Standard name | |
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Human Ortholog | |
Description | Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 4 | 5 | 6 | 18 | 30 | 29 | 16 | 33 | 26 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 5 | 2 | 2 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 51 | 191 | 153 | 233 | 215 | 390 | 465 | 541 | 463 | 451 | 447 | 559 | 13 | 24 | 30 | 60 | 58 | 69 | 5 | 18 | 9 | 3 | 7 | 10 |
Endoplasmic Reticulum | 6 | 10 | 5 | 1 | 8 | 11 | 19 | 20 | 4 | 4 | 7 | 6 | 4 | 9 | 9 | 5 | 3 | 4 | 0 | 3 | 8 | 1 | 3 | 1 |
Endosome | 10 | 30 | 86 | 95 | 75 | 37 | 36 | 52 | 15 | 25 | 20 | 26 | 3 | 11 | 7 | 11 | 6 | 17 | 14 | 37 | 63 | 16 | 29 | 42 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Mitochondria | 14 | 50 | 90 | 126 | 113 | 346 | 556 | 357 | 336 | 308 | 366 | 373 | 5 | 0 | 0 | 7 | 8 | 2 | 7 | 20 | 20 | 4 | 11 | 8 |
Nucleus | 23 | 14 | 4 | 1 | 8 | 10 | 19 | 16 | 6 | 3 | 4 | 4 | 7 | 10 | 15 | 11 | 12 | 15 | 5 | 5 | 1 | 3 | 5 | 3 |
Nuclear Periphery | 1 | 6 | 3 | 0 | 1 | 1 | 21 | 13 | 0 | 5 | 1 | 5 | 1 | 4 | 4 | 7 | 0 | 3 | 4 | 6 | 43 | 3 | 5 | 8 |
Nucleolus | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 4 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 |
Vac/Vac Membrane | 231 | 289 | 401 | 234 | 325 | 323 | 269 | 310 | 190 | 211 | 197 | 289 | 51 | 156 | 164 | 177 | 167 | 182 | 266 | 442 | 504 | 118 | 223 | 247 |
Unique Cell Count | 284 | 493 | 605 | 514 | 582 | 874 | 1075 | 1067 | 780 | 780 | 817 | 992 | 75 | 187 | 212 | 240 | 223 | 250 | 308 | 549 | 665 | 153 | 298 | 331 |
Labelled Cell Count | 336 | 593 | 744 | 696 | 752 | 1124 | 1408 | 1349 | 1052 | 1030 | 1080 | 1293 | 86 | 214 | 233 | 283 | 255 | 293 | 308 | 549 | 665 | 153 | 298 | 331 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 4.9 | 5.0 | 5.0 | 5.2 | 4.6 | 4.3 | 4.8 | 4.6 | 4.6 | 4.6 | 4.4 | 6.0 | 6.2 | 6.5 | 7.0 | 6.7 | 6.7 | 5.7 | 5.9 | 6.2 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.2 | 1.4 | 1.0 | 1.5 | 0.9 | 1.1 | 1.0 | 0.9 | 0.9 | 0.8 | 1.1 | 0.8 | 0.8 | 1.3 | 1.3 | 1.4 | 0.8 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.06 | -0.11 | -0.2 | -0.05 | -0.12 | -0.12 | -0.12 | -0.17 | 0.27 | 0.32 | 0.39 | 0.49 | 0.43 | 0.43 | 0.2 | 0.25 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7.0 | 4.3 | 7.6 | 7.3 | 10.1 | 12.7 | 12.1 | 11.1 | 12.1 | -1.5 | -3.6 | -3.3 | -0.1 | 0.2 | 0.7 |
Endoplasmic Reticulum | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.9 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -3.0 | -4.3 | -4.0 | -4.7 | -3.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.1 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | -5.6 | -6.0 | -4.9 | -4.5 | -6.0 |
