Standard name
Human Ortholog
Description 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.09 0.12 0.12 0.18 0.17 0.19 0.25 0.13 0.12 0.1 0.15 0.1 0.19 0.21 – 0.17 0.14 0.08 0.09 0 0.09 0.06 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.56 0.06 0.31 0 0.28 0.25 0.2 0.71 0.62 0.62 0.56 0.05 0 0 – 0 0 0 0 0.05 0 0 0
Nucleus 0.65 0.6 0.76 0.75 0.79 0.68 0.63 0.59 0.58 0.57 0.65 0.56 0.9 0.77 0.74 – 0.67 0.65 0.69 0.6 0.6 0.62 0.57 0.36
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.05 0 0.05
Vac/Vac Membrane 0.18 0 0 0 0.05 0.05 0 0.05 0 0 0 0.05 0 0 0 – 0.15 0.18 0.16 0.17 0.16 0.13 0.23 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Bud 0 0 1 1 0 1 2 0 3 3 2 1 0 0 0 – 0 1 0 1 0 1 0 2
Bud Neck 0 0 1 0 1 0 2 3 1 1 0 0 0 0 1 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 2 1 1 7 10 4 11 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 11 11 6 10 29 28 54 70 31 34 15 31 4 27 37 – 13 18 8 12 2 6 7 28
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 1 0 1 3 – 2 9 1 0 0 0 0 0
Endosome 0 0 0 0 2 1 10 3 0 0 0 0 0 2 0 – 1 1 0 0 1 0 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 0 0
Mitochondria 29 69 3 26 3 47 71 55 171 175 98 118 2 4 1 – 3 4 1 6 5 1 4 6
Nucleus 70 75 39 64 130 114 178 166 139 161 102 118 37 108 128 – 52 85 77 89 58 47 66 65
Nuclear Periphery 1 0 0 0 1 0 1 0 0 5 0 1 0 0 0 – 0 1 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 0 2 0 3 2 0 1 0 – 0 2 0 0 1 0 1 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 4 1 2 5 1 4 0 5 0 5 1 – 1 1 1 4 5 3 1 9
Vac/Vac Membrane 19 5 1 2 8 8 12 15 5 4 3 10 1 4 7 – 12 23 18 25 15 9 26 58
Unique Cell Count 107 124 51 85 165 167 282 281 240 281 157 212 41 140 174 78 131 113 148 97 77 117 184
Labelled Cell Count 130 160 54 104 178 202 335 319 360 397 227 298 44 152 178 84 146 113 148 97 77 117 184


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.4 4.9 4.4 6.2 4.8 5.0 5.2 3.8 3.9 3.7 4.4 4.9 5.1 5.0 4.3 5.4 5.8 5.8 6.3 5.9
Std Deviation (1e-4) 0.7 0.7 1.1 0.8 2.6 2.1 2.1 2.6 1.6 1.3 1.1 3.3 0.8 1.1 0.9 0.9 1.5 1.1 1.5 2.3 1.4
Intensity Change (Log2) – – – -0.13 0.34 -0.03 0.04 0.1 -0.35 -0.32 -0.38 -0.16 0.02 0.08 0.05 -0.18 0.15 0.26 0.26 0.38 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.5 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5548 -0.6124 -0.5575 -0.35 -1.0135 -0.6625 3.8533 2.8651 2.7477 2.5664 2.3123 2.6543 0.3583 -0.0082 -0.11 0.4795 -0.9288 0.1504
Actin 0.0254 0.0004 0.0112 0.0025 0.0238 0.0028 0.0087 0.0002 0.0017 0.0076 0.0019 0.0005 0.0813 0.0003 0.0139 0.0005 0 0.002
Bud 0.0003 0.0001 0.0003 0.0002 0.0001 0.0003 0.0003 0.0001 0.0002 0.0004 0.0009 0 0.0005 0.0002 0.0003 0.0001 0 0.0001
Bud Neck 0.0024 0.01 0.0179 0.0018 0.001 0.0026 0.0114 0.0004 0.0043 0.0169 0.0017 0.0021 0.0033 0.0004 0.004 0.0003 0.0002 0.0034
