Standard name | |
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Human Ortholog | |
Description | FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | 6 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 5 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 5 | 5 | 2 | 0 | 7 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 6 | 15 | 25 | 16 | 22 | 22 | 15 | 12 | 10 | 5 | 17 | 44 | 97 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 0 | 2 | 5 | 6 | 0 | 3 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 4 | 0 | 14 | 10 | 23 | 9 | 26 | 20 | 7 | 7 | 4 | 6 | 14 | 23 | 19 | 0 | 5 | 2 | 0 | 0 | 2 | 11 | 32 | 45 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 9 | 9 |
Mitochondria | 13 | 3 | 34 | 72 | 48 | 101 | 128 | 212 | 157 | 263 | 169 | 142 | 11 | 6 | 2 | 3 | 10 | 3 | 1 | 0 | 6 | 19 | 63 | 88 |
Nucleus | 5 | 0 | 7 | 19 | 18 | 14 | 24 | 50 | 16 | 27 | 24 | 21 | 55 | 54 | 76 | 1 | 10 | 1 | 2 | 1 | 0 | 2 | 6 | 5 |
Nuclear Periphery | 121 | 15 | 287 | 352 | 362 | 253 | 337 | 351 | 220 | 300 | 144 | 167 | 878 | 924 | 832 | 17 | 68 | 22 | 144 | 37 | 323 | 316 | 514 | 488 |
Nucleolus | 42 | 1 | 99 | 98 | 103 | 102 | 125 | 203 | 130 | 236 | 116 | 114 | 88 | 106 | 81 | 1 | 18 | 8 | 1 | 0 | 2 | 5 | 10 | 11 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 10 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | 4 | 4 | 2 | 2 | 15 | 40 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 5 |
Vac/Vac Membrane | 34 | 3 | 50 | 50 | 69 | 40 | 51 | 43 | 20 | 22 | 13 | 25 | 231 | 282 | 404 | 8 | 37 | 12 | 3 | 2 | 3 | 11 | 18 | 32 |
Unique Cell Count | 149 | 16 | 332 | 436 | 493 | 387 | 511 | 669 | 373 | 591 | 341 | 334 | 1048 | 1164 | 1229 | 21 | 105 | 34 | 159 | 42 | 349 | 376 | 682 | 719 |
Labelled Cell Count | 220 | 22 | 499 | 623 | 656 | 540 | 721 | 913 | 575 | 882 | 494 | 486 | 1304 | 1465 | 1570 | 30 | 154 | 50 | 159 | 42 | 349 | 376 | 682 | 719 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 7.9 | 9.6 | 9.3 | 9.1 | 7.4 | 7.3 | 7.1 | 7.0 | 6.4 | 6.4 | 6.7 | 10.1 | 10.0 | 10.2 | 7.6 | 8.0 | 10.5 | 11.2 | 11.8 | 12.4 |
Std Deviation (1e-4) | 1.9 | 1.7 | 2.5 | 2.7 | 2.9 | 2.3 | 2.0 | 2.4 | 2.0 | 1.8 | 1.9 | 1.8 | 1.8 | 2.0 | 2.4 | 1.4 | 2.0 | 2.0 | 2.1 | 2.3 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.08 | -0.38 | -0.4 | -0.44 | -0.46 | -0.57 | -0.58 | -0.51 | 0.07 | 0.06 | 0.09 | -0.33 | -0.27 | 0.13 | 0.23 | 0.3 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 2.4 | 1.8 | 2.0 | 1.3 | 1.7 | 0.2 | 1.0 | -0.3 | -0.2 | 1.8 | 4.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.5 | 0.3 | -1.4 | 0.6 | -1.0 | -1.8 | -3.0 | -2.4 | -1.8 | -3.2 | -2.3 | -3.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.5 | -0.2 | 5.4 | 5.3 | 7.4 | 9.5 | 10.7 | 11.1 | 9.4 | -8.2 | -9.7 | -10.9 | 0 | -0.2 | 0 |
Nucleus | 1.7 | 1.3 | 1.2 | 2.0 | 3.4 | 1.6 | 1.9 | 3.1 | 2.7 | 2.4 | 2.1 | 2.9 | 0 | 0 | 0 |
Nuclear Periphery | -2.1 | -4.5 | -6.5 | -6.6 | -10.5 | -8.1 | -10.8 | -12.0 | -10.1 | -1.2 | -2.9 | -6.7 | -0.7 | -5.0 | -3.3 |
Nucleolus | -2.3 | -2.9 | -1.0 | -1.7 | 0.2 | 1.4 | 3.1 | 1.2 | 1.2 | -9.9 | -9.7 | -11.8 | -2.5 | -2.6 | -0.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 |
