Standard name
Human Ortholog
Description Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 1.0 0.99 1.0 1.0 1.0 1.0 0.98 0.99 0.99 0.98 0.96 0.99 0.99 0.99 0.97 0.99 1.0 0.95 0.98 0.96 0.94 0.96 0.95
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 0 0 1 0 4 3 1 2 6 2 1 5 1 1 2 3 0 0 0 0 0 0
Cytoplasm – 143 98 76 145 161 235 229 141 184 159 141 97 195 207 183 178 107 35 136 95 72 158 193
Endoplasmic Reticulum – 0 1 0 0 0 0 4 1 1 3 4 0 0 1 9 19 8 0 0 0 0 0 3
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 1 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Unique Cell Count 143 99 76 145 161 236 233 142 185 162 147 98 197 209 188 180 107 37 139 99 77 165 203
Labelled Cell Count 144 99 76 146 161 239 236 143 187 168 147 98 201 209 199 201 119 37 139 99 77 165 203


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 322.8 301.7 336.1 354.8 301.6 287.8 263.0 260.1 277.1 269.6 259.7 411.1 409.2 380.4 281.6 332.7 386.4 321.1 323.3 320.9
Std Deviation (1e-4) – 68.5 64.3 69.1 62.6 57.8 63.3 61.3 45.2 68.2 67.8 60.7 74.8 90.5 92.1 100.2 104.7 108.4 89.1 78.7 91.5
Intensity Change (Log2) – – – 0.16 0.23 -0.0 -0.07 -0.2 -0.21 -0.12 -0.16 -0.22 0.45 0.44 0.33 -0.1 0.14 0.36 0.09 0.1 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.2 1.3 0.6 -0.5 0.3 0.5 -0.5 -1.4 0 0 0 -0.9 -0.1 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0019 0.0001 0.0005 0.0002 0.0003 0.0072 0.0093 0.0002 0.0067 0.0001 0.0012 0.0006 – – – – – –
Bud 0.0005 0.0002 0.0006 0.0001 0.0158 0.0141 0.0022 0.0002 0.002 0.0001 0.0005 0.0005 – – – – – –
Bud Neck 0.0001 0.0001 0.0002 0.0009 0.0027 0.0028 0.0006 0.0001 0.0006 0.0001 0.0002 0.0003 – – – – – –
Bud Periphery 0.0002 0 0.0004 0 0.0033 0.0129 0.0008 0.0001 0.001 0 0.0003 0.0005 – – – – – –
Bud Site 0.0001 0 0.0005 0 0.0019 0.0006 0.0005 0.0002 0.0018 0 0.001 0.0001 – – – – – –
Cell Periphery 0.0002 0 0.0038 0 0.0035 0.0006 0.0007 0.0001 0.0033 0 0.0023 0.0005 – – – – – –
Cytoplasm 0.7431 0.9941 0.6665 0.9913 0.2255 0.498 0.794 0.963 0.829 0.9959 0.7485 0.8517 – – – – – –
Cytoplasmic Foci 0.0003 0.0001 0.0079 0.0001 0.0769 0.0014 0.0065 0.0002 0.0006 0 0.0055 0.0003 – – – – – –
Eisosomes 0 0 0.0012 0 0.0002 0 0.0002 0.0001 0.0021 0 0.0039 0 – – – – – –
Endoplasmic Reticulum 0.0003 0 0.0001 0.0001 0.0005 0.0037 0.0015 0 0.0002 0 0.0001 0.0001 – – – – – –
Endosome 0.0005 0 0.0006 0 0.0014 0.0095 0.0114 0 0.0005 0 0.0002 0.0003 – – – – – –
Golgi 0 0 0.0003 0 0.0002 0.0004 0.0018 0 0.0002 0 0.0002 0.0001 – – – – – –
Lipid Particles 0 0 0.0069 0 0.0014 0.0001 0.0055 0 0.0007 0 0.0052 0.0001 – – – – – –
Mitochondria 0.0134 0.0003 0.1412 0.0002 0.1531 0.0128 0.0117 0.0302 0.0589 0.0001 0.1294 0.1023 – – – – – –
None 0.0029 0.0002 0.0089 0.0001 0.0947 0.0028 0.0121 0.0002 0.002 0.0001 0.0032 0.0005 – – – – – –
Nuclear Periphery 0.074 0.0005 0.0014 0.0013 0.0019 0.1331 0.0502 0.0002 0.0097 0.0002 0.0005 0.0013 – – – – – –
Nucleolus 0.0037 0 0.0991 0 0.1535 0.0023 0.0046 0.0013 0.0134 0 0.0759 0.0045 – – – – – –
Nucleus 0.1468 0.0042 0.0122 0.0056 0.2005 0.1967 0.063 0.0032 0.0473 0.0034 0.0093 0.0105 – – – – – –
Peroxisomes 0.0001 0 0.0038 0 0.0044 0.0012 0.0018 0.0004 0.0024 0 0.0036 0.0004 – – – – – –
Punctate Nuclear 0.0002 0 0.0002 0 0.0025 0.0015 0.004 0 0.0002 0 0.0003 0.0001 – – – – – –
Vacuole 0.0016 0 0.0133 0.0001 0.0328 0.0419 0.0025 0.0001 0.0066 0 0.0027 0.0036 – – – – – –
Vacuole Periphery 0.0099 0 0.0305 0.0001 0.023 0.0565 0.0152 0.0003 0.0108 0 0.0059 0.022 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5559.235 5704.3125 6612.2392 5958.9495 6301.1657 6058.3944 6199.2673 5803.0844 6765.1497 5909.4939
Translational Efficiency 1.505 1.54 1.4475 1.473 1.4786 1.4128 1.5977 1.739 1.5462 1.762

