Standard name
Human Ortholog
Description WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.95 0.81 0.58 0.73 0.71 – 0.64 0.76 0.62 0.66 0.93 0.95 0.9 0.93 0.86 0.9 0.86 0.74 0.81 0.87 0.75 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.05 0 0 – 0.07 0.05 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0.07 0.06 0 0.05 0.05 – 0.06 0.07 0.05 0.07 0 0 0 0 0 0 0.08 0.13 0.13 0.06 0.09 0.11
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0.21 0.41 0.2 0.25 – 0.26 0.11 0.22 0.15 0.1 0.11 0.14 0.1 0.18 0.1 0 0.07 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 2 – 2 7 7 12 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 1 0 – 1 0 1 3 0 0 0 0 0 0 3 1 2 2 13 11
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 5
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 3 2 – 5 2 10 13 1 1 0 1 1 1 0 0 0 0 0 0
Cytoplasm 351 75 107 153 130 177 288 – 163 210 215 302 293 411 427 144 124 194 319 72 108 166 364 281
Endoplasmic Reticulum 4 1 2 2 6 4 13 – 7 10 7 27 7 8 6 8 7 11 2 0 0 2 9 9
Endosome 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 2 1 0 0 2 2
Golgi 0 0 0 0 12 5 15 – 19 15 35 32 2 0 0 0 2 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 3 1 – 0 2 6 11 0 0 0 0 9 4 0 0 0 0 5 0
Nucleus 1 2 8 12 3 13 22 – 15 19 18 32 1 3 5 4 2 2 30 12 17 11 45 39
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 1 0 1 0 6 3
Nucleolus 0 0 0 0 0 1 0 – 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 2
Vac/Vac Membrane 20 1 5 40 92 49 100 – 66 31 77 67 30 47 67 16 26 22 3 7 1 4 22 15
Unique Cell Count 367 77 113 188 224 244 405 254 278 344 459 315 434 476 155 144 216 372 98 134 191 484 379
Labelled Cell Count 378 79 122 207 243 258 443 278 296 377 500 334 470 506 174 171 238 372 98 134 191 484 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.2 22.1 23.1 22.6 24.3 18.0 23.1 – 21.7 21.0 20.9 21.1 26.2 24.1 26.3 31.7 31.1 31.0 23.5 22.4 24.0
Std Deviation (1e-4) 5.0 4.2 4.1 4.5 4.9 4.7 5.2 – 5.2 5.1 5.7 5.1 5.1 5.6 5.7 8.6 10.0 10.3 4.9 5.6 5.3
Intensity Change (Log2) – – – -0.03 0.08 -0.36 0.0 – -0.09 -0.14 -0.14 -0.13 0.18 0.06 0.19 0.46 0.43 0.43 0.03 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.3 -7.0 -4.8 -5.2 – -6.1 -4.4 -6.5 -6.1 -0.6 0 -1.6 -0.6 -2.3 -1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 1.8 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 3.0 0 3.5 2.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -0.2 0 -0.7 -0.7 – -0.4 -0.1 -0.7 0 0 0 0 -1.8 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 4.0 7.0 3.8 4.7 – 4.8 2.1 4.3 2.9 1.7 2.1 2.8 1.8 3.3 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.4486 13.9597 13.9495 13.3321 12.8696 14.0491 16.8904 20.6378 18.1925 17.4247 14.3031 18.0826 17.3384 21.6909 20.0368 19.6379 18.9212 19.8934
