Standard name | |
---|---|
Human Ortholog | |
Description | Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 63 | 79 | 36 | 40 | 32 | 38 | 39 | 70 | – | 35 | 56 | 60 | 8 | 40 | 19 | 44 | 21 | 46 | 80 | 59 | 160 | 21 | 78 | 66 |
Bud | 28 | 39 | 48 | 41 | 50 | 60 | 56 | 83 | – | 64 | 55 | 56 | 2 | 22 | 6 | 40 | 9 | 24 | 71 | 57 | 59 | 62 | 80 | 75 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 3 | 1 | 48 | 40 | 45 | 61 | 44 | 23 |
Bud Site | 17 | 15 | 27 | 12 | 13 | 28 | 17 | 23 | – | 21 | 15 | 14 | 1 | 1 | 0 | 7 | 4 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 2 | 13 | 13 | 2 | 8 | 8 | 3 | – | 15 | 10 | 17 | 0 | 7 | 3 | 4 | 2 | 3 | 2 | 1 | 3 | 0 | 2 | 4 |
Cytoplasm | 92 | 84 | 103 | 69 | 60 | 78 | 90 | 113 | – | 127 | 96 | 110 | 5 | 99 | 110 | 128 | 61 | 34 | 35 | 24 | 17 | 5 | 29 | 39 |
Endoplasmic Reticulum | 32 | 21 | 16 | 24 | 29 | 44 | 51 | 49 | – | 50 | 37 | 62 | 5 | 22 | 13 | 13 | 11 | 6 | 17 | 10 | 30 | 3 | 12 | 14 |
Endosome | 10 | 1 | 30 | 12 | 36 | 50 | 64 | 108 | – | 81 | 33 | 46 | 0 | 0 | 1 | 11 | 15 | 12 | 14 | 10 | 17 | 13 | 9 | 7 |
Golgi | 0 | 0 | 2 | 1 | 3 | 2 | 3 | 2 | – | 1 | 2 | 8 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 5 | 13 | 1 | 2 | 3 |
Mitochondria | 30 | 45 | 46 | 42 | 44 | 179 | 176 | 248 | – | 261 | 265 | 246 | 2 | 0 | 0 | 34 | 17 | 15 | 38 | 31 | 61 | 19 | 26 | 42 |
Nucleus | 3 | 1 | 3 | 3 | 2 | 3 | 5 | 7 | – | 3 | 3 | 0 | 1 | 3 | 2 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | – | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 12 | 10 | 33 | – | 14 | 15 | 9 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Peroxisomes | 31 | 14 | 100 | 38 | 68 | 45 | 60 | 53 | – | 32 | 17 | 26 | 0 | 0 | 1 | 20 | 10 | 5 | 2 | 0 | 7 | 3 | 4 | 1 |
SpindlePole | 10 | 3 | 18 | 7 | 5 | 8 | 9 | 13 | – | 9 | 6 | 6 | 0 | 4 | 0 | 40 | 18 | 18 | 5 | 2 | 4 | 3 | 4 | 1 |
Vac/Vac Membrane | 9 | 0 | 11 | 3 | 10 | 7 | 12 | 25 | – | 12 | 2 | 12 | 0 | 0 | 1 | 3 | 3 | 3 | 13 | 13 | 6 | 12 | 10 | 23 |
Unique Cell Count | 250 | 213 | 337 | 222 | 272 | 413 | 465 | 624 | 549 | 457 | 504 | 16 | 164 | 140 | 285 | 144 | 123 | 338 | 264 | 437 | 214 | 308 | 310 | |
Labelled Cell Count | 332 | 305 | 454 | 306 | 356 | 562 | 600 | 832 | 726 | 616 | 674 | 24 | 199 | 156 | 355 | 179 | 168 | 338 | 264 | 437 | 214 | 308 | 310 |
Ambiguous, Bud, Bud Neck, Cell Periphery, Late Golgi, Punctate Nuclear
(Huh et al. Nature 2003 425:686)Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.4 | 5.5 | 6.2 | 6.2 | 5.9 | 6.0 | 6.2 | – | 6.0 | 6.4 | 6.6 | 5.8 | 6.5 | 7.0 | 4.9 | 5.3 | 6.4 | 6.5 | 6.9 | 7.0 |