Bud Periphery 0.0008 0.0001 0.0005 0.0001 0.0002 0.0005 0.0005 0.0002 0.0003 0.0005 0.0077 0.0001 0.0007 0.0002 0.0004 0.0001 0 0.0001
Bud Site 0.009 0.0006 0.0054 0.0026 0.0003 0.0005 0.0071 0.0005 0.0054 0.0082 0.0004 0.0001 0.0026 0.0011 0.0019 0.0005 0 0.0002
Cell Periphery 0.0019 0.0001 0.0002 0.0001 0 0 0.0002 0 0.0007 0.0002 0.0004 0 0.0003 0.0001 0.0002 0.0001 0 0
Cytoplasm 0.0331 0.0399 0.0515 0.021 0.0085 0.028 0.064 0.0285 0.0408 0.032 0.0614 0.035 0.1205 0.0784 0.0876 0.0357 0.02 0.021
Cytoplasmic Foci 0.0125 0.0027 0.0038 0.0121 0.0026 0.0097 0.0113 0.0008 0.0036 0.0044 0.0018 0.0064 0.0101 0.0048 0.0039 0.0108 0.0001 0.0012
Eisosomes 0.0014 0 0.0002 0.0001 0.0001 0 0.0007 0 0.0001 0.0001 0.0001 0 0.0004 0 0.0008 0 0 0
Endoplasmic Reticulum 0.0067 0.0036 0.0042 0.0028 0.002 0.0015 0.0072 0.0013 0.0024 0.001 0.0034 0.0017 0.022 0.0027 0.003 0.0009 0.0003 0.0035
Endosome 0.0259 0.0044 0.0084 0.0174 0.0136 0.0048 0.0141 0.0012 0.003 0.004 0.0058 0.0059 0.0259 0.0015 0.0038 0.0174 0.0005 0.0028
Golgi 0.0071 0.0001 0.0065 0.0025 0.0048 0.0007 0.0058 0 0.0005 0.0034 0.0011 0.0019 0.0096 0.0001 0.002 0.0032 0 0.0002
Lipid Particles 0.0129 0.0006 0.0037 0.0054 0.0064 0.003 0.0148 0.0002 0.0005 0.0011 0.0005 0.0057 0.0126 0.0002 0.0036 0.0406 0 0.0001
Mitochondria 0.0065 0.0007 0.0128 0.0009 0.002 0.0011 0.0126 0.0039 0.0008 0.0007 0.0126 0.0015 0.0036 0.0006 0.0119 0.004 0.0002 0.0004
None 0.0497 0.0553 0.084 0.0045 0.0033 0.0419 0.1736 0.0717 0.121 0.0766 0.1841 0.0544 0.1029 0.1482 0.0916 0.157 0.0018 0.0271
Nuclear Periphery 0.037 0.053 0.0504 0.0173 0.0317 0.0205 0.0457 0.0224 0.0244 0.0098 0.0261 0.0066 0.0441 0.0163 0.0124 0.0101 0.0053 0.0096
Nucleolus 0.0037 0.008 0.0074 0.0088 0.0109 0.0153 0.006 0.0069 0.0114 0.0053 0.0087 0.0076 0.0024 0.0042 0.0033 0.0036 0.0029 0.0076
Nucleus 0.7291 0.7912 0.6993 0.8231 0.8684 0.8284 0.5709 0.8393 0.7475 0.8127 0.6392 0.843 0.5131 0.715 0.7071 0.6889 0.9608 0.8903
Peroxisomes 0.0075 0.0002 0.0022 0.001 0.0018 0.0024 0.0203 0.0001 0.001 0.0011 0.0001 0.0011 0.0268 0.0001 0.0199 0.0128 0 0
Punctate Nuclear 0.023 0.0267 0.0259 0.0734 0.0168 0.0347 0.0203 0.0188 0.0287 0.0132 0.033 0.0254 0.0108 0.0224 0.0267 0.011 0.0072 0.0296
Vacuole 0.0023 0.0016 0.0026 0.0019 0.0008 0.0008 0.0026 0.0011 0.0013 0.0007 0.0045 0.0006 0.0046 0.0028 0.0012 0.0018 0.0005 0.0007
Vacuole Periphery 0.0018 0.0007 0.0017 0.0005 0.0008 0.0004 0.0019 0.0025 0.0004 0.0002 0.0044 0.0002 0.002 0.0004 0.0003 0.0006 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6153 5.0197 2.7936 10.468 5.4604 1.403 8.5648 12.8459 8.4936 9.9263
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
21 912 378 1781 2001 1761 522 1671 2022 2673 900 3452

