000292 | 0.000183 | 0.000273 | 0.000177 | 0.000607 | 0.000118 | 0.000448 | 0.000075 | 0.000530 | 0.000132 | 0.000343 | 0.000130 |
Nucleus | 0.000146 | 0.000091 | 0.000107 | 0.000189 | 0.000447 | 0.000126 | 0.000294 | 0.000062 | 0.000373 | 0.000119 | 0.000181 | 0.000130 |
Peroxisomes | 0.027685 | 0.038756 | 0.010686 | 0.003823 | 0.024981 | 0.015960 | 0.022042 | 0.005318 | 0.025640 | 0.020883 | 0.015195 | 0.004518 |
Vacuole | 0.001888 | 0.002626 | 0.013073 | 0.026338 | 0.003381 | 0.005577 | 0.014486 | 0.010804 | 0.003017 | 0.004940 | 0.013634 | 0.019110 |
Vacuole Periphery | 0.003087 | 0.002519 | 0.018517 | 0.035232 | 0.004962 | 0.004845 | 0.013566 | 0.015289 | 0.004504 | 0.004343 | 0.016551 | 0.025953 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.45 | -0.92 | 0.41 | 1.96 | 3.08 | 0.70 | -3.67 | -3.50 | -3.75 | -1.46 | 0.10 | -3.30 | -2.22 | -2.86 | 1.22 |
Bud Neck | 1.06 | 1.53 | 1.53 | 0.90 | 0.00 | 1.16 | -2.00 | 1.17 | -0.01 | 4.05 | 1.59 | 0.62 | 1.80 | 0.27 | 3.07 |
Bud Site | 0.87 | 0.89 | -3.05 | -5.02 | -5.86 | -0.02 | -2.91 | -3.17 | -3.48 | 0.29 | 0.36 | -1.56 | -4.98 | -6.03 | -3.86 |
Cell Periphery | 2.56 | 2.56 | 1.98 | -5.17 | -5.25 | 0.87 | 0.64 | -0.96 | -2.70 | -2.37 | 2.47 | 2.51 | 0.75 | -4.57 | -4.76 |
Cytoplasm | -1.05 | -1.69 | -3.69 | -3.43 | -2.96 | 0.88 | -1.94 | -2.63 | -3.23 | -1.45 | 0.60 | -1.80 | -4.19 | -4.76 | -3.30 |
Cytoplasmic Foci | -4.57 | -6.26 | -1.12 | 4.86 | 8.42 | 2.71 | -2.62 | 7.62 | 6.39 | 9.36 | 0.25 | -4.62 | 5.93 | 7.21 | 12.66 |
Eisosomes | -2.77 | -1.27 | 1.08 | 4.46 | 4.52 | 1.06 | -2.66 | -1.54 | -1.62 | -1.27 | -0.78 | -3.78 | -1.46 | -1.33 | -0.83 |
Endoplasmic Reticulum | 1.76 | -0.32 | -4.10 | -6.35 | -4.52 | 4.09 | -1.33 | -1.01 | -4.82 | 1.18 | 4.40 | -1.46 | -4.72 | -7.92 | -0.53 |
Endosome | -2.76 | -1.10 | -2.61 | 0.93 | -2.67 | -2.79 | -2.68 | 4.29 | 7.90 | 7.46 | -3.79 | -1.75 | 0.36 | 5.04 | 2.78 |
Golgi | -7.78 | -14.05 | -14.98 | -2.51 | -1.49 | 0.68 | -4.70 | -2.93 | -3.85 | 2.23 | -3.11 | -9.98 | -9.95 | -7.32 | 0.48 |
Lipid Particles | 0.79 | 0.94 | 1.59 | 1.18 | 2.03 | 2.91 | 4.17 | 5.17 | 4.13 | 1.87 | 2.95 | 3.77 | 4.96 | 3.39 | 2.97 |
Mitochondria | 4.20 | 6.02 | 7.66 | 2.27 | 2.23 | -1.89 | 6.50 | -1.93 | -0.09 | -8.75 | 0.40 | 8.05 | 4.78 | 5.12 | -4.43 |
Mitotic Spindle | 1.40 | -2.83 | -2.17 | -6.03 | 1.14 | 3.56 | -7.75 | -1.96 | -4.84 | 7.09 | 3.40 | -8.76 | -4.45 | -8.65 | 6.17 |
None | 2.38 | -2.74 | -3.29 | -10.79 | -1.17 | 3.53 | -2.37 | -6.36 | -8.59 | -5.28 | 4.25 | -6.16 | -8.61 | -14.05 | -4.19 |
Nuclear Periphery | -2.14 | -6.33 | -6.92 | -6.70 | 0.44 | 2.05 | -2.56 | -2.37 | -4.37 | 2.06 | 1.83 | -5.16 | -6.28 | -7.70 | 2.10 |
Nuclear Periphery Foci | -0.31 | -5.37 | -5.59 | -4.65 | 0.20 | 2.60 | -2.20 | -0.42 | -2.03 | 1.91 | 2.33 | -4.01 | -3.18 | -5.26 | 1.83 |
Nucleolus | 0.66 | 0.14 | 0.90 | 0.07 | 0.91 | 1.93 | 0.47 | 2.10 | 1.32 | 2.11 | 2.04 | 0.85 | 2.06 | 0.02 | 2.27 |
Nucleus | 0.87 | 0.77 | -0.71 | -1.73 | -1.83 | 1.44 | 0.58 | 1.76 | 0.96 | 2.42 | 1.50 | 1.11 | 1.46 | -0.29 | 1.13 |
Peroxisomes | -1.49 | 3.05 | 4.38 | 6.89 | 4.92 | 2.94 | 0.78 | 7.04 | 6.59 | 6.31 | 1.64 | 3.79 | 8.54 | 9.97 | 7.88 |
Vacuole | -1.29 | -9.56 | -16.17 | -15.82 | -7.32 | -2.99 | -7.58 | -6.70 | -4.47 | 2.03 | -3.28 | -11.48 | -16.43 | -13.72 | -4.39 |
Vacuole Periphery | 0.73 | -10.66 | -16.37 | -17.76 | -7.56 | 0.16 | -7.33 | -7.23 | -7.46 | -1.04 | 0.28 | -12.43 | -17.22 | -17.72 | -6.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|