Standard name | |
---|---|
Human Ortholog | |
Description | Beta subunit of the oligosaccharyl transferase glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum; human homolog DDOST can complement yeast growth defect during down-regulation of yeast gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 19 | 57 | 47 | 69 | 96 | 140 | 98 | – | 158 | 132 | 178 | 17 | 25 | 26 | 25 | 62 | 15 | 0 | 5 | 18 | 14 | 16 | 13 |
Bud | 0 | 4 | 3 | 0 | 2 | 4 | 11 | 7 | – | 13 | 19 | 10 | 1 | 0 | 3 | 6 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 36 | 22 | 33 | 36 | 71 | 69 | – | 103 | 86 | 132 | 27 | 46 | 51 | 33 | 94 | 54 | 0 | 0 | 8 | 5 | 21 | 25 |
Cytoplasm | 4 | 33 | 14 | 17 | 25 | 30 | 26 | 31 | – | 39 | 45 | 53 | 29 | 17 | 29 | 22 | 47 | 37 | 0 | 9 | 0 | 0 | 4 | 1 |
Endoplasmic Reticulum | 70 | 235 | 500 | 297 | 490 | 664 | 849 | 982 | – | 772 | 384 | 622 | 436 | 446 | 394 | 1 | 3 | 8 | 90 | 261 | 498 | 292 | 528 | 612 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 4 | 3 | 73 | 129 | 248 | 106 | 142 | 101 | – | 48 | 46 | 53 | 30 | 36 | 24 | 12 | 50 | 35 | 0 | 2 | 0 | 6 | 8 | 7 |
Mitochondria | 3 | 4 | 15 | 17 | 27 | 63 | 72 | 57 | – | 184 | 152 | 163 | 0 | 0 | 0 | 17 | 35 | 20 | 0 | 1 | 2 | 4 | 7 | 6 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 1 | 4 | 4 | 9 | 30 | 41 | – | 52 | 24 | 37 | 5 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 0 | 2 | 5 | 34 | 11 | 19 | 23 | – | 18 | 27 | 10 | 1 | 5 | 20 | 3 | 3 | 1 | 0 | 3 | 1 | 1 | 2 | 4 |
Unique Cell Count | 76 | 272 | 534 | 372 | 625 | 759 | 991 | 1127 | 986 | 616 | 870 | 469 | 481 | 461 | 97 | 240 | 143 | 95 | 288 | 537 | 328 | 594 | 679 | |
Labelled Cell Count | 95 | 309 | 701 | 538 | 932 | 1020 | 1360 | 1411 | 1389 | 916 | 1259 | 546 | 577 | 549 | 120 | 297 | 171 | 95 | 288 | 537 | 328 | 594 | 679 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.5 | 26.5 | 23.3 | 23.7 | 23.3 | 20.1 | 18.9 | 19.4 | – | 16.5 | 16.2 | 15.9 | 26.2 | 26.0 | 25.9 | 16.3 | 20.0 | 22.5 | 27.0 | 28.1 | 29.8 |
Std Deviation (1e-4) | 4.5 | 5.2 | 4.0 | 3.9 | 3.8 | 3.6 | 3.2 | 3.7 | – | 3.2 | 3.2 | 2.8 | 4.2 | 4.4 | 6.3 | 3.7 | 5.3 | 6.6 | 4.2 | 4.9 | 5.2 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.0 | -0.21 | -0.3 | -0.27 | – | -0.49 | -0.52 | -0.55 | 0.17 | 0.16 | 0.15 | -0.51 | -0.22 | -0.05 | 0.21 | 0.27 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.9 | 0.2 | 1.1 | 1.9 | -1.3 | – | 2.9 | 4.9 | 4.8 | -4.3 | -3.2 | -2.9 | 4.1 | 5.4 | -0.1 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 1.4 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -1.0 | -1.5 | 0.3 | -0.5 | – | 2.4 | 4.0 | 4.7 | -0.6 | 1.6 | 2.4 | 7.9 | 11.2 | 9.7 |
Cytoplasm | 1.6 | 1.3 | 1.3 | 0 | 0.2 | – | 1.4 | 3.6 | 3.0 | 2.8 | 0.8 | 2.8 | 7.8 | 8.1 | 9.4 |
