Standard name
Human Ortholog
Description Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0 0 0 0 0 0 – 0 0 0 0.12 0.07 0.09 0.11 0.14 0.05 0.07 0.07 0.06 0.06 0.08 0.08
Bud 0 0 0.07 0 0 0 0.06 0 – 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.09 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0.11 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0.16 0.63 0.44 0.54 0.18 0.08 0.08 0.06 – 0.07 0 0.05 0.46 0.47 0.51 0.41 0.44 0.41 0.12 0.19 0.15 0.14 0.11 0.1
Endoplasmic Reticulum 0.21 0.14 0.09 0.07 0 0.07 0 0 – 0 0 0 0.28 0.21 0.15 0 0.05 0.07 0.11 0.08 0.07 0.1 0.1 0.05
Endosome 0 0 0.06 0 0.2 0 0.05 0 – 0 0 0 0 0.06 0.06 0.1 0.07 0.06 0 0.1 0.09 0.09 0.1 0.16
Golgi 0.14 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0.1 0.14 0 0 0 0.05 0.1
Mitochondria 0.53 0.05 0.41 0.46 0.59 0.83 0.82 0.85 – 0.85 0.93 0.93 0 0 0 0.06 0 0.09 0.36 0.3 0.51 0.33 0.24 0.17
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.06 0.08 0 0.2 0.12 0.19 0.12 – 0.14 0.09 0.08 0.14 0.21 0.22 0.23 0.21 0.3 0 0.11 0 0.13 0.17 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 7 4 4 8 0 0 – 1 2 1 19 16 21 19 24 8 5 12 13 10 19 26
Bud 0 3 13 1 11 9 30 19 – 21 26 40 1 1 3 0 2 3 3 3 1 4 11 26
Bud Neck 0 0 0 0 0 0 1 0 – 1 0 1 0 0 0 0 0 0 0 7 3 7 10 8
Bud Site 0 0 1 0 1 10 6 7 – 7 2 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 9 2 2 6 1 3 1 – 6 6 4 9 5 8 19 16 14 0 1 1 2 2 1
Cytoplasm 7 61 82 75 65 31 45 39 – 37 14 32 75 100 118 73 77 61 9 32 34 26 28 34
Endoplasmic Reticulum 9 14 17 10 7 30 1 4 – 8 6 6 45 45 34 5 8 10 8 13 16 19 24 16
Endosome 1 4 12 4 74 2 29 18 – 13 1 6 6 13 13 18 13 9 3 16 20 16 24 51
Golgi 6 1 0 0 2 1 1 0 – 0 2 1 0 8 7 18 14 15 10 3 2 7 13 34
Mitochondria 23 5 77 65 214 331 439 589 – 483 378 567 1 8 4 11 7 13 28 49 119 62 61 55
Nucleus 0 0 0 0 2 12 9 13 – 6 3 5 1 2 3 2 3 1 0 1 0 0 2 2
Nuclear Periphery 0 0 0 0 1 2 8 8 – 12 9 4 0 0 1 0 0 0 0 1 0 0 2 4
Nucleolus 3 0 0 2 5 4 13 21 – 12 9 6 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 2 1 – 2 1 0 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole 0 0 2 0 6 3 13 14 – 18 5 9 2 1 0 2 2 1 1 1 4 1 4 13
Vac/Vac Membrane 5 6 15 6 73 49 103 83 – 78 37 52 23 45 50 42 37 44 3 18 7 24 42 41
Unique Cell Count 43 97 186 140 364 401 537 690 566 408 612 163 215 230 180 175 147 80 166 234 188 253 329
Labelled Cell Count 55 113 228 169 472 493 703 817 705 501 740 182 244 262 210 203 180 80 166 234 188 253 329


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.1 4.9 5.2 5.9 4.9 4.5 4.4 – 4.5 4.3 4.5 8.3 8.1 8.0 9.6 9.8 10.4 9.0 9.6 10.5
Std Deviation (1e-4) 2.7 0.9 0.8 1.0 1.3 0.8 1.0 0.9 – 0.9 0.9 0.9 1.1 1.0 1.1 1.6 1.7 2.0 1.5 1.9 2.3
Intensity Change (Log2) – – – 0.09 0.26 0.02 -0.13 -0.14 – -0.11 -0.19 -0.12 0.77 0.73 0.72 0.98 1.0 1.09 0.88 0.98 1.11

