Standard name
Human Ortholog
Description Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.08 0.05 0.08 0.1 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.98 0.91 0.89 0.79 0.77 0.73 0.69 0.66 0.57 0.97 0.97 0.98 1.0 0.95 0.98 0.96 0.96 0.91 0.92 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0.08 0.07 0.15 0.13 0.15 0.14 0.14 0.12 0 0 0 0.06 0.08 0.05 0 0 0 0 0.08 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.14 0.21 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 6 23 28 19 30 25 32 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 2 0 0 0 1 1 2 0 6 0 0 0 0 0 0 0 1 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 6 10 13 20 40 25 25 40 31 40 4 1 2 1 1 0 0 0 0 0 0 0
Cytoplasm 190 206 395 306 304 289 402 372 237 273 174 169 194 146 181 48 69 40 185 200 395 101 126 152
Endoplasmic Reticulum 3 4 16 3 27 23 75 61 48 57 36 37 6 3 2 3 6 2 2 0 15 3 11 15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Golgi 1 0 0 0 0 0 2 4 3 2 5 5 0 0 1 0 1 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 2 6 12 21 38 56 55 80 0 0 2 0 1 1 0 0 2 1 0 0
Nucleus 0 0 2 1 0 2 1 2 0 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 0 1 1 1 6 0 3 1 4 0 0 0 1 3 0 1 3 8 0 0 1
Unique Cell Count 194 208 409 311 334 326 507 483 323 394 265 297 200 150 184 48 73 41 193 209 433 110 145 180
Labelled Cell Count 199 213 421 323 349 347 556 520 371 466 329 376 204 150 189 53 81 43 193 209 433 110 145 180


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 71.3 56.5 38.3 33.6 30.9 26.4 23.4 21.7 19.9 18.9 17.5 16.6 59.8 53.3 46.7 76.5 75.2 77.8 59.8 65.2 65.6
Std Deviation (1e-4) 16.2 13.9 8.3 8.0 6.8 6.1 5.1 4.9 4.7 4.5 4.3 4.2 12.9 13.3 12.2 24.6 21.2 27.0 19.6 20.6 20.1
Intensity Change (Log2) – – – -0.19 -0.31 -0.53 -0.71 -0.82 -0.94 -1.02 -1.13 -1.2 0.64 0.48 0.29 1.0 0.98 1.02 0.64 0.77 0.78

