Standard name | |
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Human Ortholog | |
Description | Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 1 | 2 | 8 | 22 | 13 | 7 | 7 | 14 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 5 | 8 | 6 | 11 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 1 | 6 | 15 | 19 | 11 | 18 | 36 | 21 | 19 | 15 | 29 | 9 | 6 | 16 | 33 | 8 | 12 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 259 | 239 | 325 | 266 | 351 | 265 | 304 | 359 | 255 | 276 | 205 | 248 | 244 | 274 | 292 | 405 | 184 | 222 | 244 | 238 | 322 | 134 | 258 | 210 |
Endoplasmic Reticulum | 3 | 7 | 11 | 9 | 14 | 13 | 23 | 58 | 32 | 25 | 25 | 20 | 3 | 5 | 4 | 11 | 5 | 9 | 5 | 3 | 8 | 5 | 7 | 7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 4 | 0 | 0 | 1 | 0 | 9 | 48 | 44 | 64 | 54 | 72 | 45 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 2 | 2 | 0 |
Nucleus | 0 | 0 | 5 | 2 | 4 | 1 | 8 | 4 | 3 | 2 | 3 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 3 | 6 | 11 | 3 | 15 | 26 | 6 | 23 | 5 | 24 | 1 | 1 | 1 | 6 | 0 | 1 | 1 | 1 | 13 | 4 | 4 | 1 |
Unique Cell Count | 266 | 245 | 337 | 282 | 382 | 294 | 382 | 492 | 346 | 369 | 288 | 337 | 248 | 286 | 303 | 414 | 187 | 228 | 259 | 247 | 357 | 150 | 279 | 223 |
Labelled Cell Count | 273 | 248 | 351 | 300 | 401 | 308 | 426 | 552 | 400 | 417 | 339 | 393 | 257 | 289 | 315 | 457 | 199 | 247 | 259 | 247 | 357 | 150 | 279 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.6 | 28.5 | 19.8 | 20.2 | 16.2 | 13.2 | 11.5 | 11.6 | 10.2 | 9.9 | 9.1 | 9.8 | 22.7 | 22.6 | 21.4 | 36.1 | 36.7 | 36.0 | 23.5 | 23.6 | 25.1 |
Std Deviation (1e-4) | 3.9 | 4.8 | 3.7 | 3.2 | 2.6 | 2.3 | 2.2 | 2.1 | 2.0 | 1.9 | 2.0 | 1.9 | 4.9 | 4.1 | 4.0 | 8.7 | 9.9 | 9.3 | 5.9 | 5.0 | 4.7 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.29 | -0.58 | -0.79 | -0.77 | -0.96 | -1.0 | -1.12 | -1.02 | 0.2 | 0.19 | 0.11 | 0.87 | 0.89 | 0.86 | 0.25 | 0.26 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.9 | 3.6 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | 2.3 | 1.5 | 2.2 | 3.6 | 2.9 | 2.4 | 2.4 | 4.0 | 1.4 | 0.3 | 2.4 | 3.8 | 0 | 2.3 |
Cytoplasm | -1.3 | -2.6 | -3.2 | -6.8 | -8.7 | -8.3 | -8.1 | -8.8 | -8.3 | 1.4 | -0.4 | 0 | 1.1 | 1.3 | 0.6 |
Endoplasmic Reticulum | -0.1 | 0.3 | 0.8 | 1.7 | 4.4 | 3.2 | 2.1 | 2.9 | 1.7 | -1.6 | -1.2 | -1.6 | -0.5 | -0.4 | 0.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 6.7 | 5.6 | 8.3 | 7.3 | 9.8 | 6.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.9 | 0 | 2.6 | 3.4 | 0 | 3.8 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0131 | 0.0002 | 0.0014 | 0.0023 | 0.0057 | 0.0007 | 0.0095 | 0.0003 | 0.0029 | 0.015 | 0.0002 | 0.0011 | 0.0095 | 0.0011 | 0.0152 | 0.0001 | 0 | 0.0002 |
Bud | 0.0005 | 0.0001 | 0.0004 | 0.0026 | 0.0018 | 0.0003 | 0.0005 | 0.0005 | 0.0016 | 0.0011 | 0.0002 | 0.0005 | 0.0034 | 0.001 | 0.0004 | 0.0003 | 0 | 0.0003 |
Bud Neck | 0.001 | 0.0001 | 0.0004 | 0.0005 | 0.0006 | 0.001 | 0.0004 | 0.0002 | 0.0004 | 0.0045 | 0.0004 | 0.0008 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0009 |
