Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 5 | 1 | 1 | 3 | 2 | 3 | 6 | 3 | 4 | 3 | 6 | 0 | 1 | 4 | 5 | 5 | 1 | 12 | 13 | 15 | 11 | 30 | 29 |
Bud | 1 | 2 | 5 | 0 | 1 | 3 | 3 | 4 | 15 | 5 | 5 | 5 | 6 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 10 | 7 | 4 | 7 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 26 | 52 | 38 | 16 | 30 | 15 | 15 | 19 | 18 | 13 | 14 | 19 | 28 | 59 | 75 | 66 | 60 | 53 | 24 | 17 | 30 | 35 | 80 | 41 |
Cytoplasm | 147 | 243 | 204 | 177 | 237 | 230 | 243 | 303 | 173 | 154 | 134 | 112 | 237 | 275 | 346 | 225 | 171 | 150 | 95 | 197 | 138 | 37 | 63 | 88 |
Endoplasmic Reticulum | 2 | 4 | 0 | 1 | 1 | 5 | 1 | 1 | 3 | 0 | 1 | 2 | 1 | 2 | 2 | 16 | 6 | 9 | 14 | 7 | 3 | 6 | 15 | 14 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 1 | 0 | 1 | 3 | 11 | 22 | 26 | 54 | 52 | 71 | 57 | 0 | 0 | 1 | 6 | 3 | 1 | 6 | 7 | 22 | 5 | 16 | 13 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 11 | 4 | 8 | 0 | 12 | 3 | 2 | 4 | 2 |
Unique Cell Count | 153 | 256 | 206 | 182 | 248 | 248 | 265 | 321 | 203 | 195 | 171 | 161 | 239 | 277 | 357 | 261 | 196 | 171 | 167 | 272 | 227 | 104 | 227 | 214 |
Labelled Cell Count | 182 | 308 | 249 | 197 | 282 | 270 | 292 | 365 | 270 | 233 | 234 | 205 | 272 | 339 | 436 | 338 | 252 | 227 | 167 | 272 | 227 | 104 | 227 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.1 | 5.4 | 5.2 | 6.0 | 4.5 | 4.9 | 5.0 | 4.1 | 4.0 | 3.9 | 4.3 | 5.3 | 5.5 | 5.8 | 8.9 | 8.8 | 9.0 | 5.3 | 5.5 | 6.0 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.8 | 1.2 | 1.7 | 0.9 | 1.3 | 1.1 | 1.0 | 0.9 | 1.3 | 0.9 | 0.9 | 1.0 | 1.0 | 1.5 | 1.4 | 1.5 | 1.2 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.17 | -0.26 | -0.13 | -0.11 | -0.38 | -0.43 | -0.44 | -0.31 | -0.01 | 0.04 | 0.12 | 0.74 | 0.71 | 0.74 | -0.0 | 0.04 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -1.9 | 0 | -4.4 | -4.5 | 0 | 0 | 0 | 0 | -2.0 | 0.8 | 0.7 | 1.8 | 2.8 | 2.8 |
Cytoplasm | -1.3 | -2.2 | -3.3 | -3.6 | -2.7 | -5.2 | -6.5 | -6.6 | -8.1 | 0.1 | 0.3 | -1.6 | -5.0 | -4.7 | -4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.2 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.308 | 1.1629 | 0.8148 | 0.6397 | 0.9649 | 1.0273 | 0.7627 | 1.2838 | 1.0232 | 0.7847 | 1.0012 | 0.8079 | 0.8191 | 1.1538 | 0.6648 | 0.5795 | 0.3675 | 0.7512 |
Actin | 0.0465 | 0.0218 | 0.0258 | 0.0204 | 0.0233 | 0.0332 | 0.0632 | 0.0365 | 0.0229 | 0.0743 | 0.0378 | 0.0441 | 0.0561 | 0.025 | 0.0208 | 0.0014 | 0.0834 | 0.0182 |
Bud | 0.0005 | 0.0004 | 0.0012 | 0.0014 | 0.0124 | 0.0004 | 0.0015 | 0.0006 | 0.001 | 0.0009 | 0.0006 | 0.0011 | 0.0009 | 0.0007 | 0.0012 | 0.001 | 0.0014 | 0.0011 |