Nucleus | 0 | 1.2 | 0.9 | 1.9 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -7.0 | -3.7 | -11.1 | -16.6 | -14.8 | -15.6 | -14.6 | -15.9 | -14.5 | 0.3 | 4.5 | 3.0 | 2.1 | 2.4 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5486 | 0.9932 | 0.9532 | 0.9094 | 0.2312 | 0.9505 | 2.476 | 3.1245 | 2.8752 | 2.9287 | 2.6411 | 2.9634 | 0.9174 | 1.8203 | 1.3578 | 0.7687 | 1.1823 | 1.3626 |
Actin | 0.0003 | 0.0011 | 0 | 0.0001 | 0 | 0.0001 | 0.0025 | 0 | 0.0011 | 0 | 0 | 0 | 0.0937 | 0.0002 | 0.0002 | 0 | 0 | 0 |
Bud | 0.0003 | 0.0011 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.001 | 0.0016 | 0.0012 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0005 | 0.0003 |
Bud Neck | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0.0009 | 0.0005 | 0.0077 | 0.0004 | 0.0007 | 0.0004 | 0.0071 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0005 | 0.0012 | 0.0013 | 0.0007 | 0.0005 | 0.0008 | 0.0009 | 0.0024 | 0.0017 | 0.0019 | 0.0005 | 0.0009 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0009 | 0.0007 |
Bud Site | 0.0026 | 0.0016 | 0.0004 | 0.0005 | 0.0007 | 0.0003 | 0.0015 | 0.0023 | 0.0039 | 0.0009 | 0.0003 | 0.0003 | 0.0009 | 0.0083 | 0.0007 | 0.0001 | 0.0004 | 0.0001 |
Cell Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0014 | 0.0004 | 0.0002 | 0.0004 | 0.0002 |
Cytoplasm | 0.02 | 0.0558 | 0.0498 | 0.044 | 0.0122 | 0.046 | 0.0289 | 0.0482 | 0.0393 | 0.021 | 0.0137 | 0.037 | 0.016 | 0.054 | 0.0984 | 0.0012 | 0.055 | 0.0219 |
Cytoplasmic Foci | 0.0177 | 0.0203 | 0.0185 | 0.0312 | 0.0241 | 0.0325 | 0.0165 | 0.0143 | 0.0201 | 0.016 | 0.0087 | 0.0193 | 0.0255 | 0.017 | 0.0307 | 0.0007 | 0.0082 | 0.0092 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0065 | 0.0078 | 0.0031 | 0.0036 | 0.0024 | 0.0058 | 0.0061 | 0.0141 | 0.0083 | 0.0023 | 0.0027 | 0.0049 | 0.0025 | 0.0093 | 0.0032 | 0.0007 | 0.0084 | 0.0019 |
Endosome | 0.3036 | 0.2641 | 0.2956 | 0.2853 | 0.2756 | 0.3144 | 0.2785 | 0.2087 | 0.2868 | 0.3173 | 0.227 | 0.257 | 0.2213 | 0.2214 | 0.2174 | 0.1373 | 0.1832 | 0.2238 |
Golgi | 0.0048 | 0.0035 | 0.0047 | 0.006 | 0.0038 | 0.0041 | 0.0073 | 0.0028 | 0.0067 | 0.0045 | 0.002 | 0.0034 | 0.005 | 0.0093 | 0.0209 | 0.0011 | 0.0013 | 0.0017 |
Lipid Particles | 0.0098 | 0.009 | 0.0044 | 0.0072 | 0.0048 | 0.0042 | 0.0064 | 0.0047 | 0.005 | 0.0007 | 0.0035 | 0.0048 | 0.0489 | 0.0157 | 0.0012 | 0.0009 | 0.0026 | 0.0006 |
Mitochondria | 0.0077 | 0.0109 | 0.0133 | 0.0137 | 0.0039 | 0.0103 | 0.0079 | 0.0083 | 0.0232 | 0.002 | 0.0029 | 0.0108 | 0.0048 | 0.0166 | 0.0008 | 0.0007 | 0.0045 | 0.0015 |
None | 0.0297 | 0.0208 | 0.0276 | 0.0913 | 0.0431 | 0.0304 | 0.0352 | 0.0226 | 0.0285 | 0.0024 | 0.0653 | 0.0184 | 0.077 | 0.0692 | 0.0244 | 0.0001 | 0.1478 | 0.0036 |