Bud Periphery | 0.0004 | 0 | 0.0002 | 0.0048 | 0.0043 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0055 | 0.0001 | 0.0003 | 0.0011 | 0 | 0 |
Bud Site | 0.0015 | 0.0002 | 0.0021 | 0.0006 | 0.0017 | 0.0001 | 0.0015 | 0.0007 | 0.0057 | 0.0064 | 0.0001 | 0.0001 | 0.0024 | 0.001 | 0.0005 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0007 | 0 | 0 |
Cytoplasm | 0.8185 | 0.9875 | 0.9228 | 0.8199 | 0.8185 | 0.8885 | 0.8021 | 0.9766 | 0.9027 | 0.8143 | 0.8475 | 0.9185 | 0.5402 | 0.9887 | 0.9437 | 0.7267 | 0.9943 | 0.9752 |
Cytoplasmic Foci | 0.019 | 0.0021 | 0.0085 | 0.0101 | 0.0235 | 0.0103 | 0.0225 | 0.0042 | 0.0115 | 0.0264 | 0.0154 | 0.0068 | 0.0033 | 0.0026 | 0.0026 | 0.0036 | 0.0026 | 0.0036 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0474 | 0.004 | 0.0194 | 0.0112 | 0.0114 | 0.0469 | 0.0465 | 0.0057 | 0.021 | 0.0045 | 0.002 | 0.0364 | 0.1198 | 0.0011 | 0.004 | 0.0011 | 0.0002 | 0.0029 |
Endosome | 0.0396 | 0.0023 | 0.0283 | 0.0598 | 0.0585 | 0.0334 | 0.058 | 0.0071 | 0.0148 | 0.0417 | 0.108 | 0.0169 | 0.0529 | 0.0017 | 0.0051 | 0.0198 | 0.0015 | 0.0093 |
Golgi | 0.0078 | 0.0004 | 0.003 | 0.0049 | 0.0119 | 0.0043 | 0.0078 | 0.0006 | 0.0049 | 0.0066 | 0.0103 | 0.0037 | 0.0081 | 0.0004 | 0.0054 | 0.0007 | 0 | 0.0005 |
Lipid Particles | 0.0054 | 0 | 0.0005 | 0.0002 | 0.0119 | 0.0004 | 0.0071 | 0.0001 | 0.0016 | 0.0105 | 0.0005 | 0 | 0.0011 | 0 | 0.0002 | 0.0005 | 0 | 0 |
Mitochondria | 0.0103 | 0.0003 | 0.0012 | 0.0403 | 0.0139 | 0.0036 | 0.0043 | 0.0001 | 0.011 | 0.0012 | 0.0023 | 0.008 | 0.0234 | 0.0001 | 0.0182 | 0.0043 | 0 | 0.0002 |
None | 0.0063 | 0.0003 | 0.0005 | 0.001 | 0.0013 | 0.001 | 0.0019 | 0.0002 | 0.0011 | 0.0013 | 0.0002 | 0.0005 | 0.003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0074 | 0.0004 | 0.0023 | 0.0042 | 0.0052 | 0.0016 | 0.0069 | 0.0005 | 0.0012 | 0.0135 | 0.0006 | 0.0013 | 0.1482 | 0.0003 | 0.0006 | 0.008 | 0.0001 | 0.0012 |
Nucleolus | 0.0005 | 0 | 0 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 | 0.0019 | 0.0007 | 0 | 0 | 0.0004 | 0 | 0 | 0.0007 | 0 | 0 |
Nucleus | 0.0023 | 0.0005 | 0.0012 | 0.0022 | 0.0021 | 0.0011 | 0.0018 | 0.0006 | 0.0029 | 0.0028 | 0.0006 | 0.001 | 0.0131 | 0.0004 | 0.0005 | 0.0022 | 0.0002 | 0.0015 |
Peroxisomes | 0.0027 | 0 | 0.0002 | 0.0001 | 0.0035 | 0.0001 | 0.0108 | 0 | 0.0092 | 0.0108 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0057 | 0 | 0.0002 | 0.0002 | 0.0108 | 0.0002 | 0.005 | 0 | 0.0006 | 0.014 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0083 | 0.0013 | 0.0059 | 0.0245 | 0.0084 | 0.0043 | 0.0101 | 0.0019 | 0.0033 | 0.0179 | 0.0081 | 0.0026 | 0.0262 | 0.0007 | 0.0017 | 0.073 | 0.0005 | 0.0028 |
Vacuole Periphery | 0.0018 | 0.0002 | 0.0014 | 0.0102 | 0.0037 | 0.0018 | 0.0023 | 0.0003 | 0.0009 | 0.0061 | 0.0028 | 0.0012 | 0.0375 | 0.0002 | 0.0005 | 0.1567 | 0.0001 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.9979 | 99.5262 | 104.8446 | 68.3201 | 96.0186 | 76.5001 | 73.2269 | 62.7645 | 65.2086 | 68.4924 |
Translational Efficiency | 2.239 | 1.884 | 1.494 | 2.1903 | 1.6409 | 1.5721 | 1.7786 | 1.7079 | 1.4374 | 1.6674 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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