Endoplasmic Reticulum | -6.3 | -7.3 | -3.6 | -4.6 | -4.0 | – | -7.7 | -12.6 | -10.1 | -0.4 | -0.6 | -4.3 | -20.7 | -24.9 | -21.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 7.5 | 9.9 | 0.2 | 0.4 | -2.9 | – | -6.1 | -3.4 | -4.8 | -3.8 | -3.2 | -4.5 | -0.3 | 2.5 | 3.1 |
Mitochondria | 1.4 | 1.4 | 4.1 | 3.6 | 2.1 | – | 8.7 | 10.5 | 8.7 | -3.7 | -3.7 | -3.6 | 0 | 6.2 | 5.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 3.7 | 4.2 | – | 5.2 | 4.3 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 5.0 | 1.9 | 2.5 | 2.6 | – | 2.4 | 4.3 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.1042 | 18.6755 | 18.6695 | 6.9116 | 15.9994 | 14.7278 | 20.5043 | 21.963 | 21.8974 | 19.1021 | 19.4589 | 21.195 | 23.9225 | 22.3705 | 23.7706 | 21.463 | 22.8003 | 23.0478 |
Actin | 0.0515 | 0.0032 | 0.004 | 0.1146 | 0.0042 | 0.0604 | 0.013 | 0.001 | 0.0176 | 0.0058 | 0.018 | 0.0109 | 0.0056 | 0.0238 | 0.0034 | 0.007 | 0.0009 | 0.0004 |
Bud | 0.0028 | 0.0001 | 0.0001 | 0.0015 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0014 | 0.0008 | 0.002 | 0.0087 | 0.0036 | 0.0077 | 0.0006 | 0.0007 | 0.002 | 0.0016 | 0.0009 | 0.0014 | 0.0003 | 0.0009 | 0.0005 | 0.0023 | 0.0008 | 0.0006 |
Bud Periphery | 0.0028 | 0.0003 | 0.0006 | 0.0018 | 0.001 | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0 |
Bud Site | 0.0155 | 0.0009 | 0.0007 | 0.0053 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0.0163 | 0.0001 | 0.0001 | 0.0003 | 0.0024 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0.0059 | 0.0149 | 0.0101 | 0.0009 | 0.0053 | 0.0048 | 0.0034 | 0.0189 | 0.0076 | 0.0035 | 0.0019 | 0.0027 | 0.0054 | 0.0158 | 0.0062 | 0.0042 | 0.004 | 0.0016 |
Cytoplasm | 0.0447 | 0.0938 | 0.0826 | 0.1568 | 0.046 | 0.0496 | 0.0262 | 0.0489 | 0.0116 | 0.0429 | 0.0048 | 0.0058 | 0.0079 | 0.0498 | 0.0197 | 0.006 | 0.0034 | 0.0055 |
Cytoplasmic Foci | 0.0162 | 0.0027 | 0.0014 | 0.047 | 0.0098 | 0.0046 | 0.0117 | 0.0024 | 0.0034 | 0.0178 | 0.0099 | 0.0066 | 0.0015 | 0.0021 | 0.0018 | 0.0002 | 0.0011 | 0.0006 |
Eisosomes | 0.0013 | 0.0003 | 0.0001 | 0.0011 | 0 | 0.0004 | 0.0005 | 0.0029 | 0.0008 | 0.0001 | 0.0002 | 0.0011 | 0.0028 | 0.0008 | 0.0005 | 0.0058 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.5822 | 0.7931 | 0.8783 | 0.2228 | 0.7099 | 0.6361 | 0.8285 | 0.8506 | 0.8742 | 0.8258 | 0.8672 | 0.9182 | 0.9244 | 0.8372 | 0.923 | 0.9462 | 0.9452 | 0.9739 |
Endosome | 0.0335 | 0.004 | 0.0018 | 0.0763 | 0.0375 | 0.0426 | 0.0184 | 0.0101 | 0.0092 | 0.0255 | 0.0074 | 0.0045 | 0.0095 | 0.0085 | 0.0066 | 0.0026 | 0.0065 | 0.0023 |
Golgi | 0.013 | 0.0166 | 0.0019 | 0.0155 | 0.0034 | 0.0418 | 0.0043 | 0.014 | 0.0148 | 0.0063 | 0.0153 | 0.0087 | 0.0098 | 0.0129 | 0.0179 | 0.0174 | 0.0223 | 0.0065 |
Lipid Particles | 0.0431 | 0.0262 | 0.0061 | 0.0106 | 0.0067 | 0.0507 | 0.0072 | 0.0141 | 0.0079 | 0.0073 | 0.0092 | 0.0077 | 0.0061 | 0.0125 | 0.0019 | 0.0008 | 0.003 | 0.0012 |
