Standard name
Human Ortholog
Description Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0.05 0 0 – 0 0 0 0 0.06 0 0.08 0.07 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0.05 0 0 0 0 0.12 0 0 0 0 0 0 0.1 0.11 0.1
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0.06
Mitochondria 0.07 0 – 0 0 0 0.05 – 0.22 0.15 0.22 0.18 0 0 0 0.06 0 0 0 0 0 0 0.08 0.09
Nucleus 0.7 0.72 – 0.8 0.73 0.71 0.62 – 0.41 0.34 0.42 0.34 0.74 0.73 0.54 0.68 0.74 0.65 0.56 0.58 0.7 0.37 0.34 0.27
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.28 – 0.17 0.2 0.19 0.28 – 0.4 0.44 0.44 0.41 0.15 0.13 0.19 0.13 0.11 0.11 0.09 0.14 0.13 0 0 0.05
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0.07 0 0.05 0.07 0 0 0 0.05 0 0 0 0.05 0.12
SpindlePole 0 0 – 0 0 0 0 – 0 0.07 0 0.08 0.05 0.07 0.11 0 0 0 0 0 0 0.22 0.06 0.05
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0.05 0 0 0 0 0 0.05 0.1 0.07 0.1 0.11 0.13 0.07 0.15 0.23 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 0 2 0 1 0 1 1 1 0 0 1 6
Bud 1 0 – 0 0 0 0 – 0 6 2 3 0 1 0 0 0 0 3 0 1 0 5 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 – 0 0 0 0 – 1 6 8 8 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 – 4 13 7 5 – 2 3 2 5 5 6 13 11 18 33 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 4 4 7 0 0 0 0 0 0
Endosome 5 0 – 1 2 4 15 – 3 15 1 5 1 5 20 0 2 1 9 1 1 2 23 28
Golgi 0 0 – 0 1 0 1 – 0 0 0 0 0 1 1 11 2 8 2 0 0 1 4 16
Mitochondria 14 0 – 2 4 9 18 – 42 50 48 45 1 1 2 9 6 5 8 1 0 0 18 24
Nucleus 133 34 – 121 185 173 206 – 79 112 91 84 64 98 90 106 165 179 120 48 36 10 75 73
Nuclear Periphery 5 1 – 2 1 3 5 – 1 10 2 8 0 1 3 0 0 0 1 1 0 0 3 3
Nucleolus 54 13 – 26 51 46 92 – 78 146 95 100 13 18 31 20 24 31 20 11 6 0 9 13
Peroxisomes 1 0 – 0 4 1 5 – 8 14 6 18 3 7 12 1 1 6 11 1 0 0 11 33
SpindlePole 4 0 – 4 4 3 3 – 7 24 8 20 4 9 18 1 0 4 9 3 0 6 12 13
Vac/Vac Membrane 3 2 – 4 7 8 14 – 10 8 6 7 3 3 8 15 15 27 24 10 3 4 49 51
Unique Cell Count 190 47 152 254 242 334 195 332 218 246 87 135 167 156 223 277 215 83 52 29 219 272
Labelled Cell Count 220 52 164 272 254 365 231 394 269 303 95 152 199 179 237 302 215 83 52 29 219 272


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 16.9 – 15.1 18.5 16.9 17.1 – 15.4 15.8 14.0 15.1 16.9 19.4 18.8 34.7 33.9 31.7 – 12.3 12.9
Std Deviation (1e-4) 3.9 5.6 – 5.3 5.2 4.8 6.2 – 4.5 5.6 4.5 6.0 8.1 7.5 9.0 14.6 12.1 14.3 – 2.6 3.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3646 11.9249 9.9813 7.8792 8.0751 11.9546 9.9573 14.8758 13.4959 9.8477 12.3295 12.4138 7.1601 12.029 11.2905 10.5938 6.3462 9.0142
Actin 0.0496 0.0088 0.0253 0.0043 0.0045 0.002 0.0053 0.0008 0.0017 0.0208 0.0056 0.003 0.0617 0.0045 0.0301 0.0147 0.0459 0.0421
Bud 0.0011 0.0008 0.0005 0.0013 0.0002 0.0018 0.0007 0.0002 0.0004 0.0004 0.0005 0.0005 0.0006 0.0015 0.0012 0.0004 0.0014 0.0025