Vacuole | -1.5 | -0.4 | -1.9 | -2.2 | -4.4 | -4.3 | -6.2 | -5.0 | -3.1 | 2.8 | 3.5 | 6.3 | 0 | 4.5 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.8784 | 7.5092 | 7.2485 | 7.431 | 6.4145 | 7.1294 | 6.8866 | 7.4808 | 6.7502 | 6.3083 | 6.4548 | 6.4033 | 5.9172 | 6.9973 | 6.4466 | 6.6676 | 5.6473 | 6.2754 |
Actin | 0.0093 | 0.0001 | 0.0111 | 0.0002 | 0.0003 | 0.0001 | 0.0197 | 0.0001 | 0.0011 | 0.0012 | 0.0022 | 0.0002 | 0.0045 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0062 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0053 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0014 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0065 | 0.0001 | 0.0017 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0004 | 0.0009 | 0.0001 | 0 | 0.0002 | 0 | 0.0031 | 0.0002 | 0.0041 | 0.003 | 0.0013 | 0.0048 | 0.0017 | 0 | 0.0004 | 0.0067 | 0.0125 | 0.0005 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0046 | 0.0008 | 0.0012 | 0.0015 | 0.0004 | 0.0001 | 0.0028 | 0.0006 | 0.0008 | 0.001 | 0.0007 | 0.0007 |
Endosome | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0071 | 0.0006 | 0.0026 | 0.0034 | 0.009 | 0.0015 | 0.0087 | 0.0002 | 0.0029 | 0.0044 | 0.002 | 0.0027 |
Golgi | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0021 | 0.0004 | 0.0016 | 0.0021 | 0.0183 | 0.0003 | 0.0014 | 0.0001 | 0.0003 | 0.0023 | 0.0015 | 0.0009 |
Lipid Particles | 0.0005 | 0.0031 | 0.0012 | 0.0005 | 0.0029 | 0.0002 | 0.003 | 0.0071 | 0.0119 | 0.014 | 0.0519 | 0.006 | 0.0164 | 0.0005 | 0.0014 | 0.0782 | 0.0432 | 0.0013 |
Mitochondria | 0.0008 | 0.0004 | 0.0004 | 0.0005 | 0.0014 | 0.0005 | 0.0051 | 0.0008 | 0.0018 | 0.0021 | 0.0433 | 0.0002 | 0.0032 | 0.0007 | 0.0016 | 0.0021 | 0.0024 | 0.0004 |
None | 0.0084 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0059 | 0.0001 | 0.0044 | 0.0005 | 0.0003 | 0.0003 | 0.0019 | 0.0003 | 0.001 | 0.0003 | 0.0004 | 0.0019 |
Nuclear Periphery | 0.9411 | 0.9106 | 0.9196 | 0.9121 | 0.9419 | 0.9715 | 0.8726 | 0.9175 | 0.8942 | 0.8966 | 0.7911 | 0.9672 | 0.904 | 0.9535 | 0.9356 | 0.8497 | 0.8987 | 0.9667 |
Nucleolus | 0.0003 | 0.0041 | 0.0003 | 0.0009 | 0.0117 | 0.0008 | 0.0011 | 0.0004 | 0.0016 | 0.001 | 0.0051 | 0.0002 | 0.002 | 0.0004 | 0.0007 | 0.0007 | 0.0012 | 0.0006 |
Nucleus | 0.0134 | 0.0558 | 0.0401 | 0.0616 | 0.0171 | 0.016 | 0.008 | 0.0228 | 0.0132 | 0.0053 | 0.0398 | 0.0027 | 0.0089 | 0.0026 | 0.0056 | 0.0024 | 0.0022 | 0.0041 |
Peroxisomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0007 | 0.0009 | 0.0011 | 0.001 | 0.0004 | 0 | 0.0001 | 0.0008 | 0.0012 | 0.0002 |
Punctate Nuclear | 0.0005 | 0.0031 | 0.0005 | 0.0004 | 0.0027 | 0.0005 | 0.0029 | 0.0006 | 0.0186 | 0.0213 | 0.0017 | 0.0019 | 0.0063 | 0.0004 | 0.0012 | 0.0037 | 0.0066 | 0.0017 |
Vacuole | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0044 | 0.0006 | 0.0008 | 0.0006 | 0.0012 | 0.0001 | 0.0016 | 0.0004 | 0.0011 | 0.0005 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0195 | 0.0203 | 0.0249 | 0.0228 | 0.0185 | 0.0098 | 0.0443 | 0.0478 | 0.0398 | 0.0458 | 0.0325 | 0.0134 | 0.034 | 0.0402 | 0.0455 | 0.0465 | 0.0263 | 0.0112 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.6285 | 44.4478 | 25.7532 | 41.2129 | 33.5576 | 23.1451 | 29.8582 | 34.2796 | 30.7988 | 36.5373 |
Translational Efficiency | 1.6764 | 1.2761 | 2.168 | 1.2251 | 1.5952 | 2.014 | 1.4814 | 1.4656 | 1.6473 | 1.5745 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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