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700050100WT3HU80HU120HU160050100WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3050100WT1AF100AF140AF180050100
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 4.4 4.9 5.0 5.7 6.3 6.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 2.1 3.4 4.4 3.0 4.2 5.3 5.7 6.4 0 0 0 0 0 0
Cytoplasm 1.5 -3.2 -4.2 -7.7 -8.4 -9.1 -10.3 -10.8 -13.0 0.3 0.4 1.2 1.3 -0.9 0.3
Endoplasmic Reticulum -2.4 2.4 1.9 5.5 4.6 5.2 5.2 4.6 4.3 -0.6 -1.1 -1.9 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 4.3 7.1 7.9 9.6 11.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 48.8142 68.6527 64.5215 66.3663 54.8954 62.6437 50.6407 69.5537 65.3652 63.492 64.2093 62.9937 58.4381 57.2452 63.8025 56.1274 59.549 62.8418
Actin 0.0051 0.0002 0.0003 0.0001 0.0011 0.0003 0.0025 0.001 0.0002 0.0004 0.0023 0.0003 0.0044 0.0006 0.0011 0.0007 0.0008 0.0027
Bud 0.0011 0.0005 0.0006 0.001 0.001 0.0007 0.0009 0.0004 0.0005 0.0007 0.0002 0.0005 0.0007 0.0008 0.0018 0.0021 0.0004 0.0035
Bud Neck 0.0011 0.001 0.0008 0.0007 0.0015 0.002 0.0015 0.0005 0.0005 0.0008 0.0005 0.0015 0.0007 0.0004 0.0007 0.0005 0.001 0.0025
Bud Periphery 0.0019 0.0005 0.0006 0.0006 0.0026 0.0008 0.0017 0.0005 0.0004 0.0008 0.0002 0.0009 0.0008 0.0003 0.0007 0.0012 0.0002 0.0028
Bud Site 0.0007 0.0005 0.0004 0.0001 0.0005 0.0002 0.0012 0.0003 0.0003 0.0002 0.0001 0.0001 0.0006 0.0004 0.0004 0.0016 0.0001 0.0004
Cell Periphery 0.0179 0.0168 0.0147 0.0118 0.0167 0.0058 0.0222 0.0121 0.0103 0.0138 0.0058 0.0052 0.0063 0.0009 0.0011 0.0035 0.0003 0.0022
Cytoplasm 0.5 0.8136 0.7228 0.6802 0.6798 0.7057 0.6657 0.8862 0.8506 0.7122 0.7833 0.8376 0.7779 0.9556 0.9133 0.7391 0.9178 0.8133
Cytoplasmic Foci 0.0076 0.0007 0.0038 0.0011 0.0095 0.0058 0.005 0.0008 0.0029 0.0019 0.0015 0.0028 0.0047 0.0026 0.0047 0.0034 0.0063 0.0067
Eisosomes 0.0002 0 0 0 0.0008 0.0001 0.0006 0 0 0 0 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.1399 0.0376 0.0616 0.009 0.0597 0.133 0.1049 0.0295 0.0404 0.027 0.0135 0.0732 0.0763 0.0169 0.0186 0.0052 0.0175 0.0526
Endosome 0.013 0.002 0.0052 0.0073 0.0093 0.0118 0.0076 0.0022 0.0043 0.0066 0.013 0.0109 0.0125 0.0039 0.01 0.0165 0.0139 0.0287
Golgi 0.0015 0.0002 0.0003 0.0001 0.0009 0.001 0.0015 0.0011 0.0005 0.0005 0.0019 0.0013 0.0014 0.0011 0.001 0.0004 0.0013 0.0055
Lipid Particles 0.0042 0.0009 0.0023 0.0004 0.0055 0.0019 0.0054 0.0015 0.0018 0.0022 0.0026 0.0005 0.0019 0.0001 0.0013 0.0007 0.0001 0.0002
Mitochondria 0.0078 0.0003 0.0004 0.0003 0.009 0.0047 0.0089 0.0012 0.0013 0.0003 0.0016 0.0017 0.0025 0.0008 0.0006 0.005 0.0005 0.0079
None 0.0045 0.0001 0.0011 0.0001 0.0055 0.0019 0.0048 0.0003 0.0015 0.0003 0.0003 0.0003 0.0035 0.0002 0.0003 0.0033 0.0003 0.0005
Nuclear Periphery 0.0459 0.009 0.0114 0.0206 0.0143 0.0142 0.0195 0.0032 0.0044 0.0067 0.0181 0.0075 0.0121 0.0015 0.004 0.0071 0.0053 0.0086
Nucleolus 0.0024 0.0001 0.0002 0.0002 0.0024 0.0011 0.0069 0.0001 0.0009 0.0003 0.0008 0.0001 0.0002 0 0 0.0033 0 0.0005
Nucleus 0.0139 0.0031 0.003 0.0058 0.0058 0.0039 0.0093 0.0015 0.0023 0.003 0.0036 0.0018 0.0034 0.0012 0.0016 0.0061 0.0017 0.0043
Peroxisomes 0.0003 0 0.0001 0 0.0006 0.0002 0.0014 0 0.0001 0 0.0001 0 0.0012 0 0.0001 0.0002 0.0001 0.0001
Punctate Nuclear 0.0048 0 0.001 0 0.0009 0.0002 0.0018 0 0.0003 0.0001 0.0005 0 0.005 0 0.0001 0.0002 0 0.0001
Vacuole 0.1754 0.1047 0.1488 0.206 0.1353 0.0844 0.1105 0.0542 0.0706 0.2064 0.1223 0.0446 0.0703 0.0108 0.0316 0.1429 0.0238 0.0401
Vacuole Periphery 0.0507 0.0081 0.0205 0.0544 0.0374 0.0204 0.016 0.0031 0.0059 0.0158 0.0277 0.0092 0.0137 0.0017 0.0071 0.0568 0.0084 0.0168

