Standard name
Human Ortholog
Description Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.17 0.17 0.3 0.15 0.13 0.13 0.08 0.11 0.08 0.08 0.27 0.34 0.42 0.1 0.08 0.08 0 0 0.05 0.13 0.15 0.18
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.3 0.08 0.23 0.14 0.52 0.63 0.59 0.67 0.74 0.81 0.8 0 0 0 0 0.06 0.07 0 0 0.05 0 0 0
Nucleus 0.66 0.66 0.65 0.59 0.48 0.48 0.47 0.51 0.48 0.43 0.42 0.44 0.62 0.52 0.48 0.81 0.83 0.83 0.52 0.67 0.45 0.49 0.39 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.23 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.11 0.07 0.07 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.08 0.09 0.1 0.13
Vac/Vac Membrane 0 0.09 0.07 0 0.07 0.06 0.06 0.05 0 0.07 0.09 0 0.05 0.1 0.09 0.07 0 0.05 0.14 0.11 0.14 0.11 0.14 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud 1 0 0 0 1 1 2 1 3 10 2 4 0 0 0 0 0 0 4 0 2 2 6 13
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 1 4 0 1 0 0 0 0 0 2 5 8
Bud Site 0 0 0 0 0 3 9 7 4 6 6 6 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 0 18 19 31 25 30 34 19 36 16 18 59 138 176 18 16 9 6 0 7 28 55 66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 3 3 2 1 0 1 0 2 3
Endosome 15 1 5 5 8 2 0 3 0 0 0 0 11 21 10 1 2 1 6 1 5 3 7 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 1 1 1 0 1 0
Mitochondria 35 13 8 25 15 86 146 159 155 240 161 180 0 2 2 7 11 8 6 2 6 5 15 16
Nucleus 108 29 68 65 50 80 108 138 110 139 83 99 134 210 200 145 156 98 113 55 61 111 145 118
Nuclear Periphery 3 0 0 0 0 0 1 1 2 0 0 4 0 0 0 0 0 0 0 0 0 2 1 2
Nucleolus 33 10 2 0 1 5 9 5 8 7 5 9 1 0 1 4 4 4 23 7 14 15 27 25
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 10 0 5 3 0 3 2 2 2 2 2 0 9 12 9 1 3 0 16 2 11 20 37 49
Vac/Vac Membrane 4 4 7 3 7 10 15 13 10 24 17 8 11 40 37 13 8 6 31 9 19 23 53 51
Unique Cell Count 163 44 104 110 104 166 231 271 231 326 198 225 216 404 415 179 189 118 220 83 135 225 372 376
Labelled Cell Count 219 57 113 120 114 215 322 363 313 464 292 329 227 429 439 194 205 128 220 83 135 225 372 376


