Standard name | |
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Human Ortholog | |
Description | Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 14 | 7 | 1 | 0 | 2 | 3 | – | 0 | 5 | 0 | 7 | 21 | 19 | 18 | 4 | 0 | 0 | 37 | 27 | 8 | 43 | 86 | 149 |
Bud | 7 | 5 | 5 | 5 | 9 | 20 | 26 | – | 19 | 29 | 23 | 29 | 16 | 21 | 32 | 7 | 7 | 7 | 3 | 3 | 0 | 3 | 5 | 8 |
Bud Neck | 3 | 0 | 11 | 5 | 4 | 4 | 8 | – | 3 | 2 | 3 | 5 | 8 | 11 | 5 | 2 | 6 | 1 | 4 | 8 | 0 | 14 | 14 | 21 |
Bud Site | 0 | 3 | 0 | 1 | 0 | 7 | 9 | – | 7 | 8 | 5 | 11 | 1 | 5 | 2 | 3 | 4 | 15 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 2 | – | 0 | 2 | 3 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 3 |
Cytoplasm | 155 | 80 | 35 | 102 | 104 | 62 | 108 | – | 31 | 37 | 32 | 32 | 156 | 284 | 204 | 6 | 2 | 8 | 54 | 35 | 11 | 50 | 58 | 53 |
Endoplasmic Reticulum | 2 | 8 | 1 | 0 | 0 | 4 | 0 | – | 1 | 1 | 1 | 4 | 2 | 4 | 8 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 2 |
Endosome | 28 | 4 | 5 | 6 | 18 | 2 | 49 | – | 1 | 15 | 11 | 2 | 16 | 51 | 90 | 15 | 27 | 17 | 64 | 30 | 23 | 58 | 63 | 69 |
Golgi | 3 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 2 | 5 | 2 | 1 | 1 | 4 | 5 |
Mitochondria | 29 | 29 | 0 | 12 | 3 | 79 | 42 | – | 94 | 93 | 109 | 115 | 8 | 6 | 5 | 10 | 11 | 9 | 82 | 43 | 40 | 72 | 127 | 89 |
Nucleus | 0 | 1 | 0 | 1 | 1 | 4 | 4 | – | 6 | 2 | 2 | 1 | 0 | 0 | 0 | 3 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 25 | 5 | 0 | 9 | 11 | 5 | 84 | – | 19 | 37 | 27 | 28 | 24 | 73 | 111 | 51 | 38 | 67 | 47 | 23 | 27 | 74 | 94 | 157 |
SpindlePole | 40 | 13 | 36 | 32 | 47 | 25 | 120 | – | 25 | 62 | 48 | 45 | 63 | 129 | 139 | 47 | 76 | 53 | 22 | 14 | 10 | 34 | 36 | 39 |
Vac/Vac Membrane | 5 | 1 | 0 | 0 | 2 | 2 | 6 | – | 1 | 1 | 1 | 1 | 0 | 2 | 3 | 1 | 2 | 0 | 4 | 7 | 0 | 2 | 4 | 3 |
Unique Cell Count | 257 | 147 | 82 | 157 | 176 | 157 | 336 | 159 | 196 | 190 | 193 | 264 | 518 | 488 | 116 | 125 | 122 | 332 | 203 | 132 | 364 | 511 | 613 | |
Labelled Cell Count | 298 | 165 | 100 | 175 | 199 | 218 | 462 | 210 | 295 | 266 | 282 | 315 | 607 | 620 | 160 | 186 | 186 | 332 | 203 | 132 | 364 | 511 | 613 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.9 | 4.9 | 4.8 | 5.3 | 3.8 | 4.7 | – | 4.1 | 4.3 | 4.1 | 4.2 | 4.5 | 4.4 | 4.9 | 6.4 | 6.3 | 6.5 | 3.9 | 4.2 | 4.3 |
Std Deviation (1e-4) | 0.8 | 0.6 | 1.5 | 2.2 | 1.8 | 1.7 | 1.8 | – | 1.6 | 1.5 | 1.4 | 1.2 | 1.1 | 0.9 | 1.1 | 1.4 | 1.2 | 1.5 | 1.2 | 1.6 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.12 | -0.36 | -0.05 | – | -0.26 | -0.19 | -0.24 | -0.23 | -0.11 | -0.16 | -0.01 | 0.38 | 0.36 | 0.4 | -0.33 | -0.21 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.5052 | -1.0525 | -1.4507 | -1.4538 | -1.4039 | -1.2502 | -0.0717 | 0.4504 | 0.354 | 0.5999 | 0.317 | 0.5823 | 2.7382 | 1.9341 | 2.0488 | 3.3364 | 2.2022 | 1.9146 |
Actin | 0.0198 | 0.0152 | 0.0219 | 0.0086 | 0.0105 | 0.0069 | 0.0078 | 0.025 | 0.0148 | 0.0192 | 0.0096 | 0.0167 | 0.0826 | 0.0463 | 0.0509 | 0.0072 | 0.0119 | 0.0054 |
Bud | 0.0003 | 0.002 | 0.0005 | 0.0003 | 0.0002 | 0.0005 | 0.0006 | 0.0034 | 0.0026 | 0.0012 | 0.0004 | 0.0003 | 0.0007 | 0.0019 | 0.001 | 0.0003 | 0.0037 | 0.0002 |
Bud Neck | 0.0023 | 0.0043 | 0.0324 | 0.0019 | 0.0128 | 0.0067 | 0.0039 | 0.0064 | 0.0083 | 0.0295 | 0.0206 | 0.0099 | 0.0043 | 0.0027 | 0.0288 | 0.0012 | 0.0012 | 0.0629 |
Bud Periphery | 0.0006 | 0.0027 | 0.001 | 0.0005 | 0.0005 | 0.0007 | 0.0015 | 0.0061 | 0.0049 | 0.0026 | 0.0009 | 0.0007 | 0.0011 | 0.002 | 0.0015 | 0.0011 | 0.0124 | 0.0004 |