Nuclear Periphery | 0.0332 | 0.036 | 0.0128 | 0.0098 | 0.0089 | 0.0205 | 0.0151 | 0.0258 | 0.019 | 0.0059 | 0.0081 | 0.0197 | 0.0042 | 0.0215 | 0.0071 | 0.0052 | 0.017 | 0.0116 |
Nucleolus | 0.0017 | 0.0009 | 0.0016 | 0.0009 | 0.0014 | 0.0012 | 0.0025 | 0.0015 | 0.0028 | 0.0008 | 0.0005 | 0.0011 | 0.0006 | 0.0052 | 0.0014 | 0.0003 | 0.0021 | 0.0005 |
Nucleus | 0.0087 | 0.0048 | 0.0046 | 0.0033 | 0.0034 | 0.0063 | 0.0077 | 0.0047 | 0.0071 | 0.0051 | 0.0015 | 0.0062 | 0.0019 | 0.0048 | 0.0328 | 0.0007 | 0.0135 | 0.0039 |
Peroxisomes | 0.0012 | 0.0024 | 0.0007 | 0.0014 | 0.0006 | 0.0013 | 0.0065 | 0.0005 | 0.0012 | 0.0005 | 0.0003 | 0.0008 | 0.01 | 0.001 | 0.0017 | 0.0001 | 0.0003 | 0.0002 |
Punctate Nuclear | 0.0022 | 0.0009 | 0.0006 | 0.0011 | 0.0013 | 0.0021 | 0.0026 | 0.0006 | 0.0012 | 0.0003 | 0.0002 | 0.0012 | 0.0015 | 0.0014 | 0.0002 | 0 | 0.001 | 0.0001 |
Vacuole | 0.3821 | 0.3146 | 0.3492 | 0.2859 | 0.3305 | 0.3038 | 0.4028 | 0.3829 | 0.3368 | 0.4366 | 0.3818 | 0.3437 | 0.3812 | 0.4169 | 0.4265 | 0.4929 | 0.2434 | 0.4078 |
Vacuole Periphery | 0.1663 | 0.2425 | 0.2108 | 0.2127 | 0.2819 | 0.2148 | 0.1695 | 0.2531 | 0.2045 | 0.1729 | 0.2801 | 0.2693 | 0.1036 | 0.1183 | 0.1312 | 0.3575 | 0.3094 | 0.3104 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.7332 | 12.3187 | 15.6703 | 16.1829 | 15.7397 | 16.0681 | 18.3124 | 19.6153 | 18.0751 | 18.6471 |
Translational Efficiency | 0.56 | 0.5329 | 0.4564 | 0.4042 | 0.3828 | 0.5462 | 0.3792 | 0.3476 | 0.3911 | 0.3573 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1661 | 1843 | 2493 | 109 | 1620 | 1386 | 1958 | 1891 | 3281 | 3229 | 4451 | 2000 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 677.83 | 834.40 | 957.01 | 1127.75 | 614.41 | 743.14 | 964.46 | 962.22 | 646.52 | 795.23 | 960.29 | 971.24 |
Standard Deviation | 81.18 | 116.23 | 119.07 | 139.09 | 96.54 | 93.88 | 124.65 | 147.06 | 94.57 | 116.34 | 121.62 | 151.38 |
Intensity Change Log 2 | — | 0.299816 | 0.497611 | 0.734452 | — | 0.274432 | 0.650520 | 0.647165 | — | 0.287803 | 0.572336 | 0.693608 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000053 | 0.000115 | 0.000012 | 0.000178 | 0.000068 | 0.000050 | 0.000015 | 0.000036 | 0.000060 | 0.000087 | 0.000013 | 0.000044 |
Bud Neck | 0.011529 | 0.003993 | 0.008177 | 0.014241 | 0.014465 | 0.004136 | 0.006297 | 0.004739 | 0.012979 | 0.004054 | 0.007350 | 0.005257 |
Bud Site | 0.004213 | 0.005842 | 0.003443 | 0.011304 | 0.010157 | 0.005716 | 0.005831 | 0.009898 | 0.007148 | 0.005788 | 0.004494 | 0.009975 |
Cell Periphery | 0.000076 | 0.000083 | 0.000082 | 0.000051 | 0.000171 | 0.000170 | 0.000042 | 0.000144 | 0.000123 | 0.000120 | 0.000064 | 0.000139 |