Actin 0.014 0.0004 0.001 0.0003 0.0002 0.0003 0.0307 0.0003 0.0089 0.0053 0.0406 0.0117 0.0208 0.0007 0.0015 0.0424 0.0178 0.0002
Bud 0.0004 0.0003 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001 0.0007 0.0006 0.0007 0.0002 0.0006 0.0002 0.0001 0.007 0.0003 0.0001
Bud Neck 0.0012 0.0005 0.0005 0.0006 0.0008 0.0019 0.0052 0.0004 0.0008 0.0013 0.0023 0.0018 0.0007 0.0005 0.0007 0.0015 0.0016 0.0015
Bud Periphery 0.0005 0.0001 0.0001 0 0 0 0.001 0 0.0007 0.0004 0.0009 0.0003 0.0007 0 0 0.0045 0.0003 0
Bud Site 0.0006 0.0124 0.0021 0 0 0.0001 0.006 0.0002 0.0029 0.0007 0.0012 0.0003 0.0023 0.0007 0.0007 0.0066 0.003 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0007 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0.0002 0.0001 0
Cytoplasm 0.4702 0.4975 0.4427 0.3905 0.5191 0.3867 0.5163 0.6477 0.5387 0.5131 0.534 0.4884 0.5243 0.6629 0.5067 0.404 0.5561 0.4997
Cytoplasmic Foci 0.0108 0.0004 0.0036 0.0002 0.0002 0.0089 0.0213 0.0003 0.0075 0.013 0.0301 0.0022 0.0209 0.0006 0.0017 0.0107 0.0015 0.0002
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0.0003 0.0003 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0062 0.002 0.0035 0.0006 0.0017 0.0014 0.0025 0.0005 0.0039 0.0073 0.006 0.0032 0.0073 0.0013 0.0006 0.0007 0.001 0.0009
Endosome 0.0089 0.0004 0.0039 0.0001 0.0001 0.0009 0.0077 0.0001 0.0162 0.0201 0.0427 0.0109 0.0147 0.0003 0.0008 0.0032 0.0006 0.0002
Golgi 0.0017 0 0.0015 0 0 0.0001 0.0065 0 0.0051 0.0123 0.0526 0.0021 0.0067 0 0.0018 0.0029 0.0018 0
Lipid Particles 0.0022 0 0.0002 0 0 0.0003 0.0068 0 0.0018 0.0025 0.0325 0.0037 0.0064 0 0.0013 0.0039 0.0017 0
Mitochondria 0.0014 0.0001 0.0004 0.0001 0.0001 0.0002 0.0173 0.0002 0.0158 0.0026 0.0241 0.0076 0.0079 0.0003 0.0014 0.0124 0.0019 0.0002
None 0.0035 0.0003 0.001 0.0003 0.0002 0.0003 0.002 0.0002 0.0008 0.0013 0.0011 0.0008 0.0032 0.0003 0.0002 0.0004 0.0002 0.0002
Nuclear Periphery 0.0324 0.0194 0.0226 0.0155 0.0167 0.0225 0.0177 0.0116 0.0158 0.0181 0.0201 0.0199 0.0218 0.0122 0.0125 0.0115 0.0141 0.0123
Nucleolus 0.0009 0.0002 0.0002 0.0001 0.0001 0.0002 0.0006 0.0003 0.0003 0.0002 0.0005 0.0006 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001
Nucleus 0.4351 0.4632 0.5125 0.5891 0.459 0.5681 0.3429 0.3349 0.3704 0.3935 0.1839 0.4402 0.3459 0.3172 0.4663 0.4489 0.3901 0.4824
Peroxisomes 0.0015 0 0.0002 0 0 0.0004 0.0049 0 0.001 0.002 0.0075 0.0009 0.0031 0.0001 0.0016 0.0358 0.0053 0
Punctate Nuclear 0.005 0.0017 0.0028 0.0022 0.0013 0.0067 0.0065 0.0026 0.0056 0.004 0.0152 0.0024 0.0072 0.0021 0.0016 0.0019 0.0021 0.0016
Vacuole 0.0026 0.0007 0.0006 0.0002 0.0003 0.0008 0.0016 0.0002 0.0019 0.0012 0.002 0.0017 0.0026 0.0003 0.0002 0.0011 0.0003 0.0003
Vacuole Periphery 0.0007 0.0001 0.0002 0.0001 0.0001 0.0002 0.0008 0.0001 0.0009 0.0005 0.0015 0.0008 0.0018 0.0001 0.0001 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 127.5161 125.6234 131.1957 123.6801 149.5844 164.7255 145.4431 149.2309 161.3192 138.6316
