Standard name
Human Ortholog
Description Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.91 0.94 0.99 0.98 0.97 0.91 0.9 0.76 0.72 0.73 0.67 0.65 1.0 0.99 1.0 1.0 0.97 – 0.82 0.87 0.84 0.92 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.18 0.07 0 0 0 0.05 0.07 0.06 0.34 0.23 0.29 0.26 0 0 0 0.11 0 – 0.08 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.06 0.18 0.06 0.11 0.12 0.15 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 – 1 0 0 0 2 1
Bud 3 1 0 0 1 1 1 2 4 2 7 7 0 0 0 0 0 – 0 0 0 0 8 5
Bud Neck 0 1 3 2 1 3 4 5 2 0 1 3 0 2 0 0 0 – 0 2 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 1 3 2 1 2 1 1 3 2 4 5 2 2 0 0 2 – 0 0 0 0 1 0
Cytoplasm 108 188 147 254 304 315 298 400 249 251 213 261 260 361 315 18 61 – 116 170 130 141 288 268
Endoplasmic Reticulum 2 1 0 1 1 1 0 2 1 0 0 2 0 0 0 0 4 – 0 1 0 0 0 2
Endosome 0 1 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 – 2 3 2 0 2 2
Golgi 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 1 2 0 2 0
Mitochondria 21 13 0 0 1 18 24 29 117 80 93 103 0 0 0 2 1 – 11 8 6 3 3 5
Nucleus 0 0 0 0 4 12 9 12 14 7 17 9 1 2 0 0 1 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 3 4 1 2 3 0 0 0 0 0 – 0 0 1 0 0 0
Nucleolus 0 1 0 1 0 0 0 0 1 0 0 1 0 1 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 4 2 0 1 1 2 0 0 0 0 0 – 4 1 1 0 4 5
Vac/Vac Membrane 0 0 0 0 8 21 20 93 22 37 37 59 0 0 0 0 2 – 1 2 3 0 2 3
Unique Cell Count 119 200 148 259 313 347 332 523 344 342 318 399 261 363 315 18 63 143 197 156 153 323 305
Labelled Cell Count 134 207 153 262 322 373 361 550 418 382 376 459 263 368 315 20 71 143 197 156 153 323 305


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.5 5.3 5.1 6.0 5.4 5.7 6.0 5.0 5.6 5.8 6.1 4.9 4.9 4.8 10.5 13.4 – 5.7 5.8 5.9
Std Deviation (1e-4) 0.7 0.8 1.5 1.9 2.0 2.0 2.3 2.0 2.1 2.2 2.3 2.6 1.0 0.8 1.1 3.8 3.7 – 1.9 2.1 2.4
Intensity Change (Log2) – – – -0.06 0.18 0.03 0.12 0.19 -0.07 0.08 0.13 0.2 -0.12 -0.12 -0.14 0.99 1.34 – 0.1 0.13 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6761 -0.3147 -0.5989 -0.7172 -0.6812 -0.5129 3.6026 3.7014 3.2799 2.9479 3.3129 3.6208 0.1242 0.4203 0.3847 0.1437 -0.0371 0.4405
Actin 0.0254 0.0009 0.0034 0.0069 0.0012 0.0005 0.0453 0.0006 0.0068 0.0051 0.0134 0.0084 0.0209 0.0002 0.0033 0.0334 0.0013 0.0018
Bud 0.0012 0.0002 0.0015 0.001 0.0001 0.0001 0.0031 0.0022 0.002 0.0014 0.0026 0.0013 0.0002 0.0007 0.0011 0.0001 0.0003 0.0018
Bud Neck 0.0012 0.0001 0.0004 0.0001 0.0001 0.0004 0.001 0.0001 0.0027 0.0001 0.0004 0.0004 0.0043 0.0001 0.0003 0.0008 0.0001 0.0003
Bud Periphery 0.0033 0.0001 0.0038 0.0034 0.0005 0.0001 0.0046 0.003 0.0084 0.0047 0.0091 0.0038 0.0003 0.0003 0.0015 0.0001 0.0006 0.0052