Cytoplasm | 0.329246* | 0.031088 | 0.006407 | 0.004852 | 0.150926* | 0.015619 | 0.022721 | 0.009410 | 0.241200* | 0.024448 | 0.013583 | 0.009161 |
Cytoplasmic Foci | 0.199030* | 0.046980 | 0.001028 | 0.007633 | 0.121355 | 0.024975 | 0.004710 | 0.006797 | 0.160678 | 0.037535 | 0.002648 | 0.006843 |
Eisosomes | 0.000074 | 0.000017 | 0.000009 | 0.000011 | 0.000061 | 0.000019 | 0.000006 | 0.000008 | 0.000067 | 0.000018 | 0.000008 | 0.000008 |
Endoplasmic Reticulum | 0.003862 | 0.005797 | 0.003794 | 0.001973 | 0.006984 | 0.013610 | 0.001761 | 0.001959 | 0.005404 | 0.009150 | 0.002900 | 0.001960 |
Endosome | 0.065271 | 0.086316 | 0.015465 | 0.052208 | 0.120546 | 0.068559 | 0.025995 | 0.055832 | 0.092563 | 0.078694 | 0.020097 | 0.055634 |
Golgi | 0.002116 | 0.003165 | 0.000467 | 0.003060 | 0.004577 | 0.003814 | 0.001433 | 0.004760 | 0.003331 | 0.003444 | 0.000892 | 0.004667 |
Lipid Particles | 0.011834 | 0.002753 | 0.000326 | 0.000486 | 0.013615 | 0.005515 | 0.000313 | 0.000441 | 0.012713 | 0.003938 | 0.000320 | 0.000443 |
Mitochondria | 0.004270 | 0.007236 | 0.001287 | 0.000437 | 0.009106 | 0.013912 | 0.001188 | 0.002505 | 0.006657 | 0.010102 | 0.001243 | 0.002392 |
Mitotic Spindle | 0.002055 | 0.000984 | 0.000432 | 0.012745 | 0.000551 | 0.000860 | 0.001471 | 0.000944 | 0.001312 | 0.000931 | 0.000889 | 0.001588 |
None | 0.001261 | 0.000165 | 0.000160 | 0.000077 | 0.004416 | 0.000829 | 0.000199 | 0.000463 | 0.002819 | 0.000450 | 0.000177 | 0.000442 |
Nuclear Periphery | 0.001065 | 0.000862 | 0.002010 | 0.003731 | 0.001655 | 0.002097 | 0.001079 | 0.000968 | 0.001356 | 0.001392 | 0.001601 | 0.001118 |
Nuclear Periphery Foci | 0.000597 | 0.000311 | 0.000261 | 0.002162 | 0.001023 | 0.000705 | 0.000287 | 0.000387 | 0.000807 | 0.000480 | 0.000272 | 0.000483 |
Nucleolus | 0.001435 | 0.000641 | 0.005648 | 0.005768 | 0.004073 | 0.001434 | 0.003943 | 0.002541 | 0.002738 | 0.000981 | 0.004898 | 0.002717 |
Nucleus | 0.061048 | 0.004715 | 0.025780 | 0.006757 | 0.037989 | 0.011066 | 0.014445 | 0.005351 | 0.049663 | 0.007441 | 0.020794 | 0.005427 |
Peroxisomes | 0.002595 | 0.000799 | 0.000147 | 0.000814 | 0.002401 | 0.000863 | 0.000340 | 0.000462 | 0.002500 | 0.000827 | 0.000232 | 0.000481 |
Vacuole | 0.290057* | 0.626245* | 0.733254* | 0.698529* | 0.416162* | 0.603623* | 0.725814* | 0.669090* | 0.352322* | 0.616535* | 0.729981* | 0.670694* |
Vacuole Periphery | 0.008314 | 0.171895* | 0.191811* | 0.172984* | 0.079698 | 0.222427* | 0.182110* | 0.223267* | 0.043560 | 0.193585* | 0.187543* | 0.220526* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.34 | 6.12 | -1.32 | -0.71 | -1.68 | 0.80 | 2.68 | 1.50 | 0.85 | -2.09 | -0.92 | 4.57 | 1.03 | 1.44 | -2.91 |