Mitochondria | 0.0066 | 0.0002 | 0.0001 | 0.0557 | 0.0011 | 0.0112 | 0.0007 | 0.0149 | 0.0226 | 0.0089 | 0.0008 | 0.0044 | 0.0031 | 0.0036 | 0.0007 | 0.0008 | 0.0007 | 0.0003 |
None | 0.0083 | 0.0087 | 0.0001 | 0.0898 | 0.0022 | 0.0008 | 0.0028 | 0.0006 | 0.0004 | 0.002 | 0.0002 | 0.0004 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0321 | 0.0092 | 0.0064 | 0.0685 | 0.0177 | 0.0389 | 0.0581 | 0.011 | 0.0113 | 0.0161 | 0.0403 | 0.0185 | 0.0167 | 0.0182 | 0.0127 | 0.0043 | 0.0089 | 0.0055 |
Nucleolus | 0.0018 | 0 | 0 | 0.0161 | 0.0014 | 0.0001 | 0.002 | 0.0002 | 0.0008 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Nucleus | 0.0475 | 0.0008 | 0.0005 | 0.059 | 0.0043 | 0.0304 | 0.0087 | 0.0007 | 0.0026 | 0.0016 | 0.0006 | 0.0024 | 0.0005 | 0.0014 | 0.0008 | 0.0002 | 0.0003 | 0.0002 |
Peroxisomes | 0.0616 | 0.0182 | 0 | 0.0076 | 0.0002 | 0.0075 | 0.0014 | 0.0001 | 0.0002 | 0.0017 | 0.0204 | 0.0017 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0139 | 0.0003 | 0.0002 | 0.0236 | 0.0005 | 0.003 | 0.0056 | 0.0001 | 0.0055 | 0.0006 | 0.0003 | 0.0011 | 0.0005 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.011 | 0.0055 | 0.0028 | 0.0047 | 0.1337 | 0.0045 | 0.0037 | 0.0065 | 0.0044 | 0.0094 | 0.001 | 0.0019 | 0.0022 | 0.0065 | 0.0029 | 0.0014 | 0.0015 | 0.0009 |
Vacuole Periphery | 0.0034 | 0.0004 | 0.0002 | 0.0122 | 0.0105 | 0.0029 | 0.0025 | 0.0014 | 0.0022 | 0.0029 | 0.0013 | 0.0014 | 0.0018 | 0.0021 | 0.0009 | 0.0005 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 139.5256 | 135.7888 | 95.1004 | 129.6832 | 120.0475 | 104.0532 | 125.1476 | 115.8319 | 109.9209 | 147.6316 |
Translational Efficiency | 1.3011 | 1.1913 | 1.5113 | 1.0791 | 1.2463 | 1.9624 | 1.2255 | 1.1838 | 1.3371 | 1.2243 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
99 | 744 | 1944 | 298 | 1760 | 725 | 2451 | 1387 | 1859 | 1469 | 4395 | 1685 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1813.37 | 1802.53 | 2412.38 | 2269.18 | 1675.38 | 1806.06 | 2304.37 | 2193.22 | 1682.73 | 1804.27 | 2352.15 | 2206.65 |
Standard Deviation | 256.04 | 438.28 | 376.95 | 376.88 | 287.41 | 318.53 | 341.47 | 412.76 | 287.50 | 383.88 | 361.60 | 407.68 |
Intensity Change Log 2 | — | -0.008650 | 0.411784 | 0.323498 | — | 0.108357 | 0.459884 | 0.388562 | — | 0.048725 | 0.435083 | 0.355110 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000757 | 0.003599 | 0.000381 | 0.000405 | 0.001809 | 0.002470 | 0.001209 | 0.001528 | 0.001753 | 0.003042 | 0.000843 | 0.001329 |
Bud Neck | 0.002348 | 0.000585 | 0.000368 | 0.002022 | 0.000178 | 0.000258 | 0.000545 | 0.001836 | 0.000294 | 0.000423 | 0.000466 | 0.001869 |
Bud Site | 0.006025 | 0.003631 | 0.000530 | 0.002945 | 0.000748 | 0.001665 | 0.000953 | 0.005834 | 0.001029 | 0.002661 | 0.000766 | 0.005323 |