Bud Neck | 0.0021 | 0.0035 | 0.0029 | 0.0087 | 0.008 | 0.0137 | 0.0072 | 0.0023 | 0.0033 | 0.0013 | 0.0023 | 0.0111 | 0.0043 | 0.002 | 0.0029 | 0.002 | 0.008 | 0.0071 |
Bud Periphery | 0.0014 | 0.0011 | 0.0065 | 0.0047 | 0.043 | 0.003 | 0.0035 | 0.0013 | 0.002 | 0.0029 | 0.002 | 0.005 | 0.0037 | 0.0015 | 0.0035 | 0.0016 | 0.0043 | 0.0069 |
Bud Site | 0.0041 | 0.0373 | 0.0148 | 0.0036 | 0.0094 | 0.001 | 0.0142 | 0.052 | 0.0197 | 0.0018 | 0.0009 | 0.0011 | 0.008 | 0.0431 | 0.0113 | 0.0049 | 0.011 | 0.0011 |
Cell Periphery | 0.0313 | 0.0257 | 0.0196 | 0.0149 | 0.0041 | 0.0209 | 0.0309 | 0.019 | 0.0098 | 0.0031 | 0.0022 | 0.0239 | 0.0232 | 0.022 | 0.0151 | 0.0017 | 0.013 | 0.0207 |
Cytoplasm | 0.5263 | 0.6275 | 0.6568 | 0.6917 | 0.6686 | 0.6992 | 0.6174 | 0.747 | 0.7994 | 0.805 | 0.7814 | 0.7993 | 0.6649 | 0.6804 | 0.7146 | 0.746 | 0.8017 | 0.8071 |
Cytoplasmic Foci | 0.0197 | 0.0059 | 0.0102 | 0.008 | 0.0093 | 0.0092 | 0.0428 | 0.0053 | 0.0144 | 0.0081 | 0.0536 | 0.0103 | 0.0198 | 0.0052 | 0.0053 | 0.0118 | 0.0215 | 0.0066 |
Eisosomes | 0.002 | 0.002 | 0.0015 | 0.0006 | 0.0006 | 0.0013 | 0.0036 | 0.001 | 0.0003 | 0.0006 | 0.0005 | 0.0007 | 0.0017 | 0.0009 | 0.0009 | 0.0006 | 0.0011 | 0.0006 |
Endoplasmic Reticulum | 0.0144 | 0.0053 | 0.0051 | 0.011 | 0.003 | 0.0069 | 0.0076 | 0.0019 | 0.0025 | 0.0058 | 0.0029 | 0.0048 | 0.0046 | 0.0021 | 0.004 | 0.0034 | 0.0024 | 0.0034 |
Endosome | 0.023 | 0.0025 | 0.0144 | 0.0177 | 0.0273 | 0.0027 | 0.0289 | 0.0008 | 0.0072 | 0.0104 | 0.0378 | 0.0024 | 0.0153 | 0.0004 | 0.0012 | 0.0033 | 0.0026 | 0.0021 |
Golgi | 0.006 | 0.0049 | 0.0058 | 0.0147 | 0.0311 | 0.0021 | 0.0144 | 0.002 | 0.0051 | 0.0152 | 0.0097 | 0.0042 | 0.0057 | 0.0011 | 0.0021 | 0.0006 | 0.0052 | 0.0021 |
Lipid Particles | 0.0167 | 0.0008 | 0.0036 | 0.0031 | 0.0036 | 0.002 | 0.0289 | 0.0004 | 0.0045 | 0.0132 | 0.0073 | 0.0018 | 0.0078 | 0.0006 | 0.0046 | 0.0004 | 0.0007 | 0.0027 |
Mitochondria | 0.0058 | 0.0005 | 0.0063 | 0.1008 | 0.0159 | 0.0088 | 0.0042 | 0.0001 | 0.0008 | 0.002 | 0.0009 | 0.0065 | 0.0029 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0023 |
None | 0.2586 | 0.2586 | 0.2133 | 0.0894 | 0.1286 | 0.1897 | 0.0959 | 0.1282 | 0.0968 | 0.0505 | 0.0258 | 0.0801 | 0.1557 | 0.2133 | 0.2 | 0.2159 | 0.0409 | 0.1149 |
Nuclear Periphery | 0.0089 | 0.0002 | 0.0016 | 0.0011 | 0.001 | 0.0025 | 0.0091 | 0.0001 | 0.0006 | 0.0015 | 0.0117 | 0.0002 | 0.0023 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0006 |