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1081.01 772.51 921.03 828.85 682.35 802.50 870.11 810.50 686.49 792.27 891.50 819.97
Standard Deviation 238.32 94.01 101.64 112.03 81.79 94.41 98.77 118.04 94.04 95.34 103.10 115.35
Intensity Change Log 2 — -0.484754 -0.231060 -0.383197 — 0.233989 0.350686 0.248300 — -0.162966 0.022551 -0.105203

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002919 0.000520 0.000710 0.001571 0.000038 0.000138 0.000234 0.001463 0.000068 0.000268 0.000434 0.001518
Bud Neck 0.007350 0.009192 0.004442 0.015418 0.002148 0.007816 0.001483 0.011030 0.002202 0.008286 0.002725 0.013294
Bud Site 0.030156 0.007381 0.000733 0.022391 0.000527 0.003853 0.000611 0.014555 0.000835 0.005057 0.000662 0.018597
Cell Periphery 0.000245 0.000237 0.000056 0.000172 0.000090 0.000233 0.000131 0.000330 0.000091 0.000234 0.000100 0.000248
Cytoplasm 0.022939 0.156868* 0.008018 0.141802* 0.206769* 0.141586* 0.032948 0.135464* 0.204860* 0.146800* 0.022477 0.138734*
Cytoplasmic Foci 0.064375 0.052032 0.000183 0.004055 0.016308 0.031516 0.002917 0.004834 0.016807 0.038516 0.001769 0.004432
Eisosomes 0.000172 0.000064 0.000031 0.000033 0.000017 0.000043 0.000046 0.000033 0.000019 0.000050 0.000039 0.000033
Endoplasmic Reticulum 0.011919 0.003933 0.008016 0.006066 0.000688 0.000983 0.003155 0.005578 0.000805 0.001990 0.005197 0.005830
Endosome 0.080854 0.004309 0.000380 0.005084 0.000593 0.002737 0.000488 0.002558 0.001427 0.003273 0.000443 0.003861
Golgi 0.018446 0.001807 0.000059 0.002570 0.000106 0.002231 0.000039 0.001172 0.000297 0.002086 0.000047 0.001893
Lipid Particles 0.026643 0.006285 0.000371 0.001020 0.000933 0.003729 0.000954 0.000863 0.001200 0.004601 0.000709 0.000944
Mitochondria 0.005675 0.007272 0.000623 0.007677 0.000794 0.005139 0.000330 0.003260 0.000845 0.005867 0.000453 0.005539
Mitotic Spindle 0.050379 0.007343 0.000768 0.034877 0.000128 0.004361 0.000318 0.007362 0.000650 0.005379 0.000507 0.021558
None 0.003500 0.009695 0.001968 0.006466 0.016373 0.009115 0.007874 0.012892 0.016239 0.009313 0.005393 0.009577
Nuclear Periphery 0.010308 0.001557 0.000853 0.003665 0.000517 0.001015 0.000729 0.002200 0.000619 0.001200 0.000781 0.002956
Nuclear Periphery Foci 0.087621 0.001548 0.000864 0.001255 0.000290 0.000289 0.000248 0.001084 0.001197 0.000718 0.000506 0.001172
Nucleolus 0.049489 0.004817 0.003842 0.001402 0.002932 0.004444 0.002957 0.002158 0.003416 0.004571 0.003329 0.001768
Nucleus 0.430191* 0.702886* 0.963042* 0.708322* 0.746727* 0.769197* 0.938952* 0.769660* 0.743440* 0.746572* 0.949070* 0.738013*
Peroxisomes 0.022802 0.008092 0.000195 0.002796 0.000370 0.004290 0.000184 0.001188 0.000603 0.005587 0.000188 0.002018
Vacuole 0.060032 0.013247 0.004761 0.031820 0.003384 0.006441 0.005338 0.018279 0.003972 0.008763 0.005096 0.025265