Std Deviation (1e-4) | 1.0 | 0.9 | 1.3 | 2.2 | 1.5 | 1.4 | 1.6 | 1.5 | – | 1.3 | 1.4 | 1.4 | 0.6 | 0.8 | 1.1 | 1.0 | 0.9 | 1.2 | 1.4 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.18 | 0.1 | 0.13 | 0.18 | – | 0.13 | 0.21 | 0.26 | 0.09 | 0.25 | 0.34 | -0.16 | -0.05 | 0.23 | 0.24 | 0.32 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | 1.3 | 1.4 | 0.1 | -0.9 | -0.4 | – | -1.1 | -0.9 | -1.4 | – | -0.3 | -3.1 | -0.1 | -2.5 | 1.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.2 | -1.6 | -0.6 | -2.7 | -2.9 | – | -2.7 | -2.9 | -3.5 | – | -3.4 | -3.4 | -3.0 | -2.1 | -3.2 |
Cell Periphery | 1.1 | -2.5 | -1.6 | -1.9 | -3.9 | – | -0.9 | -1.4 | -0.4 | – | 0.2 | 0 | -1.9 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | 2.7 | 2.8 | 3.0 | 3.1 | 1.8 | – | 2.4 | 1.9 | 3.7 | – | 3.4 | 1.9 | -0.1 | 1.3 | 0.1 |
Endosome | -1.5 | 1.7 | 1.4 | 2.1 | 3.5 | – | 2.6 | -0.9 | 0.1 | – | -3.9 | -3.3 | -2.5 | 0.5 | 0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | 0.9 | 8.8 | 7.6 | 8.4 | – | 10.3 | 12.7 | 10.5 | – | -5.0 | -4.6 | -0.6 | -0.5 | -0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | 0 | 0 | 2.7 | 0 | 4.0 | – | 2.5 | 3.0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -3.4 | -1.3 | -6.5 | -5.9 | -8.6 | – | -9.7 | -10.2 | -9.8 | – | -7.8 | -7.0 | -7.1 | -5.4 | -5.8 |
SpindlePole | -1.2 | -2.3 | -2.5 | -2.6 | -2.7 | – | -3.1 | -3.3 | -3.5 | – | -1.5 | -2.8 | 3.7 | 2.7 | 3.3 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | 2.5 | 0.4 | -0.7 | -1.1 | 0.3 | – | -2.3 | 0.7 | 0.5 | – | 4.0 | 0.9 | 1.8 | 1.2 | 6.6 |
Cytoplasm | 0.1 | -2.4 | -3.7 | -3.7 | -4.4 | – | -2.4 | -3.1 | -2.9 | – | 6.4 | 9.6 | 3.7 | 2.5 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.4 | 0.3 | -1.4 | -0.6 | 0.6 | – | -1.0 | -3.1 | -0.8 | – | 0 | 0 | -1.9 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.515 | 3.5681 | 4.3332 | 4.7227 | 4.0837 | 4.3534 | 5.3976 | 5.7937 | 5.2968 | 5.3513 | 4.9447 | 5.7873 | 6.1946 | 6.3277 | 6.1961 | 5.6617 | 5.455 | 5.895 |
Actin | 0.0952 | 0.0638 | 0.046 | 0.0497 | 0.1352 | 0.1016 | 0.1815 | 0.0725 | 0.0719 | 0.1544 | 0.3098 | 0.1132 | 0.2993 | 0.0789 | 0.0446 | 0.1374 | 0.01 | 0.1419 |
Bud | 0.0662 | 0.0486 | 0.2888 | 0.169 | 0.0745 | 0.0146 | 0.0592 | 0.0849 | 0.2196 | 0.0638 | 0.0419 | 0.0073 | 0.0012 | 0.0429 | 0.1691 | 0.0619 | 0.0932 | 0.0158 |
Bud Neck | 0.0486 | 0.0058 | 0.0054 | 0.0077 | 0.0436 | 0.5192 | 0.0743 | 0.0037 | 0.0273 | 0.0047 | 0.0103 | 0.5307 | 0.0042 | 0.0157 | 0.0453 | 0.0032 | 0.001 | 0.5851 |