Translational Efficiency 3.0471 2.8989 2.4086 2.2646 2.5905 2.4968 2.2524 2.3193 2.0372 2.079

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
131 1245 630 987 1866 1763 134 1605 1997 3008 764 2592

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2063.50 1784.17 1689.12 1843.53 1647.22 1758.94 1889.98 1923.34 1674.53 1769.38 1724.35 1892.95
Standard Deviation 409.06 347.02 303.86 474.41 279.69 318.13 432.24 554.00 307.72 330.63 338.74 526.55
Intensity Change Log 2 — -0.209840 -0.288822 -0.162623 — 0.094673 0.198338 0.223581 — -0.066683 -0.052102 0.021667

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003587 0.000420 0.000494 0.001240 0.000434 0.000381 0.001960 0.000569 0.000641 0.000397 0.000751 0.000825
Bud Neck 0.016893 0.002502 0.006046 0.007879 0.002472 0.004743 0.006067 0.006210 0.003418 0.003816 0.006050 0.006845
Bud Site 0.040468 0.004078 0.003647 0.011066 0.003345 0.007535 0.010218 0.009609 0.005780 0.006104 0.004799 0.010164
Cell Periphery 0.005227 0.000269 0.000353 0.000574 0.000313 0.000211 0.000973 0.000446 0.000635 0.000235 0.000461 0.000494
Cytoplasm 0.352467* 0.389002* 0.193485* 0.315905* 0.450103* 0.491015* 0.250872* 0.380168* 0.443698* 0.448793* 0.203550* 0.355698*
Cytoplasmic Foci 0.048103 0.006464 0.002911 0.017747 0.008148 0.006230 0.013009 0.007598 0.010769 0.006327 0.004682 0.011462
Eisosomes 0.000070 0.000005 0.000010 0.000029 0.000009 0.000011 0.000117 0.000012 0.000013 0.000008 0.000029 0.000018
Endoplasmic Reticulum 0.003736 0.000516 0.000893 0.001368 0.001353 0.000906 0.006685 0.000716 0.001509 0.000745 0.001908 0.000964
Endosome 0.000894 0.000307 0.000243 0.019124 0.000352 0.000562 0.003246 0.002283 0.000387 0.000457 0.000770 0.008696
Golgi 0.000770 0.000333 0.000116 0.033393 0.000171 0.001473 0.000321 0.001090 0.000210 0.001002 0.000152 0.013391
Lipid Particles 0.004026 0.000319 0.000550 0.001745 0.000490 0.000349 0.003035 0.000659 0.000722 0.000337 0.000986 0.001072
Mitochondria 0.000857 0.000365 0.000855 0.002440 0.000343 0.000379 0.000816 0.001080 0.000377 0.000373 0.000848 0.001598
Mitotic Spindle 0.015615 0.003863 0.001180 0.090016 0.003497 0.005059 0.006624 0.032097 0.004292 0.004564 0.002135 0.054152
None 0.003915 0.012150 0.007466 0.016444 0.007941 0.011273 0.003218 0.018824 0.007677 0.011636 0.006721 0.017918
Nuclear Periphery 0.000475 0.000243 0.000810 0.001787 0.000381 0.000359 0.001294 0.000715 0.000387 0.000311 0.000895 0.001123
Nuclear Periphery Foci 0.004324 0.001012 0.000652 0.005673 0.002556 0.000656 0.004671 0.000988 0.002672 0.000803 0.001357 0.002772
Nucleolus 0.000127 0.000153 0.000120 0.000415 0.000092 0.000120 0.000383 0.000220 0.000095 0.000134 0.000166 0.000294