Nucleolus | 0.0008 | 0.0002 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0168 | 0.0004 | 0.0008 | 0.0009 | 0.0014 | 0.0007 | 0.003 | 0.0003 | 0.0008 | 0.0005 | 0.0044 | 0.0003 | 0.002 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0004 |
Peroxisomes | 0.0081 | 0.0002 | 0.002 | 0.0015 | 0.0015 | 0.0005 | 0.0168 | 0.0002 | 0.006 | 0.001 | 0.0069 | 0.002 | 0.0108 | 0.0002 | 0.0088 | 0.0001 | 0.0014 | 0.0004 |
Punctate Nuclear | 0.0027 | 0.0002 | 0.0039 | 0.0004 | 0.0008 | 0.0004 | 0.0025 | 0.0001 | 0.0008 | 0.0006 | 0.0079 | 0.0002 | 0.0068 | 0.0001 | 0.0007 | 0.0005 | 0.0002 | 0.0003 |
Vacuole | 0.0035 | 0.001 | 0.0029 | 0.0037 | 0.005 | 0.001 | 0.0028 | 0.0006 | 0.0019 | 0.001 | 0.0019 | 0.0008 | 0.0019 | 0.0007 | 0.0011 | 0.0028 | 0.0007 | 0.0012 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0005 | 0.0015 | 0.0014 | 0.0005 | 0.001 | 0 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5581 | 11.6654 | 7.0532 | 11.2896 | 7.833 | 6.7626 | 12.2317 | 13.7102 | 12.3894 | 13.0275 |
Translational Efficiency | 0.8702 | 0.4785 | 0.6818 | 0.5965 | 0.7599 | 0.7284 | 0.6653 | 0.5164 | 0.4824 | 0.4704 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
535 | 1123 | 1592 | 1227 | 1302 | 1448 | 114 | 1800 | 1837 | 2571 | 1706 | 3027 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 668.40 | 777.49 | 948.64 | 971.05 | 729.44 | 823.13 | 1046.54 | 944.31 | 711.66 | 803.19 | 955.18 | 955.15 |
Standard Deviation | 74.94 | 132.35 | 109.37 | 115.94 | 84.04 | 104.72 | 108.77 | 128.80 | 86.08 | 119.75 | 112.03 | 124.44 |
Intensity Change Log 2 | — | 0.218112 | 0.505149 | 0.538834 | — | 0.174331 | 0.520766 | 0.372471 | — | 0.195432 | 0.513320 | 0.454416 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002721 | 0.012948 | 0.016254 | 0.164679* | 0.004126 | 0.011738 | 0.018360 | 0.039272 | 0.003717 | 0.012266 | 0.016395 | 0.090106 |
Bud Neck | 0.028896 | 0.044959 | 0.008586 | 0.011952 | 0.035428 | 0.056508 | 0.005322 | 0.011493 | 0.033525 | 0.051463 | 0.008368 | 0.011679 |
Bud Site | 0.008616 | 0.043498 | 0.048395 | 0.114575* | 0.013328 | 0.042884 | 0.024476 | 0.055638 | 0.011956 | 0.043152 | 0.046797 | 0.079528 |
Cell Periphery | 0.001845 | 0.005555 | 0.002211 | 0.015150 | 0.003999 | 0.005062 | 0.004885 | 0.002974 | 0.003372 | 0.005277 | 0.002389 | 0.007909 |
Cytoplasm | 0.419783* | 0.440386* | 0.577937* | 0.376478* | 0.531008* | 0.544000* | 0.306367* | 0.503331* | 0.498615* | 0.498742* | 0.559790* | 0.451911* |
Cytoplasmic Foci | 0.299240* | 0.226283* | 0.088134 | 0.041219 | 0.223133* | 0.164045 | 0.187815* | 0.084732 | 0.245298* | 0.191230* | 0.094795 | 0.067094 |