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.8 1.6 2.2 2.5 3.4 0.7
Bud -2.8 -2.2 -2.8 0 0 – 0 0 0 -3.0 -3.5 -3.0 -3.6 -2.8 -2.1
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 2.4 0 0 3.9 3.5 3.6
Cytoplasm 1.7 -6.6 -10.4 -11.0 -13.5 – -12.2 -12.5 -13.3 0.4 0.5 1.5 -0.7 0 -0.5
Endoplasmic Reticulum -0.6 -3.9 -0.7 0 0 – 0 0 0 4.5 3.3 1.7 -2.6 -1.7 -0.8
Endosome -1.5 4.2 0 0 0 – 0 0 0 -1.2 -0.2 -0.3 1.2 0.4 -0.1
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 4.4 3.9 4.5
Mitochondria 0.9 3.9 10.1 0 0 – 0 0 0 -9.1 -9.2 -10.2 -7.9 -8.4 -6.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.4 3.6 1.5 3.5 1.5 – 2.1 0.4 0.2 1.8 3.6 3.8 4.0 3.5 5.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6716 4.7763 4.1438 3.6913 3.3166 4.3093 5.6029 6.591 6.035 5.8547 5.1877 6.26 4.5631 5.1598 4.9637 4.0565 4.1315 4.7818
Actin 0.0202 0.0151 0.0287 0.0427 0.0273 0.0183 0.0495 0.0175 0.0374 0.0659 0.0384 0.0313 0.0269 0.0156 0.0332 0.0198 0.0185 0.0194
Bud 0.0026 0.0046 0.012 0.0081 0.0087 0.0031 0.0035 0.0033 0.0081 0.0197 0.0096 0.0033 0.0036 0.0062 0.0085 0.0197 0.0098 0.0052
Bud Neck 0.0042 0.0021 0.0032 0.0032 0.0091 0.0099 0.0027 0.0028 0.007 0.003 0.0026 0.0158 0.0045 0.0044 0.0039 0.0037 0.0058 0.0138
Bud Periphery 0.003 0.0029 0.0163 0.0184 0.0134 0.0035 0.0042 0.0023 0.0114 0.0489 0.0093 0.0045 0.0048 0.005 0.0087 0.0274 0.0131 0.0081
Bud Site 0.0142 0.0526 0.0258 0.0041 0.0068 0.0039 0.0217 0.0388 0.0335 0.0079 0.0071 0.003 0.0201 0.0499 0.0262 0.0136 0.0064 0.0033
Cell Periphery 0.0021 0.0019 0.0056 0.0092 0.0014 0.0009 0.0041 0.0033 0.0071 0.0031 0.0013 0.0023 0.0041 0.0029 0.0048 0.0018 0.0029 0.0015
Cytoplasm 0.4055 0.5184 0.4807 0.5762 0.4202 0.4676 0.3132 0.5629 0.4477 0.4751 0.3769 0.4032 0.278 0.5326 0.421 0.4486 0.4474 0.3916
Cytoplasmic Foci 0.1234 0.1131 0.1057 0.0632 0.1561 0.1212 0.1018 0.0964 0.0972 0.0678 0.1103 0.1094 0.1162 0.0806 0.1092 0.1009 0.1154 0.1112
Eisosomes 0.0005 0.0002 0.0007 0.0004 0.0003 0.0001 0.0009 0.0002 0.001 0.0005 0.0003 0.0003 0.0005 0.0002 0.0005 0.0002 0.0001 0.0002
Endoplasmic Reticulum 0.0289 0.0299 0.0276 0.0178 0.0054 0.0342 0.0803 0.0293 0.0367 0.0217 0.032 0.0303 0.0817 0.04 0.037 0.0297 0.02 0.0386
Endosome 0.2015 0.1592 0.133 0.1144 0.1472 0.1845 0.1671 0.1074 0.1073 0.0822 0.1437 0.1488 0.2479 0.1377 0.1485 0.1179 0.1167 0.1974
Golgi 0.0594 0.0566 0.0583 0.0436 0.0772 0.0629 0.0915 0.0695 0.0921 0.0647 0.1206 0.0873 0.0802 0.0589 0.0889 0.063 0.0939 0.0929
Lipid Particles 0.0092 0.0013 0.0038 0.0017 0.003 0.0037 0.0181 0.0013 0.0028 0.002 0.0096 0.0052 0.0117 0.0044 0.0063 0.0083 0.0049 0.004
Mitochondria 0.0444 0.0108 0.0535 0.0679 0.0585 0.0485 0.0674 0.0427 0.0851 0.1201 0.1005 0.1155 0.052 0.0369 0.0756 0.0908 0.0949 0.0823
None 0.0321 0.0127 0.0211 0.0078 0.0328 0.0104 0.0298 0.0073 0.0029 0.0037 0.0019 0.0165 0.0102 0.003 0.0034 0.0042 0.0031 0.0045
Nuclear Periphery 0.0051 0.0029 0.0025 0.0026 0.0011 0.003 0.0081 0.0016 0.0016 0.0012 0.0018 0.0011 0.0121 0.0025 0.0022 0.0023 0.0013 0.0036
Nucleolus 0.0005 0.0002 0.0004 0.0006 0.0025 0.0002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0008 0.0002 0.0003 0.0013 0.0002 0.0002
Nucleus 0.0029 0.0024 0.0023 0.0035 0.0038 0.0024 0.003 0.0017 0.0014 0.0012 0.001 0.0013 0.0053 0.0024 0.0022 0.0028 0.0018 0.0028
Peroxisomes 0.0157 0.0035 0.0079 0.0042 0.0114 0.0085 0.015 0.0039 0.0084 0.0034 0.0222 0.011 0.0083 0.0029 0.0075 0.0255 0.031 0.0061
Punctate Nuclear 0.0042 0.0007 0.0018 0.0006 0.0054 0.0015 0.0041 0.0006 0.0006 0.0004 0.0005 0.0006 0.0042 0.0007 0.001 0.0036 0.0014 0.0017
Vacuole 0.0167 0.007 0.0071 0.0066 0.0066 0.0083 0.0106 0.0053 0.0084 0.0057 0.0061 0.0065 0.0219 0.0108 0.0082 0.0109 0.008 0.008
Vacuole Periphery 0.0039 0.0019 0.0022 0.0033 0.0017 0.0033 0.0032 0.0016 0.002 0.0018 0.0041 0.0027 0.0051 0.0021 0.003 0.0037 0.0033 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3467 34.097 31.6268 24.0485 15.0126 32.6754 21.2393 30.3322 43.5955 21.3647
Translational Efficiency 2.2908 1.3117 1.5109 2.3992 2.0289 1.3433 2.4583 2.309 1.5641 1.7537