Bud Periphery | 0.1167 | 0.0644 | 0.4115 | 0.4194 | 0.2152 | 0.0408 | 0.1475 | 0.0773 | 0.4737 | 0.2082 | 0.2012 | 0.0203 | 0.0006 | 0.0464 | 0.4615 | 0.1981 | 0.3858 | 0.0334 |
Bud Site | 0.1305 | 0.3287 | 0.0689 | 0.0194 | 0.0302 | 0.0281 | 0.0659 | 0.4592 | 0.0532 | 0.0223 | 0.0587 | 0.0129 | 0.3195 | 0.3604 | 0.0397 | 0.0188 | 0.0019 | 0.0302 |
Cell Periphery | 0.0158 | 0.0086 | 0.0061 | 0.0027 | 0.0068 | 0.0083 | 0.0203 | 0.0029 | 0.0027 | 0.005 | 0.0079 | 0.0035 | 0.0071 | 0.0029 | 0.0031 | 0.0061 | 0.001 | 0.0067 |
Cytoplasm | 0.1635 | 0.175 | 0.0327 | 0.0883 | 0.1484 | 0.0828 | 0.179 | 0.1562 | 0.0288 | 0.1908 | 0.0644 | 0.1378 | 0.14 | 0.2089 | 0.0837 | 0.1332 | 0.0122 | 0.0714 |
Cytoplasmic Foci | 0.0747 | 0.0746 | 0.0185 | 0.0306 | 0.0375 | 0.0561 | 0.0765 | 0.0426 | 0.0292 | 0.1243 | 0.0685 | 0.0527 | 0.0496 | 0.0685 | 0.0262 | 0.0999 | 0.0866 | 0.0325 |
Eisosomes | 0.001 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0018 | 0.0001 | 0.0009 | 0.0003 | 0.0012 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0233 | 0.0068 | 0.0037 | 0.0047 | 0.0148 | 0.0051 | 0.0181 | 0.0222 | 0.007 | 0.0038 | 0.0081 | 0.0061 | 0.0046 | 0.0245 | 0.0027 | 0.008 | 0.0007 | 0.0028 |
Endosome | 0.0949 | 0.1292 | 0.0484 | 0.0826 | 0.0875 | 0.0619 | 0.0495 | 0.0362 | 0.0162 | 0.1148 | 0.0987 | 0.0446 | 0.0648 | 0.039 | 0.0533 | 0.1317 | 0.0906 | 0.0149 |
Golgi | 0.0605 | 0.0552 | 0.028 | 0.032 | 0.084 | 0.044 | 0.051 | 0.0195 | 0.0106 | 0.0586 | 0.0761 | 0.0424 | 0.03 | 0.0822 | 0.0612 | 0.076 | 0.2908 | 0.0454 |
Lipid Particles | 0.0194 | 0.0051 | 0.0041 | 0.0022 | 0.0254 | 0.0049 | 0.0126 | 0.0007 | 0.0042 | 0.0058 | 0.0092 | 0.0047 | 0.056 | 0.0057 | 0.0009 | 0.0324 | 0.0012 | 0.0009 |
Mitochondria | 0.0198 | 0.0079 | 0.0096 | 0.0143 | 0.0325 | 0.0082 | 0.0128 | 0.0014 | 0.0214 | 0.0064 | 0.0136 | 0.0028 | 0.0003 | 0.003 | 0.0021 | 0.0416 | 0.0176 | 0.002 |
None | 0.0061 | 0.0056 | 0.0047 | 0.0021 | 0.0217 | 0.0015 | 0.0099 | 0.0037 | 0.0193 | 0.0017 | 0.0171 | 0.0039 | 0.0022 | 0.0038 | 0.0004 | 0.0027 | 0.0005 | 0.0003 |
Nuclear Periphery | 0.0075 | 0.0012 | 0.0015 | 0.0018 | 0.0082 | 0.0011 | 0.009 | 0.0016 | 0.0027 | 0.0011 | 0.0009 | 0.0004 | 0.0002 | 0.0028 | 0.0006 | 0.0013 | 0.0002 | 0.0002 |
Nucleolus | 0.0011 | 0.0005 | 0.0006 | 0.0003 | 0.0013 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0007 |
Nucleus | 0.0028 | 0.0015 | 0.0018 | 0.0019 | 0.0042 | 0.0024 | 0.0014 | 0.0011 | 0.0005 | 0.0009 | 0.0005 | 0.001 | 0.0003 | 0.0008 | 0.0004 | 0.001 | 0.0003 | 0.0022 |