Eisosomes | 0.009284 | 0.014173 | 0.001110 | 0.019775 | 0.013954 | 0.016911 | 0.003069 | 0.006273 | 0.012594 | 0.015715 | 0.001241 | 0.011746 |
Endoplasmic Reticulum | 0.002397 | 0.003964 | 0.011351 | 0.006230 | 0.003007 | 0.001914 | 0.008195 | 0.002900 | 0.002829 | 0.002809 | 0.011140 | 0.004250 |
Endosome | 0.035516 | 0.033828 | 0.004144 | 0.003018 | 0.017261 | 0.016010 | 0.024152 | 0.010667 | 0.022577 | 0.023793 | 0.005481 | 0.007567 |
Golgi | 0.017990 | 0.036256 | 0.001348 | 0.002526 | 0.026610 | 0.023649 | 0.001534 | 0.003318 | 0.024099 | 0.029155 | 0.001361 | 0.002997 |
Lipid Particles | 0.041973 | 0.026409 | 0.004514 | 0.003664 | 0.020718 | 0.009006 | 0.016392 | 0.009408 | 0.026908 | 0.016607 | 0.005308 | 0.007080 |
Mitochondria | 0.004790 | 0.007640 | 0.000248 | 0.000531 | 0.006205 | 0.006004 | 0.000141 | 0.000716 | 0.005793 | 0.006718 | 0.000241 | 0.000641 |
Mitotic Spindle | 0.000070 | 0.001353 | 0.002140 | 0.004271 | 0.000188 | 0.003489 | 0.000196 | 0.010344 | 0.000153 | 0.002556 | 0.002010 | 0.007882 |
None | 0.002059 | 0.001532 | 0.001086 | 0.000974 | 0.001489 | 0.000930 | 0.000193 | 0.000717 | 0.001655 | 0.001193 | 0.001026 | 0.000821 |
Nuclear Periphery | 0.000622 | 0.000324 | 0.000266 | 0.000090 | 0.000101 | 0.000054 | 0.000433 | 0.000221 | 0.000253 | 0.000172 | 0.000277 | 0.000168 |
Nuclear Periphery Foci | 0.000523 | 0.001022 | 0.004297 | 0.001359 | 0.000351 | 0.000409 | 0.009588 | 0.001775 | 0.000401 | 0.000677 | 0.004651 | 0.001606 |
Nucleolus | 0.000480 | 0.000407 | 0.000087 | 0.000039 | 0.000250 | 0.000502 | 0.000308 | 0.000299 | 0.000317 | 0.000460 | 0.000102 | 0.000194 |
Nucleus | 0.034587 | 0.020592 | 0.115729 | 0.030795 | 0.040450 | 0.023072 | 0.056929 | 0.038817 | 0.038742 | 0.021989 | 0.111800 | 0.035565 |
Peroxisomes | 0.016394 | 0.018492 | 0.001680 | 0.005462 | 0.011884 | 0.014032 | 0.006681 | 0.006795 | 0.013198 | 0.015980 | 0.002014 | 0.006255 |
Vacuole | 0.071080 | 0.058284 | 0.110351 | 0.196036 | 0.045348 | 0.058026 | 0.324703* | 0.209669* | 0.052842 | 0.058139 | 0.124674 | 0.204143* |
Vacuole Periphery | 0.001136 | 0.002096 | 0.000133 | 0.001176 | 0.001163 | 0.001757 | 0.000260 | 0.000641 | 0.001155 | 0.001905 | 0.000141 | 0.000858 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.29 | -6.87 | -19.10 | -17.36 | -16.85 | -6.90 | -2.09 | -10.77 | -7.48 | -2.21 | -9.81 | -6.83 | -21.07 | -18.06 | -15.87 |
Bud Neck | -4.61 | 8.45 | 7.62 | 13.17 | -2.31 | -6.81 | 15.89 | 13.20 | 17.89 | -5.74 | -7.73 | 16.43 | 15.31 | 22.15 | -3.04 |