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1753 178 1247 1259 234 517 1353 1339 1987 695 2600 2598

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 20085.15 24762.52 22797.33 33396.30 22308.88 23054.79 24641.03 31165.58 20347.03 23492.17 23756.76 32246.59
Standard Deviation 4271.81 5109.41 4271.48 8721.32 4283.79 5407.69 5256.37 8317.45 4332.92 5384.72 4896.65 8588.22
Intensity Change Log 2 — 0.302029 0.182736 0.733559 — 0.047448 0.143444 0.482335 — 0.173672 0.162193 0.606821

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000113 0.000064 0.000293 0.000109 0.000116 0.000060 0.000083 0.000025 0.000115 0.000062 0.000185
Bud Neck 0.000104 0.001825 0.001100 0.001210 0.001854 0.001441 0.000664 0.001203 0.000310 0.001539 0.000873 0.001206
Bud Site 0.000641 0.001951 0.001812 0.006106 0.001973 0.002429 0.002205 0.003281 0.000798 0.002307 0.002016 0.004650
Cell Periphery 0.001273 0.017277 0.012837 0.023397 0.021045 0.011564 0.005472 0.004836 0.003601 0.013027 0.009004 0.013831
Cytoplasm 0.940222* 0.789125* 0.828909* 0.708427* 0.777278* 0.786635* 0.860781* 0.779284* 0.921033* 0.787273* 0.845495* 0.744946*
Cytoplasmic Foci 0.001072 0.017075 0.004857 0.002944 0.015095 0.016361 0.003554 0.002852 0.002723 0.016544 0.004179 0.002896
Eisosomes 0.000000 0.000005 0.000002 0.000011 0.000005 0.000003 0.000001 0.000002 0.000001 0.000003 0.000002 0.000007
Endoplasmic Reticulum 0.000016 0.000018 0.000077 0.000137 0.000030 0.000028 0.000035 0.000039 0.000018 0.000025 0.000055 0.000087
Endosome 0.000016 0.000068 0.000091 0.000081 0.000090 0.000093 0.000071 0.000098 0.000025 0.000086 0.000081 0.000090
Golgi 0.001637 0.002467 0.004735 0.000579 0.004562 0.003422 0.001789 0.000678 0.001981 0.003178 0.003202 0.000630
Lipid Particles 0.000311 0.007522 0.007709 0.008862 0.008490 0.006267 0.002821 0.002745 0.001275 0.006589 0.005166 0.005709
Mitochondria 0.000063 0.000185 0.000082 0.000480 0.000384 0.000350 0.000103 0.000155 0.000100 0.000308 0.000093 0.000312
Mitotic Spindle 0.000062 0.000922 0.000268 0.000176 0.000479 0.000479 0.000048 0.000133 0.000111 0.000593 0.000153 0.000153
None 0.014368 0.006515 0.003033 0.006711 0.017396 0.008664 0.003270 0.006943 0.014725 0.008114 0.003156 0.006831
Nuclear Periphery 0.000049 0.000722 0.000565 0.000168 0.000798 0.000606 0.000182 0.000145 0.000137 0.000636 0.000366 0.000156
Nuclear Periphery Foci 0.000771 0.002541 0.003096 0.001255 0.003459 0.002929 0.001103 0.000894 0.001087 0.002830 0.002059 0.001069
Nucleolus 0.000029 0.000076 0.000059 0.000225 0.000160 0.000129 0.000096 0.000271 0.000044 0.000116 0.000078 0.000248
Nucleus 0.032700 0.103704 0.051576 0.049752 0.089187 0.117506 0.049595 0.077791 0.039352 0.113971 0.050545 0.064203
Peroxisomes 0.000007 0.000013 0.000008 0.000040 0.000015 0.000012 0.000018 0.000034 0.000008 0.000013 0.000013 0.000037
Vacuole 0.004559 0.046573 0.072789 0.187564 0.053015 0.039107 0.065277 0.117108 0.010266 0.041019 0.068880 0.151251
Vacuole Periphery 0.002086 0.001301 0.006330 0.001582 0.004575 0.001858 0.002856 0.001428 0.002379 0.001716 0.004522 0.001503