Bud Site 0.0046 0.0005 0.0029 0.0002 0 0 0.012 0.0139 0.0011 0.0003 0.0046 0.0001 0.0031 0.0013 0.0016 0.0001 0.002 0.0002
Cell Periphery 0.0006 0.0001 0.0001 0 0 0 0.0008 0.0002 0.0001 0.0001 0.0003 0 0.0002 0.0002 0.0001 0 0.0001 0.0001
Cytoplasm 0.2068 0.2754 0.2564 0.258 0.275 0.3288 0.1112 0.2482 0.1601 0.129 0.1537 0.2527 0.1689 0.1877 0.1734 0.224 0.1578 0.2853
Cytoplasmic Foci 0.0148 0.0062 0.0089 0.0112 0.0033 0.013 0.0266 0.005 0.0176 0.0042 0.0258 0.0066 0.0099 0.0055 0.0101 0.0042 0.0061 0.0052
Eisosomes 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0032 0.0038 0.0021 0.0026 0.0017 0.001 0.0103 0.0007 0.0009 0.0008 0.0035 0.0004 0.003 0.001 0.0009 0.0007 0.0004 0.002
Endosome 0.0088 0.0021 0.0035 0.0031 0.0054 0.0019 0.0338 0.0004 0.0044 0.0004 0.0385 0.0024 0.008 0.0005 0.0016 0.0007 0.0031 0.0021
Golgi 0.0043 0.0002 0.0003 0.0009 0.0002 0.0001 0.0167 0.0001 0.0039 0.0002 0.0324 0.0028 0.0046 0.0001 0.0011 0.0004 0.0019 0.0003
Lipid Particles 0.0062 0.0006 0.0004 0.0005 0.0002 0.0003 0.0176 0.0001 0.0023 0.0001 0.0279 0.0028 0.0098 0.0002 0.0033 0.0001 0.0094 0.0012
Mitochondria 0.0047 0.0003 0.0006 0.0049 0.0432 0.0002 0.0112 0.0002 0.0097 0.0004 0.0063 0.001 0.0016 0.0006 0.0072 0.0002 0.0027 0.0019
None 0.6945 0.6995 0.7037 0.6869 0.6316 0.6485 0.6637 0.723 0.7659 0.8503 0.6426 0.7078 0.7457 0.7971 0.7806 0.7324 0.7974 0.6837
Nuclear Periphery 0.0045 0.0018 0.0016 0.002 0.0284 0.0006 0.0142 0.0002 0.0005 0.0003 0.0053 0.0005 0.0064 0.0002 0.0004 0.0003 0.0006 0.0011
Nucleolus 0.0005 0.0002 0.0005 0.0001 0.0002 0.0001 0.0008 0.0001 0.0001 0.0001 0.0012 0.0001 0.0003 0.0005 0.0002 0.0001 0.0015 0.0002
Nucleus 0.0021 0.0024 0.005 0.0023 0.0052 0.0018 0.0033 0.0007 0.0011 0.0003 0.0023 0.0005 0.0026 0.0011 0.0009 0.0008 0.0009 0.0008
Peroxisomes 0.0077 0.0001 0.0006 0.0138 0.0002 0.0001 0.0077 0.0003 0.011 0.0014 0.0181 0.0075 0.0066 0.0001 0.0085 0.0001 0.0077 0.0008
Punctate Nuclear 0.003 0.0044 0.0031 0.0007 0.002 0.002 0.0111 0.0003 0.0007 0.0004 0.0051 0.0006 0.002 0.0022 0.003 0.0009 0.0057 0.0051
Vacuole 0.0013 0.0008 0.001 0.0009 0.0005 0.0004 0.0027 0.0004 0.0005 0.0002 0.0054 0.0002 0.0008 0.0004 0.0005 0.0002 0.0004 0.0005
Vacuole Periphery 0.0008 0.0002 0.0002 0.0002 0.0009 0.0001 0.002 0 0.0002 0 0.0014 0.0001 0.0004 0 0.0001 0 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.3949 39.9343 26.6696 38.4618 42.4743 26.7885 40.714 43.6033 35.5634 46.3917
Translational Efficiency 1.4496 0.989 1.5047 1.0567 1.245 2.5135 0.9656 1.0247 1.4523 1.2069

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1455 1188 1431 1786 2343 1559 1260 1778 3798 2747 2691 3564