Bud Site | -15.07 | -14.51 | -19.98 | -11.34 | -10.64 | -12.73 | -2.86 | -13.65 | -2.07 | -6.18 | -18.79 | -13.77 | -23.40 | -9.40 | -7.82 |
Cell Periphery | -5.37 | -0.59 | -8.00 | -4.70 | -8.03 | -1.97 | -0.35 | 3.43 | 5.09 | 1.68 | -4.33 | 3.72 | -5.05 | -1.68 | -7.41 |
Cytoplasm | -1.67 | -11.39 | 3.45 | 6.00 | 17.58 | -1.51 | 11.28 | 4.49 | 5.92 | -9.35 | -0.00 | -4.60 | 7.36 | 7.73 | 11.22 |
Cytoplasmic Foci | 7.33 | 24.58 | 30.12 | 31.12 | 13.20 | 9.64 | 3.39 | 26.71 | 17.00 | 7.65 | 10.12 | 31.29 | 39.84 | 32.63 | 8.22 |
Eisosomes | -2.22 | 4.80 | -2.75 | -0.68 | -9.56 | -1.74 | 7.47 | 6.70 | 8.27 | -2.04 | -2.34 | 12.02 | 2.68 | 5.32 | -11.10 |
Endoplasmic Reticulum | -2.19 | -9.98 | -4.37 | -1.93 | 6.28 | 2.13 | -2.57 | 0.76 | -1.29 | 2.78 | 0.04 | -10.37 | -2.09 | -2.11 | 9.16 |
Endosome | 0.45 | 11.24 | 11.68 | 13.28 | 3.24 | 1.03 | -1.89 | 6.09 | 5.73 | 4.34 | -0.80 | 15.41 | 13.22 | 13.84 | -2.02 |
Golgi | -7.75 | 10.75 | 9.70 | 18.42 | -1.36 | 1.80 | 20.41 | 18.04 | 16.73 | -2.19 | -3.61 | 23.41 | 20.68 | 24.67 | -2.91 |
Lipid Particles | 3.50 | 10.23 | 10.57 | 9.37 | 3.30 | 8.68 | 1.58 | 8.03 | 0.64 | 2.77 | 5.72 | 15.34 | 13.76 | 8.04 | -1.41 |
Mitochondria | -1.95 | 4.39 | 4.17 | 6.87 | -1.23 | 0.13 | 5.11 | 4.66 | 4.83 | -2.39 | -0.78 | 6.22 | 5.83 | 7.94 | -2.34 |
Mitotic Spindle | -1.60 | -2.72 | -3.33 | -1.70 | -0.91 | -2.86 | -0.03 | -5.38 | -2.76 | -5.38 | -3.25 | -2.62 | -6.24 | -3.29 | -3.48 |
None | 0.54 | 1.28 | 1.36 | 1.02 | 0.45 | 1.53 | 4.64 | 2.55 | 0.92 | -3.26 | 1.11 | 2.12 | 2.50 | 1.36 | 0.94 |
Nuclear Periphery | 1.09 | 1.24 | 2.05 | 2.15 | 9.43 | 4.73 | -5.94 | -4.63 | -6.51 | 3.64 | 0.92 | -0.90 | 0.99 | -0.11 | 6.31 |
Nuclear Periphery Foci | -1.92 | -16.16 | -8.62 | -1.54 | 11.78 | -0.82 | -8.31 | -7.18 | -6.08 | 6.98 | -2.29 | -18.46 | -9.93 | -5.38 | 12.03 |
Nucleolus | 0.82 | 6.19 | 6.92 | 6.43 | 7.14 | -1.23 | -0.66 | -0.22 | 0.93 | 0.13 | -1.22 | 6.86 | 1.35 | 1.82 | -0.90 |
Nucleus | 6.63 | -22.50 | 1.10 | -6.52 | 24.28 | 9.35 | -1.78 | 0.71 | -8.53 | 1.96 | 11.79 | -22.62 | 1.62 | -10.79 | 23.67 |
Peroxisomes | -1.00 | 8.87 | 6.75 | 9.86 | -7.18 | -1.72 | 2.05 | 5.52 | 6.66 | 0.12 | -2.57 | 14.53 | 8.78 | 11.36 | -6.88 |
Vacuole | 2.67 | -10.94 | -22.05 | -25.08 | -14.36 | -4.52 | -13.68 | -33.98 | -31.13 | 4.87 | -2.24 | -20.41 | -41.01 | -39.93 | -16.14 |
Vacuole Periphery | -4.02 | 9.07 | 0.50 | 2.45 | -1.81 | -1.12 | 7.11 | 2.42 | 2.15 | -1.26 | -2.35 | 11.05 | 1.93 | 3.21 | -2.49 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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