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 264.7584 309.4513 299.6817 225.3595 255.3136 273.0413 232.2615 213.8614 229.2232 203.7688
Translational Efficiency 2.026 1.9246 1.5184 2.3683 1.7554 1.5116 1.5482 1.6834 1.3272 1.5556

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1439 722 237 792 2153 1520 99 1756 3592 2242 336 2548

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3504.00 4103.36 6160.64 4872.94 3489.79 3632.60 5873.79 4690.53 3495.48 3784.20 6076.12 4747.23
Standard Deviation 719.37 840.91 928.86 1428.42 638.41 821.08 1151.48 1053.49 672.05 856.25 1008.14 1185.84
Intensity Change Log 2 — 0.227803 0.814077 0.475790 — 0.057862 0.751151 0.426611 — 0.145506 0.783021 0.451460

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000109 0.000155 0.000150 0.000313 0.000039 0.000081 0.000247 0.000128 0.000067 0.000105 0.000178 0.000186
Bud Neck 0.000333 0.001046 0.003629 0.004909 0.000155 0.000389 0.010620 0.001070 0.000226 0.000601 0.005689 0.002263
Bud Site 0.004186 0.005857 0.002751 0.017197 0.002518 0.003082 0.003392 0.003618 0.003186 0.003975 0.002940 0.007839
Cell Periphery 0.012334 0.013669 0.024306 0.006056 0.004839 0.007250 0.030187 0.014622 0.007841 0.009317 0.026039 0.011960
Cytoplasm 0.674234 0.644563 0.373822 0.399796 0.774987 0.826711 0.164533 0.627273 0.734624 0.768053 0.312157 0.556566
Cytoplasmic Foci 0.012818 0.015364 0.023084 0.011824 0.019608 0.008058 0.026003 0.006384 0.016888 0.010411 0.023944 0.008074
Eisosomes 0.000002 0.000003 0.000006 0.000008 0.000001 0.000001 0.000012 0.000002 0.000001 0.000002 0.000008 0.000004
Endoplasmic Reticulum 0.001500 0.001217 0.000438 0.000416 0.002035 0.000680 0.000867 0.000684 0.001821 0.000853 0.000564 0.000601
Endosome 0.001004 0.001760 0.001169 0.005750 0.001046 0.000738 0.002147 0.000872 0.001029 0.001067 0.001457 0.002388
Golgi 0.096033 0.114014 0.105766 0.025459 0.063282 0.051183 0.070566 0.037882 0.076403 0.071417 0.095395 0.034021
Lipid Particles 0.006731 0.019499 0.019119 0.014470 0.006353 0.008061 0.034082 0.015605 0.006504 0.011744 0.023528 0.015252
Mitochondria 0.000314 0.000167 0.000190 0.001120 0.000328 0.000149 0.000174 0.000252 0.000322 0.000155 0.000185 0.000522
Mitotic Spindle 0.001473 0.002474 0.003698 0.006632 0.000907 0.000772 0.004386 0.000677 0.001133 0.001320 0.003901 0.002528
None 0.002366 0.001532 0.001837 0.002051 0.000986 0.002694 0.000688 0.000919 0.001539 0.002320 0.001498 0.001271
Nuclear Periphery 0.000451 0.000880 0.004645 0.000661 0.000225 0.000274 0.005036 0.000532 0.000316 0.000469 0.004760 0.000572
Nuclear Periphery Foci 0.010392 0.019191 0.032826 0.008791 0.008350 0.011309 0.034262 0.010422 0.009168 0.013847 0.033249 0.009915
Nucleolus 0.000090 0.000111 0.000165 0.001125 0.000031 0.000049 0.000213 0.000097 0.000055 0.000069 0.000179 0.000417
Nucleus 0.005997 0.006987 0.015381 0.026305 0.001446 0.004508 0.035848 0.007097 0.003269 0.005306 0.021411 0.013068
Peroxisomes 0.000019 0.000020 0.000012 0.000401 0.000029 0.000018 0.000021 0.000035 0.000025 0.000018 0.000015 0.000149
Vacuole 0.040628 0.076198 0.247348 0.409911 0.034773 0.038964 0.470087 0.214148 0.037119 0.050954 0.312976 0.274998
Vacuole Periphery 0.128985 0.075292 0.139659 0.056806 0.078063 0.035031 0.106630 0.057680 0.098463 0.047996 0.129927 0.057408