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1865 1845 1239 1512 2478 1266 1447 1158 4343 3111 2686 2670

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 797.74 900.73 995.98 1128.87 820.24 841.29 1124.83 1126.87 810.58 876.54 1065.39 1128.00
Standard Deviation 104.55 126.77 132.75 170.82 107.56 128.76 142.39 209.30 106.86 130.88 152.24 188.48
Intensity Change Log 2 — 0.175176 0.320198 0.500889 — 0.036557 0.455589 0.458203 — 0.106567 0.390422 0.479407

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000942 0.004681 0.006428 0.021188 0.001063 0.003991 0.012753 0.026584 0.001011 0.004401 0.009835 0.023528
Bud Neck 0.025500 0.024360 0.018337 0.008961 0.029270 0.027620 0.024071 0.008257 0.027651 0.025687 0.021426 0.008655
Bud Site 0.045576 0.103082 0.082633 0.162172 0.045945 0.087234 0.197798 0.217330 0.045787 0.096633 0.144674 0.186095
Cell Periphery 0.000337 0.000536 0.000282 0.000311 0.000210 0.000754 0.000321 0.000539 0.000265 0.000625 0.000303 0.000410
Cytoplasm 0.078640 0.103832 0.286530 0.118894 0.151528 0.096374 0.209811 0.132106 0.120228 0.100797 0.245200 0.124624
Cytoplasmic Foci 0.230703 0.268771 0.205990 0.152872 0.324954 0.247193 0.122580 0.171469 0.284480 0.259990 0.161055 0.160938
Eisosomes 0.000279 0.000302 0.000099 0.000083 0.000237 0.000449 0.000099 0.000090 0.000255 0.000362 0.000099 0.000086
Endoplasmic Reticulum 0.009085 0.005515 0.014477 0.002956 0.009818 0.005344 0.001957 0.003294 0.009503 0.005445 0.007733 0.003103
Endosome 0.112483 0.106002 0.057680 0.053071 0.092668 0.125235 0.060825 0.064045 0.101177 0.113829 0.059374 0.057831
Golgi 0.098282 0.121939 0.019604 0.063975 0.068615 0.127103 0.049486 0.099205 0.081355 0.124041 0.035702 0.079255
Lipid Particles 0.015716 0.006787 0.009906 0.004684 0.016010 0.011893 0.002020 0.004839 0.015884 0.008865 0.005658 0.004751
Mitochondria 0.260164 0.131049 0.007838 0.023560 0.110334 0.147612 0.057882 0.023937 0.174675 0.137789 0.034797 0.023724
Mitotic Spindle 0.003437 0.011592 0.030841 0.273777 0.004899 0.008381 0.054151 0.151131 0.004271 0.010285 0.043399 0.220584
None 0.000753 0.000384 0.000952 0.000188 0.000717 0.000426 0.000195 0.000272 0.000733 0.000401 0.000544 0.000225
Nuclear Periphery 0.000331 0.000146 0.001776 0.000322 0.000310 0.000175 0.000595 0.000123 0.000319 0.000158 0.001139 0.000236
Nuclear Periphery Foci 0.000484 0.000693 0.006322 0.002666 0.000525 0.000489 0.000716 0.001641 0.000507 0.000610 0.003302 0.002221
Nucleolus 0.000549 0.000872 0.001887 0.000214 0.000707 0.000716 0.000666 0.000165 0.000639 0.000808 0.001229 0.000193
Nucleus 0.005309 0.003873 0.043200 0.001711 0.012443 0.003129 0.004348 0.001363 0.009379 0.003570 0.022270 0.001560
Peroxisomes 0.012842 0.024330 0.015203 0.024572 0.012410 0.022887 0.019240 0.023171 0.012596 0.023743 0.017378 0.023964
Vacuole 0.065103 0.059195 0.180798 0.072109 0.094732 0.056686 0.156704 0.056205 0.082009 0.058174 0.167818 0.065211
Vacuole Periphery 0.033486 0.022056 0.009219 0.011715 0.022606 0.026311 0.023782 0.014233 0.027278 0.023788 0.017064 0.012807

