Standard name
Human Ortholog
Description Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 – 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.07 0.06 0 – 0 0 0.05 0 0.09 0.16 0.19 0.09 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0.15 0 0.09 0.15 – 0.32 0.28 0.57 0.33 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.89 0.93 0.93 0.86 0.87 0.89 – 0.81 0.87 0.73 0.77 0.9 0.82 0.8 0.8 0.77 0.79 0.77 0.72 0.78 0.76 0.7 0.67
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 – 0 0 0.07 0.05 0 0 0 0.05 0 0.06 0.1 0.1 0.09 0.09 0.11 0.13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.05 0 0 0.06 – 0 0.05 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0.06 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 1 0 0 0 – 0 3 1 3 0 0 0 0 0 0 0 5 1 0 1 3
Bud Neck 0 0 2 0 0 1 1 – 1 2 1 0 1 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 1 0 8 – 4 9 11 12 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 1 15 4 3 10 10 6 – 5 6 14 11 17 35 53 16 23 11 0 1 0 4 4 4
Endoplasmic Reticulum 0 0 0 0 1 0 0 – 1 0 0 0 1 1 0 5 10 4 0 0 0 0 0 1
Endosome 0 5 1 0 4 0 0 – 0 1 0 1 0 0 0 4 8 2 1 5 1 0 3 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 2 1 0 1 0 0 0 1
Mitochondria 47 6 1 12 4 14 25 – 38 55 166 93 1 0 0 14 11 5 1 3 0 1 4 3
Nucleus 123 270 94 75 131 138 152 – 96 169 214 215 167 185 218 150 149 106 113 245 94 119 167 154
Nuclear Periphery 1 0 0 0 0 1 0 – 0 2 0 1 0 0 1 0 1 0 0 0 0 0 1 1
Nucleolus 12 13 2 1 3 3 2 – 2 6 20 15 1 4 3 9 7 8 15 34 11 14 26 28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 6 5 4 5 0 11 – 3 9 2 5 9 10 15 5 1 4 2 8 2 6 7 7
Vac/Vac Membrane 0 10 2 2 1 5 1 – 1 1 0 5 2 1 1 5 7 8 10 25 7 5 18 17
Unique Cell Count 139 304 101 81 153 158 170 119 195 293 281 185 225 273 188 194 135 147 339 121 156 239 231
Labelled Cell Count 186 325 111 98 160 172 206 151 263 429 361 200 236 292 211 219 150 147 339 121 156 239 231


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.1 5.9 4.9 6.4 5.2 5.0 – 4.7 4.5 4.2 4.5 6.3 6.2 6.0 7.9 8.3 8.2 5.7 5.9 6.2
Std Deviation (1e-4) 0.6 1.1 0.7 0.6 1.9 1.5 0.9 – 1.4 0.9 1.7 1.0 1.1 1.2 1.1 2.0 2.2 2.2 1.1 1.4 1.3
Intensity Change (Log2) – – – -0.27 0.11 -0.2 -0.25 – -0.34 -0.38 -0.5 -0.4 0.08 0.07 0.01 0.41 0.48 0.46 -0.06 0.0 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.9 0.8 0 – 0 0 0 0 1.6 3.0 3.7 1.5 2.2 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 0 2.6 3.7 – 0 0 0 0 0 0 0 2.4 0 0
Nucleus -0.1 -1.8 -1.5 -1.0 – -2.7 -1.7 -4.2 -3.6 -0.8 -2.6 -3.1 -3.0 -3.5 -3.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.5 – 0 0 0 0 0 0 0.2 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5247 -0.0417 -0.1036 -0.5705 -0.7978 -0.2347 0.262 0.4219 0.4973 0.0932 -0.1931 0.5062 0.6855 0.5059 0.4268 0.6898 0.0841 0.6579