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 627.83 722.04 810.46 875.73 641.18 771.69 814.13 818.81 636.07 750.22 812.18 847.33
Standard Deviation 77.71 97.97 94.91 157.13 78.19 97.07 113.43 140.00 78.28 100.52 104.01 151.53
Intensity Change Log 2 — 0.201705 0.368367 0.480112 — 0.267292 0.344530 0.352799 — 0.235216 0.356372 0.417190

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000178 0.000451 0.000097 0.001902 0.000023 0.000252 0.000190 0.000152 0.000082 0.000338 0.000141 0.001029
Bud Neck 0.020214 0.033508 0.005189 0.010203 0.005103 0.038759 0.003629 0.003899 0.010892 0.036488 0.004459 0.007058
Bud Site 0.005530 0.018078 0.008242 0.036645 0.001306 0.015347 0.007629 0.026022 0.002924 0.016528 0.007955 0.031345
Cell Periphery 0.000324 0.000147 0.000083 0.000189 0.000089 0.000352 0.000090 0.000186 0.000179 0.000263 0.000086 0.000188
Cytoplasm 0.467956* 0.424560* 0.547676* 0.472110* 0.621802* 0.386546* 0.477863* 0.588915* 0.562864* 0.402986* 0.514988* 0.530381*
Cytoplasmic Foci 0.298052* 0.265149* 0.009794 0.021835 0.098634 0.201326* 0.008602 0.022725 0.175030 0.228928* 0.009236 0.022279
Eisosomes 0.000422 0.000137 0.000017 0.000059 0.000046 0.000196 0.000026 0.000040 0.000190 0.000171 0.000021 0.000050
Endoplasmic Reticulum 0.001535 0.000825 0.001110 0.001369 0.000279 0.001125 0.002088 0.000775 0.000760 0.000995 0.001568 0.001072
Endosome 0.008190 0.023920 0.001098 0.012594 0.001329 0.026995 0.001336 0.003214 0.003957 0.025665 0.001209 0.007915
Golgi 0.006233 0.004986 0.000046 0.003895 0.000321 0.004295 0.000332 0.000573 0.002586 0.004594 0.000179 0.002238
Lipid Particles 0.013290 0.006601 0.000574 0.001760 0.002258 0.011357 0.000806 0.000910 0.006484 0.009300 0.000683 0.001336
Mitochondria 0.013596 0.011150 0.000610 0.004460 0.002083 0.015433 0.001507 0.000842 0.006494 0.013581 0.001030 0.002655
Mitotic Spindle 0.000135 0.006174 0.003956 0.074512 0.000566 0.005387 0.002461 0.020118 0.000400 0.005727 0.003256 0.047376
None 0.007905 0.002724 0.003533 0.007322 0.006807 0.005254 0.008012 0.009218 0.007227 0.004159 0.005630 0.008268
Nuclear Periphery 0.000340 0.000422 0.001976 0.001400 0.000227 0.000790 0.001281 0.000923 0.000270 0.000631 0.001650 0.001162
Nuclear Periphery Foci 0.000254 0.001263 0.000342 0.001059 0.000099 0.000410 0.001102 0.000548 0.000158 0.000779 0.000698 0.000804
Nucleolus 0.001566 0.002076 0.000268 0.001944 0.000569 0.001936 0.000335 0.000603 0.000951 0.001997 0.000299 0.001275
Nucleus 0.123699 0.102446 0.375195* 0.219531* 0.246793* 0.179468* 0.435662* 0.243893* 0.199636* 0.146158* 0.403507* 0.231685*
Peroxisomes 0.007325 0.017480 0.000172 0.003817 0.000623 0.012861 0.000131 0.000428 0.003190 0.014858 0.000153 0.002126