Cell Periphery | 0.028682 | 0.008839 | 0.003693 | 0.043024 | 0.006247 | 0.002554 | 0.004874 | 0.015240 | 0.007442 | 0.005737 | 0.004352 | 0.020153 |
Cytoplasm | 0.001650 | 0.001318 | 0.000081 | 0.000169 | 0.000214 | 0.001285 | 0.000541 | 0.004014 | 0.000291 | 0.001302 | 0.000337 | 0.003334 |
Cytoplasmic Foci | 0.006259 | 0.004409 | 0.000049 | 0.001046 | 0.000492 | 0.002738 | 0.000722 | 0.004107 | 0.000800 | 0.003584 | 0.000424 | 0.003565 |
Eisosomes | 0.000212 | 0.000692 | 0.000501 | 0.000889 | 0.000486 | 0.000202 | 0.000504 | 0.000521 | 0.000471 | 0.000450 | 0.000503 | 0.000586 |
Endoplasmic Reticulum | 0.861301* | 0.904632* | 0.981629* | 0.840811* | 0.974007* | 0.960967* | 0.950001* | 0.858333* | 0.968005* | 0.932435* | 0.963991* | 0.855234* |
Endosome | 0.014281 | 0.015526 | 0.002336 | 0.008166 | 0.003425 | 0.009566 | 0.006902 | 0.015521 | 0.004003 | 0.012585 | 0.004882 | 0.014220 |
Golgi | 0.009196 | 0.022218 | 0.003592 | 0.024886 | 0.005200 | 0.006456 | 0.009491 | 0.018639 | 0.005413 | 0.014439 | 0.006882 | 0.019744 |
Lipid Particles | 0.037635 | 0.022118 | 0.002413 | 0.041472 | 0.003520 | 0.005415 | 0.015834 | 0.044417 | 0.005336 | 0.013875 | 0.009898 | 0.043896 |
Mitochondria | 0.000097 | 0.000367 | 0.000100 | 0.001731 | 0.000620 | 0.000336 | 0.000598 | 0.000513 | 0.000592 | 0.000352 | 0.000378 | 0.000728 |
Mitotic Spindle | 0.010679 | 0.000122 | 0.000015 | 0.002615 | 0.000307 | 0.000302 | 0.000054 | 0.002185 | 0.000859 | 0.000211 | 0.000037 | 0.002261 |
None | 0.000006 | 0.001053 | 0.000360 | 0.000054 | 0.000792 | 0.000180 | 0.000650 | 0.000291 | 0.000750 | 0.000622 | 0.000522 | 0.000249 |
Nuclear Periphery | 0.000812 | 0.000472 | 0.000878 | 0.002386 | 0.000251 | 0.000298 | 0.000867 | 0.001393 | 0.000281 | 0.000386 | 0.000872 | 0.001568 |
Nuclear Periphery Foci | 0.006653 | 0.003726 | 0.000482 | 0.003995 | 0.000264 | 0.001097 | 0.001524 | 0.009505 | 0.000604 | 0.002429 | 0.001063 | 0.008531 |
Nucleolus | 0.000003 | 0.000005 | 0.000002 | 0.000007 | 0.000005 | 0.000079 | 0.000002 | 0.000014 | 0.000005 | 0.000042 | 0.000002 | 0.000012 |
Nucleus | 0.000091 | 0.000060 | 0.000024 | 0.000060 | 0.000035 | 0.001075 | 0.000034 | 0.000238 | 0.000038 | 0.000561 | 0.000030 | 0.000206 |
Peroxisomes | 0.000152 | 0.000305 | 0.000012 | 0.000080 | 0.000272 | 0.000161 | 0.000494 | 0.000140 | 0.000266 | 0.000234 | 0.000281 | 0.000130 |
Vacuole | 0.012029 | 0.004024 | 0.002059 | 0.019509 | 0.000754 | 0.001990 | 0.003342 | 0.012883 | 0.001355 | 0.003020 | 0.002775 | 0.014055 |
Vacuole Periphery | 0.001134 | 0.002299 | 0.000493 | 0.003729 | 0.000373 | 0.000905 | 0.000859 | 0.002851 | 0.000414 | 0.001611 | 0.000697 | 0.003006 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.85 | 0.69 | 0.70 | 2.15 | -0.00 | -0.54 | 0.72 | 0.22 | 0.81 | -0.87 | -1.15 | 1.22 | 0.45 | 1.76 | -1.64 |