Bud Neck 0.0008 0.0009 0.0007 0.0015 0.0009 0.0037 0.0004 0.0003 0.0005 0.0004 0.0008 0.001 0.0011 0.0007 0.0007 0.0024 0.0024 0.0181
Bud Periphery 0.0024 0.0024 0.0013 0.0039 0.001 0.0058 0.0014 0.0009 0.001 0.0006 0.0007 0.0021 0.0009 0.0035 0.0016 0.0005 0.0031 0.0059
Bud Site 0.0025 0.002 0.0009 0.0015 0.0004 0.0009 0.002 0.0004 0.0006 0.001 0.0013 0.0003 0.0066 0.0035 0.009 0.0011 0.0037 0.0022
Cell Periphery 0.0006 0.0009 0.0004 0.0015 0.0006 0.001 0.0001 0.0004 0.0003 0.0001 0.0001 0.0006 0.0005 0.0005 0.0006 0.0001 0.0004 0.001
Cytoplasm 0.0051 0.0005 0.0008 0.0034 0.0003 0.0006 0.0045 0.0001 0.0005 0.0007 0.001 0.0002 0.0052 0.0003 0.0008 0.0003 0.0007 0.0009
Cytoplasmic Foci 0.0344 0.0061 0.0039 0.0278 0.0272 0.0013 0.0112 0.0004 0.0034 0.0738 0.004 0.0017 0.0415 0.0346 0.0165 0.0359 0.0531 0.0096
Eisosomes 0.0005 0.0003 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0005 0.0001 0.0005 0.0004
Endoplasmic Reticulum 0.0034 0.0003 0.0006 0.001 0.0002 0.0002 0.0025 0.0001 0.0001 0.0004 0.0003 0.0001 0.0034 0.0002 0.0004 0.0001 0.0007 0.0011
Endosome 0.0162 0.003 0.0057 0.012 0.0027 0.0021 0.0095 0.0009 0.0009 0.0097 0.0018 0.0006 0.0118 0.0014 0.0111 0.0022 0.0322 0.0159
Golgi 0.0095 0.0099 0.0059 0.0036 0.0082 0.0011 0.0019 0.0005 0.0007 0.0121 0.0016 0.0018 0.0112 0.0032 0.0228 0.0093 0.0556 0.0176
Lipid Particles 0.0556 0.0219 0.026 0.0349 0.2876 0.0149 0.0065 0.0038 0.0098 0.0126 0.0049 0.0516 0.0802 0.0807 0.0374 0.0104 0.1164 0.1049
Mitochondria 0.0398 0.0252 0.0275 0.1159 0.0795 0.01 0.008 0.0083 0.0251 0.0049 0.0051 0.031 0.0284 0.0303 0.0412 0.0067 0.0561 0.04
None 0.0194 0.0004 0.0007 0.0026 0.0004 0.0005 0.0031 0.0002 0.0005 0.0005 0.0008 0.0004 0.0025 0.0005 0.0008 0.0003 0.001 0.0008
Nuclear Periphery 0.0553 0.0609 0.0754 0.0561 0.0197 0.0355 0.0237 0.0182 0.0204 0.0767 0.0704 0.0293 0.0407 0.0184 0.0787 0.0572 0.0225 0.0656
Nucleolus 0.1778 0.2622 0.2107 0.0987 0.0682 0.2495 0.1453 0.1749 0.1884 0.0671 0.095 0.2659 0.1587 0.2095 0.1783 0.1846 0.2587 0.1979
Nucleus 0.3913 0.5299 0.5484 0.3807 0.4073 0.6445 0.7123 0.7497 0.6681 0.5908 0.5818 0.5708 0.4531 0.5503 0.4904 0.3844 0.1774 0.4167
Peroxisomes 0.0379 0.0047 0.0035 0.006 0.0387 0.001 0.0019 0.0004 0.0008 0.007 0.0028 0.0017 0.0191 0.0062 0.006 0.0211 0.1146 0.0129
Punctate Nuclear 0.079 0.0495 0.0554 0.2175 0.0369 0.0089 0.0562 0.0312 0.0687 0.1174 0.2193 0.019 0.0582 0.0426 0.06 0.2649 0.0484 0.0214
Vacuole 0.0048 0.0027 0.0013 0.0107 0.003 0.0082 0.0015 0.003 0.0015 0.0006 0.0006 0.0026 0.0056 0.0018 0.002 0.0004 0.0024 0.0076
Vacuole Periphery 0.0128 0.0068 0.0048 0.015 0.0121 0.0064 0.0019 0.005 0.0063 0.0021 0.0015 0.0155 0.0087 0.0055 0.0098 0.003 0.0028 0.015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 111.7596 24.3158 24.7116 52.0624 67.4672 80.9661 28.9951 20.3814 39.3546 77.5565