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.20 -2.35 -3.92 -2.88 -2.63 -2.87 -5.87 -2.64 -1.70 2.17 -3.09 -6.61 -3.92 -2.77 -0.06
Bud Neck -5.90 -7.34 -9.30 -8.25 -3.68 -4.41 -7.16 -10.10 -7.28 6.52 -6.97 -9.58 -11.59 -9.91 4.87
Bud Site -2.19 2.41 -6.38 -5.47 -7.16 -1.21 -2.80 -3.75 -1.69 0.40 -1.94 -0.09 -6.93 -5.50 -6.77
Cell Periphery -1.07 -5.25 7.77 7.64 8.67 -3.96 -6.84 -11.76 -7.62 3.78 -2.45 -9.46 -5.66 -3.01 7.24
Cytoplasm 1.96 13.01 18.42 13.57 -1.76 -5.59 24.73 15.15 19.42 -17.77 -3.91 23.06 20.87 22.47 -12.80
Cytoplasmic Foci -2.21 -4.70 -0.66 1.14 4.00 10.63 -1.93 12.58 1.87 3.78 7.72 -3.79 9.72 2.20 6.73
Eisosomes -3.28 -6.09 -3.51 -2.83 -0.38 -1.62 -7.61 -5.07 -2.59 6.61 -2.70 -9.69 -4.97 -3.67 5.03
Endoplasmic Reticulum 1.20 5.08 5.61 6.12 1.04 6.45 4.77 6.00 0.10 0.13 6.10 8.31 7.59 2.41 -0.49
Endosome -5.67 -1.66 -3.75 -2.90 -3.48 3.65 -3.73 2.02 -1.45 4.26 -0.62 -3.67 -3.26 -3.08 -1.53
Golgi -2.62 -1.12 18.83 16.44 14.30 3.44 0.55 9.47 4.87 3.05 1.55 -2.76 18.92 14.25 12.34
Lipid Particles -6.67 -7.75 -4.35 2.21 2.74 -1.78 -5.53 -9.62 -6.60 3.74 -5.77 -9.12 -10.30 -3.13 4.59
Mitochondria 2.05 1.49 -1.76 -2.23 -2.11 3.88 1.29 0.05 -0.90 -0.36 4.08 2.60 -1.07 -2.09 -1.69
Mitotic Spindle -1.81 -3.81 -2.59 -2.01 -1.22 1.19 -2.94 2.46 0.57 3.09 -0.88 -5.84 -2.16 -1.86 2.55
None 2.79 1.74 0.98 -1.00 -0.24 -2.60 1.80 0.32 2.62 -0.76 -1.70 0.14 1.54 2.29 1.47
Nuclear Periphery -1.93 -8.11 -1.95 0.66 7.59 -1.84 -6.57 -9.94 -7.17 6.10 -1.99 -10.53 -5.17 -1.82 9.85
Nuclear Periphery Foci -5.60 -7.39 1.40 6.47 7.88 -3.05 -4.67 -3.19 0.88 4.24 -5.53 -9.09 -1.15 4.73 8.82
Nucleolus -0.60 -4.41 -3.14 -3.05 -2.87 -3.81 -4.46 -14.97 -8.13 3.19 -1.15 -7.52 -3.58 -3.40 -1.97
Nucleus -1.18 -4.99 -10.18 -9.69 -6.06 -8.73 -4.30 -11.45 -5.91 3.72 -4.97 -6.08 -12.89 -10.88 2.26
Peroxisomes -0.24 1.38 -2.24 -2.23 -2.28 2.65 0.69 -0.96 -2.74 -1.40 2.01 2.97 -2.34 -2.50 -2.54
Vacuole -7.03 -14.79 -31.73 -26.76 -7.98 -1.59 -15.43 -29.88 -27.99 8.42 -5.52 -19.96 -41.37 -37.03 2.52
Vacuole Periphery 7.59 -0.73 14.37 5.52 10.13 12.79 -0.75 6.47 -7.44 2.78 14.87 -3.08 14.60 -1.63 9.63
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Fun12

Fun12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fun12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available