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.86 -12.96 -4.59 -1.88 -3.23 -0.38 1.34 0.83 1.84 -1.32 -6.26 -7.30 -5.70 -1.04 -3.46
Bud Neck -4.29 -5.79 -12.88 1.72 0.13 0.32 1.60 -0.66 -1.43 -4.59 -5.90 -6.29 -12.30 0.21 -3.31
Bud Site -3.57 -3.05 -6.47 -4.55 -3.93 -0.73 -0.72 -2.60 -1.35 -2.06 -2.86 -4.36 -7.92 -3.15 -4.32
Cell Periphery -6.82 -11.84 -12.41 0.31 -2.66 2.78 4.90 5.43 5.80 2.49 -7.87 -7.86 -8.57 2.21 -1.53
Cytoplasm 11.26 19.79 31.13 1.05 9.02 0.00 -4.26 1.31 1.97 11.74 18.30 19.65 35.11 2.70 14.58
Cytoplasmic Foci -6.50 -9.53 -5.76 5.54 3.32 -0.96 4.22 4.21 7.35 0.04 -10.13 -5.38 -2.99 9.27 2.40
Eisosomes -4.75 -8.74 -3.16 -0.43 -2.28 1.58 2.63 2.14 1.98 -2.86 -7.04 -5.23 -3.57 -0.77 -2.57
Endoplasmic Reticulum -1.10 -2.72 -2.55 -2.42 -0.48 0.57 -0.52 0.12 -0.78 0.89 -2.06 -3.49 -3.00 -2.34 -0.29
Endosome -3.50 -8.27 -6.97 0.02 2.06 0.15 1.00 -0.48 -0.64 -2.41 -3.80 -9.02 -8.46 -0.14 -0.55
Golgi -2.89 -8.34 4.72 7.16 13.48 1.49 4.92 7.34 4.53 7.64 -2.52 -4.94 6.40 5.78 15.28
Lipid Particles -5.78 -12.11 -10.58 0.52 0.43 1.21 3.21 3.09 3.44 -0.36 -7.47 -10.19 -9.25 1.97 0.16
Mitochondria -1.62 -3.03 -2.64 -0.93 -2.34 -0.09 1.40 1.40 2.25 -0.05 -2.69 -0.48 -2.35 1.14 -2.24
Mitotic Spindle -1.36 -3.87 -1.47 1.18 1.34 0.25 2.44 1.87 2.02 -1.95 -2.50 -1.50 -1.14 2.18 0.06
None 6.97 11.32 6.41 0.35 -2.21 2.41 3.90 3.28 2.65 -1.35 6.80 11.41 7.98 2.68 -2.43
Nuclear Periphery -6.83 -9.10 -13.08 5.77 6.99 0.86 4.35 4.49 5.17 0.43 -8.22 -7.89 -3.77 7.41 6.51
Nuclear Periphery Foci -5.13 -9.52 -5.05 3.42 6.95 0.79 4.09 4.17 4.42 0.39 -5.94 -7.06 -2.23 5.23 6.25
Nucleolus -4.67 -6.94 -5.14 -3.10 -4.02 0.03 0.80 -1.37 -1.59 -4.09 -2.61 -5.05 -7.46 -1.99 -5.72
Nucleus -8.68 -9.61 -8.98 5.89 0.15 -3.02 4.51 -0.97 2.95 -9.68 -16.00 -8.30 -14.45 7.42 -7.72
Peroxisomes -1.55 -1.06 -6.27 -3.81 -6.28 0.06 -0.92 -1.96 -2.06 -1.08 -1.86 -2.65 -6.93 -3.76 -4.57
Vacuole -6.83 -17.39 -27.99 -9.16 -11.81 0.97 -1.95 -6.34 -10.92 -7.39 -9.45 -21.65 -33.69 -15.91 -13.68
Vacuole Periphery 0.99 -4.13 1.40 0.76 5.89 2.18 1.45 3.00 1.89 5.27 0.93 -3.13 2.23 1.92 7.44
Standard name
Human Ortholog
Description Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Cdc19

Cdc19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available