Vacuole 0.021647 0.075366 0.039863 0.118002 0.010721 0.089095 0.046797 0.074421 0.014907 0.083157 0.043110 0.096261
Vacuole Periphery 0.001608 0.002536 0.000160 0.005391 0.000323 0.002816 0.000122 0.001595 0.000815 0.002695 0.000142 0.003497

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.36 1.63 -2.09 -1.24 -2.23 -6.63 -13.59 -11.30 2.13 3.89 -4.78 -7.06 -2.63 -1.11 -2.01
Bud Neck -4.58 9.97 9.00 10.22 -2.01 -13.50 1.37 1.91 14.26 -0.05 -13.68 7.90 7.63 17.34 -1.50
Bud Site -7.30 -0.86 -7.25 -0.76 -5.71 -9.00 -3.50 -8.32 -1.37 -5.14 -11.84 -3.59 -11.49 -1.62 -7.63
Cell Periphery 7.34 11.03 9.36 1.98 -3.47 -5.37 -1.20 -6.90 4.16 -5.82 -2.85 9.65 4.73 4.55 -6.26
Cytoplasm 4.29 -1.41 6.14 1.13 7.24 28.45 24.99 11.92 -14.50 -12.46 24.22 18.10 15.22 -9.16 -3.46
Cytoplasmic Foci 3.86 49.43 48.68 38.42 -3.63 -18.23 34.19 28.05 35.19 -9.74 -10.54 52.11 49.09 51.63 -9.49
Eisosomes 9.72 13.97 12.85 11.37 -6.43 -13.32 4.01 1.22 13.59 -3.02 1.49 14.08 12.31 17.01 -7.29
Endoplasmic Reticulum 2.46 -0.90 -0.94 -8.33 -0.07 -3.35 -16.83 -12.57 -0.13 11.70 -1.34 -12.03 -6.06 -3.11 9.33
Endosome -9.53 10.76 3.08 11.14 -5.65 -16.47 -1.34 -3.99 15.37 -3.10 -19.20 9.58 0.08 18.61 -6.45
Golgi 1.65 9.71 6.67 5.62 -3.59 -7.59 0.88 -0.41 7.26 -0.92 -4.41 9.62 5.46 8.81 -3.66
Lipid Particles 8.10 18.73 17.38 10.28 -5.13 -9.30 7.18 7.83 10.73 0.60 -4.34 19.34 17.84 13.63 -4.19
Mitochondria 1.42 10.28 8.45 5.66 -3.04 -7.72 1.58 3.11 8.77 0.57 -5.47 9.31 8.26 10.28 -1.71
Mitotic Spindle -3.77 -4.96 -13.26 -10.70 -11.57 -4.13 -3.27 -7.01 -4.48 -5.22 -5.53 -5.65 -14.80 -11.50 -12.47
None 4.26 3.77 0.45 -5.86 -5.22 1.67 0.89 -0.33 -1.60 -0.86 4.29 4.55 0.06 -4.17 -4.35
Nuclear Periphery -1.24 -19.38 -22.75 -20.79 1.95 -5.79 -26.10 -20.49 -5.06 6.44 -5.82 -28.98 -30.66 -16.50 5.03
Nuclear Periphery Foci -1.58 -3.63 -8.67 0.40 -6.09 -3.30 -10.33 -7.74 -2.20 5.71 -2.22 -11.27 -12.19 -0.21 1.76
Nucleolus -1.30 3.53 1.30 3.46 -4.35 -6.52 3.44 1.16 6.82 -2.35 -4.93 4.33 1.03 6.64 -4.88
Nucleus 3.82 -36.73 -17.78 -20.13 19.61 9.99 -25.48 -3.14 -11.52 21.07 11.34 -39.60 -10.85 -20.29 28.47
Peroxisomes -6.28 14.89 9.37 10.08 -2.89 -10.06 8.53 3.58 10.29 -3.66 -11.94 15.56 8.14 14.25 -3.35
Vacuole -16.67 -13.30 -33.29 -21.85 -23.70 -21.69 -18.28 -26.66 -7.08 -6.67 -27.56 -23.14 -41.97 -20.76 -22.10
Vacuole Periphery -2.55 8.35 -0.58 1.09 -3.90 -9.35 5.32 -1.97 4.17 -2.60 -8.87 9.43 -2.16 3.52 -4.67
Standard name
Human Ortholog
Description Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor
Localization
Cell Percentages cytoplasm (33%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aim2

Aim2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available