Peroxisomes | 0.0175 | 0.0036 | 0.0055 | 0.0105 | 0.0063 | 0.0056 | 0.0193 | 0.0018 | 0.0041 | 0.0121 | 0.0049 | 0.0053 | 0.0012 | 0.0077 | 0.0008 | 0.0205 | 0.0044 | 0.0034 |
Punctate Nuclear | 0.0068 | 0.0012 | 0.0013 | 0.0008 | 0.0053 | 0.0009 | 0.0018 | 0.0004 | 0.0009 | 0.0014 | 0.0015 | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0073 | 0.0001 | 0.0003 |
Vacuole | 0.0247 | 0.0106 | 0.0104 | 0.0511 | 0.0133 | 0.01 | 0.0069 | 0.0108 | 0.0046 | 0.0158 | 0.0037 | 0.008 | 0.0172 | 0.0046 | 0.003 | 0.0137 | 0.0012 | 0.0094 |
Vacuole Periphery | 0.0033 | 0.0015 | 0.0024 | 0.0086 | 0.0038 | 0.002 | 0.0014 | 0.0009 | 0.0012 | 0.0037 | 0.0012 | 0.0008 | 0.0004 | 0.0006 | 0.001 | 0.0034 | 0.0007 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 75.5307 | 45.2928 | 33.6092 | 46.1305 | 62.346 | 46.4186 | 41.4492 | 37.4913 | 44.4437 | 75.498 |
Translational Efficiency | 0.8289 | 0.905 | 0.8488 | 0.7244 | 0.8536 | 1.3639 | 0.8931 | 0.7832 | 0.7413 | 0.7823 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
241 | 892 | 671 | 1184 | 2038 | 1624 | 334 | 1582 | 2279 | 2516 | 1005 | 2766 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 880.35 | 800.53 | 915.45 | 926.20 | 775.52 | 829.61 | 951.27 | 919.00 | 786.61 | 819.30 | 927.35 | 922.08 |
Standard Deviation | 136.12 | 99.99 | 112.76 | 130.76 | 93.23 | 99.54 | 92.91 | 121.40 | 103.79 | 100.67 | 107.90 | 125.54 |
Intensity Change Log 2 | — | -0.137122 | 0.056404 | 0.073247 | — | 0.097269 | 0.294691 | 0.244901 | — | -0.022594 | 0.172916 | 0.156188 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.018541 | 0.007258 | 0.003373 | 0.024973 | 0.009519 | 0.009343 | 0.006176 | 0.010274 | 0.010473 | 0.008604 | 0.004304 | 0.016566 |
Bud Neck | 0.034585 | 0.071547 | 0.081455 | 0.067966 | 0.144342 | 0.156326 | 0.116454 | 0.077068 | 0.132736 | 0.126269 | 0.093086 | 0.073172 |
Bud Site | 0.146065* | 0.225059* | 0.105886* | 0.251368* | 0.245219* | 0.293749* | 0.182488* | 0.227473* | 0.234734* | 0.269396* | 0.131344* | 0.237701* |
Cell Periphery | 0.006186 | 0.001359 | 0.000566 | 0.009204 | 0.001689 | 0.002145 | 0.000915 | 0.002883 | 0.002165 | 0.001866 | 0.000682 | 0.005588 |
Cytoplasm | 0.272068* | 0.252830* | 0.297630* | 0.296615* | 0.215081* | 0.231304* | 0.211322* | 0.312881* | 0.221107* | 0.238935* | 0.268947* | 0.305918* |
Cytoplasmic Foci | 0.066812 | 0.252281* | 0.150439 | 0.037257 | 0.181270* | 0.139985 | 0.089931 | 0.053793 | 0.169166 | 0.179798 | 0.130330 | 0.046715 |