Actin 0.0127 0.0001 0.0098 0 0.001 0.0007 0.0106 0.0001 0.0061 0.017 0.002 0.0002 0.0164 0 0.0244 0.0107 0 0.0036
Bud 0.0002 0 0.0007 0 0.0003 0 0.0002 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0006 0.0001 0 0.0004
Bud Neck 0.0006 0.0002 0.0002 0 0.0013 0.0014 0.002 0.0001 0.0002 0.0001 0.0008 0.002 0.0035 0.0002 0.0007 0.0004 0.0007 0.0013
Bud Periphery 0.0007 0 0.0013 0 0.0023 0 0.0006 0 0.0003 0.0003 0.0002 0.0009 0.0002 0.0001 0.001 0.0001 0.0001 0.0009
Bud Site 0.0016 0.0002 0.002 0 0.0008 0.0001 0.0007 0.0001 0.0008 0.0028 0.0004 0.0003 0.0043 0.0002 0.0018 0.0003 0.0001 0.0013
Cell Periphery 0.0002 0 0.0001 0 0.0008 0 0.0001 0 0 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0.0001
Cytoplasm 0.0085 0.0005 0.0014 0.0001 0.0002 0.0002 0.0021 0.0012 0.0011 0.0013 0.0028 0.0013 0.0027 0.0012 0.0005 0.0041 0.0001 0.0007
Cytoplasmic Foci 0.0068 0.0001 0.0037 0 0.0004 0.0017 0.0108 0.0003 0.0118 0.0073 0.0102 0.0073 0.0073 0 0.0039 0.0027 0 0.0032
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0008 0.0002 0.0009 0 0.0006 0 0.001 0.0001 0.0002 0.0015 0.0009 0.0002 0.0007 0.0001 0.0001 0.0006 0 0.0005
Endosome 0.0199 0.0001 0.0086 0 0.0147 0.0006 0.0094 0.0002 0.0073 0.011 0.0091 0.0083 0.0045 0.0001 0.0049 0.0057 0 0.0061
Golgi 0.0028 0 0.0024 0 0.0026 0.0003 0.0036 0 0.0035 0.0043 0.0101 0.0009 0.0031 0 0.0056 0.0067 0 0.0046
Lipid Particles 0.0199 0 0.0034 0 0.0062 0.0012 0.0118 0.0001 0.0236 0.0042 0.0573 0.0016 0.0084 0 0.0051 0.0186 0 0.0165
Mitochondria 0.013 0.0001 0.0152 0.0001 0.1513 0.0001 0.0162 0.0003 0.0164 0.002 0.0194 0.0024 0.0027 0.0002 0.0035 0.002 0.0004 0.022
None 0.0225 0.0163 0.0009 0 0.0003 0.0001 0.0242 0.0047 0.0049 0.0016 0.0016 0.0025 0.0176 0.0001 0.0002 0.0246 0 0.0044
Nuclear Periphery 0.0064 0.0056 0.0089 0.0004 0.0038 0.0008 0.023 0.0027 0.0021 0.0035 0.0111 0.0109 0.007 0.0037 0.002 0.0061 0.0007 0.006
Nucleolus 0.0217 0.0263 0.0164 0.0054 0.0664 0.0564 0.0142 0.0179 0.0167 0.0053 0.0363 0.0383 0.013 0.0227 0.0114 0.0086 0.046 0.0389
Nucleus 0.8001 0.9473 0.9085 0.9416 0.6849 0.9214 0.8185 0.9651 0.8588 0.9274 0.7414 0.9019 0.8808 0.9582 0.9139 0.8949 0.9481 0.8658
Peroxisomes 0.0053 0 0.0108 0 0.0009 0.0012 0.0283 0 0.0218 0.0042 0.025 0.0004 0.0055 0 0.0029 0.0027 0 0.0079
Punctate Nuclear 0.0495 0.0026 0.0016 0.0523 0.0013 0.0132 0.0191 0.0067 0.0226 0.0051 0.0674 0.0184 0.0195 0.013 0.0166 0.0105 0.0032 0.0138
Vacuole 0.0051 0.0002 0.0018 0 0.034 0.0005 0.0014 0.0001 0.0007 0.0003 0.0028 0.001 0.0015 0.0001 0.0004 0.0002 0.0002 0.001