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.3 4.8 4.4 4.8 3.9 3.7 3.6 3.5 3.4 3.2 3.2 6.1 6.1 5.8 8.8 8.4 8.8 6.1 6.5 6.4
Std Deviation (1e-4) 0.8 1.2 1.4 1.8 2.0 1.7 1.2 1.2 1.0 0.9 1.0 1.0 1.1 1.2 1.3 1.6 1.7 1.6 1.4 1.6 1.5
Intensity Change (Log2) – – – -0.12 -0.03 -0.33 -0.38 -0.41 -0.48 -0.52 -0.61 -0.58 0.32 0.32 0.26 0.85 0.79 0.86 0.32 0.41 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.1 -0.5 -1.0 -1.2 -2.5 -1.7 -2.4 -2.5 2.0 3.3 4.7 -1.8 -2.3 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.1 0.2 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -1.4 -0.6 -0.3
Nucleus -0.9 -2.5 -2.8 -3.2 -2.5 -3.0 -4.0 -3.9 -3.6 -0.6 -2.4 -3.1 2.9 3.3 3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 -0.2 -0.1 -0.7 -0.9 0.2 0.6 0 -0.6 1.0 0.7 0.2 -0.9 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5427 0.9029 1.1816 0.5768 0.8882 0.9922 1.1727 0.7486 0.6966 1.4467 0.7671 0.918 0.1604 0.3707 0.4241 0.392 0.0828 0.1006
Actin 0.0499 0.0002 0.0039 0.0002 0.0927 0.0002 0.0698 0.002 0.0023 0.0003 0.0041 0.0236 0.0131 0.0002 0.0066 0.0262 0.0052 0.0035
Bud 0.0002 0.0001 0.0009 0.0001 0.0003 0.0003 0.0011 0.0006 0.0006 0.0002 0.0003 0.001 0.0004 0.0002 0.0002 0.0003 0.0002 0.0002
Bud Neck 0.0031 0.0005 0.0045 0.0006 0.0043 0.0042 0.0061 0.0046 0.0008 0.0006 0.0016 0.0032 0.0021 0.0007 0.0006 0.0007 0.0032 0.003
Bud Periphery 0.0003 0.0001 0.0009 0.0001 0.0005 0.0003 0.0014 0.0005 0.0007 0.0001 0.0005 0.0021 0.0006 0.0001 0.0003 0.0008 0.0004 0.0002
Bud Site 0.0022 0.0014 0.0205 0.0002 0.0013 0.0003 0.0054 0.0061 0.0022 0.0011 0.0026 0.0012 0.0012 0.0007 0.0007 0.0004 0.0006 0.0002
Cell Periphery 0.0002 0.0001 0.0004 0 0.0001 0 0.0005 0.0001 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.0249 0.021 0.035 0.0265 0.0013 0.0382 0.0876 0.0672 0.0363 0.07 0.1334 0.1107 0.0134 0.0144 0.0152 0.0014 0.0332 0.0087
Cytoplasmic Foci 0.0109 0.0008 0.0081 0.0048 0.0017 0.0037 0.0439 0.0022 0.01 0.0288 0.0205 0.0196 0.0056 0.0006 0.0034 0.0004 0.0018 0.0022
Eisosomes 0.0008 0 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0.0003 0 0
Endoplasmic Reticulum 0.0018 0.0007 0.0021 0.0005 0.0002 0.0007 0.0092 0.0014 0.001 0.0014 0.0027 0.0022 0.0024 0.0003 0.0013 0.0011 0.0053 0.0005
Endosome 0.0061 0.0008 0.011 0.0004 0.0009 0.0029 0.0603 0.002 0.0108 0.0066 0.0167 0.0164 0.0142 0.0004 0.0064 0.0033 0.0104 0.0018
Golgi 0.0048 0 0.0067 0 0.0019 0.0001 0.0272 0.0005 0.0062 0.0014 0.0103 0.0144 0.0035 0 0.0022 0.003 0.001 0.0006
Lipid Particles 0.0112 0.0002 0.0039 0.0002 0.0007 0.0002 0.0306 0.0003 0.0023 0.0085 0.1057 0.0313 0.0092 0 0.0036 0.0021 0.0006 0.0024
Mitochondria 0.0028 0.0002 0.0032 0.0003 0.0012 0.0003 0.03 0.0008 0.0039 0.0445 0.0154 0.0137 0.003 0.0002 0.008 0.0026 0.0008 0.0008
None 0.097 0.0362 0.0621 0.0796 0.0007 0.0534 0.0301 0.0305 0.0527 0.026 0.1473 0.0216 0.0227 0.0153 0.0515 0.0012 0.0514 0.0132
Nuclear Periphery 0.0124 0.0061 0.0115 0.0058 0.0069 0.0028 0.0441 0.0146 0.0077 0.0074 0.0112 0.0149 0.0235 0.0041 0.0167 0.0076 0.0285 0.0165
Nucleolus 0.0167 0.0179 0.0168 0.009 0.0366 0.0481 0.0109 0.0169 0.0155 0.0058 0.0089 0.0219 0.0213 0.0171 0.0181 0.0089 0.0616 0.0364
Nucleus 0.6762 0.896 0.7152 0.8342 0.7278 0.8046 0.4729 0.8084 0.7852 0.7042 0.4538 0.6557 0.8224 0.916 0.8205 0.9252 0.6857 0.8501
Peroxisomes 0.0103 0 0.0012 0 0.0016 0.0001 0.0232 0.0002 0.0089 0.0047 0.0267 0.0107 0.0036 0 0.0036 0.0003 0.0002 0.005
Punctate Nuclear 0.0669 0.0167 0.0877 0.0374 0.1177 0.0382 0.0344 0.039 0.0503 0.0873 0.0282 0.0325 0.0337 0.0291 0.0392 0.013 0.108 0.0533
Vacuole 0.001 0.001 0.0036 0.0002 0.0012 0.0013 0.0075 0.0016 0.0015 0.0006 0.0077 0.0018 0.0027 0.0004 0.0011 0.0003 0.0013 0.0006
Vacuole Periphery 0.0004 0.0001 0.0005 0.0001 0.0003 0.0001 0.0034 0.0004 0.0007 0.0003 0.0017 0.0012 0.0014 0.0001 0.0006 0.0008 0.0006 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8113 9.2401 13.1417 15.4772 9.4927 26.6874 13.0407 12.3485 17.1432 20.6074
Translational Efficiency 0.8023 1.0656 0.5712 0.8538 1.2942 0.724 0.8198 0.6109 0.605 0.6561

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Swd1

Swd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available