Eisosomes | 0.000777 | 0.000840 | 0.000247 | 0.003252 | 0.002215 | 0.001895 | 0.000317 | 0.000844 | 0.002063 | 0.001521 | 0.000270 | 0.001875 |
Endoplasmic Reticulum | 0.009191 | 0.004816 | 0.007797 | 0.006221 | 0.003372 | 0.002165 | 0.009546 | 0.001883 | 0.003987 | 0.003105 | 0.008378 | 0.003740 |
Endosome | 0.012081 | 0.045185 | 0.032082 | 0.009454 | 0.040367 | 0.035783 | 0.017711 | 0.013506 | 0.037376 | 0.039116 | 0.027306 | 0.011772 |
Golgi | 0.008964 | 0.043848 | 0.003241 | 0.003954 | 0.054214 | 0.036734 | 0.000478 | 0.004750 | 0.049429 | 0.039256 | 0.002322 | 0.004409 |
Lipid Particles | 0.005332 | 0.009436 | 0.012877 | 0.002338 | 0.014639 | 0.006899 | 0.006942 | 0.001881 | 0.013655 | 0.007798 | 0.010905 | 0.002076 |
Mitochondria | 0.007986 | 0.016791 | 0.000726 | 0.003388 | 0.032809 | 0.033789 | 0.000366 | 0.005384 | 0.030184 | 0.027762 | 0.000606 | 0.004530 |
Mitotic Spindle | 0.003491 | 0.001664 | 0.002378 | 0.007906 | 0.001055 | 0.001305 | 0.002586 | 0.013430 | 0.001313 | 0.001432 | 0.002447 | 0.011066 |
None | 0.000162 | 0.000826 | 0.000847 | 0.000668 | 0.000319 | 0.000341 | 0.000388 | 0.000821 | 0.000302 | 0.000513 | 0.000694 | 0.000756 |
Nuclear Periphery | 0.000190 | 0.000057 | 0.001090 | 0.000226 | 0.000046 | 0.000033 | 0.000919 | 0.000158 | 0.000061 | 0.000042 | 0.001033 | 0.000187 |
Nuclear Periphery Foci | 0.001836 | 0.000378 | 0.007398 | 0.001206 | 0.000099 | 0.000090 | 0.006140 | 0.000644 | 0.000283 | 0.000192 | 0.006980 | 0.000885 |
Nucleolus | 0.000182 | 0.000203 | 0.000727 | 0.000123 | 0.000190 | 0.000148 | 0.000547 | 0.000198 | 0.000190 | 0.000167 | 0.000667 | 0.000166 |
Nucleus | 0.034192 | 0.005986 | 0.084474 | 0.046575 | 0.006776 | 0.004108 | 0.088445 | 0.036373 | 0.009675 | 0.004774 | 0.085794 | 0.040740 |
Peroxisomes | 0.009314 | 0.017904 | 0.010014 | 0.006523 | 0.018553 | 0.014393 | 0.009201 | 0.008403 | 0.017576 | 0.015638 | 0.009744 | 0.007598 |
Vacuole | 0.361208* | 0.036079 | 0.195654 | 0.219300* | 0.023680 | 0.024425 | 0.248664* | 0.224365* | 0.059373 | 0.028557 | 0.213271* | 0.222197* |
Vacuole Periphery | 0.000838 | 0.005656 | 0.001098 | 0.001484 | 0.004548 | 0.005040 | 0.000463 | 0.002988 | 0.004156 | 0.005258 | 0.000887 | 0.002344 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.18 | 2.04 | -1.45 | -5.87 | -8.36 | 0.13 | 2.43 | 0.19 | 0.06 | -2.22 | 1.07 | 5.03 | -2.97 | -4.36 | -8.31 |
Bud Neck | -5.65 | -5.05 | -3.80 | 2.88 | 2.54 | -1.46 | 4.38 | 12.47 | 12.80 | 2.35 | 0.84 | 6.78 | 13.18 | 12.87 | 3.08 |