Vacuole Periphery 0.0015 0.0001 0.0014 0 0.0257 0 0.002 0.0001 0.001 0.0003 0.0009 0.0007 0.0006 0 0.0002 0.0003 0.0001 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4007 9.4541 6.2359 8.1554 8.7604 9.3955 10.8867 11.4697 13.1903 10.0894
Translational Efficiency 0.7566 0.9515 1.3414 1.1082 0.7716 0.7313 0.7395 0.6522 0.5373 0.6973

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1294 1883 182 1279 1197 1642 110 14 2491 3525 292 1293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.37 809.89 1018.23 862.30 730.26 775.51 1038.94 337.19 664.34 793.88 1026.03 856.61
Standard Deviation 73.66 112.10 119.33 127.18 90.12 107.09 107.27 5.27 103.64 111.12 115.37 137.67
Intensity Change Log 2 — 0.424683 0.754949 0.515147 — 0.086735 0.508630 -1.114848 — 0.249488 0.625303 -0.152937

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000125 0.000642 0.002261 0.002276 0.000231 0.000476 0.002862 0.000216 0.000176 0.000565 0.002487 0.002254
Bud Neck 0.002519 0.013768 0.015464 0.021834 0.004768 0.011907 0.008902 0.000016 0.003599 0.012902 0.012992 0.021598
Bud Site 0.001450 0.001874 0.002708 0.018184 0.000938 0.002542 0.002616 0.000036 0.001204 0.002185 0.002674 0.017988
Cell Periphery 0.000214 0.000079 0.000082 0.000325 0.000085 0.000115 0.000113 0.000517 0.000152 0.000096 0.000093 0.000327
Cytoplasm 0.043800 0.007756 0.000686 0.060463 0.010285 0.018308 0.003163 0.000066 0.027695 0.012671 0.001619 0.059809
Cytoplasmic Foci 0.014095 0.002904 0.000077 0.009170 0.003283 0.003187 0.000229 0.000022 0.008899 0.003036 0.000134 0.009071
Eisosomes 0.000041 0.000031 0.000069 0.000039 0.000027 0.000036 0.000098 0.002848 0.000034 0.000033 0.000079 0.000069
Endoplasmic Reticulum 0.001639 0.004651 0.011876 0.005273 0.001830 0.004387 0.018438 0.000001 0.001730 0.004528 0.014348 0.005216
Endosome 0.000833 0.001414 0.000437 0.014462 0.000859 0.002132 0.000724 0.000000 0.000845 0.001748 0.000545 0.014305
Golgi 0.000440 0.001025 0.000206 0.007065 0.001091 0.000748 0.000213 0.000004 0.000753 0.000896 0.000209 0.006988
Lipid Particles 0.003386 0.001439 0.000414 0.001006 0.001689 0.002239 0.001118 0.000056 0.002570 0.001811 0.000679 0.000996
Mitochondria 0.003781 0.006267 0.001514 0.008409 0.002316 0.010715 0.001977 0.000013 0.003077 0.008339 0.001688 0.008318
Mitotic Spindle 0.001821 0.005913 0.000830 0.069073 0.002460 0.009937 0.002363 0.000297 0.002128 0.007787 0.001408 0.068328
None 0.038195 0.005435 0.001623 0.021288 0.007706 0.010734 0.002917 0.995356* 0.023544 0.007903 0.002110 0.031835
Nuclear Periphery 0.001140 0.002682 0.000535 0.003654 0.001038 0.002413 0.001377 0.000000 0.001091 0.002557 0.000852 0.003614
Nuclear Periphery Foci 0.000854 0.001393 0.000306 0.002351 0.000427 0.001103 0.000723 0.000001 0.000649 0.001258 0.000464 0.002326