Bud Site | 0.0023 | 0.0519 | 0.0105 | 0.001 | 0.0014 | 0.0022 | 0.0127 | 0.0421 | 0.0232 | 0.0106 | 0.0024 | 0.0003 | 0.0029 | 0.0708 | 0.0219 | 0.0004 | 0.0042 | 0.0006 |
Cell Periphery | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.001 | 0.0004 | 0.0002 | 0.0004 | 0.0003 |
Cytoplasm | 0.0294 | 0.0549 | 0.0254 | 0.0126 | 0.0081 | 0.0282 | 0.0296 | 0.0667 | 0.0435 | 0.0541 | 0.0285 | 0.0304 | 0.041 | 0.11 | 0.0436 | 0.0399 | 0.0457 | 0.0618 |
Cytoplasmic Foci | 0.3788 | 0.3348 | 0.3057 | 0.3051 | 0.3606 | 0.4075 | 0.3488 | 0.2975 | 0.2998 | 0.2914 | 0.3662 | 0.3576 | 0.2663 | 0.2103 | 0.263 | 0.2975 | 0.4061 | 0.422 |
Eisosomes | 0.0006 | 0.0008 | 0.0009 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0009 | 0.0008 | 0.0009 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0011 | 0.0016 | 0.0008 | 0.0002 | 0.0006 | 0.0004 | 0.001 | 0.0014 | 0.0011 | 0.0006 | 0.0009 | 0.0005 | 0.0056 | 0.0007 | 0.0009 | 0.0005 | 0.0035 | 0.0012 |
Endosome | 0.0603 | 0.0326 | 0.021 | 0.0253 | 0.0919 | 0.0242 | 0.0771 | 0.0555 | 0.0335 | 0.1126 | 0.0571 | 0.0359 | 0.0404 | 0.0305 | 0.0361 | 0.0168 | 0.057 | 0.0321 |
Golgi | 0.0141 | 0.0078 | 0.0071 | 0.0083 | 0.0143 | 0.0071 | 0.0156 | 0.0129 | 0.0095 | 0.0177 | 0.0118 | 0.0092 | 0.0226 | 0.0133 | 0.0226 | 0.0041 | 0.0103 | 0.0057 |
Lipid Particles | 0.0487 | 0.0479 | 0.0229 | 0.0246 | 0.1077 | 0.0426 | 0.0469 | 0.0177 | 0.015 | 0.0417 | 0.041 | 0.0172 | 0.0425 | 0.0264 | 0.0253 | 0.0347 | 0.0379 | 0.0262 |
Mitochondria | 0.0069 | 0.0049 | 0.0093 | 0.0033 | 0.0209 | 0.006 | 0.0106 | 0.0061 | 0.0089 | 0.007 | 0.0091 | 0.0081 | 0.0053 | 0.0048 | 0.0101 | 0.0046 | 0.029 | 0.0081 |
None | 0.1982 | 0.2863 | 0.2974 | 0.1843 | 0.0906 | 0.2461 | 0.1982 | 0.2198 | 0.2364 | 0.1551 | 0.1438 | 0.22 | 0.3235 | 0.3065 | 0.3568 | 0.4045 | 0.1997 | 0.2401 |
Nuclear Periphery | 0.0008 | 0.0011 | 0.0006 | 0.0004 | 0.0016 | 0.0004 | 0.0007 | 0.0005 | 0.0008 | 0.0007 | 0.0018 | 0.0003 | 0.0114 | 0.0002 | 0.0003 | 0.0002 | 0.0062 | 0.0008 |
Nucleolus | 0.0012 | 0.0026 | 0.0011 | 0.0142 | 0.0014 | 0.001 | 0.0017 | 0.0012 | 0.0007 | 0.004 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0005 | 0.0016 | 0.0007 |
Nucleus | 0.0022 | 0.003 | 0.0009 | 0.0164 | 0.0007 | 0.001 | 0.0011 | 0.0011 | 0.0009 | 0.0032 | 0.0007 | 0.0005 | 0.0022 | 0.0003 | 0.0004 | 0.0003 | 0.0048 | 0.0007 |
Peroxisomes | 0.2202 | 0.1195 | 0.2315 | 0.3836 | 0.257 | 0.2025 | 0.2282 | 0.2172 | 0.2839 | 0.2303 | 0.2914 | 0.2773 | 0.127 | 0.1661 | 0.1316 | 0.1809 | 0.1499 | 0.1166 |
Punctate Nuclear | 0.0094 | 0.0238 | 0.0071 | 0.0073 | 0.0161 | 0.0138 | 0.0104 | 0.0158 | 0.0097 | 0.0146 | 0.01 | 0.0133 | 0.0156 | 0.0038 | 0.0021 | 0.0028 | 0.0079 | 0.0112 |
Vacuole | 0.0021 | 0.0013 | 0.0013 | 0.0016 | 0.0017 | 0.0014 | 0.0025 | 0.0018 | 0.0011 | 0.0026 | 0.0022 | 0.0008 | 0.0017 | 0.001 | 0.001 | 0.0012 | 0.0038 | 0.0023 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0008 | 0.0009 | 0.0003 | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0025 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.0801 | 5.4496 | 2.6959 | 7.3938 | 5.2693 | 4.0619 | 3.6734 | 10.537 | 10.3533 | 6.5901 |
Translational Efficiency | 1.1208 | 1.3865 | 2.3504 | 1.4117 | 1.9255 | 1.8506 | 1.9139 | 0.9836 | 1.1293 | 1.9399 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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