Vacuole Periphery 0.013982 0.000913 0.000087 0.001540 0.000267 0.000845 0.000065 0.004037 0.000409 0.000868 0.000074 0.002749

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.45 1.91 1.99 -3.00 0.50 -4.30 -9.81 -3.13 -2.87 -2.33 -5.96 -11.62 -5.11 -4.11 -2.27
Bud Neck -0.84 0.10 -4.38 -4.43 -6.69 -5.35 -0.45 -10.31 -3.59 -10.46 -7.17 -4.35 -14.93 -6.00 -12.22
Bud Site 0.99 1.19 0.77 -2.73 -7.52 -4.36 -1.62 -7.75 -5.19 -7.36 -5.21 -1.04 -10.65 -6.14 -10.56
Cell Periphery 0.25 3.28 2.32 3.55 -4.50 -4.34 -2.07 -5.61 -1.06 -3.78 -5.92 -1.44 -6.30 1.06 -4.45
Cytoplasm -12.58 1.93 -10.05 5.15 -22.97 8.89 27.55 11.66 2.88 -15.74 8.62 31.57 13.72 5.58 -25.46
Cytoplasmic Foci 0.58 3.14 3.01 12.88 -7.21 -5.94 9.99 9.03 13.34 -2.06 -9.12 11.64 10.35 18.69 -4.00
Eisosomes 2.54 3.01 3.02 4.36 -0.03 -8.83 -8.20 -11.05 0.37 2.78 -12.06 -9.03 -13.98 2.77 2.14
Endoplasmic Reticulum 3.08 0.65 1.43 -9.57 2.44 -3.94 -11.80 -19.67 -18.52 -4.98 -10.20 -16.87 -29.69 -22.54 -1.68
Endosome 2.64 2.77 2.70 1.95 -4.75 -5.48 -0.84 -5.89 0.67 -5.23 -3.87 1.89 -2.81 1.63 -6.91
Golgi 3.03 3.33 3.17 1.52 -2.95 -3.99 2.84 -2.67 2.35 -2.92 -4.61 3.00 -2.86 2.70 -4.18
Lipid Particles 1.46 1.81 1.78 7.87 -3.44 -5.57 -1.00 -0.39 5.49 0.77 -7.59 1.54 1.15 9.02 -0.95
Mitochondria -0.59 1.73 0.46 2.22 -4.90 -5.76 2.55 -5.44 2.42 -6.60 -7.75 1.70 -6.61 2.89 -7.53
Mitotic Spindle 1.53 1.74 0.96 -4.91 -8.14 -3.86 -1.43 -6.96 -2.88 -6.21 -4.54 -0.80 -10.55 -6.29 -10.12
None -4.06 0.73 -1.97 3.47 -6.25 4.50 5.08 3.48 -0.87 -2.00 4.60 7.26 5.59 1.42 -3.15
Nuclear Periphery 1.01 1.05 0.52 -12.63 -16.06 -4.79 -6.73 -14.36 -10.02 -9.03 -4.78 -5.59 -21.52 -17.36 -18.17
Nuclear Periphery Foci 1.92 1.85 1.95 1.47 1.62 -0.22 -0.93 -4.14 -4.06 -3.40 0.19 -1.22 -1.14 -2.01 0.75
Nucleolus 2.57 2.37 2.73 3.50 2.67 -2.42 -0.26 0.01 2.42 0.27 -2.18 -1.51 1.32 3.73 2.46
Nucleus -3.07 -5.74 -3.47 -2.79 22.57 -2.22 -21.96 -2.63 -0.36 18.21 -0.07 -25.43 -1.05 -1.02 28.87
Peroxisomes 1.91 2.77 2.68 5.71 -1.90 -5.45 2.67 -1.45 4.19 -1.96 -7.91 2.44 -1.47 6.78 -2.84
Vacuole 3.16 3.58 -0.08 -13.39 -15.88 -3.48 -4.12 -13.00 -11.44 -5.75 -5.47 -4.55 -20.96 -18.29 -15.03
Vacuole Periphery 2.40 2.55 2.44 1.03 -3.49 -2.94 1.94 -3.04 -2.32 -3.28 -2.78 2.71 -3.08 -1.99 -3.92
Standard name
Human Ortholog
Description 5'- and 3'-flap DNA binding protein; recruits Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; complexes with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
Localization
Cell Percentages nucleus (39%), cytoplasm (6%), mixed (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Saw1

Saw1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Saw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available