Bud Neck | 1.80 | 2.00 | -0.02 | -2.10 | -2.33 | -0.87 | -1.88 | -3.95 | -3.75 | -2.94 | -1.31 | -1.28 | -4.29 | -4.02 | -3.79 |
Bud Site | 0.94 | 2.20 | 0.93 | 0.11 | -2.21 | -1.99 | -0.92 | -5.65 | -4.41 | -5.42 | -3.39 | 0.80 | -5.56 | -3.36 | -5.97 |
Cell Periphery | 2.70 | 3.44 | -1.45 | -4.98 | -5.81 | 3.50 | 1.22 | -5.09 | -8.63 | -6.86 | 1.38 | 2.81 | -6.36 | -7.90 | -9.12 |
Cytoplasm | 0.57 | 2.97 | 2.80 | 4.66 | -1.56 | -1.64 | -2.96 | -4.88 | -2.96 | -4.49 | -2.87 | -0.74 | -4.76 | -3.08 | -4.68 |
Cytoplasmic Foci | 0.50 | 1.71 | 1.42 | 2.75 | -2.11 | -2.32 | -1.23 | -5.56 | -0.81 | -5.20 | -3.58 | 1.52 | -4.77 | 0.17 | -5.85 |
Eisosomes | -3.13 | -1.76 | -2.49 | -0.65 | -1.25 | 3.04 | -0.16 | -0.16 | -1.55 | -0.07 | 0.19 | -0.28 | -0.60 | -0.72 | -0.43 |
Endoplasmic Reticulum | -1.72 | -4.88 | 0.75 | 3.93 | 9.31 | 2.58 | 7.00 | 16.22 | 12.42 | 12.64 | 7.21 | 1.45 | 16.92 | 10.46 | 16.99 |
Endosome | -0.30 | 3.58 | 1.76 | 4.23 | -5.71 | -4.79 | -7.38 | -12.22 | -3.87 | -8.15 | -8.74 | -2.47 | -11.86 | -1.47 | -10.88 |
Golgi | -2.60 | 1.27 | -2.60 | -0.54 | -5.11 | -1.05 | -5.55 | -7.79 | -6.03 | -5.08 | -6.18 | -2.48 | -8.81 | -2.53 | -7.97 |
Lipid Particles | 1.62 | 3.76 | -0.36 | -2.80 | -5.97 | -1.48 | -8.24 | -11.98 | -10.88 | -7.83 | -5.10 | -4.26 | -12.49 | -8.97 | -10.91 |
Mitochondria | -1.93 | -0.25 | -1.05 | -0.87 | -1.04 | 1.03 | 0.06 | 0.32 | -1.04 | 0.20 | 0.95 | 0.71 | -0.38 | -1.24 | -1.02 |
Mitotic Spindle | 1.31 | 1.32 | 0.95 | -1.47 | -1.53 | -0.02 | 1.17 | -2.10 | -2.11 | -2.41 | 1.32 | 1.72 | -1.64 | -2.60 | -2.82 |
None | -1.32 | -2.03 | -4.67 | 1.26 | 1.76 | 1.83 | 0.40 | 1.47 | -1.46 | 2.21 | 0.26 | 0.69 | 1.57 | 0.91 | 2.17 |
Nuclear Periphery | 1.72 | -0.33 | -4.80 | -7.16 | -5.56 | -0.66 | -5.11 | -10.81 | -8.68 | -3.32 | -2.28 | -7.96 | -12.90 | -11.05 | -5.71 |
Nuclear Periphery Foci | 1.37 | 3.05 | 1.02 | -0.26 | -2.46 | -3.60 | -3.98 | -6.33 | -5.73 | -5.38 | -4.10 | -2.17 | -6.43 | -4.80 | -6.04 |
Nucleolus | -1.22 | 1.01 | -2.16 | -1.44 | -4.17 | -1.20 | 1.08 | -2.32 | 1.06 | -3.61 | -1.20 | 1.20 | -2.30 | 0.95 | -3.95 |
Nucleus | 0.99 | 1.90 | 0.92 | -0.10 | -2.23 | -1.16 | -0.17 | -3.76 | 0.86 | -3.76 | -1.18 | 0.53 | -3.78 | 0.67 | -3.87 |
Peroxisomes | -1.27 | 2.71 | 1.07 | 1.93 | -1.58 | 0.86 | -1.56 | 2.32 | 0.60 | 2.63 | 0.22 | -0.16 | 2.55 | 1.67 | 1.96 |
Vacuole | 2.74 | 3.49 | -1.69 | -4.33 | -4.94 | -1.78 | -5.77 | -9.11 | -7.36 | -6.92 | -3.22 | -4.31 | -9.99 | -8.30 | -8.82 |
Vacuole Periphery | -1.15 | 1.21 | -2.12 | -1.02 | -2.92 | -1.77 | -2.57 | -4.67 | -3.21 | -3.61 | -2.57 | -2.20 | -5.39 | -2.11 | -4.76 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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