Nucleolus 0.039877 0.047456 0.033550 0.013005 0.044008 0.053519 0.021160 0.000001 0.041862 0.050280 0.028883 0.012864
Nucleus 0.841367* 0.889760* 0.921153* 0.714825* 0.914356* 0.859324* 0.916641* 0.000007 0.876441* 0.875582* 0.919454* 0.707085*
Peroxisomes 0.002188 0.001599 0.001115 0.001539 0.001351 0.001529 0.001016 0.000542 0.001786 0.001566 0.001077 0.001528
Vacuole 0.001576 0.002796 0.004804 0.017727 0.000672 0.003103 0.013037 0.000000 0.001142 0.002939 0.007906 0.017535
Vacuole Periphery 0.000660 0.001116 0.000288 0.008033 0.000582 0.001546 0.000313 0.000000 0.000623 0.001317 0.000298 0.007946

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.65 -13.23 -8.89 -5.80 3.27 NAN NAN NAN NAN NAN -10.17 -12.44 -8.57 -6.39 4.94
Bud Neck -13.17 -9.46 -17.47 -8.43 -3.25 NAN NAN NAN NAN NAN -14.51 -10.35 -16.70 -9.47 -3.89
Bud Site -1.19 -3.56 -8.18 -8.03 -6.37 NAN NAN NAN NAN NAN -2.66 -5.64 -8.60 -7.66 -5.33
Cell Periphery 6.79 5.99 0.83 -7.06 -6.03 NAN NAN NAN NAN NAN 4.07 2.85 -2.42 -6.01 -5.02
Cytoplasm 11.61 14.67 0.50 -11.31 -14.48 NAN NAN NAN NAN NAN 7.42 15.26 -4.05 -9.41 -14.12
Cytoplasmic Foci 7.57 9.92 7.38 0.02 -4.39 NAN NAN NAN NAN NAN 6.61 10.54 6.04 0.37 -4.29
Eisosomes 4.05 -5.55 -3.71 -8.58 3.64 NAN NAN NAN NAN NAN 1.06 -9.48 -3.02 -3.16 0.50
Endoplasmic Reticulum -8.92 -8.52 -14.78 -7.02 3.70 NAN NAN NAN NAN NAN -9.70 -12.09 -16.65 -7.22 6.48
Endosome -2.01 1.94 -4.52 -3.83 -4.92 NAN NAN NAN NAN NAN -2.96 1.03 -4.49 -3.39 -4.68
Golgi -1.65 1.63 -2.56 -1.64 -2.91 NAN NAN NAN NAN NAN -0.39 1.61 -2.00 -1.87 -2.82
Lipid Particles 5.04 9.14 8.25 2.57 -2.54 NAN NAN NAN NAN NAN 2.31 7.93 7.95 4.18 -0.14
Mitochondria -2.29 2.88 -0.69 1.67 -3.90 NAN NAN NAN NAN NAN -5.62 2.31 -1.65 3.64 -3.48
Mitotic Spindle -3.22 -0.26 -11.76 -10.54 -11.81 NAN NAN NAN NAN NAN -4.97 -1.64 -11.72 -10.22 -11.23
None 11.43 12.92 8.37 -5.23 -8.56 NAN NAN NAN NAN NAN 9.22 13.24 0.28 -4.80 -6.78
Nuclear Periphery -4.86 3.22 -5.72 -1.82 -6.91 NAN NAN NAN NAN NAN -6.56 0.47 -5.85 -2.24 -6.00
Nuclear Periphery Foci -2.17 3.09 -2.04 -0.06 -3.88 NAN NAN NAN NAN NAN -3.83 0.54 -2.82 -0.51 -3.02
Nucleolus -2.34 0.17 9.83 12.95 4.77 NAN NAN NAN NAN NAN -3.66 1.63 12.52 16.83 5.45
Nucleus -6.74 -7.46 6.18 12.85 12.74 NAN NAN NAN NAN NAN 0.03 -3.25 11.86 12.27 13.12
Peroxisomes 1.35 2.40 3.39 3.31 2.97 NAN NAN NAN NAN NAN 0.83 2.40 3.80 4.51 3.18
Vacuole -1.88 -3.34 -10.50 -9.50 -7.17 NAN NAN NAN NAN NAN -4.06 -5.21 -10.75 -9.69 -4.59
Vacuole Periphery -1.37 1.54 -2.31 -1.70 -2.79 NAN NAN NAN NAN NAN -2.57 1.39 -2.36 -1.47 -2.75
Standard name
Human Ortholog
Description Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Swc3

Swc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available