Translational Efficiency 1.8927 2.1745 1.3867 1.5583 2.1469 2.8681 1.5893 1.9465 1.7331 1.671

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 985 209 566 2272 1069 1018 107 2394 2054 1227 673

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1199.00 1339.40 1909.66 2162.48 1283.76 1260.32 1767.98 2232.48 1279.44 1298.24 1792.11 2173.61
Standard Deviation 432.40 298.76 449.02 697.37 246.11 262.76 466.97 693.06 259.54 283.37 467.01 697.16
Intensity Change Log 2 — 0.159755 0.671484 0.850855 — -0.026585 0.461726 0.798272 — 0.066411 0.566835 0.823905

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001143 0.005490 0.005499 0.007432 0.006385 0.002937 0.003277 0.010330 0.006118 0.004162 0.003655 0.007893
Bud Neck 0.008613 0.021490 0.033047 0.042954 0.022943 0.022161 0.029723 0.042169 0.022212 0.021840 0.030289 0.042829
Bud Site 0.003273 0.007709 0.012202 0.024244 0.007282 0.006328 0.009032 0.038581 0.007078 0.006990 0.009572 0.026523
Cell Periphery 0.000088 0.000143 0.000158 0.000384 0.000193 0.000209 0.000160 0.000192 0.000187 0.000177 0.000160 0.000354
Cytoplasm 0.000476 0.000410 0.000349 0.001927 0.000421 0.000364 0.000557 0.000972 0.000424 0.000386 0.000521 0.001775
Cytoplasmic Foci 0.128115 0.017446 0.016040 0.071292 0.012572 0.007136 0.011040 0.080025 0.018460 0.012081 0.011892 0.072681
Eisosomes 0.000175 0.000406 0.000375 0.000211 0.000443 0.000244 0.000200 0.000304 0.000429 0.000322 0.000230 0.000225
Endoplasmic Reticulum 0.002441 0.005426 0.002070 0.001638 0.006857 0.004316 0.002014 0.001604 0.006632 0.004848 0.002024 0.001633
Endosome 0.000884 0.000491 0.000500 0.006985 0.000657 0.000663 0.000515 0.012159 0.000668 0.000581 0.000512 0.007808
Golgi 0.000408 0.000775 0.000603 0.007542 0.000662 0.000859 0.000345 0.011865 0.000649 0.000818 0.000389 0.008229
Lipid Particles 0.000691 0.008192 0.010362 0.021447 0.012904 0.002695 0.000418 0.032853 0.012281 0.005331 0.002112 0.023260
Mitochondria 0.001986 0.009554 0.011533 0.013436 0.013122 0.005655 0.007901 0.007706 0.012555 0.007524 0.008520 0.012525
Mitotic Spindle 0.052951 0.123878* 0.220969* 0.308088* 0.111667* 0.035005 0.093868 0.313617* 0.108674* 0.077624 0.115518* 0.308967*
None 0.002259 0.001377 0.001221 0.004430 0.001423 0.001640 0.001426 0.001386 0.001465 0.001514 0.001391 0.003946
Nuclear Periphery 0.000970 0.002084 0.002192 0.002775 0.002697 0.000537 0.001089 0.012646 0.002609 0.001279 0.001277 0.004344
Nuclear Periphery Foci 0.006778 0.009039 0.003735 0.005055 0.009046 0.002493 0.001435 0.012555 0.008930 0.005632 0.001827 0.006248
Nucleolus 0.151723 0.280244* 0.153278 0.112814 0.285198* 0.190863 0.142397 0.114967 0.278396* 0.233726* 0.144250 0.113156