Bud Site | -6.64 | 1.02 | -5.64 | 1.85 | -9.80 | -4.98 | 6.43 | 6.00 | 10.43 | -2.69 | -4.53 | 13.04 | 4.67 | 9.56 | -9.57 |
Cell Periphery | 1.01 | 1.29 | -0.91 | -5.74 | -6.77 | -2.08 | 5.87 | -1.51 | -0.45 | -3.61 | 0.26 | 4.06 | -4.13 | -4.99 | -7.78 |
Cytoplasm | -0.48 | -2.61 | -2.85 | -3.85 | -0.10 | -2.17 | 0.20 | -11.61 | -9.15 | -7.05 | -3.17 | -5.27 | -12.58 | -9.63 | -4.05 |
Cytoplasmic Foci | -20.60 | -10.54 | 3.93 | 26.48 | 15.99 | 6.38 | 11.19 | 24.26 | 16.40 | 4.65 | -1.93 | 6.32 | 26.71 | 29.25 | 15.28 |
Eisosomes | -1.48 | 2.38 | -4.12 | -3.72 | -5.12 | 1.31 | 9.63 | 6.79 | 6.80 | -4.23 | 2.58 | 10.00 | 2.09 | 0.15 | -6.17 |
Endoplasmic Reticulum | 0.53 | -0.32 | 0.20 | -1.18 | 2.19 | 3.02 | -4.18 | 3.91 | 0.67 | 5.21 | 1.03 | -5.90 | -0.03 | -1.40 | 6.75 |
Endosome | -10.81 | -7.80 | 1.33 | 12.25 | 9.52 | 1.86 | 10.42 | 14.29 | 11.06 | 2.50 | -0.91 | 5.07 | 16.33 | 16.60 | 9.00 |
Golgi | -9.85 | 1.10 | 1.10 | 14.62 | 0.02 | 5.82 | 23.86 | 20.91 | 15.19 | -3.77 | 3.80 | 22.19 | 21.30 | 20.96 | -1.61 |
Lipid Particles | -2.24 | -3.55 | 0.92 | 6.34 | 8.33 | 6.38 | 5.18 | 12.70 | 6.74 | 6.02 | 5.29 | 2.02 | 12.42 | 9.18 | 10.05 |
Mitochondria | -2.71 | 1.22 | 0.72 | 7.40 | -1.86 | -0.37 | 20.31 | 15.25 | 12.71 | -3.99 | 1.03 | 19.59 | 15.99 | 14.73 | -4.19 |
Mitotic Spindle | 1.24 | 0.54 | -2.02 | -3.87 | -3.13 | -0.59 | -2.37 | -5.56 | -5.38 | -4.24 | -0.41 | -3.06 | -6.71 | -6.58 | -5.39 |
None | -1.55 | -2.53 | -0.93 | 0.72 | 0.98 | -0.26 | -0.92 | -1.09 | -1.02 | -0.91 | -1.53 | -3.05 | -1.38 | -0.59 | -0.01 |
Nuclear Periphery | 6.49 | -5.32 | -0.71 | -9.13 | 5.18 | 0.91 | -5.65 | -8.92 | -11.17 | 4.83 | 2.32 | -8.64 | -10.23 | -14.18 | 7.17 |
Nuclear Periphery Foci | 3.90 | -6.19 | 1.30 | -7.56 | 7.17 | 0.63 | -3.69 | -6.77 | -6.83 | 3.38 | 1.86 | -8.03 | -8.74 | -10.57 | 7.25 |
Nucleolus | 0.61 | -3.12 | 1.01 | 1.20 | 6.61 | 1.03 | -4.08 | -0.31 | -1.21 | 3.78 | 0.87 | -7.03 | 0.43 | -0.42 | 7.42 |
Nucleus | 5.79 | -6.10 | -1.84 | -17.42 | 6.27 | 3.78 | -11.32 | -17.38 | -19.71 | 7.24 | 6.11 | -18.54 | -19.80 | -26.17 | 10.28 |
Peroxisomes | -4.22 | -1.02 | 0.57 | 8.26 | 2.89 | 3.37 | 5.98 | 9.57 | 6.56 | 0.33 | 1.48 | 6.49 | 10.80 | 10.53 | 2.22 |
Vacuole | 19.12 | 10.25 | 6.98 | -30.56 | -6.65 | -0.44 | -19.38 | -37.06 | -36.81 | 0.86 | 10.38 | -21.22 | -33.82 | -48.11 | -4.92 |
Vacuole Periphery | -5.90 | -1.12 | -1.19 | 5.32 | -0.20 | -1.05 | 14.27 | 3.22 | 3.72 | -3.04 | -2.49 | 11.04 | 5.08 | 6.49 | -2.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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