Bud Neck | 7.80 | 2.84 | -0.76 | -2.23 | -1.33 | 9.52 | 7.28 | 8.99 | -0.91 | 2.51 | 12.34 | 6.97 | 8.83 | -2.00 | 3.29 |
Bud Site | -2.24 | 1.05 | -1.92 | -1.54 | -2.07 | 3.59 | 4.63 | 0.06 | -3.21 | -3.86 | 1.92 | 4.70 | -3.01 | -4.16 | -5.79 |
Cell Periphery | -0.91 | -0.31 | 1.81 | 2.12 | 1.31 | 0.03 | 8.37 | 0.99 | 0.95 | -4.54 | 0.21 | 4.58 | -0.73 | -0.83 | -3.19 |
Cytoplasm | 45.91* | 51.38 | 48.12 | 7.77 | -0.03 | 26.58 | 23.44 | 27.98 | 2.41 | 6.15 | 48.91* | 51.51 | 52.15 | 7.89 | 3.08 |
Cytoplasmic Foci | 33.56* | 48.71 | 35.08 | 9.48 | -1.87 | 26.61 | 35.67 | 34.73 | 10.71 | -3.12 | 40.89 | 58.53 | 55.89 | 21.11 | -7.19 |
Eisosomes | 15.03 | 20.11 | 14.66 | 1.37 | -1.02 | 10.10 | 14.03 | 13.46 | 7.22 | -3.26 | 17.48 | 23.56 | 23.09 | 6.75 | -0.63 |
Endoplasmic Reticulum | -3.81 | -0.04 | 1.81 | 3.91 | 1.95 | -5.41 | 8.73 | 8.28 | 10.64 | -0.87 | -6.11 | 6.82 | 9.06 | 13.31 | 5.22 |
Endosome | -5.98 | 20.10 | 1.34 | 3.61 | -4.11 | 11.66 | 27.45 | 16.14 | 3.44 | -11.72 | 4.69 | 33.52 | 12.06 | 7.75 | -15.32 |
Golgi | -1.85 | 3.58 | -0.57 | 0.05 | -1.59 | 0.87 | 5.86 | -0.22 | -1.01 | -5.39 | -0.26 | 6.91 | -1.96 | -1.77 | -6.44 |
Lipid Particles | 12.00 | 16.21 | 15.58 | 7.54 | -1.92 | 6.75 | 13.07 | 12.90 | 7.94 | -1.34 | 12.66 | 20.04 | 19.65 | 10.88 | -1.48 |
Mitochondria | -2.68 | 3.23 | 4.25 | 9.01 | 2.32 | -3.31 | 11.12 | 8.78 | 8.62 | -4.05 | -3.93 | 9.22 | 6.87 | 10.36 | -3.51 |
Mitotic Spindle | 1.15 | 1.82 | -1.79 | -1.92 | -1.97 | -0.93 | -2.11 | -1.59 | -0.34 | 1.07 | 0.75 | 0.67 | -0.90 | -1.64 | -1.59 |
None | 2.99 | 3.02 | 3.20 | 1.23 | 1.69 | 3.39 | 4.12 | 3.81 | 1.14 | -1.39 | 4.30 | 4.90 | 4.21 | 0.05 | -1.52 |
Nuclear Periphery | 0.86 | -3.70 | -1.27 | -1.35 | -0.83 | -1.03 | 1.86 | 1.84 | 2.39 | 0.57 | -0.17 | -1.74 | 0.58 | 0.64 | 1.66 |
Nuclear Periphery Foci | 3.93 | 3.92 | -1.41 | -1.54 | -1.55 | 1.47 | 5.67 | 4.30 | 1.52 | -1.23 | 3.13 | 6.83 | 1.39 | -0.69 | -2.06 |
Nucleolus | 4.10 | -11.09 | -1.58 | -1.82 | -0.29 | 5.49 | -0.08 | 2.43 | -2.35 | 2.60 | 6.97 | -6.73 | -0.17 | -3.95 | 4.22 |
Nucleus | 25.05 | 12.05 | 17.03 | -1.36 | 6.76 | 9.38 | 8.15 | 12.88 | 3.22 | 7.44 | 23.41 | 13.75 | 25.05 | 1.72 | 12.73 |
Peroxisomes | 5.51 | 7.86 | 3.79 | -0.14 | -2.06 | 5.64 | 8.32 | 7.88 | 2.95 | -1.68 | 7.88 | 11.38 | 9.95 | 3.73 | -4.50 |
Vacuole | -42.27* | -62.99* | -15.79 | -2.47 | 1.73 | -19.47 | -38.32 | -29.97 | -7.48 | 7.88 | -42.15* | -69.29* | -46.60 | -7.96 | 9.75 |
Vacuole Periphery | -39.25* | -49.80* | -8.06 | 0.17 | 1.09 | -22.25* | -20.22 | -25.24* | 0.09 | -7.04 | -39.64 | -45.62 | -35.51 | -4.38 | -6.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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