Nucleus 0.462862* 0.570409* 0.763081* 0.416582* 0.505998* 0.457553* 0.425710* 0.508645* 0.503168* 0.504263* 0.703908* 0.473589*
Peroxisomes 0.000125 0.000039 0.000035 0.000382 0.000037 0.000261 0.000392 0.000436 0.000043 0.000169 0.000098 0.000416
Vacuole 0.035200 0.006909 0.016995 0.052922 0.011749 0.010841 0.260181* 0.025858 0.013287 0.009214 0.059648 0.036164
Vacuole Periphery 0.000256 0.000639 0.000059 0.003267 0.000219 0.000084 0.000209 0.001779 0.000221 0.000314 0.000086 0.002345

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.94 4.41 3.97 -3.78 -1.49 1.54 -7.73 -2.95 -4.24 6.82 3.70 -4.26 -1.55 -5.46 2.71
Bud Neck 6.47 4.29 4.23 -8.50 -0.41 -4.04 -7.03 -9.81 -3.74 1.87 -0.97 -6.68 -9.53 -7.66 0.62
Bud Site 5.74 5.57 4.55 -6.46 -6.26 -3.59 -6.35 -7.53 -1.80 1.48 -0.24 -0.93 -5.73 -4.90 -5.12
Cell Periphery 6.49 6.09 6.04 -7.07 -0.68 4.80 -7.27 -7.16 -11.34 4.37 6.41 -0.66 0.36 -13.00 1.44
Cytoplasm -3.61 6.21 2.40 13.22 -8.68 -1.75 15.99 15.54 16.52 -8.05 1.38 33.08 20.11 20.54 -16.24
Cytoplasmic Foci 5.11 5.64 5.47 2.05 -1.59 1.21 0.02 3.11 1.05 1.52 3.42 6.84 6.02 2.06 -1.50
Eisosomes 4.60 3.74 2.96 -10.92 -4.49 -2.25 -8.49 -10.25 -7.15 6.80 3.53 -6.38 -6.48 -13.14 1.48
Endoplasmic Reticulum 4.76 3.08 3.63 -7.17 2.42 2.16 -5.10 5.24 2.11 6.56 4.91 -2.64 4.96 -0.71 7.90
Endosome 4.46 2.73 -1.64 -2.58 -2.16 -1.03 -5.60 -1.63 -0.85 3.22 0.15 -4.63 -2.75 -2.72 -1.59
Golgi 2.71 4.77 -1.77 -2.06 -2.13 -1.60 -5.16 -1.69 1.28 0.47 -1.64 -1.42 -2.27 -1.09 -2.21
Lipid Particles 4.90 3.98 3.21 -6.62 -2.75 2.43 -10.10 -5.31 -6.22 4.82 4.34 -5.34 -5.45 -8.95 -0.35
Mitochondria 5.44 -3.12 -3.51 -8.50 -1.60 -2.09 -8.07 -10.10 -10.22 2.35 -1.11 -14.61 -11.64 -12.93 0.99
Mitotic Spindle 0.71 0.80 -2.87 -6.90 -7.24 -1.57 -1.53 -7.14 -5.99 -2.29 -0.98 -0.64 -9.57 -9.10 -8.87
None -9.16 -4.80 -10.02 -1.88 -7.50 -4.38 6.13 -8.01 -3.27 -12.43 -6.20 2.44 -9.76 -3.77 -12.52
Nuclear Periphery 5.49 -9.80 -12.40 -19.33 -1.77 -1.79 -11.13 -13.14 -13.01 6.32 0.84 -16.11 -19.32 -22.75 3.73
Nuclear Periphery Foci 2.18 2.00 1.98 -0.89 -0.04 6.45 -0.89 6.34 -0.44 5.27 6.48 3.12 5.72 -0.92 2.43
Nucleolus -0.85 -3.58 -6.39 -1.54 -4.34 -2.00 -4.83 -2.46 -0.86 -0.32 -2.33 -6.31 -3.76 -1.41 -1.91
Nucleus -4.35 -10.86 -0.93 6.86 19.05 3.21 -0.36 -1.96 -4.90 -0.36 -1.95 -18.19 -1.28 0.63 17.56
Peroxisomes 3.49 2.56 1.52 -2.45 -1.10 -2.53 -5.26 -1.38 1.00 0.86 -2.31 -3.93 -1.67 0.55 -0.61
Vacuole 5.95 0.74 -8.03 -21.15 -11.77 -2.23 -10.46 -17.47 -16.01 6.76 1.65 -11.33 -24.71 -26.02 -3.89
Vacuole Periphery -0.82 3.16 -1.30 0.28 -2.30 1.03 0.09 -1.04 -1.88 -1.28 -0.29 0.70 -1.31 -0.91 -2.34
Standard name
Human Ortholog
Description WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Wwm1

Wwm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wwm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available