Nucleus 0.588074* 0.405712* 0.458394* 0.297461* 0.413525* 0.678322* 0.660085* 0.210447* 0.422420* 0.547591* 0.625730* 0.283627*
Peroxisomes 0.047596 0.097687 0.065334 0.060887 0.089635 0.035509 0.032599 0.066757 0.087493 0.065326 0.038175 0.061820
Vacuole 0.001082 0.001843 0.001700 0.006604 0.001762 0.001473 0.001434 0.014168 0.001728 0.001650 0.001479 0.007806
Vacuole Periphery 0.000271 0.000603 0.000438 0.002394 0.000608 0.000591 0.000485 0.014696 0.000591 0.000597 0.000477 0.004350

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.33 -6.73 -5.69 -1.11 -0.38 6.12 5.82 -1.22 -1.96 -1.84 4.03 4.51 -1.16 -2.79 -2.91
Bud Neck -5.75 -6.40 -10.54 -7.64 -2.41 -0.29 -5.69 -3.59 -3.50 -2.02 -0.24 -6.79 -8.53 -8.36 -4.09
Bud Site -4.08 -4.25 -9.90 -8.46 -2.85 -0.33 -4.19 -3.77 -3.73 -3.22 -0.73 -5.34 -9.36 -9.07 -6.50
Cell Periphery -2.83 -3.49 -5.02 -4.16 -2.83 -0.34 -0.12 -2.39 -1.29 -2.21 0.24 -0.48 -3.72 -3.46 -3.43
Cytoplasm 0.93 1.15 -6.11 -7.41 -7.37 -0.40 -2.82 -3.13 -2.91 -1.88 -0.11 -2.51 -7.74 -7.70 -6.42
Cytoplasmic Foci 5.19 5.18 3.31 -7.00 -5.84 1.95 -1.13 -3.73 -4.01 -3.48 2.79 1.33 -7.49 -8.82 -8.02
Eisosomes -4.70 -3.30 -0.69 6.69 3.35 4.66 9.99 1.98 -0.95 -1.36 3.88 7.40 9.36 3.34 1.68
Endoplasmic Reticulum -4.46 -0.86 0.32 5.41 1.33 4.51 11.51 7.15 3.27 -0.15 3.57 11.34 11.82 7.11 1.17
Endosome 0.54 0.56 -2.71 -3.28 -3.27 -0.16 0.67 -1.32 -1.31 -1.34 0.45 0.86 -3.22 -3.27 -3.33
Golgi -2.86 -1.95 -2.74 -2.52 -2.56 -0.67 3.06 -1.52 -1.46 -1.56 -1.11 2.28 -3.04 -2.88 -3.14
Lipid Particles -4.45 -1.52 -3.61 -1.36 -1.21 5.15 7.94 -0.70 -1.72 -1.98 3.86 6.52 -0.58 -2.59 -3.76
Mitochondria -7.80 -5.60 -7.86 -2.16 -0.53 7.16 5.30 1.82 -1.66 -0.32 6.00 4.16 0.26 -3.64 -2.39
Mitotic Spindle -3.78 -4.38 -9.46 -7.97 -2.93 10.52* 1.49 -4.35 -6.42 -4.68 4.14 -0.34 -10.54 -12.59 -9.66
None 0.90 0.88 -1.00 -1.60 -1.59 -0.71 -1.12 -0.35 0.14 0.03 -0.33 -0.69 -1.56 -1.50 -1.48
Nuclear Periphery -1.93 -1.27 -2.35 -0.97 -0.93 8.55 4.52 -1.36 -1.87 -1.69 5.18 4.08 -1.07 -2.85 -2.64
Nuclear Periphery Foci -0.93 0.65 0.66 3.07 -0.12 4.82 6.68 0.08 -1.36 -1.68 2.49 6.32 2.85 1.01 -1.75
Nucleolus -6.11 -1.11 0.74 11.93 2.40 8.95 13.73 7.34 3.19 1.36 4.90 13.48 14.54 10.38 2.57
Nucleus 3.62 1.96 5.52 4.24 4.36 -19.09 -14.35 4.76 11.75 10.30 -10.51 -12.67 7.14 13.95 15.62
Peroxisomes -3.91 -1.12 -0.05 6.08 1.48 11.39 12.26 1.93 -2.04 -2.07 4.84 10.94 5.77 1.64 -3.14
Vacuole -1.91 -2.13 -3.82 -3.48 -2.75 0.56 -0.53 -2.33 -2.35 -2.30 -0.03 -1.21 -4.23 -4.23 -4.01
Vacuole Periphery -4.47 -4.02 -2.36 -1.75 -1.94 0.20 1.22 -1.11 -1.11 -1.13 -0.16 1.20 -1.73 -1.72 -1.80
Standard name
Human Ortholog
Description Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (51%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rfa1

Rfa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available