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.51 -7.41 -10.00 -7.80 -6.65 -4.61 -7.73 -10.32 -8.84 -4.83 -8.59 -10.00 -14.34 -11.74 -7.49
Bud Neck 0.57 3.47 9.31 9.83 6.11 0.70 2.60 14.05 9.44 10.49 1.31 4.34 16.47 13.68 11.79
Bud Site -13.52 -7.77 -19.70 -8.76 -12.08 -9.55 -23.98 -23.84 -16.14 -2.58 -16.78 -23.57 -30.62 -17.22 -7.63
Cell Periphery -2.87 2.12 1.18 3.37 -0.40 -7.12 -2.67 -4.13 2.14 -2.55 -7.07 -1.47 -3.44 3.72 -2.33
Cytoplasm -5.22 -24.89 -8.06 -3.43 17.64 9.61 -8.55 1.87 -5.75 8.77 5.00 -22.80 -2.36 -6.36 18.74
Cytoplasmic Foci -6.18 4.71 13.65 19.36 7.30 11.75 37.00 24.43 11.05 -7.80 5.30 28.70 28.69 21.91 -0.19
Eisosomes -0.76 9.46 10.66 9.42 3.26 -3.73 13.69 15.99 6.38 1.04 -3.77 15.73 17.78 10.33 2.96
Endoplasmic Reticulum 2.85 -3.51 5.61 2.96 9.24 3.13 7.61 5.29 1.69 -1.46 4.31 1.90 7.83 3.27 6.77
Endosome 1.50 14.76 16.69 15.11 1.60 -7.20 10.05 8.83 13.80 -0.59 -4.10 17.37 18.32 20.28 1.04
Golgi -4.70 22.61 9.21 13.31 -11.83 -11.70 6.38 -4.91 5.28 -8.82 -12.39 19.46 2.55 13.00 -12.87
Lipid Particles 6.99 4.19 8.63 2.33 6.01 2.56 14.93 10.16 4.67 -4.95 7.35 12.75 13.47 5.09 1.41
Mitochondria 16.70 40.08 36.28 21.81 -6.52 -5.32 11.74 21.19 19.18 8.30 7.31 35.79 39.89 29.03 4.76
Mitotic Spindle -6.51 -10.55 -29.82 -28.71 -25.70 -2.32 -11.91 -18.72 -18.15 -10.74 -6.06 -15.32 -34.56 -33.40 -25.90
None 1.32 -0.56 2.03 3.88 5.00 1.96 4.56 3.52 1.56 -1.18 2.31 1.33 3.70 3.39 4.19
Nuclear Periphery 3.35 -4.39 -0.11 -2.54 4.31 2.68 -1.34 4.97 0.94 1.89 4.64 -3.94 1.32 -2.01 4.19
Nuclear Periphery Foci -1.55 -9.77 -3.59 -3.09 4.19 0.25 -1.97 -4.77 -4.89 -3.19 -1.30 -9.74 -4.90 -4.42 2.22
Nucleolus -1.21 -4.02 1.77 2.18 4.90 -0.08 0.00 6.36 2.75 1.68 -0.99 -2.71 4.96 3.22 4.47
Nucleus 1.69 -15.91 3.63 2.68 17.40 12.16 8.60 15.45 3.62 5.94 10.25 -11.09 13.68 4.18 17.29
Peroxisomes -7.73 -1.53 -6.31 0.31 -4.87 -7.58 -4.30 -6.66 0.15 -1.94 -10.92 -4.40 -9.15 0.36 -4.58
Vacuole 1.49 -19.12 -3.90 -5.04 14.77 9.37 -11.15 7.74 -1.02 15.84 8.66 -20.70 2.53 -4.64 20.90
Vacuole Periphery 6.78 16.49 13.32 7.67 -1.73 -2.26 -0.01 5.84 6.81 4.62 3.10 9.50 13.66 10.17 3.51
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
Localization
Cell Percentages mitochondrion (45%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cyc3

Cyc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available